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Association between serum anti‑ASXL2 antibody levels and acute ischemic stroke, acute myocardial infarction, diabetes mellitus, chronic kidney disease and digestive organ cancer, and their possible association with atherosclerosis and hypertension.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2020 Oct; Vol. 46 (4), pp. 1274-1288. Date of Electronic Publication: 2020 Jul 29. - Publication Year :
- 2020
-
Abstract
- The aim of the present study was to identify novel antibody markers for the early diagnosis of atherosclerosis in order to improve the prognosis of patients at risk for acute ischemic stroke (AIS) and acute myocardial infarction (AMI). A first screening involved the serological identification of antigens by recombinant cDNA expression cloning and identified additional sex combs‑like 2 (ASXL2) as a target antigen recognized by serum IgG antibodies in the sera of patients with atherosclerosis. Antigens, including the recombinant glutathione S‑transferase‑fused ASXL2 protein and its synthetic peptide were then prepared to examine serum antibody levels. Amplified luminescence proximity homogeneous assay‑linked immunosorbent assay, which incorporates glutathione‑donor beads and anti‑human‑IgG‑acceptor beads, revealed significantly higher serum antibody levels against the ASXL2 protein and its peptide in the patients with AIS, diabetes mellitus, AMI, chronic kidney disease, esophageal squamous cell carcinoma, or colorectal carcinoma compared with those in healthy donors. The ASXL2 antibody levels were well associated with hypertension complication, but not with sex, body mass index, habitual smoking, or alcohol intake. These results suggest that the serum ASXL2 antibody marker can discriminate between hypertension‑induced atherosclerotic AIS and AMI, as well as a number of digestive organ cancers.
- Subjects :
- Aged
Biomarkers blood
Brain Ischemia blood
Brain Ischemia etiology
Brain Ischemia metabolism
Cardiovascular Diseases etiology
Cardiovascular Diseases metabolism
Diabetes Mellitus etiology
Diabetes Mellitus metabolism
Digestive System Neoplasms etiology
Digestive System Neoplasms metabolism
Female
Humans
Ischemic Stroke etiology
Ischemic Stroke metabolism
Male
Middle Aged
Renal Insufficiency, Chronic etiology
Renal Insufficiency, Chronic metabolism
Antibodies blood
Cardiovascular Diseases blood
Diabetes Mellitus blood
Digestive System Neoplasms blood
Ischemic Stroke blood
Renal Insufficiency, Chronic blood
Repressor Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 46
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 32945427
- Full Text :
- https://doi.org/10.3892/ijmm.2020.4690