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Ancient Use of Ig Variable Domains Contributes Significantly to the TCRδ Repertoire
- Source :
- The Journal of Immunology. 203:1265-1275
- Publication Year :
- 2019
- Publisher :
- The American Association of Immunologists, 2019.
-
Abstract
- The loci encoding B and T cell Ag receptors are generally distinct in commonly studied mammals, with each receptor’s gene segments limited to intralocus, cis chromosomal rearrangements. The nurse shark (Ginglymostoma cirratum) represents the oldest vertebrate class, the cartilaginous fish, with adaptive immunity provided via Ig and TCR lineages, and is one species among a growing number of taxa employing Ig-TCRδ rearrangements that blend these distinct lineages. Analysis of the nurse shark Ig-TCRδ repertoire found that these rearrangements possess CDR3 characteristics highly similar to canonical TCRδ rearrangements. Furthermore, the Ig-TCRδ rearrangements are expressed with TCRγ, canonically found in the TCRδ heterodimer. We also quantified BCR and TCR transcripts in the thymus for BCR (IgHV-IgHC), chimeric (IgHV-TCRδC), and canonical (TCRδV-TCRδC) transcripts, finding equivalent expression levels in both thymus and spleen. We also characterized the nurse shark TCRαδ locus with a targeted bacterial artifical chromosome sequencing approach and found that the TCRδ locus houses a complex of V segments from multiple lineages. An IgH-like V segment, nestled within the nurse shark TCRδ translocus, grouped with IgHV-like rearrangements we found expressed with TCRδ (but not IgH) rearrangements in our phylogenetic analysis. This distinct lineage of TCRδ-associated IgH-like V segments was termed “TAILVs.” Our data illustrate a dynamic TCRδ repertoire employing TCRδVs, NARTCRVs, bona fide trans-rearrangements from shark IgH clusters, and a novel lineage in the TCRδ-associated Ig-like V segments.
- Subjects :
- Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
Immunology
Immunoglobulin Variable Region
chemical and pharmacologic phenomena
Locus (genetics)
Article
03 medical and health sciences
0302 clinical medicine
Phylogenetics
Animals
Immunology and Allergy
Amino Acid Sequence
Phylogeny
biology
Repertoire
T-cell receptor
breakpoint cluster region
Chromosome
Receptors, Antigen, T-Cell, gamma-delta
Gene rearrangement
biology.organism_classification
Evolutionary biology
Sharks
Immunoglobulin Domains
Nurse shark
Sequence Alignment
030215 immunology
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....a9d882582c13bba6a5baff31896ea1a9