Back to Search
Start Over
Rapid Characterization and Identification of Chemical Constituents in Gentiana radix before and after Wine-Processed by UHPLC-LTQ-Orbitrap MSn
- Source :
- Molecules, Volume 23, Issue 12, Molecules, Vol 23, Iss 12, p 3222 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Gentiana radix is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed . Based on the accurate mass measurement (&lt<br />5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from Gentiana radix, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of . By comparing the structure and peak areas of chemical constituents in crude and wine-processed Gentiana radix, we found that some compounds in crude and wine-processed Gentiana radix were significantly different.
- Subjects :
- iridoids
Gentiana radix
Pharmaceutical Science
wine-processing
Mass spectrometry
Orbitrap
01 natural sciences
Analytical Chemistry
law.invention
lcsh:QD241-441
Triterpenoid
lcsh:Organic chemistry
law
Drug Discovery
Physical and Theoretical Chemistry
UHPLC-LTQ-Orbitrap
xanthones
Wine
Chromatography
biology
010405 organic chemistry
Chemistry
010401 analytical chemistry
Organic Chemistry
biology.organism_classification
Mass measurement
0104 chemical sciences
Highly sensitive
Chemistry (miscellaneous)
Chemical constituents
flavonoids
characteristic fragmentation pathways
Molecular Medicine
Gentiana
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....bac26f687b941d730c9adcac5d84028b
- Full Text :
- https://doi.org/10.3390/molecules23123222