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MEK1-independent activation of MAPK and MEK1-dependent activation of p70 S6 kinase by stem cell factor (SCF) in ovarian cancer cells
- Source :
- Biochemical and Biophysical Research Communications. 382:385-389
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- We discovered a stem cell factor (SCF)-triggered, MEK1-independent, and PI3K-dependent MAPK activation pathway in the Kit-expressing ovarian cancer cell line HEY. When we knocked down MEK1 with RNA interference (RNAi) to study the function of MEK1 on the proliferation and survival of ovarian cancer cells, we found that impaired cell growth still occurred after MEK1 expression had been suppressed, although MAPK activation remained intact. This suggests that there is MEK1-independent activation of MAPK in the SCF-induced ovarian cancer cell growth process, and that MEK1 still plays a crucial role in maintaining the malignant properties of ovarian cancer cells even when it fails to activate MAPK as expected.
- Subjects :
- MAPK/ERK pathway
Rex1
MAP Kinase Kinase 1
Biophysics
Cell Growth Process
Stem cell factor
Biology
environment and public health
Biochemistry
Cancer stem cell
Cell Line, Tumor
medicine
Humans
Molecular Biology
Cell Proliferation
Mitogen-Activated Protein Kinase Kinases
Ovarian Neoplasms
Stem Cell Factor
Cell growth
Ribosomal Protein S6 Kinases, 70-kDa
Cell Biology
medicine.disease
Cell biology
Enzyme Activation
Gene Knockdown Techniques
embryonic structures
Cancer research
Female
RNA Interference
biological phenomena, cell phenomena, and immunity
Stem cell
Ovarian cancer
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 382
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....573228bcfe2f4d6c51138e8f57101d1b