Back to Search
Start Over
Purification and characterization of an antioxidant protein from Ginkgo biloba seeds
- Source :
-
Food Research International . Jan2010, Vol. 43 Issue 1, p86-94. 9p. - Publication Year :
- 2010
-
Abstract
- Abstract: A new antioxidation protein named G4b was purified from Ginkgo biloba seed albumin. The results of chemical colorimetry and chemiluminescence methods showed that the hemi-inhibitory concentration (IC50) of G4b on the hydroxyl radical were 78.1μg/mL and 115.1μg/mL, and the IC50 on DPPH was 100.7μg/mL, while the IC50 on the superoxide anion and DNA injury were 23μg/mL and 27μg/mL, respectively, and the abundance of sulfur amino acids and aromatic amino acids contained in G4b might contribute to its strong antioxidation properties. G4b is a kind of homogeneous and novel protein with a molecular mass of 29,247Da, consisting of two peptides with similar molecular weights linked by a disulfide bond. Result of β-elimination reaction showed that G4b is connected with a small quantity of polysaccharide by an oxygen-glucosidic bond. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09639969
- Volume :
- 43
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food Research International
- Publication Type :
- Academic Journal
- Accession number :
- 47149692
- Full Text :
- https://doi.org/10.1016/j.foodres.2009.08.015