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Mediation effect of AhR expression between polycyclic aromatic hydrocarbons exposure and oxidative DNA damage among Chinese occupational workers
- Source :
- Environmental Pollution. 243:972-977
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Polycyclic aromatic hydrocarbons (PAH) are well-known to be carcinogenic and the mechanisms that it contributes to oxidative DNA damage and aryl hydrocarbon receptor (AhR)-dependent induction are also well understood. However, little is known about the associations between PAH exposure, AhR expression, and oxidative DNA damage. We investigated their associations of AhR expression and oxidative DNA damage related to PAH exposure among 310 workers from a coke-oven plant in China. Urine biomarkers of PAH exposure (2-hydroxynaphthalene, 2-NAP; 2-hydroxyfluorene, 2-FLU; 9-hydroxyphenanthren, 9-PHE; and 1-hydroxypyrene, 1-OHP) and a marker of oxidative damage (8-hydroxy- 2′- deoxyguanosine, 8-OHdG) were measured by high performance liquid chromatography. AhR expression in venous blood was measured by reverse transcription -polymerase chain reaction. The results showed that increasing levels of urinary 1-OHP was positively associated with high 8-OHdG (OR (95% CI) was 4.01 (1.41–11.45) for 4th quartile, compared with 1st quartile, P for trend = 0.013). The similar associations were also found between urinary 1-OHP and high-AhR expressions (4th vs. 1st quartile = 3.50, 95% CI: 1.24–9.87, P for trend = 0.029). A significant association between AhR expression and high 8-OHdG was also found (4th vs. 1st quartile = 2.44, 95% CI: 1.05–5.70, P for trend = 0.027). In addition, mediation analysis showed the AhR expression could explain 35.9% of the association of oxidative DNA damage related to PAH exposure. Our findings implicated that the association between PAH exposure and oxidative DNA damage may be mediated by AhR expression among Chinese occupational workers.
- Subjects :
- Adult
Male
China
Mediation (statistics)
medicine.medical_specialty
Health, Toxicology and Mutagenesis
Urinary system
Naphthols
010501 environmental sciences
Toxicology
01 natural sciences
law.invention
Oxidative dna damage
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Asian People
Naphthalenesulfonates
law
Occupational Exposure
Internal medicine
medicine
Humans
Deoxyguanosine
Polycyclic Aromatic Hydrocarbons
Coke
Chromatography, High Pressure Liquid
Polymerase chain reaction
Carcinogen
0105 earth and related environmental sciences
Aspartic Acid
Fluorenes
Pyrenes
biology
Chemistry
General Medicine
Aryl hydrocarbon receptor
030210 environmental & occupational health
Pollution
Reverse transcriptase
Oxidative Stress
Endocrinology
Receptors, Aryl Hydrocarbon
8-Hydroxy-2'-Deoxyguanosine
Carcinogens
biology.protein
Environmental Pollutants
DNA Damage
Subjects
Details
- ISSN :
- 02697491
- Volume :
- 243
- Database :
- OpenAIRE
- Journal :
- Environmental Pollution
- Accession number :
- edsair.doi.dedup.....3f24cfeced6bb57429997f9010929ebb