231 results on '"Zabel, Peter"'
Search Results
2. Linking pre-existing biorepositories for medical research: the PopGen 2.0 Network
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Lieb, Wolfgang, Jacobs, Gunnar, Wolf, Andreas, Richter, Gesine, Gaede, Karoline I., Schwarz, Jeanette, Arnold, Norbert, Böhm, Ruwen, Buyx, Alena, Cascorbi, Ingolf, Franke, Andre, Glinicke, Christine, Held-Feindt, Janka, Junker, Ralf, Kalthoff, Holger, Kramer, Hans-Heiner, Leypoldt, Frank, Maass, Nicolai, Maetzler, Walter, May, Sandra, Mehdorn, H. Maximilian, Röcken, Christoph, Schafmayer, Clemens, Schrappe, Martin, Schreiber, Stefan, Sebens, Susanne, Stephani, Ulrich, Synowitz, Michael, Weimer, Jörg, Zabel, Peter, Nöthlings, Ute, Röder, Christian, and Krawczak, Michael
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- 2019
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3. The association of cognitive functioning as measured by the DemTect with functional and clinical characteristics of COPD: results from the COSYCONET cohort
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von Siemens, Sarah Marietta, Perneczky, Robert, Waschki, Benjamin, Lutter, Johanna I, Welte, Tobias, Jörres, Rudolf A, Kahnert, Kathrin, group, COSYCONET study, Andreas, Stefan, Bals, Robert, Behr, Jürgen, Vogelmeier, Claus F, Bewig, Burkhard, Buhl, Roland, Ewert, Ralf, Stubbe, Beate, Gogol, Manfred, Grohé, Christian, Hauck, Rainer, Held, Matthias, Jany, Berthold, Henke, Markus, Herth, Felix, Höffken, Gerd, Katus, Hugo A, Kirsten, Anne-Marie, Watz, Henrik, Koczulla, Rembert, Kenn, Klaus, Kronsbein, Juliane, Kropf-Sanchen, Cornelia, Lange, Christoph, Kauffmann-Guerrero, Diego, Zabel, Peter, Pfeifer, Michael, Randerath, Winfried J, Seeger, Werner, Studnicka, Michael, Taube, Christian, Teschler, Helmut, Timmermann, Hartmut, Virchow, J Christian, Vogelmeier, Claus, Alter, Peter, Wagner, Ulrich, Wirtz, Hubert, Trudzinski, Franziska C, and Söhler, Sandra
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Male ,medicine.medical_specialty ,epidemiology [Cognitive Dysfunction] ,psychology [Pulmonary Disease, Chronic Obstructive] ,Medizin ,Comorbidity ,Cohort Studies ,03 medical and health sciences ,FEV1/FVC ratio ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Cognition ,epidemiology [Pulmonary Disease, Chronic Obstructive] ,Surveys and Questionnaires ,medicine ,Dementia ,Humans ,COPD ,Cognitive Dysfunction ,ddc:610 ,Cognitive skill ,Path analysis (statistics) ,Aged ,lcsh:RC705-779 ,business.industry ,Research ,physiology [Cognition] ,diagnosis [Pulmonary Disease, Chronic Obstructive] ,lcsh:Diseases of the respiratory system ,Middle Aged ,medicine.disease ,Mental Status and Dementia Tests ,humanities ,Cross-Sectional Studies ,Cognitive impairment ,diagnosis [Cognitive Dysfunction] ,030228 respiratory system ,Cohort ,Physical therapy ,Female ,psychology [Cognitive Dysfunction] ,business ,030217 neurology & neurosurgery ,Cognitive load - Abstract
Alterations of cognitive functions have been described in COPD. Our study aimed to disentangle the relationship between the degree of cognitive function and COPD characteristics including quality of life (QoL).Data from 1969 COPD patients of the COSYCONET cohort (GOLD grades 1–4; 1216 male/ 753 female; mean (SD) age 64.9 ± 8.4 years) were analysed using regression and path analysis. The DemTect screening tool was used to measure cognitive function, and the St. George‘s respiratory questionnaire (SGRQ) to assess disease-specific QoL.DemTect scores were =60 years of age. For statistical reasons, we used the average of both algorithms independent of age in all subsequent analyses. The DemTect scores were associated with oxygen content, 6-min-walking distance (6-MWD), C-reactive protein (CRP), modified Medical Research Council dyspnoea scale (mMRC) and the SGRQ impact score. Conversely, the SGRQ impact score was independently associated with 6-MWD, FVC, mMRC and DemTect. These results were combined into a path analysis model to account for direct and indirect effects. The DemTect score had a small, but independent impact on QoL, irrespective of the inclusion of COPD-specific influencing factors or a diagnosis of cognitive impairment.We conclude that in patients with stable COPD lower oxygen content of blood as a measure of peripheral oxygen supply, lower exercise capacity in terms of 6-MWD, and higher CRP levels were associated with reduced cognitive capacity. Furthermore, a reduction in cognitive capacity was associated with reduced disease-specific quality of life. As a potential clinical implication of this work, we suggest to screen especially patients with low oxygen content and low 6-MWD for cognitive impairment.
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- 2022
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4. The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique
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Marwitz, Sebastian, Kolarova, Julia, Reck, Martin, Reinmuth, Niels, Kugler, Christian, Schädlich, Ines, Haake, Andrea, Zabel, Peter, Vollmer, Ekkehard, Siebert, Reiner, Goldmann, Torsten, and Ammerpohl, Ole
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- 2014
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5. HOPE-preservation of paraffin-embedded sputum samples–A new way of bioprofiling in COPD
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Pedersen, Frauke, Marwitz, Sebastian, Seehase, Sophie, Kirsten, Anne-Marie, Zabel, Peter, Vollmer, Ekkehard, Rabe, Klaus F., Magnussen, Helgo, Watz, Henrik, and Goldmann, Torsten
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- 2013
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6. Interfering with the production of cytokines in sepsis
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Zabel, Peter, Bahrami, Soheyl, Parnham, Michael J., editor, Redl, Heinz, editor, and Schlag, Günther, editor
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- 1999
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7. Associations of oxygenated hemoglobin with disease burden and prognosis in stable COPD: Results from COSYCONET
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Trudzinski, F.C., Jörres, R.A., Alter, P., Kahnert, K., Waschki, B., Herr, C., Kellerer, C., Omlor, A., Vogelmeier, C.F., Fähndrich, S., Watz, H., Welte, T., Jany, B., Söhler, S., Biertz, F., Herth, F., Kauczor, H.-U., Bals, R., Andreas, Stefan, Behr, Jürgen, Bewig, Burkhard, Buhl, Roland, Ewert, Ralf, Stubbe, Beate, Ficker, Joachim H., Gogol, Manfred, Grohé, Christian, Hauck, Rainer, Held, Matthias, Henke, Markus, Höffken, Gerd, Katus, Hugo A., Kirsten, Anne-Marie, Koczulla, Rembert, Kenn, Klaus, Kronsbein, Juliane, Kropf-Sanchen, Lange, Christoph, Zabel, Peter, Pfeifer, Michael, Randerath, Winfried J., Seeger, Werner, Studnicka, Michael, Taube, Christian, Teschler, Helmut, Timmermann, Hartmut, Virchow, J. Christian, Wagner, and Wirtz, Hubert
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Adult ,Male ,medicine.medical_specialty ,Exacerbation ,Medizin ,lcsh:Medicine ,Severity of Illness Index ,Gastroenterology ,Article ,Pulmonary Disease, Chronic Obstructive ,03 medical and health sciences ,Medical research ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Signs and symptoms ,lcsh:Science ,Survival rate ,Aged ,Oxygen saturation (medicine) ,Aged, 80 and over ,Inflammation ,COPD ,Oxygenated Hemoglobin ,Multidisciplinary ,Proportional hazards model ,business.industry ,Hazard ratio ,lcsh:R ,Middle Aged ,Prognosis ,medicine.disease ,Comorbidity ,Survival Rate ,Risk factors ,030228 respiratory system ,Oxyhemoglobins ,Female ,lcsh:Q ,Blood Gas Analysis ,business ,Biomarkers - Abstract
We studied whether in patients with stable COPD blood gases (BG), especially oxygenated hemoglobin (OxyHem) as a novel biomarker confer information on disease burden and prognosis and how this adds to the information provided by the comorbidity pattern and systemic inflammation. Data from 2137 patients (GOLD grades 1–4) of the baseline dataset of the COSYCONET COPD cohort were used. The associations with dyspnea, exacerbation history, BODE-Index (cut-off ≤2) and all-cause mortality over 3 years of follow-up were determined by logistic and Cox regression analyses, with sex, age, BMI and pack years as covariates. Predictive values were evaluated by ROC curves. Capillary blood gases included SaO2, PaO2, PaCO2, pH, BE and the concentration of OxyHem [haemoglobin (Hb) x fractional SaO2, g/dL] as a simple-to-measure correlate of oxygen content. Inflammatory markers were WBC, CRP, IL-6 and -8, TNF-alpha and fibrinogen, and comorbidities comprised a broad panel including cardiac and metabolic disorders. Among BG, OxyHem was associated with dyspnoea, exacerbation history, BODE-Index and mortality. Among inflammatory markers and comorbidities, only WBC and heart failure were consistently related to all outcomes. ROC analyses indicated that OxyHem provided information of a magnitude comparable to that of WBC, with optimal cut-off values of 12.5 g/dL and 8000/µL, respectively. Regarding mortality, OxyHem also carried independent, additional information, showing a hazard ratio of 2.77 (95% CI: 1.85–4.15, p 8000/µL was 2.33 (95% CI: 1.60–3.39, p 2. It thus appears well suited for clinical use with minimal equipment, especially for GPs.
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- 2020
8. Dexamethasone and N-acetyl-cysteine attenuate Pseudomonas aeruginosa-induced mucus expression in human airways
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Sprenger, Lisa, Goldmann, Torsten, Vollmer, Ekkehard, Steffen, Armin, Wollenberg, Barbara, Zabel, Peter, and Hauber, Hans-Peter
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- 2011
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9. Budesonide Inhibits Intracellular Infection with Non-Typeable Haemophilus influenzae despite Its Anti-Inflammatory Effects in Respiratory Cells and Human Lung Tissue: A Role for p38 MAP Kinase
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Wagner, Christopher, Goldmann, Torsten, Rohmann, Kristina, Rupp, Jan, Marwitz, Sebastian, Rotta detto Loria, Johannes, Limmer, Stefan, Zabel, Peter, Dalhoff, Klaus, and Drömann, Daniel
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- 2015
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10. Quantitation of major protein constituents of murine intestinal fluid
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Bade, Steffen, Gorris, Hans H., Koelling, Sabine, Olivier, Verena, Reuter, Fabian, Zabel, Peter, and Frey, Andreas
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- 2010
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11. Continuous positive airway pressure therapy decreases evening cortisol concentrations in patients with severe obstructive sleep apnea
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Schmoller, Andre, Eberhardt, Frank, Jauch-Chara, Kamila, Schweiger, Ulrich, Zabel, Peter, Peters, Achim, Schultes, Bernd, and Oltmanns, Kerstin M.
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- 2009
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12. A model of the isolated perfused rat small intestine
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Lautenschlager, Ingmar, Dombrowsky, Heike, Frerichs, Inez, Kuchenbecker, Solveig-Carolin, Bade, Steffen, Schultz, Holger, Zabel, Peter, Scholz, Jens, Weiler, Norbert, and Uhlig, Stefan
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Intestine, Small -- Physiological aspects ,Intestine, Small -- Research ,Platelet activating factor -- Physiological aspects ,Biological sciences - Abstract
Intestinal edema remains a serious clinical problem, and novel approaches to study its pathophysiology are needed. It was our aim to develop a long-term stable isolated perfused rat small bowel preparation permitting analysis of vascular, luminal, interstitial, and lymphatic compartments and to demonstrate the utility of this model by studying the effects of the proinflammatory mediator platelet-activating factor (PAF). A temperature-controlled chamber with an integrated balance was designed to perfuse isolated intestines through the mesenteric artery and the gut lumen. Steroids or oxygen carriers were not needed. Functional and morphological integrity of the tissue was preserved for several hours as confirmed by oxygen consumption, venous lactate-to-pyruvate ratio, arterial and venous pH, lactose digestion and galactose uptake, intravascular and luminal pressures, maintained fluid homeostasis, gut motility, and quantitative light microscopic analysis. Administration of PAF caused typical effects such as vasoconstriction, gut atony, and loss of galactose uptake. PAF also elicited a transient loss of 20% of the perfusate liquid from the mesenteric vascular bed, two-thirds of which were transferred to the lumen. All these responses were entirely reversible. This new model provides detailed insights into the physiology of the small intestine and will allow to study fundamental processes such as fluid homeostasis, barrier functions, transport mechanisms, and immune responses in this organ. Using this model, here we show a dramatic and yet reversible response of the rat small bowel to PAF, suggesting luminal water clearance as a novel safety factor in the intestine that may be of clinical relevance. intestine physiology; fluid balance; platelet-activating factor doi: 10.1152/ajpgi.00313.2009
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- 2010
13. Relationship between clinical and radiological signs of bronchiectasis in COPD patients: Results from COSYCONET
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Kirsten Anne-Marie, Anne Wirz, Erich Traugott, Ficker Joachim H, Bertram J. Jobst, Vivien Janke, Stubbe Beate, Johanna I. Lutter, Barbara Ziss, Franziska C. Trudzinski, Patricia Berger, Henrik Watz, Gogol Manfred, Thomas Bahmer, Beate Polte, Kronsbein Juliane, Campus Kiel, Lange Christoph, Martina Seibert, Rudolf A. Jörres, Pfeifer Michael, Timmermann Hartmut, Grohé Christian, Tobias Welte, Studnicka Michael, Petra Hundack-Winter, Jana Graf, Jürgen Behr, Diana Schottel, Buhl Roland, Virchow J. Christian, Bewig Burkhard, Ruhrlandklinik gGmbH. Essen, Wirtz Hubert, Rosalie Untsch, Birte Struck, Peter Alter, Kathrin Kahnert, Gudrun Hübner, Vogelmeier Claus, Sabine Michalewski, Kropf-Sanchen Cornelia, Kenn Klaus, Pontus Mertsch, Sonja Rohweder, Hauck Rainer, Andreas Stefan, Ilona Kietzmann, Zabel Peter, Michaela Schrade-Illmann, Höffken Gerd, Julia Tobias, Frank Biertz, Seeger Werner, Manuel Klöser, Kahnert Kathrin, Teschler Helmut, Anita Reichel, Gina Spangel, Ulrike Rieber, Randerath Winfried J, Julia Teng, Tanja Lucke, Herth Felix, Jeanette Pieper, Lenka Krabbe, Taube Christian, Jürgen Biederer, Wagner Ulrich, Doris Lehnert, Claus Vogelmeier, Katrin Schwedler, Henke Markus, Jany Berthold, Katus Hugo A, Bals Robert, Zaklina Hinz, Cornelia Böckmann, Ellen Burmann, Margret Gleiniger, Behr Jürgen, Britta Markworth, Ewert Ralf, Gertraud Weiß, Katrin Wons, Barbara Arikan, Watz Henrik, Beate Schaufler, Lena Sterk, Robert Bals, Hans-Ulrich Kauczor, Koczulla Rembert, Held Matthias, and Welte Tobias
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Pulmonary and Respiratory Medicine ,Male ,medicine.medical_specialty ,Copd patients ,Medizin ,Comorbidity ,Severity of Illness Index ,Pulmonary Disease, Chronic Obstructive ,Medicine ,Humans ,In patient ,Lung ,Aged ,Aged, 80 and over ,COPD ,Bronchiectasis ,business.industry ,Phlegm ,Middle Aged ,medicine.disease ,Radiological weapon ,Clinical diagnosis ,Cohort ,Female ,Radiography, Thoracic ,Radiology ,medicine.symptom ,business ,Tomography, X-Ray Computed - Abstract
Bronchiectasis (BE) might be frequently present in COPD but masked by COPD symptoms. We studied the relationship of clinical signs of bronchiectasis to the presence and extent of its radiological signs in patients of different COPD severity. Visit 4 data (GOLD grades 1-4) of the COSYCONET cohort was used. Chest CT scans were evaluated for bronchiectasis in 6 lobes using a 3-point scale (0: absence, 1: ≤50%, 2: >50% BE-involvement for each lobe). 1176 patients were included (61%male, age 67.3y), among them 38 (3.2%) with reported physicians' diagnosis of bronchiectasis and 76 (6.5%) with alpha1-antitrypsin deficiency (AA1D). CT scans were obtained in 429 patients. Within this group, any signs of bronchiectasis were found in 46.6% of patients, whereby ≤50% BE occurred in 18.6% in ≤2 lobes, in 10.0% in 3-4 lobes, in 15.9% in 5-6 lobes; >50% bronchiectasis in at least 1 lobe was observed in 2.1%. Scores ≥4 correlated with an elevated ratio FRC/RV. The clinical diagnosis of bronchiectasis correlated with phlegm and cough and with radiological scores of at least 3, optimally ≥5. In COPD patients, clinical diagnosis and radiological signs of BE showed only weak correlations. Correlations became significant with increasing BE-severity implying radiological alterations in several lobes. This indicates the importance of reporting both presence and extent of bronchiectasis on CT. Further research is warranted to refine the criteria for CT scoring of bronchiectasis and to determine the relevance of radiologically but not clinically detectible bronchiectasis and their possible implications for therapy in COPD patients.
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- 2020
14. CAT score single item analysis in patients with COPD: results from COSYCONET
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J. Randerath Winfried, Pfeifer Michael, Kenn Klaus, Joachim H. Ficker, Gogol Manfred, Grohé Christian, Höffken Gerd, Zaklina Hinz, Julia Tobias, Henke Markus, Teschler Helmut, Welte Tobias, Benjamin Waschki, Buhl Roland, Paul W. Jones, Kirsten Anne-Marie, A. Katus Hugo, Taube Christian, Bewig Burkhard, Beate Polte, Kronsbein Juliane, Stubbe Beate, Bals Robert, Johanna I. Lutter, Sarah Marietta von Siemens, Lange Christoph, Vogelmeier Claus, Ellen Burmann, Wirtz Hubert, Kathrin Kahnert, Erich Traugott, Behr Jürgen, Birte Struck, Vivien Janke, Lenka Krabbe, Timmermann Hartmut, Wagner Ulrich, Anita Reichel, Sabine Michalewski, Gudrun Hübner, Seeger Werner, Doris Lehnert, Jany Berthold, Kropf-Sanchen Cornelia, Sandra Söhler, Jeanette Pieper, Ulrike Rieber, Peter Alter, Herth Felix, Zabel Peter, Andreas Stefan, Koczulla Rembert, Held Matthias, Tobias Welte, Franziska C. Trudzinski, Patricia Berger, Kahnert Kathrin, Jana Graf, Jürgen Behr, Rosalie Untsch, Rudolf A. Jörres, Kornelia Speth, Britta Markworth, Ewert Ralf, Gertraud Weiß, Hans-Ulrich Kauczor, Claus Vogelmeier, Katrin Schwedler, Katrin Wons, Bertram J. Jobst, Barbara Arikan, Margret Gleiniger, Henrik Watz, Watz Henrik, Studnicka Michael, Beate Schaufler, Diana Schottel, Sonja Rohweder, Robert Bals, Ilona Kietzmann, Virchow J. Christian, Burkhard Bewig, Hauck Rainer, and Michaela Schrade-Illmann
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Pulmonary and Respiratory Medicine ,Percentile ,medicine.medical_specialty ,Medizin ,Diagnostic Techniques, Respiratory System ,Single item ,CAT score ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Internal medicine ,medicine ,COPD ,In patient ,030212 general & internal medicine ,Lung function ,Emphysema ,business.industry ,Regression analysis ,Cat Score ,Copd ,medicine.disease ,Exploratory factor analysis ,respiratory tract diseases ,030228 respiratory system ,Cohort ,business - Abstract
The COPD Assessment Test (CAT) is in widespread use for the evaluation of patients with chronic obstructive pulmonary disease (COPD). We assessed whether the CAT items carry additional information beyond the sum score regarding COPD characteristics including emphysema. Patients of GOLD grades 1 to 4 from the COPD cohort COSYCONET (German COPD and Systemic Consequences - Comorbidities Network) with complete CAT data were included (n = 2270), of whom 493 had chest CT evaluated for the presence of emphysema. Comorbidities and lung function were assessed following standardised procedures. Cross-sectional data analysis was based on multiple regression analysis of the single CAT items against a panel of comorbidities, lung function, or CT characteristics (qualitative score, 15th percentile of mean lung density), with age, BMI and gender as covariates. This was supported by exploratory factor analysis. Regarding the relationship to comorbidities and emphysema, there were marked differences between CAT items, especially items 1 and 2 versus 3 to 8. This grouping was basically confirmed by factor analysis. Items 4 and 5, and to a lower degree 1, 2 and 6, appeared to be informative regarding the presence of emphysema, whereas the total score was not or less informative. Regarding comorbidities, similar findings as for the total CAT score were obtained for the modified Medical Research Council scale (mMRC) which was also informative regarding emphysema. Our findings suggest that the usefulness of the CAT can be increased if evaluated on the basis of single items which may be indicating the presence of comorbidities and emphysema.
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- 2020
15. Treatment and vaccines for severe acute respiratory syndrome
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Groneberg, David A., Poutanen, Susan M., Low, Donald E., Lode, Hartmut, Welte, Tobias, and Zabel, Peter
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Medical colleges -- Analysis ,Medical colleges -- Health aspects ,Severe acute respiratory syndrome -- Development and progression ,Severe acute respiratory syndrome -- Analysis ,Severe acute respiratory syndrome -- Health aspects ,Vaccines -- Analysis ,Vaccines -- Health aspects - Published
- 2005
16. Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung
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Droemann, Daniel, Goldmann, Torsten, Branscheid, Detlev, Clark, Ryan, Dalhoff, Klaus, Zabel, Peter, and Vollmer, Ekkehard
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- 2003
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17. The Effect of Dithiothreitol on the Transcriptome of Induced Sputum Cells
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Goldmann, Torsten, Pedersen, Frauke, Seehase, Sophie, Marwitz, Sebastian, Lang, Dagmar S., Kirsten, Anne-Marie, Zabel, Peter, Vollmer, Ekkehard, Magnussen, Helgo, Rabe, Klaus F., and Watz, Henrik
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- 2013
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18. Vasoproliferation and Antiproliferative Treatment Options in Pulmonary Arterial Hypertension
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Blaukovitsch, Markus, Zabel, Peter, and Hauber, Hans-Peter
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- 2009
19. The interleukin-6 −174 promoter polymorphism is associated with extrapulmonary bacterial dissemination in Streptococcus pneumoniae infection
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Schaaf, Bernhard, Rupp, Jan, Müller-Steinhardt, Michael, Kruse, Jan, Boehmke, Florian, Maass, Matthias, Zabel, Peter, and Dalhoff, Klaus
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- 2005
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20. Modulation of the Inflammatory Response to Streptococcus pneumoniae in a Model of Acute Lung Tissue Infection
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Xu, Feng, Droemann, Daniel, Rupp, Jan, Shen, Huahao, Wu, Xiaohong, Goldmann, Torsten, Hippenstiel, Stefan, Zabel, Peter, and Dalhoff, Klaus
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- 2008
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21. Combined effects of lung function, blood gases and kidney function on the exacerbation risk in stable COPD: Results from the COSYCONET cohort
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F.C. Trudzinski, K. Kahnert, C.F. Vogelmeier, P. Alter, F. Seiler, S. Fähndrich, H. Watz, T. Welte, T. Speer, S. Zewinger, F. Biertz, H.-U. Kauczor, R.A. Jörres, R. Bals, Andreas Stefan, Bals Robert, Behr Jürgen, Kahnert Kathrin, Bewig Burkhard, Buhl Roland, Ewert Ralf, Stubbe Beate, Ficker Joachim H, Gogol Manfred, Grohé Christian, Hauck Rainer, Held Matthias, Jany Berthold, Henke Markus, Herth Felix, Höffken Gerd, Katus Hugo A, Kirsten Anne-Marie, Watz Henrik, Koczulla Rembert, Kenn Klaus, Kronsbein Juliane, Kropf-Sanchen Cornelia, Lange Christoph, Zabel Peter, Pfeifer Michael, Randerath Winfried J, null eeger Werner, Studnicka Michael, Taube Christian, Teschler Helmut, Timmermann Hartmut, Virchow J. Christian, Vogelmeier Claus, Wagner Ulrich, Welte Tobias, and Wirtz Hubert
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Pulmonary and Respiratory Medicine ,Male ,medicine.medical_specialty ,Exacerbation ,Partial Pressure ,Medizin ,Renal function ,Comorbidity ,Acid-Base Imbalance ,Kidney Function Tests ,Risk Assessment ,Cohort Studies ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,DLCO ,Diffusing capacity ,Internal medicine ,Forced Expiratory Volume ,medicine ,Humans ,Respiratory function ,030212 general & internal medicine ,Aged ,COPD ,Carbon Monoxide ,business.industry ,Middle Aged ,medicine.disease ,respiratory tract diseases ,Respiratory Function Tests ,Cross-Sectional Studies ,030228 respiratory system ,Cohort ,Cardiology ,Disease Progression ,Pulmonary Diffusing Capacity ,Female ,Blood Gas Analysis ,Risk assessment ,business ,Glomerular Filtration Rate - Abstract
Rationale Alterations of acid-base metabolism are an important outcome predictor in acute exacerbations of COPD, whereas sufficient metabolic compensation and adequate renal function are associated with decreased mortality. In stable COPD there is, however, only limited information on the combined role of acid-base balance, blood gases, renal and respiratory function on exacerbation risk grading. Methods We used baseline data of the COPD cohort COSYCONET, applying linear and logistic regression analyses, the results of which were implemented into a comprehensive structural equation model. As most informative parameters it comprised the estimated glomerular filtration rate (eGFR), lung function defined via forced expiratory volume in 1 s (FEV1), intrathoracic gas volume (ITGV) and (diffusing capacity for carbon monoxide (DLCO), moreover arterial oxygen content (CaO2), partial pressure of oxygen (PaCO2), base exess (BE) and exacerbation risk according to GOLD criteria. All measures were adjusted for age, gender, body-mass index, the current smoking status and pack years. Results 1506 patients with stable COPD (GOLD grade 1–4; mean age 64.5 ± 8.1 y; mean FEV1 54 ± 18 %predicted, mean eGFR 82.3 ± 16.9 mL/min/1.73 m2) were included. BE was linked to eGFR, lung function and PaCO2 and played a role as indirect predictor of exacerbation risk via these measures; moreover, eGFR was directly linked to exacerbation risk. These associations remained significant after taking into account medication (diuretics, oral and inhaled corticosteroids), whereby corticosteroids had effects on exacerbation risk and lung function, diuretics on eGFR, BE and lung function. Conclusion Even in stable COPD acid-base metabolism plays a key integrative role in COPD risk assessment despite rather small deviations from normality. It partially mediates the effects of impairments in kidney function, which are also directly linked to exacerbation risk.
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- 2019
22. Effect of Dexamethasone and ACC on Bacteria-Induced Mucin Expression in Human Airway Mucosa
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Hauber, Hans-Peter, Goldmann, Torsten, Vollmer, Ekkehard, Wollenberg, Barbara, and Zabel, Peter
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- 2007
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23. Disparate Innate Immune Responses to Persistent and Acute Chlamydia pneumoniae Infection in Chronic Obstructive Pulmonary Disease
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Droemann, Daniel, Rupp, Jan, Goldmann, Torsten, Uhlig, Ulrike, Branscheid, Detlev, Vollmer, Ekkehard, Kujath, Peter, Zabel, Peter, and Dalhoff, Klaus
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- 2007
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24. Pneumococcal Septic Shock Is Associated with the Interleukin-10-1082 Gene Promoter Polymorphism
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Schaaf, Bernhard M., Boehmke, Florian, Esnaashari, Hamed, Seitzer, Ulrike, Kothe, Henning, Maass, Matthias, Zabel, Peter, and Dalhoff, Klaus
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- 2003
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25. Tumor Necrosis Factor-α - 308 Promoter Gene Polymorphism and Increased Tumor Necrosis Factor Serum Bioactivity in Farmerʼs Lung Patients
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SCHAAF, BERNHARD M., SEITZER, ULRIKE, PRAVICA, VERA, ARIES, SVEN P., and ZABEL, PETER
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- 2001
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26. Correction: effect of low tidal ventilation on lung function and inflammation in mice
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Hauber Hans P, Karp Dörte, Goldmann Torsten, Vollmer Ekkehard, and Zabel Peter
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Diseases of the respiratory system ,RC705-779 - Published
- 2012
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27. Gender Differences in Human Sepsis
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Schroder, Jorg, Kahlke, Volker, Staubach, Karl-Hermann, Zabel, Peter, and Stuber, Frank
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- 1998
28. The TGF-beta-Pseudoreceptor BAMBI is strongly expressed in COPD lungs and regulated by nontypeable Haemophilus influenzae
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Schultz Holger, Abdullah Mahdi, Röschmann Kristina, Marwitz Sebastian, Ulmer Artur J, Osbahr Sinia, Rohmann Kristina, Rupp Jan, Drömann Daniel, Vollmer Ekkehard, Zabel Peter, Dalhoff Klaus, and Goldmann Torsten
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Nontypeable Haemophilus influenzae (NTHI) may play a role as an infectious trigger in the pathogenesis of chronic obstructive pulmonary disease (COPD). Few data are available regarding the influence of acute and persistent infection on tissue remodelling and repair factors such as transforming growth factor (TGF)-β. Methods NTHI infection in lung tissues obtained from COPD patients and controls was studied in vivo and using an in vitro model. Infection experiments were performed with two different clinical isolates. Detection of NTHI was done using in situ hybridization (ISH) in unstimulated and in in vitro infected lung tissue. For characterization of TGF-β signaling molecules a transcriptome array was performed. Expression of the TGF-pseudoreceptor BMP and Activin Membrane-bound Inhibitor (BAMBI) was analyzed using immunohistochemistry (IHC), ISH and PCR. CXC chemokine ligand (CXCL)-8, tumor necrosis factor (TNF)-α and TGF-β expression were evaluated in lung tissue and cell culture using ELISA. Results In 38% of COPD patients infection with NTHI was detected in vivo in contrast to 0% of controls (p < 0.05). Transcriptome arrays showed no significant changes of TGF-β receptors 1 and 2 and Smad-3 expression, whereas a strong expression of BAMBI with upregulation after in vitro infection of COPD lung tissue was demonstrated. BAMBI was expressed ubiquitously on alveolar macrophages (AM) and to a lesser degree on alveolar epithelial cells (AEC). Measurement of cytokine concentrations in lung tissue supernatants revealed a decreased expression of TGF-β (p < 0.05) in combination with a strong proinflammatory response (p < 0.01). Conclusions We show for the first time the expression of the TGF pseudoreceptor BAMBI in the human lung, which is upregulated in response to NTHI infection in COPD lung tissue in vivo and in vitro. The combination of NTHI-mediated induction of proinflammatory cytokines and inhibition of TGF-β expression may influence inflammation induced tissue remodeling.
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- 2010
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29. Effect of low tidal volume ventilation on lung function and inflammation in mice
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Goldmann Torsten, Karp Dörte, Hauber Hans P, Vollmer Ekkehard, and Zabel Peter
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background A large number of studies have investigated the effects of high tidal volume ventilation in mouse models. In contrast data on very short term effects of low tidal volume ventilation are sparse. Therefore we investigated the functional and structural effects of low tidal volume ventilation in mice. Methods 38 Male C57/Bl6 mice were ventilated with different tidal volumes (Vt 5, 7, and 10 ml/kg) without or with application of PEEP (2 cm H2O). Four spontaneously breathing animals served as controls. Oxygen saturation and pulse rate were monitored. Lung function was measured every 5 min for at least 30 min. Afterwards lungs were removed and histological sections were stained for measurement of infiltration with polymorphonuclear leukocytes (PMN). Moreover, mRNA expression of macrophage inflammatory protein (MIP)-2 and tumor necrosis factor (TNF)α in the lungs was quantified using real time PCR. Results Oxygen saturation did not change significantly over time of ventilation in all groups (P > 0.05). Pulse rate dropped in all groups without PEEP during mechanical ventilation. In contrast, in the groups with PEEP pulse rate increased over time. These effects were not statistically significant (P > 0.05). Tissue damping (G) and tissue elastance (H) were significantly increased in all groups after 30 min of ventilation (P < 0.05). Only the group with a Vt of 10 ml/kg and PEEP did not show a significant increase in H (P > 0.05). Mechanical ventilation significantly increased infiltration of the lungs with PMN (P < 0.05). Expression of MIP-2 was significantly induced by mechanical ventilation in all groups (P < 0.05). MIP-2 mRNA expression was lowest in the group with a Vt of 10 ml/kg + PEEP. Conclusions Our data show that very short term mechanical ventilation with lower tidal volumes than 10 ml/kg did not reduce inflammation additionally. Formation of atelectasis and inadequate oxygenation with very low tidal volumes may be important factors. Application of PEEP attenuated inflammation.
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- 2010
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30. Comparison of the effect of lps and pam3 on ventilated lungs
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Goldmann Torsten, Karp Dörte, Hauber Hans P, Vollmer Ekkehard, and Zabel Peter
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background While lipopolysaccharide (LPS) from Gram-negative bacteria has been shown to augment inflammation in ventilated lungs information on the effect of Gram-positive bacteria is lacking. Therefore the effect of LPS and a lipopetide from Gram-positive bacteria, PAM3, on ventilated lungs were investigated. Methods C57/Bl6 mice were mechanically ventilated. Sterile saline (sham) and different concentrations of LPS (1 μg and 5 μg) and PAM3 (50 nM and 200 nM) were applied intratracheally. Lung function parameters and expression of MIP-2 and TNFα as well as influx of neutrophils were measured. Results Mechanical ventilation increased resistance and decreased compliance over time. PAM3 but not LPS significantly increased resistance compared to sham challenge (P < 0.05). Both LPS and PAM3 significantly increased MIP-2 and TNFα mRNA expression compared to sham challenge (P < 0.05). The numbers of neutrophils were significantly increased after LPS at a concentration of 5 μg compared to sham (P < 0.05). PAM3 significantly increased the numbers of neutrophils at both concentrations compared to sham (P < 0.05). Conclusions These data suggest that PAM3 similar to LPS enhances ventilator-induced inflammation. Moreover, PAM3 but not LPS increases pulmonary resistance in ventilated lungs. Further studies are warranted to define the role of lipopetides in ventilator-associated lung injury.
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- 2010
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31. Serum Level of Interleukin 8 Is Elevated in Idiopathic Pulmonary Fibrosis and Indicates Disease Activity
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ZIEGENHAGEN, MANFRED W., ZABEL, PETER, ZISSEL, GERNOT, SCHLAAK, MAX, and MÜLLER-QUERNHEIM, JOACHIM
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- 1998
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32. Effect of Pentoxifylline in Severe Sepsis: Results of a Randomized, Double-blind, Placebo-Controlled Study
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Staubach, Karl-Hermann, Schroder, Jorg, Stuber, Frank, Gehrke, Katrin, Traumann, Emanuel, and Zabel, Peter
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- 1998
33. Sarcoidosis: TNF-α Release from Alveolar Macrophages and Serum Level of sIL-2R Are Prognostic Markers
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ZIEGENHAGEN, MANFRED W., BENNER, UDO K., ZISSEL, GERNOT, ZABEL, PETER, SCHLAAK, MAX, and MÜLLER-QUERNHEIM, JOACHIM
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- 1997
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34. The human placenta releases substances that drive lung cancer into apoptosis
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Zabel Peter, Hauber Hans-Peter, Abdullah Mahdi, Kähler Daniel, Schultz Holger, Zeiser Tobias, Marwitz Sebastian, Vollmer Ekkehard, and Goldmann Torsten
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Pathology ,RB1-214 - Abstract
Abstract Background As there is no optimal treatment of non small cell lung cancer due to its resistance to common chemotherapeutics, we investigated the effect of human placenta-conditioned medium on tumor tissue. The human placenta constitutes a mixture of maternal and fetal origin and displays a variety of immunomodulatory aspects. Methods Freshly resected non small cell lung cancer tissues were incubated with placenta-conditioned medium in a short-term tissue culture model and A549 cells were challenged, respectively. Term placenta was used for producing conditioned medium and HOPE-fixed stimulated tumor tissue was analyzed for expression of caspase-3 and Ki67 via immunohistochemistry. The effects of conditioned medium on squamous cell carcinoma were further compared to physiological concentrations of Carboplat/Gemzar. Results Conditioned medium caused in 2 of 3 cases elevated expression of caspase-3 and reduced expression of Ki67 in 3 out of 3 cases, while the chemotherapeutic agents caused no comparable expression of caspase-3 or reduction of Ki67. In cell culture up to 50% of karyopyknosis was investigated and even sterile-filtrated medium caused widespread reduction of Ki67 on protein level. Conclusion Human placenta releases substances that mediate apoptosis and reduce proliferation in tumor tissue and cell culture. As even sterile-filtrated medium caused the mentioned effects we hypothesize one or more soluble mediators. The detailed way of promoting apoptosis and nature of these mediators need to be elucidated in further studies.
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- 2009
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35. PAS staining of bronchoalveolar lavage cells for differential diagnosis of interstital lung disease
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Zabel Peter and Hauber Hans P
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Pathology ,RB1-214 - Abstract
Abstract Bronchoalveolar lavage (BAL) is a useful diagnostic tool in interstitial lunge diseases (ILD). However, differential cell counts are often non specific and immunocytochemistry is time consuming. Staining of glyoproteins by periodic acid Schiff (PAS) reaction may help in discriminating different forms of ILD. In addition, PAS staining is easy to perform. BAL cells from patients with idiopathic pulmonary fibrosis (IPF) (n = 8), sarcoidosis (n = 9), and extrinsic allergic alveolitis (EAA) (n = 2) were investigated. Cytospins from BAL cells were made and cells were stained using Hemacolor quick stain and PAS staining. Lymphocytic alveolitis was found in sarcoidosis and EAA whereas in IPF both lymphocytes and neutrophils were increased. PAS positive cells were significantly decreased in EAA compared to IPF and sarcoidosis (25.5% ± 0.7% vs 59.8% ± 25.1% and 64.0% ± 19.7%, respectively) (P < 0.05). No significant correlation between PAS positive cells and inflammatory cells was observed. These results suggest that PAS staining of BAL cells may provide additional information in the differential diagnosis of ILD. Further studies ware warranted to evaluate PAS staining in larger numbers of BAL from patients with ILD.
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- 2009
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36. LED-FISH: Fluorescence microscopy based on light emitting diodes for the molecular analysis of Her-2/neu oncogene amplification
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Vollmer Ekkehard, Stellmacher Florian, Schultz Holger, Zeiser Tobias, Lang Dagmar S, Zabel Peter, and Goldmann Torsten
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Pathology ,RB1-214 - Abstract
Abstract Light emitting diodes (LED), which are available as small monochromatic light sources with characteristic features such as maximum illumination power combined with minimum energy consumption and extremely long lifespan have already proved as a highly potential low-cost alternative for specific diagnostic applications in clinical medicine such as tuberculosis fluorescence microscopy. Likewise, the most reliable evaluation of Her-2/neu (c-erbB2) gene amplification, which has been established in the last few years for routine diagnosis in clinical pathology as determinant towards Herceptin-based treatment of patients with breast cancer, is based on fluorescence in situ hybridization (FISH) and corresponding high priced fluorescence equipment. In order to test the possibility to utilize the advantages of low-cost LED technology on FISH analysis of c-erbB2 gene expression for routine diagnostic purposes, the applicability of a standard bright field Carl Zeiss Axiostar Plus microscope equipped with a Fraen AFTER* LED Fluorescence Microscope Kit for the detection of Her-2/neu gene signals was compared to an advanced Nikon Eclipse 80i fluorescence microscope in combination with a conventional 100W mercury vapor lamp. Both microscopes were fitted with the same Quicam FAST CCD digital camera to unequivocally compare the quality of the captured images. C-erbB2 gene expression was analyzed in 30 different human tissue samples of primary invasive breast cancer, following formalin fixation and subsequent paraffin-embedding. The Her2/neu gene signals (green) were identifiable in the tumor cells in all cases and images of equal quality were captured under almost identical conditions by 480 nm (blue) LED module equipped standard Axiostar microscope as compared to conventional fluorescence microscopy. In this first attempt, these monochromatic LED elements proved in principle to be suitable for the detection of Her-2/neu gene expression by FISH. Thus, our own experiences emphasize the high potential of this technology to provide a serious alternative to conventional fluorescence microscopy in routine pathology; representing a sustainable technological progress, this low-cost technology will clearly give direction also to the growing field of molecular pathology. * AFTER = Amplified Fluorescence by transmitted Excitation of Radiation
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- 2008
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37. TKTL1 is overexpressed in a large portion of non-small cell lung cancer specimens
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Zabel Peter, Vollmer Ekkehard, Branscheid Detlev, Kähler Daniel, Schultz Holger, and Goldmann Torsten
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Pathology ,RB1-214 - Abstract
Abstract In several tumors the transketolase activity, controlled inter alia by enzymes of the pentose phosphate pathway which is an alternative, energy generating reaction-cascade to glycolysis, has been correlated with proliferation. The increase of thiamine-dependant transketolase enzyme reactions is induced especially through upregulated transketolase-like enzyme 1 (TKTL1)-activity; that shows TKTL1 to be a causative enzyme for tumors enhanced, anaerobic glucose degradation. We investigated TKTL1-expression in 88 human, formalin-fixed non-small cell lung cancer tissues and 24 carcinomas of the breast by immunohistochemical stainings applying a 0 to 3 staining-score system (3 = strongest expression). For means of validation we additionally stained 40 NSCLC fixed and paraffin-embedded utilizing the HOPE-technique; showing comparable results to the formalin-fixed, paraffin-embedded specimens (not shown). Potential correlations with age, sex, TNM-classification parameters and tumor grading as well as tumor transcription factor 1 (TTF1) and surfactant protein A (SPA) expression were investigated. 40.9% of the analyzed lung tumors expressed TKTL1 weakly (Score 1), 38.6% moderately (score 2) and 17.1% strongly (score 3). 3 tumors were diagnosed TKTL1-negative (3.4%; score 0). All Breast cancer specimen stainings were positive and scored 1: 32%; scored 2: 36%; scored 3: 32%. Alveolar macrophages and Alveolar Epithelial Cells Type II were also found to be TKTL1-positive. None of the listed clinical parameters could be found to show a significant correlation to TKTL1 signal appearance. Although we describe the expression of TKTL1 in lung cancers, we need to state that up till now there is no scientific indication for any treatment regimens based upon these findings.
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- 2008
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38. A novel human ex vivo model for the analysis of molecular events during lung cancer chemotherapy
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Lang Dagmar S, Droemann Daniel, Schultz Holger, Branscheid Detlev, Martin Christian, Ressmeyer Anne R, Zabel Peter, Vollmer Ekkehard, and Goldmann Torsten
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background Non-small cell lung cancer (NSCLC) causes most of cancer related deaths in humans and is characterized by poor prognosis regarding efficiency of chemotherapeutical treatment and long-term survival of the patients. The purpose of the present study was the development of a human ex vivo tissue culture model and the analysis of the effects of conventional chemotherapy, which then can serve as a tool to test new chemotherapeutical regimens in NSCLC. Methods In a short-term tissue culture model designated STST (Short-Term Stimulation of Tissues) in combination with the novel *HOPE-fixation and paraffin embedding method we examined the responsiveness of 41 human NSCLC tissue specimens to the individual cytotoxic drugs carboplatin, vinorelbine or gemcitabine. Viability was analyzed by LIFE/DEAD assay, TUNEL-staining and colorimetric MTT assay. Expression of Ki-67 protein and of BrdU (bromodeoxyuridine) uptake as markers for proliferation and of cleaved (activated) effector caspase-3 as indicator of late phase apoptosis were assessed by immunohistochemistry. Transcription of caspase-3 was analyzed by RT-PCR. Flow cytometry was utilized to determine caspase-3 in human cancer cell lines. Results Viability, proliferation and apoptosis of the tissues were moderately affected by cultivation. In human breast cancer, small-cell lung cancer (SCLC) and human cell lines (CPC-N, HEK) proliferative capacity was clearly reduced by all 3 chemotherapeutic agents in a very similar manner. Cleavage of caspase-3 was induced in the chemo-sensitive types of cancer (breast cancer, SCLC). Drug-induced effects in human NSCLC tissues were less evident than in the chemo-sensitive tumors with more pronounced effects in adenocarcinomas as compared to squamous cell carcinomas. Conclusion Although there was high heterogeneity among the individual tumor tissue responses as expected, we clearly demonstrate specific multiple drug-induced effects simultaneously. Thus, STST provides a useful human model to study numerous aspects of mechanisms underlying tumor responsiveness towards improved anticancer treatment. The results presented here shall serve as a base for multiple functional tests of novel chemotherapeutic approaches to NSCLC in the future. *Hepes – Glutamic acid buffer mediated Organic solvent Protection Effect
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- 2007
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39. Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection
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Lang Dagmar, Loeschke Siegfried, Sauer Ulrich, Burgemeister Renate, Goldmann Torsten, Branscheid Detlev, Zabel Peter, and Vollmer Ekkehard
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Pathology ,RB1-214 - Abstract
Abstract As part of an investigation aimed at illuminating the possibilities and limits of the HOPE-fixation and paraffin-embedding technique we here describe a novel procedure which was developed in order to combine the benefits of the HOPE-technique with the capabilities of laser microdissection. The presented procedure avoids the need for amplification of template-RNA thus facilitating reliable and reproducible results. The excellent preservation of nucleic acids, proteins, and morphology in HOPE-fixed, paraffin-embedded tissues enhances the molecular applications available to date with materials acquired by laser microdissection when compared to formalin fixed, paraffin-embedded tissues, thus substantially extending the methodological panel in tissue based research.
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- 2006
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40. Toll-like receptor 2 expression is decreased on alveolar macrophages in cigarette smokers and COPD patients
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Zabel Peter, Tiedje Thorsten, Goldmann Torsten, Droemann Daniel, Dalhoff Klaus, and Schaaf Bernhard
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Backround Cigarette smoke exposure including biologically active lipopolysaccharide (LPS) in the particulate phase of cigarette smoke induces activation of alveolar macrophages (AM) and alveolar epithelial cells leading to production of inflammatory mediators. This represents a crucial mechanism in the pathogenesis of chronic obstructive pulmonary disease (COPD). Respiratory pathogens are a major cause of exacerbations leading to recurrent cycles of injury and repair. The interaction between pathogen-associated molecular patterns and the host is mediated by pattern recognition receptors (PRR's). In the present study we characterized the expression of Toll-like receptor (TLR)- 2, TLR4 and CD14 on human AM compared to autologous monocytes obtained from patients with COPD, healthy smokers and non-smokers. Methods The study population consisted of 14 COPD patients without evidence for acute exacerbation, 10 healthy smokers and 17 healthy non-smokers stratified according to age. The expression of TLR2, TLR4 and CD14 surface molecules on human AM compared to autologous monocytes was assessed ex vivo using FACS analysis. In situ hybridization was performed on bronchoalveolar lavage (BAL) cells by application of the new developed HOPE-fixative. Results The expression of TLR2, TLR4 and CD14 on AM from COPD patients, smokers and non-smokers was reduced as compared to autologous monocytes. Comparing AM we detected a reduced expression of TLR2 in COPD patients and smokers. In addition TLR2 mRNA and protein expression was increased after LPS stimulation on non-smokers AM in contrast to smokers and COPD patients. Conclusion Our data suggest a smoke related change in the phenotype of AM's and the cellular response to microbial stimulation which may be associated with impairment of host defenses in the lower respiratory tract.
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- 2005
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41. Molecular mechanisms of severe acute respiratory syndrome (SARS)
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Zabel Peter, Hilgenfeld Rolf, and Groneberg David A
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Severe Acute Respiratory Syndrome ,SARS ,coronavirus ,molecular mechanisms ,therapy ,vaccination ,Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Severe acute respiratory syndrome (SARS) is a new infectious disease caused by a novel coronavirus that leads to deleterious pulmonary pathological features. Due to its high morbidity and mortality and widespread occurrence, SARS has evolved as an important respiratory disease which may be encountered everywhere in the world. The virus was identified as the causative agent of SARS due to the efforts of a WHO-led laboratory network. The potential mutability of the SARS-CoV genome may lead to new SARS outbreaks and several regions of the viral genomes open reading frames have been identified which may contribute to the severe virulence of the virus. With regard to the pathogenesis of SARS, several mechanisms involving both direct effects on target cells and indirect effects via the immune system may exist. Vaccination would offer the most attractive approach to prevent new epidemics of SARS, but the development of vaccines is difficult due to missing data on the role of immune system-virus interactions and the potential mutability of the virus. Even in a situation of no new infections, SARS remains a major health hazard, as new epidemics may arise. Therefore, further experimental and clinical research is required to control the disease.
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- 2005
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42. Human lung cancer cells express functionally active Toll-like receptor 9
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Vollmer Ekkehard, Branscheid Detlev, Ulmer Artur J, Gerdes Johannes, Albrecht Dirk, Droemann Daniel, Dalhoff Klaus, Zabel Peter, and Goldmann Torsten
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Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background CpG-oligonucleotides (CpG-ODN), which induce signaling through Toll-like receptor 9 (TLR9), are currently under investigation as adjuvants in therapy against infections and cancer. CpG-ODN function as Th-1 adjuvants and are able to activate dendritic cells. In humans TLR9 has been described to be strongly expressed in B-lymphocytes, monocytes, plasmacytoid dendritic cells and at low levels in human respiratory cells. We determined whether a direct interaction of bacterial DNA with the tumor cells themselves is possible and investigated the expression and function of TLR9 in human malignant solid tumors and cell lines. TLR9 expression by malignant tumor cells, would affect treatment approaches using CpG-ODN on the one hand, and, on the other hand, provide additional novel information about the role of tumor cells in tumor-immunology. Methods The expression of TLR9 in HOPE-fixed non-small lung cancer, non-malignant tissue and tumor cell lines was assessed using immunohistochemistry, confocal microscopy, in situ hybridization, RT-PCR and DNA-sequencing. Apoptosis and chemokine expression was detected by FACS analysis and the Bio-Plex system. Results We found high TLR9 signal intensities in the cytoplasm of tumor cells in the majority of lung cancer specimens as well as in all tested tumor cell lines. In contrast to this non-malignant lung tissues showed only sporadically weak expression. Stimulation of HeLa and A549 cells with CpG-ODN induced secretion of monocyte chemoattractant protein-1 and reduction of spontaneous and tumor necrosis factor-alpha induced apoptosis. Conclusions Here we show that TLR9 is expressed in a selection of human lung cancer tissues and various tumor cell lines. The expression of functionally active TLR9 in human malignant tumors might affect treatment approaches using CpG-ODN and shows that malignant cells can be regarded as active players in tumor-immunology.
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- 2005
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43. Antibiotika-Forschung - 5 Jahre danach: Was hat sich getan, was bleibt zu tun?
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Deutsche Akademie Der Naturforscher Leopoldina, Akademie Der Wissenschaften (Hamburg), Addo, Marylyn, Breuer, Constanze, Fleischer, Bernhard, Gr��ger, Thomas, Hammann, Peter, Happe, Kathrin, Hecker, Michael, Lohse, Ansgar, Mettenleiter, Thomas, M��ller, Rolf, Rietschel, Ernst, R��bsamen-Schaeff, Helga, Sahl, Hans-Georg, Solbach, Werner, Wieler, Lothar, Zabel, Peter, and Senne, Elke
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Pharmazie ,Antibiotikaresistenz ,Immunologie ,Antibiotikum - Abstract
Einer stetig zunehmenden Zahl von Infektionen durch Antibiotika-resistente Bakterien stehen immer weniger neue Antibiotika gegen��ber - das ���Antibiotika-Problem���. Aus diesem Anlass ver��ffentlichten die Leopoldina und die Akademie der Wissenschaften in Hamburg im Jahr 2013 die Stellungnahme ���Antibiotika-Forschung: Probleme und Perspektiven���, die auf einen Workshop 2012 zur��ckging. Mit dem vorliegenden Papier weisen die Akademien nun ��� 5 Jahre nach dem ersten Workshop - auf eine Reihe von Punkten hin, welche aus Sicht der Wissenschaft bei der L��sung des Antibiotika-Problems nicht aus dem Blick geraten d��rfen. Es geht im Wesentlichen um zwei Aspekte: die in vielen L��ndern zunehmende Resistenz von bakteriellen Infektionserregern gegen die verf��gbaren Antibiotika und das Problem fehlender neuer Antibiotika., Diskussion, vol. 12, 2017
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- 2017
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44. Phenotypes of organ involvement in sarcoidosis
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Schupp, Jonas Christian, primary, Freitag-Wolf, Sandra, additional, Bargagli, Elena, additional, Mihailović-Vučinić, Violeta, additional, Rottoli, Paola, additional, Grubanovic, Aleksandar, additional, Müller, Annegret, additional, Jochens, Arne, additional, Tittmann, Lukas, additional, Schnerch, Jasmin, additional, Olivieri, Carmela, additional, Fischer, Annegret, additional, Jovanovic, Dragana, additional, Filipovic, Snežana, additional, Videnovic-Ivanovic, Jelica, additional, Bresser, Paul, additional, Jonkers, René, additional, O'Reilly, Kate, additional, Ho, Ling-Pei, additional, Gaede, Karoline I., additional, Zabel, Peter, additional, Dubaniewicz, Anna, additional, Marshall, Ben, additional, Kieszko, Robert, additional, Milanowski, Janusz, additional, Günther, Andreas, additional, Weihrich, Anette, additional, Petrek, Martin, additional, Kolek, Vitezslav, additional, Keane, Michael P., additional, O'Beirne, Sarah, additional, Donnelly, Seamas, additional, Haraldsdottir, Sigridur Olina, additional, Jorundsdottir, Kristin B., additional, Costabel, Ulrich, additional, Bonella, Francesco, additional, Wallaert, Benoît, additional, Grah, Christian, additional, Peroš-Golubičić, Tatjana, additional, Luisetti, Mauritio, additional, Kadija, Zamir, additional, Pabst, Stefan, additional, Grohé, Christian, additional, Strausz, János, additional, Vašáková, Martina, additional, Sterclova, Martina, additional, Millar, Ann, additional, Homolka, Jiří, additional, Slováková, Alena, additional, Kendrick, Yvonne, additional, Crawshaw, Anjali, additional, Wuyts, Wim, additional, Spencer, Lisa, additional, Pfeifer, Michael, additional, Valeyre, Dominique, additional, Poletti, Venerino, additional, Wirtz, Hubertus, additional, Prasse, Antje, additional, Schreiber, Stefan, additional, Krawczak, Michael, additional, and Müller-Quernheim, Joachim, additional
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- 2018
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45. Apparent IgE negative anaphylactic reaction to banana combined with kiwi allergy - complementary diagnostic value of purified single banana allergens
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Jappe, Uta, Nikolić, Jasna, Opitz, Annika, Homann, Arne, Zabel, Peter, and Gavrović-Jankulović, Marija
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- 2016
46. Physical activity, airway resistance and small airway dysfunction in severe asthma
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Bahmer, Thomas, primary, Waschki, Benjamin, additional, Schatz, Fee, additional, Herzmann, Christian, additional, Zabel, Peter, additional, Kirsten, Anne-Marie, additional, Rabe, Klaus F., additional, and Watz, Henrik, additional
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- 2016
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47. Regional lung response to bronchodilator reversibility testing determined by electrical impedance tomography in chronic obstructive pulmonary disease
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Vogt, Barbara, primary, Zhao, Zhanqi, additional, Zabel, Peter, additional, Weiler, Norbert, additional, and Frerichs, Inéz, additional
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- 2016
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48. Suppressorzellen myeloischen Ursprungs (Myeloid-derived suppressor cells) im Blut von Krebspatienten enthalten unreife neutrophile Granulozyten mit eingeschränkten Migrationseigenschaften
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Trellakis, Sokratis, Lang, Stephan, Elian, Motaz, Bruderek, Kirsten, Schmaltz, Dominik, Suttmann, Henrik, Schenck, Marcus, Welling, Jürgen, Zabel, Peter, and Brandau, Sven
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ddc: 610 ,610 Medical sciences ,Medicine - Abstract
Einleitung: In murinen Tumormodellen wurden immunsuppressive unreife myeloide Zellen monozytären und granulozytären Ursprungs nachgewiesen und als so genannte MDSC (myeloid-derived suppressor cells) definiert. Die Identität und Funktion von MDSC in Krebspatienten ist Gegenstand aktueller Forschung. Einige Autoren schlagen vor, dass reife aktivierte Neutrophile im peripheren Blut humane MDSC repräsentieren. Methoden: Wir analysierten das periphere Blut von 103 Patienten mit unterschiedlichen Krebstypen aus der HNO, Urologie und Pulmologie. Neutrophile Granulozyten wurden über Immunphänotypisierung und funktionelle Untersuchungen charakterisiert. Ergebnisse: Basierend auf den Sedimentationseigenschaften in der Dichtegradient-Zentrifugation wurde eine Untergruppe von polymorphkernigen Granulozyten (PMN) mit geringerer Dichte identifiziert, isoliert und weiter charakterisiert. In diesen PMN geringerer Dichte wurden vermehrt unreife PMN gefunden. Passend zu ihrem unreiferen Zustand zeigten diese PMN im Vergleich zu den konventionellen PMN eine veränderte Expression bestimmter Oberflächenmarker und eine verminderte Apoptose. Außerdem wurden bei ihnen verringerte Effektorfunktionen wie z. B. Oxidative Burst festgestellt. Die Suppression der Produktion von IFN-γ und der Proliferation von polyklonal stimulierten T-Zellen qualifiziert diese PMN geringerer Dichte als humane MDSC. Die von uns identifizierten humanen MDSC zeigten eine deutlich verminderte Chemotaxis Richtung Tumormedium und reduzierte Expression der Chemokinrezeptoren CXCR1 und CXCR2, welche normalerweise zum Austritt der PMN aus dem Blutstrom und zur Gewebeinfiltration erforderlich sind. Schlussfolgerungen: Unsere Studie zeigt eine Akkumulation und Persistenz von langlebigen unreifen granulozytären MDSC mit T-Zell suppressiver Funktion und eingeschränkten Migrationseigenschaften im peripheren Blut von Krebspatienten., GMS Current Posters in Otorhinolaryngology - Head and Neck Surgery; 7:Doc06; ISSN 1865-1038
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- 2011
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49. Placenta-derived conditioned medium with anti-tumor properties on human NSCLC
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Lang, Dagmar S., primary, Marwitz, Sebastian, additional, Zeiser, Tobias, additional, Seehase, Sophie, additional, Watermann, Iris, additional, Vollmer, Ekkehard, additional, Reck, Martin, additional, Zabel, Peter, additional, and Goldmann, Torsten, additional
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- 2015
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50. Staubbelastugen in Mühlen zur Futtermittelherstellung
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Weiler, Stephan W, Ehrbrecht, Julia, Köhler, Ina, Riccò, Matteo, Beck, Christoph, Titjie, Torsten, Karlsdorf, Barbara, Zabel, Peter, Kessel, Richard, and Mark, Anke Van
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- 2007
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