162 results on '"Martin F. Flajnik"'
Search Results
2. Identification of the Fc‐alpha/mu receptor inXenopusprovides insight into the emergence of the poly‐Ig receptor (pIgR) and mucosal Ig transport
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Emily M Flowers, Harold R. Neely, Tereza Almeida, Caitlin D. Castro, Yuko Ohta, Martin F. Flajnik, and Jacqueline Guo
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Fish Proteins ,Immunology ,Receptors, Opioid, mu ,Xenopus ,Immunoglobulins ,Receptors, Fc ,Article ,Xenopus laevis ,Antigens, CD ,Gene duplication ,Animals ,Humans ,Immunology and Allergy ,Receptor ,Immunity, Mucosal ,Gene ,Phylogeny ,biology ,Receptors, Polymeric Immunoglobulin ,biology.organism_classification ,Acquired immune system ,J chain ,Cell biology ,Protein Transport ,biology.protein ,Antibody ,Transcytosis ,Polymeric immunoglobulin receptor - Abstract
The poly-Immunoglobulin Receptor (pIgR) transcytoses J chain-containing antibodies through mucosal epithelia. In mammals, two cis-duplicates of PIGR, FCMR and FCAMR, flank the PIGR gene. A PIGR duplication is first found in amphibians, previously annotated as PIGR2 (herein xlFCAMR), and is expressed by antigen-presenting cells. We demonstrate that xlFcamR is the equivalent of mammalian FcamR. It has been assumed that pIgR is the oldest member of this family, yet our data could not distinguish whether PIGR or FCAMR emerged first; however, FCMR was the last family member to emerge. Interestingly, bony fish 'pIgR' is not an orthologue of tetrapod pIgR, and possibly acquired its function via convergent evolution. PIGR/FCAMR/FCMR are members of a larger superfamily including TREM, CD300, and NKp44, which we name the 'double-disulfide Ig superfamily' (ddIgSF). Domains related to each ddIgSF family were identified in cartilaginous fish (sharks, chimeras) and encoded in a single gene cluster syntenic to the human pIgR locus. Thus, the ddIgSF families date back to earliest antibody-based adaptive immunity, but apparently not before. Finally, our data strongly suggest that the J chain arose in evolution only for Ig multimerization. This study provides a framework for further studies of pIgR and the ddIgSF in vertebrates. This article is protected by copyright. All rights reserved.
- Published
- 2021
3. A Highly Complex, MHC-Linked, 350 Million-Year-Old Shark Nonclassical Class I Lineage
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André M. Machado, Martin F. Flajnik, Fabiana Neves, Tereza Almeida, Arnaud Gaigher, Pedro J. Esteves, Yuko Ohta, Ana Veríssimo, Antonio Muñoz-Mérida, and L. Filipe C. Castro
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Lineage (genetic) ,Immunology ,Vertebrate ,chemical and pharmacologic phenomena ,Biology ,biology.organism_classification ,Major histocompatibility complex ,Chondrichthyes ,Article ,Holocephali ,Evolutionary biology ,biology.animal ,biology.protein ,Immunology and Allergy ,Gene family ,Nurse shark ,Gene - Abstract
Cartilaginous fish, or Chondrichthyes, are the oldest extant vertebrates to possess the MHC and the Ig superfamily–based Ag receptors, the defining genes of the gnathostome adaptive immune system. In this work, we have identified a novel MHC lineage, UEA, a complex multigene nonclassical class I family found in sharks (division Selachii) but not detected in chimaeras (subclass Holocephali) or rays (division Batoidea). This new lineage is distantly related to the previously reported nonclassical class I lineage UCA, which appears to be present only in dogfish sharks (order Squaliformes). UEA lacks conservation of the nine invariant residues in the peptide (ligand)–binding regions (PBR) that bind to the N and C termini of bound peptide in most vertebrate classical class I proteins, which are replaced by relatively hydrophobic residues compared with the classical UAA. In fact, UEA and UCA proteins have the most hydrophobic-predicted PBR of all identified chondrichthyan class I molecules. UEA genes detected in the whale shark and bamboo shark genome projects are MHC linked. Consistent with UEA comprising a very large gene family, we detected weak expression in different tissues of the nurse shark via Northern blotting and RNA sequencing. UEA genes fall into three sublineages with unique characteristics in the PBR. UEA shares structural and genetic features with certain nonclassical class I genes in other vertebrates, such as the highly complex XNC nonclassical class I genes in Xenopus, and we anticipate that each shark gene, or at least each sublineage, will have a unique function, perhaps in bacterial defense.
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- 2021
4. Masanori Kasahara: Long-standing Immunogenetics co-editor steps down
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Martin F. Flajnik
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Immunology ,Genetics ,Immunogenetics - Published
- 2022
5. Lost structural and functional inter-relationships between Ig and TCR loci in mammals revealed in sharks
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Martin F. Flajnik, Yuko Ohta, Michael F. Criscitiello, and Jeannine A. Ott
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0301 basic medicine ,T cell ,Immunology ,Receptors, Antigen, T-Cell ,Immunoglobulins ,Adaptive Immunity ,Article ,Evolution, Molecular ,03 medical and health sciences ,0302 clinical medicine ,Cytidine Deaminase ,biology.animal ,Genetics ,medicine ,Activation-induced (cytidine) deaminase ,Animals ,Humans ,14. Life underwater ,Receptor ,B cell ,Mammals ,biology ,T-cell receptor ,Vertebrate ,Cytidine deaminase ,biology.organism_classification ,Receptors, Antigen ,030104 developmental biology ,medicine.anatomical_structure ,Evolutionary biology ,Sharks ,biology.protein ,Nurse shark ,030215 immunology - Abstract
Immunoglobulins and T cell receptors (TCR) have obvious structural similarities as well as similar immunogenetic diversification and selection mechanisms. Nevertheless, the two receptor systems and the loci that encode them are distinct in humans and classical murine models, and the gene segments comprising each repertoire are mutually exclusive. Additionally, while both B and T cells employ recombination-activating genes (RAG) for primary diversification, immunoglobulins are afforded a supplementary set of activation-induced cytidine deaminase (AID)-mediated diversification tools. As the oldest-emerging vertebrates sharing the same adaptive B and T cell receptor systems as humans, extant cartilaginous fishes allow a potential view of the ancestral immune system. In this review, we discuss breakthroughs we have made in studies of nurse shark (Ginglymostoma cirratum) T cell receptors demonstrating substantial integration of loci and diversification mechanisms in primordial B and T cell repertoires. We survey these findings in this shark model where they were first described, while noting corroborating examples in other vertebrate groups. We also consider other examples where the gnathostome common ancestry of the B and T cell receptor systems have allowed dovetailing of genomic elements and AID-based diversification approaches for the TCR. The cartilaginous fish seem to have retained this T/B cell plasticity to a greater extent than more derived vertebrate groups, but representatives in all vertebrate taxa except bony fish and placental mammals show such plasticity.
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- 2021
6. Nurse shark T‐cell receptors employ somatic hypermutation preferentially to alter alpha/delta variable segments associated with alpha constant region
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Jeannine A. Ott, Jenna Harrison, Michael F. Criscitiello, and Martin F. Flajnik
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0301 basic medicine ,Gene Rearrangement, delta-Chain T-Cell Antigen Receptor ,Immunology ,Somatic hypermutation ,chemical and pharmacologic phenomena ,medicine.disease_cause ,Article ,Affinity maturation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cytidine Deaminase ,medicine ,Animals ,Immunology and Allergy ,Mutation ,biology ,T-cell receptor ,breakpoint cluster region ,hemic and immune systems ,Cytidine ,biology.organism_classification ,Delta-v (physics) ,Cell biology ,030104 developmental biology ,chemistry ,Sharks ,Somatic Hypermutation, Immunoglobulin ,Nurse shark ,Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor ,Immunoglobulin Heavy Chains ,030215 immunology - Abstract
In addition to canonical TCR and BCR, cartilaginous fish assemble noncanonical TCR that employ various B-cell components. For example, shark T cells associate alpha (TCR-α) or delta (TCR-δ) constant (C) regions with Ig heavy chain (H) variable (V) segments or TCR-associated Ig-like V (TAILV) segments to form chimeric IgV-TCR, and combine TCRδC with both Ig-like and TCR-like V segments to form the doubly rearranging NAR-TCR. Activation-induced (cytidine) deaminase-catalyzed somatic hypermutation (SHM), typically used for B-cell affinity maturation, also is used by TCR-α during selection in the shark thymus presumably to salvage failing receptors. Here, we found that the use of SHM by nurse shark TCR varies depending on the particular V segment or C region used. First, SHM significantly alters alpha/delta V (TCRαδV) segments using TCR αC but not δC. Second, mutation to IgHV segments associated with TCR δC was reduced compared to mutation to TCR αδV associated with TCR αC. Mutation was present but limited in V segments of all other TCR chains including NAR-TCR. Unexpectedly, we found preferential rearrangement of the noncanonical IgHV-TCRδC over canonical TCR αδV-TCRδC receptors. The differential use of SHM may reveal how activation-induced (cytidine) deaminase targets V regions.
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- 2020
7. Ancient Use of Ig Variable Domains Contributes Significantly to the TCRδ Repertoire
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Martin F. Flajnik, Michael F. Criscitiello, Thaddeus C. Deiss, Caitlin D. Castro, Rebecca A Daniel, Jeannine A. Ott, Yuko Ohta, Patricia L. Chen, and Breanna Breaux
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Gene Rearrangement, delta-Chain T-Cell Antigen Receptor ,Immunology ,Immunoglobulin Variable Region ,chemical and pharmacologic phenomena ,Locus (genetics) ,Article ,03 medical and health sciences ,0302 clinical medicine ,Phylogenetics ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Phylogeny ,biology ,Repertoire ,T-cell receptor ,breakpoint cluster region ,Chromosome ,Receptors, Antigen, T-Cell, gamma-delta ,Gene rearrangement ,biology.organism_classification ,Evolutionary biology ,Sharks ,Immunoglobulin Domains ,Nurse shark ,Sequence Alignment ,030215 immunology - Abstract
The loci encoding B and T cell Ag receptors are generally distinct in commonly studied mammals, with each receptor’s gene segments limited to intralocus, cis chromosomal rearrangements. The nurse shark (Ginglymostoma cirratum) represents the oldest vertebrate class, the cartilaginous fish, with adaptive immunity provided via Ig and TCR lineages, and is one species among a growing number of taxa employing Ig-TCRδ rearrangements that blend these distinct lineages. Analysis of the nurse shark Ig-TCRδ repertoire found that these rearrangements possess CDR3 characteristics highly similar to canonical TCRδ rearrangements. Furthermore, the Ig-TCRδ rearrangements are expressed with TCRγ, canonically found in the TCRδ heterodimer. We also quantified BCR and TCR transcripts in the thymus for BCR (IgHV-IgHC), chimeric (IgHV-TCRδC), and canonical (TCRδV-TCRδC) transcripts, finding equivalent expression levels in both thymus and spleen. We also characterized the nurse shark TCRαδ locus with a targeted bacterial artifical chromosome sequencing approach and found that the TCRδ locus houses a complex of V segments from multiple lineages. An IgH-like V segment, nestled within the nurse shark TCRδ translocus, grouped with IgHV-like rearrangements we found expressed with TCRδ (but not IgH) rearrangements in our phylogenetic analysis. This distinct lineage of TCRδ-associated IgH-like V segments was termed “TAILVs.” Our data illustrate a dynamic TCRδ repertoire employing TCRδVs, NARTCRVs, bona fide trans-rearrangements from shark IgH clusters, and a novel lineage in the TCRδ-associated Ig-like V segments.
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- 2019
8. Origin and evolution of the specialized forms of proteasomes involved in antigen presentation
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Masanori Kasahara and Martin F. Flajnik
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0301 basic medicine ,Proteasome Endopeptidase Complex ,T-Lymphocytes ,Protein subunit ,Immunology ,Antigen presentation ,Receptors, Antigen, T-Cell ,Adaptive Immunity ,Major histocompatibility complex ,Article ,Evolution, Molecular ,03 medical and health sciences ,0302 clinical medicine ,Gene duplication ,Genetics ,Animals ,Humans ,Gene ,Antigen Presentation ,biology ,Histocompatibility Antigens Class I ,T-cell receptor ,Acquired immune system ,030104 developmental biology ,Proteasome ,biology.protein ,030215 immunology - Abstract
Proteasomes are a multi-subunit protease complex that produces peptides bound by major histocompatibility complex (MHC) class I molecules. Phylogenetic studies indicate that two specialized forms of proteasomes, immunoproteasomes and thymoproteasomes, and the proteasome activator PA28αβ emerged in a common ancestor of jawed vertebrates which acquired adaptive immunity based on the MHC, T cell receptors, and B cell receptors ~ 500 million years ago. Comparative genomics studies now provide strong evidence that the genes coding for the immunoproteasome subunits emerged by genome-wide duplication. On the other hand, the gene encoding the thymoproteasome subunit β5t emerged by tandem duplication from the gene coding for the β5 subunit. Strikingly, birds lack immunoproteasomes, thymoproteasomes, and the proteasome activator PA28αβ, raising an interesting question of whether they have evolved any compensatory mechanisms.
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- 2019
9. Analysis of shark NCR3 family genes reveals primordial features of vertebrate NKp30
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Jianxin Sui, Tereza Almeida, Morgan E Janes, Jacob Kincer, Yuko Ohta, Hanover Matz, Allison Kinlein, Michael F. Criscitiello, and Martin F. Flajnik
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0301 basic medicine ,Immunology ,Sequence Homology ,chemical and pharmacologic phenomena ,In situ hybridization ,Major histocompatibility complex ,Article ,Major Histocompatibility Complex ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Animals ,Amino Acid Sequence ,Receptor ,Gene ,B cell ,Phylogeny ,Innate immune system ,Natural Cytotoxicity Triggering Receptor 3 ,biology ,Acquired immune system ,Cell biology ,Killer Cells, Natural ,030104 developmental biology ,medicine.anatomical_structure ,biology.protein ,Sharks ,Immunoglobulin superfamily ,030215 immunology - Abstract
Natural killer (NK) cells play major roles in innate immunity against viruses and cancer. Natural killer receptors (NKR) expressed by NK cells recognize foreign- or self-ligands on infected and transformed cells as well as healthy cells. NKR genes are the most rapidly evolving loci in vertebrates, and it is generally difficult to detect orthologues in different taxa. The unique exception is NKp30, an activating NKR in mammals that binds to the self-ligand B7H6. The NKp30-encoding gene, NCR3, has been found in most vertebrates including sharks, the oldest vertebrates with human-type adaptive immunity. NCR3 has a special, non-rearranging VJ-type immunoglobulin superfamily (IgSF) domain that predates the emergence of the rearranging antigen receptors. Herein we show that NCR3 loci are linked to the shark major histocompatibility complex (MHC), proving NCR3's primordial association with the MHC. We identified eight subtypes of differentially expressed highly divergent shark NCR3 family genes. Using in situ hybridization, we detected one subtype, NS344823, to be expressed by predominantly single cells outside of splenic B cell zones. The expression by non-B cells was also confirmed by PCR in peripheral blood lymphocytes. Surprisingly, high expression of NS344823 was detected in the thymic cortex, demonstrating NS344823 expression in developing T cells. Finally, we show for the first time that shark T cells are found as single cells or in small clusters in the splenic red pulp, also unassociated with the large B cell follicles we previously identified.
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- 2020
10. Cartilaginous fish class II genes reveal unprecedented old allelic lineages and confirm the late evolutionary emergence of DM
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Antonio Muñoz-Mérida, Martin F. Flajnik, Ana Veríssimo, Pedro J. Esteves, L. Filipe C. Castro, Fabiana Neves, Tereza Almeida, Arnaud Gaigher, and Yuko Ohta
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0301 basic medicine ,Lineage (evolution) ,Immunology ,Genes, MHC Class II ,Adaptive immunity ,Biology ,Major histocompatibility complex ,Genome ,Article ,MHC Class II Gene ,03 medical and health sciences ,0302 clinical medicine ,Basal jawed vertebrates ,biology.animal ,Animals ,Amino Acid Sequence ,Skates, Fish ,Antigen presentation ,Molecular Biology ,Gene ,Alleles ,Phylogeny ,MHC class II ,Phylogenetic tree ,Histocompatibility Antigens Class II ,Vertebrate ,030104 developmental biology ,Evolutionary biology ,Sharks and rays ,biology.protein ,Sharks ,MHC ,030215 immunology - Abstract
Cartilaginous fish (chimaeras, rays and sharks) are the most basal extant jawed vertebrates with an adaptive immune system based on the Major Histocompatibility Complex (MHC). Despite being a key taxon in the evolution of vertebrate adaptive immunity, no comprehensive characterization of MHC class II genes has been undertaken for the group. We performed extensive bioinformatic searches on a taxonomically diverse dataset of transcriptomes and genomes of cartilaginous fish targeting MHC class II sequences. Class IIα and IIβ sequences were retrieved from all taxa analyzed and showed typical features of classical class II genes. Phylogenetic trees of the immunoglobulin superfamily domain showed two divergent and remarkably ancient lineages of class II genes in Selachians (sharks), originating >350 million years ago. Close linkage of lineage-specific pairs of IIα and IIβ genes was found, confirming previous results, with genes from distinct lineages segregating as alleles. Nonclassical class II DM sequences were not retrieved from these data and classical class II sequences lacked the conserved residues shown to interact with DM molecules, supporting claims that the DM system arose only in the lobe-finned fish lineage leading to tetrapods. Based on our search methods, other divergent class II genes are unlikely in cartilaginous fish.
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- 2020
11. Decision letter: The structures of secretory and dimeric immunoglobulin A
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Martin F. Flajnik and Sjors H.W. Scheres
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Immunoglobulin A ,Biochemistry ,biology ,Chemistry ,biology.protein - Published
- 2020
12. Author response for 'Nurse Shark T Cell Receptors Employ Somatic Hypermutation Preferentially to Alter Alpha/Delta Variable Segments Associated with Alpha Constant Region'
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Martin F. Flajnik, Jenna Harrison, Michael F. Criscitiello, and Jeannine A. Ott
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Delta ,Constant region ,T-cell receptor ,Somatic hypermutation ,Alpha (ethology) ,Biology ,Nurse shark ,biology.organism_classification ,Cell biology - Published
- 2020
13. Haptoglobin Is a Divergent MASP Family Member That Neofunctionalized To Recycle Hemoglobin via CD163 in Mammals
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Helen Dooley, Yuko Ohta, Anthony K. Redmond, Michael F. Criscitiello, Martin F. Flajnik, and Daniel J. Macqueen
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Fish Proteins ,0301 basic medicine ,Immunology ,Antigens, Differentiation, Myelomonocytic ,Receptors, Cell Surface ,Plasma protein binding ,Hemolysis ,Article ,Hemoglobins ,03 medical and health sciences ,Species Specificity ,Antigens, CD ,Phylogenetics ,biology.animal ,Animals ,Humans ,Immunology and Allergy ,Cloning, Molecular ,Receptor ,Phylogeny ,Mammals ,Genome ,Haptoglobins ,biology ,Haptoglobin ,Vertebrate ,biology.organism_classification ,Biological Evolution ,Cell biology ,030104 developmental biology ,Mannose-Binding Protein-Associated Serine Proteases ,Oncorhynchus mykiss ,Sharks ,biology.protein ,Hemoglobin ,Nurse shark ,CD163 ,Acute-Phase Proteins ,Protein Binding - Abstract
In mammals, haptoglobin (Hp) is an acute-phase plasma protein that binds with high affinity to hemoglobin (Hb) released by intravascular hemolysis. The resultant Hp–Hb complexes are bound and cleared by the scavenger receptor CD163, limiting Hb-induced oxidative damage. In this study, we show that Hp is a divergent member of the complement-initiating MASP family of proteins, which emerged in the ancestor of jawed vertebrates. We demonstrate that Hp has been independently lost from multiple vertebrate lineages, that characterized Hb-interacting residues of mammals are poorly conserved in nonmammalian species maintaining Hp, and that the extended loop 3 region of Hp, which mediates CD163 binding, is present only in mammals. We show that the Hb-binding ability of cartilaginous fish (nurse shark, Ginglymostoma cirratum; small-spotted catshark, Scyliorhinus canicula; and thornback ray, Raja clavata) and teleost fish (rainbow trout, Oncorhynchus mykiss) Hp is species specific, and where binding does occur it is likely mediated through a different structural mechanism to mammalian Hp. The continued, high-level expression of Hp in cartilaginous fishes in which Hb binding is not evident signals that Hp has (an)other, yet unstudied, role(s) in these species. Previous work indicates that mammalian Hp also has secondary, immunomodulatory functions that are independent of Hb binding; our work suggests these may be remnants of evolutionary more ancient functions, retained after Hb removal became the primary role of Hp in mammals.
- Published
- 2018
14. A cold-blooded view of adaptive immunity
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Martin F. Flajnik
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0301 basic medicine ,History ,Lymphoid Tissue ,Lymphocyte ,Adaptive Immunity ,Biology ,Major histocompatibility complex ,Article ,Education ,Evolution, Molecular ,Major Histocompatibility Complex ,03 medical and health sciences ,Immunity ,medicine ,Animals ,Humans ,Lymphocytes ,Receptor ,Immunity, Mucosal ,Fishes ,Genetic Variation ,Acquired immune system ,Bony fish ,Immunity, Innate ,Computer Science Applications ,Immunoglobulin Isotypes ,Receptors, Antigen ,030104 developmental biology ,medicine.anatomical_structure ,Evolutionary biology ,Vertebrates ,biology.protein ,%22">Fish ,Antigen receptors - Abstract
The adaptive immune system arose 500 million years ago in ectothermic (cold-blooded) vertebrates. Classically, the adaptive immune system has been defined by the presence of lymphocytes expressing recombination-activating gene (RAG)-dependent antigen receptors and the MHC. These features are found in all jawed vertebrates, including cartilaginous and bony fish, amphibians and reptiles and are most likely also found in the oldest class of jawed vertebrates, the extinct placoderms. However, with the discovery of an adaptive immune system in jawless fish based on an entirely different set of antigen receptors — the variable lymphocyte receptors — the divergence of T and B cells, and perhaps innate-like lymphocytes, goes back to the origin of all vertebrates. This Review explores how recent developments in comparative immunology have furthered our understanding of the origins and function of the adaptive immune system.
- Published
- 2018
15. 'Double-duty' conventional dendritic cells in the amphibianXenopusas the prototype for antigen presentation to B cells
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Jacqueline Guo, Harold R. Neely, Martin F. Flajnik, Emily M Flowers, and Michael F. Criscitiello
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0301 basic medicine ,T-Lymphocytes ,Immunology ,Antigen presentation ,Somatic hypermutation ,Article ,Affinity maturation ,Xenopus laevis ,03 medical and health sciences ,0302 clinical medicine ,Species Specificity ,Animals ,Immunology and Allergy ,Mammals ,Antigen Presentation ,B-Lymphocytes ,MHC class II ,biology ,Follicular dendritic cells ,Germinal center ,Dendritic Cells ,Dendritic cell ,Germinal Center ,Immunoglobulin Class Switching ,Cell biology ,030104 developmental biology ,Immunoglobulin class switching ,biology.protein ,Somatic Hypermutation, Immunoglobulin ,Dendritic Cells, Follicular ,Spleen ,030215 immunology - Abstract
Two populations of dendritic cells (DCs) are found in mammals, one derived from hematopoietic precursors (conventional/cDC), and another derived from mesenchymal precursors, the follicular DC (FDC); the latter is specialized for antigen presentation to B cells, and has only been definitively demonstrated in mammals. Both cDC and FDC are necessary for induction of germinal centers (GC) and GC-dependent class switch recombination (CSR) and somatic hypermutation (SHM). We demonstrate that in Xenopus, an amphibian in which immunoglobulin CSR and SHM occur without GC formation, a single type of DC has properties of both cDC and FDC, including high expression of MHC class II for the former and display of native antigen at the cell surface for the latter. Our data confirm that the advent of FDC functionality preceded emergence of bona fide FDC, which was in turn crucial for the development of GC formation and efficient affinity maturation in mammals.
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- 2018
16. Evidence for Ig Light Chain Isotype Exclusion in Shark B Lymphocytes Suggests Ordered Mechanisms
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Anita Lui, Anna Iacoangeli, Martin F. Flajnik, Ashley Haines, Ellen Hsu, and Yuko Ohta
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Fish Proteins ,Male ,0301 basic medicine ,Genetic Structures ,Immunology ,Population ,B-Lymphocyte Subsets ,Receptors, Antigen, B-Cell ,Biology ,Immunoglobulin light chain ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Gene Rearrangement, B-Lymphocyte, Light Chain ,Immunology and Allergy ,RNA, Messenger ,education ,Cells, Cultured ,B cell ,B-Lymphocytes ,education.field_of_study ,breakpoint cluster region ,Receptor editing ,Gene rearrangement ,Immunoglobulin Class Switching ,Molecular biology ,Isotype ,Immunoglobulin Isotypes ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Immunoglobulin class switching ,Genetic Loci ,Vertebrates ,Molecular and Structural Immunology ,Sharks ,Female ,Immunoglobulin Light Chains ,030215 immunology - Abstract
Unlike most vertebrates, the shark IgL gene organization precludes secondary rearrangements that delete self-reactive VJ rearranged genes. Nurse sharks express four L chain isotypes, κ, λ, σ, and σ-2, encoded by 35 functional minigenes or clusters. The sequence of gene activation/expression and receptor editing of these isotypes have not been studied. We therefore investigated the extent of isotypic exclusion in separated B cell subpopulations. Surface Ig (sIg)κ–expressing cells, isolated with mAb LK14 that recognizes Cκ, carry predominantly nonproductive rearrangements of other L chain isotypes. Conversely, after depletion with LK14, sIgM+ cells contained largely nonproductive κ and enrichment for in-frame VJ of the others. Because some isotypic inclusion was observed at the mRNA level, expression in the BCR was examined. Functional λ mRNA was obtained, as expected, from the LK14-depleted population, but was also in sIgκ+ splenocytes. Whereas λ somatic mutants from the depleted sample displayed evidence of positive selection, the λ genes in sIgκ+ cells accumulated bystander mutations indicating a failure to express their products at the cell surface in association with the BCR H chain. In conclusion, a shark B cell expresses one L chain isotype at the surface and other isotypes as nonproductive VJ, sterile transcripts, or in-frame VJ whose products may not associate with the H chain. Based on the mRNA content found in the B cell subpopulations, an order of L chain gene activation is suggested as: σ-2 followed by κ, then σ and λ.
- Published
- 2017
17. An Ancient, MHC-Linked, Nonclassical Class I Lineage in Cartilaginous Fish
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Pedro J. Esteves, Ana Veríssimo, Tereza Almeida, Yuko Ohta, and Martin F. Flajnik
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Lineage (genetic) ,Polymorphism, Genetic ,biology ,Immunology ,Gene Dosage ,Genes, MHC Class I ,Adaptive Immunity ,biology.organism_classification ,Major histocompatibility complex ,Article ,Holocephali ,Elasmobranchii ,Evolutionary biology ,MHC class I ,biology.protein ,Sharks ,Immunology and Allergy ,Animals ,Amino Acid Sequence ,Nurse shark ,Low copy number ,Gene ,Conserved Sequence ,Phylogeny - Abstract
Cartilaginous fishes, or chondrichthyans, are the oldest jawed vertebrates that have an adaptive immune system based on the MHC and Ig superfamily–based AgR. In this basal group of jawed vertebrates, we identified a third nonclassical MHC class I lineage (UDA), which is present in all species analyzed within the two major cartilaginous subclasses, Holocephali (chimaeras) and Elasmobranchii (sharks, skates, and rays). The deduced amino acid sequences of UDA have eight out of nine typically invariant residues that bind to the N and C termini of bound peptide found in most vertebrae classical class I (UAA); additionally, the other predicted 28 peptide-binding residues are perfectly conserved in all elasmobranch UDA sequences. UDA is distinct from UAA in its differential tissue distribution and its lower expression levels and is mono- or oligomorphic unlike the highly polymorphic UAA. UDA has a low copy number in elasmobranchs but is multicopy in the holocephalan spotted ratfish (Hydrolagus colliei). Using a nurse shark (Ginglymostoma cirratum) family, we found that UDA is MHC linked but separable by recombination from the tightly linked cluster of UAA, TAP, and LMP genes, the so-called class I region found in most nonmammalian vertebrates. UDA has predicted structural features that are similar to certain nonclassical class I genes in other vertebrates, and, unlike polymorpic classical class I, we anticipate that it may bind to a conserved set of specialized peptides.
- Published
- 2019
18. Inferring the 'Primordial Immune Complex': Origins of MHC Class I and Antigen Receptors Revealed by Comparative Genomics
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Masanori Kasahara, Martin F. Flajnik, Timothy D O'Connor, and Yuko Ohta
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Genetics ,Comparative genomics ,biology ,Immunology ,Histocompatibility Antigens Class I ,Xenopus ,CD1 ,chemical and pharmacologic phenomena ,biochemical phenomena, metabolism, and nutrition ,Xenopus Proteins ,biology.organism_classification ,Major histocompatibility complex ,Article ,Antigens, CD1 ,Exon ,Xenopus laevis ,Antigen ,MHC class I ,Databases, Genetic ,biology.protein ,Immunology and Allergy ,Animals ,Gene - Abstract
Comparative analyses suggest that the MHC was derived from a prevertebrate “primordial immune complex” (PIC). PIC duplicated twice in the well-studied two rounds of genome-wide duplications (2R) early in vertebrate evolution, generating four MHC paralogous regions (predominantly on human chromosomes [chr] 1, 6, 9, 19). Examining chiefly the amphibian Xenopus laevis, but also other vertebrates, we identified their MHC paralogues and mapped MHC class I, AgR, and “framework” genes. Most class I genes mapped to MHC paralogues, but a cluster of Xenopus MHC class Ib genes (xnc), which previously was mapped outside of the MHC paralogues, was surrounded by genes syntenic to mammalian CD1 genes, a region previously proposed as an MHC paralogue on human chr 1. Thus, this gene block is instead the result of a translocation that we call the translocated part of the MHC paralogous region (MHCtrans). Analyses of Xenopus class I genes, as well as MHCtrans, suggest that class I arose at 1R on the chr 6/19 ancestor. Of great interest are nonrearranging AgR-like genes mapping to three MHC paralogues; thus, PIC clearly contained several AgR precursor loci, predating MHC class I/II. However, all rearranging AgR genes were found on paralogues derived from the chr 19 precursor, suggesting that invasion of a variable (V) exon by the RAG transposon occurred after 2R. We propose models for the evolutionary history of MHC/TCR/Ig and speculate on the dichotomy between the jawless (lamprey and hagfish) and jawed vertebrate adaptive immune systems, as we found genes related to variable lymphocyte receptors also map to MHC paralogues.
- Published
- 2019
19. Author Correction: Elephant shark genome provides unique insights into gnathostome evolution
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Manuel Irimia, Byrappa Venkatesh, Sydney Brenner, Masanori Kasahara, Jeremy B. Swann, Yoichi Sutoh, Yuko Ohta, Wesley C. Warren, Brian J. Raney, Shawn Hoon, Richard K. Wilson, Vydianathan Ravi, Shufen Ho, Patrick Minx, LaDeana W. Hillier, Sumanty Tohari, Vamshidhar Gangu, Belen Lorente-Galdos, Alice Tay, Michelle M. Lian, Ashish K. Maurya, Scott William Roy, Igor Kondrychyn, Philip W. Ingham, Alison P. Lee, Zhi Wei Lim, Martin F. Flajnik, Vladimir Korzh, Kiat Whye Kong, Javier Quilez, Thomas Boehm, Boon-Hui Tay, and Tomas Marques-Bonet
- Subjects
Fish Proteins ,Time Factors ,T-Lymphocytes ,Molecular Sequence Data ,MEDLINE ,Computational biology ,Biology ,Genome ,Evolution, Molecular ,Osteogenesis ,Animals ,Cell Lineage ,Author Correction ,Phylogeny ,Zebrafish ,Immunity, Cellular ,Multidisciplinary ,Published Erratum ,Molecular Sequence Annotation ,Genomics ,Phosphoproteins ,Protein Structure, Tertiary ,Vertebrates ,Sharks ,Calcium ,Gene Deletion - Abstract
The emergence of jawed vertebrates (gnathostomes) from jawless vertebrates was accompanied by major morphological and physiological innovations, such as hinged jaws, paired fins and immunoglobulin-based adaptive immunity. Gnathostomes subsequently diverged into two groups, the cartilaginous fishes and the bony vertebrates. Here we report the whole-genome analysis of a cartilaginous fish, the elephant shark (Callorhinchus milii). We find that the C. milii genome is the slowest evolving of all known vertebrates, including the 'living fossil' coelacanth, and features extensive synteny conservation with tetrapod genomes, making it a good model for comparative analyses of gnathostome genomes. Our functional studies suggest that the lack of genes encoding secreted calcium-binding phosphoproteins in cartilaginous fishes explains the absence of bone in their endoskeleton. Furthermore, the adaptive immune system of cartilaginous fishes is unusual: it lacks the canonical CD4 co-receptor and most transcription factors, cytokines and cytokine receptors related to the CD4 lineage, despite the presence of polymorphic major histocompatibility complex class II molecules. It thus presents a new model for understanding the origin of adaptive immunity.
- Published
- 2020
20. Questions of Stochasticity and Control in Immune Repertoires
- Author
-
Martin F. Flajnik, Gur Yaari, and Uri Hershberg
- Subjects
0301 basic medicine ,Immunology ,Receptors, Antigen, B-Cell ,Autoimmunity ,Biology ,Adaptive Immunity ,Epigenesis, Genetic ,Evolution, Molecular ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Immunology and Allergy ,Animals ,Humans ,Control (linguistics) ,Clonal Selection, Antigen-Mediated ,B-Lymphocytes ,Stochastic Processes ,Models, Immunological ,Biological evolution ,Acquired immune system ,Biological Evolution ,Clone Cells ,030104 developmental biology ,Evolutionary biology ,Somatic Hypermutation, Immunoglobulin ,030215 immunology ,Clonal selection - Published
- 2018
21. Author response: Somatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymus
- Author
-
Martin F. Flajnik, Jeannine A. Ott, Yuko Ohta, Thaddeus C. Deiss, Michael F. Criscitiello, and Caitlin D. Castro
- Subjects
biology ,T-cell receptor ,Somatic hypermutation ,Nurse shark ,biology.organism_classification ,Selection (genetic algorithm) ,Cell biology - Published
- 2017
22. Putting J Chain Back on the Map: How Might Its Expression Define Plasma Cell Development?
- Author
-
Martin F. Flajnik and Caitlin D. Castro
- Subjects
Immunoglobulin A ,Plasma Cells ,Immunology ,Biology ,Plasma cell ,Article ,Mice ,medicine ,Animals ,Humans ,Immunology and Allergy ,B cell ,Regulation of gene expression ,Receptors, Polymeric Immunoglobulin ,Phenotype ,Molecular biology ,J chain ,Protein Transport ,medicine.anatomical_structure ,Gene Expression Regulation ,Immunoglobulin M ,Immunoglobulin J-Chains ,biology.protein ,Immunoglobulin J Chain - Abstract
Joining chain (J chain) is a small polypeptide that regulates multimerization of secretory IgM and IgA, the only two mammalian Igs capable of forming multimers. J chain also is required for poly-Ig receptor–mediated transport of these Ig classes across the mucosal epithelium. It is generally assumed that all plasma cells express J chain regardless of expressed isotype, despite the documented presence of J chain− plasma cells in mammals, specifically in all monomeric IgA-secreting cells and some IgG-secreting cells. Compared with most other immune molecules, J chain has not been studied extensively, in part because of technical limitations. Even the reported phenotype of the J chain–knockout mouse is often misunderstood or underappreciated. In this short review, we discuss J chain in light of the various proposed models of its expression and regulation, with an added focus on its evolutionary significance, as well as its expression in different B cell lineages/differentiation states.
- Published
- 2014
23. Somatic hypermutation of T cell receptor gamma, delta, and beta chains in nurse sharks
- Author
-
Jeannine A Ott, Jenna Harrison, Yuko Ota, Martin F Flajnik, and Michael F Criscitiello
- Subjects
Immunology ,Immunology and Allergy - Abstract
Many γδ T cells typically bind antigen in a manner similar to that of immunoglobulins, recognizing and directly binding small molecules and intact proteins without presentation by classical MHC:Ag complexes. In this way, γδ T cells combine an innate-like immune response with an adaptive recognition strategy, providing both an immediate response to pathogen invasion and an ongoing, adaptive response to inflammation. However, γδ T cells often recombine tissue-specific, restricted sets of genes that have limited junctional diversity. Thus, somatic hypermutation (SHM) presents a useful solution for diversifying these antibody-like T cell receptors (TCR) by fine-tuning ligand recognition or permitting changes within the loci that allow receptors to evolve more rapidly to changing ligand environments. In fact, recent studies in sandbar shark have confirmed definitively that the γ chain locus of γδ T cells employs SHM as a mechanism to generate more diverse γδ TCR, and follow-up studies in dromedary camel indicated that loci of both γ and δ chains use SHM to diversify γδ TCR. In nurse sharks, we found evidence that SHM acts in the thymus, altering the α chain locus of αβ T cells to broaden the primary αβ T cell repertoire. Further, early results suggest that SHM modifies both γ and δ chain loci (but not β chain locus of αβ T cells) and this alteration may occur in the thymus, indicating that this receptor fine-tuning may precede ligand recognition.
- Published
- 2019
24. Noncoordinate expression of J-chain and Blimp-1 define nurse shark plasma cell populations during ontogeny
- Author
-
Helen Dooley, Caitlin D. Castro, Martin F. Flajnik, and Yuko Ohta
- Subjects
Cellular differentiation ,Plasma Cells ,Immunology ,Plasma cell ,Article ,medicine ,Animals ,Immunology and Allergy ,biology ,PAX5 Transcription Factor ,Cell Differentiation ,Acquired immune system ,biology.organism_classification ,Molecular biology ,J chain ,Up-Regulation ,Repressor Proteins ,B-1 cell ,medicine.anatomical_structure ,Immunoglobulin M ,Immunoglobulin J-Chains ,Sharks ,biology.protein ,Antibody ,Nurse shark ,Spleen - Abstract
B-lymphocyte-induced maturation protein 1 (Blimp-1) is the master regulator of plasma cell development, controlling genes such as those encoding J-chain and secretory Ig heavy chain. However, some mammalian plasma cells do not express J-chain, and mammalian B1 cells secrete "natural" IgM antibodies without upregulating Blimp-1. While these results have been controversial in mammalian systems, here we describe subsets of normally occurring Blimp-1(-) antibody-secreting cells in nurse sharks, found in lymphoid tissues at all ontogenic stages. Sharks naturally produce large amounts of both pentameric (classically "19S") and monomeric (classically "7S") IgM, the latter an indicator of adaptive immunity. Consistent with the mammalian paradigm, shark Blimp-1 is expressed in splenic 7S IgM-secreting cells, though rarely detected in the J-chain(+) cells producing 19S IgM. Although IgM transcript levels are lower in J-chain(+) cells, these cells nevertheless secrete 19S IgM in the absence of Blimp-1, as demonstrated by ELISPOT and metabolic labeling. Additionally, cells in the shark BM equivalent (epigonal) are Blimp-1(-). Our data suggest that, in sharks, 19S-secreting cells and other secreting memory B cells in the epigonal are maintained for long periods without Blimp-1, but like in mammals, Blimp-1 is required for terminating the B-cell program following an adaptive immune response in the spleen.
- Published
- 2013
25. Editorial: Infection and immunity research at the University of Maryland, Baltimore
- Author
-
James B. Kaper, Harry L. T. Mobley, and Martin F. Flajnik
- Subjects
0301 basic medicine ,Microbiology (medical) ,Gerontology ,Microbial pathogenesis ,Infectious Disease Medicine ,General Immunology and Microbiology ,Universities ,business.industry ,Library science ,General Medicine ,Translational Research, Biomedical ,03 medical and health sciences ,030104 developmental biology ,Infectious Diseases ,Infectious disease (medical specialty) ,Allergy and Immunology ,Baltimore ,Immunology and Allergy ,Medicine ,Humans ,business - Abstract
This special issue of Pathogens and Disease focuses on the research in understanding, treating and preventing a variety of infectious diseases that is being conducted at the University of Maryland, Baltimore (UMB). This university features three internationally renowned research institutes founded by three of the most highly cited investigators in the area of microbiology and the immunology of infectious diseases: The Center for Vaccine Development (CVD) (Myron M. Levine, Founder), The Institute of Human Virology (Robert C. Gallo, Founder) and the Institute for Genome Sciences (Claire M. Fraser, Founder). Two academic departments (Microbiology and Immunology in the UM School of Medicine and Microbial Pathogenesis in the UM School of Dentistry) house additional researchers in this area. UMB is located in the Baltimore–Washington corridor, which is intellectually rich with numerous other institutions conducting infectious disease research including the National Institutes of Health, the Johns Hopkins University, Uniformed Services University, the Walter Reed Army Institute of Research, the US Army Medical Research Institute of Infectious Diseases and the … [↵][1]* Corresponding author: Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 W. Baltimore St. HSF I-Rm 380, Baltimore, MD 21201, USA. Tel: +410-706-2344; Fax: +410-706-6970; E-mail: jkaper{at}medicine.umaryland.edu [1]: #xref-corresp-1-1
- Published
- 2016
26. Genome evolution in the allotetraploid frog Xenopus laevis
- Author
-
Shuji Takahashi, Yutaka Suzuki, Douglas W. Houston, Christian D. Haudenschild, Tsutomu Kinoshita, Darwin S. Dichmann, Shuuji Mawaribuchi, Masanori Taira, Jane Grimwood, Martin F. Flajnik, Yumi Izutsu, Tatsuo Michiue, Michihiko Ito, Yoko Kuroki, Yuzuru Ito, Yuko Ohta, Oleg Simakov, Ila van Kruijsbergen, Taejoon Kwon, Shengquiang Shu, Jacob O. Kitzman, Edward M. Marcotte, Adam M. Session, Yuuri Yasuoka, Sahar V. Mozaffari, Jonathan C. Stites, Jay Shendure, Minoru Watanabe, Joseph W. Carlson, Rebecca Heald, Nicholas H. Putnam, Akimasa Fukui, John B. Wallingford, Aaron M. Zorn, Kevin A. Burns, Atsushi Suzuki, Sven Heinz, Jarrod Chapman, Therese Mitros, Hajime Ogino, Georgios Georgiou, Makoto Asashima, Kamran Karimi, Uffe Hellsten, Jeremy Schmutz, Daniel S. Rokhsar, Joshua D. Fortriede, Yoshinobu Uno, Vaneet Lotay, Jerry Jenkins, Simon J. van Heeringen, Akira Hikosaka, Toshiaki Tanaka, Atsushi Toyoda, Yoshikazu Haramoto, Sarita S. Paranjpe, Chiyo Takagi, Yoichi Matsuda, Takuya Nakayama, Takamasa S. Yamamoto, Ryan Lister, Asao Fujiyama, Richard M. Harland, Ian K. Quigley, Kelly E. Miller, Louis DuPasquier, Peter D. Vize, Gert Jan C. Veenstra, Mariko Kondo, Ozren Bogdanovic, Haruki Ochi, Jessica B. Lyons, Jacques Robert, and Naoto Ueno
- Subjects
0301 basic medicine ,Transposable element ,Genome evolution ,Evolution ,General Science & Technology ,Pseudogene ,Xenopus ,Karyotype ,Biology ,Genome ,Chromosomes ,Evolution, Molecular ,03 medical and health sciences ,Xenopus laevis ,0302 clinical medicine ,Molecular evolution ,Genetics ,Animals ,Molecular Biology ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) ,Gene ,Phylogeny ,Conserved Sequence ,Multidisciplinary ,Gene Expression Profiling ,Human Genome ,Chromosome ,Molecular ,Molecular Sequence Annotation ,biology.organism_classification ,Diploidy ,Tetraploidy ,030104 developmental biology ,Evolutionary biology ,Mutagenesis ,DNA Transposable Elements ,Female ,Molecular Developmental Biology ,030217 neurology & neurosurgery ,Gene Deletion ,Pseudogenes ,Biotechnology - Abstract
To explore the origins and consequences of tetraploidy in the African clawed frog, we sequenced the Xenopus laevis genome and compared it to the related diploid X. tropicalis genome. We characterize the allotetraploid origin of X. laevis by partitioning its genome into two homoeologous subgenomes, marked by distinct families of 'fossil' transposable elements. On the basis of the activity of these elements and the age of hundreds of unitary pseudogenes, we estimate that the two diploid progenitor species diverged around 34 million years ago (Ma) and combined to form an allotetraploid around 17-18 Ma. More than 56% of all genes were retained in two homoeologous copies. Protein function, gene expression, and the amount of conserved flanking sequence all correlate with retention rates. The subgenomes have evolved asymmetrically, with one chromosome set more often preserving the ancestral state and the other experiencing more gene loss, deletion, rearrangement, and reduced gene expression.
- Published
- 2016
27. VNAR single-domain antibodies specific for BAFF inhibit B cell development by molecular mimicry
- Author
-
Julien Häsler, Martin F. Flajnik, J Lynn Rutkowski, Frank S. Walsh, and Gareth Williams
- Subjects
0301 basic medicine ,Phage display ,Immunology ,medicine.disease_cause ,Article ,03 medical and health sciences ,Mice ,0302 clinical medicine ,B cell homeostasis ,Peptide Library ,Antibodies, Bispecific ,B-Cell Activating Factor ,Splenocyte ,medicine ,Animals ,Humans ,B-cell activating factor ,Receptor ,Molecular Biology ,B cell ,B-Lymphocytes ,biology ,Molecular Mimicry ,Single-Domain Antibodies ,Molecular biology ,Mice, Inbred C57BL ,Molecular mimicry ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,Antibody - Abstract
B cell-activating factor (BAFF) plays a dominant role in the B cell homeostasis. However, excessive BAFF promotes the development of autoreactive B-cells and several antibodies have been developed to block its activity. Bispecific antibodies with added functionality represent the next wave of biologics that may be more effective in the treatment of complex autoimmune disease. The single variable domain from the immunoglobulin new antigen receptor (VNAR) is one of the smallest antibody recognition units that could be combined with monospecific antibodies to develop bispecific agents. We isolated a panel of BAFF-binding VNARs with low nM potency from a semi-synthetic phage display library and examined their functional activity. The anti-BAFF VNARs blocked the binding of BAFF to all three of its receptors (BR3, TACI and BCMA) and the presence of the conserved DXL receptor motif found in the CDR3 regions suggests molecular mimicry as the mechanism of antagonism. One clone was formatted as an Fc fusion for functional testing and it was found to inhibit both mouse and human BAFF with equal potency ex vivo in a splenocyte proliferation assay. In mice, subchronic administration reduced the number of immature and transitional intermediates B cells and mature B cell subsets. These results indicate that VNAR single domain antibodies function as selective B-cell inhibitors and offer an alternative molecular format for targeting B-cell disorders.
- Published
- 2016
28. Shark class II invariant chain reveals ancient conserved relationships with cathepsins and MHC class II
- Author
-
Matthew D. Graham, Martin F. Flajnik, Yuko Ohta, Jeannine O. Eubanks, Patricia L. Chen, and Michael F. Criscitiello
- Subjects
Fish Proteins ,CD74 ,Genes, MHC Class II ,Molecular Sequence Data ,Immunology ,Major histocompatibility complex ,Article ,Evolution, Molecular ,Exon ,Animals ,Amino Acid Sequence ,Phylogeny ,Genetics ,Antigen Presentation ,MHC class II ,biology ,Antigen processing ,Alternative splicing ,Fishes ,Histocompatibility Antigens Class II ,Intron ,biology.organism_classification ,Cathepsins ,Antigens, Differentiation, B-Lymphocyte ,Sharks ,biology.protein ,Nurse shark ,Sequence Alignment ,Developmental Biology - Abstract
The invariant chain (Ii) is the critical third chain required for the MHC class II heterodimer to be properly guided through the cell, loaded with peptide, and expressed on the surface of antigen presenting cells. Here, we report the isolation of the nurse shark Ii gene, and the comparative analysis of Ii splice variants, expression, genomic organization, predicted structure, and function throughout vertebrate evolution. Alternative splicing to yield Ii with and without the putative protease-protective, thyroglobulin-like domain is as ancient as the MHC-based adaptive immune system, as our analyses in shark and lizard further show conservation of this mechanism in all vertebrate classes except bony fish. Remarkable coordinate expression of Ii and class II was found in shark tissues. Conserved Ii residues and cathepsin L orthologs suggest their long co-evolution in the antigen presentation pathway, and genomic analyses suggest 450 million years of conserved Ii exon/intron structure. Other than an extended linker preceding the thyroglobulin-like domain in cartilaginous fish, the Ii gene and protein are predicted to have largely similar physiology from shark to man. Duplicated Ii genes found only in teleosts appear to have become sub-functionalized, as one form is predicted to play the same role as that mediated by Ii mRNA alternative splicing in all other vertebrate classes. No Ii homologs or potential ancestors of any of the functional Ii domains were found in the jawless fish or lower chordates.
- Published
- 2012
29. The Multiple Shark Ig H Chain Genes Rearrange and Hypermutate Autonomously
- Author
-
Martin F. Flajnik, Ellen Hsu, Catherine Zhu, Pauline Hua, Yuko Ohta, Wendy Feng, Jeremy Weedon, and Dimitre G. Stefanov
- Subjects
Genetics ,Immunology ,Somatic hypermutation ,chemical and pharmacologic phenomena ,Cytidine deaminase ,Gene rearrangement ,Biology ,Molecular biology ,Isotype ,Exon ,medicine.anatomical_structure ,medicine ,Immunology and Allergy ,Immunoglobulin heavy chain ,Gene ,B cell - Abstract
Sharks and skates are representatives of the earliest vertebrates with an immune system based on V(D)J rearrangement. They possess a unique Ig gene organization consisting of 15 to >50 individual IgM loci, each with one VH, two DH, one JH, and one set of constant region exons. The present study attempts to understand how multiple Ig genes are regulated with respect to rearrangement initiation and to targeting during somatic hypermutation. The linkage of three single-copy IgH genes was determined, and single-cell genomic PCR studies in a neonatal animal were used to examine any relationship between relative gene position and likelihood of rearrangement. Our results show that one to three IgH genes are activated independently of linkage or allelic position and the data best fit with a probability model based on the hypothesis that V(D)J rearrangement occurs as a sequence of trials within the B cell. In the neonatal cell set, two closely related IgH, G2A, and G2B, rearranged at similar frequencies, and their membrane forms were expressed at similar levels, like in other young animals. However, older animals displayed a bias in favor of the G2A isotype, which suggests that although rearrangement at G2A and G2B was randomly initiated during primary repertoire generation, the two very similar IgM sequences appear to be differentially expressed with age and exposure to Ag. We performed genomic single-cell PCR on B cells from an immunized individual to study activation-induced cytidine deaminase targeting and found that hypermutation, like V(D)J rearrangement, occurred independently among the many shark IgH.
- Published
- 2011
30. Primordial Linkage of β2-Microglobulin to the MHC
- Author
-
Rebecca L. Lohr, Kazuyoshi Hosomichi, Martin F. Flajnik, Toni I. Pollin, Yuko Ohta, Hidetoshi Inoko, Shingo Suzuki, Takashi Shiina, and Edward J. Heist
- Subjects
Male ,CD74 ,Genetic Linkage ,Genes, MHC Class II ,Molecular Sequence Data ,Immunology ,Genes, MHC Class I ,chemical and pharmacologic phenomena ,Major histocompatibility complex ,Article ,Conserved sequence ,Mice ,Dogs ,MHC class I ,Animals ,Humans ,Immunology and Allergy ,Gene ,Conserved Sequence ,Phylogeny ,Zebrafish ,Genetics ,Bacterial artificial chromosome ,Base Sequence ,biology ,Beta-2 microglobulin ,Opossums ,biology.organism_classification ,Rats ,Sharks ,biology.protein ,Cattle ,Female ,Nurse shark ,beta 2-Microglobulin ,Chickens - Abstract
β2-Microglobulin (β2M) is believed to have arisen in a basal jawed vertebrate (gnathostome) and is the essential L chain that associates with most MHC class I molecules. It contains a distinctive molecular structure called a constant-1 Ig superfamily domain, which is shared with other adaptive immune molecules including MHC class I and class II. Despite its structural similarity to class I and class II and its conserved function, β2M is encoded outside the MHC in all examined species from bony fish to mammals, but it is assumed to have translocated from its original location within the MHC early in gnathostome evolution. We screened a nurse shark bacterial artificial chromosome library and isolated clones containing β2M genes. A gene present in the MHC of all other vertebrates (ring3) was found in the bacterial artificial chromosome clone, and the close linkage of ring3 and β2M to MHC class I and class II genes was determined by single-strand conformational polymorphism and allele-specific PCR. This study satisfies the long-held conjecture that β2M was linked to the primordial MHC (Ur MHC); furthermore, the apparent stability of the shark genome may yield other genes predicted to have had a primordial association with the MHC specifically and with immunity in general.
- Published
- 2011
31. Emergence of the acute-phase protein hemopexin in jawed vertebrates
- Author
-
E. Bryan Buckingham, Martin F. Flajnik, Michael F. Criscitiello, and Helen Dooley
- Subjects
Molecular Sequence Data ,Immunology ,Inflammation ,Leucoraja erinacea ,Article ,chemistry.chemical_compound ,Hemopexin ,medicine ,Animals ,Amino Acid Sequence ,Skates, Fish ,Molecular Biology ,Heme ,Phylogeny ,chemistry.chemical_classification ,biology ,Fishes ,Acute-phase protein ,Blotting, Northern ,biology.organism_classification ,Biological Evolution ,chemistry ,Biochemistry ,Sharks ,Hemoglobin ,medicine.symptom ,Nurse shark ,Glycoprotein ,Sequence Alignment - Abstract
When released from damaged erythrocytes free heme not only provides a source of iron for invading bacteria but also highly toxic due to its ability to catalyze free radical formation. Hemopexin (Hx) binds free heme with very high-affinity and thus protects against heme toxicity, sequesters heme from pathogens, and helps conserve valuable iron. Hx is also an acute-phase serum protein (APP), whose expression is induced by inflammation. To date Hx has been identified as far back in phylogeny as bony fish where it is called warm-temperature acclimation-related 65 kDa protein (WAP65), as serum protein levels are increased at elevated environmental temperatures as well as by infection. During analysis of nurse shark (Ginglymostoma cirratum) plasma we isolated a Ni 2+ -binding serum glycoprotein and characterized it as the APP Hx. We subsequently cloned Hx from nurse shark and another cartilaginous fish species, the little skate Leucoraja erinacea. Functional analysis showed shark Hx, like that of mammals, binds heme but is found at unusually high levels in normal shark serum. As an Hx orthologue could not be found in the genomes of jawless vertebrates or lower deuterostomes it appears to have arisen just prior to the emergence of jawed vertebrates, coincident with the second round of genome-wide duplication and the appearance of tetrameric hemoglobin (Hb). Published by Elsevier Ltd.
- Published
- 2010
32. Evolutionarily Conserved TCR Binding Sites, Identification of T Cells in Primary Lymphoid Tissues, and Surprising Trans-Rearrangements in Nurse Shark
- Author
-
Yuko Ohta, E. Churchill McKinney, Martin F. Flajnik, Mark Saltis, and Michael F. Criscitiello
- Subjects
Lymphoid Tissue ,T-Lymphocytes ,T cell ,Gene Rearrangement, delta-Chain T-Cell Antigen Receptor ,Molecular Sequence Data ,Immunology ,Receptors, Antigen, T-Cell ,Gene Expression ,chemical and pharmacologic phenomena ,Biology ,Article ,Conserved sequence ,medicine ,Animals ,Humans ,Immunology and Allergy ,Amino Acid Sequence ,Northern blot ,Gene ,Conserved Sequence ,In Situ Hybridization ,Phylogeny ,Southern blot ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,T-cell receptor ,hemic and immune systems ,Blotting, Northern ,biology.organism_classification ,Molecular biology ,Blotting, Southern ,medicine.anatomical_structure ,Sharks ,Paratope ,Nurse shark ,Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor ,human activities - Abstract
Cartilaginous fish are the oldest animals that generate RAG-based Ag receptor diversity. We have analyzed the genes and expressed transcripts of the four TCR chains for the first time in a cartilaginous fish, the nurse shark (Ginglymostoma cirratum). Northern blotting found TCR mRNA expression predominantly in lymphoid and mucosal tissues. Southern blotting suggested translocon-type loci encoding all four chains. Based on diversity of V and J segments, the expressed combinatorial diversity for gamma is similar to that of human, alpha and beta may be slightly lower, and delta diversity is the highest of any organism studied to date. Nurse shark TCRdelta have long CDR3 loops compared with the other three chains, creating binding site topologies comparable to those of mammalian TCR in basic paratope structure; additionally, nurse shark TCRdelta CDR3 are more similar to IgH CDR3 in length and heterogeneity than to other TCR chains. Most interestingly, several cDNAs were isolated that contained IgM or IgW V segments rearranged to other gene segments of TCRdelta and alpha. Finally, in situ hybridization experiments demonstrate a conservation of both alpha/beta and gamma/delta T cell localization in the thymus across 450 million years of vertebrate evolution, with gamma/delta TCR expression especially high in the subcapsular region. Collectively, these data make the first cellular identification of TCR-expressing lymphocytes in a cartilaginous fish.
- Published
- 2010
33. Somatic hypermutation of TCRα contributes to thymic positive selection in sharks
- Author
-
Jeannine A. Ott, Caitlin Castro, Thaddeus Deiss, Yuko Ota, Martin F. Flajnik, and Michael F. Criscitiello
- Subjects
Immunology ,Immunology and Allergy - Abstract
In mammals and probably all vertebrates, receptor editing of TCR alpha genes enhances immature T cell positive selection over a three-day interval in the thymic cortex. Surprisingly we found extensive somatic hypermutation (SHM) operating at the TCRα locus in the nurse shark thymus, implying that SHM contributes to receptor modifications that enhance positive selection. We analyzed mutation in TCRα families of clones with the same VJ rearrangement. Additionally, in situ hybridization showed the strongest activation-induced cytidine deaminase (AID) expression in the central thymic cortex and bordering the corticomedullary junction. The frequency of mutation at TCRα was as high as that seen at B cell receptor (BCR) loci in sharks and mammals. Complementarity determining regions (CDRs) accumulated significantly more mutations than framework regions (FWs), and significantly more mutations in CDRs resulted in amino acid replacement than in silent mutation. We saw a preference for transition mutations as well as a strong bias toward G:C substitutions within AID hotspots, especially within CDR regions. We suggest that TCRα utilizes SHM to boost positive selection and perhaps to broaden diversification of the αβ T cell repertoire in sharks, the first reported use of this process in thymic diversification in vertebrates.
- Published
- 2018
34. Origin and evolution of the adaptive immune system: genetic events and selective pressures
- Author
-
Martin F. Flajnik and Masanori Kasahara
- Subjects
Receptors, Antigen, T-Cell ,Immunoglobulins ,Receptors, Antigen, B-Cell ,Article ,Major Histocompatibility Complex ,Immune system ,Variable lymphocyte receptor ,Molecular evolution ,Convergent evolution ,biology.animal ,Gene duplication ,Genetics ,Animals ,Humans ,Cell Lineage ,Lymphocytes ,Selection, Genetic ,Somatic recombination ,Molecular Biology ,Genetics (clinical) ,Recombination, Genetic ,Models, Genetic ,biology ,Fishes ,Immunity ,Vertebrate ,Acquired immune system ,Immune System ,Vertebrates - Abstract
The adaptive immune system (AIS) in mammals, which is centred on lymphocytes bearing antigen receptors that are generated by somatic recombination, arose approximately 500 million years ago in jawed fish. This intricate defence system consists of many molecules, mechanisms and tissues that are not present in jawless vertebrates. Two macroevolutionary events are believed to have contributed to the genesis of the AIS: the emergence of the recombination-activating gene (RAG) transposon, and two rounds of whole-genome duplication. It has recently been discovered that a non-RAG-based AIS with similarities to the jawed vertebrate AIS - including two lymphoid cell lineages - arose in jawless fish by convergent evolution. We offer insights into the latest advances in this field and speculate on the selective pressures that led to the emergence and maintenance of the AIS.
- Published
- 2009
35. High-affinity lamprey VLRA and VLRB monoclonal antibodies
- Author
-
S. Annie Gai, Roy A. Mariuzza, K. Dane Wittrup, Zeev Pancer, Gang Xu, C. Alejandro Velikovsky, Satoshi Tasumi, and Martin F. Flajnik
- Subjects
medicine.drug_class ,Protein subunit ,Molecular Sequence Data ,Antibody Affinity ,Leucine-Rich Repeat Proteins ,Monoclonal antibody ,Immune system ,Antigen ,Variable lymphocyte receptor ,medicine ,Animals ,Multidisciplinary ,Base Sequence ,biology ,Lamprey ,Antibodies, Monoclonal ,Lampreys ,Proteins ,Biological Sciences ,biology.organism_classification ,Acquired immune system ,Adaptation, Physiological ,Molecular biology ,Receptors, Antigen ,Biochemistry ,biology.protein ,Antibody - Abstract
Lamprey are members of the ancestral vertebrate taxon (jawless fish), which evolved rearranging antigen receptors convergently with the jawed vertebrates. But instead of Ig superfamily domains, lamprey variable lymphocyte receptors (VLRs) consist of highly diverse leucine-rich repeats. Although VLRs represent the only known adaptive immune system not based on Ig, little is known about their antigen-binding properties. Here we report robust plasma VLRB responses of lamprey immunized with hen egg lysozyme and β-galactosidase (β-gal), demonstrating adaptive immune responses against soluble antigens. To isolate monoclonal VLRs, we constructed large VLR libraries from antigen-stimulated and naïve animals in a novel yeast surface-display vector, with the VLR C-terminally fused to the yeast Flo1p surface anchor. We cloned VLRB binders of lysozyme, β-gal, cholera toxin subunit B, R-phycoerythrin, and B-trisaccharide antigen, with dissociation constants up to the single-digit picomolar range, equivalent to those of high-affinity IgG antibodies. We also isolated from a single lamprey 13 anti-lysozyme VLRA clones with affinities ranging from low nanomolar to mid-picomolar. All of these VLRA clones were closely related in sequence, differing at only 15 variable codon positions along the 244-residue VLR diversity region, which augmented antigen-binding affinity up to 100-fold. Thus, VLRs can provide a protective humoral antipathogen shield. Furthermore, the broad range of nominal antigens that VLRs can specifically bind, and the affinities achieved, indicate a functional parallelism between LRR-based and Ig-based antibodies. VLRs may be useful natural single-chain alternatives to conventional antibodies for biotechnology applications.
- Published
- 2009
36. Evolutionarily conserved and divergent regions of the Autoimmune Regulator (Aire) gene: a comparative analysis
- Author
-
Martin F. Flajnik, Yuko Ohta, Nikolaus S. Trede, Michael F. Criscitiello, Matthew D. Keefe, Mark Saltis, and Ryo Goitsuka
- Subjects
T-Lymphocytes ,T cell ,Molecular Sequence Data ,Immunology ,Article ,Evolution, Molecular ,Mice ,Xenopus laevis ,biology.animal ,Genetics ,medicine ,Ring finger ,Animals ,Humans ,Amino Acid Sequence ,RNA, Messenger ,Zebrafish ,Gene ,Transcription factor ,Conserved Sequence ,Phylogeny ,Sequence Homology, Amino Acid ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Point mutation ,Genetic Variation ,Vertebrate ,Blotting, Northern ,biology.organism_classification ,Autoimmune regulator ,Protein Structure, Tertiary ,DNA-Binding Proteins ,medicine.anatomical_structure ,Chickens ,Transcription Factors - Abstract
During T cell differentiation, medullary thymic epithelial cells (MTEC) expose developing T cells to tissue-specific antigens. MTEC expression of such self-antigens requires the transcription factor autoimmune regulator (Aire). In mammals, defects in aire result in multi-tissue, T cell-mediated autoimmunity. Because the T cell receptor repertoire is randomly generated and extremely diverse in all jawed vertebrates, it is likely that an aire-dependent T cell tolerance mechanism also exists in nonmammalian vertebrates. We have isolated aire genes from animals in all gnathostome classes except the cartilaginous fish by a combination of molecular techniques and scanning of expressed sequence tags and genomic databases. The deduced amino acid sequences of Aire were compared among mouse, human, opossum, chicken, Xenopus, zebrafish, and pufferfish. The first of two plant homeodomains (PHD) in human Aire and regions associated with nuclear and cytoplasmic localization are evolutionarily conserved, while other domains are either absent or divergent in one or more vertebrate classes. Furthermore, the second zinc-binding domain previously named Aire PHD2 appears to have greater sequence similarity with Ring finger domains than to PHD domains. Point mutations in defective human aire genes are generally found in the most evolutionarily conserved regions of the protein. These findings reveal a very rapid evolution of certain regions of aire during vertebrate evolution and support the existence of an aire-dependent mechanism of T cell tolerance dating back at least to the emergence of bony fish.
- Published
- 2008
37. Four primordial immunoglobulin light chain isotypes, including λ and κ, identified in the most primitive living jawed vertebrates
- Author
-
Martin F. Flajnik and Michael F. Criscitiello
- Subjects
Molecular Sequence Data ,Immunology ,Xenopus ,Immunoglobulin light chain ,Article ,Immunoglobulin kappa-Chains ,Xenopus laevis ,Immunoglobulin lambda-Chains ,Phylogenetics ,biology.animal ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Skates, Fish ,Peptide sequence ,Phylogeny ,Phylogenetic tree ,biology ,Vertebrate ,Anatomy ,biology.organism_classification ,Isotype ,Ictaluridae ,Immunoglobulin Isotypes ,Dogfish ,Evolutionary biology ,Sharks ,biology.protein ,Antibody - Abstract
The discovery of a fourth immunoglobulin (Ig) light (L) chain isotype in sharks has revealed the origins and natural history of all vertebrate L chains. Phylogenetic comparisons have established orthology between this new shark L chain and the unique Xenopus L chain isotype sigma. More importantly, inclusion of this new L chain family in phylogenetic analyses showed that all vertebrate L chains can be categorized into four ancestral clans originating prior to the emergence of cartilaginous fish: one restricted to elasmobranchs (sigma-cart/type I), one found in all cold-blooded vertebrates (sigma/teleost type 2/elasmobranch type IV), one in all groups except bony fish (lambda/elasmobranch type II), and one in all groups except birds (kappa/elasmobranch type III/teleost type 1 and 3). All four of these primordial L chain isotypes (sigma, sigma-cart, lambda and kappa) have maintained separate V region identities since their emergence at least 450 million years ago, suggestive of an ancient physiological distinction of the L chains. We suggest that, based upon unique, discrete sizes of complementarity determining regions 1 and 2 and other features of the V region sequences, the different L chain isotypes arose to provide different functional conformations in the Ig binding site when they pair with heavy chains.
- Published
- 2007
38. Biased Immunoglobulin Light Chain Gene Usage in the Shark
- Author
-
Ellen Hsu, Ushma Naik, Martin F. Flajnik, Anita Lui, Yuko Ohta, and Anna Iacoangeli
- Subjects
Genetics ,Fish Proteins ,biology ,Immunology ,V(D)J recombination ,Receptor editing ,Gene rearrangement ,biology.organism_classification ,Immunoglobulin light chain ,Molecular biology ,Germline ,V(D)J Recombination ,Article ,Sharks ,Immunology and Allergy ,Animals ,Gene Rearrangement, B-Lymphocyte, Light Chain ,Immunoglobulin Light Chains ,Nurse shark ,Gene ,Minigene - Abstract
This study of a large family of kappa light (L) chain clusters in nurse shark completes the characterization of its classical immunoglobulin (Ig) gene content (two heavy chain classes, mu and omega, and four L chain isotopes, kappa, lambda, sigma, and sigma-2). The shark kappa clusters are minigenes consisting of a simple VL-JL-CL array, where V to J recombination occurs over a ~500 bp interval, and functional clusters are widely separated by at least 100 kb. Six out of ca. 39 kappa clusters are pre-rearranged in the germline (GL-joined). Unlike the complex gene organization and multistep assembly process of Ig in mammals, each shark Ig rearrangement, somatic or in the germline, appears to be an independent event localized to the minigene. This study examined the expression of functional, non-productive, and sterile transcripts of the kappa clusters compared to the other three L chain isotypes. Kappa cluster usage was investigated in young sharks, and a skewed pattern of split gene expression was observed, one similar in functional and non-productive rearrangements. These results show that the individual activation of the spatially distant kappa clusters is non-random. Although both split and GL-joined kappa genes are expressed, the latter are prominent in young animals and wane with age. We speculate that, in the shark, the differential activation of the multiple isotypes can be advantageously used in receptor editing.
- Published
- 2015
39. The plasticity of immunoglobulin gene systems in evolution
- Author
-
Ellen Hsu, Martin F. Flajnik, Nicolas Pulham, and Lynn L. Rumfelt
- Subjects
Immunoglobulin gene ,Genetics ,Base Sequence ,Genes, Immunoglobulin ,Pseudogene ,Molecular Sequence Data ,Immunology ,Immunoglobulin Variable Region ,Immunoglobulins ,Gene rearrangement ,Biology ,Article ,Evolution, Molecular ,Mice ,Molecular evolution ,Recombinase ,Animals ,Immunology and Allergy ,Recombination signal sequences ,Gene conversion ,Gene Rearrangement, B-Lymphocyte ,Immunoglobulin Constant Regions ,Gene ,Phylogeny - Abstract
The mechanism of recombination-activating gene (RAG)-mediated rearrangement exists in all jawed vertebrates, but the organization and structure of immunoglobulin (Ig) genes, as they differ in fish and among fish species, reveal their capability for rapid evolution. In systems where there can exist 100 Ig loci, exon restructuring and sequence changes of the constant regions led to divergence of effector functions. Recombination among these loci created hybrid genes, the strangest of which encode variable (V) regions that function as part of secreted molecules and, as the result of an ancient translocation, are also grafted onto the T-cell receptor. Genomic changes in V-gene structure, created by RAG recombinase acting on germline recombination signal sequences, led variously to the generation of fixed receptor specificities, pseudogene templates for gene conversion, and ultimately to Ig sequences that evolved away from Ig function. The presence of so many Ig loci in fishes raises interesting questions not only as to how their regulation is achieved but also how successive whole-locus duplications are accommodated by a system whose function in other vertebrates is based on clonal antigen receptor expression.
- Published
- 2006
40. An evolutionarily mobile antigen receptor variable region gene: Doubly rearranging NAR-TcR genes in sharks
- Author
-
Martin F. Flajnik, Michael F. Criscitiello, and Mark Saltis
- Subjects
Lymphoid Tissue ,Molecular Sequence Data ,Sequence alignment ,Locus (genetics) ,Biology ,Conserved sequence ,Evolution, Molecular ,Antigen ,Animals ,Humans ,Amino Acid Sequence ,Gene ,Conserved Sequence ,Phylogeny ,Gene Rearrangement ,Genetics ,Regulation of gene expression ,Genome ,Multidisciplinary ,T-cell receptor ,Genetic Variation ,Receptors, Antigen, T-Cell, gamma-delta ,Gene rearrangement ,Biological Sciences ,Gene Expression Regulation ,Organ Specificity ,Sharks ,Sequence Alignment - Abstract
Distinctive Ig and T cell receptor (TcR) chains define the two major lineages of vertebrate lymphocyte yet similarly recognize antigen with a single, membrane-distal variable (V) domain. Here we describe the first antigen receptor chain that employs two V domains, which are generated by separate VDJ gene rearrangement events. These molecules have specialized “supportive” TcRδV domains membrane-proximal to domains with most similarity to IgNAR V. The ancestral NAR V gene encoding this domain is hypothesized to have recombined with theTRDlocus in a cartilaginous fish ancestor >200 million years ago and encodes the first V domain shown to be used in both Igs and TcRs. Furthermore, these data support the view that γ/δ TcRs have for long used structural conformations recognizing free antigen.
- Published
- 2006
41. First molecular and biochemical analysis ofin vivoaffinity maturation in an ectothermic vertebrate
- Author
-
Martin F. Flajnik, Rebecca A. Brady, Robyn L. Stanfield, and Helen Dooley
- Subjects
Fish Proteins ,Models, Molecular ,Genetics ,Multidisciplinary ,Molecular Sequence Data ,Sequence alignment ,Context (language use) ,Biological Sciences ,Biology ,Immunoglobulin light chain ,Isotype ,Body Temperature ,Protein Structure, Tertiary ,Affinity maturation ,Germ Cells ,Antigen ,Sharks ,Animals ,Amino Acid Sequence ,Clone (B-cell biology) ,Sequence Alignment ,Gene ,Body Temperature Regulation - Abstract
The cartilaginous fish are the oldest phylogenetic group in which Igs have been found. Sharks produce a unique Ig isotype, IgNAR, a heavy-chain homodimer that does not associate with light chains. Instead, the variable (V) regions of IgNAR bind antigen as soluble single domains. Our group has shown that IgNAR plays an integral part in the humoral response of nurse sharks (Ginglymostoma cirratum) upon antigen challenge. Here, we generated phage-displayed libraries of IgNAR V regions from an immunized animal and found a family of clones derived from the same rearrangement event but differentially mutated during expansion. Because of the cluster organization of shark Ig genes and the paucicopy nature of IgNAR, we were able to construct the putative ancestor of this family. By studying mutations in the context of clone affinities, we found evidence that affinity maturation occurs for this isotype. Subsequently, we were able to identify mutations important in the affinity improvement of this family. Because the family clones were all obtained after immunization, they provide insight into thein vivomaturation mechanisms, in general, and for single-domain antibody fragments.
- Published
- 2006
42. Antibody repertoire development in cartilaginous fish
- Author
-
Martin F. Flajnik and Helen Dooley
- Subjects
Genetics ,Mutation ,Immunoprecipitation ,Ontogeny ,Molecular Sequence Data ,Immunology ,Fishes ,Biology ,medicine.disease_cause ,Molecular biology ,Germline ,Immunoglobulin Isotypes ,Species Specificity ,Antigen ,Antibody Repertoire ,Antibody Formation ,biology.protein ,medicine ,Animals ,Amino Acid Sequence ,Antibody ,Gene ,Developmental Biology - Abstract
There are 3 H chain and 3 L chain isotypes in the cartilaginous fish, all encoded by genes in the so-called cluster (VDDJ, VJ) organization. The H chain isotypes IgM and IgNAR, are readily detected at the protein level in most species. The third is readily identified at the protein level in skates (IgR) but only via immunoprecipitation or at the transcript level in sharks (IgW). High levels of diversity in CDR3 and up to 200 germline genes have been detected for IgM depending upon the species examined. IgNAR displays very high levels of CDR3 diversity but almost none in the germline. At least IgNAR and L chain genes have been shown to hypermutate to very high levels, apparently in response to antigen. The mutation footprints are similar to those in mammals except that the shark genes uniquely mutate nucleotide residues in tandem. A conspicuous feature of cartilaginous fish Ig genes is the presence of germline-joined genes, which are a result of RAG activity in germ cells. Such genes are expressed early in ontogeny and then extinguished or expressed at lower levels. 19S IgM and IgW expression precede that of 7S IgM and IgNAR during ontogeny. The ‘switch’ from 19S to 7S IgM, the regulation of expression of the Ig clusters, and the microenvironments for mutation/selection of cartilaginous fish B cells are all areas of ongoing research.
- Published
- 2006
43. Re-evaluation of the immunological Big Bang
- Author
-
Martin F. Flajnik
- Subjects
Genetics ,Agricultural and Biological Sciences(all) ,biology ,Biochemistry, Genetics and Molecular Biology(all) ,Lineage (evolution) ,Lamprey ,Adaptation, Biological ,Lampreys ,Adaptive Immunity ,biology.organism_classification ,Acquired immune system ,General Biochemistry, Genetics and Molecular Biology ,Article ,Evolution, Molecular ,Immune system ,biology.animal ,Vertebrates ,Recombinase ,Immunoglobulin superfamily ,Animals ,General Agricultural and Biological Sciences ,Gene ,Hagfish - Abstract
Classically the immunological ‘Big Bang’ of adaptive immunity was believed to have resulted from the insertion of a transposon into an immunoglobulin superfamily gene member, initiating antigen receptor gene rearrangement via the RAG recombinase in an ancestor of jawed vertebrates. However, the discovery of a second, convergent adaptive immune system in jawless fish, focused on the so-called variable lymphocyte receptors (VLRs), was arguably the most exciting finding of the past decade in immunology and has drastically changed the view of immune origins. The recent report of a new lymphocyte lineage in lampreys, defined by the antigen receptor VLRC, suggests that there were three lymphocyte lineages in the common ancestor of jawless and jawed vertebrates that co-opted different antigen receptor supertypes. The transcriptional control of these lineages during development is predicted to be remarkably similar in both the jawless (agnathan) and jawed (gnathostome) vertebrates, suggesting that an early ‘division of labor’ among lymphocytes was a driving force in the emergence of adaptive immunity. The recent cartilaginous fish genome project suggests that most effector cytokines and chemokines were also present in these fish, and further studies of the lamprey and hagfish genomes will determine just how explosive the Big Bang actually was.
- Published
- 2014
44. Immunobiology of the SHARK
- Author
-
Martin F. Flajnik, Robert B. Sim, and Sylvia L. Smith
- Subjects
Biology - Published
- 2014
45. Somatic Hypermutation and Junctional Diversification at Ig Heavy Chain Loci in the Nurse Shark
- Author
-
Jennie E. Brodsky, Ellen Hsu, Yuko Ohta, Karolina Malecek, Julie Brandman, and Martin F. Flajnik
- Subjects
Genetic Markers ,Recombination, Genetic ,Genetics ,cDNA library ,Molecular Sequence Data ,Immunology ,Somatic hypermutation ,Locus (genetics) ,Biology ,biology.organism_classification ,Complementarity Determining Regions ,Evolution, Molecular ,Restriction enzyme ,Complementary DNA ,Mutation ,Sharks ,Animals ,Immunology and Allergy ,Immunoglobulin heavy chain ,Immunoglobulin Light Chains ,Amino Acid Sequence ,Somatic Hypermutation, Immunoglobulin ,Nurse shark ,Immunoglobulin Heavy Chains ,Gene - Abstract
We estimate there are ∼15 IgM H chain loci in the nurse shark genome and have characterized one locus. It consists of one V, two D, and one J germline gene segments, and the constant (C) region can be distinguished from all of the others by a unique combination of restriction endonuclease sites in Cμ2. On the basis of these Cμ2 markers, 22 cDNA clones were selected from an epigonal organ cDNA library from the same individual; their C region sequences proved to be the same up to the polyadenylation site. With the identification of the corresponding germline gene segments, CDR3 from shark H chain rearrangements could be analyzed precisely, for the first time. Considerable diversity was generated by trimming and N addition at the three junctions and by varied recombination patterns of the two D gene segments. The cDNA sequences originated from independent rearrangements events, and most carried both single and contiguous substitutions. The 53 point mutations occurred with a bias for transition changes (53%), whereas the 78 tandem substitutions, mostly 2–4 bp long, do not (36%). The nature of the substitution patterns is the same as for mutants from six loci of two nurse shark L chain isotypes, showing that somatic hypermutation events are very similar at both H and L chain genes in this early vertebrate. The cis-regulatory elements targeting somatic hypermutation must have already existed in the ancestral Ig gene, before H and L chain divergence.
- Published
- 2005
46. Cloning and characterization of ?2-microglobulin
- Author
-
John D. Horton, Rebecca Stewart, Ralph R. Minter, Pamela Ritchie, Yuko Ohta, Trudy L. Horton, Martin F. Flajnik, Martin D. Watson, and Terry Gibbons
- Subjects
Messenger RNA ,biology ,urogenital system ,Immunology ,Xenopus ,Locus (genetics) ,biology.organism_classification ,Major histocompatibility complex ,Molecular biology ,Complementary DNA ,MHC class I ,biology.protein ,Gene ,Peptide sequence ,Developmental Biology - Abstract
cDNAs for Xenopus β2-microglobulin (β2m), the obligatory light chain of most vertebrate Major Histocompatibility Complex (MHC) class I molecules, were isolated and ESTs were identified. Alignment of the deduced amino acid sequence to other species' β2m showed that the overall structure is evolutionarily conserved, and phylogenetic analysis showed that the Xenopus β2m sequence is intermediate between fish and bird/mammal β2m. The Xenopus β2m mRNA is expressed ubiquitously with highest expression in intestine, spleen, and thymus, correlating well with classical class Ia expression. β2m mRNA and protein were also detected in Xenopus thymic tumor and kidney cell lines. Segregation analysis on a tetraploid Xenopus laevis family revealed two independently segregating, non-MHC-linked loci. As expected, only one locus was found in the diploid Xenopus tropicalis, strongly suggesting that the two β2m loci in the tetraploid species were generated by genome-wide duplication, and did not undergo diploidization unlike many other MHC genes.
- Published
- 2005
47. Evolution of innate and adaptive immunity: can we draw a line?
- Author
-
Martin F. Flajnik and Louis Du Pasquier
- Subjects
Innate immune system ,Mechanism (biology) ,animal diseases ,Immunology ,chemical and pharmacologic phenomena ,Gene rearrangement ,biochemical phenomena, metabolism, and nutrition ,Biology ,Acquired immune system ,Major histocompatibility complex ,Adaptation, Physiological ,Biological Evolution ,Invertebrates ,Immunity, Innate ,Major Histocompatibility Complex ,Immune system ,Immunity ,Vertebrates ,biology.protein ,Animals ,Humans ,bacteria ,Immunology and Allergy ,Adaptation - Abstract
Several recent findings in the field of comparative immunology have reinforced the importance of examining the molecular and functional features of immune systems in a variety of organisms. Particularly exciting are the discoveries of a new gene rearrangement mechanism in lampreys and a somatic diversification of mollusk immune genes. These immune features being found in animals previously believed only to have innate immunity, as well as the flood of information on immune genes, molecules and mechanisms in many different creatures, have prompted us to revisit the artificial dichotomy between adaptive and innate immune systems. Although we draw no startling conclusions, we hope to encourage different thought patterns when viewing immune systems.
- Published
- 2004
48. Shark Ig Light Chain Junctions Are as Diverse as in Heavy Chains
- Author
-
Martin F. Flajnik, Marshall Fleurant, Chin-Tung Chen, Ellen Hsu, and Lily Changchien
- Subjects
Genetic Markers ,5' Flanking Region ,Pseudogene ,Molecular Sequence Data ,Immunology ,Gene Rearrangement, B-Lymphocyte, Heavy Chain ,Locus (genetics) ,Biology ,Immunoglobulin light chain ,Exon ,Species Specificity ,Complementary DNA ,Animals ,Gene Rearrangement, B-Lymphocyte, Light Chain ,Immunology and Allergy ,3' Flanking Region ,RNA Processing, Post-Transcriptional ,Genetics ,Genomic Library ,Base Sequence ,Gene rearrangement ,biology.organism_classification ,Complementarity Determining Regions ,Junctional diversity ,Immunoglobulin Isotypes ,Sharks ,Immunoglobulin Joining Region ,Immunoglobulin Light Chains ,Nurse shark ,Immunoglobulin Heavy Chains ,Antibody Diversity - Abstract
We have characterized a small family of four genes encoding one of the three nurse shark Ig L chain isotypes, called NS5. All NS5 cDNA sequences are encoded by three loci, of which two are organized as conventional clusters, each consisting of a V and J gene segment that can recombine and one C region exon; the third contains a germline-joined VJ in-frame and the fourth locus is a pseudogene. This is the second nurse shark L chain type where both germline-joined and split V-J organizations have been found. Since there are only two rearranging Ig loci, it was possible for the first time to examine junctional diversity in defined fish Ig genes, comparing productive vs nonproductive rearrangements. N region addition was found to be considerably more extensive in length and in frequency than any other vertebrate L chain so far reported and rivals that in H chain. We put forth the speculation that the unprecedented efficiency of N region addition (87–93% of NS5 sequences) may be a result not only of simultaneous H and L chain rearrangement in the shark but also of processing events that afford greater accessibility of the V or J gene coding ends to terminal deoxynucleotidyltransferase.
- Published
- 2004
49. Crystal Structure of a Shark Single-Domain Antibody V Region in Complex with Lysozyme
- Author
-
Martin F. Flajnik, Ian A. Wilson, Robyn L. Stanfield, and Helen Dooley
- Subjects
Models, Molecular ,Protein Folding ,Protein Conformation ,Immunoglobulin Variable Region ,Immunoglobulins ,Complementarity determining region ,Immunoglobulin domain ,Biology ,Crystallography, X-Ray ,Evolution, Molecular ,Meglumine ,Protein structure ,Antigen ,Variable lymphocyte receptor ,Animals ,Multidisciplinary ,Genes, Immunoglobulin ,Tetrahydropapaveroline ,Complementarity Determining Regions ,Protein Structure, Tertiary ,Drug Combinations ,Receptors, Antigen ,Single-domain antibody ,Biochemistry ,Sharks ,biology.protein ,Biophysics ,Immunoglobulin heavy chain ,Muramidase ,Antibody ,Immunoglobulin Heavy Chains ,Dimerization - Abstract
Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
- Published
- 2004
50. CD1, MR1, NKT, and MAIT: evolution and origins of non-peptidic antigen recognition by T lymphocytes
- Author
-
Martin F. Flajnik and Dirk M. Zajonc
- Subjects
0301 basic medicine ,Receptors, Antigen, T-Cell, alpha-beta ,Immunology ,Antigen presentation ,CD1 ,Gene Expression ,Mucosal associated invariant T cell ,Biology ,Lymphocyte Activation ,Non-peptidic antigen ,Mucosal-Associated Invariant T Cells ,Article ,Antigens, CD1 ,Minor Histocompatibility Antigens ,03 medical and health sciences ,Genetics ,Minor histocompatibility antigen ,Animals ,Humans ,Antigen Presentation ,Histocompatibility Antigens Class I ,Biological evolution ,Biological Evolution ,Immunity, Innate ,Killer Cells, Natural ,030104 developmental biology ,Lymphocyte activation ,Glycolipids - Published
- 2016
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