45 results on '"Huang, Qingde"'
Search Results
2. Solvent-free encapsulation of β-carotene in natural flaxseed oil bodies induced via tepidity-physical field treatment: Formation, characteristic and stability
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Zhang, Shan, Chen, Hongjian, Geng, Fang, Xie, Bijun, Sun, Zhida, Huang, Qingde, Peng, Dengfeng, Chen, Yashu, and Deng, Qianchun
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- 2023
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3. Targeted microbiome metabolomics reveals flaxseed oil supplementation regulated the gut microbiota and farnesoid X receptor pathway in high-fat diet mice
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Yang, Chen, Xu, Zhenxia, Huang, Qingde, Wang, Xu, Huang, Fenghong, and Deng, Qianchun
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- 2023
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4. Docosahexaenoic acid enhances hippocampal insulin sensitivity to promote cognitive function of aged rats on a high-fat diet
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Xu, Jiqu, Ni, Ben, Ma, Congcong, Rong, Shuang, Gao, Hui, Zhang, Li, Xiang, Xia, Huang, Qingde, Deng, Qianchun, and Huang, Fenghong
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- 2023
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5. Physical and emulsifying properties of pea protein: influence of combined physical modification by flaxseed gum and ultrasonic treatment
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Yang, Jia, Huang, Fenghong, Huang, Qingde, Ma, Da, Chen, Yashu, Peng, Dengfeng, Yu, Xiao, Deng, Qianchun, and Geng, Fang
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- 2023
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6. Effect of different structural flaxseed lignans on the stability of flaxseed oil-in-water emulsion: An interfacial perspective
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Cheng, Chen, Yu, Xiao, Huang, Fenghong, Peng, Dengfeng, Chen, Hongjian, Chen, Yashu, Huang, Qingde, and Deng, Qianchun
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- 2021
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7. Chronic alpha-linolenic acid treatment alleviates age-associated neuropathology: Roles of PERK/eIF2α signaling pathway
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Gao, Hui, Yan, Peipei, Zhang, Shun, Nie, Shuke, Huang, Fenghong, Han, Hao, Deng, Qianchun, Huang, Qingde, Yang, Wei, Wu, Hailei, Yao, Ping, Ye, Keqiang, Xu, Jiqu, and Liu, Liegang
- Published
- 2016
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8. Flaxseed lignan secoisolariciresinol diglucoside ameliorates experimental colitis induced by dextran sulphate sodium in mice
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Xu, Jiqu, Tian, Guangjing, Ma, Congcong, Gao, Hui, Chen, Chang, Yang, Wei, Deng, Qianchun, Huang, Qingde, Ma, Zhonghua, and Huang, Fenghong
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- 2016
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9. Long-Term Dietary Alpha-Linolenic Acid Supplement Alleviates Cognitive Impairment Correlate with Activating Hippocampal CREB Signaling in Natural Aging Rats
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Gao, Hui, Yan, Peipei, Zhang, Shun, Huang, Hao, Huang, Fenghong, Sun, Taoping, Deng, Qianchun, Huang, Qingde, Chen, Sijing, Ye, Keqiang, Xu, Jiqu, and Liu, Liegang
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- 2016
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10. Solid base catalysts for production of fatty acid methyl esters
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Guo, Pingmei, Zheng, Chang, Zheng, Mingming, Huang, Fenghong, Li, Wenlin, and Huang, Qingde
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- 2013
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11. Biodiesel production using magnetically stabilized fluidized bed reactor
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Guo, Pingmei, Huang, Fenghong, Huang, Qingde, and Zheng, Chang
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- 2012
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12. Exploration of suitable in vitro simulated digestion model for lipid oxidation of flaxseed oil emulsion during digestion.
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Li, Xiaowen, Huang, Qingde, Wang, Xintian, Zhang, Mingkai, Quan, Shuang, Geng, Fang, Chen, Hongjian, and Deng, Qianchun
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LINSEED oil , *BILE salts , *LIPIDS , *DIGESTION , *EMULSIONS , *SMALL intestine - Abstract
BACKGROUND: The INFOGEST model is a standardized general in vitro digestion study, but it cannot accurately simulate the fatty acid release process of lipids in the stomach and small intestine. In this study, the internationally universal INFOGEST 2019 was used as the basic model and flaxseed oil emulsion was used as the research object. In various improvement models, the effect of fatty acid release rate on the oxidation stability of flaxseed oil was assessed by adding rabbit stomach extract and changing the order of bile salts addition. RESULTS: With the presence of rabbit gastric extract, flaxseed oil emulsion flocculation and coalescence in stomach were reduced, and the absolute value of ζ‐potential increased. Moreover, the release rate of fatty acids in the small intestine increased by 12.14%. The amount of lipid oxidation product (i.e. hexanal) in the gastric and intestinal phases increased by 0.08 ppb. In addition, the fatty acid release rate in the small intestine phase increased by 5.85% and the hexanal content increased by 0.011 ppb in the digestion model of adding bile salts before adjusting the pH in the small intestine phase compared with the model of adjusting the pH first and then adding bile salts. CONCLUSION: The results obtained from this study will contribute to finding the most suitable static digestion model for simulating digestion and oxidation of lipid during lipid gastrointestinal digestion. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2022
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13. Magnetic Solid Base Catalysts for the Production of Biodiesel
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Guo, Pingmei, Huang, Fenghong, Zheng, Mingming, Li, Wenlin, and Huang, Qingde
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- 2012
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14. RETRACTED ARTICLE: Optimization of Biodiesel Production Using a Magnetically Stabilized Fluidized Bed Reactor
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Guo, Pingmei, Huang, Fenghong, Huang, Qingde, and Zheng, Chang
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- 2012
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15. Flaxseed oil and α-lipoic acid combination reduces atherosclerosis risk factors in rats fed a high-fat diet
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Xu Jiqu, Yang Wei, Deng Qianchun, Huang Qingde, Yang Jin’e, and Huang Fenghong
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Flaxseed oil ,α-lipoic acid ,Atherosclerosis ,Oxidant stress ,Plasma lipids ,Inflammation ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background Atherosclerosis is a major manifestation of the pathophysiology underlying cardiovascular disease. Flaxseed oil (FO) and α-lipoic acid (LA) have been reported to exert potential benefit to cardiovascular system. This study tried to assess the effect of supplement of FO and LA combination on the atherosclerosis risk factors in rats fed a high-fat diet. Methods LA was dissolved in flaxseed oil to a final concentration of 8 g/kg (FO+LA) when used. The rodent diet contained 20% fat. One-fifth of the fat was soybean oil and the others were lard (HFD group), or 75% lard and 25% FO+LA (L-FO+LA group), or 50% lard and 50% FO+LA (M-FO+LA group), or FO+LA (H-FO+LA group). Animals were fed for 10 weeks and then killed for blood collection. Results Supplement of FO and LA combination significantly enhanced plasma antioxidant defense capacities, as evaluated by the marked increase in the activities of SOD, CAT and GPx as well as the level of GSH, and the significant reduction in lipid peroxidation. Simultaneous intake of FO and LA also reduced plasma TG, TC and LDL-C contents and elevated the ratio of HDL-C/LDL-C. Besides, in parallel with the increase of FO and LA combination, plasma IL-6 and CRP levels were remarkably reduced. Conclusion Supplement of FO and LA combination may contribute to prevent atherogenesis by improving plasma oxidative stress, lipid profile and inflammation.
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- 2012
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16. Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level
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Yang Wei, Fu Juan, Yu Miao, Huang Qingde, Wang Di, Xu Jiqu, Deng Qianchun, Yao Ping, Huang Fenghong, and Liu Liegang
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Flaxseed oil ,Antioxidation ,Erythrocytes ,Diabetes ,Membrane ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background The erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evidence regarding the protective effects of flaxseed oil, a natural product rich in n-3 fatty acid, on lipid peroxidation, antioxidative capacity and membrane deformation of erythrocytes exposed to high glucose is limited. Methods Human peripheral blood erythrocytes were isolated and treated with 50 mM glucose to mimic hyperglycemia in the absence or presence of three different doses of flaxseed oil (50, 100 or 200 μM) in the culture medium for 24 h. The malondialdehyde (MDA) and L-glutathione (GSH) were measured by HPLC and LC/MS respectively. The phospholipids symmetry and membrane fatty acid composition of human erythrocytes were detected by flow cytometry and gas chromatograph (GC). The morphology of human erythrocyte was illuminated by ultra scanning electron microscopy. Results Flaxseed oil attenuated hyperglycemia-induced increase of MDA and decrease of GSH in human erythrocytes. Human erythrocytes treated with flaxseed oil contained higher C22:5 and C22:6 than those in the 50 mM glucose control group, indicating that flaxseed oil could reduce lipid asymmetric distribution and membrane perturbation. The ultra scanning electron microscopy and flow cytometer have also indicated that flaxseed oil could protect the membrane of human erythrocytes from deformation at high glucose level. Conclusion The flaxseed oil supplementation may prevent lipid peroxidation and membrane dysfunction of human erythrocytes in hyperglycemia.
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- 2012
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17. Laxative effects of partially defatted flaxseed meal on normal and experimental constipated mice
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Xu Jiqu, Zhou Xiaoqi, Chen Chang, Deng Qianchun, Huang Qingde, Yang Jin'e, Yang Nianhong, and Huang Fenghong
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Flaxseed ,Constipation ,Diphenoxylate ,Dietary fibers ,Laxative ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Constipation is a very common health problem in the world. Intake of sufficient amount of dietary fibers is a cornerstone in the prevention and treatment of constipation. As a traditional medicine, flaxseed has been used to treat constipation for centuries, but the controlled trials are rare. The purpose of the present study was to assess that whether partially defatted flaxseed meal (PDFM) has the potential role to facilitate fecal output in normal and experimental constipated mice. Methods After supplemented with 2.5%, 5% and 10% (w/w) PDFM (L-, M- and H -PDFM) for 14 days, the constipation models of mice were induced by atropine-diphenoxylate. The small intestinal transit rates, start time of defecation, amount of defecation and wet weight of feces were researched in normal and constipation model mice. Results M- and H-PDFM significantly increase small intestinal transit rates in constipation model mice. All dose of PDFM markedly shortened the start time of defecation and M- and H-PDFM significantly increase stool frequency and weight in both normal and constipation model mice. Conclusions PDFM may be a useful laxative to facilitate fecal output in normal and constipation conditions.
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- 2012
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18. Rapeseed oil fortified with micronutrients reduces atherosclerosis risk factors in rats fed a high-fat diet
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Yang Jin'e, Huang Qingde, Deng Qianchun, Zhou Xiaoqi, Xu Jiqu, and Huang Fenghong
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Rapeseed oil ,Polyphenols ,Tocopherols ,Phytosterols ,Atherosclerosis ,Oxidant stress ,Plasma lipids ,Inflammation ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to cardiovascular system, but most of these micronutrients are removed by the refining process. The aim of this study was to determine the effect of rapeseed oil fortified with these micronutrients on the atherosclerosis risk factors in rats fed a high-fat diet. Methods The rodent diet contained 20% fat whose source was refined rapeseed oil (RRO) or fortified refined rapeseed oil with low, middle and high quantities of these micronutrients (L-, M- and H-FRRO). Forty male SD rats were divided into four groups. One group received RRO diet and other groups received L-, M- and H-FRRO diet for 10 weeks. Results Micronutrients supplementation significantly increased plasma antioxidant defense capacities, as evaluated by the significant elevation in the activities of GPx, CAT and SOD as well as the level of GSH, and the significant decline in lipid peroxidation. These micronutrients also reduced the plasma contents of TG, TC and LDL-C and increased the ratio of HDL-C/LDL-C. In addition, in parallel with the enhancement of these micronutrients, plasma levels of IL-6 and CRP declined remarkably. Conclusion Rapeseed oil fortified with micronutrients polyphenols, tocopherols and phytosterols may contribute to prevent atherogenesis by ameliorating plasma oxidative stress, lipid profile and inflammation.
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- 2011
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19. Flaxseed oligosaccharides alleviate DSS-induced colitis through modulation of gut microbiota and repair of the intestinal barrier in mice.
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Xu, Zhenxia, Chen, Wenchao, Deng, Qianchun, Huang, Qingde, Wang, Xu, Yang, Chen, and Huang, Fenghong
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- 2020
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20. Preparation of rapeseed oil with superhigh canolol content and superior quality characteristics by steam explosion pretreatment technology.
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Yu, Gaiwen, Guo, Tingting, and Huang, Qingde
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RAPESEED oil ,EXPLOSIONS ,TECHNOLOGY ,DECARBOXYLATION - Abstract
In this study, rapeseed was pretreated by steam explosion pretreatment technology and subsequently pressed to prepare rapeseed oil. GC, UPLC, and HPLC techniques were employed to analyze the quality characteristics of the rapeseed oil, including the canolol content and other quality characteristics. Additionally, the effect of steam explosion pretreatment technology on the canolol content of rapeseed oil was studied and the formation mechanism of canolol elucidated. The results revealed that when the steam explosion pressure reached 1.0 MPa, the canolol content of the tested oil increased from 41.21 to 2,168.69 mg/kg (52.63‐fold increase) and that sinapic acid played a significant role in the conversion of canolol. Thus, the sinapine was converted into the intermediate (sinapic acid) by hydrolysis, which in turn was transformed into canolol through decarboxylation. The instantaneous high‐energy environment generated by steam explosion pretreatment could intensify the hydrolysis and decarboxylation reactions of sinapine and sinapinic acid, thereby significantly increasing the canolol content of the oil. To prove the superiority of steam explosion pretreatment, we compared it with other pretreatment technologies, including traditional high‐temperature roasting and popular microwave pretreatment. The results revealed that rapeseed oil prepared by steam explosion pretreatment displayed the best quality characteristics. This study can be a reference for the preparation process of rapeseed oil with superhigh canolol content and superior quality characteristics using steam explosion pretreatment. [ABSTRACT FROM AUTHOR]
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- 2020
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21. Preparation of high‐quality concentrated fragrance flaxseed oil by steam explosion pretreatment technology.
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Yu, Gaiwen, Guo, Tingting, Huang, Qingde, Shi, Xunwang, and Zhou, Xin
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LINSEED oil ,ODORS ,EXPLOSIONS ,FOOD safety ,TECHNOLOGY ,DETONATION waves ,FATTY acids - Abstract
In this study, flaxseed was pretreated by steam explosion technology and subsequently pressed to prepare flaxseed oil. GC, UPLC, HPLC, and GC‐MS techniques were used to analyze the quality characteristics of the prepared flaxseed oil. These included the food safety risk indices, micronutrient components, and oxidative stability. The effects of different steam explosion pressures on the quality characteristics and relative volatile components of flaxseed oil were also investigated. The results revealed that steam explosion pretreatment technology could significantly increase the oil yield, improve micronutrient content, and strengthen the oxidation stability of the product. Moreover, the food safety risk indices (e.g., benzopyrene) were controlled within a reasonable range, while the fatty acid content remained almost unchanged. Notably, the relative pyrazine content in the total volatile components of flaxseed oil was 68.25% when the steam explosion pressure reached 1.2 MPa. This was considered as the main factor that contributed to the unique concentrated fragrance of the produced flaxseed oil. To prove the superiority of the steam explosion pretreatment, we compared this technique with traditional high‐temperature roasting and popular microwave pretreatment techniques. The results revealed that flaxseed oil prepared by steam explosion pretreatment displayed the best quality characteristics and most concentrated fragrance. Thus, steam explosion technology shows great potential for application to produce high‐quality concentrated fragrance flaxseed oil. This study provides significant reference and guidance for the preparation process of flaxseed oil. [ABSTRACT FROM AUTHOR]
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- 2020
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22. Retraction Note: Optimization of Biodiesel Production Using a Magnetically Stabilized Fluidized Bed Reactor
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Guo, Pingmei, Huang, Fenghong, Huang, Qingde, and Zheng, Chang
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- 2013
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23. Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice.
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Xu, Jiqu, Xia, Zumeng, Rong, Shuang, Gao, Hui, Yang, Wei, Li, Jieliang, Ma, Congcong, Deng, Qianchun, Huang, Qingde, Xiao, Lingyun, and Huang, Fenghong
- Abstract
Atherosclerosis (AS) is the main cause of cardiovascular diseases. This study investigated Yirui (YR) capsules, whose ingredients are available in health food stores, against AS and the underlying mechanisms. Male apolipoprotein E-deficient mice fed a high-fat diet for 10 weeks developed severe aortic lesions, but YR significantly decreased the plaque area in the total aorta and aortic root. YR affected the serum lipid profile by significantly reducing total cholesterol, low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), and oxidative modification of LDL-C (Ox-LDL) levels. In addition, multi-cytokine analysis revealed that higher serum levels of interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-3 (IL-3), interleukin-6 (IL-6), interleukin-27 (IL-27), tumor necrosis factor alpha, interferon gamma, and regulated on activation, normal T cell expressed and secreted (RANTES), which were induced by a high-fat diet, declined with YR treatment. These results suggest that YR reduces the atherosclerotic plaque burden, thereby alleviating AS by modulating the lipid profile and inhibiting inflammation. [ABSTRACT FROM AUTHOR]
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- 2018
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24. Comparative analysis of the <italic>in-vitro</italic> antioxidant activity and bioactive compounds of flaxseed in China according to variety and geographical origin.
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Deng, Qianchun, Yu, Xiao, Ma, Fangli, Xu, Jiqu, Huang, Fenghong, Huang, Qingde, and Sheng, Feng
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FLAXSEED ,BIOACTIVE compounds ,OXIDANT status ,PHENOLIC acids ,PHYTOSTEROLS - Abstract
The present study aimed to use a chemometric study to compare the
in-vitro antioxidant activities and bioactive compounds of flaxseed based on the variety and geographical origins, and further explore the specific components constructed the endogenous antioxidant system of flaxseed in China. The differential contents of α-linolenic acid (35.83-58.91%), lignans (11.37-33.31 mg/g), total phenolic acids (109.93-246.88 mg/100 g) and phytosterols (56.52-125.12 mg/g) of 32 flaxseed varieties were observed. Concomitantly, thein-vitro antioxidant activities varied from 32.56 to 46.22 mg TE/100 g, 0.58 to 1.08 mg TE/g, and 14.22 to 36.14 mmol TE/g as determined by the 2,2’-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing antioxidant power (FRAP) and 2,2′-azinobis-(3-ethylbenzthiazoline 6-sulfonic acid) (ABTS) assays, respectively. Significant correlations were found between the antioxidant activities and contents of free syringic acid,p -coumaric acid glucoside and secoisolariciresinol diglucoside (SDG) (p < 0.01). Principal component analysis showed a relatively dispersed distribution between samples, which was not effective enough to determine the geographical origins of the flaxseed samples. Hierarchical cluster analysis allowed flaxseed samples to be grouped based on theirin-vitro antioxidant activities and bioactive component contents, primarily in relation to variety specificity. [ABSTRACT FROM AUTHOR]- Published
- 2017
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25. Retraction notice to biodiesel production using magnetically stabilized fluidized bed reactor
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Guo, Pingmei, Huang, Fenghong, Huang, Qingde, and Zheng, Chang
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- 2013
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26. Aqueous Enzymatic Extraction of Rapeseed Oil and Protein from Dehulled Cold-pressed Double-low Rapeseed Cake.
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Niu, Yan Xing, Li, Wenlin, Zhu, Jun, Huang, Qingde, Jiang, Mulan, and Huang, Fenghong
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RAPESEED oil ,ENZYMATIC analysis ,PROTEIN content of food ,CAKE ,EXTRACTION (Chemistry) ,TRANSMISSION electron microscopes ,RESPONSE surfaces (Statistics) - Abstract
The oil and protein of dehulled cold-pressed double-low rapeseed cake was extracted by an aqueous enzymatic process. The rapeseed cake was treated by the chosen combined enzymes of Viscozyme L and Alcalase 2.4L (VLA,1:1,w/w). Preliminary experiments and Response Surface Methodology (RSM) were used to study the effects of enzyme concentration, incubation time and water-to-cake ratio on the extraction yield of oil and protein. This is how the desirable conditions were obtained. Transmissive electron microscope photo showed that after cold-pressing the cell structure of rapeseed was partly damaged while dehulling had little effect on the cell structure of rapeseed. In RSM experiments water-to-cake ratio showed significant effects on the extraction of oil and protein (P<0.05),while incubation time only showed significant effects on protein yield (P<0.05).The desirable conditions were as follows: 1.0% concentration (w/w) of VLA; water-to-cake ratio(w/w),6:1; 80 min incubation time. Under this condition, the extraction yield of protein and oil were 82.10% and 71.89%, respectively. Through combining both the cold-press and the aqueous enzymatic processes together, the total oil yield reached 91.6%, which is higher than the normal cold-press process or the aqueous enzymatic extraction process alone. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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27. Effect of Flaxseed Oil Fortified with Vitamin E and Phytosterols on Antioxidant Defense Capacities and Lipids Profile in Rats.
- Author
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Deng, Qianchun, Yu, Xiao, Xu, Jiqu, Liu, Changsheng, Huang, Fenghong, Huang, Qingde, and Yang, Jine
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LINSEED oil ,ENRICHED foods ,VITAMIN E content of food ,PHYTOSTEROLS ,BIOLOGICAL assay of antioxidants ,MEASUREMENT of lipids in the body ,MICRONUTRIENTS ,CARDIOVASCULAR diseases -- Nutritional aspects - Abstract
The main proposal of this study was to evaluate in vivo whether flaxseed oils fortified with micronutrients would have beneficial effects on lipid profile and antioxidant status in high-fat fed rats. Male Wistar rats were fed synthetic diets containing 10% of flaxseed oil (FO), phytosterols (PS) fortified FO (PS-FO), vitamin E (V
E ) fortified FO (VE -FO), VE , and PS fortified FO (VE -PS-FO), respectively, for 4 wk. The results showed that VE fortified FO enhanced the enzymatic (SOD and GPX) and nonenzymatic (GSH and VE ) antioxidant system, lowered the lipid peroxide (TBARS) concentration compared with FO ( P < 0.05). And FO fortified with PS significantly reduced the plasma TG, TC and LDL-C levels, and hepatic TG and TC levels of rats compared with FO ( P < 0.05), but had no significant effect on antioxidant defense capacities. Combined addition of VE and PS in FO had a synergetic effect. These results indicated that flaxseed oils fortified micronutrients VE and PS may contribute to reduce the risk factors of cardiovascular disease (CVD) by improving plasma antioxidant defenses and lipids profiles. Practical Application: Flaxseed oil usually contains greater than 50% of alpha-linolenic acid (ALA) and is a desire origin for n-3 PUFA. But consuming high dose of n-3 PUFA could lead to oxidative damage through free radical-chain reaction in cellular and subcellular membranes. Our studies showed that a regular intake of VE and PS fortified flaxseed oils increased antioxidant defenses and ameliorated lipids profile in high-fat fed rats, and these indicated that the flaxseed oil fortified with these micronutrients might reduce the incidence of CVD. [ABSTRACT FROM AUTHOR]- Published
- 2012
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28. Optimization of Biodiesel Production Using a Magnetically Stabilized Fluidized Bed Reactor.
- Author
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Guo, Pingmei, Huang, Fenghong, Huang, Qingde, and Zheng, Chang
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BIODIESEL fuels ,COTTONSEED oil ,TRANSESTERIFICATION ,METHANOL ,FATTY acids - Abstract
A biodiesel production process using magnetically stabilized fluidized bed reactor (MSFBR) has been developed based on the refined cottonseed oil. The reactant flow rate and magnetic field intensity effects on the nanometer magnetic catalyst behavior in the column were investigated. Orthogonal experiments (L(2)) were applied to optimize the best transesterification reaction conditions. Reaction temperature, methanol to oil molar ratio, and reactant flow rate were the main factors to influence transesterification conversion efficiency. These three factors chosen for the present investigation were based on the results of single-factor tests. The optimum transesterification reaction conditions of cottonseed oil were determined in MSFBR as follows: methanol to oil molar ratio 8:1, 40 cm min reactant flow rate, 225 Oe magnetic field intensity and reaction temperature of 65 °C, the conversion efficiency reached 97% in 100 min. The cold filter plugging point and kinematic viscosity of cottonseed oil biodiesel were higher than that described by Chinese specifications of biodiesel because of the special fatty acid profiles of cottonseed oil. The activity stability of the nanometer magnetic solid catalyst in MSFBR was much better than that in the autoclave stirred reactor (ASR). [ABSTRACT FROM AUTHOR]
- Published
- 2012
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29. In Vitro Digestion and Fermentation by Human Fecal Microbiota of Polysaccharides from Flaxseed.
- Author
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Zhou, Xin, Zhang, Zhao, Huang, Fenghong, Yang, Chen, and Huang, Qingde
- Subjects
HUMAN microbiota ,DIGESTION ,POLYSACCHARIDES ,SHORT-chain fatty acids ,BUTYRIC acid ,GUT microbiome ,FERMENTATION ,FLAXSEED - Abstract
The digestion of flaxseed polysaccharides (FSP) in simulated saliva, gastric and small intestine conditions was assessed, as well as in vitro fermentation of FSP by human gut microbiota. FSP was not degraded in the simulated digestive systems (there was no change in molecular weight or content of reducing sugars), indicating that ingested FSP would reach the large intestine intact. Changes in carbohydrate content, reducing sugars and culture pH suggested that FSP could be broken down and used by gut microbiota. FSP modulated the composition and structure of the gut microbiota by altering the Firmicutes/Bacteroidetes ratio and increasing the relative abundances of Prevotella, Phascolarctobacterium, Clostridium and Megamonas, which can degrade polysaccharides. Meanwhile, FSP fermentation increased the concentration of short-chain fatty acids, especially propionic and butyric acids. Our results indicate that FSP might be developed as a functional food that benefits gut health. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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30. Melatonin alleviates cognition impairment by antagonizing brain insulin resistance in aged rats fed a high‐fat diet.
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Xu, Jiqu, Gao, Hui, Zhang, Li, Rong, Shuang, Yang, Wei, Ma, Congcong, Chen, Meng, Huang, Qingde, Deng, Qianchun, and Huang, Fenghong
- Subjects
INSULIN resistance ,HIGH-fat diet ,BRAIN - Abstract
Brain insulin resistance, induced by neuroinflammation and oxidative stress, contributes to neurodegeneration, that is, processes that are associated with Aβ accumulation and TAU hyperphosphorylation. Here, we tested the effect of chronic administration of melatonin (MLT) on brain insulin resistance and cognition deficits caused by a high‐fat diet (HFD) in aged rats. Results showed that MLT supplementation attenuated peripheral insulin resistance and lowered hippocampal oxidative stress levels. Activated microglia and astrocytes and hippocampal levels of TNF‐α in HFD‐fed rats were reduced by MLT treatment. Melatonin also prevented HFD‐induced increases in beta‐amyloid (Aβ) accumulation and TAU phosphorylation in the hippocampus. In addition, impairments of brain insulin signaling elicited by long‐term HFD were restored by MLT treatment, as confirmed by ex vivo insulin stimulation. Importantly, MLT reversed HFD‐induced cognitive decline as measured by a water maze test, normalized hippocampal LTP and restored CREB activity and BDNF levels as well as cholinergic neuronal activity in the hippocampus. Collectively, these findings indicate that MLT may exhibit substantial protective effects on cognition, via restoration of brain insulin signaling. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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31. A facile and efficient strategy for the fabrication of porous linseed gum/cellulose superabsorbent hydrogels for water conservation.
- Author
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Zhang, Hao, Luan, Qian, Huang, Qingde, Tang, Hu, Huang, Fenghong, Li, Wenlin, Wan, Chuyun, Liu, Changsheng, Xu, Jiqu, Guo, Pingmei, and Zhou, Qi
- Subjects
- *
FLAXSEED , *HYDROGELS , *WATER conservation , *FABRICATION (Manufacturing) , *SUPERABSORBENT polymers - Abstract
The linseed gum/cellulose composite hydrogels were successfully fabricated by mixing cellulose and linseed gum solutions dissolved in the NaOH/urea aqueous system and cross-linked with epichlorohydrin. The morphology and structure of the composite hydrogels were investigated by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffractometry (XRD) and thermogravimetric analysis (TGA). The swelling ratio and water retention properties were investigated. The results revealed that linseed gum mainly contributed to water adsorption, whereas the cellulose acted as a backbone to strengthen the porous structure. This work provided a simple way to prepare cellulose-based superabsorbent hydrogels, which could be potentially applied as an effective water conservation material in agriculture. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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32. Optimized endogenous lipid concomitants in flaxseed oil by different oil extraction technologies: Their positive roles in emulsions.
- Author
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Cheng, Chen, Yu, Kun, Yu, Xiao, Geng, Fang, Huang, Fenghong, Wang, Lei, Huang, Qingde, Quan, Shuang, and Deng, Qianchun
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LINSEED oil , *EMULSIONS , *LIPIDS , *EDIBLE fats & oils , *PHOSPHOLIPIDS , *SOLVENT extraction - Abstract
In this study, different oil extraction technologies were used to obtain flaxseed oil with different lipid concomitants, and the influences of endogenous lipid concomitants on the stability and digestion properties of flaxseed oil emulsions were investigated. Compared with cold-press (CP), microwave pretreatment coupled with cold-press (MPCP) and accelerated solvent extraction (ASE) contributed to the accumulation of hydrophilic and lipophilic lipid concomitants, respectively. Subcritical fluid extraction (SFE) and supercritical CO 2 extraction (SCO 2 E) were conducive to the accumulation of amphipathic lipid concomitants. After emulsification, ASE-flaxseed oil emulsions had the lowest initial particle size due to the high content of amphiphilic lipid concomitants phospholipids. Phospholipids are spontaneously adsorbed on the interface of flaxseed oil and water. Antioxidative lipid concomitants (phenols, phytosterols, carotenoids, flavonoids, and cyclolinopeptides (CLs)) in MPCP-flaxseed oil improved the oxidative stability of emulsions. After digestion, ASE-flaxseed oil emulsion presented the highest FFAs% (∼78%) owing to smaller particle size. The release of FFAs decreased by 5%–10% in MPCP-, SCO 2 E-, and SFE-flaxseed oil emulsions ascribed to the high content of phenols. In conclusion, MPCP and ASE are powerful flaxseed oil extraction technologies for improving the stability and digestion of emulsions, respectively. [Display omitted] • The methods of CP, MPCP, ASE, SCO 2 E and ASE were used to extract flaxseed oil. • Oil extraction methods influenced the content of lipid concomitants in flaxseed oil. • SCO 2 E-flaxseed oil emulsions were unstable for the prooxidative activity of FFAs. • MPCP-flaxseed oil emulsion was stable for high content of tocopherols, flavonoids. • ASE-flaxseed oil emulsions promoted FFAs release for its lower diameter size. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
33. Functional properties of protein isolates, globulin and albumin extracted from Ginkgo biloba seeds
- Author
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Deng, Qianchun, Wang, Lan, Wei, Fang, Xie, Bijun, Huang, FengHong, Huang, Wen, Shi, John, Huang, Qingde, Tian, Binqiang, and Xue, Sophia
- Subjects
- *
GLOBULINS , *ALBUMINS , *GINKGO , *SEED proteins , *ABSORPTION of water in plants , *SOLUBILITY - Abstract
Abstract: In this study, the functional properties of Ginkgo seed protein isolate (GPI), Ginkgo seed globulin protein (GGP) and Ginkgo seed albumin protein (GAP) extracted from Ginkgo biloba seeds were investigated. The protein contents of GPI, GGP and GAP were 91.0%, 93.4% and 87.8%, respectively in the samples in which the sugar, polyphenol and crude fibre were removed by the preparation procedure. For functional properties of Ginkgo seed proteins in the natural state, GAP showed the highest oil-absorption capacity (9.3ml/g), foaming capacity (67.8%), emulsifying capacity (65.4%) and emulsion stability (90.6%); while GPI showed the highest water absorption capacity (1.93ml/g), and GGP showed the highest foam stability (55.5%). The differences of the chemical components, surface hydrophobicity, disulphide bond (SS) and sulfhydryl group (SH) contents of GPI, GGP and GAP, which were correlated significantly with functional properties of Ginkgo seed proteins, were also investigated. The improved functional properties, such as water absorption capacity, solubility, foaming properties and emulsifying properties of Ginkgo seed proteins were observed in a pH range of 8.0–10.0 or sodium chloride concentration of 0.5–0.75M. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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34. Purification and characterization of an antioxidant protein from Ginkgo biloba seeds
- Author
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Huang, Wen, Deng, Qianchun, Xie, Bijun, Shi, John, Huang, FengHong, Tian, Binqiang, Huang, Qingde, and Xue, Sophia
- Subjects
- *
ANTIOXIDANTS , *CHEMICAL purification , *GINKGO , *CHEMILUMINESCENCE , *MOLECULAR weights , *POLYSACCHARIDES , *DRUG activation - Abstract
Abstract: A new antioxidation protein named G4b was purified from Ginkgo biloba seed albumin. The results of chemical colorimetry and chemiluminescence methods showed that the hemi-inhibitory concentration (IC50) of G4b on the hydroxyl radical were 78.1μg/mL and 115.1μg/mL, and the IC50 on DPPH was 100.7μg/mL, while the IC50 on the superoxide anion and DNA injury were 23μg/mL and 27μg/mL, respectively, and the abundance of sulfur amino acids and aromatic amino acids contained in G4b might contribute to its strong antioxidation properties. G4b is a kind of homogeneous and novel protein with a molecular mass of 29,247Da, consisting of two peptides with similar molecular weights linked by a disulfide bond. Result of β-elimination reaction showed that G4b is connected with a small quantity of polysaccharide by an oxygen-glucosidic bond. [Copyright &y& Elsevier]
- Published
- 2010
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35. Beneficial effects of flaxseed polysaccharides on metabolic syndrome via gut microbiota in high-fat diet fed mice.
- Author
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Yang, Chen, Xu, Zhenxia, Deng, Qianchun, Huang, Qingde, Wang, Xu, and Huang, Fenghong
- Subjects
- *
GUT microbiome , *METABOLIC syndrome , *HIGH-fat diet , *LIPID metabolism disorders , *FLAX - Abstract
• Flaxseed polysaccharide supplementation ameliorated the metabolic syndrome in HFD mice. • Flaxseed polysaccharide supplementation increased the proportion of Akkermansia and Bifidobacterium. • The improvement of lipid metabolism disorder was associated with the modulation of the gut microbiota. Flaxseed (Linum usitatissimum L.) is known as healthy food for its anti-obesity and lipid modulating properties. However, the effects of flaxseed polysaccharide (FSP) on metabolic syndrome (MetS) and gut microbiota are still poorly understood. Here, we investigated the effects of FSP on lipid metabolism and gut microbiota in high-fat-diet-fed mice. FSP effectively reduced the serum fasting glucose, total triglyceride and total cholesterol levels. FSP consumption adipose accumulation impacted the gut microbiome at different taxonomic levels by increasing the proportions of beneficial Akkermansia and Bifidobacterium and decreasing the disease or obesity associated Oscillospira and Odoribacteraceae. These changes were highly correlated with the regulation of expression levels of lipid metabolism involved genes in the liver. The restoration of total SCFAs, especially propionate and butyrate might be an important strategy for mitigating HFD induced metabolic disorders. These findings suggest that FSP may use as a prebiotic for preventing MetS by modulating the gut microbiota. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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36. Effect of flaxseed polyphenols on physical stability and oxidative stability of flaxseed oil-in-water nanoemulsions.
- Author
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Cheng, Chen, Yu, Xiao, McClements, David Julian, Huang, Qingde, Tang, Hu, Yu, Kun, Xiang, Xia, Chen, Peng, Wang, Xintian, and Deng, Qianchun
- Subjects
- *
FLAXSEED , *LINSEED oil , *POLYPHENOLS , *FUNCTIONAL beverages , *FREE radicals , *FUNCTIONAL foods , *PLANT polyphenols - Abstract
• Flaxseed polyphenols exerted differential antioxidant effects in nanoemulsion. • SECO and flaxseed lignan macromolecule inhibited lipid oxidation in nanoemulsion. • The mechanism mainly related to the partitioning of polyphenols in nanoemulsion. • The glucoside in phenolic extracts decreased their association with lipid droplets. Recent studies have shown that the high susceptibility of flaxseed oil nanoemulsions to lipid oxidation limits their incorporation into functional foods and beverages. For this reason, the impact of various flaxseed phenolic extracts on the physical and oxidative stability of flaxseed oil nanoemulsions was investigated. Flaxseed lignan extract (FLE) and secoisolariciresinol (SECO) exhibited antioxidant activity whereas secoisolariciresinol diglucoside (SDG) and p -coumaric acid (CouA) exhibited prooxidant activity in the flaxseed oil nanoemulsions. The antioxidant potential of flaxseed phenolics in the nanoemulsions was as follows: SECO < CouA < SDG ≈ FLE. Moreover, the antioxidant/prooxidant activity of the phenolics was also related to their free radical scavenging activity and partitioning in the nanoemulsions. Our results suggested that both SECO and FLE were good plant-based antioxidants for improving the stability of flaxseed oil nanoemulsions. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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37. A Combination of Flaxseed Oil and Astaxanthin Improves Hepatic Lipid Accumulation and Reduces Oxidative Stress in High Fat-Diet Fed Rats.
- Author
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Xu J, Rong S, Gao H, Chen C, Yang W, Deng Q, Huang Q, Xiao L, and Huang F
- Subjects
- Acetyl-CoA Carboxylase genetics, Acetyl-CoA Carboxylase metabolism, Animals, Cholesterol metabolism, Diet, High-Fat, Dietary Fats, Fatty Acid Synthases genetics, Fatty Acid Synthases metabolism, Lipid Peroxidation drug effects, Liver metabolism, Male, Non-alcoholic Fatty Liver Disease physiopathology, Oxidoreductases genetics, Oxidoreductases metabolism, PPAR gamma genetics, PPAR gamma metabolism, Rats, Rats, Sprague-Dawley, Sterol Regulatory Element Binding Protein 1 genetics, Sterol Regulatory Element Binding Protein 1 metabolism, Triglycerides blood, Xanthophylls administration & dosage, Linseed Oil administration & dosage, Liver drug effects, Non-alcoholic Fatty Liver Disease diet therapy, Oxidative Stress drug effects
- Abstract
Hepatic lipid accumulation and oxidative stress are crucial pathophysiological mechanisms for non-alcoholic fatty liver disease (NAFLD). Thus, we examined the effect of a combination of flaxseed oil (FO) and astaxanthin (ASX) on hepatic lipid accumulation and oxidative stress in rats fed a high-fat diet. ASX was dissolved in flaxseed oil (1 g/kg; FO + ASX). Animals were fed diets containing 20% fat, where the source was lard, or 75% lard and 25% FO + ASX, or 50% lard and 50% FO + ASX, or FO + ASX, for 10 weeks. Substitution of lard with FO + ASX reduced steatosis and reduced hepatic triacylglycerol and cholesterol. The combination of FO and ASX significantly decreased hepatic sterol regulatory element-binding transcription factor 1 and 3-hydroxy-3-methylglutaryl-CoA reductase but increased peroxisome proliferator activated receptor expression. FO + ASX significantly suppressed fatty acid synthase and acetyl CoA carboxylase but induced carnitine palmitoyl transferase-1 and acyl CoA oxidase expression. FO + ASX also significantly elevated hepatic SOD, CAT and GPx activity and GSH, and markedly reduced hepatic lipid peroxidation. Thus, FO and ASX may reduce NAFLD by reversing hepatic steatosis and reducing lipid accumulation and oxidative stress.
- Published
- 2017
- Full Text
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38. Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet.
- Author
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Deng Q, Yu X, Xu J, Kou X, Zheng M, Huang F, Huang Q, and Wang L
- Subjects
- Animals, Cholesterol blood, Cholesterol, LDL blood, Cricetinae, Male, Mesocricetus, Risk Factors, Triglycerides blood, Atherosclerosis blood, Atherosclerosis prevention & control, Diet, High-Fat adverse effects, alpha-Linolenic Acid therapeutic use
- Abstract
Background: Emerging evidence suggested phytosterol esters (PE) exhibited an advantage over naturally occurring phytosterols in reducing atherosclerosis risk factors due to improved fat solubility and compatibility. However, the effects of dietary patterns of PE on lipid-lowering activity were limited and inconsistent. This study aimed to explore the effects of dose and frequency of α-linolenic acid rich phytosterol esters (ALA-PE) on cholesterol and triglyceride metabolism markers focused on intestinal cholesterol absorption and bioconversion of ALA in liver., Methods: Dose-dependency study Male Syrian golden hamsters were fed high-fat diets (HFD) containing low, medium and high dose of ALA-PE (0.72 %, 2.13 % and 6.39 %) for 6 weeks. The high fat diet contained 89.5 % chow diet, 0.2 % cholesterol, 10 % lard and 0.3 % bile salt. Dose-frequency study Male Syrian golden hamsters were provided: (I) 0.4 mL/100 g peanut oil by gavage once a day; (II) 0.4 mL/100 g ALA-PE by gavage once a day; (III) 0.2 mL/100 g ALA-PE by gavage twice a day; (IV) 0.133 mL/100 g ALA-PE by gavage three times a day; (V) 0.1 mL/100 g ALA-PE by gavage four times a day for 6 weeks with a high-fat diet simultaneously., Results: ALA-PE dose-dependently lowered plasma total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) concentrations with a maximal decrease of 42 %, 59 % and 73 %, respectively (p < 0.05). Compared to HFD, TC, LDL-C and TG concentrations were significantly lower (p < 0.01) in hamsters consumed HFD plus ALA-PE for 1-4 times per day but there were not remarkable differences among different consumption frequencies. No significant changes in plasma antioxidant capacity and lipid peroxidation levels were observed among HFD and HFD plus different doses of ALA-PE groups. The contents of hepatic α-linolenic (ALA), docosapentaenoic (DPA) and docosahexaenoic (DHA) acids were dose-dependently increased in different ALA-PE groups compared to those in HFD group. The abundance of mRNA for intestinal sterol transporters Niemann-Pick C1-Like 1 (NPC1L1), ATP-binding cassette (ABC) transporters ABCG5 and ABCG8 indicated no significant differences among all groups., Conclusion: ALA-PE dose-dependently improved lipid profile in hamsters fed HFD independent of intestinal ABCG5, ABCG8 and NPC1L1, accompanying by increased conversion of ALA to DPA and DHA in liver. ALA-PE manifested "once a day" lipid-lowering efficacy, highlighting a promising preventive strategy for metabolic syndrome.
- Published
- 2016
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39. Optimized Rapeseed Oils Rich in Endogenous Micronutrients Protect High Fat Diet Fed Rats from Hepatic Lipid Accumulation and Oxidative Stress.
- Author
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Xu J, Liu X, Gao H, Chen C, Deng Q, Huang Q, Ma Z, and Huang F
- Subjects
- Animals, Cholesterol analysis, Fatty Acids, Monounsaturated, Food Handling methods, Liver chemistry, Male, Non-alcoholic Fatty Liver Disease prevention & control, PPAR alpha analysis, Plant Oils chemistry, Rapeseed Oil, Rats, Rats, Wistar, Sterol Regulatory Element Binding Protein 1 analysis, Triglycerides analysis, Diet, High-Fat adverse effects, Lipid Metabolism drug effects, Liver metabolism, Micronutrients administration & dosage, Oxidative Stress drug effects, Plant Oils therapeutic use
- Abstract
Unlabelled: Micronutrients in rapeseed exert a potential benefit to hepatoprotection, but most of them are lost during the conventional refining processing. Thus some processing technologies have been optimized to improve micronutrient retention in oil. The aim of this study is to assess whether optimized rapeseed oils (OROs) have positive effects on hepatic lipid accumulation and oxidative stress induced by a high-fat diet., Methods: Rats received experiment diets containing 20% fat and refined rapeseed oil or OROs obtained with various processing technologies as lipid source. After 10 weeks of treatment, liver was assayed for lipid accumulation and oxidative stress., Results: All OROs reduced hepatic triglyceride contents. Microwave pretreatment-cold pressing oil (MPCPO) which had the highest micronutrients contents also reduced hepatic cholesterol level. MPCPO significantly decreased hepatic sterol regulatory element-binding transcription factor 1 (SREBP1) but increased peroxisome proliferator activated receptor α (PPARα) expressions, and as a result, MPCPO significantly suppressed acetyl CoA carboxylase and induced carnitine palmitoyl transferase-1 and acyl CoA oxidase expression. Hepatic catalase (CAT) and glutathione peroxidase (GPx) activities as well as reduced glutathione (GSH) contents remarkably increased and lipid peroxidation levels decreased in parallel with the increase of micronutrients., Conclusion: OROs had the ability to reduce excessive hepatic fat accumulation and oxidative stress, which indicated that OROs might contribute to ameliorating nonalcoholic fatty liver induced by high-fat diet.
- Published
- 2015
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40. Effects of endogenous and exogenous micronutrients in rapeseed oils on the antioxidant status and lipid profile in high-fat fed rats.
- Author
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Deng Q, Yu X, Xu J, Wang L, Huang F, Huang Q, Liu C, and Ma F
- Subjects
- Animals, Cardiovascular Diseases etiology, Cardiovascular Diseases prevention & control, Diet, High-Fat adverse effects, Drug Evaluation, Preclinical, Fatty Acids, Monounsaturated, Lipid Metabolism, Lipid Peroxidation, Lipids blood, Male, Micronutrients, Oxidative Stress, Phytosterols metabolism, Rapeseed Oil, Rats, Wistar, Antioxidants administration & dosage, Plant Oils administration & dosage
- Abstract
Background: Micronutrients in oil reduce one or more risk factors of cardiovascular diseases, while the contents of micronutrients in oil are relatively poor, which is insufficient to reverse the metabolic disorders at different stages of progress. The aim of this study was to investigate the effects of endogenous micronutrients in optimized cold-pressed rapeseed oil and restoratively added or fortified micronutrients in traditional refined rapeseed oil (restoring micronutrients to be nearly equal to or significantly higher than levels in crude rapeseed oil) on the antioxidant status and lipid profile in high-fat fed rats., Methods: Male Wistar rats were fed high-fat diets containing different rapeseed oils for 4 weeks, including the standard refined rapeseed oil(SRO), optimized cold-pressed rapeseed oil(CRO) and the traditional refined rapeseed oil with restorative addition or fortification of micronutrients (LF, HF-SRO)., Results: CRO exhibited significant increases in contents of tocopherols (+13%), phytosterols (+34%), polyphenols (+92%) and phospholipids (+725%) compared with SRO, as well as the total antioxidant capacities (+82-125%) (p<0.05). While the HF-SRO revealed improved antioxidant properties in vitro than the CRO, which was comparable to LF-SRO. Significant improved plasma antioxidant capacities and lipid peroxidation evaluated by T-AOC, GSH, tocopherols and MDA were found in rats fed HF-SRO when compared with CRO and LF-SRO (p<0.05). Furthermore, HF-SRO also decreased the plasma and hepatic TC levels compared to CRO and LF-SRO, accompanying higher fecal cholesterol excretion (p<0.05)., Conclusion: The standard refined rapeseed oil with fortification, not restorative addition of micronutrients was comparable to the optimized cold-pressed rapeseed oil in improving the antioxidant status and lipid profile of high-fat fed rats.
- Published
- 2014
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- View/download PDF
41. Optimized rapeseed oils rich in endogenous micronutrients ameliorate risk factors of atherosclerosis in high fat diet fed rats.
- Author
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Xu J, Ma C, Han L, Gao H, Zhou Q, Yang M, Chen C, Deng Q, Huang Q, and Huang F
- Subjects
- Animals, Atherosclerosis blood, Atherosclerosis etiology, C-Reactive Protein metabolism, Diet, High-Fat adverse effects, Drug Evaluation, Preclinical, Fatty Acids, Monounsaturated, Interleukin-6 blood, Lipid Peroxidation, Lipids blood, Male, Micronutrients, Plant Extracts therapeutic use, Plant Oils therapeutic use, Rapeseed Oil, Rats, Wistar, Risk Factors, Atherosclerosis drug therapy, Brassica rapa chemistry, Plant Extracts pharmacology, Plant Oils pharmacology
- Abstract
Background: Micronutrients in rapeseed such as polyphenols, tocopherols, phytosterols and phospholipids in rapeseed exert potential benefit to atherosclerosis. Some part of these healthy components substantially lost during the conventional refining processing. Thus some new processing technologies have been developed to produce various endogenous micronutrient-enriched optimized rapeseed oils. The aim of this study is to assess whether optimized rapeseed oils have positive effects on the atherosclerosis risk factors in rats fed a high-fat diet., Methods: Rats received experiment diets containing 20% fat and refined rapeseed oil or optimized rapeseed oils obtained with various processing technologies as lipid source. After 10 weeks of treatment, plasma was assayed for oxidative stress, lipid profiles and imflammation., Results: Micronutrients enhancement in optimized rapeseed oils significantly reduced plasma oxidative stress, as evaluated by the significant elevation in the activities of CAT and GPx as well as the level of GSH, and the significant decline in lipid peroxidation. Optimized rapeseed oil with the highest micronutrient contents obtained by microwave pretreatment-cold pressing reduced the levels of TG, TC and LDL-C as well as IL-6 and CRP in plasma., Conclusions: These results suggest that optimized rapeseed oils may contribute to prevent atherogenesis and make them very promising functional food in cardiovascular health promotion.
- Published
- 2014
- Full Text
- View/download PDF
42. A combination of flaxseed oil and astaxanthin alleviates atherosclerosis risk factors in high fat diet fed rats.
- Author
-
Xu J, Gao H, Zhang L, Chen C, Yang W, Deng Q, Huang Q, and Huang F
- Subjects
- Animals, Atherosclerosis etiology, Cholesterol blood, Cholesterol, LDL blood, Diet, High-Fat adverse effects, Lipid Peroxidation drug effects, Male, Oxidative Stress drug effects, Rats, Rats, Sprague-Dawley, Risk Factors, Triglycerides blood, Xanthophylls therapeutic use, Atherosclerosis drug therapy, Linseed Oil therapeutic use, Lipids blood
- Abstract
Background: Atherosclerosis is the most common pathologic process underlying cardiovascular disease. Both flaxseed oil (FO) and astaxanthin (ASX) are believed to benefit cardiovascular system. The combined effect of FO and ASX on the atherosclerosis risk factors in rats fed a high-fat diet was investigated., Methods: Astaxanthin was dissolved in flaxseed oil to a final concentration of 1g/kg (FO + ASX). Male Sprague-Dawley rats were fed a rodent diet contained 20% fat whose source was lard (HFD) or 75% lard and 25% FO + ASX (50 mg ASX/kg diet) or 50% lard and 50% FO + ASX (100 mg ASX/kg diet) or FO + ASX (200 mg ASX/kg diet) for 10 weeks., Results: The combination of FO and ASX significantly increased the antioxidant defense capacity and decreased lipid peroxidation in plasma. Evident decreases in the levels TG, TC and LDL-C contents, as well as IL-6 and CRP were also observed in plasma of FO and ASX fed rats., Conclusion: The combination of FO and ASX can improve oxidative stress, lipid abnormalities and inflammation, providing evidence that the combination of FO and ASX could be a promising functional food in cardiovascular health promotion.
- Published
- 2014
- Full Text
- View/download PDF
43. Flaxseed oil and alpha-lipoic acid combination ameliorates hepatic oxidative stress and lipid accumulation in comparison to lard.
- Author
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Xu J, Gao H, Song L, Yang W, Chen C, Deng Q, Huang Q, Yang J, and Huang F
- Subjects
- Animals, Diet, High-Fat, Dietary Fats administration & dosage, Fatty Liver pathology, Humans, Lipid Accumulation Product drug effects, Lipid Metabolism drug effects, Male, Non-alcoholic Fatty Liver Disease, Oxidative Stress drug effects, Rats, Fatty Liver drug therapy, Fatty Liver metabolism, Linseed Oil administration & dosage, Thioctic Acid administration & dosage
- Abstract
Background: Intake of high-fat diet is associated with increased non-alcoholic fatty liver disease (NAFLD). Hepatic lipid accumulation and oxidative stress are key pathophysiological mechanisms in NAFLD. Both flaxseed oil (FO) and α-lipoic acid (LA) exert potential benefit to NAFLD. The aim of this study was to determine the effect of the combination of FO and LA on hepatic lipid accumulation and oxidative stress in rats induced by high-fat diet., Methods: LA was dissolved in flaxseed oil to a final concentration of 8 g/kg (FO + LA). The rodent diet contained 20% fat. One-fifth of the fat was soybean oil and the others were lard (control group), or 75% lard and 25% FO + LA (L-FO + LA group), or 50% lard and 50% FO + LA (M-FO + LA group), or FO + LA (H-FO + LA group). Male Sprague-Dawley rats were fed for 10 weeks and then killed for liver collection., Results: Intake of high-fat lard caused a significant hepatic steatosis. Replacement with FO + LA was effective in reducing steatosis as well as total triglyceride and total cholesterol contents in liver. The combination of FO and LA also significantly elevated hepatic antioxidant defense capacities, as evaluated by the remarkable increase in the activities of SOD, CAT and GPx as well as the level of GSH, and the significant decline in lipid peroxidation., Conclusion: The combination of FO and LA may contribute to prevent fatty livers such as NAFLD by ameliorating hepatic lipid accumulation and oxidative stress.
- Published
- 2013
- Full Text
- View/download PDF
44. Micronutrients-fortified rapeseed oil improves hepatic lipid accumulation and oxidative stress in rats fed a high-fat diet.
- Author
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Xu J, Zhou X, Gao H, Chen C, Deng Q, Huang Q, Ma J, Wan Z, Yang J, and Huang F
- Subjects
- Animals, Antioxidants metabolism, Diet, High-Fat, Fatty Acids, Monounsaturated, Lipid Peroxidation drug effects, Liver metabolism, Liver pathology, Male, Oxidative Stress drug effects, Rapeseed Oil, Rats, Sprague-Dawley, Superoxide Dismutase metabolism, Tocopherols metabolism, Triglycerides metabolism, Food, Fortified, Lipid Metabolism drug effects, Liver drug effects, Micronutrients pharmacology, Plant Oils
- Abstract
Intake of high-fat diet is associated with increased fatty livers. Hepatic lipid accumulation and oxidative stress are key pathophysiological mechanisms in this disease. Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to hepatoprotection, but most of these micronutrients are removed by the traditional refining process. The purpose of the present study was to determine whether rapeseed oil fortified with these micronutrients can decrease hepatic lipid accumulation and oxidative stress induced by high-fat diet. Sprague-Dawley rats received rodent diet contained 20% fat whose source was refined rapeseed oil (RRO) or fortified RRO with low, middle and high quantities of these micronutrients for 10 weeks. Intake of RRO caused a remarkable hepatic steatosis. Micronutrients supplementation was effective in reducing steatosis as well as total triglyceride and total cholesterol contents in liver. These micronutrients also significantly increased hepatic antioxidant defense capacities, as evaluated by the significant elevation in the activities of SOD and GPx as well as the level of GSH, and the significant decline in lipid peroxidation. These findings suggest that rapeseed oil fortified with micronutrients polyphenols, tocopherols and phytosterols may contribute to prevent fatty livers such as nonalcoholic fatty liver disease by ameliorating hepatic lipid accumulation and oxidative stress.
- Published
- 2013
- Full Text
- View/download PDF
45. Rapeseed oil fortified with micronutrients reduces atherosclerosis risk factors in rats fed a high-fat diet.
- Author
-
Xu J, Zhou X, Deng Q, Huang Q, Yang J, and Huang F
- Subjects
- Animals, Antioxidants metabolism, Atherosclerosis blood, Atherosclerosis etiology, C-Reactive Protein metabolism, Catalase blood, Cholesterol, HDL blood, Cholesterol, LDL blood, Drug Evaluation, Preclinical, Enzyme Assays, Fatty Acids, Monounsaturated, Glutathione blood, Glutathione Peroxidase blood, Interleukin-6 blood, Lipid Peroxidation drug effects, Lipids blood, Male, Phytosterols pharmacology, Polyphenols pharmacology, Rapeseed Oil, Rats, Rats, Sprague-Dawley, Risk Factors, Superoxide Dismutase blood, Thiobarbituric Acid Reactive Substances metabolism, Tocopherols pharmacology, Atherosclerosis prevention & control, Dietary Fats adverse effects, Food, Fortified, Micronutrients pharmacology, Plant Oils pharmacology
- Abstract
Background: Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to cardiovascular system, but most of these micronutrients are removed by the refining process. The aim of this study was to determine the effect of rapeseed oil fortified with these micronutrients on the atherosclerosis risk factors in rats fed a high-fat diet., Methods: The rodent diet contained 20% fat whose source was refined rapeseed oil (RRO) or fortified refined rapeseed oil with low, middle and high quantities of these micronutrients (L-, M- and H-FRRO). Forty male SD rats were divided into four groups. One group received RRO diet and other groups received L-, M- and H-FRRO diet for 10 weeks., Results: Micronutrients supplementation significantly increased plasma antioxidant defense capacities, as evaluated by the significant elevation in the activities of GPx, CAT and SOD as well as the level of GSH, and the significant decline in lipid peroxidation. These micronutrients also reduced the plasma contents of TG, TC and LDL-C and increased the ratio of HDL-C/LDL-C. In addition, in parallel with the enhancement of these micronutrients, plasma levels of IL-6 and CRP declined remarkably., Conclusion: Rapeseed oil fortified with micronutrients polyphenols, tocopherols and phytosterols may contribute to prevent atherogenesis by ameliorating plasma oxidative stress, lipid profile and inflammation.
- Published
- 2011
- Full Text
- View/download PDF
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