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Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Singlebla(CTX-M-1)/IncI1/ST3 Plasmid Among Humans and Wild Animals
- Source :
- Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2020-07, Vol. 11, P. 1524 (11p.), Frontiers in Microbiology, Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.01524⟩, Frontiers in Microbiology, Frontiers Media, 2020, 11, ⟨10.3389/fmicb.2020.01524⟩, Frontiers in Microbiology, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media Sa, 2020.
-
Abstract
- Limited data are available on the contribution of wildlife to the spread of antibacterial resistance. We determined the prevalence of resistance to antibiotics in Escherichia coli isolates collected from wild animals in 2013 and 2014 and the genetic basis for resistance to third-generation cephalosporin in Guadeloupe. We recovered 52 antibiotic-resistant (AR) E. coli strains from 48 of the 884 (5.4%) wild animals tested (46 iguanas, 181 birds, 289 anoles, and 368 rodents at 163 sampling sites). Rodents had higher rates of carriage (n = 38, 10.3%) than reptiles and birds (2.4% and 1.1%, respectively, p < 0.001). A significant association (p < 0.001) was found between the degree of anthropization and the frequency of AR E. coli carriage for all species. The carriage rate of ciprofloxacin- and cefotaxime-resistant isolates was 0.7% (6/884) and 1.5% (13/884), respectively. Most (65.4%) AR E. coli were multi-drug resistant, and the prevalence of extended-spectrum beta-lactamase (ESBL)-producing E. coli was low (n = 7, 0.8%) in all species. Eight ESBL-producing E. coli were recovered, two genetically unrelated isolates being found in one bird. These isolates and 20 human invasive ESBL E. coli isolates collected in Guadeloupe during the same period were investigated by whole genome sequencing. blaCTX–M–1 was the only ESBL gene shared by three animal classes (humans, n = 2; birds, n = 2; rodents, n = 2). The blaCTX–M–1 gene and most of the antimicrobial resistance genes were present in a large conjugative IncI1 plasmid that was highly similar (>99% nucleotide identity) to ESBL-carrying plasmids found in several countries in Europe and in Australia. Although the prevalence of ESBL-producing E. coli isolates was very low in wild animals, it is of concern that the well-conserved IncI1 plasmid-carrying blaCTX–M–1 is widespread and occurs in various E. coli strains from animals and humans.
- Subjects :
- Microbiology (medical)
Veterinary medicine
medicine.drug_class
[SDV]Life Sciences [q-bio]
Antibiotics
Cephalosporin
Wildlife
lcsh:QR1-502
extended-spectrum beta-lactamase
Biology
medicine.disease_cause
Microbiology
lcsh:Microbiology
03 medical and health sciences
Antibiotic resistance
Plasmid
plasmid
wild animals
medicine
Escherichia coli
antimicrobial resistance
030304 developmental biology
West indies
Original Research
0303 health sciences
030306 microbiology
3. Good health
[SDV] Life Sciences [q-bio]
Carriage
Subjects
Details
- Language :
- English
- ISSN :
- 1664302X
- Database :
- OpenAIRE
- Journal :
- Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2020-07, Vol. 11, P. 1524 (11p.), Frontiers in Microbiology, Frontiers in Microbiology, 2020, 11, ⟨10.3389/fmicb.2020.01524⟩, Frontiers in Microbiology, Frontiers Media, 2020, 11, ⟨10.3389/fmicb.2020.01524⟩, Frontiers in Microbiology, Vol 11 (2020)
- Accession number :
- edsair.doi.dedup.....2a2ce9561f93a73b000d7cb97524c64a