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Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level

Authors :
Yang Wei
Fu Juan
Yu Miao
Huang Qingde
Wang Di
Xu Jiqu
Deng Qianchun
Yao Ping
Huang Fenghong
Liu Liegang
Source :
Lipids in Health and Disease, Vol 11, Iss 1, p 88 (2012)
Publication Year :
2012
Publisher :
BMC, 2012.

Abstract

Abstract Background The erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evidence regarding the protective effects of flaxseed oil, a natural product rich in n-3 fatty acid, on lipid peroxidation, antioxidative capacity and membrane deformation of erythrocytes exposed to high glucose is limited. Methods Human peripheral blood erythrocytes were isolated and treated with 50 mM glucose to mimic hyperglycemia in the absence or presence of three different doses of flaxseed oil (50, 100 or 200 μM) in the culture medium for 24 h. The malondialdehyde (MDA) and L-glutathione (GSH) were measured by HPLC and LC/MS respectively. The phospholipids symmetry and membrane fatty acid composition of human erythrocytes were detected by flow cytometry and gas chromatograph (GC). The morphology of human erythrocyte was illuminated by ultra scanning electron microscopy. Results Flaxseed oil attenuated hyperglycemia-induced increase of MDA and decrease of GSH in human erythrocytes. Human erythrocytes treated with flaxseed oil contained higher C22:5 and C22:6 than those in the 50 mM glucose control group, indicating that flaxseed oil could reduce lipid asymmetric distribution and membrane perturbation. The ultra scanning electron microscopy and flow cytometer have also indicated that flaxseed oil could protect the membrane of human erythrocytes from deformation at high glucose level. Conclusion The flaxseed oil supplementation may prevent lipid peroxidation and membrane dysfunction of human erythrocytes in hyperglycemia.

Details

Language :
English
ISSN :
1476511X
Volume :
11
Issue :
1
Database :
Directory of Open Access Journals
Journal :
Lipids in Health and Disease
Publication Type :
Academic Journal
Accession number :
edsdoj.818b546f84214693b774d75d65417e2f
Document Type :
article
Full Text :
https://doi.org/10.1186/1476-511X-11-88