1. Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn
- Author
-
Jianzhi Sun, Yuqiang Liu, Shi-Zhao Xu, Qian Cai, and Xin Lv
- Subjects
iridoids ,Gentiana radix ,Pharmaceutical Science ,wine-processing ,Mass spectrometry ,Orbitrap ,01 natural sciences ,Analytical Chemistry ,law.invention ,lcsh:QD241-441 ,Triterpenoid ,lcsh:Organic chemistry ,law ,Drug Discovery ,Physical and Theoretical Chemistry ,UHPLC-LTQ-Orbitrap ,xanthones ,Wine ,Chromatography ,biology ,010405 organic chemistry ,Chemistry ,010401 analytical chemistry ,Organic Chemistry ,biology.organism_classification ,Mass measurement ,0104 chemical sciences ,Highly sensitive ,Chemistry (miscellaneous) ,Chemical constituents ,flavonoids ,characteristic fragmentation pathways ,Molecular Medicine ,Gentiana - Abstract
Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed . Based on the accurate mass measurement (<, 5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of . By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different.
- Published
- 2018
- Full Text
- View/download PDF