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Application of synergistic β-lactamase inhibitors and antibiotics in the treatment of wounds infected by superbugs

Authors :
Xiaoyuan Ding
Jing Su
Xiao Chen
Shizhong Zhang
Mengkao Li
Jie yang
Zekun Wang
Jingyuan Wang
Weiyun Wang
Dongdong Sun
Guojun Wang
Source :
Arabian Journal of Chemistry, Vol 15, Iss 7, Pp 103936- (2022)
Publication Year :
2022
Publisher :
Elsevier, 2022.

Abstract

Amoxicillin appears to be clinically drug-resistant due to the presence of β-lactamase in bacteria. Here, we designed and prepared a hollow Prussian Blue (HPB)-based therapeutic nanoplatform that was constructed by encapsulating amoxicillin into polyethyleneimine with β-lactamase inhibitor 4-carboxyphenylboronic acid (4-Cpba) decorated HPB nanoparticles (CPA NPs). The antibacterial effect of the CPA NPs on drug-resistant bacteria was observed by in vitro colony-forming unit, minimum inhibitory concentration, scanning electron microscopy, and fluorescence tests. The results show that amoxicillin effectively inhibited Escherichia coli and Staphylococcus aureus-resistant bacteria in the presence of 4-Cpba. The in vivo experimental results show that the CPA NPs exhibited a synergistic anti-infective effect in vivo, which inhibited the inflammatory response and apoptosis induced by the drug-resistant bacterial infection, and promoted wound healing in mice. The hematoxylin and eosin staining and blood biochemical experiments revealed that the acute toxicity of the material was negligible and it had good biocompatibility. Our results verify our design that CPA NPs can restore the antibacterial activity of amoxicillin.

Details

Language :
English
ISSN :
18785352
Volume :
15
Issue :
7
Database :
Directory of Open Access Journals
Journal :
Arabian Journal of Chemistry
Publication Type :
Academic Journal
Accession number :
edsdoj.80651aa15bf4196b03127d68d6e1ba4
Document Type :
article
Full Text :
https://doi.org/10.1016/j.arabjc.2022.103936