Author: "Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])" - Searchworks@Jio Institute Digital Library Search Results

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654 results on '"Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])"'

1. HbF-promoting polymorphisms may specifically reduce the residual risk of cerebral vasculopathy in SCA children with alpha-thalassemia

Author: Philippe Connes, Céline Renoux, Abdourahim Chamouine, Yves Bertrand, Françoise Bernaudin, Nathalie Bonello-Palot, Catherine Badens, Philippe Joly, Serge Pissard, Gall, Valérie, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Référence des Syndromes Drépanocytaires Majeurs, Thalassémies et Autres pathologies rares du globule rouge et de l’Erythropoïèse, Assistance Publique - Hôpitaux de Marseille (APHM), Hôpital Henri Mondor, Centre Hospitalier de Mayotte, Centre Hospitalier Intercommunal de Créteil (CHIC), Institut d'hématologie et d'oncologie pédiatrique [CHU - HCL] (IHOPe), Hospices Civils de Lyon (HCL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, congenital, hereditary, and neonatal diseases and abnormalities, Physiology, Anemia, Sickle Cell, [SDV.GEN] Life Sciences [q-bio]/Genetics, Alpha-thalassemia, Disease, Quantitative trait locus, Polymorphism, Single Nucleotide, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, alpha-Thalassemia, hemic and lymphatic diseases, Physiology (medical), Genotype, medicine, Humans, Child, Fetal Hemoglobin, [SDV.GEN]Life Sciences [q-bio]/Genetics, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, business.industry, Hematology, medicine.disease, Sickle cell anemia, Residual risk, Red blood cell, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Immunology, Female, Cardiology and Cardiovascular Medicine, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030215 immunology
Abstract: International audience; Sickle cell anemia (SCA) is a disease characterized by abnormal red blood cell rheology. Because of their effects on HbS polymerization and red blood cell deformability, alpha-thalassemia and the residual HbF level are known genetic modifiers of the disease. The aim of our study was to determine if the number of HbF quantitative trait loci (QTL) would also favor a specific sub-phenotype of SCA as it is the case for alpha-thalassemia. Our results confirmed that alpha-thalassemia protected from cerebral vasculopathy but increased the risk for frequent painful vaso-occlusive crises. We also showed that more HbF-QTL may provide an additional and specific protection against cerebral vasculopathy but only for children with alpha-thalassemia (-α/αα or -α/-α genotypes).
Published: 2021

2. Two new reliable immunohistochemical methods for simultaneous identification of capillaries, the three types of fibers and basal lamina in human skeletal muscle

Author: Julien Verney, Bruno Pereira, Mélina Bailly, Amandine Boileau, Bogdan Galusca, David Thivel, Natacha Germain, Daniel Courteix, Christophe Hourdé, Léonard Féasson, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Biostatistiques [CHU Clermont-Ferrand], Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), CHU Clermont-Ferrand-CHU Clermont-Ferrand, Troubles du Comportement Alimentaire, Addictions et Poids Extêmes (TAPE - TCA), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), TAPE laboratory [Saint-Étienne], and Université Jean Monnet [Saint-Étienne] (UJM)
Subjects: 0301 basic medicine, Histology, Capillary action, Muscle Fibers, Skeletal, Antigens, CD34, Basement Membrane, 03 medical and health sciences, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], medicine, Humans, Capillary supply, Fiber, Muscle fibre, Molecular Biology, Combined staining method, Reproducibility, 030102 biochemistry & molecular biology, Chemistry, Antibodies, Monoclonal, Skeletal muscle, Cell Biology, Immunohistochemistry, Muscle fiber type, Capillaries, Medical Laboratory Technology, 030104 developmental biology, medicine.anatomical_structure, CD31, Basal lamina, Metabolic profile, Biomedical engineering
Abstract: International audience; Capillary network of skeletal muscle has a crucial role in oxygen supply and is strongly associated with the phenotype and metabolic profile of muscle fibers. Abundant literature has explored capillarization of skeletal muscle in different populations and in response to different interventions. Capillary and fiber type identification techniques have considerably evolved over the last decades, but to the best of our knowledge, no validated immunohistochemical method has yet been developed to simultaneously identify capillaries (using CD31), the three different muscle fiber types, and basal lamina. Nine human muscle biopsies of vastus lateralis were stained using 5 different methods to test: the reliability of different CD31 antibodies for capillary identification, the reliability between single-section or serial-section methods, and the intra-experimenter reproducibility in visual detection of capillaries. High reliability for the different antibodies directed against capillaries was observed for capillary contacts (CC) measurements (intra-class correlations (ICC) [ICC95%] of 0.89 [0.72; 0.96] for type I fibers, 0.93 [0.81; 0.97] for type IIA fibers, 0.88 [0.71; 0.96] for type IIX fibers, 0.95 [0.86; 0.98] for all fiber types) as well as a high level of similarity between single and serial sections methods. A strong similarity in capillary analysis between the different methods was obtained for each sample measurements. Analysis of Lin's concordance correlation coefficients and Bland and Altman's graphics showed a strong intra-experimenter reproducibility. This article proposes two time- and tissue-sparing immunohistochemical methods to accurately assess a complete fiber typing (type I, IIA, and IIX) along with muscle capillarization on a single muscle section.
Published: 2020

3. Skeletal Ryanodine Receptors Are Involved in Impaired Myogenic Differentiation in Duchenne Muscular Dystrophy Patients

Author: Jean-Marie Cuisset, Jérôme Cottalorda, Stefan Matecki, Gérald Hugon, Alain Lacampagne, Pierre Meyer, Cécile Notarnicola, Hugues Chevassus, Albano C. Meli, Pascal Sabouraud, François Rivier, Léonard Féasson, Mirna Khalil, Gilles Carnac, Jérémy Salvador, Claude Cances, Isabelle Desguerre, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre d'investigation clinique de Toulouse (CIC 1436), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris Cité (UPC), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre Hospitalier Universitaire de Reims (CHU Reims), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Pôle Santé publique et médecine publique [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Université Paris Cité (UPCité), Herrada, Anthony, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Université de Paris (UP)
Subjects: Male, Duchenne muscular dystrophy, Muscle Fibers, Skeletal, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Muscle Development, Calcium in biology, Dystrophin, Myoblasts, 0302 clinical medicine, Myocyte, Biology (General), Child, Spectroscopy, Cells, Cultured, 0303 health sciences, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Ryanodine receptor, Myogenesis, Cell Differentiation, General Medicine, musculoskeletal system, Computer Science Applications, Chemistry, Sarcoplasmic Reticulum, medicine.anatomical_structure, Child, Preschool, myogenesis, tissues, Intracellular, musculoskeletal diseases, medicine.medical_specialty, congenital, hereditary, and neonatal diseases and abnormalities, QH301-705.5, Catalysis, Article, Inorganic Chemistry, Tacrolimus Binding Proteins, 03 medical and health sciences, ryanodine receptor, endomysial fibrosis, human, Internal medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine, Humans, Calcium Signaling, Physical and Theoretical Chemistry, Muscle, Skeletal, Molecular Biology, QD1-999, 030304 developmental biology, RYR1, business.industry, Organic Chemistry, Skeletal muscle, Ryanodine Receptor Calcium Release Channel, medicine.disease, Muscular Dystrophy, Duchenne, Endocrinology, Calcium, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, 030217 neurology & neurosurgery
Abstract: Duchenne muscular dystrophy (DMD) is characterized by progressive muscle wasting following repeated muscle damage and inadequate regeneration. Impaired myogenesis and differentiation play a major role in DMD as well as intracellular calcium (Ca2+) mishandling. Ca2+ release from the sarcoplasmic reticulum is mostly mediated by the type 1 ryanodine receptor (RYR1) that is required for skeletal muscle differentiation in animals. The study objective was to determine whether altered RYR1-mediated Ca2+ release contributes to myogenic differentiation impairment in DMD patients. The comparison of primary cultured myoblasts from six boys with DMD and five healthy controls highlighted delayed myoblast differentiation in DMD. Silencing RYR1 expression using specific si-RNA in a healthy control induced a similar delayed differentiation. In DMD myotubes, resting intracellular Ca2+ concentration was increased, but RYR1-mediated Ca2+ release was not changed compared with control myotubes. Incubation with the RYR-calstabin interaction stabilizer S107 decreased resting Ca2+ concentration in DMD myotubes to control values and improved calstabin1 binding to the RYR1 complex. S107 also improved myogenic differentiation in DMD. Furthermore, intracellular Ca2+ concentration was correlated with endomysial fibrosis, which is the only myopathologic parameter associated with poor motor outcome in patients with DMD. This suggested a potential relationship between RYR1 dysfunction and motor impairment. Our study highlights RYR1-mediated Ca2+ leakage in human DMD myotubes and its key role in myogenic differentiation impairment. RYR1 stabilization may be an interesting adjunctive therapeutic strategy in DMD.
Published: 2021

4. Effects of prismatic adaptation on balance and postural disorders in patients with chronic right stroke: protocol for a multicentre double-blind randomised sham-controlled trial

Author: Hugues, Aurélien, Guinet-Lacoste, Amandine, Bin, Sylvie, Villeneuve, Laurent, Lunven, Marine, Pérennou, Dominic, Giraux, Pascal, Foncelle, Alexandre, Rossetti, Yves, Jacquin-Courtois, Sophie, Luauté, Jacques, Rode, Gilles, Hospices Civils de Lyon (HCL), Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Hôpital Henry Gabrielle [CHU - HCL], Université de Lyon, Hôpital Albert Chenevier, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire de Psychologie et NeuroCognition (LPNC ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Centre Hospitalier Universitaire [Grenoble] (CHU), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Malbec, Odile
Subjects: [SDV]Life Sciences [q-bio], Stroke Rehabilitation, rehabilitation medicine, adult neurology, Adaptation, Physiological, stroke, [SDV] Life Sciences [q-bio], Double-Blind Method, Humans, Multicenter Studies as Topic, Medicine, Postural Balance, Randomized Controlled Trials as Topic
Abstract: International audience; Introduction: Patients with right stroke lesion have postural and balance disorders, including weight-bearing asymmetry, more pronounced than patients with left stroke lesion. Spatial cognition disorders post-stroke, such as misperceptions of subjective straight-ahead and subjective longitudinal body axis, are suspected to be involved in these postural and balance disorders. Prismatic adaptation has showed beneficial effects to reduce visuomotor disorders but also an expansion of effects on cognitive functions, including spatial cognition. Preliminary studies with a low level of evidence have suggested positive effects of prismatic adaptation on weight-bearing asymmetry and balance after stroke. The objective is to investigate the effects of this intervention on balance but also on postural disorders, subjective straight-ahead, longitudinal body axis and autonomy in patients with chronic right stroke lesion.Methods and analysis: In this multicentre randomised double-blind sham-controlled trial, we will include 28 patients aged from 18 to 80 years, with a first right supratentorial stroke lesion at chronic stage (≥12 months) and having a bearing ≥60% of body weight on the right lower limb. Participants will be randomly assigned to the experimental group (performing pointing tasks while wearing glasses shifting optical axis of 10 degrees towards the right side) or to the control group (performing the same procedure while wearing neutral glasses without optical deviation). All participants will receive a 20 min daily session for 2 weeks in addition to conventional rehabilitation. The primary outcome will be the balance measured using the Berg Balance Scale. Secondary outcomes will include weight-bearing asymmetry and parameters of body sway during static posturographic assessments, as well as lateropulsion (measured using the Scale for Contraversive Pushing), subjective straight-ahead, longitudinal body axis and autonomy (measured using the Barthel Index).Ethics and dissemination: The study has been approved by the ethical review board in France. Findings will be submitted to peer-reviewed journals relative to rehabilitation or stroke.Trial registration number: NCT03154138.
Published: 2021

5. Beneficial Effects of High Intensity Interval Training and/or Linseed Oil Supplementation to Limit Obesity-Induced Oxidative Stress in High Fat Diet-Fed Rats

Author: Laurie Josset, Claire Plissonneau, Carole Groussard, Vincent Pialoux, Nicolas Barnich, Mathilde Mura, Guillaume Mairesse, Nathalie Boisseau, Frédéric Capel, Guillaume Chesneau, Etienne Gouraud, Laboratoire Mouvement Sport Santé (M2S), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Valorex, This research was funded by grants from Valorex. The authors are also supported by the Ministère de la Recherche et de la Technologie (M2iSH and AME2P Unit), Inserm (UMR 1071), INRAE (USC-2018) and by the French government IDEX-ISITE initiative 16-IDEX-0001 (CAP 20-25), I-SITE project (CAP 2025) of the University of Clermont Auvergne., ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Jonchère, Laurent, and CAP 20-25 - - CAP 20-252016 - ANR-16-IDEX-0001 - IDEX - VALID
Subjects: Male, obesity, Antioxidant, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Adipose tissue, High-Intensity Interval Training, medicine.disease_cause, Interval training, Antioxidants, 0302 clinical medicine, Subcutaneous Tissue, Linseed oil, oxidative stress, TX341-641, 2. Zero hunger, 0303 health sciences, Nutrition and Dietetics, biology, Chemistry, high fat diet, food and beverages, Catalase, Up-Regulation, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, Liver, High-intensity interval training, Subcutaneous tissue, medicine.medical_specialty, food.ingredient, Linseed Oil, high intensity interval training, Article, 03 medical and health sciences, food, Internal medicine, medicine, Animals, Rats, Wistar, 030304 developmental biology, Nitrates, Nutrition. Foods and food supply, Superoxide Dismutase, Feeding Behavior, linseed oil supplementation, Endocrinology, Dietary Supplements, biology.protein, 030217 neurology & neurosurgery, Oxidative stress, Biomarkers, Food Science
Abstract: International audience; High-intensity interval training (HIIT) and linseed oil (LO) supplementation are effective strategies to reduce obesity-induced oxidative stress. Our aim was to determine whether the HIIT + LO combination prevents obesity-induced oxidative stress in high fat diet (HFD)-fed rats. HFD-fed 8-week-old, male, Wistar rats were subdivided in four groups: HFD, LO (2% of sunflower oil replaced with 2% of LO in the HFD), HIIT (4 days/week for 12 weeks), and HIIT + LO. Wistar rats fed a low-fat diet (LFD) were used as controls. Epididymal and subcutaneous adipose tissue, gastrocnemius muscle, liver, and plasma samples were collected to measure oxidative stress markers (AOPP, oxLDL), antioxidant (SOD, CAT, and GPx activities) and pro-oxidant (NOx and XO) enzyme activities. Compared with the LFD, the HFD altered the pro/antioxidant status in different tissues (increase of AOPP, oxLDL, SOD and catalase activities in plasma, and SOD activity increase in liver and decrease in adipose tissues) but not in gastrocnemius. LO upregulated CAT activity and decreased NOx in liver. HIIT alleviated HFD negative effects in liver by reducing SOD and NOx activities. Moreover, the HIIT + LO combination potentiated SOD activity upregulation in subcutaneous tissue. HIIT and LO supplementation have independent beneficial effects on the pro/antioxidant balance. Their association promotes SOD activity in subcutaneous adipose tissue.
Published: 2021

6. Effect of race distance on performance fatigability in male trail and ultra‐trail runners

Author: Benjamin Singh, Thomas Lapole, Thibault Besson, Nicolas Royer, John Temesi, Vincent Martin, Diana Rimaud, Callum G. Brownstein, Léonard Féasson, Audrey Parent, Loïc Espeit, Guillaume Y. Millet, Faculty of Health & Life Sciences, Northumbria University, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Department of Biological Sciences, Université du Québec à Montréal, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Centre de Référence des Maladies Neuromusculaires Rares, Unité de Myologie, Centre Hospitalier Universitaire (CHU), University of Northumbria at Newcastle [United Kingdom], Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Temesi, John, Besson, Thibault, Parent, Audrey, Singh, Benjamin, Martin, Vincent, Brownstein, Callum G, Espeit, Loïc, Royer, Nicolas, Rimaud, Diana, Lapole, Thomas, Féasson, Léonard, and Millet, Guillaume Y
Subjects: Adult, Male, medicine.medical_specialty, Nerve stimulation, Time Factors, medicine.medical_treatment, Physical Therapy, Sports Therapy and Rehabilitation, Athletic Performance, Plantar flexion, Running, Leukocyte Count, 03 medical and health sciences, 0302 clinical medicine, Voluntary contraction, Internal medicine, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Humans, Orthopedics and Sports Medicine, Muscle, Skeletal, Creatine Kinase, Exercise duration, knee extensors, ComputingMilieux_MISCELLANEOUS, Knee extensors, Electromyography, business.industry, 030229 sport sciences, Evoked Potentials, Motor, Transcranial Magnetic Stimulation, C600, Electric Stimulation, Transcranial magnetic stimulation, C-Reactive Protein, Torque, Muscle Fatigue, plantar flexors, Physical Endurance, Cardiology, Silent period, fatigue, Tibial Nerve, business, Femoral Nerve, 030217 neurology & neurosurgery, Muscle Contraction
Abstract: The etiology of changes in lower-limb neuromuscular function, especially to the central nervous system, may be affected by exercise duration. Direct evidence is lacking as few studies have directly compared different race distances. This study aimed to investigate the etiology of deficits in neuromuscular function following short versus long trail-running races. Thirty-two male trail runners completed one of five trail-running races as LONG (>100 km) or SHORT (
Published: 2021

7. Formation tracking of target moving on natural surfaces

Author: H. Nadour, Pierre Legreneur, Lionel Reveret, Nicolas Marchand, GIPSA - COntrol, PErception, Robots, navigation and Intelligent Computing (GIPSA-COPERNIC), GIPSA Pôle Sciences des Données (GIPSA-PSD), Grenoble Images Parole Signal Automatique (GIPSA-lab), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Grenoble Images Parole Signal Automatique (GIPSA-lab), Université Grenoble Alpes (UGA), Calcul des Variations, Géométrie, Image (CVGI), Laboratoire Jean Kuntzmann (LJK), Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), AirCap Regional project funded by French ARA Region, Capture and Analysis of Shapes in Motion (MORPHEO), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Jean Kuntzmann (LJK), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Computer science, Real-time computing, 3D reconstruction, Swarm behaviour, UAVs control, Solid modeling, formation control, Collision, Tracking (particle physics), [SPI.AUTO]Engineering Sciences [physics]/Automatic, Sight, monitoring, Position (vector), [INFO.INFO-AU]Computer Science [cs]/Automatic Control Engineering, surveillance, target track-ing, Focus (optics), target tracking, film-making
Abstract: International audience; In this paper, we focus mainly on formation control of a swarm of UAV's tracking a moving target on a non regular mountainous surface. The main objective is to show how to dispatch the whole swarm of the quadrotors around the target (climber) with respect to the natural environment (mountain) taking advantage as much as possible from the free space surrounding the climber. The formation shape adapts with the neighbour environment topology, so that it avoids the collision with the environment and the concealing of the target from the UAV's cameras sights, and ensures as well the formation compactness and its closure to the target respecting the agent-agent and the agent-target security distances. The algorithm to define the free and safe hovering space (SHS) is then demonstrated with other two-steps algorithm to determine for each agent its desired position within the SHS. This work, that is considered as an introduction for upcoming works, does not miss to provide a simple control for each individual agent based on an established and corrected approximate dynamic model. The main idea to resolve this problem is explained in 2D and will be expanded for 3D environment, with other necessary and complementary enhancements. Keywords: UAVs control, formation control, target tracking, surveillance, film-making, 3D reconstruction, monitoring, quadrotor modeling.
Published: 2021

8. Sex Differences in Neuromuscular Fatigue and Changes in Cost of Running after Mountain Trail Races of Various Distances

Author: Audrey Parent, Guillaume Y. Millet, Vincent Martin, Callum G. Brownstein, Thomas Lapole, Benjamin Singh, Giorgio Varesco, Thibault Besson, Loïc Espeit, John Temesi, Diana Rimaud, Frederic Sabater Pastor, Nicolas Royer, Jérémy Rossi, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Department of Biological Sciences, Université du Québec à Montréal, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Faculty of Health & Life Sciences, Northumbria University, Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), University of Northumbria at Newcastle [United Kingdom], Besson, Thibault, Parent, Audrey, Brownstein, Callum G, Espeit, Loic, Lapole, Thomas, Martin, Vincent, Royer, Nicolas, Rimaud, Diana, Sabater Pastor, Frederic, Singh, Benjamin, Varesco, Giorgio, Rossi, Jeremy, Temesi, John, and Millet, Guillaume Y
Subjects: Male, medicine.medical_specialty, Competitive Behavior, males, Physical Therapy, Sports Therapy and Rehabilitation, Marathon Running, Plantar flexion, trail running, Oxygen Consumption, Internal medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], medicine, Humans, Orthopedics and Sports Medicine, Knee, Treadmill, Respiratory exchange ratio, Creatine Kinase, ComputingMilieux_MISCELLANEOUS, Sex Characteristics, Knee extensors, business.industry, Electromyography, Foot, Pulmonary Gas Exchange, C100, females, Evoked Potentials, Motor, C600, Transcranial Magnetic Stimulation, Electric Stimulation, energy cost, Peripheral, C-Reactive Protein, Neuromuscular fatigue, Torque, Muscle Fatigue, Breathing, Cardiology, Running economy, Physical Endurance, fatigability, Female, business, Energy Metabolism
Abstract: Introduction: Women have been shown to experience less neuromuscular fatigue than men in knee extensors (KE) and less peripheral fatigue in plantar flexors (PF) after ultratrail running, but it is unknown if these differences exist for shorter trail running races and whether this may impact running economy. The purpose of this study was to characterize sex differences in fatigability over a range of running distances and to examine possible differences in the postrace alteration of the cost of running (Cr). Methods: Eighteen pairs of men and women were matched by performance after completing different races ranging from 40 to 171 km, divided into SHORT versus LONG races (100 km, respectively). Neuromuscular function and Cr were tested before and after each race. Neuromuscular function was evaluated on both KE and PF with voluntary and evoked contractions using electrical nerve (KE and PF) and transcranial magnetic (KE) stimulation. Oxygen uptake, respiratory exchange ratio, and ventilation were measured on a treadmill and used to calculate Cr. Results: Compared with men, women displayed a smaller decrease in maximal strength in KE (−36% vs −27%, respectively, P < 0.01), independent of race distance. In SHORT only, women displayed less peripheral fatigue in PF compared with men (Δ peak twitch: −10% vs −24%, respectively, P < 0.05). Cr increased similarly in men and women. Conclusions: Women experience less neuromuscular fatigue than men after both “classic” and “extreme” prolonged running exercises but this does not impact the degradation of the energy Cr. Refereed/Peer-reviewed
Published: 2021

9. The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

Author: DUPUIT, Marine, Chavanelle, Vivien, Chassaing, Benoit, Perriere, Fanny, Etienne, Monique, Plissonneau, Claire, Boscaro, Audrey, Barnich, Nicolas, Pialoux, Vincent, Maugard, Thierry, Le Joubioux, Florian, Peltier, Sébastien, Sirvent, Pascal, Otero, Yolanda, BOISSEAU, Nathalie, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Valbiotis R & D [Riom], Valbiotis SAS, Partenaires INRAE-Partenaires INRAE, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Valbiotis R & D [La Rochelle], Valbiotis, Kenneth Rainin Foundation, ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018), European Project: ERC-2018-StG804135, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), and Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, plant extract supplementation, Diet, High-Fat, Weight Gain, Article, MESH: obesity / therapy, Prediabetic State, MESH: high-intensity interval training, Physical Conditioning, Animal, microbiota, Animals, TX341-641, Obesity, Intestinal Mucosa, Rats, Wistar, high-intensity interval training, MESH: polyphenols / administration & dosage, body composition, Plant Extracts, MESH: physical conditioning, animal / methods, Nutrition. Foods and food supply, digestive, oral, and skin physiology, Polyphenols, food and beverages, MESH: dietary supplements, Combined Modality Therapy, Gastrointestinal Microbiome, Rats, Disease Models, Animal, Diabetes Mellitus, Type 2, MESH: plant extracts / administration & dosage, Dietary Supplements, glycemic control, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, MESH: rats, Wistar
Abstract: Obesity and prediabetes are the two strongest risk factors of type 2 diabetes. It has been reported that TOTUM-63, a polyphenol-rich plant extract, has beneficial effects on body weight (BW) and insulin resistance in mice fed a high fat diet (HFD). The study aim was to determine whether high-intensity interval training (HIIT) and/or TOTUM-63 supplementation improved body composition and glycemic control and gut microbiota composition in a Western diet-induced obesity rat model. Wistar rats received a standard diet (CTRL; control; n = 12) or HFD (HFD; n = 48) for 16 weeks. Then, HFD rats were divided in four groups: HFD, HFD + TOTUM-63 (T63), HFD + HIIT (HIIT), and HFD + HIIT +T63 (HIIT + T63). Training was performed 4 days/week for 12 weeks. TOTUM-63 was included in diet composition (2%). The HIIT + T63 combination significantly limited BW gain, without any energy intake modulation, and improved glycemic control. BW variation was correlated with increased α-diversity of the colon mucosa microbiota in the HIIT + T63 group. Moreover, the relative abundance of Anaeroplasma, Christensenellaceae and Oscillospira was higher in the HIIT + T63 group. Altogether, these results suggest that the HIIT and TOTUM-63 combination could be proposed for the management of obesity and prediabetes.
Published: 2021

10. Muscle Proteomic and Transcriptomic Profiling of Healthy Aging and Metabolic Syndrome in Men

Author: Gueugneau, Marine, Coudy-Gandilhon, Cécile, Chambon, Christophe, Verney, Julien, Taillandier, Daniel, Combaret, Lydie, Polge, Cécile, Walrand, Stéphane, Roche, Frédéric, Barthélémy, Jean-Claude, Féasson, Léonard, Béchet, Daniel, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Hôpital Nord - Faculté de MédecineJacques Lisfranc, Service de Physiologie Clinique et de l'Exercice, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de physiologie clinique, unité de myologie, CHU Saint-Etienne, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), European Commission MyoAge EC Fp7 CT-223756Caisse d'Epargne Rhone Alpes (CERA Sarcopenie) 30European CommissionRegion Auvergne-Rhone-AlpesEuropean Commission, ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), Service de Physiologie Clinique et de l'Exercice [CHU de Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Santé Ingénierie Biologie Saint-Etienne (SAINBIOSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Proteomics, QH301-705.5, proteome, aging, Article, metabolic syndrome, sarcopenia, Chemistry, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Biology (General), skeletal muscle, Muscle, Skeletal, Glycolysis, QD1-999, transcriptome
Abstract: International audience; (1) Background: Aging is associated with a progressive decline in muscle mass and function. Aging is also a primary risk factor for metabolic syndrome, which further alters muscle metabolism. However, the molecular mechanisms involved remain to be clarified. Herein we performed omic profiling to decipher in muscle which dominating processes are associated with healthy aging and metabolic syndrome in old men. (2) Methods: This study included 15 healthy young, 15 healthy old, and 9 old men with metabolic syndrome. Old men were selected from a well-characterized cohort, and each vastus lateralis biopsy was used to combine global transcriptomic and proteomic analyses. (3) Results: Over-representation analysis of differentially expressed genes (ORA) and functional class scoring of pathways (FCS) indicated that healthy aging was mainly associated with upregulations of apoptosis and immune function and downregulations of glycolysis and protein catabolism. ORA and FCS indicated that with metabolic syndrome the dominating biological processes were upregulation of proteolysis and downregulation of oxidative phosphorylation. Proteomic profiling matched 586 muscle proteins between individuals. The proteome of healthy aging revealed modifications consistent with a fast-to-slow transition and downregulation of glycolysis. These transitions were reduced with metabolic syndrome, which was more associated with alterations in NADH/NAD+ shuttle and β-oxidation. Proteomic profiling further showed that all old muscles overexpressed protein chaperones to preserve proteostasis and myofiber integrity. There was also evidence of aging-related increases in reactive oxygen species but better detoxifications of cytotoxic aldehydes and membrane protection in healthy than in metabolic syndrome muscles. (4) Conclusions: Most candidate proteins and mRNAs identified herein constitute putative muscle biomarkers of healthy aging and metabolic syndrome in old men.
Published: 2021

11. Actualisation des recommandations françaises de traitement du pemphigus

Author: Catherine Prost-Squarcioni, Olivier Dereure, Vivien Hébert, C. Le Roux-Villet, Fabienne Jouen, E. Le Bidre, M-P Konstantinou, Frédéric Caux, Nadège Cordel, L Jelti, Gaëlle Quéreux, Catherine Picard-Dahan, J. Plée, Christophe Bedane, Michel D'Incan, Denis Jullien, M-A Viguier, Sophie Duvert-Lehembre, Bruno Sassolas, Marina Alexandre, I Bourgault Villada, E. Tancrede-Bohin, Pascal Joly, J-D Bouaziz, Saskia Ingen-Housz-Oro, M-S Doutre, Sébastien Debarbieux, M-A Richard, Emmanuel Delaporte, B. Mellottee, P. Bernard, Service de dermatologie [Avicenne], Université Paris 13 (UP13)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay, Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Service de dermatologie [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Service de dermatologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Service de Dermatologie [Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU), Fonctionnement et évolution des systèmes écologiques (FESE), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques er émergentes (TransVIHMI), Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Institut Laue-Langevin (ILL), Imagerie Moléculaire et Stratégies Théranostiques (IMoST), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), CHU Clermont-Ferrand, Laboratoire de Chimie Industrielle, École Nationale d'Ingénieurs de Sfax | National School of Engineers of Sfax (ENIS), CHU Bordeaux [Bordeaux], CHU Pointe-à-Pitre/Abymes [Guadeloupe], Hopital de Bohars - CHRU Brest (CHU - BREST ), Hôpital Robert Debré, CRCM, Université Paris7, Assistance Publique - Hôpitaux de Paris, Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), ArcelorMittal Maizières Research SA, ArcelorMittal, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen], École normale supérieure - Paris (ENS Paris), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de Recherche pour le Développement (IRD)-Université de Yaoundé I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), ILL, Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Hôpital Charles Nicolle [Rouen], Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes (TransVIHMI), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: medicine.medical_specialty, business.industry, MEDLINE, [SDV.CAN]Life Sciences [q-bio]/Cancer, Dermatology, medicine.disease, 030207 dermatology & venereal diseases, 03 medical and health sciences, Pemphigus, 0302 clinical medicine, 030220 oncology & carcinogenesis, Severity of illness, medicine, Intensive care medicine, business, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

12. Pro-oxidant/Antioxidant Balance During a Prolonged Exposure to Moderate Altitude in Athletes Exhibiting Exercise-Induced Hypoxemia at Sea-Level

Author: Antoine Raberin, Vincent Pialoux, Elie Nader, Gauthier Alfonsi, Fabienne Durand, Chantal L. Rytz, Jorge Lopez Ayerbe, Patrick Mucci, Laboratoire Européen Performance Santé Altitude (LEPSA), Université de Perpignan Via Domitia (UPVD), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, University of Calgary, Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Université de Lille, Univ. Artois, Univ. Littoral Côte d’Opale, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369, Laboratoire Européen Performance Santé Altitude [LEPSA], Laboratoire Interuniversitaire de Biologie de la Motricité [LIBM ], Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 [URePSSS], Institut Universitaire de France [IUF], Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Université d'Artois (UA)-Université de Lille-Université du Littoral Côte d'Opale (ULCO)
Subjects: medicine.medical_specialty, [SDV]Life Sciences [q-bio], medicine.disease_cause, General Biochemistry, Genetics and Molecular Biology, Article, Nitric oxide, Hypoxemia, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, medicine, arterial desaturation, oxidative stress, aerobic performance, lcsh:Science, Ecology, Evolution, Behavior and Systematics, chemistry.chemical_classification, reactive oxygen species, biology, business.industry, hypoxia, Glutathione peroxidase, Paleontology, 030229 sport sciences, Hypoxia (medical), Pro-oxidant, acclimatization, Endocrinology, chemistry, Space and Planetary Science, biology.protein, lcsh:Q, medicine.symptom, business, 030217 neurology & neurosurgery, Respiratory minute volume, Oxidative stress
Abstract: This study examined to what extent athletes exhibiting exercise-induced hypoxemia (EIH) possess an altered redox status at rest, in response to exercise at sea level (SL) and during moderate altitude exposure. EIH was defined as a fall in arterial O2 saturation of at least 4% during exercise. Nine endurance athletes with EIH and ten without (NEIH) performed a maximal incremental test under three conditions: SL, one (H1) and five (H2) days after arrival to 2400 m. Gas exchange and peripheral capillary oxygen saturation (SpO2) were continuously monitored. Blood was sampled before exercise and after exercise cessation. Advanced oxidation protein products (AOPP), catalase, ferric-reducing antioxidant power, glutathione peroxidase, superoxide dismutase (SOD) and nitric oxide metabolites (NOx) were measured in plasma by spectrophotometry. EIH athletes had higher AOPP and NOx concentrations at pre- and post-exercise stages compared to NEIH at SL, H2 but not at H1. Only the EIH group experienced increased SOD activity between pre- and post-exercise exercise at SL and H2 but not at H1. EIH athletes had exacerbated oxidative stress compared to the NEIH athletes at SL and H2. These differences were blunted at H1. Oxidative stress did not alter the EIH groups’ aerobic performance and could lead to higher minute ventilation at H2. These results suggest that higher oxidative stress response EIH athletes could be involved in improved aerobic muscle functionality and a greater ventilatory acclimatization during prolonged hypoxia.
Published: 2021

13. Perceptions of the environment moderate the effects of objectively-measured built environment attributes on active transport. An ACTI-Cités study

Author: Julie-Anne Nazare, Camille Perchoux, Franck Hess, Paul Salze, Chantal Simon, Céline Roda, Mehdi Menai, Thierry Feuillet, Jean-Michel Oppert, Serge Hercberg, Christiane Weber, Marine M. Desgeorges, Hélène Charreire, Christophe Enaux, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Image, Ville, Environnement (LIVE), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Nutrition Humaine - Ile de France (CRNH - IDF), Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Sorbonne Paris Nord-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), LAB'URBA (LAB'URBA), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Université Gustave Eiffel, Territoires, Environnement, Télédétection et Information Spatiale (UMR TETIS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de Santé Publique [Avicenne], Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Laboratoire Dynamiques Sociales et Recomposition des Espaces (LADYSS), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Luxembourg Institute of Socio-Economic Research (LISER), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris 1 Panthéon-Sorbonne (UP1)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Université de Paris (UP), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris 8 Vincennes-Saint-Denis (UP8)-Université Paris Nanterre (UPN)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)
Subjects: CITES, genetic structures, Health Policy, media_common.quotation_subject, Transport time, Public Health, Environmental and Occupational Health, 030209 endocrinology & metabolism, Transportation, Sample (statistics), [SHS.GEO]Humanities and Social Sciences/Geography, Moderation, Pollution, 03 medical and health sciences, 0302 clinical medicine, Walkability, Perception, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, 030212 general & internal medicine, Safety, Risk, Reliability and Quality, Construct (philosophy), Psychology, Safety Research, Social psychology, Built environment, media_common
Abstract: International audience; Purpose: Both perceived and objectively-measured attributes of the built environment have been related to active transport. They do not refer, however, to the same construct: objective measurements provide an inventory of a neighborhood?s active-friendly attributes, while the perceptions of individuals refer to their appraisal of their neighborhood opportunities for active travel. Both aspects are complementary in explaining active transport. This study examines the independent associations of perceived and objective environmental attributes with active transport, and whether perceptions moderate the objectively-measured environment active transport relationship.Methods: Based on a sample of 2670 participants from the NutriNet-Sante? Study (France), we examined (i) the concordance between perceptions and objective measurements of residentialbased environmental attributes using kappa statistics, (ii) the associations between perceived and objectively-measured attributes and active transport by trip purpose (commuting and errand) using zero-inflated negative-binomial regressions, and (iii) the potential moderation effect of perceptions on the associations between objectively-measured environmental attributes and active transport. Information related to demographics, perceptions and transport modes was collected in 2013.Results: A poor to fair level of agreement was observed between perceived , objectively -measured environmental attributes; both were associated with active transport for commuting and errands. Environmental correlates differed between the likelihood to engage in active transport and the time spent in transport. Perceptions positively moderated the associations be-tween active transport and objectively-measured walkability and access to facilities. The differ-ences in predicted active transport time between a high versus low perception of the environmental attributes for a given neighborhood ranged from 20 to 55 min for active commuting , from 84 to 37 min for active transport for errands.Conclusion: Associations between objectively-measured environmental attributes and active transport are dependent on individuals & rsquo; perceptions of opportunities provided by their residential neighborhood. Increasing individuals & rsquo; awareness of active-friendly environmental attributes may be crucial for enhancing active transport.
Published: 2021

14. Effects of an Exercise and Nutritional Intervention on Circulating Biomarkers and Metabolomic Profiling During Adjuvant Treatment for Localized Breast Cancer: Results From the PASAPAS Feasibility Randomized Controlled Trial

Author: Vincent Pialoux, Olivier Tredan, Béatrice Fervers, Lidia Delrieu, Adrien Rossary, Florence Caldefie-Chezet, Bénédicte Elena-Herrmann, Agnès Martin, Elodie Jobard, Olivia Febvey-Combes, Marina Touillaud, Magali Morelle, Houda Boumaza, Sophie E. Berthouze, Aude-Marie Foucaut, Marie-Paule Vasson, Patrick Bachmann, ISA NMR Methods for Metabolism - Methodes RMN en métabolomique (2014-2018), Institut des Sciences Analytiques (ISA), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Laboratoire Educations et Pratiques de Santé (LEPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Sorbonne Paris Cité (USPC)-Université Paris 13 (UP13), Centre Léon Bérard [Lyon], Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Health System Analysis Laboratory (GATE), Département cancer environnement (Centre Léon Bérard - Lyon), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université Clermont Auvergne (UCA), Radiations : Défense, Santé, Environnement [Lyon] (Inserm UA8), Institut National de la Santé et de la Recherche Médicale (INSERM), Elena-Herrmann, Bénédicte, Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Unité Ad Hoc INSERM 000008 'Radiations : Défense, Santé, Environnement' [Lyon] (Inserm U1296), and Centre Léon Bérard [Lyon]-Service de Santé des Armées-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Oncology, medicine.medical_specialty, [SDV]Life Sciences [q-bio], Physical exercise, [SDV.CAN]Life Sciences [q-bio]/Cancer, Breast Neoplasms, chemotherapy, lcsh:RC254-282, law.invention, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, breast cancer, [SDV.CAN] Life Sciences [q-bio]/Cancer, Randomized controlled trial, law, Internal medicine, Biomarkers, Tumor, Medicine, Aerobic exercise, Humans, 030212 general & internal medicine, ComputingMilieux_MISCELLANEOUS, Adiponectin, exercise, business.industry, Leptin, biomarkers, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, metabolomics, Physical activity level, 3. Good health, Exercise Therapy, Complementary and alternative medicine, 030220 oncology & carcinogenesis, Exercise and Cancer Treatment, Ceiling effect, Feasibility Studies, Female, business, Research Article
Abstract: Purpose: Exercise has been shown to improve physical and psychological conditions during cancer therapy, but mechanisms remain poorly understood. The purpose of the present study was to report the results of cancer-related biomarkers and metabolomics outcomes from the PASAPAS feasibility study. Methods: In the PASAPAS randomized controlled trial, 61 women beginning adjuvant chemotherapy for localized breast cancer were randomized in a 6-month program of weekly aerobic exercises associated with nutritional counseling versus usual care with nutritional counseling. In the present analysis of 58 women for whom blood samples were available, first, circulating levels of biomarkers (ie, insulin, insulin-like growth factor 1, estradiol, adiponectin, leptin, interleukin-6, and tumor necrosis factor α) were measured at baseline and 6-month follow-up. Changes in biomarkers were compared between exercisers (n = 40) and controls (n = 18) using mixed-effect models. Second, serum metabolites were studied using an untargeted 1H nuclear magnetic resonance spectroscopy, and orthogonal partial least squares analyses were performed to discriminate exercisers and controls at baseline and at 6 months. Results: Over the 6-month intervention, no statistically significant differences were observed between exercisers and controls regarding changes in biomarkers and metabolomic profiles. Conclusion: The present analysis of the PASAPAS feasibility trial did not reveal any improvement in circulating biomarkers nor identified metabolic signatures in exercisers versus controls during adjuvant breast cancer treatment. Larger studies preferably in women with poor physical activity level to avoid ceiling effect, testing different doses and types of exercise on additional biological pathways, could allow to clarify the mechanisms mediating beneficial effects of physical exercise during cancer treatment. Trial registration: ClinicalTrials.gov Identifier: NCT01331772. Registered 8 April 2011, https://clinicaltrials.gov/ct2/show/NCT01331772?term=pasapas&rank=1
Published: 2021

15. Goal-Oriented Monitoring of Cyclosporine Is Effective for Graft-versus-Host Disease Prevention after Hematopoietic Stem Cell Transplantation in Sickle Cell Disease and Thalassemia Major

Author: Kamila Kebaili, Nathalie Garnier, Marie-Pierre Goutagny, Yves Bertrand, Philippe Joly, Cecile Renard, Isabelle Mollet, Alexandra Gauthier, Nathalie Bleyzac, Sylvain Goutelle, Institut d'hématologie et d'oncologie pédiatrique [CHU - HCL] (IHOPe), Hospices Civils de Lyon (HCL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Evaluation et modélisation des effets thérapeutiques, Département biostatistiques et modélisation pour la santé et l'environnement [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: medicine.medical_specialty, Transplantation Conditioning, medicine.medical_treatment, Thalassemia, [SDV]Life Sciences [q-bio], Cell, Graft vs Host Disease, Disease, Hematopoietic stem cell transplantation, Anemia, Sickle Cell, Gastroenterology, 03 medical and health sciences, 0302 clinical medicine, immune system diseases, Internal medicine, medicine, Humans, Transplantation, Homologous, Child, Transplantation, business.industry, beta-Thalassemia, Hematopoietic Stem Cell Transplantation, Hematopoietic stem cell, Immunosuppression, Bayes Theorem, Hematology, medicine.disease, Sickle cell anemia, 3. Good health, surgical procedures, operative, medicine.anatomical_structure, Graft-versus-host disease, 030220 oncology & carcinogenesis, Cyclosporine, business, Goals, Immunosuppressive Agents, 030215 immunology
Abstract: Graft-versus-host disease (GVHD) is an important challenge and a major cause of morbidity and mortality in children after hematopoietic stem cell transplant (HSCT). Herein we report our institution's experience of goal-oriented Bayesian monitoring for cyclosporine (CsA) used alone as GVHD prophylaxis during the post-transplant period in pediatric patients with thalassemia major (TM) or sickle cell anemia (SCA) undergoing HLA-matched HSCT. We also studied evolution of chimerism. Twenty-six consecutive patients (SCA, 14; TM, 12) underwent matched sibling donor (MSD) HSCT from 2004 to 2014. All patients received a myeloablative conditioning regimen. GVHD prophylaxis consisted of 20 mg/kg antithymocyte globulin in the conditioning regimens and then CsA alone in the post-transplant period. Target CsA trough blood concentration (TBC) was 150 ± 20 ng/mL. At last follow-up, all patients were alive and free of disease, even in cases of mixed chimerism. Engraftment occurred in all patients. No patient developed grades II to IV acute GVHD, 4 patients developed acute grade I skin GVHD, and only 1 presented with chronic pulmonary GVHD. A better control of GVHD and immunosuppression by a strict monitoring of CsA TBC as described herein is promising and could play a crucial role. Further investigations are required, but this study opens new perspectives to improve survival and safety of HSCT from alternative donors in TM and SCA to levels compatible with that obtained with MSDs.
Published: 2020

16. Hemostasis in highlanders with excessive erythrocytosis at 5100 m: Preliminary data from the highest city of the world

Author: Philippe Connes, Julien V. Brugniaux, Benoit Champigneulle, Stéphane Doutreleau, Samuel Verges, Ivan Hancco, Aurélien Pichon, Emeric Stauffer, Yann Savina, Paul Robach, Hypoxie : Physiopathologie Respiratoire et Cardiovasculaire (HP2), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire 'Mobilité, Vieillissement, Exercice' (MOVE) (MOVE), Université de Poitiers, Centre Hospitalier Universitaire [Grenoble] (CHU), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Hospices Civils de Lyon (HCL), Hôpital de la Croix-Rousse [CHU - HCL], Laboratoire 'Mobilité, Vieillissement, Exercice' (MOVE), Ecole Nationale des Sports de Montagne (ENSM), and CCSD, Accord Elsevier
Subjects: Adult, Male, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Physiology, [SDV]Life Sciences [q-bio], Population, Polycythemia, Altitude Sickness, Hypoxic exposure, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Peru, medicine, Humans, education, High-altitude, Blood Coagulation, Hemostasis, education.field_of_study, Coagulation, Excessive erythrocytosis, business.industry, Altitude, General Neuroscience, Middle Aged, Effects of high altitude on humans, medicine.disease, [SDV] Life Sciences [q-bio], Chronic mountain sickness, 030228 respiratory system, Cardiology, Hemoglobin, business, 030217 neurology & neurosurgery, Highlanders
Abstract: International audience; Little is known about hemostasis modifications induced by chronic hypoxic exposure in high-altitude residents, especially in those who develop excessive erythrocytosis (EE, i.e. hemoglobin concentration ≥ 21 g·dL-1 in male and ≥ 19 g·dL-1 in female). The aim of this preliminary study was to assess coagulation alterations in highlanders with or without EE using simple hemostatic tests such as bleeding (BT) and clotting (CT) times. Eighty-one male (43 ± 7 years), permanent residents from La Rinconada (Peru), the highest city in the world (5,100-5,300 m), were evaluated. Thirty-six subjects (44 %) presented with EE. EE subjects compared to non-EE subjects had lower BT (3.6 ± 1.2 vs. 7.0 ± 1.9 min, p < 0.001) and CT (11.7 ± 1.7 vs. 15.1 ± 2.3 min, p < 0.001). These results support the notion that highlanders with EE are in a state of hypercoagulability and call for further hemostasis investigations in this population using more detailed hemostatic methods.
Published: 2020

17. Skeletal muscle of females and males with constitutional thinness: a low intramuscular lipid content and oxidative profile

Author: Bogdan Galusca, Angèle N. Merlet, Jörg Hager, Christophe Hourdé, Thierry Thomas, Julien Verney, David Thivel, Bruno Estour, Daniel Courteix, Bruno Pereira, Natacha Germain, Mélina Bailly, Léonard Féasson, Frédéric Costes, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Troubles du Comportement Alimentaire, Addictions et Poids Extêmes (TAPE - TCA), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Service Endocrinologie - Diabète et Maladies métaboliques [CHU Saint-Etienne], Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service Médecine du Sport et Explorations Fonctionnelles [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand, Service de Rhumatologie [CHU Saint-Etienne], Metabolic Health, Nestlé Research, EPFL Innovation Park, Lausanne, Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), TAPE laboratory [Saint-Étienne], Université Jean Monnet [Saint-Étienne] (UJM), Division of Endocrinology, Diabetes, Metabolism and Eating Disorders, CHU Saint-Étienne, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service de Médecine du Sport et des Explorations Fonctionnelles, Department of Rheumatology, Hôpital Nord, CHU Saint-Etienne, France. INSERM U1059, University of Lyon-Jean Monnet University, Saint-Étienne, and BAILLY, Mélina
Subjects: Male, Physiology, Endocrinology, Diabetes and Metabolism, Muscle Fibers, Skeletal, Skeletal muscle, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Weight Gain, Constitutional thinness, chemistry.chemical_compound, 0302 clinical medicine, Citrate synthase, Capillary supply, 0303 health sciences, Nutrition and Dietetics, biology, medicine.diagnostic_test, Glycogen, General Medicine, Adaptation, Physiological, medicine.anatomical_structure, Female, Underweight, medicine.symptom, Adult, medicine.medical_specialty, 030209 endocrinology & metabolism, Oxidative phosphorylation, Hyperphagia, Muscle glycogen, Young Adult, 03 medical and health sciences, Thinness, Physiology (medical), Internal medicine, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], medicine, Humans, Muscle, Skeletal, Triglycerides, 030304 developmental biology, Muscle biopsy, business.industry, Body Weight, Metabolism, Intramuscular triglycerides, Endocrinology, chemistry, Lipid content, Dietary Supplements, biology.protein, Oxidative capacity, Energy Intake, business
Abstract: Constitutional thinness (CT) is a nonpathological state of underweight. The current study aimed to explore skeletal muscle energy storage in individuals with CT and to further characterize muscle phenotype at baseline and in response to overfeeding. Thirty subjects with CT (15 females, 15 males) and 31 normal-weight control subjects (16 females, 15 males) participated in the study. Histological and enzymological analyses were performed on muscle biopsy specimens before and after overfeeding. In the skeletal muscle of CT participants compared with controls, we observed a lower content of intramuscular triglycerides for type I (−17%, p < 0.01) and type IIA (−14%, p < 0.05) muscle fibers, a lower glycogen content for type I (−6%, p < 0.01) and type IIA (−5%, p < 0.05) muscle fibers, a specific fiber-type distribution, a marked muscle hypotrophy (−20%, p < 0.001), a low capillary-to-fiber ratio (−19%, p < 0.001), and low citrate synthase activity (−18%, p < 0.05). In response to overfeeding, CT participants increased their intramuscular triglycerides content in type I (+10%, p < 0.01) and type IIA (+9%, p < 0.01) muscle fibers. CT individuals seem to present an unusual muscle phenotype and different adaptations to overfeeding compared with normal-weight individuals, suggesting a specific energy metabolism and muscle adaptations. ClinicalTrials.gov registration no. NCT02004821. Novelty Low intramuscular triglycerides and glycogen content in skeletal muscle of constitutionally thin individuals. Low oxidative capacity, low capillary supply, and fiber hypotrophy in skeletal muscle of constitutionally thin individuals. Increase in intramuscular triglycerides in constitutional thinness in response to overfeeding.
Published: 2020

18. Tensorpac: An open-source Python toolbox for tensor-based phase-amplitude coupling measurement in electrophysiological brain signals

Author: Juan L.P. Soto, Timothy Nest, Karim Jerbi, Aymeric Guillot, Robin A. A. Ince, Andrea Brovelli, Etienne Combrisson, Brovelli, Andrea, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université du Québec à Montréal = University of Québec in Montréal (UQAM), Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institute of Neurosciences and Psychology [Glasgow], University of Glasgow, Laboratorio de Polímeros, Pontificia Universidad Católica de Valparaíso (PUCV), Centre de Recherche et d'Innovation sur le Sport (EA647) (CRIS), Université de Lyon-Université de Lyon, Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-18-CE28-0016,CausaL,Architectures cognitives de l'apprentissage causale(2018), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de recherche en neurosciences de Lyon (CRNL), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Physiology, Computer science, Time Measurement, 0302 clinical medicine, Biology (General), computer.programming_language, Modulation, 0303 health sciences, Measurement, Ecology, Brain, Signal Processing, Computer-Assisted, Toolbox, Electrophysiology, Bioassays and Physiological Analysis, Brain Electrophysiology, Computational Theory and Mathematics, Computer engineering, Modeling and Simulation, Physical Sciences, Engineering and Technology, Software design, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Research Article, Computer and Information Sciences, QH301-705.5, Computation, Neurophysiology, Research and Analysis Methods, 03 medical and health sciences, Cellular and Molecular Neuroscience, Genetics, Humans, Tensor, [SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Spurious relationship, Molecular Biology, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Electrophysiological Techniques, Computational Biology, Biology and Life Sciences, Signal Bandwidth, Python (programming language), Probability Theory, Probability Distribution, Computing Methods, Electrophysiological Phenomena, Visualization, 030104 developmental biology, Signal Processing, computer, Software, Mathematics, 030217 neurology & neurosurgery, Neuroscience, Information integration
Abstract: Despite being the focus of a thriving field of research, the biological mechanisms that underlie information integration in the brain are not yet fully understood. A theory that has gained a lot of traction in recent years suggests that multi-scale integration is regulated by a hierarchy of mutually interacting neural oscillations. In particular, there is accumulating evidence that phase-amplitude coupling (PAC), a specific form of cross-frequency interaction, plays a key role in numerous cognitive processes. Current research in the field is not only hampered by the absence of a gold standard for PAC analysis, but also by the computational costs of running exhaustive computations on large and high-dimensional electrophysiological brain signals. In addition, various signal properties and analyses parameters can lead to spurious PAC. Here, we present Tensorpac, an open-source Python toolbox dedicated to PAC analysis of neurophysiological data. The advantages of Tensorpac include (1) higher computational efficiency thanks to software design that combines tensor computations and parallel computing, (2) the implementation of all most widely used PAC methods in one package, (3) the statistical analysis of PAC measures, and (4) extended PAC visualization capabilities. Tensorpac is distributed under a BSD-3-Clause license and can be launched on any operating system (Linux, OSX and Windows). It can be installed directly via pip or downloaded from Github (https://github.com/EtienneCmb/tensorpac). By making Tensorpac available, we aim to enhance the reproducibility and quality of PAC research, and provide open tools that will accelerate future method development in neuroscience.
Published: 2020

19. Central fatigue aetiology in prolonged trail running races

Author: Callum G. Brownstein, Thibault Besson, Nicolas Royer, Vincent Martin, Loïc Espeit, Guillaume Y. Millet, Thomas Lapole, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne (UCA)-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: medicine.medical_specialty, Physiology, Tibial nerve stimulation, 030204 cardiovascular system & hematology, Plantar flexion, Running, 03 medical and health sciences, 0302 clinical medicine, Voluntary contraction, Physical medicine and rehabilitation, Physiology (medical), [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, Mont blanc, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Nutrition and Dietetics, business.industry, Electromyography, General Medicine, Torque, Muscle Fatigue, Etiology, Physical Endurance, H-reflex, business, 030217 neurology & neurosurgery, Gastrocnemius medialis, Muscle Contraction
Abstract: NEW FINDINGS What is the central question of this study? Are spinal and/or supraspinal perturbations implicated in central fatigue induced in the plantar flexor muscles following prolonged trail running races? What is the main finding and its importance? The study confirmed the presence of central fatigue following various trail running distances from 40 to 170 km. The reduction in the V-wave in conjunction with the lack of change in the H-reflex suggests that a major component of this central fatigue may arise from supraspinal mechanisms in the plantar flexor muscles. ABSTRACT Trail running races are known to induce considerable impairments in neuromuscular function of which central mechanisms are a substantial component. However, the loci of this central fatigue (i.e. supraspinal and/or spinal) is not well identified. The aim of this study was to better understand central fatigue aetiology induced in the plantar flexor muscles by various trail running distances from 40 to 170 km. Eighteen runners participated in the study and neuromuscular function of their plantar flexors was tested before (PRE) and after (POST) various races during the Ultra-Trail du Mont Blanc. Neuromuscular function was evaluated with voluntary and evoked contractions using electrical tibial nerve stimulation. H-reflex and V-wave responses were also measured during submaximal and maximal voluntary contraction, respectively. Reductions in maximal voluntary contraction torque (-29%; P
Published: 2020

20. 3D Visualization of Body Motion in Speed Climbing

Author: Franck Quaine, Lionel Reveret, Pierre Legreneur, Sylvain Chapelle, Calcul des Variations, Géométrie, Image (CVGI), Laboratoire Jean Kuntzmann (LJK), Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Fédération Française de la Montagne et de l'Escalade, GIPSA - Analyse et Modification de l'homme en mouvement : biomécanique, cognition, vocologie (GIPSA-MOVE), GIPSA Pôle Sciences des Données (GIPSA-PSD), Grenoble Images Parole Signal Automatique (GIPSA-lab), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Grenoble Images Parole Signal Automatique (GIPSA-lab), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Motion analysis, lcsh:BF1-990, Tracking (particle physics), biomechanics, 050105 experimental psychology, Motion (physics), [SPI]Engineering Sciences [physics], 03 medical and health sciences, 0302 clinical medicine, video analysis, Psychology, 0501 psychology and cognitive sciences, Computer vision, Polygon mesh, General Psychology, Original Research, Plane (geometry), business.industry, motion analysis, 05 social sciences, speed climbing, Visualization, lcsh:Psychology, 3D visualization, center of mass, Climbing, Artificial intelligence, business, 030217 neurology & neurosurgery, Energy (signal processing)
Abstract: International audience; Speed climbing involves an optimization of the velocity of the ascent and the trajectory path during performance. Consequently, any amount of energy spent in the two other directions than vertical, namely the lateral direction and the direction perpendicular to the wall plane, is a potential loss of performance. To assess this principle, we present a study on 3D motion analysis and its 3D visualization for a subject during a speed climbing performance. The fundamentals of geometrical measurement in 3D require to integrate multiple 2D cues, at least two, in order to extract 3D information. First results with two drones following an athlete's ascent show that a 3D velocity profile can be provided from the tracking of a marker on the harness, pointing critical phases in the ascent where the vertical speed is not dominant any more. We further investigate 3D motion of full body using markerless video-based tracking. Our approach is based on a full body 3D avatar model of the climber, represented as a 3D mesh. This model and its deformation are learned in a laboratory studio. The learning needs to be done only once. Result is a manifold embedding of the 3D mesh and its deformations, which can be used afterwards to perform registration onto video of performance of speed climbing. The results of the tracking is an inference of the 3D mesh aligned onto videos of speed climbing performance. From this 3D mesh, we deduce an estimation of the center of mass (COM). We show that this estimation from 3D mesh differs from the usual approximation of the COM as a marker on the harness. In particular, the 3D mesh COM takes into account the whole body movement such as the influence of the limbs which is not detected by a marker on the harness.
Published: 2020

21. Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Singlebla(CTX-M-1)/IncI1/ST3 Plasmid Among Humans and Wild Animals

Author: Stephanie Guyomard-Rabenirina, Yann Reynaud, Matthieu Pot, Emmanuel Albina, David Couvin, Celia Ducat, Gaëlle Gruel, Severine Ferdinand, Pierre Legreneur, Simon Le Hello, Edith Malpote, Syndia Sadikalay, Antoine Talarmin, Sebastien Breurec, Unité Transmission, Réservoir et Diversité des Pathogènes [Pasteur Guadeloupe, France] (TReD-Path), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université de Caen Normandie (UNICAEN), Normandie Université (NU), Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris] (IP), CHU Pointe-à-Pitre/Abymes [Guadeloupe], Université des Antilles - UFR des sciences médicales Hyacinthe Bastaraud (UA UFR SM), Université des Antilles (UA), Clinical Investigation and Epidemiology Center, INSERM 802, Guadeloupe, CHU Fattouma Bourguiba [Monastir] (HFB), This work has been supported by a FEDER grant, financed by the European Union and Guadeloupe Region [Programme Operationnel FEDER-Guadeloupe-Conseil Regional 2014-2020, Grant number 2018-FED-1084 (MALIN 2, https://www.projetmalin.fr)].Publisher, European Project: 2015-FED-192,FEDER-Guadeloupe, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Pasteur [Paris], LESUR, Hélène, and Programme Opérationnel FEDER-Guadeloupe-Conseil Régional 2014-2020 - FEDER-Guadeloupe - 2015-FED-192 - INCOMING
Subjects: Microbiology (medical), Veterinary medicine, medicine.drug_class, [SDV]Life Sciences [q-bio], Antibiotics, Cephalosporin, Wildlife, lcsh:QR1-502, extended-spectrum beta-lactamase, Biology, medicine.disease_cause, Microbiology, lcsh:Microbiology, 03 medical and health sciences, Antibiotic resistance, Plasmid, plasmid, wild animals, medicine, Escherichia coli, antimicrobial resistance, 030304 developmental biology, West indies, Original Research, 0303 health sciences, 030306 microbiology, 3. Good health, [SDV] Life Sciences [q-bio], Carriage
Abstract: Limited data are available on the contribution of wildlife to the spread of antibacterial resistance. We determined the prevalence of resistance to antibiotics in Escherichia coli isolates collected from wild animals in 2013 and 2014 and the genetic basis for resistance to third-generation cephalosporin in Guadeloupe. We recovered 52 antibiotic-resistant (AR) E. coli strains from 48 of the 884 (5.4%) wild animals tested (46 iguanas, 181 birds, 289 anoles, and 368 rodents at 163 sampling sites). Rodents had higher rates of carriage (n = 38, 10.3%) than reptiles and birds (2.4% and 1.1%, respectively, p < 0.001). A significant association (p < 0.001) was found between the degree of anthropization and the frequency of AR E. coli carriage for all species. The carriage rate of ciprofloxacin- and cefotaxime-resistant isolates was 0.7% (6/884) and 1.5% (13/884), respectively. Most (65.4%) AR E. coli were multi-drug resistant, and the prevalence of extended-spectrum beta-lactamase (ESBL)-producing E. coli was low (n = 7, 0.8%) in all species. Eight ESBL-producing E. coli were recovered, two genetically unrelated isolates being found in one bird. These isolates and 20 human invasive ESBL E. coli isolates collected in Guadeloupe during the same period were investigated by whole genome sequencing. blaCTX–M–1 was the only ESBL gene shared by three animal classes (humans, n = 2; birds, n = 2; rodents, n = 2). The blaCTX–M–1 gene and most of the antimicrobial resistance genes were present in a large conjugative IncI1 plasmid that was highly similar (>99% nucleotide identity) to ESBL-carrying plasmids found in several countries in Europe and in Australia. Although the prevalence of ESBL-producing E. coli isolates was very low in wild animals, it is of concern that the well-conserved IncI1 plasmid-carrying blaCTX–M–1 is widespread and occurs in various E. coli strains from animals and humans.
Published: 2020

22. Impact of running an ultramarathon on neuromuscular fatigue: effects of sex and distance

Author: Besson, Thibault, Parent, Audrey, Brownstein, Callum, Espeit, Loïc, Lapole, Thomas, Vincent, Martin, Royer, Nicolas, Rimaud, Diana, Sabater Pastor, Frederic, Singh, Benjamin, Varesco, Giorgio, Rossi, Jérémy, Temesi, John, Millet, Guillaume, Martin, Vincent, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université du Québec à Montréal = University of Québec in Montréal (UQAM), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), University of Northumbria at Newcastle [United Kingdom], and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Abstract: International audience; Few studies have assessed sex differences in neuromuscular fatigue after prolonged running. Compared to men, women exhibit less peripheral fatigue in plantar flexor muscles (PF) after running a 110-km-mountain-ultramathon [1]. The purpose of the present study was to further characterize sex differences by examining various running distances. Thirty-six runners completed the study: 18 men (36 ± 8 years, 72.5 ± 9.6 kg) and 18 women (36 ± 8 years, 59.1 ± 5.8 kg). Men and women were matched by relative performance (i.e. percent of winning time of their sex category). Participants completed various races of the Ultra-Trail du Mont-Blanc® ranging from 40 km with 2,300 m of positive elevation change to 170 km with 10,000 m of positive elevation change. One month before the race, participants visited the lab to be familiarized with electrical stimulation on both knee extensor (KE) and PF muscles. Neuromuscular function was tested before and after each race. The testing protocol consisted of a standardized warm-up followed by the assessment of maximal voluntary contractions (MVCs), maximal voluntary activation (superimposed 100 Hz doublet) and contractile properties (potentiated 100 Hz doublet, potentiated 10 Hz doublet and potentiated single twitch (Pt)). For analysis, participants were further subdivided into two groups of 18 runners by distance of race completed (SHORT < 100 km vs LONG ≥ 100 km). A factorial ANOVA with deficit (in percentage of PRE value) as a dependent factor and with sex and distance as between-subject factors was used for the analysis.MVC loss was greater after LONG than SHORT in both KE (p
Published: 2020

23. Physical activity preferences before and after participation in a 6‐month physical activity intervention among women with metastatic breast cancer

Author: Béatrice Fervers, Magali Morelle, Olivia Pérol, Lidia Delrieu, Marina Touillaud, Thomas Bachelot, Christine M. Friedenreich, Pierre-Etienne Heudel, Jeff K. Vallance, Vincent Pialoux, Olivier Tredan, Armelle Dufresne, Centre Léon Bérard [Lyon], Groupe d'analyse et de théorie économique (GATE Lyon Saint-Étienne), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université Lumière - Lyon 2 (UL2)-École normale supérieure - Lyon (ENS Lyon), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Division of Population Health and Information, Alberta Cancer Board, Service d'Oncologie Médicale [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Oncogénèse et progression tumorale, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Médecine Nucléaire, École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Service d'oncologie Médicale, Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Hôpital Jean Minjoz, Groupe d'Analyse et de Théorie Economique Lyon - Saint-Etienne (GATE Lyon Saint-Étienne), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Counseling, medicine.medical_specialty, Physical activity, Bone Neoplasms, Breast Neoplasms, Cancer Care Facilities, Fitness Centers, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Intervention (counseling), Secondary analysis, medicine, Humans, Neoplasm Metastasis, Exercise, ComputingMilieux_MISCELLANEOUS, Aged, Oncologists, Physical activity interventions, business.industry, Brain Neoplasms, Activity tracker, Patient Preference, Middle Aged, medicine.disease, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, Metastatic breast cancer, Physical activity level, Exercise Therapy, Oncology, 030220 oncology & carcinogenesis, Physical therapy, Female, business, Social vulnerability
Abstract: Objective This secondary analysis of the ABLE Trial (ClinicalTrials.gov NCT03148886) aimed to assess physical activity preferences before and after a 6-month physical activity intervention for women recently diagnosed with metastatic breast cancer and to investigate demographic and clinical correlates of these preferences. Methods Forty-nine patients participated in the ABLE Trial, a single-arm, unsupervised 6-month physical activity intervention with activity trackers. At baseline and 6 months, physical activity preferences, physical activity level, clinical variables, demographics and social vulnerability were assessed. Results At baseline, 49 participants were included, among whom 85% were interested in receiving physical activity counselling and 89% were interested in following a physical activity programme designed for metastatic breast cancer. At the end of the study, more participants preferred practising in a community fitness centre (66%) rather than at home (19% vs. 44% at baseline, p = .03). A higher social vulnerability score and not being treated by chemotherapy at baseline were significantly associated with lower desire to receive physical activity counselling (p = .01 and p = .04 respectively). Conclusions This study will help design future studies within patients with metastatic breast cancer in accordance with their preferences. Designing tailored physical activity interventions according to the participant's preferences may be one key to success for adherence.
Published: 2020

24. Cost-effectiveness of an exercise and nutritional intervention versus usual nutritional care during adjuvant treatment for localized breast cancer: the PASAPAS randomized controlled trial

Author: Séverine Guillemaut, Anne-Sophie Kempf-Lépine, Eric Reynes, Lionel Perrier, Cédric Baudinet, Sophie E. Berthouze, Béatrice Fervers, Dominik Heinz, Frédéric Gomez, Renaud Meyrand, Marina Touillaud, Julien Carretier, Thierry Philip, Aude-Marie Foucaut, Olivier Tredan, David Pérol, Patrick Bachmann, Magali Morelle, Centre Léon Bérard [Lyon], Groupe d'analyse et de théorie économique (GATE Lyon Saint-Étienne), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université Lumière - Lyon 2 (UL2)-École normale supérieure - Lyon (ENS Lyon), Laboratoire Educations et Pratiques de Santé (LEPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Sorbonne Paris Cité (USPC)-Université Paris 13 (UP13), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire sur les Vulnérabilités et l'Innovation dans le Sport (EA 7428) (L-VIS), Université de Lyon-Université de Lyon, Département cancer environnement (Centre Léon Bérard - Lyon), Groupe d'Analyse et de Théorie Economique Lyon - Saint-Etienne (GATE Lyon Saint-Étienne), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, medicine.medical_specialty, Adolescent, Cost effectiveness, Cost-Benefit Analysis, Breast Neoplasms, Body fat percentage, law.invention, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Breast cancer, Randomized controlled trial, law, medicine, Humans, 030212 general & internal medicine, Aerobic capacity, ComputingMilieux_MISCELLANEOUS, Aged, 2. Zero hunger, Nutritional Support, business.industry, Cost-effectiveness analysis, Middle Aged, medicine.disease, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, Exercise Therapy, 3. Good health, Clinical trial, Oncology, Chemotherapy, Adjuvant, 030220 oncology & carcinogenesis, Physical therapy, Female, business, Body mass index
Abstract: We undertook a cost-effectiveness analysis (CEA) to compare an exercise and nutritional program with the usual nutritional care concomitant to adjuvant chemotherapy in localized breast cancer patients. The CEA was designed as part of the interventional, controlled, randomized, single-center, open-label PASAPAS study. Breast cancer patients receiving first-line adjuvant chemotherapy at a French Comprehensive Cancer Center were randomized 2:1 to a 6-month exercise program of supervised indoor and outdoor group sessions in addition to usual nutritional care (exercise arm) or a usual nutritional care group receiving dietary and physical activity counseling (control arm). Costs were assessed from the French national insurance perspective (in Euros, 2012). Incremental cost-effectiveness ratios (ICERs) were calculated for four criteria: body mass index, waist circumference, body fat percentage, and estimated aerobic capacity. Uncertainty around the ICERs was captured by a probabilistic analysis using a non-parametric bootstrap method. The analysis was based on 60 patients enrolled between 2011 and 2013. Average intervention costs per participant were €412 in the exercise arm (n = 41) and €117 (n = 19) in the control arm. Total mean costs were €17,344 (standard deviation 9,928) and €20,615 (standard deviation 14,904), respectively, did not differ significantly (p = 0.51). The 6-month exercise program was deemed to be cost-effective compared with usual care for the estimated aerobic capacity. Multicenter randomized studies with long-term costs and outcomes should be done to provide additional evidence. Clinical trial: The PASAPAS study is registered under ClinicalTrials.gov. Trial registration ID: NCT01331772.
Published: 2020

25. Dual mobility cups for total hip arthroplasty: tips and tricks

Author: Thomas Neri, Cécile Batailler, Antonio Klasan, Rémi Philippot, Bertrand Boyer, Frédéric Farizon, Sébastien Lustig, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Service de Chirurgie Orthopédique [Centre Albert Trillat], Centre Albert Trillat [Hôpital de la Croix-Rousse - HCL], Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL)-Hôpital de la Croix-Rousse [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Royal North Shore Hospital (RNSH), Hospices Civils de Lyon (HCL), Cadic, Ifsttar, Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre Ingénierie et Santé (CIS-ENSMSE), and Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: medicine.medical_specialty, [SDV.MHEP.CHI] Life Sciences [q-bio]/Human health and pathology/Surgery, tricks, Context (language use), tips, DUAL MOBILITY CUP, Review Article, [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery, 03 medical and health sciences, 0302 clinical medicine, lcsh:Orthopedic surgery, Medicine, Orthopedics and Sports Medicine, Complication rate, Medical physics, 030212 general & internal medicine, SURGICAL TECHNIQUE, 030222 orthopedics, [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, business.industry, Implant compatibility, TOTAL HIP ARTHROPLASTY, Dual mobility, Special Issue: "HIP and KNEE Replacement" Guest Editors: C Batailler, S Lustig, J Caton, lcsh:RD701-811, [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system, Orthopedic surgery, TIP, Surgery, business, TRICK, Total hip arthroplasty
Abstract: Since its creation in 1974, the Dual Mobility Cup (DMC) has been gaining in popularity, especially in the past decade. This intensive use could lead to inappropriate use and consequently to an increased complication rate. Compliance with conceptual requirements and surgical techniques will prevent the occurrence of complications that can be wrongly attributed to implants. In this context, we feel that it is essential to share our tips and tricks as well as an overview and an explanation of common errors, based on more than 45 years of clinical and research experience. From basic principles, including indications, implant choice and implant compatibility, to surgical tips, in this article orthopedic surgeons will find a practical overview of DMC in order to use it safely and with confidence.
Published: 2020

26. Muscle alterations induced by electrostimulation are lower at short quadriceps femoris length

Author: Julien Gondin, Alexandre Fouré, Augustin C. Ogier, David Bendahan, Maxime Guye, Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), APHM, Hôpital Universitaire Timone, Centre d'Exploration Métabolique par Résonance Magnétique (CEMEREM), 13005 MARSEILLE, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Images et Modèles (I&M), Laboratoire d'Informatique et Systèmes (LIS), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut NeuroMyoGène (INMG), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Exploration Métabolique par Résonance Magnétique [Hôpital de la Timone - APHM] (CEMEREM), Hôpital de la Timone [CHU - APHM] (TIMONE)-Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Ben Dahan, David
Subjects: Adult, Male, medicine.medical_specialty, Physiology, Single group, Stimulation, Isometric exercise, Muscle damage, Thigh, Quadriceps Muscle, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Physiology (medical), Internal medicine, Isometric Contraction, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, Orthopedics and Sports Medicine, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Knee extensors, business.industry, Public Health, Environmental and Occupational Health, 030229 sport sciences, General Medicine, Electric Stimulation, Intensity (physics), medicine.anatomical_structure, Transverse Relaxation Time, Cardiology, Female, business, 030217 neurology & neurosurgery
Abstract: International audience; Purpose: This study aimed at determining through MRI investigations, force and soreness assessments whether the modulation of muscle length is a relevant strategy for minimising neuromuscular electrical stimulation (NMES)-induced muscle damage in young healthy participants.Methods: Comparison of 2 NMES sessions (40 isometric electrically-evoked contractions of the knee extensors) was randomly performed on 1 knee flexed at 50° (short muscle length) and the contralateral at 100° (long muscle length) in a single group of healthy participants. Indirect markers of muscle damage including changes in maximal voluntary isometric contraction (MVC) force, muscle volume and transverse relaxation time (T2) were measured before, 2 days (D2), 4 days (D4) and 7 days (D7) after the NMES sessions in each limb of the ten participants.Results: Although stimulation intensity was similar during the NMES session on both limbs, significantly lower force production was recorded at long muscle length (peak at 30 ± 5% MVC force) as compared to short muscle length (peak at 61 ± 12% MVC force). In the following days, MVC force at long muscle length was decreased from D2 to D7, whereas no significant change occurred at short muscle length. Increases in muscle volume and T2 were found at each time point in stimulated muscles at long muscle length, whereas no change was found at short muscle length.Conclusion: For the same stimulation intensity, NMES-induced isometric contractions generate higher knee extension force output and result in lower muscle tissues alterations that could be related to a lower intramuscular shear strain when exercise is performed at short muscle length.
Published: 2019

27. Rituximab is an effective treatment in patients with pemphigus vulgaris and demonstrates a steroid‐sparing effect

Author: Bruno Sassolas, Frédéric Caux, Olivier Dereure, Saskia Ingen-Housz-Oro, E. Csinady, Estelle Houivet, D.M. Chen, Jean-David Bouaziz, C. Prost‐Squarcioni, S. Duvert-Lehembre, Sébastien Calbo, P. Bernard, Pierre Vabres, Nicolas Dupin, L. Gearhart, Pascal Joly, Laurent Machet, Jacques Benichou, A. Odueyungbo, Carle Paul, Philippe Musette, Gaëlle Quéreux, Sébastien Debarbieux, Alain Dupuy, Catherine Picard-Dahan, Nathalie Beneton, Emmanuel Delaporte, Maud Maho-Vaillant, Michel D'Incan, Marie-Laure Golinski, Denis Jullien, Frédérique Caillot, P.B. Lehane, Marie Aleth Richard, Bruno Labeille, Laurent Misery, Christophe Bedane, M. Alexandre, Vivien Hébert, Martine Avenel-Audran, M. Cheu, Marie Beylot-Barry, Service de Dermatologie [Rouen], Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU), Physiopathologie, Autoimmunité, maladies Neuromusculaires et THErapies Régénératrices (PANTHER), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de biostatistiques [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), University Hospital of St-Etienne, Department of Dermatology, Service de dermatologie [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'oncologie [Hôpital privé Toulon Hyères : Sainte Marguerite], Hôpital privé Toulon Hyères : Sainte Marguerite, Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay, Hôpital Henri Mondor, Equipe GAD (LNC - U1231), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Hôpital Claude Huriez [Lille], CHU Lille, Service de dermatologie [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Service de dermatologie [CH Le Mans], Centre Hospitalier Le Mans (CH Le Mans), Institut Laue-Langevin (ILL), Calcul Thermodynamique, Hôpital de la Cavale Blanche - CHRU Brest (CHU - BREST ), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre Hospitalier Universitaire de Reims (CHU Reims), Immuno-Régulation dans les Maladies Auto-Immunes Inflammatoires et le Cancer - EA 7509 (IRMAIC), Université de Reims Champagne-Ardenne (URCA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Université Paris Diderot - Paris 7 (UPD7)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, ILL, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Hôpital Charles Nicolle [Rouen], Service de Dermatologie [CHU Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: medicine.medical_specialty, Prednisolone, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Dermatology, Gastroenterology, law.invention, Antibodies, Monoclonal, Murine-Derived, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, Prednisone, Internal medicine, Humans, Immunologic Factors, Medicine, In patient, Adverse effect, ComputingMilieux_MISCELLANEOUS, business.industry, Standard treatment, Pemphigus vulgaris, Original Articles, medicine.disease, Clinical Trial, 3. Good health, Treatment Outcome, [SDV.IMM]Life Sciences [q-bio]/Immunology, Rituximab, business, Adjuvant, Immunosuppressive Agents, Pemphigus, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, medicine.drug
Abstract: Summary Background Corticosteroids (CS) with or without adjuvant immunosuppressant agents are standard treatment for pemphigus vulgaris (PV). The efficacy of adjuvant therapies in minimizing steroid‐related adverse events (AEs) is unproven. Objectives To utilize data collected in a French investigator‐initiated, phase III, open‐label, randomized controlled trial to demonstrate the efficacy and safety of rituximab and seek approval for its use in PV. Methods This was an independently conducted post hoc analysis of the moderate‐to‐severe PV subset enrolled in the Ritux 3 study. Patients were randomized to rituximab plus 0·5 or 1·0 mg kg−1 per day prednisone tapered over 3 or 6 months, or 1·0 or 1·5 mg kg−1 per day prednisone alone tapered over 12 or 18 months, respectively (according to disease severity). The primary end point was complete remission at month 24 without CS (CRoff) for ≥ 2 months, and 24‐month efficacy and safety results were also reported. Results At month 24, 34 of 38 patients (90%) on rituximab plus prednisone achieved CRoff ≥ 2 months vs. 10 of 36 patients (28%) on prednisone alone. Median total cumulative prednisone dose was 5800 mg in the rituximab plus prednisone arm vs. 20 520 mg for prednisone alone. Eight of 36 patients (22%) who received prednisone alone withdrew from treatment owing to AEs; one rituximab‐plus‐prednisone patient withdrew due to pregnancy. Overall, 24 of 36 patients (67%) on prednisone alone experienced a grade 3/4 CS‐related AE vs. 13 of 38 patients (34%) on rituximab plus prednisone. Conclusions In patients with moderate‐to‐severe PV, rituximab plus short‐term prednisone was more effective than prednisone alone. Patients treated with rituximab had less CS exposure and were less likely to experience severe or life‐threatening CS‐related AEs. What's already known about this topic? Pemphigus vulgaris (PV) is the most common type of pemphigus.Corticosteroids, a standard first‐line treatment for PV, have significant side‐effects.Although their effects are unproven, adjuvant corticosteroid‐sparing agents are routinely used to minimize steroid exposure and corticosteroid‐related side‐effects.There is evidence that the anti‐CD20 antibody rituximab is effective in the treatment of patients with severe recalcitrant pemphigus and in patients with newly diagnosed pemphigus. What does this study add? This study provides a more detailed analysis of patients with PV enrolled in an investigator‐initiated trial.Rituximab plus prednisone had a steroid‐sparing effect and more patients achieved complete remission off prednisone.Fewer patients experienced grade 3 or grade 4 steroid‐related adverse events than those on prednisone alone.This collaboration between academia and industry, utilizing independent post hoc analyses, led to regulatory authority approvals of rituximab in moderate‐to‐severe PV., Linked Comment: Scorer et al. Br J Dermatol 2020; 182:1078–1079.
Published: 2019

28. Areas of muscle tissue alteration can differ from activated regions during electrically-induced isometric contractions

Author: Fouré, Alexandre, Le Troter, Arnaud, Ogier, Augustin C., David, Bendahan, Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Images et Modèles (I&M), Laboratoire d'Informatique et Systèmes (LIS), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

29. Comparison of Active and Sedentary Bout Lengths in Normal and Overweight Adults using eMouverecherche

Author: Rousset, Sylvie, Ludivine, Paris, Romain, Guidoux, Damien, Saboul, Martine, Duclos, Yves, Boirie, Sylvie, Rousset, Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), IP Leanware, ALMERYS, Orange Business Services, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service Médecine du Sport et des Explorations Fonctionnelles, CHU Clermont-Ferrand, Service de Nutrition Clinique [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service Médecine du Sport et Explorations Fonctionnelles [CHU Clermont-Ferrand], and CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand]
Subjects: Accelerometer, Physical activity bout, Sedentary bout, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Overweight adults, human activities, eMouveRecherche application, ComputingMilieux_MISCELLANEOUS, Normal-weight adults
Abstract: International audience; BackgroundPhysical inactivity and long sedentary time are common in obese people. The eMouveRecherche application was developed to provide accurate minute-by-minute classification of physical activity in light, moderate, vigorous intensity and sedentary bouts. The aim was to compare the frequency and length of bouts in Normal-Weight (NW) and Overweight (OW) adults.MethodsFifty-seven adult participants either normal weight or overweight wore a smartphone with the eMouve application for the entire waking period of the day. The continuous 1-5, 5-15, 15-30, 30-60 and higher than 60-minute bouts for each behavior were counted.ResultsThe total number of bouts was higher in NW than in OW (12.4 vs. 9.8 bouts.h-1, p < 0.001). The breakdown of immobile and active bouts according to their length was different in the two groups. The NW had a significantly higher percentage of brief immobile bouts (1-5 min) (65.2% vs. 49.7%), while OW had a significantly higher percentage of 5-15 min (26.8% vs. 19.1%) and 15-30 min sedentary bouts (11.8% vs. 8.0%). The 1-5 min bouts of light-intensity activity were statistically more frequent in OW (93.6% vs. 83.5%), whereas bouts of 5-15 min (15.1% vs. 6.4%) and 15-30 min bouts (1.3% vs. 0%) were more common in NW.ConclusionThe frequency of both immobile and light-intensity activity bouts was lower in OW, whereas the duration of bouts was respectively longer for immobile behavior and shorter for light-intensity activity, resulting in a continuous sedentary pattern with few active breaks. The overweight appears to be a barrier to the spontaneous practice of light-intensity physical activity.
Published: 2019

30. A novel segmentation framework dedicated to the follow‐up of fat infiltration in individual muscles of patients with neuromuscular disorders

Author: C. Michel, Alexandre Fouré, David Bendahan, Arend Heerschap, Arnaud Le Troter, Linda Heskamp, Marc-Emmanuel Bellemare, Augustin C. Ogier, Images et Modèles (I&M), Laboratoire d'Informatique et Systèmes (LIS), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Radboud University Medical Center [Nijmegen], Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Intraclass correlation, Fat infiltration, Image registration, Muscle volume, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, All institutes and research themes of the Radboud University Medical Center, 0302 clinical medicine, Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15], Quantitative assessment, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Humans, Medicine, Radiology, Nuclear Medicine and imaging, Segmentation, ComputingMilieux_MISCELLANEOUS, Leg, Reproducibility, business.industry, Reproducibility of Results, Image segmentation, Magnetic Resonance Imaging, Thigh, business, Algorithms, 030217 neurology & neurosurgery, Follow-Up Studies, Biomedical engineering
Abstract: International audience; Purpose: To propose a novel segmentation framework that is dedicated to the follow-up of fat infiltration in individual muscles of patients with neuromuscular disorders.Methods: We designed a semi-automatic segmentation pipeline of individual leg muscles in MR images based on automatic propagation through nonlinear registrations of initial delineation in a minimal number of MR slices. This approach has been validated for the segmentation of individual muscles from MRI data sets, acquired over a 10-month period, from thighs and legs in 10 patients with muscular dystrophy. The robustness of the framework was evaluated using conventional metrics related to muscle volume and clinical metrics related to fat infiltration.Results: High accuracy of the semi-automatic segmentation (mean Dice similarity coefficient higher than 0.89) was reported. The provided method has excellent reliability regarding the reproducibility of the fat fraction estimation, with an average intraclass correlation coefficient score of 0.99. Furthermore, the present segmentation framework was determined to be more reliable than the intra-expert performance, which had an average intraclass correlation coefficient of 0.93.Conclusion: The proposed framework of segmentation can successfully provide an effective and reliable tool for accurate follow-up of any MRI biomarkers in neuromuscular disorders. This method could assist the quantitative assessment of muscular changes occurring in such diseases.
Published: 2019

31. Motor imagery ability of patients with lower-limb amputation: exploring the course of rehabilitation effects

Author: Elodie Saruco, Catherine Mercier, Anne Durand, Philip L. Jackson, Aymeric Guillot, Franck Di Rienzo, Arnaud Saimpont, Francine Malouin, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut de Réadaptation en Déficience Physique de Québec, Biostatistiques santé, Département biostatistiques et modélisation pour la santé et l'environnement [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Centre Interdisciplinaire de Recherche en Réadaptation et Intégration Sociale (CIRRIS), Université Laval [Québec] (ULaval), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Male, 030506 rehabilitation, medicine.medical_specialty, Longitudinal study, Imagery, Psychotherapy, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Population, Physical Therapy, Sports Therapy and Rehabilitation, 03 medical and health sciences, 0302 clinical medicine, Physical medicine and rehabilitation, Motor imagery, Amputees, Lower limb amputation, medicine, Humans, Longitudinal Studies, education, ComputingMilieux_MISCELLANEOUS, Aged, education.field_of_study, Rehabilitation, business.industry, Recovery of Function, Middle Aged, Lower Extremity, Amputation, Exercise Test, Female, Observational study, 0305 other medical science, business, 030217 neurology & neurosurgery, Independent living
Abstract: Background Amputation of a lower-limb results in a severe decrease of functional mobility that deeply alters independent living. Motor imagery (MI) refers to the mental representation of an action without engaging its actual execution. The repetitive use of MI has been shown to contribute to promote motor recovery and phantom-limb pain alleviation. Aim Given the importance of invoking accurate images to benefit from MI practice, and considering the link between motor capacities and MI, the present study investigated the effect of a rehabilitation program on MI ability in patients with lower-limb amputation. Design Observational and longitudinal study. Setting Patients recruited from the Amputation program at the Institut de Readaptation en Deficience Physique de Quebec (IRDPQ), in Quebec City, Canada. Population Patients with trans-tibial or transfemoral amputation, following a rehabilitation program as outpatients. Methods MI ability of the patients was measured at three different time points along the course of physical therapy. Results The data revealed a positive effect of the rehabilitation program on MI accuracy of locomotor tasks, and greater MI vividness and accuracy for single-joint movements that patients were still able to physically perform. Conclusions These findings suggest that MI abilities and actual motor performance are mirrored in a congruent fashion. Clinical rehabilitation impact Therapists should consider this critical aspect when including MI practice in rehabilitation programs among patients with lower-limb amputation.
Published: 2019

32. Réduction de modèle numérique pour la prévision de la pression d'interface appliquée par les bandages de compression sur la jambe inférieure

Author: Jérôme Molimard, Reynald Convert, Pierre Badel, Fanette Chassagne, Pascal Giraux, INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Thuasne, Université Jean Monnet - Faculté de Médecine, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Molimard, Jérôme, Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Ingénierie et Santé (CIS-ENSMSE), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University Jean Monnet
Subjects: Adult, Male, Patient-Specific Modeling, Materials science, Compression Bandage, Friction, Finite Element Analysis, 0206 medical engineering, Biomedical Engineering, 02 engineering and technology, 030204 cardiovascular system & hematology, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, [SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Compression Bandages, model reduction, Pressure, Humans, Vascular Diseases, Lymphatic Diseases, Leg, Computer simulation, Tension (physics), [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Mechanics, Middle Aged, 020601 biomedical engineering, Finite element method, Pressure measurement, interface pressure, Female, Reduction (mathematics), Parametrization, Finite element simulation, Bandage
Abstract: International audience; Objective: To develop a new method for the prediction of interface pressure applied by medical compression bandages. Methods: A finite element simulation of bandage application was designed, based on patient-specific leg geometries. For personalized interface pressure prediction, a model reduction approach was proposed, which included the parametrization of the leg geometry. Pressure values computed with this reduced model were then confronted to experimental pressure values. Results: The most influencing parameters were found to be the bandage tension, the skin-to-bandage friction coefficient and the leg morphology. Thanks to the model reduction approach, it was possible to compute interface pressure as a linear combination of these parameters. The pressures computed with this reduced model were in agreement with experimental pressure values measured on 66 patients' legs. Conclusion: This methodology helps to predict patient-specific interface pressure applied by compression bandages within a few minutes whereas it would take a few days for the numerical simulation. The results of this method show less bias than Laplace's Law, which is for now the only other method for interface pressure computation.
Published: 2018

33. Evolution of blood rheology and its relationship to pulmonary hemodynamic during the first days of exposure to moderate altitude

Author: Jorge Lopez Ayerbe, Patrick Mucci, Philippe Connes, Fabienne Durand, Antoine Raberin, Elie Nader, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université de Lille-Université du Littoral Côte d'Opale (ULCO), Laboratoire Européen Performance Santé Altitude (LEPSA), Université de Perpignan Via Domitia (UPVD), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), and Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - EA 7369 (URePSSS)
Subjects: Adult, Male, medicine.medical_specialty, Pulmonary Circulation, Physiology, Blood viscosity, Hemodynamics, 030204 cardiovascular system & hematology, Hematocrit, Acclimatization, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, [SCCO]Cognitive science, Young Adult, 0302 clinical medicine, Physiology (medical), Internal medicine, Hypoxic pulmonary vasoconstriction, medicine, Humans, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, business.industry, Altitude, Hematology, Hemoconcentration, Healthy Volunteers, Red blood cell, medicine.anatomical_structure, Vascular resistance, Cardiology, Cardiology and Cardiovascular Medicine, business, Rheology
Abstract: Blood rheology and hemodynamic parameters have never been explored together during acclimatization to altitude. This study aimed to investigate changes in blood rheology parameters and pulmonary hemodynamics during the first days of real moderate altitude exposure.Seventeen athletes were tested at sea-level, 20 hours after their arrival at 2,400 meters of altitude (H1) and five days later (H2). Blood was sampled to analyze red blood cell (RBC) aggregation, blood viscosity and hematocrit. Pulmonary arterial pressure (PAP), pulmonary capillary pressure (Pcap) and pulmonary vascular resistance (PVR) were assessed by echocardiography.We observed a rise in hematocrit, blood viscosity, RBC aggregation, PAP, Pcap and PVR between sea-level and H1. In H2, RBC aggregation, hematocrit, PAP, Pcap and PVR remained different compared to sea-level and no difference was observed between H1 and H2. Blood viscosity decreased in H2 and returned to sea-level values.Our results suggest that hemoconcentration occurring within the first hours of altitude exposure increased blood viscosity, which contributed to the changes in pulmonary hemodynamic. When blood viscosity decreased in H2, no change occurred in pulmonary hemodynamic parameters suggesting that hypoxic pulmonary vasoconstriction was still present. The elevated RBC aggregation observed after in H2 could participate in the increase of Pcap.
Published: 2019

34. Reptiles in Guadeloupe (French West Indies) are a reservoir of major human Salmonella enterica serovars

Author: Edith Malpote, Blandine Muanza, Séverine Ferdinand, Antoine Talarmin, Sylvaine Bastian, Cécile Loraux, Stéphanie Guyomard-Rabenirina, Sébastien Breurec, François-Xavier Weill, Pierre Legreneur, Vincent Richard, Simon Le Hello, Franck Berger, Unité Transmission, Réservoir et Diversité des Pathogènes [Pasteur Guadeloupe, France] (TReD-Path), Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS), Institut Pasteur [Paris] (IP), Laboratoire de Microbiologie clinique et environnementale [Pointe-à-Pitre, Guadeloupe, France], CHU Pointe-à-Pitre/Abymes [Guadeloupe], Centre d'épidémiologie et de santé publique des armées [Marseille] (CESPA), Service de Santé des Armées, Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale (SESSTIM - U1252 INSERM - Aix Marseille Univ - UMR 259 IRD), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service de Pédiatrie [Pointe-à-Pitre, Guadeloupe, France], Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Université des Antilles - UFR des sciences médicales Hyacinthe Bastaraud (UA UFR SM), Université des Antilles (UA), The Institut Pasteur was the funder of this study (https://www.pasteur.fr/fr)., We thank all the students and the technicians involved in this work at the Institut Pasteur of Guadeloupe, the University Hospital of Pointe-à-Pitre and the FNRC-ESS. We thank Nadia Babel for collecting data on livestock production., Bactéries pathogènes entériques (BPE), Institut Pasteur [Paris], Centre de Recherche et d'Innovation sur le Sport (EA647) (CRIS), Université de Lyon-Université de Lyon, Bodescot, Myriam, and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: 0301 basic medicine, Serotype, Bacterial Diseases, Salmonella, Salmonellosis, Subspecies, medicine.disease_cause, Pathology and Laboratory Medicine, Geographical locations, 0403 veterinary science, Medicine and Health Sciences, Guadeloupe, Panama, Multidisciplinary, Eukaryota, Salmonella enterica, Agriculture, 04 agricultural and veterinary sciences, 3. Good health, Bacterial Pathogens, Infectious Diseases, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Medical Microbiology, Salmonella Infections, Vertebrates, Medicine, Frogs, Livestock, Pathogens, Research Article, 040301 veterinary sciences, Science, 030106 microbiology, Zoology, Biology, Microbiology, Amphibians, 03 medical and health sciences, Enterobacteriaceae, medicine, Animals, Humans, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Microbial Pathogens, Feces, Disease Reservoirs, Caribbean, Bacteria, business.industry, Organisms, Biology and Life Sciences, Reptiles, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Carriage, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Case-Control Studies, Amniotes, North America, Iguanas, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, People and places, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, business
Abstract: International audience; The epidemiology of human Salmonella enterica infections in Guadeloupe (French West Indies) appears to be specific, with a higher prevalence of the subspecies enterica serovars Panama and Arechavaleta (Panama and Arechavaleta) than in other regions. A study was performed in Guadeloupe to identify the reservoir of Salmonella serovars by comparing their distribution in warm- and cold-blooded animals and in humans living in Guadeloupe and mainland France. Furthermore, a case-control study was conducted in 2012-2013 to identify the main epidemiologic risk factors for S. enterica infection among children under 15 years of age. Between June 2011 and December 2014, feces from 426 reptiles (322 anoles, 69 iguanas and 35 geckos) and 50 frogs distributed throughout Guadeloupe and nearby islands were investigated. The frequency of S. enterica carriage was 15.0% (n = 64) in reptiles but varied by species. The only significant risk factor for S. enterica infection was a more frequent presence of frogs in the houses of cases than in those of controls (P = 0.042); however, isolates were not collected. Panama and Arechavaleta were the two serovars most often recovered between 2005 and 2014 from humans living in Guadeloupe (24.5% (n = 174) and 11.5% (n = 82), respectively), which is in contrast to the low prevalence in mainland France (0.4%). Their presence at low frequencies in wild reptiles (4.6% (n = 3) and 3.1% (n = 2), respectively) and pigs (7.5% (n = 5) and 1.5% (n = 1), respectively) suggests a broad host range, and humans may be infected by indirect or direct contact with animals. These serovars are probably poorly adapted to humans and therefore cause more severe infections. The unusual subspecies houtenae serovar 43:z4,z32:- was a major subspecies in wild reptiles (24.6%, n = 16) and humans (9.4%, n = 67) but was not recovered from warm-blooded animals, suggesting that reptiles plays a key role in human infection.
Published: 2019

35. Spatial difference can occur between activated and damaged muscle areas following electrically‐induced isometric contractions

Author: David Bendahan, Maxime Guye, Julien Gondin, Augustin C. Ogier, Alexandre Fouré, Arnaud Le Troter, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Images et Modèles (I&M), Laboratoire d'Informatique et Systèmes (LIS), Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Male, 0301 basic medicine, Physiology, Vastus medialis, [SDV]Life Sciences [q-bio], Stimulation, Isometric exercise, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Isometric Contraction, medicine, Humans, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, Chemistry, Statistical mapping, Skeletal muscle, Muscle activation, Magnetic resonance imaging, Anatomy, Electric Stimulation, 030104 developmental biology, medicine.anatomical_structure, Female, 030217 neurology & neurosurgery, Spatial difference
Abstract: KEY POINTS T2 mapping combined to image registration and statistical parametric mapping analysis is a suitable methodology to accurately localize and compare the extent of both activated and damaged muscle areas. Activated muscle areas following electrically-induced isometric contractions are superficial, but damaged regions are muscle specific and can be related to the muscle morphology and/or the relative spatial position within a muscle group leading to potential intramuscular muscle shear strain. Tissues other than active skeletal muscle fibres can be altered during unaccustomed neuromuscular electrical stimulation-induced isometric contractions. ABSTRACT Skeletal muscle isometric contractions induced by neuromuscular electrical stimulation (NMES) exercise can generate damage within activated muscles. This study aimed at comparing the localization and the extent of NMES-activated muscle areas and induced damage regions using magnetic resonance imaging. Thirteen healthy subjects performed a single bout of NMES-induced isometric contractions known to induce a decrease in maximal voluntary isometric contraction (MVC) and increase in muscle volume and transverse relaxation time (T2 ). All the parameters were measured before, immediately after (POST), 7 days (D7), 14 days (D14) and 21 days (D21) after the NMES session. Spatial normalization of T2 maps were performed to compare the localization of muscle activation areas and damaged muscle regions from statistical mapping analyses. A significant decrease in MVC was found at POST (-26 ± 9%) and in delayed time at D7 (-20 ± 6%) and D14 (-12 ± 5%). Although muscle activation was statistically detected through T2 increase at POST in superficial parts of the two muscles located beneath the stimulation electrodes (i.e. vastus lateralis and vastus medialis), alterations quantified in a delayed time from increased T2 were mainly located in the deep muscle region of the vastus lateralis (+57 ± 24% of mean T2 ) and superficial area of the vastus medialis (+24 ± 16% of mean T2 ) at D7 and were still observed in whole muscle at D21. The discrepancy between activated and damaged areas in the vastus lateralis implies that tissues other than active skeletal muscle fibres were altered during unaccustomed NMES-induced isomeric contractions.
Published: 2019

36. Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model

Author: Elise Madeuf, Geoffroy Delcros, Emilie Vazeille, Vincent Pialoux, Nicolas Barnich, Pierre Sauvanet, Florie Maillard, Allison Teixeira, Pascal Sirvent, Nathalie Boisseau, Monique Etienne, Carole Groussard, Antoine Sourdrille, Yolanda F. Otero, Lydie Combaret, Laboratoire Mouvement Sport Santé (M2S), École normale supérieure - Cachan (ENS Cachan)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Brest (UBO)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques - Clermont Auvergne (AME2P), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Clermont Auvergne (UCA), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte - Clermont Auvergne (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Université Clermont Auvergne (UCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), USC-2018, Institut National de la Recherche Agronomique, Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne), Service d'Hépatologie Gastro-entérologie [CHU Clermont-Ferrand], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de Recherche en Nutrition Humaine Auvergne [CHU Clermont-Ferrand] (CRNH A), Direction de la recherche clinique et de l’innovation [CHU Clermont-Ferrand] (DRCI), Region AuvergneRhone-Alpes (PREVAMIC project), I-SITE project of the University of Clermont Auvergne (CAP 2025), INSERM (U1071), INRA (USC-2018), Association F. Aupetit (AFA), ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH Auvergne), and Pialoux, V.
Subjects: 0301 basic medicine, Male, Aging, MESH: Rats, Zucker, Adipose tissue, MESH: Physical Conditioning, Animal, MESH: Muscle, Skeletal / metabolism, High-Intensity Interval Training, medicine.disease_cause, Biochemistry, Interval training, Antioxidants, chemistry.chemical_compound, 0302 clinical medicine, Malondialdehyde, Medicine, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, MESH: Oxidative Stress, biology, lcsh:Cytology, Glutathione peroxidase, General Medicine, Oxidants, Lipoproteins, LDL, Adipose Tissue, Alimentation et Nutrition, High-intensity interval training, Research Article, medicine.medical_specialty, MESH: Rats, Article Subject, Superoxide dismutase, 03 medical and health sciences, Internal medicine, Physical Conditioning, Animal, Food and Nutrition, Animals, Obesity, lcsh:QH573-671, Xanthine oxidase, Muscle, Skeletal, Glutathione Peroxidase, business.industry, Superoxide Dismutase, MESH: Adipose Tissue / metabolism, NADPH Oxidases, 030229 sport sciences, Cell Biology, Rats, Rats, Zucker, Oxidative Stress, 030104 developmental biology, Endocrinology, chemistry, biology.protein, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Oxidative stress, Lipoprotein
Abstract: Background and Aim. Exercise is an effective strategy to reduce obesity-induced oxidative stress. The purpose of this study was to compare the effects of two training modalities (moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT)) on the pro/antioxidant status of different tissues in obese Zucker rats. Methods. Eight-week-old male Zucker rats (fa/fa, n=36) were subdivided in three groups: MICT, HIIT, and control (no exercise) groups. Trained animals ran on a treadmill (0° slope), 5 days/week for 10 weeks (MICT: 51 min at 12 m·min-1; HIIT: 6 sets of 3 min at 10 m·min-1 followed by 4 min at 18 m·min-1). Epididymal (visceral) and subcutaneous adipose tissue, gastrocnemius muscle, and plasma samples were collected to measure oxidative stress markers (advanced oxidation protein products (AOPP), oxidized low-density lipoprotein (oxLDL)), antioxidant system markers (ferric-reducing ability of plasma (FRAP), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities), and prooxidant enzymes (NADPH oxidase and xanthine oxidase (XO) activities, myeloperoxidase content). Results. Compared with the control, MICT increased GPx and catalase activities and the FRAP level in epididymal adipose tissue. HIIT increased the AOPP level in subcutaneous adipose tissue. In the muscle, HIIT increased both SOD and GPx activities and reduced the AOPP level, whereas MICT increased only SOD activity. Finally, plasma myeloperoxidase content was similarly decreased by both training modalities, whereas oxLDL was reduced only in the MICT group. Conclusion. Both HIIT and MICT improved the pro/antioxidant status. However, HIIT was more efficient than MICT in the skeletal muscle, whereas MICT was more efficient in epididymal adipose tissue. This suggests that oxidative stress responses to HIIT and MICT are tissue-specific. This could result in ROS generation via different pathways in these tissues. From a practical point of view, the two training modalities should be combined to obtain a global response in people with obesity.
Published: 2019

37. Risk factors for treatment failure in scabies: a cohort study

Author: Aussy, A., Houivet, E., Hébert, V., Colas‐Cailleux, H., Laaengh, N., Richard, C., Ouvry, M., Boulard, C., Leger, S., Litrowski, N., Bénichou, J., Joly, P., Béchu, Stéphane, Mion‐Mouton, N., Lafaurie, O., Barrel, A., Pulluard, M., Hamel, V., Dore, M.X., Duflo, E., Lardans‐Cassius, S., Rzeznik, J.C., Baricault, S., Unité de biostatistiques [CHU Rouen], Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU), Service de Dermatologie [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen], Physiopathologie, Autoimmunité, maladies Neuromusculaires et THErapies Régénératrices (PANTHER), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Photochimie moléculaire et macromoléculaire (PMM), Centre National de la Recherche Scientifique (CNRS)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie du CNRS (INC), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Alcatel-Thalès III-V lab (III-V Lab), THALES-ALCATEL, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Hôpital Charles Nicolle [Rouen], Normandie Université (NU)-Normandie Université (NU), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), THALES [France]-ALCATEL, and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Adult, Male, medicine.medical_specialty, Adolescent, [SDV]Life Sciences [q-bio], Administration, Oral, Beds, Dermatology, Administration, Cutaneous, Benzoates, Risk Assessment, Drug Administration Schedule, Cohort Studies, Scabies, Young Adult, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Ivermectin, Pharmacotherapy, Risk Factors, Internal medicine, Animals, Humans, Medicine, Treatment Failure, Child, Decontamination, ComputingMilieux_MISCELLANEOUS, Antiparasitic Agents, business.industry, Incidence (epidemiology), Infant, Odds ratio, medicine.disease, Confidence interval, 3. Good health, Child, Preschool, Ambulatory, [SDV.IMM]Life Sciences [q-bio]/Immunology, Drug Therapy, Combination, Female, France, Sarcoptes scabiei, business, Cohort study, medicine.drug
Abstract: Background Treatment failure, which occurs in about one-third of cases, is considered as a major factor in the increasing incidence of scabies in developed countries. Objectives To identify predictors of treatment failure of scabies in ambulatory populations. Methods This multicentre study compared the clinical characteristics and treatment modalities between a group of patients with scabies treated successfully and another group who were not cured 3 months after antiscabies treatment. Results In total 210 patients with a diagnosis of scabies were included, comprising 98 patients in the treatment success group and 112 in the treatment failure group. The main risk factors for treatment failure were (i) the use of only one type of treatment, topical benzyl benzoate (BB) or oral ivermectin, vs. the combination of both treatments [odds ratio (OR) 2·15, 95% confidence interval (CI) 1·22-3·77]; (ii) the use of a single intake (vs. two) of oral ivermectin (OR 10·2. 95% CI 4·49-23·2); (iii) intake of ivermectin during a meal vs. on an empty stomach (OR 4·31, 95% CI 1·89-9·84); (iv) absence of decontamination of furnishings (OR 8·72, 95% CI 3·50-21·8), in particular sofa and cushions (OR 5·90, 95% CI 2·34-14·9), mattresses (OR 4·16, 95% CI 1·35-12·8) or car seats (OR 6·57, 95% CI 3·27-13·2) and (v) absence of written documents explaining treatment modalities (OR 5·18, 95% CI 2·57-10·4). In multivariate analysis, treatment failure was mainly associated with (i) use of a single intake (vs. two) of ivermectin (OR 6·62, 95% CI 2·71-16·2); (ii) use of BB alone vs. two intakes of ivermectin (OR 3·51, 95% CI 1·55-7·95) and (iii) absence of decontamination of furniture with acaricides (OR 5·81, 95% CI 1·96-16·7). Conclusions Use of topical BB alone and a single intake (vs. two) of ivermectin are predictors of treatment failure.
Published: 2019

38. Visbrain: A Multi-Purpose GPU-Accelerated Open-Source Suite for Multimodal Brain Data Visualization

Author: Etienne Combrisson, Raphael Vallat, Christian O'Reilly, Mainak Jas, Annalisa Pascarella, Anne-lise Saive, Thomas Thiery, David Meunier, Dmitrii Altukhov, Tarek Lajnef, Perrine Ruby, Aymeric Guillot, Karim Jerbi, Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire scribens, École Polytechnique de Montréal (EPM), Laboratoire Traitement et Communication de l'Information (LTCI), Institut Mines-Télécom [Paris] (IMT)-Télécom Paris, Institut des Sciences Cognitives (ISC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Université de Montréal (UdeM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut Fédératif des Neurosciences de Lyon (IFNL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Centre Hospitalier le Vinatier [Bron]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Centre de recherche en neurosciences de Lyon - Lyon Neuroscience Research Center (CRNL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Computer science, brain, OpenGL, Biomedical Engineering, Neuroscience (miscellaneous), 050105 experimental psychology, lcsh:RC321-571, neuroscience, 03 medical and health sciences, [SCCO]Cognitive science, 0302 clinical medicine, Data visualization, Human–computer interaction, 0501 psychology and cognitive sciences, EEG, Graphics, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, visualization, open-source, ComputingMilieux_MISCELLANEOUS, Original Research, Graphical user interface, computer.programming_language, MEG, business.industry, Suite, [SCCO.NEUR]Cognitive science/Neuroscience, 05 social sciences, Modular design, Python (programming language), Computer Science Applications, Visualization, python, business, computer, 030217 neurology & neurosurgery
Abstract: We present Visbrain, a Python open-source package that offers a comprehensive visualization suite for neuroimaging and electrophysiological brain data. Visbrain consists of two levels of abstraction: (1) objects which represent highly configurable neuro-oriented visual primitives (3D brain, sources connectivity, etc.) and (2) graphical user interfaces for higher level interactions. The object level offers flexible and modular tools to produce and automate the production of figures using an approach similar to that of Matplotlib with subplots. The second level visually connects these objects by controlling properties and interactions through graphical interfaces. The current release of Visbrain (version 0.4.2) contains 14 different objects and three responsive graphical user interfaces, built with PyQt: Signal, for the inspection of time-series and spectral properties, Brain for any type of visualization involving a 3D brain and Sleep for polysomnographic data visualization and sleep analysis. Each module has been developed in tight collaboration with end-users, i.e., primarily neuroscientists and domain experts, who bring their experience to make Visbrain as transparent as possible to the recording modalities (e.g., intracranial EEG, scalp-EEG, MEG, anatomical and functional MRI). Visbrain is developed on top of VisPy, a Python package providing high-performance 2D and 3D visualization by leveraging the computational power of the graphics card. Visbrain is available on Github and comes with a documentation, examples, and datasets (http://visbrain.org).
Published: 2019

39. Fatigue centrale et sommeil dans les épreuves d’ultra-endurance

Author: Millet, Guillaume Y., Martin, Vincent, Temesi, John, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), University of Northumbria at Newcastle [United Kingdom], C. Herisson, S.Perrey, M. Julia, A. Dupeyron, J-C. Daviet, Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Martin, Vincent
Subjects: [SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], ComputingMilieux_MISCELLANEOUS
Abstract: National audience
Published: 2019

40. Pre-participation cardiovascular evaluation in Pacific Island athletes

Author: Richard Donnadieu, Corinne Braunstein, François Carré, Frédéric Roche, Jean-Paul Grangeon, Satu Viali, Jean-Claude Barthélémy, Jean-Marie Sabot, François-Xavier Raby, Jean-Claude Chatard, Gérard Papouin, Karl Isaaz, Christian Guivarch, Régis Dacquin, Florian Espinosa, Antoine Gerbay, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Direction des affaires sanitaires et sociales de Nouvelle-Calédonie, Centre hospitalier territorial Gaston-Bourret [Nouméa], Système Nerveux Autonome - Epidémiologie, Physiologie, Ingénierie, Santé (SNA-EPIS), Université Jean Monnet [Saint-Étienne] (UJM)-Centre Hospitalier Universitaire de Saint-Etienne, Laboratoire Traitement du Signal et de l'Image (LTSI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), This work was supported by a grant from Fond Pacific and the Ministère des Affaires Etrangères et du Développement international, AFD CTZ 1056 01 T and a grant from Philips industry., Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Système Nerveux Autonome - Epidémiologie, Physiologie, Ingénierie, Santé (SNA - EPIS), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Adult, Male, medicine.medical_specialty, Adolescent, Cardiomyopathy, Physical examination, 030204 cardiovascular system & hematology, Pacific Islands, Sudden cardiac death, Pre-participation cardiovascular evaluation, Electrocardiography, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, T wave, medicine, Humans, Melanesians, 030212 general & internal medicine, Family history, Child, Exercise, Physical Examination, medicine.diagnostic_test, biology, Athletes, business.industry, Training level, medicine.disease, biology.organism_classification, T wave inversion, Death, Sudden, Cardiac, Cardiovascular Diseases, Female, [SDV.IB]Life Sciences [q-bio]/Bioengineering, Patient Participation, Abnormality, Polynesians, Cardiology and Cardiovascular Medicine, business, Follow-Up Studies
Abstract: International audience; Background - Pre-participation cardiovascular evaluation (PPE) aims to detect cardiac disease with sudden cardiac death (SCD) risk. No study has focused on Pacific Island athletes. Methods - A total of 2281 Pacific Island athletes were studied with (i) a questionnaire on family, personal history and symptoms, (ii) a physical examination and (iii) a 12-lead ECG. Results - 85% presented a normal history and examination. A positive family history was 1.4-1.9 fold higher in Melanesians, Polynesians and Métis than in Caucasians, while a positive personal history, abnormal symptoms and abnormal examination was 1.3 fold higher in Melanesians and Métis than in others. Neither gender nor training level had a bearing on these results. Melanesians had higher T wave inversions (TWIs) in V2-V4 leads but had no CV abnormalities. Lateral or infero-lateral TWIs were found in 6 male and in 5 highly trained athletes and cardiomyopathies were diagnosed in 3/6 athletes. Overall, 3.9% athletes were found to have a CV abnormality and 0.8% had a risk of SCD. Polynesians and males were more at risk than the others while the level of training made no difference. In athletes at risk of SCD, the main detected CV diseases were cardiomyopathies, Wolff-Parkinson-White (WPW) and severe valve lesions of rheumatoid origin. Conclusions - PPE revealed that 3.9% presented CV abnormalities. A risk of SCD was found in 0.8% with cardiomyopathies, WPW, and severe valve lesions of rheumatoid origin. Melanesians, Polynesians and male of high level of training were more at risk than others.
Published: 2019

41. Ischaemia-induced muscle metabolic abnormalities are poorly alleviated by endurance training in a mouse model of sickle cell disease

Author: David Bendahan, Vincent Pialoux, Benjamin Chatel, Christophe Vilmen, Monique Bernard, Laurent Messonnier, Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Exercise Physiology, University of Savoie, Chambery, and University Jean Monnet of Saint-Etienne, Saint-Etienne, Laboratory of Exercise Physiology, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire de Physiologie de l'Exercice (LPE), Université Jean Monnet - Saint-Étienne (UJM), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, Phosphocreatine, [SDV]Life Sciences [q-bio], Ischemia, Anemia, Sickle Cell, 030204 cardiovascular system & hematology, medicine.disease_cause, Mice, 03 medical and health sciences, chemistry.chemical_compound, Basal (phylogenetics), Adenosine Triphosphate, 0302 clinical medicine, Endurance training, Physical Conditioning, Animal, hemic and lymphatic diseases, Internal medicine, medicine, Animals, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, Acidosis, business.industry, Skeletal muscle, General Medicine, medicine.disease, Disease Models, Animal, Endurance Training, Oxidative Stress, medicine.anatomical_structure, Endocrinology, chemistry, Uric acid, medicine.symptom, business, Biomarkers, 030217 neurology & neurosurgery, Oxidative stress
Abstract: What is the central question of this study? The aim of this study was to evaluate the potential beneficial effects of endurance training during an ischaemia-reperfusion protocol in a mouse model of sickle cell disease (SCD). What is the main finding and its importance? Endurance training did not reverse the metabolic defects induced by a simulated vaso-occlusive crisis in SCD mice, with regard to intramuscular acidosis, mitochondrial dysfunction or anatomical properties. Our results suggest that endurance training would reduce the number of vaso-occlusive crises rather than the complications related to vaso-occlusive crises.The aim of this study was to investigate whether endurance training could limit the abnormalities described in a mouse model of sickle cell disease (SCD) in response to an ischaemia-reperfusion (I/R) protocol. Ten sedentary (HbSS-SED) and nine endurance-trained (HbSS-END) SCD mice were submitted to a standardized protocol of I/R of the leg, during which ATP, phosphocreatine and inorganic phosphate concentrations and intramuscular pH were measured using magnetic resonance spectroscopy. Forty-eight hours later, skeletal muscles were harvested. Oxidative stress markers were then measured. Although the time course of protons accumulation was slightly different between trained and sedentary mice (P 0.05), the extent of acidosis was similar at the end of the ischaemic period. The initial rate of phosphocreatine resynthesis measured at blood flow restoration, illustrating mitochondrial function, was not altered in trained mice compared with sedentary mice. Although several oxidative stress markers were not different between groups (P 0.05), the I/R-related increase of uric acid concentration observed in sedentary SCD mice (P 0.05) was not present in the trained group. The spleen weight, generally used as a marker of the severity of the disease, was not different between groups (P 0.05). In conclusion, endurance training did not limit the metabolic consequences of an I/R protocol in skeletal muscle of SCD mice, suggesting that the reduction in the severity of the disease previously demonstrated in the basal state would be attributable to a reduction of the occurrence of vaso-occlusive crises rather than a decrease of the deleterious effects of vaso-occlusive crises.
Published: 2019

42. Automatic depletion with Spectra Optia allows a safe 16% reduction of red blood cell pack consumption in exchanged sickle cell anemia patients

Author: Kamila Kebaili, Olivier Hequet, Alexandra Gauthier, Yves Bertrand, Solène Poutrel, A. Guironnet‐Paquet, Y. Chelghoum, M. Raba, Arnaud Hot, Giovanna Cannas, Jean-François Nicolas, Philippe Connes, Daniela Revesz, Céline Renoux, Marc Vocanson, Fabrice Cognasse, Philippe Joly, Engineering Service, Edouard Herriot Hospital, Service de Médecine Interne [Hôpital Edouard Herriot - HCL], Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Excellence : Biogenèse et pathologies du globule rouge (Labex Gr-Ex), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS), Service d'hématologie : Immuno-Hématologie pédiatrique et transplantation de moelle osseuse, Hôpital Debrousse, Hospices Civils de Lyon (HCL), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire national des champs magnétiques intenses - Grenoble [2016-2019] (LNCMI-G [2016-2019]), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Service de Médecine Interne, Laboratoire Aimé Cotton (LAC), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire national des champs magnétiques intenses - Grenoble (LNCMI), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UPS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Institut d'hématologie et oncologie pédiatriques, 69008 Lyon, France, parent, Immunologie de l'allergie cutanée et vaccination – Immunology of skin allergy and vaccination, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Institut d'hématologie et d'oncologie pédiatrique [CHU - HCL] (IHOPe), Groupe Immunité des Muqueuses et Agents Pathogènes (GIMAP), Université Jean Monnet [Saint-Étienne] (UJM), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Université Jean Monnet - Saint-Étienne (UJM), and Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3)
Subjects: Adult, Male, Erythrocytes, Adolescent, Anemia, [SDV]Life Sciences [q-bio], Immunology, Blood volume, Anemia, Sickle Cell, 030204 cardiovascular system & hematology, Hematocrit, 03 medical and health sciences, Young Adult, [SCCO]Cognitive science, 0302 clinical medicine, Heart rate, medicine, Immunology and Allergy, Humans, Prospective Studies, Adverse effect, Child, ComputingMilieux_MISCELLANEOUS, medicine.diagnostic_test, business.industry, Hematology, medicine.disease, Sickle cell anemia, 3. Good health, Red blood cell, medicine.anatomical_structure, Blood pressure, Anesthesia, Female, business, Erythrocyte Transfusion, 030215 immunology
Abstract: Background Chronic red blood cell exchanges (RBCXs) are frequently used to prevent complications in patients with sickle cell anemia, but the scarcity of matched red blood cell packs (RBCPs) is a serious concern. The main goal of this study was to compare the number of RBCPs used during RBCXs between the Spectra Optia (SO) device (with the automatic depletion step) and the former Cobe Spectra (CSP) device. Study design and methods The performances and safety of 300 SO sessions using the automatic depletion step (SO/DE) in 50 patients with sickle cell anemia under a chronic transfusion program over a 1-year period were prospectively analyzed. The numbers of RBCPs saved using this protocol compared to the SO device without depletion and to the CSP device were determined. Results The SO/DE protocol appeared to be safe, as only 5% and 17% of the sessions were characterized by a significant decrease in blood pressure and increase in heart rate (grade 2 adverse events), respectively. Postapheresis hematocrit and fraction of cells remaining reached expected values. The SO/DE protocol required 16% fewer RBCPs compared to SO without depletion, allowing a mean saving of 12 RBCPs per patient and per year and 13% fewer compared to CSP device. Interestingly, the saving was more important for patients with high total blood volume and/or high preapheresis hematocrit. Conclusion The SO/DE protocol is an efficient, safe and cost-effective procedure for patients with sickle cell anemia under a chronic transfusion program.
Published: 2019

43. Muscle loss associated changes of oxylipin signatures during biological aging: an exploratory study from the PROOF cohort

Author: Alice Decourt, Cécile Coudy-Gandilhon, Annika I. Ostermann, Thade Konrad, Cécile Gladine, Daniel Béchet, Nils Helge Schebb, Céline Dalle, Léonard Féasson, Frédéric Roche, André Mazur, Jean-Claude Barthélémy, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Faculty of Mathematics and Natural Sciences, University of Wuppertal, University of Veterinary Medicine Hanover, Service de Physiologie Clinique et de l'Exercice, Faculté de Médecine Jacques Lisfranc, PRES Université de Lyon, Université Jean Monnet (Saint-Etienne), Université de Lyon, Service de Neurologie, Centre Hospitalier du Pays d'Aix, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), German Resaerch Foundation SCHE 1801Fondation Caisse d'Epargne Rhone-Alpes (CERA) 30French government IDEX-ISITE initiative 16-IDEX-0001 CAP 20-25French Ministry of Health, ProdInra, Archive Ouverte, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Institute for Food Toxicology, University of Veterinary Medicine, Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, service de chirurgie cardiaque, Centre Hospitalier Universitaire Gabriel Montpied, Service de Physiologie Clinique et de l'Exercice, CHU Nord, Faculté de Médecine Jacques Lisfranc, Unité de Myologie, Centre Référent Maladies Neuromusculaires Rares Rhône-Alpes, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Unité de Myologie, Centre Hospitalier Universitaire de Saint-Etienne, Service de Physiologie Clinique et de l'Exercice [CHU de Saint-Etienne], Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Université Jean Monnet - Saint-Étienne (UJM), Université Jean Monnet - Saint-Étienne (UJM), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Université Catholique de Louvain (UCLouvain). BEL., CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Institut Multidisciplinaire de Biochimie des Lipides (IMBL). FRA., Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), and Centre Hospitalier Universitaire de Saint-Etienne ( CHU de Saint-Etienne )
Subjects: 0301 basic medicine, Male, Aging, Sarcopenia, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, Lipid mediators, Inflammation, medicine.disease_cause, Bioinformatics, analyse métabolomique, 03 medical and health sciences, 0302 clinical medicine, Metabolomics, Absorptiometry, Photon, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, Humans, Longitudinal Studies, Oxylipins, Prospective Studies, perte de muscle, Aged, 80 and over, business.industry, Lipid signaling, vieillissement, Oxylipin, medicine.disease, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, 030104 developmental biology, oxylipine, Ageing, ageing, Cohort, Eicosanoids, Female, Geriatrics and Gerontology, medicine.symptom, business, étude de cohorte, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, Oxidative stress, Biomarkers
Abstract: L’Unité de Nutrition Humaine (UNH - Clermont-Ferrand) organise cette année avec l’aide du laboratoire CarMeN (Lyon) la 18ème éditionL’Unité de Nutrition Humaine (UNH - Clermont-Ferrand) organise cette année avec l’aide du laboratoire CarMeN (Lyon) la 18ème édition; Characterizations of the multiple mechanisms determining biological aging are required tobetter understand the etiology and identify early biomarkers of sarcopenia. Oxylipins are a largefamily of signaling lipids involved in the regulation of various biological processes that becomedysregulated during aging.To investigate whether comprehensive oxylipin profiling could provide an integrated and finecharacterisation of the early phases of sarcopenia, we performed a quantitative targetedmetabolomics of oxylipins in plasma of 81-year old subjects from the PROOF cohort withdecreased (n=12), stable (n=16) or increased appendicular muscle mass (n=14).Multivariate and univariate analyses identified significant and concordant changes of oxylipinprofiles according to the muscle status. Of note, 90% of the most discriminant oxylipins werederived from EPA and DHA and were increased in the sarcopenic subjects. The oxylipinssignatures of sarcopenic subjects revealed subtle activation of inflammatory resolutionpathways, coagulation processes and oxidative stress and the inhibition of angiogenesis. Heatmaps highlighted relationships between oxylipins and the cardiometabolic health parameterswhich were mainly lost in sarcopenic subjects.This exploratory study supports that targeted metabolomics of oxylipins could provide relevantand subtle characterization of early disturbances associated with muscle-loss during aging.
Published: 2019

44. Feasibility and health benefits of an individualized physical activity intervention in women with metastatic breast cancer: results of the ABLE single-arm Trial study

Author: Delrieu, Lidia, Pialoux, Vincent, Pérol, Olivia, Morelle, Magali, Martin, Agnès, Friedenreich, Christine, Febvey-Combes, Olivia, Pérol, David, Belladame, Elodie, Clémençon, Michel, Roitmann, Eva, Dufresne, Armelle, Bachelot, Thomas, Heudel, Pierre-Étienne, Touillaud, Marina, Trédan, Olivier, Fervers, Béatrice, Centre Léon Bérard [Lyon], Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Groupe d'analyse et de théorie économique (GATE Lyon Saint-Étienne), École normale supérieure - Lyon (ENS Lyon)-Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Centre National de la Recherche Scientifique (CNRS), Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Institut National de la Recherche Agronomique (INRA)-Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Division of Population Health and Information, Alberta Cancer Board, Centre d’études des transformations des activités physiques et sportives (CETAPS), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut de Recherche Interdisciplinaire Homme et Société (IRIHS), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Equipe 11, Service d'oncologie Médicale, Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Hôpital Jean Minjoz-Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon)-Hôpital Jean Minjoz-Centre de Recherche en Cancérologie de Lyon (CRCL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre Léon Bérard [Lyon]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Oncogénèse et progression tumorale, Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Réseau National Alimentation Cancer Recherche (réseau NACRe), Institut National de la Recherche Agronomique (INRA), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Groupe d'Analyse et de Théorie Economique Lyon - Saint-Etienne (GATE Lyon Saint-Étienne), École normale supérieure de Lyon (ENS de Lyon)-Université Lumière - Lyon 2 (UL2)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université Lumière - Lyon 2 (UL2)-École normale supérieure - Lyon (ENS Lyon), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Service d'Oncologie Médicale [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Subjects: [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, [SHS.ECO]Humanities and Social Sciences/Economics and Finance, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

45. Persistent low body weight in humans is associated with higher mitochondrial activity in white adipose tissue

Author: Léonard Féasson, Alice Matone, John Corthésy, Nele Gheldof, Loïc Dayon, Jérôme Carayol, Francois Casteillo, Virginie Alexandre, Christophe Montaurier, Aline Charpagne, Carles Cantó, Kaori Minehira, Natacha Germain, Radu Palaghiu, Yiin Ling, Bogdan Galusca, Antonio Núñez Galindo, Patrick Descombes, Michel Peoc'h, Jörg Hager, Irene Vassallo, Sylviane Metairon, Frederic Raymond, Ornella Cominetti, Yves Boirie, Bruno Estour, Endocrinologie, diabète et maladies métaboliques, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), EA 7423, Centre Hospitalier Universitaire de Saint Etienne, Université Jean Monnet (Saint-Etienne), Nestlé Research Center, Metabolic Health, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Centre for Computational and Systems Biology (COSBI), Quartzbio, Proteomics, Center for Applied Medical Research (CIMA), Genomics, Biotec, Dresden, Chirurgie, CHU Metz-Hôpital Bon Secours, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Nutrition Clinique, CHU Clermont-Ferrand, Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), Université Jean Monnet - Saint-Étienne (UJM), Nestlé Research Center | Centre de recherche Nestlé [Lausanne], Nestlé S.A., Nestlé S.A.-Nestlé S.A., Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Pathologie, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-AP-HP Hôpital Raymond Poincaré [Garches], and Centre Hospitalier Universitaire de Clermont-Ferrand
Subjects: 0301 basic medicine, Adult, Male, medicine.medical_specialty, Time Factors, Adipose Tissue, White, Adipocytes, White, Medicine (miscellaneous), Adipose tissue, 030209 endocrinology & metabolism, White adipose tissue, 03 medical and health sciences, Young Adult, 0302 clinical medicine, Overnutrition, proteomics, transcriptome analysis, white adipose tissue, Thinness, Internal medicine, Weight management, medicine, Humans, Beta oxidation, 2. Zero hunger, Nutrition and Dietetics, constitutional thinness, business.industry, Gene Expression Profiling, medicine.disease, Lipids, Mitochondria, Original Research Communications, 030104 developmental biology, Endocrinology, Case-Control Studies, Female, medicine.symptom, business, futile lipid cycling, Energy Intake, Transcriptome, Body mass index, Weight gain, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, respiration, Dieting
Abstract: Epub ahead of print; BACKGROUND:Constitutional thinness (CT) is a state of low but stable body weight (BMI ≤18 kg/m2). CT subjects have normal-range hormonal profiles and food intake but exhibit resistance to weight gain despite living in the modern world's obesogenic environment.OBJECTIVE:The goal of this study is to identify molecular mechanisms underlying this protective phenotype against weight gain.METHODS:We conducted a clinical overfeeding study on 30 CT subjects and 30 controls (BMI 20-25 kg/m2) matched for age and sex. We performed clinical and integrative molecular and transcriptomic analyses on white adipose and muscle tissues.RESULTS:Our results demonstrate that adipocytes were markedly smaller in CT individuals (mean ± SEM: 2174 ± 142 μm 2) compared with controls (3586 ± 216 μm2) (P < 0.01). The mitochondrial respiratory capacity was higher in CT adipose tissue, particularly at the level of complex II of the electron transport chain (2.2-fold increase; P < 0.01). This higher activity was paralleled by an increase in mitochondrial number (CT compared with control: 784 ± 27 compared with 675 ± 30 mitochondrial DNA molecules per cell; P < 0.05). No evidence for uncoupled respiration or "browning" of the white adipose tissue was found. In accordance with the mitochondrial differences, CT subjects had a distinct adipose transcriptomic profile [62 differentially expressed genes (false discovery rate of 0.1 and log fold change >0.75)], with many differentially expressed genes associating with positive metabolic outcomes. Pathway analyses revealed an increase in fatty acid oxidation ( P = 3 × 10-04) but also triglyceride biosynthesis (P = 3.6 × 10-04). No differential response to the overfeeding was observed in the 2 groups.CONCLUSIONS:The distinct molecular signature of the adipose tissue in CT individuals suggests the presence of augm ented futile lipid cycling, rather than mitochondrial uncoupling, as a way to increase energy expenditure in CT individuals. We propose that increased mitochondrial function in adipose tissue is an important mediator in sustaining the low body weight in CT individuals. This knowledge could ultimately allow more targeted approaches for weight management treatment strategies. This trial was registered at clinicaltrials.gov as NCT02004821.
Published: 2019

46. How Sickle Cell Disease Impairs Skeletal Muscle Function

Author: Merlet, Angèle, Chatel, Benjamin, Hourdé, Christophe, Ravelojaona, Marion, Bendahan, David, Feasson, Léonard, Messonnier, Laurent, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie de l'Exercice (LPE), Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratory of Exercise Physiology, University of Savoie, Chambery, and University Jean Monnet of Saint-Etienne, Saint-Etienne, and Laboratory of Exercise Physiology
Subjects: [SDV]Life Sciences [q-bio], [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2019

47. In-silico pre-clinical trials are made possible by a new simple and comprehensive lumbar belt mechanical model based on the Law of Laplace including support deformation and adhesion effects

Author: Reynald Convert, Paul Calmels, Jérôme Molimard, Woo Suck Han, Rébecca Bonnaire, École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT), Institut Clément Ader (ICA), Institut Supérieur de l'Aéronautique et de l'Espace (ISAE-SUPAERO)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-IMT École nationale supérieure des Mines d'Albi-Carmaux (IMT Mines Albi), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Thuasne, Université Jean Monnet - Saint-Étienne (UJM), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IMT École nationale supérieure des Mines d'Albi-Carmaux (IMT Mines Albi), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut Supérieur de l'Aéronautique et de l'Espace (ISAE-SUPAERO), Université Jean Monnet [Saint-Étienne] (UJM), and Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, Bending, Physiology, 02 engineering and technology, Pathology and Laboratory Medicine, Stiffness, Abdomen, Medicine and Health Sciences, Musculoskeletal System, Body mass index, Lumbar Vertebrae, Multidisciplinary, Deformation (mechanics), Physics, Classical Mechanics, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], Structural engineering, Middle Aged, 021001 nanoscience & nanotechnology, Deformation, 3. Good health, Physiological Parameters, Physical Sciences, Lordosis, Bending moment, Medicine, Female, Anatomy, medicine.symptom, 0210 nano-technology, Geology, Research Article, Adult, Body shape, Science, Materials Science, Material Properties, 0206 medical engineering, Pain, Geometry, Curvature, Models, Biological, Signs and Symptoms, Lumbar, Diagnostic Medicine, parasitic diseases, medicine, Humans, Mechanical Properties, Lower back pain, Damage Mechanics, business.industry, Body Weight, Lumbosacral Region, technology, industry, and agriculture, Biology and Life Sciences, equipment and supplies, 020601 biomedical engineering, Trunk, Spine, business, Low Back Pain, human activities, Mathematics
Abstract: International audience; Lower back pain is a major public health problem. Despite claims that lumbar belts change spinal posture due to applied pressure on the trunk, no mechanical model has yet been published to prove this treatment. This paper describes a first model for belt design, based on the one hand on the mechanical properties of the fabrics and the belt geometry, and on the other hand on the trunk geometrical and mechanical description. The model provides the estimation of the pressure applied to the trunk, and a unique indicator of the belt mechanical efficiency is proposed: pressure is integrated into a bending moment characterizing the belt delordosing action on the spine. A first in-silico clinical study of belt efficiency for 15 patients with 2 different belts was conducted. Results are very dependent on the body shape: in the case of high BMI patients, the belt effect is significantly decreased, and can be even inverted, increasing the lordosis. The belt stiffness proportionally increases the pressure applied to the trunk, but the influence of the design itself on the bending moment is clearly outlined. Moreover, the belt/trunk interaction, modeled as sticking contact and the specific way patients lock their belts, dramatically modifies the belt action. Finally, even if further developments and tests are still necessary, the model presented in this paper seems suitable for in-silico pre-clinical trials on real body shapes at a design stage.
Published: 2019

48. Reduced fibre size, capillary supply and mitochondrial activity in constitutional thinness' skeletal muscle

Author: Yiin Ling, Emmanuelle Meugnier, Julien Verney, Bruno Estour, Pascal Edouard, Hubert Vidal, Léonard Féasson, Bogdan Galusca, M. Ravelojaona, Natacha Germain, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques - Clermont Auvergne (AME2P), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Clermont Auvergne (UCA), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Jean Le Rond d'Alembert (DALEMBERT), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Jean Monnet (Saint-Etienne), Centre Référent Maladies Neuromusculaires Rares Rhône-Alpes, Unité de Myologie, Centre Hospitalier Universitaire de Saint-Etienne (CHU de Saint-Etienne), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Institut Jean le Rond d'Alembert (DALEMBERT), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Service de physiologie clinique, unité de myologie, CHU Saint-Etienne, French Health Ministry regional grant 0908019, GlaxoSmithKline France Funds, Université Jean Monnet - Saint-Étienne (UJM), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)
Subjects: 0301 basic medicine, medicine.medical_specialty, Physiology, [SDV]Life Sciences [q-bio], Muscle Fibers, Skeletal, Respiratory chain, 030209 endocrinology & metabolism, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, skeletal muscle fibres, Internal medicine, mitochondrial respiration, Myokine, medicine, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Animals, Humans, Myocyte, Cytochrome c oxidase, Muscle, Skeletal, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, biology, constitutional thinness, business.industry, Body Weight, Skeletal muscle, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, energy balance, Mitochondria, Muscle, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Case-Control Studies, Basal metabolic rate, Body Composition, biology.protein, Myokines, Female, medicine.symptom, business, Weight gain, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: International audience; Aim Constitutional thinness (CT) is a rare condition of natural low body weight, with no psychological issues, no marker of undernutrition and a resistance to weight gain. This study evaluated the skeletal muscle phenotype of CT women by comparison with a normal BMI control group. MethodsResultsTen CT women (BMI
Published: 2018

49. The genetic basis of asymptomatic codon 8 frame-shift (HBB:c25_26delAA) β0-thalassaemia homozygotes

Author: Sule Unal, Bernard G. Forget, Nejat Akar, Martin H. Steinberg, A. Nazli Basak, Fatma Gumruk, Catherine Badens, Patrick G. Gallagher, Leonor Osorio, Serge Pissard, Nasir A. S. Al-Allawi, Roger Théberge, Andrew D. Campbell, John J. Farrell, Zhihua Jiang, Suchada Riolueang, Philippe Joly, Hong-Yuan Luo, Shengwen Huang, Katherine A. Benson, Vip Viprakasit, Lance Davis, David H.K. Chui, Departments of Medicine, Pathology and Laboratory Medicine, Boston University School of Medicine (BUSM), Boston University [Boston] (BU)-Boston University [Boston] (BU), Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Genetique, UPEC (Universite' Paris Est Creteil), Laboratoire de Genetique, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), University of Michigan [Ann Arbor], University of Michigan System, Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)
Subjects: Male, 0301 basic medicine, congenital, hereditary, and neonatal diseases and abnormalities, Genes, myb, [SDV]Life Sciences [q-bio], Dizygotic twin, Quantitative Trait Loci, Single-nucleotide polymorphism, Biology, Polymorphism, Single Nucleotide, HBG2, Frameshift mutation, Loss of heterozygosity, Young Adult, 03 medical and health sciences, Polymorphism (computer science), hemic and lymphatic diseases, Fetal hemoglobin, Diseases in Twins, Twins, Dizygotic, medicine, Humans, Frameshift Mutation, Fetal Hemoglobin, ComputingMilieux_MISCELLANEOUS, Genetics, Homozygote, beta-Thalassemia, Nuclear Proteins, Beta thalassemia, Hematology, Middle Aged, medicine.disease, Molecular biology, Repressor Proteins, 030104 developmental biology, Female, Carrier Proteins
Abstract: Two 21-year old dizygotic twin men of Iraqi descent were homozygous for HBB codon 8, deletion of two nucleotides (-AA) frame-shift β(0) -thalassaemia mutation (FSC8; HBB:c25_26delAA). Both were clinically well, had splenomegaly, and were never transfused. They had mild microcytic anaemia (Hb 120-130 g/l) and 98% of their haemoglobin was fetal haemoglobin (HbF). Both were carriers of Hph α-thalassaemia mutation. On the three major HbF quantitative trait loci (QTL), the twins were homozygous for G>A HBG2 Xmn1 site at single nucleotide polymorphism (SNP) rs7482144, homozygous for 3-bp deletion HBS1L-MYB intergenic polymorphism (HMIP) at rs66650371, and heterozygous for the A>C BCL11A intron 2 polymorphism at rs766432. These findings were compared with those found in 22 other FSC8 homozygote patients: four presented with thalassaemia intermedia phenotype, and 18 were transfusion dependent. The inheritance of homozygosity for HMIP 3-bp deletion at rs66650371 and heterozygosity for Hph α-thalassaemia mutation was found in the twins and not found in any of the other 22 patients. Further studies are needed to uncover likely additional genetic variants that could contribute to the exceptionally high HbF levels and mild phenotype in these twins.
Published: 2016

50. Inflammatory Response 24 h Post-Exercise in Youth with Juvenile Idiopathic Arthritis

Author: Christophe Hourdé, Stéphane Echaubard, Emmanuelle Rochette, Pascale Duché, Etienne Merlin, Bruno Peraira, Bertrand Evrard, CHU Clermont-Ferrand, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Impact de l'Activité Physique sur la Santé (IAPS), Université de Toulon (UTLN), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])
Subjects: Male, Time Factors, [SDV]Life Sciences [q-bio], Arthritis, Pituitary-Adrenal System, Systemic inflammation, Gastroenterology, [SHS]Humanities and Social Sciences, 0302 clinical medicine, Cytokine Receptor gp130, Medicine, Orthopedics and Sports Medicine, Child, ComputingMilieux_MISCELLANEOUS, Pain Measurement, biology, Venous blood, Disease Progression, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, medicine.symptom, medicine.medical_specialty, Hypothalamo-Hypophyseal System, Adolescent, Physical Therapy, Sports Therapy and Rehabilitation, Proinflammatory cytokine, 03 medical and health sciences, Adrenocorticotropic Hormone, Internal medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Juvenile, Humans, Exercise physiology, Interleukin 6, Exercise, 030203 arthritis & rheumatology, [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics, business.industry, Interleukin-6, 030229 sport sciences, Dehydroepiandrosterone, medicine.disease, Receptors, Interleukin-6, Arthritis, Juvenile, biology.protein, Calprotectin, business, Leukocyte L1 Antigen Complex, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: The aim of this study was to measure the impact, at 24 h post-exercise, of a single exercise bout on plasma inflammatory markers such as calprotectin, IL-6, sIL-6 R, sgp130 and the hypothalamic-pituitary-adrenal (HPA) axis in children with juvenile idiopathic arthritis (JIA).Twelve children with JIA attended the laboratory on three consecutive days (control day, exercise day and 24 h post-exercise), including a 20-min exercise bout on a cycle-ergometer at 70% of max. HR at 8:30 a.m. on day 2. Plasma concentrations of calprotectin, IL-6, sIL-6 R, sgp130, cortisol, ACTH and DHEA were measured on venous blood samples taken every day.at rest and at 8:30, 8:50, 9:30, 10:30 a.m. and 12:00, 3:00, 5:30 p.m.A single exercise bout increased plasma calprotectin 1.7-fold (pPhysical activity in children with JIA results in a slight transient systemic inflammation but seems to be followed by counter-regulation at 24 h post-exercise with a decrease in proinflammatory markers.
Published: 2018

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