25 results on '"Nakamura, Rika"'
Search Results
2. Elevated levels of autoantibodies against DNAJC2 in sera of patients with atherosclerotic diseases
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Yoshida, Yoichi, Zhang, Xiao-Meng, Wang, Hao, Machida, Toshio, Mine, Seiichiro, Kobayashi, Eiichi, Adachi, Akihiko, Matsutani, Tomoo, Kamitsukasa, Ikuo, Wada, Takeshi, Aotsuka, Akiyo, Iwase, Katsuro, Tomiyoshi, Go, Nakamura, Rika, Shinmen, Natsuko, Kuroda, Hideyuki, Takizawa, Hirotaka, Kashiwado, Koichi, Shin, Hideo, Akaogi, Yuichi, Shimada, Junichiro, Nishi, Eiichiro, Ohno, Mikiko, Takemoto, Minoru, Yokote, Koutaro, Kitamura, Kenichiro, Iwadate, Yasuo, and Hiwasa, Takaki
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- 2020
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3. Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke
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Hiwasa, Takaki, Wang, Hao, Goto, Ken-ichiro, Mine, Seiichiro, Machida, Toshio, Kobayashi, Eiichi, Yoshida, Yoichi, Adachi, Akihiko, Matsutani, Tomoo, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kunimatsu, Mitoshi, Kamitsukasa, Ikuo, Mori, Masahiro, Sugimoto, Kazuo, Uzawa, Akiyuki, Muto, Mayumi, Kuwabara, Satoshi, Kobayashi, Yoshio, Ohno, Mikiko, Nishi, Eiichiro, Hattori, Akiko, Yamamoto, Masashi, Maezawa, Yoshiro, Kobayashi, Kazuki, Ishibashi, Ryoichi, Takemoto, Minoru, Yokote, Koutaro, Takizawa, Hirotaka, Kishimoto, Takashi, Matsushita, Kazuyuki, Kobayashi, Sohei, Nomura, Fumio, Arasawa, Takahiro, Kagaya, Akiko, Maruyama, Tetsuro, Matsubara, Hisahiro, Tomiita, Minako, Hamanaka, Shinsaku, Imai, Yushi, Nakagawa, Tomoo, Kato, Naoya, Terada, Jiro, Matsumura, Takuma, Katsumata, Yusuke, Naito, Akira, Tanabe, Nobuhiro, Sakao, Seiichiro, Tatsumi, Koichiro, Ito, Masaaki, Shiratori, Fumiaki, Sumazaki, Makoto, Yajima, Satoshi, Shimada, Hideaki, Shirouzu, Mikako, Yokoyama, Shigeyuki, Kudo, Takashi, Doi, Hirofumi, Iwase, Katsuro, Ashino, Hiromi, Li, Shu-Yang, Kubota, Masaaki, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, and Iwadate, Yasuo
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- 2021
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4. Association of serum levels of antibodies against ALDOA and FH4 with transient ischemic attack and cerebral infarction
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Wang, Hao, Lu, Hao, Zhang, Xiao-Meng, Goto, Ken-ichiro, Kobayashi, Eiichi, Yoshida, Yoichi, Adachi, Akihiko, Matsutani, Tomoo, Iwadate, Yasuo, Mine, Seiichiro, Machida, Toshio, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kamitsukasa, Ikuo, Wada, Takeshi, Aotsuka, Akiyo, Sugimoto, Kazuo, Takizawa, Hirotaka, Kashiwado, Koichi, Shin, Hideo, Tomiyoshi, Go, Nakamura, Rika, Shinmen, Natsuko, Kuroda, Hideyuki, Xu, Anding, and Hiwasa, Takaki
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- 2021
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5. Serum anti-AP3D1 antibodies are risk factors for acute ischemic stroke related with atherosclerosis
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Li, Shu-Yang, Yoshida, Yoichi, Kobayashi, Eiichi, Kubota, Masaaki, Matsutani, Tomoo, Mine, Seiichiro, Machida, Toshio, Maezawa, Yoshiro, Takemoto, Minoru, Yokote, Koutaro, Kobayashi, Yoshio, Takizawa, Hirotaka, Sata, Mizuki, Yamagishi, Kazumasa, Iso, Hiroyasu, Sawada, Norie, Tsugane, Shoichiro, Kobayashi, Sohei, Matsushita, Kazuyuki, Nomura, Fumio, Matsubara, Hisahiro, Sumazaki, Makoto, Ito, Masaaki, Yajima, Satoshi, Shimada, Hideaki, Iwase, Katsuro, Ashino, Hiromi, Wang, Hao, Goto, Kenichiro, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, Iwadate, Yasuo, and Hiwasa, Takaki
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- 2021
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6. Evaluation of the Luciferase Assay-Based In Vitro Elicitation Test for Serum IgE
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Nakamura, Ryosuke, Ishiwatari, Ayano, Higuchi, Masakazu, Uchida, Yoshimi, Nakamura, Rika, Kawakami, Hiroshi, Urisu, Atsuo, and Teshima, Reiko
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- 2012
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7. Emergence of the super antenna effect in mixed crystals of ytterbium and lutetium complexes showing near-infrared luminescence.
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Masuya-Suzuki, Atsuko, Goto, Satoshi, Nakamura, Rika, Karashimada, Ryunosuke, Kubota, Yasuhiro, Tsunashima, Ryo, and Iki, Nobuhiko
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- 2022
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8. An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay
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Watanabe, Seiji, Tanaka, Atsushi, Fujii, Shunsaku, Mizunuma, Hideki, Fukui, Atsushi, Fukuhara, Rie, Nakamura, Rika, Yamada, Kenichi, Tanaka, Izumi, Awata, Shoichiro, and Nagayoshi, Motoi
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- 2011
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9. Erythrocytes counteract the negative effects of female ageing on mouse preimplantation embryo development and blastocyst formation
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Fukuhara, Rie, Fujii, Shunsaku, Nakamura, Rika, Yuzawa, Ei, Kimura, Hidetaka, Fukui, Atsushi, and Mizunuma, Hideki
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- 2008
10. Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis
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Nakamura, Rika, Nakamura, Ryosuke, Nakano, Mikiharu, Arisawa, Kenjiro, Ezaki, Ryo, Horiuchi, Hiroyuki, and Teshima, Reiko
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FOOD allergy , *GENETICALLY modified foods , *CHICKEN as food , *MEAT , *SEROLOGY , *GEL electrophoresis , *IMMUNOGLOBULIN E , *GREEN fluorescent protein ,ANIMAL product analysis - Abstract
Abstract: Genetically modified (GM) foods must be tested for safety, including by allergenicity tests to ensure that they do not contain new allergens or higher concentrations of known allergens than the same non-GM foods. In this study experimentally developed EGFP-transgenic chickens were used and evaluated the allergenicity of meat from the chicken based on a serological and two-dimensional difference gel electrophoresis (2D-DIGE) analysis. For the serological analysis, a Western blotting with allergen-specific antibodies and a proteomic analysis of chicken meat allergens with patients’ sera, a so-called allergenome analysis, were used. The allergenome analysis allowed us to identify five IgE-binding proteins in chicken meat, including a known allergen, chicken serum albumin, and no qualitative difference in their expressions between the GM and non-GM chicken meat was found. Results of the 2D-DIGE analysis showed that none of the IgE-binding proteins in chicken meat were significantly changed in expression levels between non-GM and GM chicken, and only 3 of the 1500 soluble protein spots including green fluorescence protein were markedly different as a result of gene transfer. These above results showed that the combination of serological and 2D-DIGE analysis is a valid method of evaluating quality and quantity of allergens in GM foods. [Copyright &y& Elsevier]
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- 2010
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11. The PC motif : a novel and evolutionarily conserved sequence involved in interaction between p40 phox and p67phox, SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase.
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Nakamura, Rika, Sumimoto, Hideki, Mizuki, Kazuhito, Hata, Kenichiro, Ago, Tetsuro, Kitajima, Shigetaka, Takeshige, Koichiro, Sakaki, Yoshiyuki, and Ito, Takashi
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OXIDASES , *YEAST , *PROTEINS - Abstract
The superoxide-generating NADPH oxidase, dormant in resting phagocytes, is activated during phagocytosis following assembly of the membrane-integrated protein cytochrome b558 and cytosolic factors. Among the latter are the three proteins containing Src homology 3 (SH3) domains, p67phox, p47phox and p40 phox. While the first two factors are indispensable for the activity, p40 phox is tightly associated with p67phox in resting cells and is suggested to have some modulatory role. Here we describe a systematic analysis of the interaction between p40 phox and p67phox using the yeast two-hybrid system and in vitro binding assays with recombinant proteins. Both methods unequivocally showed that the minimum requirements for stable interaction are the C-terminal region of p40 phox and the region between the two SH3 domains of p67phox. This interaction is maintained even in the presence of anionic amphiphiles used for the activation of the NADPH oxidase, raising a possibility that it mediates constitutive association of the two factors in both resting and activated cells. The C-terminal region of p40 phox responsible for the interaction contains a characteristic stretch of amino acids designated as the PC motif, that also exists in other signal-transducing proteins from yeast to human. Intensive site-directed mutagenesis to the motif in p40 phox revealed that it plays a critical role in the binding to p67 phox. Thus the PC motif appears to represent a novel module for protein-protein interaction used in a variety of signaling pathways. [ABSTRACT FROM AUTHOR]
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- 1998
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12. Circulating Anti-Sorting Nexins 16 Antibodies as an Emerging Biomarker of Coronary Artery Disease in Patients with Obstructive Sleep Apnea.
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Katsumata, Yusuke, Terada, Jiro, Matsumura, Takuma, Koshikawa, Ken, Sakao, Seiichiro, Tomiyoshi, Go, Shinmen, Natsuko, Nakamura, Rika, Kuroda, Hideyuki, Nagashima, Kengo, Kobayashi, Yoshio, Kobayashi, Eiichi, Iwadate, Yasuo, Zhang, Xiao-Meng, Hiwasa, Takaki, and Tatsumi, Koichiro
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SLEEP apnea syndromes ,CORONARY disease ,ACUTE coronary syndrome ,CARDIOVASCULAR diseases risk factors ,LOGISTIC regression analysis ,DYSLIPIDEMIA - Abstract
Biomarkers are not available for monitoring the onset and progression of coronary artery disease (CAD) in patients with obstructive sleep apnea (OSA), a major risk factor for arteriosclerotic cardiovascular diseases. This study aimed to test for correlation between circulating anti-Sorting Nexins 16 antibody (SNX16-Ab) levels, CAD history and clinical parameters of patients with OSA. Sixty-four healthy donors, 82 adults with OSA, and 96 with acute coronary syndrome (ACS) were studied. Serum samples were collected at diagnostic polysomnography in the OSA group or at the disease onset in the ACS group. Serum SNX16-Ab levels were measured by amplified luminescence proximity homogeneous assay (AlphaLISA), and correlation between SNX16-Ab levels and clinical parameters was analyzed. SNX16-Ab levels and apnea-hypopnea index (AHI) were weakly correlated. Additionally, logistic regression analyses of OSA group identified that elevated SNX16-Ab level associated with the history of CAD. Circulating SNX16-Ab could increase during CAD pathogenesis in patients with OSA. Further prospective studies are required to prove the predictive potential of SNX16-Ab level in CAD onset of patients with OSA. [ABSTRACT FROM AUTHOR]
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- 2020
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13. Small Thoracic Disk Herniation without Spinal Stenosis Presenting with Acute Myelopathy: Three Case Reports.
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Nakamura R and Takai K
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We herein describe three patients with thoracic disk herniation (TDH) that presented with acute myelopathy at the Tokyo Metropolitan Neurological Hospital between 2014 and 2021 (age range, 45-76 years; male/female ratio = 1:2), with a focus on the mechanisms underlying their development. All patients had sudden-onset gait disturbance due to acute nontraumatic paraparesis. The specialties of the doctors at the first hospital were neurology and orthopedic surgery. TDH was overlooked at the first hospital, and the patients were referred to our hospital. The TDH in all cases was of the central type; however, since they were small, no spinal stenosis was observed. The key feature of all three cases is the small anterior deformation of the spinal cord, making a vascular etiology for the symptoms more plausible than a compressive etiology. After a follow-up of several months or years, two out of three patients underwent surgery with the use of the transfacet pedicle-sparing approach due to residual symptoms. Intraoperative ultrasonography showed that the spinal cord was anchored to TDH by the dural attachment of dentate ligaments. The physical relationship between the dentate ligaments and TDH may be associated with the vascular cause of the symptoms of small TDH., Competing Interests: The authors declare that they have no conflict of interest., (© 2023 The Japan Neurosurgical Society.)
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- 2023
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14. Successful Mechanical Thrombectomy for Isolated Internal Carotid Artery Occlusion in a Patient with Monocular Blindness: A Case Report.
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Dofuku S, Sato M, Aoka T, Nakamura R, Ohara K, and Ota T
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We report a rare case of isolated internal carotid artery occlusion complicated by central retinal artery occlusion that was successfully treated with mechanical thrombectomy for internal carotid artery occlusion. A 59-year-old man visited the emergency room because of right monocular blindness. Magnetic resonance imaging showed multiple acute small embolic infarctions in the right frontal lobe, and magnetic resonance angiography revealed right internal carotid artery occlusion without the associated occlusion of the circle of Willis, which indicates the patency of the anterior and middle cerebral arteries. An electrocardiogram showed atrial fibrillation. Therefore, we performed mechanical thrombectomy with a stent retriever under continuous manual aspiration with a balloon-guiding catheter and confirmed complete recanalization, anterograde flow in the right ophthalmic artery, and retinal brush. The procedure was completed without complications, and the patient noticed an improvement in visual acuity immediately after the procedure. When a patient with atrial fibrillation complains of monocular blindness, it is important to consider internal carotid artery occlusion due to cardioembolism, to perform an examination promptly, and to consider early treatment, including mechanical thrombectomy., Competing Interests: There are no competing interests to declare., (© 2023 The Japan Neurosurgical Society.)
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- 2023
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15. GADD34 activates p53 and may have utility as a marker of atherosclerosis.
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Tomiyoshi G, Nakamura R, Shinmen N, Yoshida Y, Mine S, Machida T, Iwase K, Iwadate Y, Hiwasa T, and Kuroda H
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We previously identified growth arrest and DNA-damage-inducible gene 34 (GADD34) as a marker of ischemic stroke. In the present study, serum levels of anti-GADD34 antibodies were found to be significantly higher in patients with acute ischemic stroke or chronic kidney disease compared to healthy donors. We then examined the biological function of GADD34 by transfection into U2OS human osteosarcoma and U87 human glioblastoma cells. Knockdown of GADD34 by siRNA resulted in enhanced cell proliferation, which was reversed by co-knockdown of MDM2. Luciferase reporter assays revealed that the transactivation ability of p53 enhanced by genotoxic anticancer drugs such as camptothecin and etoposide was further potentiated by enforced expression of GADD34 but attenuated by co-transfection with p53 shRNA expression plasmids. Western blotting demonstrated increased p53 protein levels after treatment with camptothecin, which was also potentiated by GADD34 but suppressed by GADD34 siRNA, ATM siRNA, and ATM inhibitor wortmannin. GADD34 levels also increased in response to treatment with camptothecin or adriamycin, and this increase was attenuated by MDM2 siRNA. Immunoprecipitation with anti-GADD34 antibody followed by Western blotting with anti-MDM2 antibodies indicated ubiquitination of GADD34 is mediated by MDM2. Accordingly, GADD34 may function as a ubiquitination decoy to reduce p53 ubiquitination and increase p53 protein levels. Increased neuronal cell death due to activation of p53 by GADD34 may account for the elevated serum levels of anti-GADD34 antibodies observed in patients with acute ischemic stroke., Competing Interests: This work was performed in collaboration with Fujikura Kasei Co., Ltd. GT, RN, NS, and HK are employee of Fujikura Kasei Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Tomiyoshi, Nakamura, Shinmen, Yoshida, Mine, Machida, Iwase, Iwadate, Hiwasa and Kuroda.)
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- 2023
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16. Sequential Middle Meningeal Artery Embolization after Burr Hole Surgery for Recurrent Chronic Subdural Hematoma.
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Dofuku S, Sato D, Nakamura R, Ogawa S, Torazawa S, Sato M, and Ota T
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- Humans, Male, Aged, 80 and over, Female, Retrospective Studies, Meningeal Arteries diagnostic imaging, Meningeal Arteries surgery, Trephining, Craniotomy methods, Hematoma, Subdural, Chronic diagnostic imaging, Hematoma, Subdural, Chronic surgery, Embolization, Therapeutic methods, Enbucrilate
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Middle meningeal artery embolization (MMAE) for chronic subdural hematoma (CSDH) is a novel, minimally invasive treatment. The indications and treatment practices for MMAE are variable and remain controversial. This study aimed to evaluate a strategy involving sequential MMAE after burr hole surgery for treating recurrent CSDH. We performed a retrospective analysis of data from consecutive patients who had undergone MMAE using liquid embolic agents within approximately 2 weeks after burr hole surgery for recurrent CSDH from September 2020 to March 2022. We analyzed patient characteristics, procedural details, CSDH recurrence after MMAE, surgical rescue, and complications. Six of the nine patients who underwent MMAE for CSDH recurrence were male, and the median age was 85 (range, 70-94) years. Five of the nine patients were being administered antithrombotic agents. The median duration between the burr hole surgery and MMAE procedure was 10 (range, 3-25) days. Anterior and posterior convexity branches were targeted for embolization using low-concentration N-butyl cyanoacrylate (NBCA), and the abnormal vascular networks with a cotton wool appearance disappeared after embolization in all cases. The NBCA distribution was observed by high-resolution computed tomography during the procedure; in three of nine cases, the NBCA penetrated not only the MMA but also the inner membrane. No recurrence, surgical rescue, or complications were observed in any patient during the median follow-up period of 3 months. As a minimally invasive treatment for recurrent CSDH, sequential MMAE after burr hole surgery may be a safe and effective option for preventing recurrence.
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- 2023
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17. Serum anti-LRPAP1 is a common biomarker for digestive organ cancers and atherosclerotic diseases.
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Sumazaki M, Shimada H, Ito M, Shiratori F, Kobayashi E, Yoshida Y, Adachi A, Matsutani T, Iwadate Y, Mine S, Machida T, Kamitsukasa I, Mori M, Sugimoto K, Uzawa A, Kuwabara S, Kobayashi Y, Ohno M, Nishi E, Maezawa Y, Takemoto M, Yokote K, Takizawa H, Kashiwado K, Shin H, Kishimoto T, Matsushita K, Kobayashi S, Nakamura R, Shinmen N, Kuroda H, Zhang XM, Wang H, Goto KI, and Hiwasa T
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- Acute Disease, Biomarkers blood, Colorectal Neoplasms blood, Colorectal Neoplasms immunology, DNA, Complementary, Esophageal Neoplasms immunology, Esophageal Squamous Cell Carcinoma immunology, Humans, Immunoenzyme Techniques, Ischemic Stroke immunology, Neoplasm Proteins immunology, Esophageal Neoplasms blood, Esophageal Squamous Cell Carcinoma blood, Immunoglobulin G blood, Ischemic Stroke blood, LDL-Receptor Related Protein-Associated Protein immunology
- Abstract
Some cancers are related to atherosclerotic diseases; therefore, these two types of disease may share some antibody biomarkers in common. To investigate this, a first screening of sera was performed from patients with esophageal squamous cell carcinoma (ESCC) or acute ischemic stroke (AIS) for serological identification of antigens using recombinant cDNA expression cloning (SEREX). The amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method, which incorporates glutathione donor beads and anti-human IgG acceptor beads, was used to evaluate serum antibody levels. SEREX screening identified low-density lipoprotein receptor-related protein-associated protein 1 (LRPAP1) as a target antigen of serum IgG antibodies in the sera of patients with ESCC or AIS. Antigens, including recombinant glutathione S-transferase-fused LRPAP1 protein, were prepared to examine serum antibody levels. AlphaLISA revealed significantly higher antibody levels against the LRPAP1 protein in patients with solid cancers such as ESCC and colorectal carcinoma and some atherosclerosis-related diseases such as AIS and diabetes mellitus compared with healthy donors. Correlation analysis revealed that the elevated serum antibody levels against LRPAP1 were associated with smoking, a well-known risk factor for both cancer and atherosclerosis. Serum LRPAP1 antibody is therefore a common marker for the early diagnosis of some cancers and atherosclerotic diseases and may reflect diseases caused by habitual smoking., (© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.)
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- 2020
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18. Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension.
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Li SY, Yoshida Y, Kobayashi E, Adachi A, Hirono S, Matsutani T, Mine S, Machida T, Ohno M, Nishi E, Maezawa Y, Takemoto M, Yokote K, Kitamura K, Sumazaki M, Ito M, Shimada H, Takizawa H, Kashiwado K, Tomiyoshi G, Shinmen N, Nakamura R, Kuroda H, Zhang XM, Wang H, Goto K, Iwadate Y, and Hiwasa T
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- Aged, Biomarkers blood, Brain Ischemia blood, Brain Ischemia etiology, Brain Ischemia metabolism, Cardiovascular Diseases etiology, Cardiovascular Diseases metabolism, Diabetes Mellitus etiology, Diabetes Mellitus metabolism, Digestive System Neoplasms etiology, Digestive System Neoplasms metabolism, Female, Humans, Ischemic Stroke etiology, Ischemic Stroke metabolism, Male, Middle Aged, Renal Insufficiency, Chronic etiology, Renal Insufficiency, Chronic metabolism, Antibodies blood, Cardiovascular Diseases blood, Diabetes Mellitus blood, Digestive System Neoplasms blood, Ischemic Stroke blood, Renal Insufficiency, Chronic blood, Repressor Proteins metabolism
- Abstract
The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs‑like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S‑transferase‑fused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homogeneous assay‑linked immunosorbent assay, which incorporates glutathione‑donor beads and anti‑human‑IgG‑acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension‑induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers.
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- 2020
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19. Onco-testicular sperm extraction (Onco-TESE) from a single testis with metachronous bilateral testicular cancer: a case report.
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Hamano I, Hatakeyama S, Nakamura R, Fukuhara R, Noro D, Seino H, Yoneyama T, Hashimoto Y, Koie T, Yokoyama Y, and Ohyama C
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Background: Although oncologic testicular sperm extraction (onco-TESE) has been increasingly practiced, the evidence of onco-TESE performed in patients with testicular cancer is insufficient. Furthermore, in bilateral testicular cancer, accounting for 0.5%-1% of testicular cancers, onco-TESE is more challenging and has been insufficiently reported., Case Presentation: Here we report the case of a 25-year-old man who underwent onco-TESE from his residual single testis with a nonseminomatous germ cell tumor that occurred 5 years after orchiectomy of the contralateral testis. A second orchiectomy and simultaneous TESE from the noncancerous testicular tissue were performed. The pathological diagnosis was germ cell tumors, tumors of more than one histological type (embryonal carcinoma, immature teratoma, yolk sac tumor, seminoma, and choriocarcinoma; pT1N0M0). The patient subsequently married and hoped for fatherhood 3 years later. Whereas histological diagnosis of the normal testicular tissue was Johnsen score 6 (maturation arrest), morphologically normal and motile sperms were successfully retrieved from thawed TESE samples and used for multiple cycles of intracytoplasmic sperm injection. Although the conception has not been succeeded to date, ICSI attempts have been continuing., Conclusion: This case demonstrates the effectiveness of onco-TESE for challenging cases such as bilateral and nonseminmatous testicular cancer., Competing Interests: Not applicableWritten informed consent for publication of clinical details and clinical images of this case report was obtained from the patient.The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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- 2018
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20. Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction.
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Wang H, Zhang XM, Tomiyoshi G, Nakamura R, Shinmen N, Kuroda H, Kimura R, Mine S, Kamitsukasa I, Wada T, Aotsuka A, Yoshida Y, Kobayashi E, Matsutani T, Iwadate Y, Sugimoto K, Mori M, Uzawa A, Muto M, Kuwabara S, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Yokote K, Ohno M, Chen PM, Nishi E, Ono K, Kimura T, Machida T, Takizawa H, Kashiwado K, Shimada H, Ito M, Goto KI, Iwase K, Ashino H, Taira A, Arita E, Takiguchi M, and Hiwasa T
- Abstract
Transient ischemic attack (TIA) is a predictor for cerebral infarction (CI), and early diagnosis of TIA is extremely important for the prevention of CI. We set out to identify novel antibody biomarkers for TIA and CI, and detected matrix metalloproteinase 1 (MMP1), chromobox homolog 1 (CBX1), and chromobox homolog 5 (CBX5) as candidate antigens using serological identification of antigens by recombinant cDNA expression cloning (SEREX) and Western blotting to confirm the presence of serum antibodies against the antigens. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) revealed that serum antibody levels were significantly higher in patients with TIA or acute-phase CI (aCI) compared with healthy donors ( P < 0.01). Spearman's correlation analysis and multivariate logistic regression analysis demonstrated that levels of anti-MMP1, anti-CBX1, and anti-CBX5 antibodies were associated with age, cigarette-smoking habits, and blood pressure. Thus, serum levels of antibodies against MMP1, CBX1, and CBX5 could potentially serve as useful tools for diagnosing TIA and predicting the onset of aCI., Competing Interests: CONFLICTS OF INTEREST This work was performed in collaboration with Fujikura Kasei Co., Ltd. Go Tomiyoshi, Rika Nakamura, Natsuko Shinmen, and Hideyuki Kuroda are employees of Fujikura Kasei Co., Ltd.
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- 2017
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21. Elevation of autoantibody level against PDCD11 in patients with transient ischemic attack.
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Yoshida Y, Wang H, Hiwasa T, Machida T, Kobayashi E, Mine S, Tomiyoshi G, Nakamura R, Shinmen N, Kuroda H, Takizawa H, Kashiwado K, Kamitsukasa I, Shin H, Wada T, Aotsuka A, Nishi E, Ohno M, Takemoto M, Yokote K, Takahashi S, Matsushima J, Zhang XM, Takiguchi M, and Iwadate Y
- Abstract
Background: Disease specific autoantibodies have been detected in the sera of patients with atherosclerosis-related diseases, such as cerebral infarction, cardiovascular disease. In the present study, we aimed to identify novel autoantibodies responsible for transient ischemic attack (TIA), a prodromal condition for cerebral infarction., Methods: To identify candidate antigens, we screened a human aortic endothelial cell cDNA library using sera from 20 patients with TIA. Serum antibody levels were measured using amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in 2 independent patient/healthy donor (HD) cohorts ( n = 192 and n = 906 in the second screening and validation cohort, respectively)., Results: First screening identified 3 candidate antigens. Of these, programmed cell death 11 (PDCD11) was determined to be associated with stroke ( p < 0.0001), as evidenced from the second screening using AlphaLISA. The validation cohort revealed significantly higher antibody levels against PDCD11 (PDCD11-Ab levels) in patients with TIA than in HDs. Multivariate logistic regression analysis indicated that the predictive value of PDCD11-Ab levels for TIA [Odds ratio (OR): 2.44, 95% confidence interval (CI): 1.33-4.57, p = 0.0039] was not inferior to other known risk factors for ischemic stroke, including age (OR: 4.97, 95% CI: 2.67-9.48, p < 0.0001); hypertension (OR: 3.21, 95% CI: 1.76-5.86, p = 0.0001); and diabetes (OR: 4.31, 95% CI: 1.74-11.2, p = 0.0015)., Conclusion: Serum PDCD11-Ab level may serve as a potential biomarker for TIA., Competing Interests: CONFLICTS OF INTEREST This work was performed in collaboration with Fujikura Kasei Co., Ltd. GT, RN, NS and HK are employees of Fujikura Kasei Co., Ltd.
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- 2017
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22. Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus.
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Hiwasa T, Zhang XM, Kimura R, Ohno M, Chen PM, Nishi E, Ono K, Kimura T, Kamitsukasa I, Wada T, Aotsuka A, Mine S, Takizawa H, Kashiwado K, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Yokote K, Nakamura R, Tomiyoshi G, Shinmen N, and Kuroda H
- Abstract
Adiponectin secreted from the adipocytes plays pleiotropic, anti-atherosclerotic roles, such as enhancement of insulin secretion and an increase in energy expenditure. The measurement of levels of circulating adiponectin is useful to evaluate the progression of atherosclerosis-related diseases, such as coronary artery disease (CAD), cerebral infarction (CI) and diabetes mellitus (DM). We examined the serum antibody levels against recombinant adiponectin protein via the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method. The results revealed that the antibody levels were significantly higher in patients with CAD, CI and type 2 DM, than in healthy donors. Receiver operating curve analysis showed that the sensitivity was in a range of 41-48% for CAD, CI and DM. Thus, the serum anti-adiponectin antibody levels could be a common marker for atherosclerosis-related diseases., Competing Interests: This work was performed in collaboration with Fujikura Kasei Co., Ltd. and Celish Fd Inc. RN, GT, NS and HK are employees of Fujikura Kasei Co., Ltd.
- Published
- 2016
- Full Text
- View/download PDF
23. Hemophagocytic lymphohistiocytosis in a rheumatoid arthritis patient treated with infliximab.
- Author
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Oda Y, Urushidani Y, Ooi S, Endoh A, Nakamura R, Adachi K, and Fukushima H
- Subjects
- Aged, Anti-Bacterial Agents therapeutic use, Antibodies, Monoclonal therapeutic use, Antirheumatic Agents therapeutic use, Arthritis, Rheumatoid drug therapy, Arthritis, Rheumatoid immunology, Bone Marrow pathology, Escherichia coli Infections drug therapy, Fatal Outcome, Female, Histiocytes pathology, Humans, Immunocompromised Host, Immunoglobulins, Intravenous therapeutic use, Immunosuppressive Agents therapeutic use, Infliximab, Meropenem, Methylprednisolone therapeutic use, Pyelonephritis drug therapy, Thienamycins therapeutic use, Antibodies, Monoclonal adverse effects, Antirheumatic Agents adverse effects, Arthritis, Rheumatoid complications, Escherichia coli Infections complications, Immunosuppressive Agents adverse effects, Lymphohistiocytosis, Hemophagocytic etiology, Pyelonephritis complications
- Abstract
Hemophagocytic lymphohistiocytosis (HLH) is a rare condition with high mortality. We report a case of a 74-year-old woman with rheumatoid arthritis who developed HLH secondary to pyelonephritis due to Escherichia coli infection following infliximab treatment. Bone marrow aspiration showed proliferation of histiocytes with hemophagocytosis. The patient died despite treatment with intravenous antibiotics intravenous methylprednisolone and intravenous immunoglobulin. Cytokine levels were measured and are discussed.
- Published
- 2012
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- View/download PDF
24. Immunoproteomic and two-dimensional difference gel electrophoresis analysis of Arabidopsis dehydration response element-binding protein 1A (DREB1A)-transgenic potato.
- Author
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Nakamura R, Satoh R, Nakamura R, Shimazaki T, Kasuga M, Yamaguchi-Shinozaki K, Kikuchi A, Watanabe KN, and Teshima R
- Subjects
- Allergens genetics, Allergens immunology, Carboxylic Ester Hydrolases genetics, Carboxylic Ester Hydrolases immunology, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Food Hypersensitivity blood, Humans, Immunoblotting, Plant Proteins genetics, Plant Proteins immunology, Protein Binding, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Transfection, Transgenes, Arabidopsis Proteins genetics, Food Hypersensitivity immunology, Immunoglobulin E blood, Plants, Genetically Modified adverse effects, Plants, Genetically Modified genetics, Plants, Genetically Modified immunology, Solanum tuberosum adverse effects, Solanum tuberosum genetics, Solanum tuberosum immunology, Transcription Factors genetics
- Abstract
To produce crops that are more tolerant to stresses such as heat, cold, and salt, transgenic plants have been produced those express stress-associated proteins. In this study, we used immunoproteomic and two-dimensional difference gel electrophoresis (2D-DIGE) methods to investigate the allergenicity of transgenic potatoes expressing Arabidopsis DREB1A (dehydration responsive element-binding protein 1A), driven by the rd29A promoter or the 35S promoter. Immunoproteomic analysis using sera from potato-allergic patients revealed several immunoglobulin E (IgE)-binding protein spots. The patterns of protein binding were almost the same between transgenic and non-transgenic potatoes. The IgE-binding proteins in potato were identified as patatin precursors, a segment of serine protease inhibitor 2, and proteinase inhibitor II by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) MS/MS. 2D-DIGE analysis revealed several differences in protein expression between non-transgenic potato and transgenic potato; those showing increased expression in transgenic potatoes were identified as precursors of patatin, a major potato allergen, and those showing decreased expression in transgenic potatoes were identified as lipoxygenase and glycogen (starch) synthase. These results suggested that transgenic potatoes may express slightly higher levels of allergens, but their IgE-binding patterns were almost the same as those of control potatoes. Further research on changes in protein expressions in response to environmental factors is required to confirm whether the differences observed in this study are due to gene transfection, rather than environmental factors.
- Published
- 2010
- Full Text
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25. Immunological characterization and mutational analysis of the recombinant protein BWp16, a major allergen in buckwheat.
- Author
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Satoh R, Koyano S, Takagi K, Nakamura R, Teshima R, and Sawada J
- Subjects
- Adolescent, Adult, Amino Acid Sequence, Blotting, Western, Child, Child, Preschool, Cysteine genetics, DNA Mutational Analysis, DNA, Complementary biosynthesis, DNA, Complementary genetics, Enzyme-Linked Immunosorbent Assay, Escherichia coli genetics, Female, Humans, Hydrolysis, Immunoglobulin E metabolism, Infant, Male, Molecular Sequence Data, Mutagenesis, Site-Directed, Pepsin A chemistry, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins immunology, Antigens, Plant genetics, Antigens, Plant immunology, Fagopyrum genetics, Fagopyrum immunology
- Abstract
Buckwheat allergy is one of the most critical diseases manifested by severe and dangerous symptoms in Japan and other countries. We previously isolated the cDNA encoding protein BWp16, a member of the 2S albumin family with a conserved motif of 8 cysteine (Cys) residues. Comparison of the deduced amino acid sequences of BWp16 and related proteins in the 2S albumin family showed similarities between BWp16 and BW 8-kDa from buckwheat, Ara h 6 from peanuts and Ric c 1 from castor bean. Purified recombinant BWp16 (rBWp16) expressed in Escherichia coli was recognized by >80% of sera from patients with positive for IgE binding to buckwheat. Mutational analysis of rBWp16 revealed that 7 out of 10 mutants in the Cys residues showed weaker IgE binding to patient's serum than wild-type rBWp16 (rBWp16 WT). Mutations of Cys65 and Cys66 in rBWp16 decreased the pepsin digestibility of the protein, and an ELISA inhibition assay revealed a weaker inhibitory effect of rBWp16 C65S than that of rBWp16 WT. These results suggest that the Cys residues, especially Cys65, are involved in the allergenicity of rBWp16. Our findings provide new evidence for the role of Cys residues in 2S albumin family proteins and open the door to the production of hypoallergens and application to safe diagnostic methods and allergen-specific immunotherapy of buckwheat allergy.
- Published
- 2008
- Full Text
- View/download PDF
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