43 results on '"Yan, Hua-Zheng"'
Search Results
2. Clinical characteristic of familial exudative vitreous retinopathy occurring in premature infants
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Jin-Rong Li, Qi Zhang, Yan-Hua Zheng, Xiao-Chun Mao, and Jing-Jing Li
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familial exudative vitreoretinopathy ,retinopathy of prematurity ,avascular zone ,retinal vascular zone ,fluorescein angiograms ,Ophthalmology ,RE1-994 - Abstract
AIM: To describe the clinical characteristics of 6 premature infants diagnosed as familial exudative vitreoretinopathy(FEVR).METHODS: From August 2018 to January 2019, the researchers collected six premature cases of FEVR from Xinhua Hospital Affiliated To Shanghai Jiao Tong University School of Medicine. All 6 infants born prematurely had examinations of fundus photography and fluorescein angiograms under anesthesia. Medical history and angiographic features were analyzed retrospectively.RESULTS: Six infants born prematurely were initially misdiagnosed as retinopathy of prematurity ROP. All underwent injection anti-vascular endothelial growth factor(anti-VEGF)drug into vitreous body cavity subsequently, two of whom were treated with injection anti-VEGF drug into vitreous body cavity twice. Six infants born prematurely had follow-up examinations of fundus photography and fluorescein angiograms with the machine of Retcam digital imaging system under anesthesia, they were eventually diagnosed as FEVR. Then 2 cases were treated with laser photocoagulation, 1 case was treated with injection anti-VEGF drug into vitreous body cavity combined laser photocoagulation, 1 case was treated with injection anti-VEGF drug into vitreous body cavity, 2 cases maintain the follow-up visit. CONCLUSION: Clinically, premature infants FEVR, tend to be misdiagnosed as ROP initially. If the demarcation line separating the avascular from the vascular retinal regions presents persistent or the condition turns to be worse, more examinations will be required to confirm the diagnosis such as fluorescein angiograms under anesthesia. FEVR is a lifelong disease, its symptoms, if present, typically take a progressive course during childhood and adolescence. Early diagnosis of FEVR is crucial due to its progressive nature and the genetic/familial underpinnings of the condition. The correct identification of those FEVR patients can help them receive timely treatment and genetic counseling for those of child-bearing age.
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- 2022
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3. MSC Senescence-Related Genes Are Associated with Myeloma Prognosis and Lipid Metabolism-Mediated Resistance to Proteasome Inhibitors
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Yang-Jia Cao, Yan-Hua Zheng, Qing Li, Jin Zheng, Li-Tian Ma, Can-Jun Zhao, and Tian Li
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Article Subject ,Oncology - Abstract
Background. Complex carcinogenic mechanisms and the existence of tumour heterogeneity in multiple myeloma (MM) prevent the most commonly used staging system from effectively interpreting the prognosis of patients. Since the microenvironment plays an important role in driving tumour development and MM occurs most often in middle-aged and elderly patients, we hypothesize that ageing of bone marrow mesenchymal stem cells (BM-MSCs) may be associated with the progression of MM. Methods. In this study, we collected the transcriptome data on MM from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. Differentially expressed genes in both senescent MSCs and MM tumour cells were considered relevant damaged genes. GO and KEGG analyses were applied for functional evaluation. A PPI network was constructed to identify hub genes. Subsequently, we studied the damaged genes that affected the prognosis of MM. Least absolute shrinkage and selection operator (lasso) regression was used to identify the most important features, and a risk model was created. The reliability of the risk model was evaluated with the other 3 GEO validation cohorts. In addition, ROC analysis was used to evaluate the novel risk model. An analysis of immune checkpoint-related genes, tumour immune dysfunction and exclusion (TIDE), and immunophenotypic scoring (IPS) were performed to assess the immune status of risk groups. pRRophetic was utilized to predict the sensitivity to administration of chemotherapeutic agents. Results. We identified that MAPK, PI3K, and p53 signalling pathways were activated in both senescent MSCs and tumour cells, and we also located hub genes. In addition, we constructed a 14-gene prognostic risk model, which was analysed with the ROC and validated in different datasets. Further analysis revealed significant differences in predicted risk values across the International Staging System (ISS) stage, sex, and 1q21 copy number. A high-risk group with higher immunogenicity was predicted to have low proteasome inhibitor sensitivity and respond poorly to immunotherapy. Lipid metabolism pathways were found to be significantly different between high-risk and low-risk groups. A nomogram was created by combining clinical data, and the optimization model was further improved. Finally, real-time qPCR was used to validate two bortezomib-resistant myeloma cell lines, and the test confirmed that 10 genes were detected to be expressed in resistant cell lines with the same trend as in the high-risk cohort compared to nonresistant cells. Conclusion. Fourteen genes related to ageing in BM-MSCs were associated with the prognosis of MM, and by combining this genotypic information with clinical factors, a promising clinical prognostic model was established.
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- 2022
4. Primary lymphoma of bone: a population-based study of 2558 patients
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Chen-Xin Liu, Tian-Qi Xu, Li Xu, Pan-Pan Wang, Chun Cao, Guang-Xun Gao, and Yan-Hua Zheng
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Background: Primary lymphoma of bone (PLB) is an extremely rare malignancy arising in the skeletal system. There is no consensus over the best definition of PLB. Most of the published articles are single-institutional retrospective studies with a limited sample size. The rarity of PLB and discrepancies on diagnostic criteria has resulted in a vague understanding of PLB. Methods We retrospectively analyzed the clinical characteristics and prognostic factors of 2558 PLB patients who were registered in the Surveillance, Epidemiology, and End Results (SEER) database from 1973 to 2016. Survival rates were calculated using the Kaplan–Meier method. The effects of various factors on survival outcomes were analyzed by using the log-rank test. Univariate and multivariate analyses were conducted by using the Cox proportional hazards model to determine independent prognostic factors. Results: The median follow-up time of all eligible patients was 58 months. There seemed no sex preponderance in PLB incidence. The most involved sites are axial skeletons. The most common histological subtype was diffuse large B-cell lymphoma. The 3-, 5-, 10-, and 20-year overall survival (OS) rates were 70.70%, 65.70%, 54.40% and 39.50%, respectively. PLB patients whose primary tumor sites were appendicular and craniofacial skeletons had a significant survival advantage [hazard ratio (HR) = 0.694, 95% confidence interval (CI) 0.552–0.872; HR = 0.729, 95% CI 0.597–0.889, respectively] over those with axial skeletons as primary tumor sites. Patients with Hodgkin lymphoma, non-Hodgkin lymphoma (NHL)–mature B-cell lymphoma, and NHL-precursor-cell lymphoblastic lymphoma also had a significant OS advantage (HR = 0.392, 95% CI 0.200–0.771; HR = 0.826, 95% CI 0.700–0.973; and HR = 0.453, 95% CI 0.223–0.923, respectively). Patients with Ann Arbor stage III–IV at diagnosis were at higher risk of death than those with stage I–II (HR = 1.348, 95% CI 1.107–1.641). Chemotherapy was an independent favorable prognostic factor (HR = 0.734, 95% CI 0.605–0.890). Conclusions: Primary anatomic site, histology type, higher Ann Arbor stage and chemotherapy were independent prognostic factors. Chemotherapy played a pivotal role in PLB treatment.
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- 2020
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5. BRD4 inhibitor nitroxoline enhances the sensitivity of multiple myeloma cells to bortezomib and by promoting mitochondrial pathway-mediated cell apoptosis
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Guang Li, Yan-Hua Zheng, Li Xu, Juan Feng, Hai-Long Tang, Cheng Luo, Yan-Ping Song, and Xie-Qun Chen
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Background: Multiple myeloma (MM) is the second most common hematological neoplasm. Wide administration of bortezomib significantly improves the survival of MM patients compared with conventional chemotherapy. Bromodomain-containing protein 4 (BRD4) inhibitors also have been demonstrated to retard cell proliferation and induce cellular apoptosis in various cancers. However, it is unclear whether the BRD4 inhibitor nitroxoline plus bortezomib has a synergistic anti-tumor effect on MM. Methods: Cell viability was determined via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle and cell apoptosis were assessed via flow cytometry. Protein expression levels were determined via western blotting. The expression of apoptosis-related proteins in xenograft tissue were detected by means of immunohistochemistry. Results: Treatment with nitroxoline or bortezomib suppressed cell proliferation, and caused G0/G1 phase arrest and apoptosis in H929 and RPMI8226 cells. Furthermore, nitroxoline intensified the retardation of cell proliferation, as well as further enhanced the G0/G1 phase arrest and apoptosis induced by bortezomib in H929 and RPMI8226 cells. The western blot analysis revealed that nitroxoline or bortezomib treatment markedly diminished the levels of Bcl-2 and cyclin D1, and increased the levels of p21, Bax, cleaved PARP and cleaved caspase-3. Combination of these two agents was observed to result in further marked changes on these levels compared with nitroxoline or bortezomib treatment alone. What is more, in the xenograft tumor model, combinative treatment markedly inhibited tumor growth compared with the single drug treatment. Conclusion: Combination of bortezomib with nitroxoline has a synergistic anti-tumor activity in MM cells and may be a novel treatment method for MM.
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- 2020
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6. Pulmonary Lymphomatoid Granulomatosis With Hemophagocytic Lymphohistiocytosis as the Initial Manifestation
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Li Xu, Xuan Zhang, Ying-Juan Lu, Yan-Hua Zheng, and Guang-Xun Gao
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lymphomatiod granulomatosis ,hemophagocytic lymphohistiocytosis ,pathology ,rare lymphoma ,Epstein-Barr virus ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Lymphomatoid granulomatosis (LYG) is an extremely rare angio-centric and angio-destructive B-cell lymphoproliferative disease. Driven by Epstein-Barr virus (EBV), LYG predominantly involves the bilateral lungs. Commonly presenting as multiple nodules in the lung, pulmonary LYG can masquerade as various infectious diseases, vasculitis, lung cancer, or other metastatic neoplasm. It is difficult to be diagnosed and is always neglected by clinicians. No standardized therapeutic regimens for LYG has been established yet now. Hemophagocytic lymphohistiocytosis (HLH), a life-threatening condition caused by abnormal activation of macrophages and T-cells, is characterized by fever, hepatosplenomegaly, pancytopenia, hypercytokinemia, and the presence of hemophagocytosis within the bone marrow, liver, spleen, or other lymphatic tissue. We herein report a 55-year-old man with recurrent fever, severe jaundice, and multiple high-density opacities and nodules in both lungs, who was finally diagnosed with pulmonary LYG (Grade 3) manifested with secondary HLH. Administration of HLH-1994 protocol led to the rapid control of the symptoms caused by HLH. Rituximab-based combination therapy was useful yet LYG (Grade 3) progressed rapidly. This case demonstrates that tissue biopsy is essential for early pathological diagnosis and effective treatment of LYG.
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- 2020
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7. Distribution and survival outcomes of primary head and neck hematolymphoid neoplasms in older people: a population-based study
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Yan-Hua Zheng, Biao Tian, Wei-Wei Qin, Qing-Wen Zhu, Juan Feng, Wu-Yue Hu, Ren-An Chen, and Li Liu
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General Medicine ,General Biochemistry, Genetics and Molecular Biology - Published
- 2023
8. [Clinical Characteristics and Prognosis of Systemic Anaplastic Large Cell Lymphoma]
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Juan, Feng, Hai-Long, Tang, Rui-Feng, Yuan, Li, Xu, Yan-Hua, Zheng, Rong, Liang, Qing-Xian, Bai, Tao, Zhang, Lan, Yang, Hong-Tao, Gu, and Guang-Xun, Gao
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Adult ,Male ,Adolescent ,Receptor Protein-Tyrosine Kinases ,Middle Aged ,Prognosis ,Disease-Free Survival ,Young Adult ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Lymphoma, Large-Cell, Anaplastic ,Female ,Child ,Aged ,Retrospective Studies - Abstract
To evaluate the clinical characteristics, treatment and prognosis of systemic anaplastic large cell lymphoma(sALCL).The clinical data of 90 cases with sALCL treated in the Department of Hematology of the Affiliated Xijing Hospital of Air Force Medical University from November 2018 to October 2021 were retrospectively analyzed. The clinical features, treatment and prognosis were summarized and the prognostic factors were investigated.There were 58 males and 32 females, with a median age of 32 (12-73) years old. 69 (76.7%) patients had Ann Arbor stage Ⅲ-Ⅳ disease and half of the patients had extranodal infiltration. The median age was 27(12-72) years of the 60 ALKsALCL mainly occurs in males and most patients were in advanced stage. Half of the patients had extranodal involvement. The CR rate after first-line chemotherapy was 568%, and IPI score≥3 was a significant prognostic factor for CR. No achievement of CR after first-line therapy is poorly prognostic for PFS and OS.系统性间变大细胞淋巴瘤患者的临床特点及预后分析.评价系统性间变大细胞淋巴瘤(sALCL)患者的临床特征、治疗及预后.回顾性分析2018年11月至2021年10月空军军医大学西京医院血液内科收治的90例sALCL患者的临床资料,总结其临床特点、治疗及预后,并探讨预后影响因素.90例sALCL患者中男性58例,女性32例,中位年龄32(12-73)岁。69例(76.7%)患者发病时Ann Arbor 分期为Ⅲ-Ⅳ期,半数患者有结外病变。ALKsALCL以男性居多,诊断时多处于进展期,50%患者合并结外病变。一线化疗后CR率为56.8%,IPI≥3分是一线化疗后CR的不良影响因素。一线化疗后未达CR是PFS及OS 的不良预后因素.
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- 2022
9. Clinical Characteristics and Prognostic Analysis of Multiple Myeloma with Extramedullary Disease: A SEER-Based Study
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Zhen-Zhen Li, Yan-Ping Song, Yao Lv, Yan-Hua Zheng, and Guang Li
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End results ,medicine.medical_specialty ,Article Subject ,business.industry ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Age at diagnosis ,Lower risk ,medicine.disease ,Gastroenterology ,Disease course ,03 medical and health sciences ,0302 clinical medicine ,Oncology ,Extramedullary disease ,030220 oncology & carcinogenesis ,Internal medicine ,Epidemiology ,Medicine ,business ,RC254-282 ,Survival analysis ,Multiple myeloma ,Research Article ,030215 immunology - Abstract
Background. Extramedullary disease (EMD), an infrequent manifestation of multiple myeloma (MM), can present at diagnosis or develop during the disease course. EMD can be clinically divided into bone-related EMD (EMD-B) and soft tissue-related EMD (EMD-S). The purpose of our study is to investigate the clinical characteristics, survival outcomes, and prognostic factors of MM patients with EMD. Methods. A total of 155 MM patients with EMD were ultimately enrolled in our study by retrieving the Surveillance, Epidemiology, and End Results (SEER) database. The Kaplan–Meier survival curves and log-rank test for overall survival (OS) and myeloma-specific survival (MSS) were conducted to compare each potential variable. Variables with a p value 1 representing adverse prognostic factors. Results. The median age at diagnosis was 63 years old. EMD-B occurred in 99 patients (63.90%), while EMD-S occurred in 56 cases (36.10%). Patients with EMD-S had a significant survival disadvantage in MSS (HR = 1.844, 95% CI 1.117–3.042, p = 0.017) and OS (HR = 1.853, 95% CI 1.166–2.942, p = 0.009) compared to those with EMD-B. Patients with EMD interval ≤24 months were at higher risk of death than those with EMD at diagnosis in MSS (HR = 1.885, 95% CI 1.175–3.346, p = 0.042) and in OS (HR = 1.33, 95% CI 1.119–2.529, p = 0.036). Patients with EMD interval >24 months were at a lower risk of death as opposed to those with EMD at diagnosis. Conclusion. Age at MM diagnosis, site of EMD, and time interval from diagnosis to EMD occurrence were independent prognostic factors in MM patients with EMD. EMD-B bore a better prognosis than EMD-S.
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- 2021
10. Clinicopathologic characteristics, therapeutic modalities and survival outcomes of plasmablastic lymphoma: A real-world study
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Yan-Hua Zheng, Kun Xie, Hong-Yuan Shen, Zhuo Wan, Shan Gao, Wen-Rui Sun, Guang-Xun Gao, Li Liu, and Juan Feng
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General Medicine - Abstract
IntroductionPlasmablastic lymphoma(PBL),an extremely rare subtype of B-cell non-Hodgkin lymphoma, is characterized by aggressiveness, rapid progression and bleak prognosis. Neither standardized regimen nor consensus for PBL treatment has been established.Material and methodsWe retrospectively analyzed the clinicopathologic characteristics, therapeutic modalities and survival outcomes of 418 patients registered in the Surveillance, Epidemiology, and End Results (SEER) database from 2008 to 2016 and 21 (19 treated) patients in our institution. Kaplan-Meier survival curves and log-rank test for overall survival and disease-specific survival were performed to compare each variable. Variables with statistical significance in the univariate Cox regression were incorporated into the multivariate model to determine the independent prognostic factors.ResultsIn patient cohort from SEER, PBL has a striking male predilection. The median OS for all PBL patients was 17 months. The 1-year,3-year and 5-year OS rate were 54.4%, 40.4% and 37.2% respectively.Chemotherapy alone or chemotherapy combined with radiotherapy could significantly reduce the risk of death and extend the patients’ survival, yielding HR of 0.209(95%CI 0.152-0.288) and 0.187(95%CI 0.089-0.394), respectively. Radiation alone seemed useless. All patients from our institution were HIV-negative. The main therapeutic regimens were CHOP or CHOPE, DA-EPOCH, DHAP and ESHAP. Complete response was achieved in only 3 patients, while partial response in 10 patients. The median OS was 7 months. Fourteen patients later died of the disease progression.ConclusionsPrevious malignancy history, Ann Arbor stage and therapeutic modality were independent prognostic factors. Bortezomib combined with DA-EPOCH may serve as an effective regimen for PBL. The optimal therapeutic modality necessitates further exploration.
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- 2021
11. Prognostic model and immune-infiltrating cell landscape based on differentially expressed autophagy-related genes in TP53-mutated multiple myeloma
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Hong-Yuan Shen, Guangxun Gao, Yan-Hua Zheng, Xiang Chen, and Juan Feng
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business.industry ,Cell ,Autophagy ,General Medicine ,Tp53 mutation ,medicine.disease ,medicine.anatomical_structure ,Immune system ,medicine ,Cancer research ,Prognostic model ,business ,Gene ,Multiple myeloma - Abstract
IntroductionAutophagy functions as a prosurvival mechanism in multiple myeloma (MM).The objective of this research was to establish an autophagy-related gene (ARG) signature for predicting the survival outcomes of MM patients with TP53 mutations.Material and methodsInformation about MM patients with TP53 mutations was downloaded from Gene Expression Omnibus (GEO) database. Cox proportional hazard regression was employed to determine the independent prognostic ARG and construct a risk signature. Time-dependent receiver-operating characteristic (t ROC) curve was used to explore the predictive accuracy of the prognostic model. A nomogram was constructed to give a more precise prediction of the probability of 5-year, 8-year and 10-year overall survival (OS). In addition, we utilized the CIBERSORT algorithm to explore the distribution difference of 22 immune-infiltrating cells.ResultsThree differentially expressed ARGs (CASP8, MAPK8, RB1CC1) were finally incorporated to construct the risk model. Area under the curve (AUC) of corresponding tROC curve for 5-year,8-year and 10-year OS were 0.735, 0.686 and 0.662, respectively. MM patients were categorized into high and low-risk group in accordance with the median threshold value (-1.724549). ARG-based risk score model was an independent prognostic element correlated with OS, giving an hazard ratio (HR) of 3.29 (95%CI 2.35-4.60, PConclusionsWe established a three-ARGs risk signature which manifested an independent prognostic factor. The nomogram was testified to perform well in forecasting the long-term survival of TP53-mutated MM patients.
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- 2021
12. Rituximab-based combination therapy in patients with Waldenström macroglobulinemia: a systematic review and meta-analysis
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Mi-Mi Shu, Juan Feng, Xiequn Chen, Hailong Tang, Li Xu, Yan-Hua Zheng, Guangxun Gao, and Chun Cao
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0301 basic medicine ,Chemotherapy ,medicine.medical_specialty ,Combination therapy ,business.industry ,Anemia ,medicine.medical_treatment ,Waldenstrom macroglobulinemia ,Neutropenia ,medicine.disease ,Gastroenterology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Meta-analysis ,Internal medicine ,medicine ,Pharmacology (medical) ,Rituximab ,business ,Adverse effect ,medicine.drug - Abstract
Background To evaluate the efficacy and safety of rituximab-based combination therapy for Waldenstrom macroglobulinemia (WM), we conducted this meta-analysis by pooling the rates of overall response, major response, complete response, and grade ≥3 hematological adverse events. Methods and materials We searched for relevant studies in the databases of PubMed, Web of Science, Embase, and the Cochrane Library. The qualitative assessment of all the included articles was conducted with reference to the Newcastle-Ottawa Scale. A random-effects model was selected to perform all pooled analyses. Results We identified altogether 22 studies with a total of 806 symptomatic WM patients enrolled. The pooled analysis indicated that the rituximab-based combination therapy achieved an overall response rate (ORR) of 84% (95% CI: 81%-87%), a major response rate (MRR) of 71% (95% CI: 66%-75%), and a complete response rate (CRR) of 7% (95% CI: 5%-10%). Rituximab plus conventional alkylating agents-containing chemotherapy (subgroup A) yielded an ORR of 86% (95% CI: 81%-89%), an MRR of 74% (95% CI: 69%-79%), and a CRR of 8% (95% CI: 4%-14%). Rituximab plus purine analog (subgroup B) resulted in an ORR of 85% (95% CI: 79%-89%), an MRR of 74% (95% CI: 66%-81%), and a CRR of 9% (95% CI: 4%-15%). Rituximab plus proteasome inhibitor (subgroup C) resulted in an ORR of 86% (95% CI: 81%-90%), an MRR of 68% (95% CI: 58%-77%), and a CRR of 7% (95% CI: 3%-11%). Rituximab plus immunomodulatory drug (subgroup D) attained relatively lower response rates, with an ORR of 67% (95% CI: 51%-81%), an MRR of 56% (95% CI: 27%-83%), and a CRR of 5% (95% CI: 1%-12%). Common grade ≥3 hematological adverse events consisted of neutropenia (33%, 95% CI: 17%-52%), thrombocytopenia (7%, 95% CI: 3%-11%), and anemia (5%, 95% CI: 3%-9%). Conclusion Rituximab in combination with an alkylating agent, purine analog, or proteasome inhibitor is highly effective with tolerable hematological toxicities for WM.
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- 2019
13. Correlations between serum hepatitis B surface antigen and hepatitis B core antibody titers and liver fibrosis in treatment-naïve CHB patients
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Xing-Li Sun, Lan-xia Qie, Yan-Hua Zheng, Yun-yan Liu, Shun-mao Ma, Huanwei Zheng, De-hua Wang, Er-Hei Dai, Gui-fang Ren, Jun-Qing Li, Min-Ran Li, and Yu-Ling Wang
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Adult ,Liver Cirrhosis ,Male ,medicine.medical_specialty ,HBsAg ,Hepatitis B virus ,Liver fibrosis ,Hepatitis b surface antigen ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Hepatitis B, Chronic ,Fibrosis ,Internal medicine ,Medicine ,Humans ,Hepatitis B Antibodies ,lcsh:R5-920 ,Hepatitis B Surface Antigens ,Nucleoside analogue ,business.industry ,virus diseases ,General Medicine ,Middle Aged ,medicine.disease ,Hepatitis B Core Antigens ,Hepatitis b core antibody ,digestive system diseases ,Titer ,Cross-Sectional Studies ,Logistic Models ,HBeAg ,Liver ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Female ,business ,lcsh:Medicine (General) ,medicine.drug - Abstract
Background: Previous studies have revealed that quantitative hepatitis B surface antigen (HBsAg) or hepatitis B core antibody (qAnti-HBc) levels can be used as predictors of treatment response in both interferon-α and nucleoside analogue therapies. Few data have been published regarding the relationship between quantitative HBsAg or Anti-HBc levels and liver fibrosis stages in patients with chronic hepatitis B (CHB). Methods: We conducted a cross-sectional study of treatment-naïve CHB patients. A total of 624 CHB patients were recruited. We assessed the serum HBsAg and qAnti-HBc levels, HBV DNA levels, HBV genotypes, BCP/PC mutations, histological fibrosis staging by Scheuer classification. Results: In HBeAg (+) patients, the S0-1 subjects had significantly higher serum HBsAg and lower qAnti-HBc levels than the S2-4 subjects (both p 0.05). Serum qAnti-HBc levels showed a moderate positive correlation with fibrosis scores (r = 0.383, p
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- 2018
14. An insight into new strategies to combat antifungal drug resistance
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Yi Ding, Yan-Hua Zheng, Guangxun Gao, Yue-Yun Ma, and Xiequn Chen
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0301 basic medicine ,Antifungal ,Antifungal Agents ,Combination therapy ,medicine.drug_class ,030106 microbiology ,Antifungal drug ,Pharmaceutical Science ,Hsp90 ,Review ,Microbial Sensitivity Tests ,Drug resistance ,Bioinformatics ,Trehalose biosynthesis ,03 medical and health sciences ,Drug Resistance, Fungal ,Drug Discovery ,medicine ,Humans ,HSP90 Heat-Shock Proteins ,Pharmacology ,Sphingolipids ,business.industry ,Calcineurin ,Fungi ,Computational Biology ,Trehalose ,carboxymethyl chitosan ,Carboxymethyl-chitosan ,Clinical evidence ,chromogranin A-N46 ,business ,Echinocandins - Abstract
Invasive fungal infections especially in immunocompromised patients represent a dominating cause of mortality. The most commonly used antifungal agents can be divided into three broad categories, including triazoles, echinocandins and polyenes. Antifungal resistance is on the increase, posing a growing threat to the stewardship of immunocompromised patients with fungal infections. The paucity of currently available antifungals leads to the rapid emergence of drug resistance and thus aggravates the refractoriness of invasive fungal infections. Therefore, deep exploration into mechanisms of drug resistance and search for new antifungal targets are required. This review highlights the therapeutic strategies targeting Hsp90, calcineurin, trehalose biosynthesis and sphingolipids biosynthesis, in an attempt to provide clinical evidence for overcoming drug resistance and to form the rationale for combination therapy of conventional antifungals and agents with novel mechanisms of action. What's more, this review also gives a concise introduction of three new-fashioned antifungals, including carboxymethyl chitosan, silver nanoparticles and chromogranin A-N46.
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- 2018
15. Primary lymphoma of bone: a population-based study of 2558 patients
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Chun Cao, Yan-Hua Zheng, Guangxun Gao, Chen-Xin Liu, Li Xu, Pan-Pan Wang, and Tian-Qi Xu
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,business.industry ,lcsh:RC633-647.5 ,Hematology ,lcsh:Diseases of the blood and blood-forming organs ,Malignancy ,medicine.disease ,survival ,SEER ,Population based study ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Primary lymphoma ,medicine ,therapeutic modality ,primary lymphoma of bone ,prognosis ,business ,Original Research - Abstract
Background: Primary lymphoma of bone (PLB) is an extremely rare malignancy arising in the skeletal system. There is no consensus over the best definition of PLB. Most of the published articles are single-institutional retrospective studies with a limited sample size. The rarity of PLB and discrepancies on diagnostic criteria has resulted in a vague understanding of PLB. Methods We retrospectively analyzed the clinical characteristics and prognostic factors of 2558 PLB patients who were registered in the Surveillance, Epidemiology, and End Results (SEER) database from 1973 to 2016. Survival rates were calculated using the Kaplan–Meier method. The effects of various factors on survival outcomes were analyzed by using the log-rank test. Univariate and multivariate analyses were conducted by using the Cox proportional hazards model to determine independent prognostic factors. Results: The median follow-up time of all eligible patients was 58 months. There seemed no sex preponderance in PLB incidence. The most involved sites are axial skeletons. The most common histological subtype was diffuse large B-cell lymphoma. The 3-, 5-, 10-, and 20-year overall survival (OS) rates were 70.70%, 65.70%, 54.40% and 39.50%, respectively. PLB patients whose primary tumor sites were appendicular and craniofacial skeletons had a significant survival advantage [hazard ratio (HR) = 0.694, 95% confidence interval (CI) 0.552–0.872; HR = 0.729, 95% CI 0.597–0.889, respectively] over those with axial skeletons as primary tumor sites. Patients with Hodgkin lymphoma, non-Hodgkin lymphoma (NHL)–mature B-cell lymphoma, and NHL-precursor-cell lymphoblastic lymphoma also had a significant OS advantage (HR = 0.392, 95% CI 0.200–0.771; HR = 0.826, 95% CI 0.700–0.973; and HR = 0.453, 95% CI 0.223–0.923, respectively). Patients with Ann Arbor stage III–IV at diagnosis were at higher risk of death than those with stage I–II (HR = 1.348, 95% CI 1.107–1.641). Chemotherapy was an independent favorable prognostic factor (HR = 0.734, 95% CI 0.605–0.890). Conclusions: Primary anatomic site, histology type, higher Ann Arbor stage and chemotherapy were independent prognostic factors. Chemotherapy played a pivotal role in PLB treatment.
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- 2020
16. The serum galectin-3 levels are associated with the severity and prognosis of ischemic stroke
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Jia-Jun Zhuang, Yan-Hua Zheng, Li Zhou, and Yan-Sheng Ding
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Male ,Aging ,medicine.medical_specialty ,acute ischemic stroke ,Multivariate analysis ,Galectins ,Logistic regression ,Severity of Illness Index ,Internal medicine ,galectin-3 ,Medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Stroke ,Aged ,Ischemic Stroke ,Chinese ,business.industry ,Cell Biology ,Odds ratio ,Blood Proteins ,Middle Aged ,medicine.disease ,Prognosis ,Confidence interval ,Standard error ,Quartile ,ROC Curve ,Multivariate Analysis ,Female ,business ,Research Paper - Abstract
Galectin-3, a microglia/macrophage-derived inflammatory mediator, plays a role in the stroke progression. In this single-center prospective study, we included 288 consecutive patients with a first-ever acute ischemic stroke to assess the association between galectin-3 serum level and clinical severity at admission and outcome at discharge by univariate and multivariate logistic regression. The results were presented as odds ratios (OR) and 95% confidence intervals (CI). Patients with high severity and poor outcomes had higher serum levels of galectin-3 (P
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- 2020
17. Pulmonary Lymphomatoid Granulomatosis With Hemophagocytic Lymphohistiocytosis as the Initial Manifestation
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Xuan Zhang, Li Xu, Yan-Hua Zheng, Ying-Juan Lu, and Guangxun Gao
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0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lymphomatoid granulomatosis ,Hepatosplenomegaly ,lymphomatiod granulomatosis ,Case Report ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,rare lymphoma ,hemic and lymphatic diseases ,Medicine ,Epstein-Barr virus ,Hemophagocytic lymphohistiocytosis ,Lung ,business.industry ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Pancytopenia ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,hemophagocytic lymphohistiocytosis ,030220 oncology & carcinogenesis ,pathology ,Pulmonary Lymphomatoid Granulomatosis ,Hemophagocytosis ,medicine.symptom ,business ,Vasculitis - Abstract
Lymphomatoid granulomatosis (LYG) is an extremely rare angio-centric and angio-destructive B-cell lymphoproliferative disease. Driven by Epstein-Barr virus (EBV), LYG predominantly involves the bilateral lungs. Commonly presenting as multiple nodules in the lung, pulmonary LYG can masquerade as various infectious diseases, vasculitis, lung cancer, or other metastatic neoplasm. It is difficult to be diagnosed and is always neglected by clinicians. No standardized therapeutic regimens for LYG has been established yet now. Hemophagocytic lymphohistiocytosis (HLH), a life-threatening condition caused by abnormal activation of macrophages and T-cells, is characterized by fever, hepatosplenomegaly, pancytopenia, hypercytokinemia, and the presence of hemophagocytosis within the bone marrow, liver, spleen, or other lymphatic tissue. We herein report a 55-year-old man with recurrent fever, severe jaundice, and multiple high-density opacities and nodules in both lungs, who was finally diagnosed with pulmonary LYG (Grade 3) manifested with secondary HLH. Administration of HLH-1994 protocol led to the rapid control of the symptoms caused by HLH. Rituximab-based combination therapy was useful yet LYG (Grade 3) progressed rapidly. This case demonstrates that tissue biopsy is essential for early pathological diagnosis and effective treatment of LYG.
- Published
- 2020
18. [Effect of Jianpi Shengxue Granule on Fertility and Early Embryo Development Toxicity of Rats]
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Wen, Qu, Zhi-Chun, Jin, Gang, Zhao, Qi-Zhi, Yu, Yan-Hua, Zheng, Qin-Qi, Liu, and Huang Zhi-Jun
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Epididymis ,Male ,Rats, Sprague-Dawley ,Fertility ,Body Weight ,Sperm Motility ,Animals ,Embryonic Development ,Female ,Organ Size ,Spermatozoa ,Drugs, Chinese Herbal ,Rats - Abstract
Objective To assess the effects of Jianpi Shengxue Granule (JSG) on the fertility and early embryo development in rats. Methods Totally 176 SD rats were stratified by sex, and divided into 4 groups, i.e., the control group, low, medium, high dose JSG treatment groups. Rats in the 3 JSG treatment groups were administered with JSG at 0, 1. 16, 3. 48, 5. 80 g/kg per day by gastrogavage, re- spectively. After 2 weeks of administration for females and 4 weeks of administration for males, males and females were caged in the ratio of 1:1. Females were administered to the gestation day 7. Males were administered for 59 -63 days until the day before anatomy. All parental generations were anatomized to observe signs and morphologies. Pathological examination was performed. General toxicity was detected. The testis and epididymis were weighed. Spermatozoa number was counted from epididymis, and repro- duction toxicity of sperm motility was checked. The numbers of corpus luteum, live fetus, dead fetus, and absorbed fetus were counted. The implantation number was calculated to observe early embryo de- velopment toxicity. Results (1) General toxicity: The body weight growth slowed down in male rats of the high dose JSG treatment group. No abnormality was found in low and medium dose JSG treatment groups. (2)Fertility toxicity: There was no obvious toxic effect on testis, epididymis, and spermatozoa number from epididymis sperm in all JSG treatment groups. (3) Early embryo development toxicity: No significant effect on the formation of early embryos was found in all JSG treatment groups. Conclusions Certain gastrointestinal toxicity of JSG might exist to some extent. The dose for non-adverse effect of JSG on fertility and early embryo development was 3. 38 g/kg per day. No obvious fertility or early embryo development toxicity occurred in each JSG treatment group.
- Published
- 2019
19. Relationship of the polymorphism of rs10795668 single nucleotide and the susceptibility of colorectal cancer in north China Han population
- Author
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Kai FENG, Hao-jun LU, Hai-ying JIA, Yan-hua ZHENG, Jun ZHANG, Qing-ping JING, and Ji-hua WU
- Subjects
lcsh:R5-920 ,single nucleotide polymorphism ,lcsh:R ,lcsh:Medicine ,colorectal cancer ,lcsh:Medicine (General) ,genetic susceptibility - Abstract
Objective To evaluate the relationship between the rs10795668 single nucleotide polymorphism (SNP) and the colorectal cancer (CRC) in north China Han population. Methods The genotype of rs10795668 SNP was detected by PCRRFLP analysis in 113 colon cancer patients, 69 rectal cancer patients, and 192 age and sex matched controls. Unconditional logistic regression were used to comparatively analyze the genotype between patients and the controls adjusted by age and sex. Results No significant difference of rs10795668 SNP was detected between colorectal cancer patients and normal controls, but it was found that an existence of interaction of gene and sex in colorectal cancer, and women with AA+AG genotype may have lower risk of colon cancer (OR3.05, 95% CI 1.47-6.35; P=0.00078). Conclusion The AA+AG genotype of rs10795668 SNP is associated with the genetic susceptibility of colon cancer, but not of rectal cancer, in north China Han women. DOI: 10.11855/j.issn.0577-7402.2015.10.10
- Published
- 2015
20. BRD4 inhibitor nitroxoline enhances the sensitivity of multiple myeloma cells to bortezomib in vitro and in vivo by promoting mitochondrial pathway-mediated cell apoptosis
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Juan Feng, Cheng Luo, Yan-Hua Zheng, Yan-Ping Song, Hailong Tang, Xiequn Chen, Li Xu, and Guang Li
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0301 basic medicine ,BRD4 ,lcsh:RC633-647.5 ,Bortezomib ,business.industry ,lcsh:Diseases of the blood and blood-forming organs ,Hematology ,medicine.disease ,In vitro ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Nitroxoline ,chemistry ,In vivo ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,medicine ,Hematological neoplasm ,business ,Multiple myeloma ,medicine.drug - Abstract
Background: Multiple myeloma (MM) is the second most common hematological neoplasm. Wide administration of bortezomib significantly improves the survival of MM patients compared with conventional chemotherapy. Bromodomain-containing protein 4 (BRD4) inhibitors also have been demonstrated to retard cell proliferation and induce cellular apoptosis in various cancers. However, it is unclear whether the BRD4 inhibitor nitroxoline plus bortezomib has a synergistic anti-tumor effect on MM. Methods: Cell viability was determined via 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle and cell apoptosis were assessed via flow cytometry. Protein expression levels were determined via western blotting. The expression of apoptosis-related proteins in xenograft tissue were detected by means of immunohistochemistry. Results: Treatment with nitroxoline or bortezomib suppressed cell proliferation, and caused G0/G1 phase arrest and apoptosis in H929 and RPMI8226 cells. Furthermore, nitroxoline intensified the retardation of cell proliferation, as well as further enhanced the G0/G1 phase arrest and apoptosis induced by bortezomib in H929 and RPMI8226 cells. The western blot analysis revealed that nitroxoline or bortezomib treatment markedly diminished the levels of Bcl-2 and cyclin D1, and increased the levels of p21, Bax, cleaved PARP and cleaved caspase-3. Combination of these two agents was observed to result in further marked changes on these levels compared with nitroxoline or bortezomib treatment alone. What is more, in the xenograft tumor model, combinative treatment markedly inhibited tumor growth compared with the single drug treatment. Conclusion: Combination of bortezomib with nitroxoline has a synergistic anti-tumor activity in MM cells and may be a novel treatment method for MM.
- Published
- 2020
21. Monoclonal Antibodies versus Histone Deacetylase Inhibitors in Combination with Bortezomib or Lenalidomide plus Dexamethasone for the Treatment of Relapsed or Refractory Multiple Myeloma: An Indirect-Comparison Meta-Analysis of Randomized Controlled Trials
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Hong-Yuan Shen, Na Zhang, Hailong Tang, Juan Feng, Guangxun Gao, Xiequn Chen, Yan-Hua Zheng, and Li Xu
- Subjects
Oncology ,lcsh:Immunologic diseases. Allergy ,medicine.medical_specialty ,Combination therapy ,Immunology ,Review Article ,Dexamethasone ,Disease-Free Survival ,Bortezomib ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Immunology and Allergy ,Humans ,Lenalidomide ,Multiple myeloma ,Randomized Controlled Trials as Topic ,business.industry ,Daratumumab ,Antibodies, Monoclonal ,General Medicine ,medicine.disease ,Combined Modality Therapy ,Thalidomide ,Histone Deacetylase Inhibitors ,Treatment Outcome ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Neoplasm Recurrence, Local ,business ,Multiple Myeloma ,lcsh:RC581-607 ,Progressive disease ,030215 immunology ,medicine.drug - Abstract
During the past decades, agents with novel mechanisms of action, such as monoclonal antibodies (MAbs) and histone deacetylase inhibitors (HDACis) have been applied to treat relapsed or refractory multiple myeloma (RRMM). The treatment outcomes of MAbs versus HDACi in combination with bortezomib or lenalidomide plus dexamethasone remain unknown. We conducted this meta-analysis to compare indirectly the efficacy and safety of MAbs and HDACis in combination with bortezomib or lenalidomide plus dexamethasone. Six trials (eight articles) were included in the meta-analysis with 3270 RRMM patients enrolled. We synthesized hazard ratios (HRs) for progression-free survival (PFS) and overall survival (OS), risk ratios (RRs) for complete response (CR),very good partial response (VGPR), overall response (OR), progressive disease plus stable disease (PD + SD) and common at least grade 3 adverse events, and their corresponding 95%confidence intervals (95% CI). Treatment with MAbs in combination with bortezomib or lenalidomide plus dexamethasone resulted in longer PFS (HR 0.83, 95% CI: 0.66–0.98), fewer incidences of at least grade 3 thrombocytopenia (RR 0.35, 95% CI: 0.23–0.53), neutropenia (RR 0.70, 95% CI: 0.51–0.96), and sense of fatigue (RR 0.37, 95% CI: 0.17–0.82) than HDACis. The daratumumab plus bortezomib or lenalidomide and dexamethasone might significantly improve PFS in comparison with HDACis plus bortezomib or lenalidomide and dexamethasone (HR 0.55, 95% CI: 0.40–0.74). In conclusion, MAbs may be superior to HDACis in achieving longer PFS and may be better tolerated when in combination therapy with bortezomib or lenalidomide plus dexamethasone.
- Published
- 2018
22. Association between the single nucleotide polymorphism of RASSF1A Ala133Ser and colorectal cancer in north Chinese Han population
- Author
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Kai FENG, Hao-jun LU, Hai-ying JIA, Yan-hua ZHENG, Jun ZHANG, Qing-ping JING, and Ji-hua WU
- Subjects
endocrine system ,lcsh:R5-920 ,Ras association domain family 1A gene (RASSF1A) ,lcsh:R ,polymorphism, single nucleotide ,lcsh:Medicine ,colorectal neoplasms ,lcsh:Medicine (General) - Abstract
Objective To evaluate the association between RASSF1A Ala133Ser single nucleotide polymorphism (SNP) and colorectal cancer (CRC) in patients of Han population in north China. Methods RASSF1A SNP was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 109 colon cancer (CC) patients, 67 rectal cancer (RC) patients and 189 age and sex matched healthy people (as controls). Unconditional logistic regression was used to analyze the gene-disease association and gene-sex interaction. Results The RASSF1A Ala133Ser SNP in CRC patients had no significant difference with that in normal control samples, but the interaction of genotype and sex existed in rectal cancer, Ala/Ser+Ser/Ser genotypes of RASSF1A gene increased the risk of rectal cancer in women (OR=3.78, 95%CI 1.31-10.89). Conclusion The RASSF1A 133Ser allele is associated with rectal cancer in women, but not colon cancer. DOI: 10.11855/j.issn.0577-7402.2015.03.10
- Published
- 2015
23. The inflammatory cytokine profile of myelodysplastic syndromes
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Xiequn Chen, Yan-Hua Zheng, Li Xu, Chun Cao, Shi Xin, and Bao-Xia Dong
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Oncology ,medicine.medical_specialty ,business.industry ,Myelodysplastic syndromes ,Cytokine profile ,MEDLINE ,General Medicine ,Disease ,Cochrane Library ,medicine.disease ,Proinflammatory cytokine ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Meta-analysis ,Internal medicine ,medicine ,030212 general & internal medicine ,business - Abstract
Background Accumulating evidence has indicated that the dysregulation of immunological environment has an important role in the pathogenesis of myelodysplastic syndromes (MDS). The previous studies about the levels of the inflammatory cytokines in MDS, such as TNF-α, IFN-γ, IL-6, IL-8, and IL-17, have yielded controversial results. Thus, we performed a meta-analysis to assess the levels of these inflammatory cytokines in MDS. Methods A systematic search in PubMed, MEDLINE, Cochrane Library, Web of Science, CNKI, and CBM was conducted to find eligible studies. Meta-analyses were performed using STATA 12.0 for Windows. Heterogeneity between included studies was assessed by I test. We chose SMD as the summary statistic. Results A total of 697 individuals from 11 studies were included in this study. Our results suggest the levels of TNF-α, IL-6, IL-8 were significantly higher in MDS patients compared with controls, SMD and 95%CI was 1.48 (0.60, 2.36), 0.71 (0.16, 1.25) and 0.69 (0.28, 1.09), respectively. Moreover, the levels of IL-17 have decreased in the high-risk MDS, the SMD and 95% CI was 2.96 (0.78, 5.15). Conclusion A close association between immunological microenvironment disorders and the pathogenesis of MDS was revealed in this meta-analysis. More importantly, the profiles of inflammatory cytokines appear to change along the progression of the disease.
- Published
- 2019
24. BRD4 inhibitor nitroxoline enhances the sensitivity of multiple myeloma cells to bortezomib in vitro and in vivo by promoting mitochondrial pathway-mediated cell apoptosis.
- Author
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Guang Li, Yan-Hua Zheng, Li Xu, Juan Feng, Hai-Long Tang, Cheng Luo, Yan-Ping Song, and Xie-Qun Chen
- Published
- 2020
- Full Text
- View/download PDF
25. A Research on Key Management in Wireless Sensor Networks
- Author
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Yan Hua Zheng
- Subjects
Key distribution in wireless sensor networks ,Engineering ,Wi-Fi array ,Scope (project management) ,business.industry ,Wireless network ,Mobile wireless sensor network ,General Medicine ,Key management ,business ,Wireless sensor network ,Sensor web ,Computer network - Abstract
With the constant development of wireless sensor networks technology, the expanding application scope and the increasing processing power of sensor nodes, there are more and more data collected and processed through the sensor, thus ensuring the security of information transmission between nodes is in urgent needs. The secure application of wireless sensor networks has become a hot technology. This paper mainly expounds the key management schemes of wireless sensor networks, and analyzes the mainstream key management schemes.
- Published
- 2013
26. Different Activities of Osteoblast and Bacteria on a Nanostructured Titanium Surface for Dental Implant
- Author
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Yan Hua Zheng, Jin Bo Li, Xuanyong Liu, and Jiao Sun
- Subjects
Materials science ,biology ,medicine.medical_treatment ,General Engineering ,chemistry.chemical_element ,Osteoblast ,engineering.material ,biology.organism_classification ,Streptococcus mutans ,medicine.anatomical_structure ,chemistry ,Coating ,medicine ,engineering ,MTT assay ,Dental implant ,Antibacterial activity ,Bacteria ,Titanium ,Nuclear chemistry - Abstract
Insufficience of osteogenesis and antimicrobial effect have been still impacted the long term clinical success rate of dental implants. A nanostructured titanium surface prepared by hydrothermal treatment with H2O2 was evaluated on its osteoblastic viability and antibacterial effect. Samples were divided into 2 groups: untreated pure titanium surface (Ti) and a nanostructured titanium surface (NT). The antibacterial activities against S.mutans and C.albicans were measured by film applicator coating assay, as well as the live/dead bacteria stain. The osteoblastic viability was investigated by SEM and MTT assay. Results showed that the active microbia on NT was reduced at 24h (P
- Published
- 2012
27. [Chronic toxicity test of fangyouling by transdermal administration in rabbits]
- Author
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Zi-ming, Jia, Miao-miao, Yue, Yan-hua, Zheng, Jin-ming, Zhang, and Li-jun, Tang
- Subjects
Male ,Dose-Response Relationship, Drug ,Body Weight ,Animal Structures ,Administration, Cutaneous ,Schistosomicides ,Models, Animal ,Animals ,Humans ,Schistosomiasis ,Female ,Rabbits ,Toxicity Tests, Chronic ,Drugs, Chinese Herbal - Abstract
To observe the toxicity of fangyouling after one month' s transdermal administration in rabbits and evaluate its security.Forty rabbits were randomly divided into 4 groups including a control group and low, middle and high dose groups of fangyouling. The rabbits in the control group were administered with sunflower oil, and the other rabbits were administrated dermally with fangyouling of 50,300 and 2,000 mg/kg respectively once a day for 4 weeks. The general condition, the skin irritation reaction, body weight, food consumption, hematology, blood biochemistry, organ coefficients and histopathological changes of all the rabbits were observed.There was no obvious effect on the general condition in all the rabbits. However, the mild skin irritation was observed in 2 rabbits of the middle dose group and 4 rabbits of the high-dose group. The decreases of body weight and food consumption were noted in the high dose group. No changes were detected of hematology, blood biochemistry or viscera pathological at all dose levels.The dose of non-toxic response of fangyouling is 50 mg/kg at this study condition.
- Published
- 2015
28. [Effect of low-frequency electroacupuncture intervention on oxidative stress and glucose metabolism in rats with polycystic ovary syndrome]
- Author
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Yan-Hua, Zheng, Tao, Ding, Dan-Feng, Ye, Hua, Liu, Mao-Hua, Lai, and Hong-Xia, Ma
- Subjects
Blood Glucose ,Rats, Sprague-Dawley ,Oxidative Stress ,Electroacupuncture ,Malondialdehyde ,Animals ,Humans ,Insulin ,Female ,Acupuncture Points ,Polycystic Ovary Syndrome ,Rats - Abstract
To observe the effect of low-frequency electroacupuncture (EA) treatment on oxidative stress and metabolism disorder in polycystic ovary syndrome (PCOS) rats, so as to study its underlying mechanisms in improving PCOS.Thirty female SD rats were randomized into control group, model group and EA group (n = 10 in each group). The PCOS model was established by subcutaneous injection of testosterone propionate (1 mg/100 g, 10 mg/mL) for 8 weeks. EA (2 Hz) was applied to "Zhongwan" (CV 12) , "Guanyuan" (CV 4) and bilateral "Sanyinjiao" (SP 6) or "Housanli" (ST 36) for 30 min, once daily (except weekends) for 5 weeks. Serum testosterone (T) , sex hormone-binding globulin (SHBG) and fasting serum insulin (FINS) contents were detected by ELISA, serum malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assayed by thiobarbituric acid (TBA) and xanthine oxidation (XTO) methods, respectively, and fasting blood glucose (FBG) was determined by roche glucose meter. In addition, homeostasis model assessment for insulin resistance (HOMA-IR) and modified B cell function index (MBCI) were calculated.Compared with the control group, serum T, FBG, FINS, HOMA-IR, and serum MDA levels were significantly increased in the model group (P0.01), while serum SHBG, MBCI and SOD levels were considerably decreased in the model group (P0.01). Following EA treatment, all the increased serum T, FBG, FINS, HOMA-IR, and serum MDA levels, and the decreased serum SHBG, MBCI and SOD levels were reversed obviously (P0.05, P0.01).EA treatment may normalize insulin sensitivity, ameliorate insulin resistance and hyperinsulinemia in PCOS rats, probably by regulating the function of pancreatic islets β cell and by reducing oxidative stress and free androgen.
- Published
- 2015
29. [Effects of hypergravity exposure after 30 days of simulated weightlessness on chemokine CCL20 and its receptor CCR6 in gingival tissue of rhesus macaque]
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Chu-hua, Tang, Zhong-ying, Niu, Yan-hua, Zheng, Bo, Bao, Biao, Hong, and Yu-hui, Chen
- Subjects
Male ,Receptors, CCR6 ,Chemokine CCL20 ,Weightlessness ,Gingiva ,Animals ,Hypergravity ,RNA, Messenger ,Macaca mulatta - Abstract
To study the effect of hypergravity exposure after 30 days of simulated weightlessness on the expression of chemokine CCL20 and its receptor CCR6 in gingival tissue of rhesus macaque.Twenty-three male rhesus monkeys were randomly divided into 4 groups, namely control group (A,n=3), weightlessness group (B,n=3), hypergravity group (C,n=3) and hypergravity exposure after 30 days of simulated weightlessness group (D, n=14). Group D was further divided into 4 subgroups according to the values of overload as: +11 Gx /270 s group (D1, n=3), +13 Gx /230 s group (D2,n=4), +15 Gx/200 s group (D3,n=4) and +13 Gx /230 s with 9 days of recovery group (D4, n=3). Histopathological changes of gingival tissues were observed by hematoxylin-eosin staining and the expressions of CCL20 and CCR6 were detected by immunohistochemistry (IHC) and quantitative real-time PCR (Q-PCR). SPSS 17.0 software package was used for statistical analysis.Histological observation showed that no significant histopathological change was found in the gingival tissues in all experimental groups. However, there were more infiltrated lymphocytes and neutrophils in the experimental groups. Normal gingival epithelial cells were hardly stained by anti-CCL20 but weakly stained by anti-CCR6. In the experimental groups, CCL20 could be detected in the basal layer of the gingival epithelial tissue, and CCR6 could be detected in the spinous layer and the basal layer of the gingival epithelium. The CCL20 and CCR6 expression in the gingival tissues of each experimental group were significantly higher than those of the control group, not only at the protein level but at the mRNA level (P0.05) except the CCL20 expression in the weightlessness group.Hypergravity exposure after 30 days of simulated weightlessness will not lead to significant pathological changes in gingival tissues, but can induce the expression of chemokine CCL20 and its receptor CCR6 in gingival tissue.
- Published
- 2014
30. [Effect of Yangjing Zhongyu Decoction on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulosa cells cultured by excess androgen]
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Yan-Hua, Zheng, Tao, Ding, Hong-Xia, Ma, Dan-Feng, Ye, Nian-Jun, Su, and Xiao-Ke, Wu
- Subjects
Granulosa Cells ,Ovarian Follicle ,Swine ,Proliferating Cell Nuclear Antigen ,Androgens ,Animals ,Membrane Transport Proteins ,Receptors, FSH ,Female ,RNA, Messenger ,Follicle Stimulating Hormone ,Cells, Cultured ,Drugs, Chinese Herbal - Abstract
To observe the effect of Yangjing Zhongyu Decoction (YZD) on mRNA and protein expression of PCNA, StAR, and FSHR in ovarian granulose cells (GCs) cultured by excess androgen.Ovarian GCs from porcine follicles were isolated and cultured in vitro. Follicular stimulating hormone (FSH) or YZD was added in the GCs treated by excess testosterone propionate. Totally 48 h later mRNA and protein expression of PCNA, StAR, and FSHR were detected by RT-PCR and Western blot.Excess androgen inhibited mRNA and protein expression of PCNA, StAR, and FSHR of GCs. FSH and YZD could antagonize inhibition of excess androgens, and promote mRNA and protein expression of PCNA, StAR, and FSHR in GCs.YZD could antagonize the inhibition of excess androgen on mRNA and protein expression of PCNA, StAR and FSHR in GCs. Thus, we inferred that YZD could improve the follicle dysplasia by promoting mRNA and protein expression of PCNA, StAR and FSHR in GCs.
- Published
- 2014
31. [Treatment of PCOS by acupuncture: present experimental and clinical reports]
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Yan-hua, Zheng, Nian-jun, Su, and Tao, Ding
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Acupuncture Therapy ,Animals ,Humans ,Female ,Polycystic Ovary Syndrome - Published
- 2012
32. Quantitative hepatitis B core antibody level is associated with inflammatory activity in treatment-naïve chronic hepatitis B patients
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Min-Ran Li, Yan-Hua Zheng, Xing-Li Sun, Li-hong Ye, Zhi-quan Liu, Er-Hei Dai, Hai-cong Zhang, Yan Huang, Ying Lv, Jian-hua Lu, and Yun-yan Liu
- Subjects
Male ,ALT ,viruses ,Severity of Illness Index ,Gastroenterology ,0302 clinical medicine ,Hepatitis B e Antigens ,Young adult ,quantitative anti-HBc ,virus diseases ,Alanine Transaminase ,General Medicine ,Middle Aged ,Hepatitis B ,liver inflammation ,Hepatitis B Core Antigens ,HBeAg ,030220 oncology & carcinogenesis ,Predictive value of tests ,Female ,030211 gastroenterology & hepatology ,medicine.symptom ,Research Article ,medicine.drug ,Adult ,Hepatitis B virus ,medicine.medical_specialty ,Genotype ,Observational Study ,Inflammation ,Young Adult ,03 medical and health sciences ,Hepatitis B, Chronic ,Chronic hepatitis ,Predictive Value of Tests ,Internal medicine ,Severity of illness ,medicine ,Humans ,chronic hepatitis B ,Aspartate Aminotransferases ,Hepatitis B Antibodies ,Nucleoside analogue ,business.industry ,medicine.disease ,digestive system diseases ,ROC Curve ,Immunology ,business - Abstract
Previous studies have shown that hepatitis B core antibody (anti-HBc) levels vary during different phases of disease in treatment-naïve chronic hepatitis B (CHB) patients and can be used as a predictor of both interferon-α and nucleoside analogue therapy response. However, there is no information on the association between the quantitative serum anti-HBc (qAnti-HBc) level and liver inflammation in CHB patients. Therefore, we investigated these relationships in a large cohort of treatment-naïve CHB patients. A total of 624 treatment-naïve CHB patients were included in the study. The serum qAnti-HBc level was moderately correlated with ALT and AST levels (P
- Published
- 2016
33. [Observation on therapeutic effect of acupoint sticking at Neiguan (PC 6) and Xinshu (BL 15) for treatment of bradycardia]
- Author
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Lian-deng, Xu, Yu-xia, Zhang, and Yan-hua, Zheng
- Subjects
Male ,Treatment Outcome ,Acupuncture Therapy ,Bradycardia ,Humans ,Female ,Middle Aged ,Acupuncture Points ,Aged - Abstract
To observe the therapeutic effect of acupoint sticking of Wuzhuyu (Evodia Rutaecarpa) for treatment of bradycardia.Sixty cases were randomly divided into an observation group and a control group, 30 cases in each group. The observation group was treated with acupoint sticking of Wuzhugu (Evodia Rutaecarpa) at Neiguan (PC 6) and Xinshu (BL 15) once each day. The control group was treated with oral administration of Xinbao pills, 3 pills each time, thrice each day. The therapeutic effects and changes of 24 hours' holter were observed after 4 weeks.After treatment, 24 hours' average heart rate was significantly improved in the two groups, with significant differences as compared with those before treatment (both P0.01) and with no significant difference between the two groups (P0.05). The total effective rate was 86.7% (26/30) in the observation group and 83.3% (25/30) in the control group, their therapeutic effect being similar.Acupoint sticking of Wuzhugu (Evodia Rutaecarpa) can significantly raise the levels of 24 hours' average heart rate in the patients of bradycardia. This therapy and Xinbao pills have similar therapeutic effect on the improvement of clinical symptom and heart rate levels.
- Published
- 2010
34. [Establishment of endometriosis diagnostic model using plasma protein profiling]
- Author
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Hai-yuan, Liu, Yan-hua, Zheng, Jian-zhong, Zhang, Jin-hua, Leng, Da-wei, Sun, Zhu-feng, Liu, Lan, Zhu, and Jing-he, Lang
- Subjects
Adult ,Molecular Weight ,Proteomics ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Endometriosis ,Protein Array Analysis ,Humans ,Female ,Blood Proteins ,Models, Biological ,Sensitivity and Specificity ,Biomarkers - Abstract
To establish the diagnostic model for endometriosis by screening the plasma biomarkers of endometriosis using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)coupled with bioinformatics.Plasma samples from 36 patients with endometriosis (endometriosis group) matched with 35 patients with infertility or benign ovarian tumors (control group) before laparoscopy were collected at Peking Union Medical College Hospital from January to October 2007. Plasma protein profiling were detected using SELDI-TOF-MS and protein chip and peak intensities were compared between the two groups. Biomarker Discovery Software was used for data analysis and model was build by classification and regression tree software (CART), sensitivity and specificity of the diagnostic model were verified.There were 14 protein peaks abnormally expressed in endometriosis group compared with those of control group (P0.01). The diagnostic model composed of three protein peaks with the molecular weight of 3,956,000, 11,710,000 and 6,986,000 showed a sensitivity of 92% and specificity of 83%. In the blind test the model showed a sensitivity of 88% and specificity of 80%.SELDI-TOF-MS is a new approach for screening markers of endometriosis. Its clinical value deserves further investigation.
- Published
- 2009
35. [Study for drug-resistance of epithelial ovarian cancer by serum protein profiling]
- Author
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Wen-ying, Zhang, Li-rong, Zhu, Yan-hua, Zheng, Ling, Zhou, Jian-zhong, Zhang, Jiu-hong, Wu, and Qin-ping, Liao
- Subjects
Adult ,Ovarian Neoplasms ,Proteomics ,Drug Resistance, Neoplasm ,Case-Control Studies ,Biomarkers, Tumor ,Humans ,Female ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Blood Proteins ,Middle Aged ,Peptide Mapping ,Neoplasm Staging - Abstract
To develop the rationales for ovarian cancer-specific protein profiles in serum and to analyze the protein profiles of multidrug resistance P-glucoprotein (MDR1) and multidrug resistance-associated protein (MRP) positive and negative expression sets for a rapid and sensitive serum protein pattern.Serum protein profiles from 36 epithelial ovarian cancer cases were compared with 30 healthy controls using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (DELDI-TOF-MS). Blinded test was conducted subsequently for identification of the protein pattern. Expression of MDR and MRP were detected in set of training cases by immunohistochemistry. The spectra were analyzed statistically between positive and negative groups.Twenty-nine peaks were significantly different between ovarian cancer and healthy controls (P0.01, 15 peaks up-regulated and 14 down-regulated). A set of three peaks, at 5 486, 6 463 and 8 575 m/z respectively, were selected from 29 sense peaks as an ovarian cancer biomarker. The sensitivity was 100% and specificity 93.33%. Identification by blinded validation indicated a positive predictive value of 90%. MDR1 expression in ovarian cancer was 69.4 %. Twenty peaks were significantly different between positive and negative sets (P0.01). MRP expression in ovarian cancer was 63.8%. But one peak was detected (P0.01).It was an ideal pattern for employing the SELDI mass spectrum approach to diagnose ovarian cancer and select the multidrug resistance cases. Serum biomarkers for detecting drug resistance in ovarian cancer can be established on the basis of MDR1 immunohistochemistry.
- Published
- 2009
36. [Detection of tissue protein biomarkers by surface enhanced laser desorption/ionization-time of flight-mass spectrometry in patients with mycosis fungoides]
- Author
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Jie, Liu, Yan-Hua, Zheng, Yun-Fei, Zhou, Jian-Zhong, Zhang, and Bao-Xi, Wang
- Subjects
Adult ,Male ,Mycosis Fungoides ,Skin Neoplasms ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Humans ,Female ,Middle Aged ,Biomarkers ,Aged - Abstract
To search the tissue biomarker in mycosis fungoides (MF) patients by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS).Skin specimens were collected from 12 MF patients and 15 normal controls to prepare solution of protein. CM10 Proteinchip was used to detect the biomarkers for MF. Differentially expressed protein was identified by immuno capturing.The expression intensity levels of 24 proteins were different between the MF patients and controls. By searching in SWISS-PRO database and literature, the 3498 and 10 837 peaks were found to accord with HNP3 and S100A8 proteins respectively. By immuno capturing, 10 837 could be identified as S100A8 with a special antibody against S100A8.A quick, easy, and high throughout proteome analytic method, SELDI-TOF-MS can identify the differential expressed proteins from MF tissue, thus offering a unique platform for biomarker detection of MF.
- Published
- 2009
37. [Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides]
- Author
-
Jie, Liu, Yan-Hua, Zheng, Jian-Zhong, Zhang, and Bao-Xi, Wang
- Subjects
Mycosis Fungoides ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Biomarkers, Tumor ,Humans ,Blood Proteins - Abstract
To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.
- Published
- 2009
38. [Study of a serum protein fingerprint diagnostic model in patients with hypertensive disorder complicating pregnancy]
- Author
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Li-yun, gong, Zhen-yu, Zhang, Yan-hua, Zheng, and Jian-zhong, Zhang
- Subjects
Adult ,Pregnancy Complications ,Proteomics ,Young Adult ,Pregnancy ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Hypertension ,Protein Array Analysis ,Humans ,Female ,Blood Proteins ,Peptide Mapping - Abstract
To analyze the alterations of serum protein fingerprint in patients with hypertensive disorder complicating pregnancy (HDCP), screen serum biomarker and establish a diagnostic model of HDCP.Surface-enhanced laser desorption lionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology was used to analyze serum including 25 cases of HDCP patients and 30 cases of age-, gravity- and parity-matched healthy term pregnant women on IMAC3-Cu proteinchip before delivery. Biomarker Wizard and Biomarker Pattern software was used to detect protein peaks significantly different between HDCP and controls, and establish a primary diagnostic model of HDCP. This model was further evaluated by blind test using other 16 parts of serum protein fingerprint.Ten protein peaks were significantly different at the molecular range of 2000-50000 (P0.01). A diagnostic model consisting of 5 protein peaks(39 837,6196,15 529, 43 248 and 22 292) was established with 100% (25/25) sensitivity, 90% (27/30) specificity, 89% (25/28) positive predictive value and 100% (27/27) negative predictive value. Blind test generated a sensitivity of 75% and specificity of 75% respectively.Many differential expressed proteins exist in serum of patients with HDCP. The diagnostic model consisting of 5 protein peaks has relative high sensitivity and specificity, and can discriminate HDCP from healthy pregnant
- Published
- 2008
39. Proteiomic patterns for endometrial cancer using SELDI-TOF-MS
- Author
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Li Yu, Wen-ying Zhang, Qin-ping Liao, Li-rong Zhu, Jun Hu, and Yan-hua Zheng
- Subjects
Oncology ,Proteomics ,medicine.medical_specialty ,Pathology ,Protein Array Analysis ,Medical Oncology ,Models, Biological ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Internal medicine ,SELDI-TOF-MS ,medicine ,Biomarkers, Tumor ,Diagnostic biomarker ,Humans ,General Pharmacology, Toxicology and Pharmaceutics ,Neoplasm Staging ,Training set ,General Veterinary ,Chemistry ,Endometrial cancer ,Cancer ,General Medicine ,medicine.disease ,Serum samples ,Endometrial Neoplasms ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Biomedicine ,Case-Control Studies ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Female - Abstract
Serum samples from endometrial cancer (EC) patients and healthy females were analyzed using surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF-MS) to discover the potential diagnostic biomarker for detection of EC. A preliminary training set of spectra derived from 40 EC patients and 30 healthy women were used to develop a proteomic model that effectively discriminated cancer patients from healthy women. The training set had a specificity of 100% and sensitivity of 92.5% in the EC detection. A blind test set, including 20 new cancer cases and 10 healthy women, was used to validate the sensitivity and specificity of this multivariate model, which had a corresponding results of 60% in specificity and 75% in sensitivity, respectively. The combination of SELDI-TOF-MS with bioinformatics tools could help find new biomarkers and establish the detection of EC with high sensitivity and specificity.
- Published
- 2008
40. [The apoptosis-inducing activity of human selenoprotein P shorter isoform]
- Author
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Qing, Fang, Yao, Yi, Yan-hua, Zheng, Quan, Chen, Li, Ning, Yuan-yuan, Zha, Sheng-li, Bi, Jian-guo, Yang, and Chen, Lin
- Subjects
Male ,Mice, Inbred BALB C ,Mitochondrial Permeability Transition Pore ,Proteins ,Apoptosis ,Mitochondria, Liver ,Mitochondrial Membrane Transport Proteins ,Ion Channels ,Membrane Potentials ,Selenocysteine ,Mice ,Selenium ,Selenoprotein P ,Mutation ,Escherichia coli ,Animals ,Humans ,Protein Isoforms ,Cysteine ,Cloning, Molecular ,Selenoproteins - Abstract
Human selenoprotein P (HSelP) is unique protein that contains 10 selenocysteines encoded by 10 inframe UGA, which typically function as stop codon. The function of HSelP remains unclear, in part due to the inability to express it by gene recombinant technique. This study is to investigate expression and purification of recombinant HSelP in prokaryotic expression system, and its activity to induce apoptosis in vitro.The shorter HSelP isoform was cloned. After the selenocysteine (SeCys) at 40th position from N terminus of the HSelP shorter isoform was mutated into cysteine by PCR, it was expressed in E. coli. The expressed product was purified with DEAE column and identified by Western blot. Subsequently, its function on induction of mitochondrial apoptotic activity was studied.The mutant HSelP shorter isoform expressed in prokaryotic system was purified by DEAE column to 90% homogeneity. The purified product, HSelP280m, induced the opening of mitochondrial permeability transition pore (PTP) and decreased the transmembrane potential in a dose-dependent manner. These events could be abolished by PTP specific inhibitors.HSelP280m can induce the opening of mitochondrial PTP, which provides a basis for investigating the structure and function of recombinant HSelP.
- Published
- 2005
41. [Primary culture of human periodontal ligament fibroblasts by explants with enzymatic digestion]
- Author
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Chu-hua, Tang, Sheng-gen, Shi, Zhong-ying, Niu, Ping, Dang, Yan-hua, Zheng, and Liang, Shi
- Subjects
Periodontal Ligament ,Cell Culture Techniques ,Humans ,Fibroblasts ,Tooth - Abstract
To increase the success rate of primary culture of human periodontal ligament fibroblasts (HPLF), and to establish an experimental model for studying HPLF in vitro.The primary cells were isolated from human periodontal ligament by explants with enzymatic digestion method. Morphological analysis and immunocytochemical staining were used to characterize the cell lineage, and growth curve assay to evaluate the biological features of HPLF.The success rate of primary culture of HPLF was 77.8%. Cultured cells were spindle-shaped, and had a positive reaction to antibodies against vimentin, and a negative reaction to antibodies against keratin. Their morphological and biological characteristics were similar to those of typical HPLF. Growth of HPLF obtained by this method was satisfactory.The success rate of primary culture of HPLF is significantly increased by explants combined with enzymatic digestion. The method is simple and feasible.
- Published
- 2004
42. Identification of Serum Biomarkers for Nasopharyngeal Carcinoma by Proteomic Analysis.
- Author
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Ye-Sheng Wei, Yan-Hua Zheng, Wei-Bo Liang, Jian-Zhong Zhang, Zhi-Hui Yang, Mei-Li Lv, Jing Jia, and Lin Zhang
- Subjects
- *
NASOPHARYNX cancer , *PROTEIN analysis , *MASS spectrometry , *CANCER patients , *IONIZATION (Atomic physics) - Abstract
The article focuses on a study which assessed the feasibility of mass spectrometry-based protein profiling for differentiating patients with nasopharyngeal carcinoma (NPC) from noncancer patients. The study analyzed serum samples from patients diagnosed with NPC and from noncancer controls using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). It concluded that SELDI-TOF-MS combined with a tree analysis model can discriminate NPC from noncancer patients.
- Published
- 2008
- Full Text
- View/download PDF
43. The beneficial effect of α-lipoic acid on spinal cord injury repair in rats is mediated through inhibition of oxidative stress: A transcriptomic analysis.
- Author
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Bian MM, Xu YM, Zhang L, Yan HZ, Gao JX, Fu GQ, Wang YY, and Lü HZ
- Abstract
Background: Oxidative stress is a crucial factor contributing to the occurrence and development of secondary damage in spinal cord injuries (SCI), ultimately impacting the recovery process. α-lipoic acid (ALA) exhibits potent antioxidant properties, effectively reducing secondary damage and providing neuroprotective benefits. However, the precise mechanism by which ALA plays its antioxidant role remains unknown., Methods: We established a model of moderate spinal cord contusion in rats. Experimental rats were randomly divided into 3 distinct groups: the sham group, the model control group (SCI_Veh), and the ALA treatment group (SCI_ALA). The sham group rats were exposed only to the SC without contusion injury. Rats belonging to SCI_Veh group were not administered any treatment after SCI. Rats of SCI_ALA group were intraperitoneally injected with the corresponding volume of ALA according to body weight for three consecutive days after the surgery. Subsequently, three days after SCI, spinal cord samples were obtained from three groups of rats: the sham group, model control group, and administration group. Thereafter, total RNA was extracted from the samples and the expression of three sets of differential genes was analyzed by transcriptome sequencing technology. Real-time PCR was used to verify the sequencing results. The impact of ALA on oxidative stress in rats following SCI was assessed by measuring their total antioxidant capacity and hydrogen peroxide (H
2 O2 ) content. The effects of ALA on rat recovery following SCI was investigated through Beattie and Bresnahan (BBB) score and footprint analysis., Results: The findings from the transcriptome sequencing analysis revealed that the model control group had 2975 genes with altered expression levels when compared to the ALA treatment group. Among these genes, 1583 were found to be upregulated while 1392 were down-regulated. Gene ontology (GO) displayed significant enrichment in terms of functionality, specifically in oxidative phosphorylation, oxidoreductase activity, and signaling receptor activity. The Kyoto encyclopedia of genes and genomes (KEGG) pathway was enriched in oxidative phosphorylation, glutathione metabolism and cell cycle. ALA was found to have multiple benefits for rats after SCI, including increasing their antioxidant capacity and reducing H2 O2 levels. Additionally, it was effective in improving motor function (such as 7 days after SCI, the BBB score for SCI_ALA was 8.400 ± 0.937 compared to 7.050 ± 1.141 for SCI_Veh) and promoting histological recovery after SCI (The results of HE demonstrated that the percentage of damage area in was 44.002 ± 6.680 in the SCI_ALA and 57.215 ± 3.964 in the SCI_Veh at the center of injury.). The sequence data from this study has been deposited into Sequence Read Archive (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE242507)., Conclusion: Overall, the findings of this study confirmed the beneficial effects of ALA on recovery in SCI rats through transcriptome sequencing, behavioral, as well histology analyses.- Published
- 2024
- Full Text
- View/download PDF
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