50 results on '"Huitao Zhang"'
Search Results
2. Associations of the circulating levels of cytokines with the risk of myeloproliferative neoplasms: a bidirectional mendelian-randomization study
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Hao Xiong, Huitao Zhang, Jun Bai, Yanhong Li, Lijuan Li, and Liansheng Zhang
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Myeloproliferative neoplasms ,Mendelian randomization ,Inflammatory ,Cytokines ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Objective In the pathogenesis of myeloproliferative neoplasms (MPN), inflammation plays an important role. However, it is unclear whether there is a causal link between inflammation and MPNs. We used a bidirectional, two-sample Mendelian randomization (MR) approach to investigate the causal relationship between systemic inflammatory cytokines and myeloproliferative neoplasms. Methods A genome-wide association study (GWAS) of 8293 European participants identified genetic instrumental variables for circulating cytokines and growth factors. Summary statistics of MPN were obtained from a GWAS including 1086 cases and 407,155 controls of European ancestry. The inverse-variance-weighted method was mainly used to compute odds ratios (OR) and 95% confidence intervals (Cl). Results Our results showed that higher Interleukin-2 receptor, alpha subunit (IL-2rα) levels, and higher Interferon gamma-induced protein 10 (IP-10) levels were associated with an increased risk of MPN (OR = 1.36,95%CI = 1.03–1.81, P = 0.032; OR = 1.55,95%CI = 1.09–2.22, P = 0.015; respectively).In addition, Genetically predicted MPN promotes expression of the inflammatory cytokines interleukin-10 (IL-10) (BETA = 0.033, 95% CI = 0.003 ~ 0.064, P = 0.032) and monokine induced by interferon-gamma (MIG) (BETA = 0.052, 95% CI = 0.002–0.102, P = 0.043) and, on activation, normal T cells express and secrete RANTES (BETA = 0.055, 95% CI = 0.0090.1, P = 0.018). Conclusion Our findings suggest that cytokines are essential to the pathophysiology of MPN. More research is required if these biomarkers can be used to prevent and treat MPN.
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- 2024
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3. Grating-based x-ray dark-field CT for lung cancer diagnosis in mice
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Peiyuan Guo, Li Zhang, Jincheng Lu, Huitao Zhang, Xiaohua Zhu, Chengpeng Wu, Xiangwen Zhan, Hongxia Yin, Zhenchang Wang, Yan Xu, and Zhentian Wang
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Disease progression ,Early detection of cancer ,Lung diseases ,Tomography (x-ray computed) ,X-rays ,Medical physics. Medical radiology. Nuclear medicine ,R895-920 - Abstract
Abstract Background The low absorption of x-rays in lung tissue and the poor resolution of conventional computed tomography (CT) limits its use to detect lung disease. However, x-ray dark-field imaging can sense the scattered x-rays deflected by the structures being imaged. This technique can facilitate the detection of small alveolar lesions that would be difficult to detect with conventional CT. Therefore, it may provide an alternative imaging modality to diagnose lung disease at an early stage. Methods Eight mice were inoculated with lung cancers simultaneously. Each time two mice were scanned using a grating-based dark-field CT on days 4, 8, 12, and 16 after the introduction of the cancer cells. The detectability index was calculated between nodules and healthy parenchyma for both attenuation and dark-field modalities. High-resolution micro-CT and pathological examinations were used to crosscheck and validate our results. Paired t-test was used for comparing the ability of dark-field and attenuation modalities in pulmonary nodule detection. Results The nodules were shown as a signal decrease in the dark-field modality and a signal increase in the attenuation modality. The number of nodules increased from day 8 to day 16, indicating disease progression. The detectability indices of dark-field modality were higher than those of attenuation modality (p = 0.025). Conclusions Compared with the standard attenuation CT, the dark-field CT improved the detection of lung nodules. Relevance statement Dark-field CT has a higher detectability index than conventional attenuation CT in lung nodule detection. This technique could improve the early diagnosis of lung cancer. Key points • Lung cancer progression was observed using x-ray dark-field CT. • Dark-field modality complements with attenuation modality in lung nodule detection. • Dark-field modality showed a detectability index higher than that attenuation in nodule detection. Graphical Abstract
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- 2024
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4. A spider mating plug functions to protect sperm.
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He Jiang, Yongjia Zhan, Qingqing Wu, Huitao Zhang, Matjaž Kuntner, and Lihong Tu
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Medicine ,Science - Abstract
Mating plugs in animals are ubiquitous and are commonly interpreted to be products of mating strategies. In spiders, however, mating plugs may also take on functions beyond female remating prevention. Due to the vagaries of female genital (spermathecal) anatomy, most spiders face the problem of having to secure additional, non-anatomical, protection for transferred sperm. Here, we test the hypothesis that mating plugs, rather than (or in addition to) being adaptations for mating strategies, may serve as sperm protection mechanism. Based on a comparative study on 411 epigyna sampled from 36 families, 187 genera, 330 species of entelegyne spiders, our results confirm the necessity of a sperm protection mechanism. We divided the entelegyne spermathecae into four types: SEG, SED, SCG and SCD. We also studied detailed morphology of epigynal tracts in the spider Diphya wulingensis having the SEG type spermathecae, using 3D-reconstruction based on semi thin histological series section. In this species, we hypothesize that two distinct types of mating plug, the sperm plug and the secretion plug, serve different functions. Morphological details support this: sperm plugs are formed on a modified spermathecal wall by the spilled sperm, and function as a temporary protection mechanism to prevent sperm from leaking and desiccating, while secretion plugs function in postcopulation both as a permanent protection mechanism, and to prevent additional mating. Furthermore, with the modified spermathecal wall of S2 stalk, the problem of shunt of sperm input and output, and the possibility of female multiple mating have been resolved. Variation in spermathecal morphology also suggests that the problem of sperm protection might be resolved in different ways in spiders. Considering mating plugs of varying shapes and origins in the vast morphospace of spiders, we conclude that mating plugs might serve different purposes that relate both to mating strategies, as well as to sperm protection.
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- 2024
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5. Fracture-vuggy carbonate reservoir characterization based on multiple geological information fusion
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Yan Wang, Pengfei Xie, Huitao Zhang, Yuming Liu, and Aiying Yang
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carbonate reservoir ,Fractured-vuggy reservoir ,geological modeling ,probability fusion ,fracture ,Science - Abstract
The complexity and strong heterogeneity of carbonate reservoirs with fracture-vuggy structures present significant challenges in reservoir characterization. To address these challenges, we propose a novel multi-element information fusion modeling approach. This approach is designed to integrate multiple methods and incorporate multi-probability fusion at various facies and scales, thereby bridging the gap between geological information and reservoir modeling. Our methodology involves four key steps. First, the statistics between frequency of karst and geological information are acquired, and we quantify the statistics to regression equations. Second, these regression equations are transferred to probability bodies. The probability bodies can be applied in modeling as a soft control. But just one single body can be input in modeling process. Third, multiple probability bodies are fused into a fusion probability body by a probability fusion algorithm, which can keep the potential information of probability bodies. Finally, we apply the probability body in modeling workflow. By this way, the fusion method bridges the gap between geological information and modeling. The model established through our proposed method showed a significant level of consistency with reservoir re-evaluation, achieving an impressive 90% degree of alignment. Furthermore, the history match analysis revealed a high correlation, indicating the model's reliability. The method effectively integrates various scales and types of geological information, offering an accurate approach to complex carbonate reservoir modeling.
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- 2024
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6. Mechanical stimulation on a microfluidic device to highly enhance small extracellular vesicle secretion of mesenchymal stem cells
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Rui Hao, Shi Hu, Huitao Zhang, Xi Chen, Zitong Yu, Jingyi Ren, Hang Guo, and Hui Yang
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Microfluidics ,Small extracellular vesicles ,Mechanical squeezing ,Secretion amount ,Mesenchymal stem cells ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Small extracellular vesicles (sEVs) are recognized as promising detection biomarkers and attractive delivery vehicles, showing great potential in diagnosis and treatment of diseases. However, the applications of sEVs are usually restricted by their poor secretion amount from donor cells under routine cell culture conditions, which is especially true for mesenchymal stem cells (MSCs) due to their limited expansion and early senescence. Here, a microfluidic device is proposed for boosting sEV secretion from MSCs derived from human fetal bone marrow (BM-MSCs). As the cells rapidly pass through a microfluidic channel with a series of narrow squeezing ridges, mechanical stimulation permeabilizes the cell membrane, thus promoting them to secrete more sEVs into extracellular space. In this study, the microfluidic device demonstrates that mechanical-squeezing effect could increase the secretion amount of sEVs from the BM-MSCs by approximately 4-fold, while maintaining cellular growth state of the stem cells. Further, the secreted sEVs are efficiently taken up by immortalized human corneal epithelial cells and accelerate corneal epithelial wound healing in vitro, indicating that this technique wound not affect the functionality of sEVs and demonstrating the application potentials of this technique.
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- 2023
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7. Optimization of Protoplast Preparation System from Leaves and Establishment of a Transient Transformation System in Apium graveolens
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Jiageng Du, Huitao Zhang, Weilong Li, Xiaoyan Li, Zhuo Wang, Ying Zhang, Aisheng Xiong, and Mengyao Li
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celery ,protoplast preparation ,transient transformation system ,subcellular localization ,Agriculture - Abstract
Protoplast culture and transformation technology offer a novel method for developing new plant varieties. Nonetheless, the effective preparation of protoplasts and transformation technology specific to celery has yet to be achieved. This study utilized celery seedling leaves as the primary materials to examine the key factors influencing protoplast isolation. The aim was to prepare leaf protoplasts with a high yield and of high quality and subsequently conduct transient gene transformation and expression. The findings indicated that the most effective procedure for isolating and purifying protoplasts was enzymatic digestion using an enzyme solution consisting of 2.0% cellulase, 0.1% pectolase, and 0.6 M mannitol for a duration of 8 h. Subsequently, the protoplasts were filtered through a 400-mesh sieve and purified through centrifugation at 200× g. Within this system, the overall protoplast yield was exceptionally high, reaching a viability rate of up to 95%. The transient transformation system yielded a maximum transformation efficiency of approximately 53%, as evaluated using the green fluorescent protein (GFP) as a reporter gene. The parameters of the transient transformation system were as follows: a protoplast concentration of 5 × 105 cells·mL−1, exogenous DNA concentration of 500 μg·mL−1, final concentration of PEG4000 at 40%, and transformation duration of 15 min. The transient transformation system was also utilized to further analyze the protein localization characteristics of the celery transcription factor AgMYB80. The findings indicated that AgMYB80 predominantly localizes in the nucleus, thereby confirming the reliability and effectiveness of the transient transformation system. This study successfully established an efficient system for isolating, purifying, and transforming celery protoplasts, and will serve as a basis for future studies on molecular biology and gene function.
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- 2023
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8. Chattering suppression of the sliding mode observer for marine electric propulsion motor based on piecewise power function
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Xiangfeng Li, Shengqiang Zhan, Fang Guo, Zidan Zhuang, Huitao Zhang, Hui Liao, and Lili Qu
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PMSM ,sliding mode observer ,sensorless control ,piecewise power function ,chatter suppression ,General Works - Abstract
The sliding mode observer (SMO)–based sensorless control is essentially a discontinuous switching control algorithm. Therefore, there is large speed or torque chattering in the permanent magnet synchronous motor (PMSM) with existing sliding mode observer–based control methods. In order to solve this problem well, an algorithm based on an improved sliding mode observer is proposed. In detail, the piecewise power function approach is used to replace the traditional ones, that is, the sgn function or the sigmoid function approach. Simulation verifications are conducted to validate the effectiveness of the proposed observer. It shows that the piecewise power function–based sliding mode observer algorithm has obvious advantages in the stability of the three-phase current, namely, it improves the estimation accuracy of the rotor position, rotor speed, and dynamic response to various loads. For further verification, the proposed sliding mode observer algorithm is implemented and verified in a marine electric propulsion motor with a DSP chip of TMS320F28377D. Experimental results validate that the piecewise power function–based sliding mode observer algorithm has high control accuracy and fewer fluctuations, which are all vital in marine engineering.
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- 2022
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9. Generation of three lines from multiorgan venous and lymphatic defect syndrome patients
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Xiaojun Hu, Junjie Mao, Ke Zhang, Huitao Zhang, Dan Li, Bin Zhou, Hong Shan, Bing Li, and Pengfei Pang
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Biology (General) ,QH301-705.5 - Abstract
Multiorgan venous and lymphatic defect (MOVLD) syndrome is a unique visceral vascular malformations with complex etiologies. In this study, primary skin fibroblasts were obtained from three MOVLD patients and reprogrammed into iPSCs by Yamanaka’s classical strategy. The MOVLD- iPSCs carrying the DDX24 p.Glu271Lys mutation were confirmed by Sanger sequencing. The pluripotency of MOVLD-iPSCs was verified by the specific molecular markers and gene expression, trilineage differentiation potential. The establishment of the MOVLD-iPSCs will provide a useful model for understanding the mechanisms involved the MOVLD and promoting the development of medical treatment.
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- 2022
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10. A finite element analysis of the impact of split pole shoes on magnetic liquid sealing performance
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Zhiguo Liu, Huitao Zhang, and Li Yang
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split magnetic liquid sealing ,split pole shoe ,magnetic sealant ,sealing performance ,finite element analysis ,Mathematics ,QA1-939 - Abstract
Though split pole shoe can solve the problems of increased cost and decreased efficiency caused by replacing pole shoes in the test, assembling and use of split sealing devices, it fails to overcome the issue of sealing in the gap between split pole shoe's junction surface and shaft. In this paper, a split magnetic liquid sealing structure is designed, combining magnetic liquid sealing and split pole shoe's plane sealing. In our study, we have adopted three methods for split sealing and use theory and simulation to ascertain the sealing performance of the split magnetic liquid sealing structure designed by us. The results indicate that magnetic sealant bonded split pole shoes show better sealing performance as compared to those bonded by ordinary sealant, and that the structural optimisation results can meet the requirement for sealing performance. The simulation results coincide with theoretical results, which proves the correctness of this research.
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- 2021
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11. Multi-Scale Dilated Convolution Neural Network for Image Artifact Correction of Limited-Angle Tomography
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Haichuan Zhou, Yining Zhu, Qian Wang, Jinqiu Xu, Ge Li, Defeng Chen, Yingying Dong, and Huitao Zhang
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Limited-angle tomography ,artifact correction ,multi-scale ,dilated convolution ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
Limited-angle computed tomography (CT) has arisen in some medical and industrial applications. It is also a challenging problem since some scan views are missing and the directly reconstructed images often suffer from severe distortions. For such kind of problems, we analyze the features of limited-angle CT images and propose a multi-scale dilated convolution neural network (MSD-CNN) to correct the artifacts and to restore the image. In this network, the dilated convolution layer and multi-scale pooling layer are combined to form a group and exited in the whole encoder-decoder process. Since the dilated convolutions support an exponential expansion of the receptive field without losing resolution and coverage, the obtained artifact features possess the multi-scale characteristic. Furthermore, to improve the effectiveness and accuracy of the training step, we employ a preprocessing method, which extracts image patches. Numerical experiments verify the out-performance of the proposed method compared with some conventional methods, such as Unet based deep learning,TV- and L0 -based optimization methods.
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- 2020
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12. CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
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Yanli Liu, Xuejing Li, Bin Zhang, Ye Fu, Aimin Yang, Hongjie Zhang, Huitao Zhang, Yingying Niu, Jisheng Nie, and Jin Yang
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Oxidative DNA damage ,Polycyclic aromatic hydrocarbons ,Smoking ,Cytochrome P4501A1 ,DNA methylation ,Industrial medicine. Industrial hygiene ,RC963-969 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Multiple factors, including co-exposure between lifestyle and environmental risks, are important in susceptibility to oxidative DNA damage. However, the underlying mechanism is not fully understood. This study was undertaken to evaluate whether Cytochrome P4501A1 (CYP1A1) methylation can mediate the co-exposure effect between smoking and occupational polycyclic aromatic hydrocarbons (PAH) in development of oxidative DNA damage. Methods We explored the associations between smoking and occupational PAH co-exposure effect, CYP1A1 methylation and oxidative DNA damage among 500 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (1-hydroxypyrene, 1-OHP; 2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; and 9-hydroxyphenanthren, 9-PHE) and a marker of oxidative DNA damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. CYP1A1 methylation was measured by pyrosequencing. Finally, mediation analysis was performed to investigate whether CYP1A1 methylation mediated smoking and occupational PAH co-exposure effect on oxidative DNA damage. Results We observed significant associations of smoking and 1-OHP co-exposure with CYP1A1 hypomethylation (OR: 1.87, 95% CI: 1.01–3.47) and high 8-OHdG (OR: 2.13, 95% CI: 1.14–3.97). There was a significant relationship between CYP1A1 hypomethylation and high 8-OHdG (1st vs. 3rd tertile = 1.58, 95% CI: 1.01–2.47, P for trend = 0.046). In addition, mediation analysis suggested CYP1A1 hypomethylation could explain 13.6% of effect of high 8-OHdG related to smoking and 1-OHP co-exposure. Conclusions Our findings suggested that the co-exposure effect of smoking and occupational PAH could increase the risk of oxidative DNA damage by a mechanism partly involving CYP1A1 hypomethylation.
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- 2019
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13. A method for material decomposition and quantification with grating based phase CT.
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Shiwo Deng, Yining Zhu, Huitao Zhang, Qian Wang, Peiping Zhu, Kai Zhang, and Peng Zhang
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Medicine ,Science - Abstract
Material decomposition (MD) is an important application of computer tomography (CT). For phase contrast imaging, conventional MD methods are categorized into two types with respect to different operation sequences, i.e., "before" or "after" image reconstruction. Both categories come down to two-step methods, which have the problem of noise amplification. In this study, we incorporate both phase and absorption (PA) information into MD process, and correspondingly develop a simultaneous algebraic reconstruction technique (SART). The proposed method is referred to as phase & absorption material decomposition-SART (PAMD-SART). By iteratively solving an optimization problem, material composition and substance quantification are reconstructed directly from absorption and differential phase projections. Comparing with two-step MD, the proposed one-step method is superior in noise suppression and accurate decomposition. Numerical simulations and synchrotron radiation based experiments show that PAMD-SART outperforms the classical MD method (image-based and dual-energy CT iterative method), especially for the quantitative accuracy of material equivalent atomic number.
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- 2021
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14. Design and Performance Simulation of Computer Control System for Automatic Monitoring of Upper Computer Communication Operation State
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Li Yang and Huitao Zhang
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Mechanical engineering and machinery ,TJ1-1570 - Abstract
The upper computer communication operation state automatic monitoring system is mainly used to remotely monitor the equipment, obtain various parameter indexes in the operation process of remote equipment, realize remote monitoring and fault diagnosis, and improve the management efficiency of decentralized equipment. This paper completes the design of communication, data storage, query, and other subsystems of upper and lower computers. The lower computer establishes a data channel with the OPC server through the MPI protocol and uploads the collected data to the OPC server in real time. The upper computer reads the data through the OPC server and displays the changes of monitored parameters in real time through the monitoring interface, so as to give an alarm under abnormal conditions. In addition, since the default database of Kingview is access, considering that the Microsoft Access database can store up to 2G of content, in order to upgrade and expand the subsequent system, SQL Server database is selected for data query, backup, and saving. The parameter setting method of communication control system is analyzed, the simulation model of industrial boiler control system is established by using Matlab/Simulink, and the interface between host computer software (IBCCS-e) and the model is provided. This paper analyzes the results of communication parameter adjustment. The simulation results show that the industrial boiler computer control system (IBCCS) has stable performance, low cost, convenient operation, and good maintainability. After further improvement, it has certain application value in the operation transformation of new small- and medium-sized boilers and original boilers.
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- 2021
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15. Comparative morphology refines the conventional model of spider reproduction.
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Yongjia Zhan, He Jiang, Qingqing Wu, Huitao Zhang, Zishang Bai, Matjaž Kuntner, and Lihong Tu
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Medicine ,Science - Abstract
Our understanding of spider reproductive biology is hampered by the vast anatomical diversity and difficulties associated with its study. Although authors agree on the two general types of female spider genitalia, haplogyne (plesiomorphic) and entelegyne (apomorphic), our understanding of variation within each group mostly concerns the external genital part, while the internal connections with the reproductive duct are largely unknown. Conventionally and simplistically, the spermathecae of haplogynes have simple two-way ducts, and those of entelegynes have separate copulatory and fertilization ducts for sperm to be transferred in and out of spermathecae, respectively. Sperm is discharged from the spermathecae directly into the uterus externus (a distal extension of the oviduct), which, commonly thought as homologous in both groups, is the purported location of internal fertilization in spiders. However, the structural evolution from haplo- to entelegyny remains unresolved, and thus the precise fertilization site in entelegynes is ambiguous. We aim to clarify this anatomical problem through a widely comparative morphological study of internal female genital system in entelegynes. Our survey of 147 epigyna (121 examined species in 97 genera, 34 families) surprisingly finds no direct connection between the fertilization ducts and the uterus externus, which, based on the homology with basal-most spider lineages, is a dead-end caecum in entelegynes. Instead, fertilization ducts usually connect with a secondary uterus externus, a novel feature taking over the functional role of the plesiomorphic uterus externus. We hypothesize that the transition from haplo- to entelegyny entailed not only the emergence of the two separate duct systems (copulatory, fertilization), but also involved substantial morphological changes in the distal part of the oviduct. Thus, the common oviduct may have shifted its distal connection from the uterus externus to the secondary uterus externus, perhaps facilitating discharge of larger eggs. Our findings suggest that the conventional model of entelegyne reproduction needs redefinition.
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- 2019
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16. Hemodialysis Arteriovenous Fistula Dysfunction: Retrospective Comparison of Post-thrombotic Percutaneous Endovascular Interventions with Pre-emptive Angioplasty
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Xinyan Hu, Bing Li, Junjie Mao, Xiaojun Hu, Jingjing Zhang, Hui Guo, Dashuai Wang, Yongyu Zhang, Jianan He, Ni Zhao, Huitao Zhang, and Pengfei Pang
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Angioplasty ,Graft Occlusion, Vascular ,Thrombosis ,General Medicine ,Urokinase-Type Plasminogen Activator ,Arteriovenous Shunt, Surgical ,Treatment Outcome ,Renal Dialysis ,Arteriovenous Fistula ,Humans ,Surgery ,Cardiology and Cardiovascular Medicine ,Angioplasty, Balloon ,Vascular Patency ,Retrospective Studies - Abstract
We aimed to compare the clinical outcomes of pre-emptive angioplasty versus post-thrombotic percutaneous endovascular restoration of dysfunctional arteriovenous fistula (AVF).This retrospective study reviewed the data from 80 patients who underwent 114 endovascular interventions for a malfunctioning AVF from July 2016 to August 2019. Stenotic AVFs were treated with pre-emptive angioplasty. Thrombosed AVFs were treated with percutaneous pharmacomechanical fibrinolysis with urokinase used only during the operation or continuously infused. The differences in patency rates were evaluated using the Kaplan-Meier method. In addition, univariate and multivariate regression Cox models were used to determine influential factors on the postintervention primary patency.Post-thrombotic interventions and pre-emptive angioplasty yielded statistically similar rates in clinical success (100 vs. 100%), anatomic success (94 vs. 89%; P = 0.52), complication (4 vs. 11%; P = 0.29), as well as postintervention primary, assisted primary and secondary patency (P = 0.80; 0.57; 0.57). The use of pre-emptive angioplasty was associated with reduced total cost (¥25,108 vs. ¥30,833, P 0.001). The patients who used urokinase only during the operation prolonged both the primary and assisted primary patency (P = 0.02; 0.002), while those with continuous infusion of urokinase had worst patency rates and high costs (¥39,275 vs. ¥25,108 vs. ¥27,140, P 0.001). Compared with the other locations, dysfunction in the anastomotic or juxta-anastomotic segment (HR = 0.41, P = 0.001) was associated with prolonged postintervention primary patency.No clinical outcome differences were found between the post-thrombotic percutaneous endovascular interventions and pre-emptive angioplasty. However, pre-emptive angioplasty decreased access expenditure.
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- 2022
17. Effects of bacterial inoculation on lignocellulose degradation and microbial properties during cow dung composting.
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Liuyan Zhou, Xinping Yang, Xiaowu Wang, Lei Feng, Zhifang Wang, Jinping Dai, Huitao Zhang, and Yuqing Xie
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- 2023
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18. Reconstruction Algorithm-Based CT Imaging for the Diagnosis of Hepatic Ascites
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Huitao Zhang, Wenhao Lv, Haofeng Diao, and Li Shang
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Article Subject ,General Immunology and Microbiology ,Applied Mathematics ,Ascites ,Water ,General Medicine ,General Biochemistry, Genetics and Molecular Biology ,Artificial Intelligence ,Modeling and Simulation ,Humans ,Radiographic Image Interpretation, Computer-Assisted ,Calcium ,Tomography, X-Ray Computed ,Algorithms ,Iodine - Abstract
The study was aimed at exploring the diagnostic value of artificial intelligence reconstruction algorithm combined with CT image parameters on hepatic ascites, expected to provide a reference for the etiological evaluation of clinical abdominal effusion. Specifically, the adaptive iterative hard threshold (AIHT) algorithm for CT image reconstruction was proposed. Then, 100 patients with peritoneal effusion were selected as the research subjects. After 8 cases were excluded, the remaining was divided into 50 cases of the S1 group (hepatic ascites) and 42 cases of the D0 group (cancerous peritoneal effusion). Gemstone energy spectrum CT scanning was performed on all patients, and CT image parameters of the two groups were compared. It was found that CT value of mixed energy, CT value of 60-100 KeV single energy, concentration value of water (calcium), concentration value of water (iodine), and slope of energy spectrum curve in the S1 group were significantly lower than those in the D0 group ( P < 0.05 ). The effective atomic number in the S1 group was significantly higher than that in the D0 group ( P < 0.05 ). Of the 50 patients in the S1 group, 3 (6%) had an ascending and 47 (94%) had a descending spectral curve. Of the 42 patients in the D0 group, 37 (88.1%) had an ascending and 5 (11.9%) had a descending spectral curve. The sensitivity and specificity of water (iodine) were 0.927 and 0.836, respectively. The sensitivity and specificity of water (calcium) were 0.863 and 0.887, respectively. For different scan ranges ([0,90]; [0,120]), root mean square error (RMSE) of AIHT reconstructed image was significantly smaller than that of traditional algorithm, while peak signal-to-noise ratio (PSNR) was opposite. The differences were statistically significant ( P < 0.05 ). In conclusion, AIHT-based CT images can better display the distribution of hepatic ascites, and the parameters of CT value, effective atomic number, water (iodine), water (calcium), and spectral curve can all provide help for the identification of hepatic ascites. Especially, water (iodine) and water (calcium) demonstrated high diagnostic performance of hepatic ascites.
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- 2022
19. Protein Post-translational Modification Site Prediction using Deep Learning
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Yujuan Deng, Yunfang Fu, Huitao Zhang, Xin Liu, and Zhiguo Liu
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General Earth and Planetary Sciences ,General Environmental Science - Published
- 2022
20. Encapsulating has-miR-29a into HuMSCs-derived Exosomes by a Nanoengineering Platform to Efficiently Alleviate Aβ1-42 Expression in APPswe-SH-SY5Y Cells
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Huitao Zhang, Hui Yang, Rui Hao, Zitong Yu, Shi Hu, Jingyi Ren, Juan Ding, and Yi Zhang
- Abstract
Background Exosomes derived from human umbilical cord mesenchymal stem cells (HuMSCs) have been utilized as a potential therapeutic candidate to alleviate amyloid β-protein (Aβ) expression in APP/PS1 or 5XFAD transgenic mice due to their low immunogenicity and extensive sources. Accumulated evidence also suggests that encysting appropriate cargos into exosomes can more effectively reduce Aβ expression by interfering with the metabolism of β-Amyloid precursor protein (APP). Therefore, exosomes loaded with bioactive substances to reduce Aβ expression have attracted much attention. A new strategy to load therapeutic exogenous cargoes of interest into exosomes in a high-throughput and straightforward fashion is desired. Results In this study, neuroblastoma SH-SY5Y cells were stably transfected with Swedish mutant APP695K, 595N plasmid to construct a cell model of Aβ overexpression. Then extracted Exosomes from HuMSCs and loaded with micro (miR)-29a via a microfluidic exosome nanoengineering platform (ExoNP) to target a post-transcriptional modification of the β-secretase1 (β-site APP cleaving enzyme 1, BACE1) gene to prevent protein expression. Finally, processed exosomes were utilized to treat APPswe-SH-SY5Y cells and decrease the expression of Aβ1−42 and BACE1 and further alleviated apoptosis. Conclusion HuMSCs-derived exosomes loaded with hsa-miR-29a via ExoNP to efficiently deliver to APPswe-SH-SY5Y cells and alleviate Aβ1−42 expression and promote cell survival.
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- 2022
21. One-Step Method for Material Quantitation Using In-Line Tomography With Single Scanning
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Suyu Liao, Shiwo Deng, Yining Zhu, Huitao Zhang, Peiping Zhu, Kai Zhang, and Xing Zhao
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Image and Video Processing (eess.IV) ,FOS: Electrical engineering, electronic engineering, information engineering ,Biomedical Engineering ,FOS: Physical sciences ,Medical Physics (physics.med-ph) ,Electrical Engineering and Systems Science - Image and Video Processing ,Physics - Medical Physics - Abstract
Objective: Quantitative technique based on In-line phase-contrast computed tomography with single scanning attracts more attention in application due to the flexibility of the implementation. However, the quantitative results usually suffer from artifacts and noise, since the phase retrieval and reconstruction are independent ("two-steps") without feedback from the original data. Our goal is to develop a method for material quantitative imaging based on a priori information specifically for the single-scanning data. Method: An iterative method that directly reconstructs the refractive index decrement delta and imaginary beta of the object from observed data ("one-step") within single object-to-detector distance (ODD) scanning. Simultaneously, high-quality quantitative reconstruction results are obtained by using a linear approximation that achieves material decomposition in the iterative process. Results: By comparing the equivalent atomic number of the material decomposition results in experiments, the accuracy of the proposed method is greater than 97.2%. Conclusion: The quantitative reconstruction and decomposition results are effectively improved, and there are feedback and corrections during the iteration, which effectively reduce the impact of noise and errors. Significance: This algorithm has the potential for quantitative imaging research, especially for imaging live samples and human breast preclinical studies.
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- 2022
22. The effect of bacteria inoculation on the lignocellulose degradation and the microbial properties during cow dung composting
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Liuyan Zhou, Xinping Yang, Yuqing Xie, Xiaowu Wang, Jinping Dai, Zhifang Wang, Huitao Zhang, and Lei Feng
- Abstract
The goal of this work was to explore the potential effects of bacteria inoculation on the lignocellulose degradation and the underlying microbial mechanism during cow dung composting. The results suggested that bacteria inoculation better-accelerated temperature rose and increased thermophilic phase. Compared to the control (without inoculation, CK), the cellulose, hemicellulose, and lignin degradation rates of treated group (EG) increased from 53.3% to 70.0%, 50.2% to 61.3% and 46.4% to 60.0%, respectively. The microbial community structure and diversity were obviously changed by inoculation. Moreover, inoculation contributed to modulate the key compost microbial functional populations linking to the degradation of lignocelluloses by stamp analysis. Correlation matrix analysis indicated that the expression of bacterial lingo-cellulolytic enzymes were closely related to the key microbial functional populations. Overall, the results confirmed the importance of bacteria inoculation and have important implication for promoting the efficiency and quality of cow dung compost.
- Published
- 2022
23. MALDI-MS-based biomarker analysis of extracellular vesicles from human lung carcinoma cells
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Chao Zhao, Wei Li, Zitong Yu, Qian Luo, Renjie Zhang, Huitao Zhang, Hui Yang, and Shi Hu
- Subjects
0303 health sciences ,biology ,Cell growth ,Chemistry ,General Chemical Engineering ,010401 analytical chemistry ,S100A10 ,General Chemistry ,medicine.disease ,01 natural sciences ,RPS27A ,0104 chemical sciences ,Ribosome assembly ,03 medical and health sciences ,Cell culture ,Cancer cell ,biology.protein ,medicine ,Cancer research ,Lung cancer ,Fetal bovine serum ,030304 developmental biology - Abstract
Extracellular vesicles (EVs) are actively secreted by mammalian cells. They are increasingly recognized as promising circulating biomarkers of disease progression. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is currently one of the most powerful techniques for the rapid analysis of biological samples, especially for discovering biomarkers for disease diagnosis and prognosis. It is unclear what cell culture medium components and EV isolation methods are suitable for MALDI-TOF MS analysis. Using a human lung carcinoma cell line (A549), we investigated and optimized the critical experimental conditions for EVs' protein profiling by combining differential ultracentrifugation and MALDI-TOF MS. The results demonstrated that medium components and ultracentrifugation procedures to extract EVs played important roles in MS detection. Compared with EV-depleted serum and normal serum medium, conditioned medium with 2% fetal bovine serum in this study maintained cell proliferation and displayed significant protein profiling of EVs. RPS27A (ribosomal protein), which plays an essential role in mRNA translation and ribosome assembly for the differentiation of cancer cells, was detected from the EVs of lung cancer cells associated with cancer cell migration and invasion. We also found the known tumor diagnosis marker, which is S100A10_S100 calcium-binding protein A10. Therefore, MALDI-TOF MS-based EV analysis with optimized experimental protocols can contribute to future development of rapid screening techniques of protein biomarkers associated with early cancer diagnosis.
- Published
- 2021
24. Design of Anti-Lock Braking System for FSAE Racing Vehicle Based on New Slip Ratio Observation Method
- Author
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Yicai Liu, Da Wang, Luxu Liang, Huitao Zhang, Wenjie Wang, Mo Lv, Yiyang Wang, Yi Sun, and Shuai Yang
- Published
- 2022
25. Effect of SARS-CoV-2 Infection on the Microbial Composition of Upper Airway
- Author
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Changli Tu, Xiaobin Zheng, Huitao Zhang, Jing Liu, Pengfei Pang, Yingjian Liang, Zhenguo Wang, Yiming Wang, Xiaojun Hu, Hong Shan, and Zhonghe Li
- Subjects
0301 basic medicine ,Pharmacology ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Opportunistic infection ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,030106 microbiology ,Microbial composition ,macromolecular substances ,Biology ,medicine.disease ,medicine.disease_cause ,biology.organism_classification ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,medicine ,Pharmacology (medical) ,030212 general & internal medicine ,Airway ,Bacteria ,Coronavirus - Abstract
Methods Forty-four COVID-19 patients (severe/critical: N = 8, non-severe: N = 36) were examined by next generation sequencing (NGS) of nasopharyngeal test paper to observe the effect of novel coronavirus infection to the microbial composition in upper airway. Results In these nasopharyngeal test paper samples, 38 kinds of bacteria, 10 kinds of viruses except SARS-CoV-2, nine kinds of fungi and three kinds of atypical pathogens had been found. There was some difference in microbial composition in the upper airway between severe and non-severe cases. Summary These results are important for us to study the effect of SARS-CoV-2 on the local microbial composition of upper airway and prevent opportunistic infection in severe patients.
- Published
- 2020
26. Synthesis of MnO2 modified porous carbon spheres by preoxidation-assisted impregnation for catalytic oxidation of indoor formaldehyde
- Author
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Changming Zhang, Caimei Fan, Huitao Zhang, Rui Li, Yaqi Wang, Xiaochao Zhang, and Song Wen
- Subjects
Materials science ,Scanning electron microscope ,Mechanical Engineering ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Catalysis ,Adsorption ,X-ray photoelectron spectroscopy ,Chemical engineering ,Catalytic oxidation ,Mechanics of Materials ,Desorption ,General Materials Science ,Fourier transform infrared spectroscopy ,Temperature-programmed reduction ,0210 nano-technology - Abstract
Resin-based porous carbon spheres with well dispersed MnO2 particles were sucessfully synthesised by steam activation and preoxidation-assisted impregnation of manganese nitrate salt. X-ray diffractometer (XRD), Fourier Transform Infrared spectroscopy (FT-IR), N2 adsorption–desorption, scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), H2 temperature programmed reduction (H2-TPR), O2 temperature-programmed desorption (O2-TPD) and in-situ diffuse reflectance fourier transform spectrometry (DRIFTS) were applied to characterize the obtained porous carbon spheres, and the HCHO adsorption and stability performances were evaluated in a fixed bed reactor. The results demonstrated that surface chemical properties and HCHO removal performances were significantly enhanced after the modification of preoxidation-assisted impregnation. The optimal manganese nitrate loading value, and relative humidity were 6 and 50%, respectively. Moreover, the higher HCHO concentration showed a smaller breakthrough time. The HCHO removal efficiency of ACS–O-6% Mn remained 100% even after reaction for 20 h at room temperature, while the average HCHO removal efficiency only declines by 0.2% compared with the first adsorption after 10 regeneration cycles. The in-situ DRIFTS results showed the smaller accumulation and faster desorption of intermediate products over the ACS–O-6% Mn. The HCHO removal mechanism analysis indicated that the enriched Mn3+ and surface active chemisorbed oxygen accounted for the excellent catalytic oxidation activity and stability.
- Published
- 2020
27. Multi-Scale Dilated Convolution Neural Network for Image Artifact Correction of Limited-Angle Tomography
- Author
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Yining Zhu, Huitao Zhang, Yingying Dong, Jinqiu Xu, Qian Wang, Haichuan Zhou, Ge Li, and Defeng Chen
- Subjects
0209 industrial biotechnology ,General Computer Science ,Computer science ,multi-scale ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Scale (descriptive set theory) ,artifact correction ,02 engineering and technology ,Convolutional neural network ,Image (mathematics) ,Convolution ,020901 industrial engineering & automation ,0202 electrical engineering, electronic engineering, information engineering ,Preprocessor ,General Materials Science ,Computer vision ,Artifact (error) ,business.industry ,Deep learning ,General Engineering ,Process (computing) ,dilated convolution ,Limited-angle tomography ,020201 artificial intelligence & image processing ,Artificial intelligence ,lcsh:Electrical engineering. Electronics. Nuclear engineering ,business ,lcsh:TK1-9971 - Abstract
Limited-angle computed tomography (CT) has arisen in some medical and industrial applications. It is also a challenging problem since some scan views are missing and the directly reconstructed images often suffer from severe distortions. For such kind of problems, we analyze the features of limited-angle CT images and propose a multi-scale dilated convolution neural network (MSD-CNN) to correct the artifacts and to restore the image. In this network, the dilated convolution layer and multi-scale pooling layer are combined to form a group and exited in the whole encoder-decoder process. Since the dilated convolutions support an exponential expansion of the receptive field without losing resolution and coverage, the obtained artifact features possess the multi-scale characteristic. Furthermore, to improve the effectiveness and accuracy of the training step, we employ a preprocessing method, which extracts image patches. Numerical experiments verify the out-performance of the proposed method compared with some conventional methods, such as Unet based deep learning,TV- and L0 -based optimization methods.
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- 2020
28. Olmesartan Medoxomil, An Angiotensin II-Receptor Blocker, Ameliorates Renal Injury In db/db Mice
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Ye Zhu, Ze-Liang Li, Weiping Zhu, Tongxia Cui, Huitao Zhang, Ao Ding, Hua Zhang, and Hui Yang
- Subjects
0301 basic medicine ,Pharmacology ,medicine.medical_specialty ,Kidney ,Angiotensin receptor ,business.industry ,Pharmaceutical Science ,Renal function ,medicine.disease ,Streptozotocin ,Angiotensin II ,Diabetic nephropathy ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,030220 oncology & carcinogenesis ,Internal medicine ,Diabetes mellitus ,Drug Discovery ,Medicine ,business ,Olmesartan ,medicine.drug - Abstract
Background Diabetic nephropathy (DN) is a common complication of diabetes mellitus (DM) and also a major cause of end-stage renal disease (ESRD). Olmesartan medoxomil (OM) is an angiotensin II receptor blocker (ARB) and has been shown to exhibit renoprotective effects on a streptozotocin (STZ)-induced diabetic rat model. Yet, whether OM affects DN progression and renal injury in db/db mice, a type 2 diabetic murine model, has not been established. Methods Wild-type (n = 15) and db/db mice (n = 15) were treated with control saline or OM via oral gavage. The physiological and biochemical parameters were evaluated and histological examinations of kidney specimens were performed. Results Compared with saline-treated db/db mice, db/db mice administered with OM showed ameliorated diabetic physiological and biochemical parameters. In addition, OM decreased urinary albumin excretion and plasma creatinine level in db/db mice. Moreover, histologically, OM reduced glomerular hypertrophy and injury, and also ameliorated tubular injury, thus suggesting that OM improves renal function and minimizes renal pathological deterioration in db/db mice. Conclusion Our study reveals a beneficial role of OM in ameliorating DN in db/db mice, which is associated with its renoprotective function.
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- 2019
29. Generation of three lines from multiorgan venous and lymphatic defect syndrome patients
- Author
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Xiaojun Hu, Junjie Mao, Ke Zhang, Huitao Zhang, Dan Li, Bin Zhou, Hong Shan, Bing Li, and Pengfei Pang
- Subjects
DEAD-box RNA Helicases ,QH301-705.5 ,Induced Pluripotent Stem Cells ,Mutation ,Humans ,Cell Differentiation ,Cell Biology ,General Medicine ,Syndrome ,Biology (General) ,Fibroblasts ,Developmental Biology - Abstract
Multiorgan venous and lymphatic defect (MOVLD) syndrome is a unique visceral vascular malformations with complex etiologies. In this study, primary skin fibroblasts were obtained from three MOVLD patients and reprogrammed into iPSCs by Yamanaka’s classical strategy. The MOVLD- iPSCs carrying the DDX24 p.Glu271Lys mutation were confirmed by Sanger sequencing. The pluripotency of MOVLD-iPSCs was verified by the specific molecular markers and gene expression, trilineage differentiation potential. The establishment of the MOVLD-iPSCs will provide a useful model for understanding the mechanisms involved the MOVLD and promoting the development of medical treatment.
- Published
- 2021
30. CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
- Author
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Bin Zhang, Yingying Niu, Xuejing Li, Jisheng Nie, Ye Fu, Hongjie Zhang, Huitao Zhang, Aimin Yang, Jin Yang, and Yanli Liu
- Subjects
Adult ,Male ,China ,medicine.medical_specialty ,Coke oven ,Health, Toxicology and Mutagenesis ,Oxidative dna damage ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:RC963-969 ,Occupational Exposure ,Internal medicine ,Cytochrome P-450 CYP1A1 ,medicine ,polycyclic compounds ,Humans ,Deoxyguanosine ,Cytochrome P4501A1 ,0303 health sciences ,DNA methylation ,business.industry ,Research ,lcsh:Public aspects of medicine ,Smoking ,Public Health, Environmental and Occupational Health ,030311 toxicology ,lcsh:RA1-1270 ,Methylation ,Middle Aged ,Pah exposure ,Polycyclic aromatic hydrocarbons ,Oxidative Stress ,Cross-Sectional Studies ,Endocrinology ,chemistry ,Oxidative DNA damage ,lcsh:Industrial medicine. Industrial hygiene ,Pyrosequencing ,Female ,Co exposure ,business ,DNA Damage - Abstract
Background Multiple factors, including co-exposure between lifestyle and environmental risks, are important in susceptibility to oxidative DNA damage. However, the underlying mechanism is not fully understood. This study was undertaken to evaluate whether Cytochrome P4501A1 (CYP1A1) methylation can mediate the co-exposure effect between smoking and occupational polycyclic aromatic hydrocarbons (PAH) in development of oxidative DNA damage. Methods We explored the associations between smoking and occupational PAH co-exposure effect, CYP1A1 methylation and oxidative DNA damage among 500 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (1-hydroxypyrene, 1-OHP; 2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; and 9-hydroxyphenanthren, 9-PHE) and a marker of oxidative DNA damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. CYP1A1 methylation was measured by pyrosequencing. Finally, mediation analysis was performed to investigate whether CYP1A1 methylation mediated smoking and occupational PAH co-exposure effect on oxidative DNA damage. Results We observed significant associations of smoking and 1-OHP co-exposure with CYP1A1 hypomethylation (OR: 1.87, 95% CI: 1.01–3.47) and high 8-OHdG (OR: 2.13, 95% CI: 1.14–3.97). There was a significant relationship between CYP1A1 hypomethylation and high 8-OHdG (1st vs. 3rd tertile = 1.58, 95% CI: 1.01–2.47, P for trend = 0.046). In addition, mediation analysis suggested CYP1A1 hypomethylation could explain 13.6% of effect of high 8-OHdG related to smoking and 1-OHP co-exposure. Conclusions Our findings suggested that the co-exposure effect of smoking and occupational PAH could increase the risk of oxidative DNA damage by a mechanism partly involving CYP1A1 hypomethylation. Electronic supplementary material The online version of this article (10.1186/s12940-019-0508-0) contains supplementary material, which is available to authorized users.
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- 2019
31. Mediation effect of AhR expression between polycyclic aromatic hydrocarbons exposure and oxidative DNA damage among Chinese occupational workers
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Yanli Liu, Jisheng Nie, Ye Fu, Jinzhu Zhao, Yingying Niu, Hongjie Zhang, Huitao Zhang, Jin Yang, and Aimin Yang
- Subjects
Adult ,Male ,China ,Mediation (statistics) ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Urinary system ,Naphthols ,010501 environmental sciences ,Toxicology ,01 natural sciences ,law.invention ,Oxidative dna damage ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Asian People ,Naphthalenesulfonates ,law ,Occupational Exposure ,Internal medicine ,medicine ,Humans ,Deoxyguanosine ,Polycyclic Aromatic Hydrocarbons ,Coke ,Chromatography, High Pressure Liquid ,Polymerase chain reaction ,Carcinogen ,0105 earth and related environmental sciences ,Aspartic Acid ,Fluorenes ,Pyrenes ,biology ,Chemistry ,General Medicine ,Aryl hydrocarbon receptor ,030210 environmental & occupational health ,Pollution ,Reverse transcriptase ,Oxidative Stress ,Endocrinology ,Receptors, Aryl Hydrocarbon ,8-Hydroxy-2'-Deoxyguanosine ,Carcinogens ,biology.protein ,Environmental Pollutants ,DNA Damage - Abstract
Polycyclic aromatic hydrocarbons (PAH) are well-known to be carcinogenic and the mechanisms that it contributes to oxidative DNA damage and aryl hydrocarbon receptor (AhR)-dependent induction are also well understood. However, little is known about the associations between PAH exposure, AhR expression, and oxidative DNA damage. We investigated their associations of AhR expression and oxidative DNA damage related to PAH exposure among 310 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; 9-hydroxyphenanthren, 9-PHE; and 1-hydroxypyrene, 1-OHP) and a marker of oxidative damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. AhR expression in venous blood was measured by reverse transcription -polymerase chain reaction. The results showed that increasing levels of urinary 1-OHP was positively associated with high 8-OHdG (OR (95% CI) was 4.01 (1.41–11.45) for 4th quartile, compared with 1st quartile, P for trend = 0.013). The similar associations were also found between urinary 1-OHP and high-AhR expressions (4th vs. 1st quartile = 3.50, 95% CI: 1.24–9.87, P for trend = 0.029). A significant association between AhR expression and high 8-OHdG was also found (4th vs. 1st quartile = 2.44, 95% CI: 1.05–5.70, P for trend = 0.027). In addition, mediation analysis showed the AhR expression could explain 35.9% of the association of oxidative DNA damage related to PAH exposure. Our findings implicated that the association between PAH exposure and oxidative DNA damage may be mediated by AhR expression among Chinese occupational workers.
- Published
- 2018
32. A method for material decomposition and quantification with grating based phase CT
- Author
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Peiping Zhu, Qian Wang, Yining Zhu, Huitao Zhang, Shi-Wo Deng, Peng Zhang, and Kai Zhang
- Subjects
Computer science ,Contrast Media ,Optical Analysis ,Computed tomography ,01 natural sciences ,Diagnostic Radiology ,030218 nuclear medicine & medical imaging ,Synchrotron Radiation ,0302 clinical medicine ,Medicine and Health Sciences ,Signal Amplification ,Tomography ,Multidisciplinary ,medicine.diagnostic_test ,Refractive Index ,Phantoms, Imaging ,Radiology and Imaging ,Applied Mathematics ,Simulation and Modeling ,Physics ,Electromagnetic Radiation ,Phase-contrast imaging ,Bone Imaging ,Physical Sciences ,Engineering and Technology ,Medicine ,Algorithm ,Algorithms ,Research Article ,Imaging Techniques ,Iterative method ,Science ,Phase (waves) ,Neuroimaging ,Iterative reconstruction ,Research and Analysis Methods ,010309 optics ,03 medical and health sciences ,Diagnostic Medicine ,0103 physical sciences ,medicine ,Humans ,Computer Simulation ,Chemical Characterization ,Biology and Life Sciences ,Computed Axial Tomography ,X-Ray Radiography ,Noise ,Simultaneous Algebraic Reconstruction Technique ,Signal Processing ,Tomography, X-Ray Computed ,Mathematics ,Neuroscience - Abstract
Material decomposition (MD) is an important application of computer tomography (CT). For phase contrast imaging, conventional MD methods are categorized into two types with respect to different operation sequences, i.e., “before” or “after” image reconstruction. Both categories come down to two-step methods, which have the problem of noise amplification. In this study, we incorporate both phase and absorption (PA) information into MD process, and correspondingly develop a simultaneous algebraic reconstruction technique (SART). The proposed method is referred to as phase & absorption material decomposition-SART (PAMD-SART). By iteratively solving an optimization problem, material composition and substance quantification are reconstructed directly from absorption and differential phase projections. Comparing with two-step MD, the proposed one-step method is superior in noise suppression and accurate decomposition. Numerical simulations and synchrotron radiation based experiments show that PAMD-SART outperforms the classical MD method (image-based and dual-energy CT iterative method), especially for the quantitative accuracy of material equivalent atomic number.
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- 2021
33. Enhancing the X-Ray Differential Phase Contrast Image Quality With Deep Learning Technique
- Author
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Yongshuai Ge, Jinchuan Guo, Huitao Zhang, Peizhen Liu, Jianwei Chen, Yifan Ni, Zhicheng Li, Hairong Zheng, Ting Su, Jiecheng Yang, and Dong Liang
- Subjects
Noise measurement ,business.industry ,Computer science ,Image quality ,X-Rays ,0206 medical engineering ,Biomedical Engineering ,Image processing ,02 engineering and technology ,Grating ,Radiation ,Signal-To-Noise Ratio ,020601 biomedical engineering ,Convolutional neural network ,Signal ,Imaging phantom ,Radiography ,Signal-to-noise ratio ,Deep Learning ,Image noise ,Image Processing, Computer-Assisted ,Computer vision ,Artificial intelligence ,business ,Algorithms - Abstract
Objective: The purpose of this work is to investigate the feasibility of using deep convolutional neural network (CNN) to improve the image quality of a grating-based X-ray differential phase contrast imaging (XPCI) system. Methods: In this work, a novel deep CNN based phase signal extraction and image noise suppression algorithm (named as XP-NET) is developed. The numerical phase phantom, the ex vivo biological specimen and the ACR breast phantom are evaluated via the numerical simulations and experimental studies, separately. Moreover, images are also evaluated under different low radiation levels to verify its dose reduction capability. Results: Compared with the conventional analytical method, the novel XP-NET algorithm is able to reduce the bias of large DPC signals and hence increasing the DPC signal accuracy by more than 15%. Additionally, the XP-NET is able to reduce DPC image noise by about 50% for low dose DPC imaging tasks. Conclusion: This proposed novel end-to-end supervised XP-NET has a great potential to improve the DPC signal accuracy, reduce image noise, and preserve object details. Significance: We demonstrate that the deep CNN technique provides a promising approach to improve the grating-based XPCI performance and its dose efficiency in future biomedical applications.
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- 2020
34. Survey of Anxiety and Depression in Patients with Suspected and Confirmed Cases of COVID-19 During Hospitalization and Isolation
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Zhonghe Li, Yilan Chen, Juan Wang, Xiaohua Gong, Fei Li, Meifang Mai, Dongliang Xia, Huitao Zhang, and Xinming Guo
- Subjects
medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,Isolation (health care) ,business.industry ,Internal medicine ,medicine ,Anxiety ,In patient ,medicine.symptom ,business ,Depression (differential diagnoses) - Abstract
Objective: To observe the anxiety and depression experienced by patients with suspected and confirmed COVID-19 during hospitalization and isolation.Methods: A cross-sectional survey was performed with 66 patients with suspected and confirmed COVID-19 who were admitted to the Fifth Affiliated Hospital of Sun Yat-sen University from February 9, 2020, to February 22, 2020. The data collected including sex, age, education level, and nucleic acid test results. Anxiety, depression, and sleep disorders were evaluated using the Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS) and Pittsburgh Sleep Quality Index Scale (PSQI), respectively. Statistical analysis: SPSS 23.0 software was used for data processing, and multifactor logistic regression analysis was used to identify the independent risk factors. Spearman correlation analysis was used to study the correlations among the observed indicators; P Results: The incidences of anxiety, depression, and sleep disorders in the suspected case group were 18.2%, 18.2%, and 39.4%; the incidences of anxiety, depression, and sleep disorders in the diagnosed group were 42.4%, 57.6%, and 69.7%. The incidence of sleep disorders was higher than the domestic norm, and the difference was statistically significant (P P Conclusion: Patients with suspected and confirmed COVID-19, especially people aged 50 years and over, have significant anxiety, depression and sleep disorders. Anxiety, depression and sleep disorders in patients with confirmed cases are more serious than those in patients with suspected cases. It is necessary to focus on the psychological state of such patients, actively conduct psychological counseling, and reduce their anxiety and depression.
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- 2020
35. Dual phase grating based X-ray differential phase contrast imaging with source grating: theory and validation
- Author
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Jianwei Chen, Dong Liang, Kai Zhang, Peiping Zhu, Hairong Zheng, Yongshuai Ge, Jinchuan Guo, Shi Wei, Shiwo Deng, Huitao Zhang, and Jun Yang
- Subjects
Diffraction ,Physics ,Geometrical optics ,business.industry ,Phase (waves) ,Phase-contrast imaging ,Physics::Optics ,02 engineering and technology ,Grating ,021001 nanoscience & nanotechnology ,Physical optics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,010309 optics ,Interferometry ,Optics ,Thin lens ,0103 physical sciences ,0210 nano-technology ,business - Abstract
In this work, we developed a new theoretical framework using wave optics to explain the working mechanism of the grating based X-ray differential phase contrast imaging (XPCI) interferometer systems consist of more than one phase grating. Under the optical reversibility principle, the wave optics interpretation was simplified into the geometrical optics interpretation, in which the phase grating was treated as a thin lens. Moreover, it was derived that the period of an arrayed source, e.g., the period of a source grating, is always equal to the period of the diffraction fringe formed on the source plane. When a source grating is utilized, the theory indicated that it is better to keep the periods of the two phase gratings different to generate large period diffraction fringes. Experiments were performed to validate these theoretical findings.
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- 2020
36. A Small-Bodied Troodontid (Dinosauria, Theropoda) from the Upper Cretaceous Wulansuhai Formation of Inner Mongolia, China
- Author
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Josef Steigler, Qiyue Zhang, Qingwei Tan, Lin Tan, Huitao Zhang, and Shuo Wang
- Subjects
Sister group ,Philovenator ,Sinovenator ,Anatomy ,Biology ,Body size ,Inner mongolia ,biology.organism_classification ,Theropoda ,Linhevenator ,Cretaceous - Abstract
A new small-bodied troodontid (LH PV39) recovered from the Upper Cretaceous Wulansuhai Formation, Suhongtu, Inner Mongolia, China, is described. The new specimen preserves six postaxial cervical vertebrae, five completely fused sacral and four posterior caudal vertebrae in addition to two manual unguals. The completely fused neurocentral junctions indicate that a skeletally mature individual of the same species of LH PV39 would be smaller than Philovenator and comparable in body size to a skeletal mature individual of Almas. The extremely dorsoventrally compressed sacral centra and neural canal, and the middle three sacral centra that are shorter and wider than the first and the last one distinguishing LH PV39 from other known troodontids. A series of phylogenetic analyses were conducted using modified published matrices. By coding LH PV39 in different strategies, the troodontid affinity of LH PV39 is confirmed and it was recovered as the sister taxon of either Mei and Sinovenator (LH PV39 scored as a separate OTU) or Linhevenator (incorporating LH PV39 into Philovenator) in the best resolved coelurosaurian interrelationships. The referral of LH PV39 to Philovenator does not seriously alter the phylogenetic position of Philovenator nor the interrelationships of troodontids. This new finding confirms that the small and large sized troodontids are coexisted in the Gobi Desert of the Mongolia Plateau until the end of Cretaceous.
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- 2020
37. Understanding working conditions for Entererococcus sp. L1 on methane production: A low temperature condition-based biogas production experiment
- Author
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Wang Xiaou, Yang Xinping, Aihemati Guli, Wang Zhifang, Huitao Zhang, Jingping Dai, Xie Yuqing, Feng Lei, Jiang Li, and Jing Chen
- Subjects
biology ,Strain (chemistry) ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Forestry ,biology.organism_classification ,chemistry.chemical_compound ,Biogas ,Fermentation ,Food science ,Methane production ,Citric acid ,Waste Management and Disposal ,Agronomy and Crop Science ,Cow dung ,Bacteria ,Biogas production - Abstract
To provide an elaborate understanding on the performance of Entererococcus sp. L1 in biogas production at different fermentation periods under controlled low temperature, biogas optimization experiments was undertaken. Specifically, microscopic and culture techniques, physiological and biochemical studies, and measurement of optical densities at 600 wavelengths (OD600) were undertaken to identify and characterize the Entererococcus sp. L1. The aim was to identify the strain and determine its optimum working conditions in biogas production at different fermentation periods under controlled low temperature. The result confirmed the strain as hydrogen-producing bacteria (Enteroccus sp. L1). It was also confirmed that the organic nitrogen source was significantly better than the inorganic nitrogen source in supporting the growth of the bacteria. The optimal pH and temperature for the growth and biogas production performance of the Entererococcus sp. L1 was 7 and 15 °C respectively. The Na2HPO4 concentration of 0.2% was confirmed to be best for the optimum growth of the bacteria, and addition of citric acid resulted to significant inhibitory effect on the growth of the Entererococcus sp. L1. The effect of adding different concentrations (0, 1%, 5%, 10%) of the L1 bacteria inoculum on cow dung at simulated low temperature (15 °C) biogas fermentation showed that 10% treatment had the highest gas production of 3121 ml, which was 89% higher than the control (0 concentration). The daily gas production rate was 0.02 m3/(m−3d−1), which was 90% higher than the control. This showed that 10% treatment provided the best gas production result, with highest gas production occurring at 10% treatment within 31–60 days of fermentation (p
- Published
- 2021
38. Additional file 1: of CYP1A1 methylation mediates the effect of smoking and occupational polycyclic aromatic hydrocarbons co-exposure on oxidative DNA damage among Chinese coke-oven workers
- Author
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Yanli Liu, Xuejing Li, Zhang, Bin, Fu, Ye, Aimin Yang, Hongjie Zhang, Huitao Zhang, Yingying Niu, Jisheng Nie, and Yang, Jin
- Abstract
Table S1. Primers used for pyrosequencing size of the PCR amplicons and position of the primers from the transcription start point. Table S2. Distributions of urine PAH metabolites among 500 occupational workers. Table S3. The correlation coefficients (rs) of urine PAH metabolites among 500 occupational workers. Table S4. The correlation coefficients (rs) of CYP1A1 methylation among 500 occupational workers. Table S5. Contribution rates of smoking on urine PAH metabolites among 500 occupational workers. (DOC 67 kb)
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- 2019
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39. Comparative morphology refines the conventional model of spider reproduction
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He Jiang, Qingqing Wu, Matjaž Kuntner, Huitao Zhang, Lihong Tu, Yongjia Zhan, and Zishang Bai
- Subjects
Male ,0106 biological sciences ,0301 basic medicine ,Uterus ,01 natural sciences ,Internal fertilization ,Animal Cells ,Abdomen ,Medicine and Health Sciences ,Electron Microscopy ,Comparative Anatomy ,Microscopy ,Multidisciplinary ,Reproduction ,Eukaryota ,Spiders ,Anatomy ,medicine.anatomical_structure ,Oviduct ,Medicine ,Female ,Scanning Electron Microscopy ,Cellular Types ,Genital Anatomy ,Research Article ,endocrine system ,Arthropoda ,Science ,Biology ,Research and Analysis Methods ,Models, Biological ,010603 evolutionary biology ,03 medical and health sciences ,Spermatheca ,Arachnida ,Reproductive biology ,medicine ,Animals ,Sex organ ,Spider ,urogenital system ,Reproductive System ,Organisms ,Biology and Life Sciences ,Animal Structures ,Cell Biology ,Invertebrates ,Sperm ,Germ Cells ,030104 developmental biology ,Fertilization ,Zoology ,Developmental Biology - Abstract
Our understanding of spider reproductive biology is hampered by the vast anatomical diversity and difficulties associated with its study. Although authors agree on the two general types of female spider genitalia, haplogyne (plesiomorphic) and entelegyne (apomorphic), our understanding of variation within each group mostly concerns the external genital part, while the internal connections with the reproductive duct are largely unknown. Conventionally and simplistically, the spermathecae of haplogynes have simple two-way ducts, and those of entelegynes have separate copulatory and fertilization ducts for sperm to be transferred in and out of spermathecae, respectively. Sperm is discharged from the spermathecae directly into the uterus externus (a distal extension of the oviduct), which, commonly thought as homologous in both groups, is the purported location of internal fertilization in spiders. However, the structural evolution from haplo- to entelegyny remains unresolved, and thus the precise fertilization site in entelegynes is ambiguous. We aim to clarify this anatomical problem through a widely comparative morphological study of internal female genital system in entelegynes. Our survey of 147 epigyna (121 examined species in 97 genera, 34 families) surprisingly finds no direct connection between the fertilization ducts and the uterus externus, which, based on the homology with basal-most spider lineages, is a dead-end caecum in entelegynes. Instead, fertilization ducts usually connect with a secondary uterus externus, a novel feature taking over the functional role of the plesiomorphic uterus externus. We hypothesize that the transition from haplo- to entelegyny entailed not only the emergence of the two separate duct systems (copulatory, fertilization), but also involved substantial morphological changes in the distal part of the oviduct. Thus, the common oviduct may have shifted its distal connection from the uterus externus to the secondary uterus externus, perhaps facilitating discharge of larger eggs. Our findings suggest that the conventional model of entelegyne reproduction needs redefinition.
- Published
- 2019
40. 3D Hessian deconvolution of thick light-sheet z-stacks for high-contrast and high-SNR volumetric imaging
- Author
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Ruyi Zheng, Ke Du, Liangyi Chen, Hongrun Yang, Zhe Zhang, Huitao Zhang, Dongzhou Gou, Fan Feng, Zhang Guangyi, Louis Tao, and Heng Mao
- Subjects
Hessian matrix ,Physics ,Deblurring ,Pixel ,Optical sectioning ,business.industry ,Image processing ,02 engineering and technology ,Iterative reconstruction ,021001 nanoscience & nanotechnology ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,010309 optics ,symbols.namesake ,Optics ,0103 physical sciences ,symbols ,Depth of field ,Deconvolution ,0210 nano-technology ,business - Abstract
Due to its ability of optical sectioning and low phototoxicity, z -stacking light-sheet microscopy has been the tool of choice for in vivo imaging of the zebrafish brain. To image the zebrafish brain with a large field of view, the thickness of the Gaussian beam inevitably becomes several times greater than the system depth of field (DOF), where the fluorescence distributions outside the DOF will also be collected, blurring the image. In this paper, we propose a 3D deblurring method, aiming to redistribute the measured intensity of each pixel in a light-sheet image to in situ voxels by 3D deconvolution. By introducing a Hessian regularization term to maintain the continuity of the neuron distribution and using a modified stripe-removal algorithm, the reconstructed z -stack images exhibit high contrast and a high signal-to-noise ratio. These performance characteristics can facilitate subsequent processing, such as 3D neuron registration, segmentation, and recognition.
- Published
- 2020
41. A large enantiornithine bird from the Lower Cretaceous of China and its implication for lung ventilation
- Author
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Defeng Chen, Zihui Zhang, Lianhai Hou, and Huitao Zhang
- Subjects
Rib cage ,biology ,Enantiornithes ,Jiufotang Formation ,Anatomy ,musculoskeletal system ,biology.organism_classification ,Cretaceous ,Time of death ,medicine.anatomical_structure ,Thoracic vertebrae ,medicine ,Mesozoic ,Lung ventilation ,Ecology, Evolution, Behavior and Systematics - Abstract
The Enantiornithes were the most taxonomically diverse bird group in the Mesozoic. Most of the known taxa are from Lower Cretaceous deposits of the Jehol Group in north-eastern China. A new specimen from the Jiufotang Formation in Jianchang, Liaoning Province, is described here; being a subadult individual at the time of death it had reached a relatively large size. The presence of uncinate processes, bicapitate and forked vertebral ribs, sternal ribs that were all of similar length, as well as the location of parapophyses and diapophyses on the thoracic vertebrae, may imply a rigid and volume-constant lung, and less efficient lung ventilation in enantiornithines. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 113, 820–827. ADDITIONAL KEYWORDS: Jianchang – Jiufotang Formation – Liaoning province – respiratory apparatus – ribs – thoracic vertebrae.
- Published
- 2014
42. Quantitative coherence analysis with an X-ray Talbot–Lau interferometer
- Author
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Peiping Zhu, Wanxia Huang, Ziyu Wu, Qingxi Yuan, Youli Hong, Xiaosong Liu, Kai Zhang, Kun Gao, Xin Ge, Zhili Wang, and Huitao Zhang
- Subjects
Physics ,Interferometric visibility ,business.industry ,Astrophysics::High Energy Astrophysical Phenomena ,Physics::Optics ,Degree of coherence ,Grating ,Biochemistry ,Analytical Chemistry ,Generator (circuit theory) ,Interferometry ,Optics ,Point (geometry) ,Physics::Atomic Physics ,Absorption (electromagnetic radiation) ,business ,Visibility - Abstract
Differential phase-contrast (DPC) X-ray imaging has been performed in the Talbot-Lau configuration, in which the X-ray source was a combination of an absorption grating and a laboratory X-ray generator. We report here quantitative analysis of partial coherence effects on the X-ray Talbot-Lau interferometer. Based on the visibility of the self-image, the well-known geometry condition is reproduced. It is shown that effects of partial coherence are determined by the opening ratio of the source grating, and that the effects are independent of the Talbot order and the type of the phase grating, a condition quite different from those in a Talbot interferometer. A possible explanation is discussed from the point of view of the effective spatial coherence length. Taking into account the available X-ray flux and experimental fluctuations, we present the optimum opening ratio. Furthermore, we mention that our results can also be successfully used to discuss the properties of a multiline X-ray source.
- Published
- 2010
43. BPF-based reconstruction algorithm for multiple rotation–translation scan mode
- Author
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Peng Zhang, Ming Chen, and Huitao Zhang
- Subjects
business.industry ,Process (computing) ,Reconstruction algorithm ,Industrial computed tomography ,Translation (geometry) ,Feature (computer vision) ,General Materials Science ,Computer vision ,Artificial intelligence ,business ,General ,Rotation (mathematics) ,Image resolution ,Interpolation ,Mathematics - Abstract
In industrial CT, it is often required to inspect large objects using short line-detectors. To acquire the complete CT data for the scanning slice of large objects using short line-detectors, some multi-scan modes have been developed. But the existing methods reconstructing an image from the data scanned by multi-scan modes have to rebin the data into fan-beam or parallel-beam form via data interpolation. The data rebinning process not only increases great computational cost, but also degrades image resolution. In this paper, we propose a backprojection-filtration (BPF)-based reconstruction algorithm for rotation–translation (RT) multi-scan mode. An important feature of the proposed algorithm is that data rebinning process is not introduced. The simulation results have verified the validity of the proposed algorithm.
- Published
- 2008
- Full Text
- View/download PDF
44. Bovine parathyroid hormone enhances osteoclast bone resorption by modulating V-ATPase through PTH1R
- Author
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Zhuo Li, Xinling Liang, Bin Zhang, Li Zhang, Jianchao Ma, Huitao Zhang, Zhonghe Li, Weiping Zhu, Wei Shi, Shuangxin Liu, and Sijia Li
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Vacuolar Proton-Translocating ATPases ,Parathyroid hormone ,Osteoclasts ,Biology ,vacuolar-type H+ adenosine triphosphatase ,Bone resorption ,Bone remodeling ,03 medical and health sciences ,Mice ,Western blot ,Osteoclast ,Bone Marrow ,Internal medicine ,Genetics ,medicine ,V-ATPase ,Animals ,Humans ,Bone Resorption ,Receptor ,Receptor, Parathyroid Hormone, Type 1 ,medicine.diagnostic_test ,General Medicine ,Articles ,Resorption ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,Parathyroid Hormone ,proton pump ,osteoclast ,Cattle ,Bone Remodeling ,Macrolides - Abstract
The vacuolar-type H+ adenosine triphosphatase (V-ATPase) plays an important role in cellular acidification and bone resorption by osteoclasts. However, the direct effect of bovine parathyroid hormone (bPTH) on V-ATPase has not yet been elucidated. The aim of the present study was to assess the effects of bPTH on V-ATPase and osteoclasts. Osteoclasts from bone marrow (BM)-derived monocytes of C57BL/6 mice were cultured with or without bPTH. The mRNA and protein expression levels of the V-ATPase a3-subunit and d2-subunit (by RT-qPCR and western blot analysis), V-ATPase activity (using the V type ATPase Activity Assay kit) and the bone resorption function of osteoclasts (by bone resorption assay) were examined following treatment with various concentrations of bPTH (0.1, 1.0, 10 and 100 ng/ml) alone or with bPTH and its inhibitor, bafilomycin A1. Furthermore, the expression of parathyroid hormone (PTH) receptors in osteoclasts was also detected. The results revealed that the mRNA and protein expression levels of V-ATPase a3-subunit and d2-subunit increased in a dose‑dependent manner, paralleling the level of bPTH present. In addition, an increase in the concentration of bPTH was accompanied by the increased resorption capability of osteoclasts, whereas bone resorption was inhibited in the presence of bafilomycin A1. In addition, we confirmed the existence of parathyroid hormone 1 receptor (PTH1R) in osteoclasts using three different methods (RT-qPCR, western blot analysis and immunofluorescence staining). We found that bPTH enhanced the bone resorption capability of osteoclasts by modulating the expression of V-ATPase subunits, intracellular acidification and V-ATPase activity. Thus, we propose that PTH has a direct effect on osteoblasts and osteoclasts, and that this effect is mediated through PTH1R, thus contributing to bone remodeling.
- Published
- 2015
45. Expression and clinical evidence of miR-494 and PTEN in non-small cell lung cancer
- Author
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Nan Chen, Juan Wang, Yi-feng Liao, Huitao Zhang, Hongyu Zhang, Hongtao Chen, and Tianze Liu
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,In situ hybridization ,Kaplan-Meier Estimate ,Biology ,Malignancy ,Disease-Free Survival ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,medicine ,Carcinoma ,Biomarkers, Tumor ,Tensin ,PTEN ,Humans ,Clinical significance ,Lung cancer ,In Situ Hybridization ,Aged ,Neoplasm Staging ,Aged, 80 and over ,PTEN Phosphohydrolase ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Lymphatic Metastasis ,biology.protein ,Immunohistochemistry ,Female - Abstract
The aim of this study was to explore the expression and clinical significance of miR-494 and PTEN (phosphatase and tensin homologue deleted on chromosome ten) in non-small cell lung cancer (NSCLC). Immunohistochemistry for PTEN and in situ hybridization (ISH) for miR-494 were performed in 92 NSCLC tissues and 10 normal lung tissues to detect their expression, and correlation between their expression with clinical characteristics and prognosis was analyzed. The expression of miR-494 was significantly higher in NSCLC than in normal lung tissues (P = 0.004). The positive expression of PTEN protein in the lung carcinoma tissues was significantly lower than that in the normal lung tissues (P = 0.013), while the level of miR-494 expression was negatively correlated with PTEN expression (r = −0.577, P
- Published
- 2014
46. Bovine parathyroid hormone enhances osteoclast bone resorption by modulating V-ATPase through PTH1R.
- Author
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SHUANGXIN LIU, WEIPING ZHU, SIJIA LI, JIANCHAO MA, HUITAO ZHANG, ZHONGHE LI, LI ZHANG, BIN ZHANG, ZHUO LI, XINLING LIANG, and WEI SHI
- Published
- 2016
- Full Text
- View/download PDF
47. Quantitative coherence analysis with an X-ray Talbot–Lau interferometer.
- Author
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Zhili Wang, Peiping Zhu, Wanxia Huang, Qingxi Yuan, Xiaosong Liu, Kai Zhang, Youli Hong, Huitao Zhang, Xin Ge, Kun Gao, and Ziyu Wu
- Subjects
QUANTITATIVE chemical analysis ,INTERFEROMETERS ,OPTICAL instruments ,COHERENCE (Optics) ,X-rays - Abstract
Differential phase-contrast (DPC) X-ray imaging has been performed in the Talbot–Lau configuration, in which the X-ray source was a combination of an absorption grating and a laboratory X-ray generator. We report here quantitative analysis of partial coherence effects on the X-ray Talbot–Lau interferometer. Based on the visibility of the self-image, the well-known geometry condition is reproduced. It is shown that effects of partial coherence are determined by the opening ratio of the source grating, and that the effects are independent of the Talbot order and the type of the phase grating, a condition quite different from those in a Talbot interferometer. A possible explanation is discussed from the point of view of the effective spatial coherence length. Taking into account the available X-ray flux and experimental fluctuations, we present the optimum opening ratio. Furthermore, we mention that our results can also be successfully used to discuss the properties of a multiline X-ray source. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
48. Analysis of polychromaticity effects in X-ray Talbot interferometer.
- Author
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Zhili Wang, Peiping Zhu, Wanxia Huang, Qingxi Yuan, Xiaosong Liu, Kai Zhang, Youli Hong, Huitao Zhang, Xin Ge, Kun Gao, and Ziyu Wu
- Subjects
INTERFEROMETERS ,X-rays ,OPTICAL instruments ,POLARIZATION interferometers ,OPTICAL interferometers - Abstract
The influence of polychromaticity of the X-ray source on the performance of an X-ray Talbot interferometer applied for phase-contrast imaging is analyzed through numerical simulations based on the Fresnel diffraction theory. The presented simulation results show that the visibility of the self-image is fairly insensitive to the source polychromaticity and explain why the interferometer could be well combined with polychromatic X-ray sources in recent experiments. Furthermore, the self-image with a high visibility can be obtained under polychromatic illumination even at a high-order fractional Talbot distance. This fact implies that the acquired image quality for phase measurements can be improved, since the primary signal for phase measurement is proportional to the inter-grating distance. Finally, we mention that the results are also valid for Talbot–Lau interferometer and scanning double-grating configuration. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
49. Think of Several Issues on Precision Guided Weapons Intelligentizing
- Author
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Fan Huitao, Zhang Xinchao
- Subjects
precision guided weapons ,artificial intelligence ,transfer learning ,the combination of intuition and rationality ,Motor vehicles. Aeronautics. Astronautics ,TL1-4050 - Abstract
This article introduces the role of artificial intelligence technology in advancing the intlelligence of precision Guided weapons, outlines the current status of intelligence in it’s detection and recognizing, guidance and control. It discusses the problems faced during the intelligentization process, such as the construction of large samples, transferring simulation performance to real-world capabilities, issues with interptetability and reliability, and the assessment of intelligent weapon performance. Targeted solutions are pesented, including hardware-in-the-loop simulation training, transfer learning, combining human intuition with rationality, and reconstructiong evaluation methods. Three impacts of intelligent precision guided weapons on equipment development and operational use were proposed: increasing importance of simulation technology, significant changed in training patterns, and existing conmbat modes will be overturned.
- Published
- 2024
- Full Text
- View/download PDF
50. Intelligent Classification and Characteristics of Missile Weaponry
- Author
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Fan Huitao, Zhang Tonghe, Zhang Liang
- Subjects
intelligence ,missile ,intelligent classification ,intelligent feature ,weaponry ,Motor vehicles. Aeronautics. Astronautics ,TL1-4050 - Abstract
This paper discusses the intelligent classification and characteristics of missile weaponry, and defines the concept and connotation of intelligent missile. Based on the principle of algorithm design and the development characteristics of missile weaponry, the intelligent level of missile is divided into four levels, that are the initial level, the medium level, the high level and the super level, and the internal meaning of each level is expounded. This paper focuses on the intelligent characteristics of missile weaponry, and describes the capability performance of four levels of intelligent missiles, also discusses the key development technologies of intelligent missile, and introduces the research focus of various key technologies at the current stage based on the current technical status.
- Published
- 2022
- Full Text
- View/download PDF
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