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Serum anti-LRPAP1 is a common biomarker for digestive organ cancers and atherosclerotic diseases.
- Source :
-
Cancer science [Cancer Sci] 2020 Dec; Vol. 111 (12), pp. 4453-4464. Date of Electronic Publication: 2020 Oct 04. - Publication Year :
- 2020
-
Abstract
- Some cancers are related to atherosclerotic diseases; therefore, these two types of disease may share some antibody biomarkers in common. To investigate this, a first screening of sera was performed from patients with esophageal squamous cell carcinoma (ESCC) or acute ischemic stroke (AIS) for serological identification of antigens using recombinant cDNA expression cloning (SEREX). The amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method, which incorporates glutathione donor beads and anti-human IgG acceptor beads, was used to evaluate serum antibody levels. SEREX screening identified low-density lipoprotein receptor-related protein-associated protein 1 (LRPAP1) as a target antigen of serum IgG antibodies in the sera of patients with ESCC or AIS. Antigens, including recombinant glutathione S-transferase-fused LRPAP1 protein, were prepared to examine serum antibody levels. AlphaLISA revealed significantly higher antibody levels against the LRPAP1 protein in patients with solid cancers such as ESCC and colorectal carcinoma and some atherosclerosis-related diseases such as AIS and diabetes mellitus compared with healthy donors. Correlation analysis revealed that the elevated serum antibody levels against LRPAP1 were associated with smoking, a well-known risk factor for both cancer and atherosclerosis. Serum LRPAP1 antibody is therefore a common marker for the early diagnosis of some cancers and atherosclerotic diseases and may reflect diseases caused by habitual smoking.<br /> (© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.)
- Subjects :
- Acute Disease
Biomarkers blood
Colorectal Neoplasms blood
Colorectal Neoplasms immunology
DNA, Complementary
Esophageal Neoplasms immunology
Esophageal Squamous Cell Carcinoma immunology
Humans
Immunoenzyme Techniques
Ischemic Stroke immunology
Neoplasm Proteins immunology
Esophageal Neoplasms blood
Esophageal Squamous Cell Carcinoma blood
Immunoglobulin G blood
Ischemic Stroke blood
LDL-Receptor Related Protein-Associated Protein immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1349-7006
- Volume :
- 111
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cancer science
- Publication Type :
- Academic Journal
- Accession number :
- 32939876
- Full Text :
- https://doi.org/10.1111/cas.14652