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UPLC-MS/MS of Atractylenolide I, Atractylenolide II, Atractylenolide III, and Atractyloside A in Rat Plasma after Oral Administration of Raw and Wheat Bran-Processed Atractylodis Rhizoma
- Source :
- Molecules, Volume 23, Issue 12, Molecules, Vol 23, Iss 12, p 3234 (2018)
- Publication Year :
- 2018
-
Abstract
- Atractylodis Rhizoma is the dried rhizome of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz and is often processed by stir-frying with wheat bran to reduce its dryness and increase its spleen tonifying activity. However, the mechanism by which the processing has this effect remains unknown. To explain the mechanism based on the pharmacokinetics of the active compounds, a rapid, sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed to analyze atractylenolides I, II, and III, and atractyloside A simultaneously in rat plasma after oral administration of raw and processed Atractylodis Rhizoma. Acetaminophen was used as the internal standard and the plasma samples were pretreated with methanol. Positive ionization mode coupled with multiple reaction monitoring mode was used to analyze the four compounds. The method validation revealed that all the calibration curves displayed good linear regression over the concentration ranges of 3.2&ndash<br />350, 4&ndash<br />500, 4&ndash<br />500, and 3.44&ndash<br />430 ng/mL for atractylenolides I, II, and III, and atractyloside A, respectively. The relative standard deviations of the intra- and inter-day precisions of the four compounds were less than 6% with accuracies (relative error) below 2.38%, and the extraction recoveries were more than 71.90 &plusmn<br />4.97%. The main pharmacokinetic parameters of the four compounds were estimated with Drug and Statistics 3.0 and the integral pharmacokinetics were determined based on an area under the curve weighting method. The results showed that the integral maximum plasma concentration and area under the curve increased after oral administration of processed Atractylodis Rhizoma.
- Subjects :
- Dietary Fiber
Calibration curve
wheat bran processing
Pharmaceutical Science
Administration, Oral
Atractylodis Rhizoma
integral pharmacokinetics
Atractyloside
01 natural sciences
Article
Analytical Chemistry
lcsh:QD241-441
Rats, Sprague-Dawley
03 medical and health sciences
Lactones
0302 clinical medicine
lcsh:Organic chemistry
Pharmacokinetics
Oral administration
Limit of Detection
Tandem Mass Spectrometry
Drug Discovery
Animals
Physical and Theoretical Chemistry
Chromatography, High Pressure Liquid
Chromatography
biology
Bran
Chemistry
010401 analytical chemistry
Organic Chemistry
Selected reaction monitoring
Area under the curve
Reproducibility of Results
Atractylodes
biology.organism_classification
0104 chemical sciences
Chemistry (miscellaneous)
UPLC-MS/MS
030220 oncology & carcinogenesis
Area Under Curve
Linear Models
Molecular Medicine
Atractylodes lancea
Sesquiterpenes
Rhizome
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 23
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....f5de06444de56e1b6214982968adc219