323 results on '"Qing Pang"'
Search Results
2. Platelet count as a predictor of vascular invasion and extrahepatic metastasis in hepatocellular carcinoma: A systematic review and meta-analysis
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Qing Pang, Xuankun Gong, Hongtao Pan, Yong Wang, Xiaosi Hu, Huichun Liu, and Hao Jin
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Hepatocellular carcinoma ,Platelet count ,Vascular invasion ,Extrahepatic metastasis ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Vascular invasion (VI) indicates highly invasive tumor biological behavior and is a major determining factor of poor survival and high risk of metastasis in hepatocellular carcinoma (HCC). Epidemiological evidence of the association between pretherapeutic platelet count (PLT) and the risk of VI and extrahepatic metastasis in HCC remains controversial. Methods: A systematic retrieval was executed in databases of PubMed, Embase, and Web of Science until Dec 2022. Effect size and 95% confidence interval (CI) were extracted or estimated to synthetically investigate the effects of pretherapeutic PLT on VI and extrahepatic metastasis. Meta-analyses were performed by using a random or a fixed effects model. Results: Finally, the current meta-analysis included 15 studies with a total of 12,378 HCC patients. It was shown that, patients with a higher pretherapeutic level of PLT had a significantly increased risk of VI (11 studies,8,759 patients; OR = 1.44, 95%CI: 1.02–2.02) and extrahepatic metastasis (6 studies,8, 951 patients; OR = 2.51, 95% CI: 2.19–2.88) in comparison with patients with a lower PLT. Funnel plots and Begg's tests indicated that there were no significant publication biases. Conclusion: This meta-analysis shows that pretherapeutic elevated PLT is associated with an increased risk of VI and extrahepatic metastasis in HCC.
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- 2024
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3. FOXA1/MND1/TKT axis regulates gastric cancer progression and oxaliplatin sensitivity via PI3K/AKT signaling pathway
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Xiaosi Hu, Shuai Zhou, Haohao Li, Zehui Wu, Ye Wang, Lei Meng, Zhangming Chen, Zhijian Wei, Qing Pang, and Aman Xu
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Gastric cancer ,Meiotic nuclear divisions 1 ,Forkhead box protein A1 ,Transketolase ,PI3K/AKT signaling pathway ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Drug resistance is a main factor affecting the chemotherapy efficacy of gastric cancer (GC), in which meiosis plays an important role. Therefore, it is urgent to explore the effect of meiosis related genes on chemotherapy resistance. Methods The expression of meiotic nuclear divisions 1 (MND1) in GC was detected by using TCGA and clinical specimens. In vitro and in vivo assays were used to investigate the effects of MND1. The molecular mechanism was determined using luciferase reporter assay, CO-IP and mass spectrometry (MS). Results Through bioinformatics, we found that MND1 was highly expressed in platinum-resistant samples. In vitro experiments showed that interference of MND1 significantly inhibited the progression of GC and increased the sensitivity to oxaliplatin. MND1 was significantly higher in 159 GC tissues in comparison with the matched adjacent normal tissues. In addition, overexpression of MND1 was associated with worse survival, advanced TNM stage, and lower pathological grade in patients with GC. Further investigation revealed that forkhead box protein A1 (FOXA1) directly binds to the promoter of MND1 to inhibit its transcription. CO-IP and MS assays showed that MND1 was coexpressed with transketolase (TKT). In addition,TKT activated the PI3K/AKT signaling axis and enhanced the glucose uptake and lactate production in GC cells. Conclusions Our results confirm that FOXA1 inhibits the expression of MND1, which can directly bind to TKT to promote GC progression and reduce oxaliplatin sensitivity through the PI3K/AKT signaling pathway.
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- 2023
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4. Lactiplantibacillus plantarum and Saccharomyces cerevisiae—Fermented Coconut Water Alleviates Dextran Sodium Sulfate-Induced Enteritis in Wenchang Chicken: A Gut Microbiota and Metabolomic Approach
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Leijie Zheng, Zhe Han, Jiachao Zhang, Jiamu Kang, Congfa Li, Qing Pang, and Sixin Liu
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probiotic-fermented coconut water ,acute enteritis ,gut microbiota diversity ,metabolomics ,Wenchang chicken ,dextran sodium sulfate ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
In order to investigate the potential mechanisms of probiotic-fermented coconut water in treating enteritis, this study conducted a comprehensive analysis of the effects of probiotic intervention on the recovery from Dextran Sodium Sulfate-induced acute enteritis in Wenchang chicks. The analysis encompassed the assessment of growth performance, serum indicators, intestinal tissue structure, and metagenomic and metabolomic profiles of cecal contents in 60 Wenchang chicks subjected to intervention. This approach aimed to elucidate the impact of probiotic intervention on the recovery process from acute enteritis at both the genetic and metabolic levels in the avian model. The results revealed that intervention with Saccharomyces cerevisiae Y301 improved the growth rate of chicks. and intervention with Lactiplantibacillus plantarum MS2c regulated the glycerophospholipid metabolism pathway and reshaped the gut microbiota structure in modeling chicks with acute enteritis, reducing the abundance of potentially pathogenic bacteria from the Alistipes and increasing the abundance of potentially beneficial species from the Christensenellaceae. This intervention resulted in the production of specific gut metabolites, including Gentamicin C and polymyxin B2, recognized for their therapeutic effects on acute enteritis. The combined intervention of S. cerevisiae Y301 and L. plantarum MS2c not only enhanced growth performance but also mitigated intestinal wall damage and increased the abundance of gut metabolites such as gentamicin C and polymyxin B2, thereby mitigating symptoms of enteritis. Furthermore, this combined intervention reduced the levels of serum immune markers, including IL-10, IL-6, TNF-α, IFN-γ, and D-lactic acid, thus mitigating intestinal epithelial cell damage and promoting acute enteritis recovery. This study provides crucial insights into the mechanisms of action of probiotics and probiotic-fermented coconut water in acute enteritis recovery, offering new perspectives for sustainable farming practices for Wenchang chicken.
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- 2024
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5. YY1 activates EMI2 and promotes the progression of cholangiocarcinoma through the PI3K/Akt signaling axis
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Shuai Zhou, Kang Lin Qu, Jin Ang Li, Shi Lei Chen, Yi Gang Zhang, Chao Zhu, Hao Jin, Yong Wang, Qing Pang, and Hui Chun Liu
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EMI2 ,YY1 ,Cholangiocarcinoma ,PI3K/Akt signaling pathway ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Cholangiocarcinoma (CCA) is one of the deadliest cancers of the digestive tract. The prognosis of CCA is poor and the 5-year survival rate is low. Bioinformatic analysis showed that early mitotic inhibitor 2 (EMI2) was overexpressed in CCA but the underlying mechanism is not known. Methods The data on bile duct carcinoma from TCGA and GEO databases were used to detect the expression of EMI2. The transcription factors of EMI2 were predicted using JASPAR and PROMO databases. Among the predicted transcription factors, YY1 has been rarely reported in cholangiocarcinoma, and was verified using the luciferase reporter gene assay. RT-PCR was performed to predict the downstream pathway of EMI2, and PI3K/Akt was suspected to be associated with it. Subsequently, in vivo and in vitro experiments were conducted to verify the effects of silencing and overexpressing EMI2 and YY1 on the proliferation, invasion, and metastasis of the bile duct cancer cells. Results EMI2 was highly expressed in CCA. Silencing EMI2 inhibited the proliferation, invasion, and migration of CCA cells, arrested cell cycle in the G1 phase, and promoted of apoptosis. The luciferase reporter gene assay showed that YY1 bound to the promoter region of EMI2, and after silencing YY1, the expression of EMI2 decreased and the progression of CCA was inhibited. Moreover, key proteins in the PI3K/Akt signaling pathway decreased after silencing EMI2. Conclusion EMI2 may be one of the direct targets of YY1 and promotes the progression of CCA through the PI3K/Akt signaling pathway.
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- 2021
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6. In-Plane Strain Tuned Electronic and Optical Properties in Germanene-MoSSe Heterostructures
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Qing Pang, Hong Xin, Ruipeng Chai, Dangli Gao, Jin Zhao, You Xie, and Yuling Song
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germanene ,MoSSe monolayer ,heterostructures ,first-principles calculation ,Chemistry ,QD1-999 - Abstract
DFT calculations are performed to investigate the electronic and optical absorption properties of two-dimensional heterostructures constructed by Janus MoSSe and germanene. It is found that a tiny gap can be opened up at the Dirac point in both Ge/SMoSe and Ge/SeMoS heterostructures, with intrinsic high-speed carrier mobility of the germanene layer being well preserved. An n-type Schottky contact is formed in Ge/SMoSe, while a p-type one is formed in Ge/SeMoS. Compared to corresponding individual layers, germanene-MoSSe heterostructures can exhibit extended optical absorption ability, ranging from ultraviolet to infrared light regions. The position of the Dirac cone, the Dirac gap value as well as the position of the optical absorption peak for both Ge/SMoSe and Ge/SeMoS heterostructures can be tuned by in-plane biaxial strains. It is also predicted that a Schottky–Ohmic transition can occur when suitable in-plane strain is imposed (especially tensile strain) on heterostructures. These results can provide a helpful guide for designing future nanoscale optoelectronic devices based on germanene-MoSSe vdW heterostructures.
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- 2022
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7. Biliary stenting alone versus biliary stenting combined with 125I particles intracavitary irradiation for the treatment of advanced cholangiocarcinoma
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Qing Pang, Lei Zhou, Xiao-Si Hu, Yong Wang, Zhong-Ran Man, Song Yang, Wei Wang, Zhen Qian, Hao Jin, and Hui-Chun Liu
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Medicine ,Science - Abstract
Abstract We aimed to compare the efficacy of percutaneous transhepatic biliary stenting (PTBS) and PTBS combined with 125I particles implantation in the treatment of advanced extrahepatic cholangiocarcinoma (EHC). A total of 184 advanced EHC patients, who received PTBS (PTBS group) or PTBS combined with 125I particles implantation (PTBS + 125I group) from January 2012 to April 2017 in our department, were retrospectively reviewed. The improvement of jaundice and liver function was observed in both groups. The postoperative complications, risk of biliary re-obstruction, and overall survival (OS) were compared between the two groups. Amongst, 71 cases received PTBS and 113 had the additional implantation of 125I particles. The jaundice and liver function were significantly improved in all patients, especially in PTBS + 125I group. There was no significant difference in the risk of postoperative complications between the two groups. However, the risk of biliary re-obstruction significantly reduced in PTBS + 125I group (19.5% vs. 35.2%, p = 0.017). Kaplan Meier analysis showed that patients in PTBS + 125I group had a significantly better OS, both for hilar and distal cholangiocarcinoma. Univariate analysis demonstrated that preoperative levels of carbohydrate antigen 19-9 (CA19-9), total bilirubin, neutrophil count, lymphocyte count, and different therapeutic method were significant factors affecting OS. Multivariate analysis further identified the treatment of PTBS combined with 125I particles implantation as an independent protective prognostic factor (HR = 0.26, 95% CI: 0.17–0.39, p
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- 2019
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8. MicroRNA-217: A regulator of human cancer
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Shuai Zhou, Chao Zhu, Qing Pang, and Hui Chun Liu
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mircoRNA-217 ,Cancer ,Target gene ,oncomiR ,Tumor suppressor miRNA ,Therapeutics. Pharmacology ,RM1-950 - Abstract
As highly conserved non-coding RNAs of approximately 18–24 nucleotides, microRNAs (miRNAs) regulate the expression of target genes. Multiple studies have demonstrated that miRNAs participate in the regulation of human cancer. MircoRNA-217 (miR-217) participates in the regulation of various tumors by specifically binding target genes and post-transcriptional regulation. In recent years, there have been numerous reports about miR-217 in tumor progression. MiR-217 is known mainly as a tumor suppressor, although some studies have shown that it functions as an oncomiR. Here, we review the current research related to miR-217, including its role in tumor progression and the molecular mechanisms.
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- 2021
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9. Banxia Xiexin Decoction Ameliorates t-BHP-Induced Apoptosis in Pancreatic Beta Cells by Activating the PI3K/AKT/FOXO1 Signaling Pathway
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Li-juan Du, Bing Pang, Yu-meng Tan, Ya-nan Yang, Mei-zhen Zhang, Qing Pang, Min Sun, and Qing Ni
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Background. Banxia Xiexin Decoction (BXXD) reportedly regulates glycolipid metabolism and inhibits pancreatic β-cell apoptosis. This study is aimed at investigating the protective effect of BXXD on tert-butyl hydroperoxide- (t-BHP-) induced apoptosis in MIN6 cells and the underlying mechanisms. Methods. MIN6 cells were preincubated with BXXD or liraglutide (Li) with or without PI3K inhibitor LY294002 (LY) for 12 h, following which t-BHP was added to induce MIN6 cell apoptosis. The protective effects of BXXD on MIN6 cells were evaluated by detecting cell viability and proliferation and glucose-stimulated insulin secretion (GSIS). The antiapoptotic effects were evaluated by Hoechst 33342 staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay (TUNEL). Malondialdehyde and glutathione peroxidase content and superoxide dismutase activity were measured using commercial kits. The expression of PI3K/AKT/FOXO1 signaling pathway-related signal molecules, and that of apoptotic indicators Bax, P27, and Caspase-3, was quantified using western blotting. Results. Preincubation with BXXD significantly improved t-BHP-induced proliferation inhibition and apoptosis and enhanced GSIS. t-BHP induced the generation of reactive oxygen species and inhibited the activities of antioxidant enzymes, which could be neutralized by pretreatment with BXXD. BXXD promoted the phosphorylation of AKT and FOXO1 in t-BHP-induced MIN6 cells. Moreover, BXXD attenuated the expression of related apoptotic indicators Bax, P27, and Caspase-3. LY abolished these effects of BXXD. Conclusion. BXXD protected MIN6 cells against t-BHP-induced apoptosis and improved insulin secretory function through modulation of the PI3K/AKT pathway and the downstream FOXO1, thus suggesting a novel therapeutic approach for type 2 diabetes mellitus (T2DM).
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- 2020
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10. Albumin-Bilirubin Grade as a Novel Predictor of Survival in Advanced Extrahepatic Cholangiocarcinoma
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Yong Wang, Qing Pang, Hao Jin, Lei Zhou, Xiaosi Hu, Zhen Qian, Zhongran Man, Song Yang, and Huichun Liu
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Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Aim. Child-Pugh (CP) grade has been used to assess liver function and postoperative outcomes in biliary tract neoplasms. The aim of this study was to preliminarily explore the prognostic significance of an alternative model of liver function, called albumin-bilirubin (ALBI) grade, in patients with extrahepatic cholangiocarcinoma (EHC). Methods. A total of 109 advanced EHC patients, who received percutaneous transhepatic biliary stenting combined with iodine-125 seed implantation from January 2012 to April 2017 in our department, were enrolled. Preoperative clinical data were collected to calculate the CP and ALBI grades. The performance of ALBI score in predicting postoperative death was compared with that of CP score by using the receiver operating characteristic (ROC) curve. Kaplan-Meier analysis and Cox regression model were performed for overall survival (OS) analysis. Results. The median survival time of our cohort was 12 months, and the 1-year and 2-year survival rates were 56.9% and 12.8%, respectively. The area under the ROC curve of ALBI score for predicting death was significantly greater than the CP score (0.751, 95% CI: 0.641–0.861, P
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- 2018
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11. Survival Analysis of Hepatocellular Carcinoma: A Comparison Between Young Patients and Aged Patients
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Xin-Sen Xu, Wei Chen, Run-Chen Miao, Yan-Yan Zhou, Zhi-Xin Wang, Ling-Qiang Zhang, Kai Qu, Qing Pang, Rui-Tao Wang, and Chang Liu
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Age ,Hepatocellular Carcinoma ,Multivariate Analysis ,Prognosis ,Medicine - Abstract
Background: To compare the clinicopathological features and prognosis between younger and aged patients with hepatocellular carcinoma (HCC). Methods: We analyzed the outcome of 451 HCC patients underwent liver resection, transcatheter arterial chemoembolization and radiofrequency ablation, respectively. Then risk factors for aged and younger patients′ survival were evaluated by multivariate analysis, respectively. Results: The patients who were older than 55 years old were defined as the older group. The overall survival for aged patients was significantly worse than those younger patients. The younger patients had similar liver functional reserve but more aggressive tumor factors than aged patients. Cox regression analysis showed that the elevated levels of aspartate aminotransferase (AST) (Wald χ2 = 3.963, P = 0.047, hazard ratio [HR] =1.453, 95% confidence interval [CI]: 1.006-2.098), lower albumin (Wald χ2 = 12.213, P < 0.001, HR = 1.982, 95% CI: 1.351-2.910), tumor size (Wald χ2 = 8.179, P = 0.004, HR = 1.841, 95% CI: 1.212-2.797), and higher alpha-fetoprotein level (Wald χ2 = 4.044, P = 0.044, HR = 1.465, 95% CI: 1.010-2.126) were independent prognostic factors for aged patients, while only elevated levels of AST (Wald χ2 = 14.491, P < 0.001, HR = 2.285, 95% CI: 1.493-3.496) and tumor size (Wald χ2 = 21.662, P < 0.001, HR = 2.928, 95% CI: 1.863-4.604) were independent prognostic factors for younger patients. Conclusions: Age is a risk factor to determine the prognosis of patients with HCC. Aged patients who have good liver functional reserve are still encouraged to receive curative therapy.
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- 2015
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12. 5-HT Drives Mortality in Sepsis Induced by Cecal Ligation and Puncture in Mice
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Jingyao Zhang, Jianbin Bi, Sushun Liu, Qing Pang, Ruiyao Zhang, Shun Wang, and Chang Liu
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Pathology ,RB1-214 - Abstract
Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection with a high mortality. 5-Hydroxytryptamine (5-HT) is an important regulatory factor in inflammation. The aim of this study is to investigate the role of 5-HT on cecal ligation and puncture- (CLP-) induced sepsis in the mouse model. CLP was performed on C57B/6 wild-type (WT) mice and tryptophan hydroxylase 1 (TPH1) knockout (KO) mice. The results showed that the 5-HT-sufficient group mice had a significantly lower survival rate than the 5-HT-deficient group in CLP-induced sepsis and septic shock. The KO-CLP sepsis group received a lower clinical score than the WT-CLP sepsis group. Meanwhile, the body temperature of mice in the KO-CLP sepsis group was higher than that in the WT-CLP sepsis group and was much closer to the normal body temperature 24 hours after CLP. The tissue histopathology analysis revealed that 5-HT markedly exacerbated histological damages in the peritoneum, lung, liver, kidney, intestinal tissue, and heart in sepsis. Moreover, significant lower levels of TNF-α, IL-6, bacterial loads, MPO, and ROS were discovered in the KO-CLP sepsis group in contrast to the WT-CLP sepsis group. In conclusion, 5-HT drives mortality and exacerbates organ dysfunction by promoting serum cytokines and bacterial loads as well as facilitating oxidative stress in the process of sepsis.
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- 2017
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13. Characterization of Nanosized Al2O3 Powder Synthesized by Thermal-Assisted MOCVD and Plasma-Assisted MOCVD
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Han Shizhong, Chen Jinghui, Zheng Peng,, and Qing Pang
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nanosized ,al2o3 ,thermal mocvd ,plasma ,Chemical engineering ,TP155-156 ,Chemistry ,QD1-999 - Abstract
Nanosized Al2O3 powder is synthesized by thermal Metal Organic Chemical Vapor Deposition (MOCVD)combined withplasma. The effects of reaction temperature, pressure, Al(CH3)3 (TMA) concentration and reactant gases (CO2 and O2) on the characteristics of the synthesized Al2O3 powders are investigated. The experimental results demonstrate that very fineAl2O3 powders with mean particle size of about 2.5 nm can be obtained at 5.3kPareactor pressure and 1000 oCby the thermal MOCVD. As the pressure is increased from 5.3kPa to 100 kPa, the mean diameter of Al2O3 powders also reaches to 10 nm. In other words, the increase in pressure has a negative effect on the synthesis of nanosized Al2O3. Meanwhile, it is also observed that the increment of temperature can promote the synthesis of fine Al2O3 powder.
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- 2011
14. Data Reduction for Noisy Data Classification Using Semi-supervised Manifold-Preserving Graph Reduction.
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Li Zhang 0004, Qing-Qing Pang, Zhiqiang Xu, and Xiaohan Zheng
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- 2020
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15. Forward Iterative Feature Selection Based on Laplacian Score.
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Qing-Qing Pang and Li Zhang 0004
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- 2020
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16. Fast Backward Iterative Laplacian Score for Unsupervised Feature Selection.
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Qing-Qing Pang and Li Zhang 0004
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- 2020
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17. A recursive feature retention method for semi-supervised feature selection.
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Qing-Qing Pang and Li Zhang 0004
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- 2021
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18. Fast Gaussian kernel support vector machine recursive feature elimination algorithm.
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Li Zhang 0004, Xiaohan Zheng, Qing-Qing Pang, and Weida Zhou
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- 2021
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19. Uncovering Chemical Interactions between Danshen and Danggui Using Liquid Chromatography–Mass Spectrometry and Network Pharmacology‑Based Research on Stroke.
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Han-Qing Pang, Jia-Xiu Guo, Xiao-Yu Shang, Jian Wang, Hui Liu, Jing Tian, Lu Zhang, Jian-Bin Wang, Wei Shi, Bing-Chun Yan, and Liang Liu
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- 2024
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20. Mn2+-Activated Photostimulable Persistent Nanophosphors by Pr3+ Codoping for Rewritable Information Storage
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Feng Gao, Qing Pang, Dangli Gao, Chaoyang Jia, Hong Xin, Yong Pan, Yuhua Wang, and Sining Yun
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General Materials Science - Published
- 2023
21. Metabonomic Method to Analyze the Changes in Pancreatic metabolites in Tree Shrew Traumatic Systemic Inflammatory Response Syndrome After Treatment with Umbilical Cord Mesenchymal Stem Cells
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Guang-Ping Ruan, Xiang Yao, Kai Wang, Shu-Qian Lin, Rong-Qing Pang, and Xing-Hua Pan
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General Veterinary ,Animal Science and Zoology - Abstract
Background: The impact method was used to make unilateral femoral comminuted fractures in tree shrews, which were then intravenously injected with lipopolysaccharide to create a traumatic systemic inflammatory response syndrome model. Methods: The treatment group was infused with tree shrew umbilical cord mesenchymal stem cells via the tail vein. After 10 days of treatment, 6 tree shrews were killed in the control, model and treatment groups and pancreatic tissue was collected for metabonomic analysis. Result: The 25 differential metabolites in the normal, model and stem cell treatment groups showed a trend of first decreasing and then increasing and 11 metabolites showed a trend of first increasing and then decreasing. These findings are in line with the recovery of the curative effect of the stem cell therapy model group.
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- 2023
22. Trap‐Dependent Optical/Thermal Stimulated Luminescence of Gallate Phosphors Charged by UV–visible–NIR light for Multiplexed Data Storage
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Dangli Gao, Zhigang Wang, Qing Pang, Qingqing Kuang, Feng Gao, Xiangyu Zhang, Sining Yun, and Xiaojun Wang
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Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Published
- 2023
23. High Efficiency Photocatalyst with Ultra‐Fine Pd NPs Constructed at Room Temperature for CO 2 Reduction
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Qing‐Qing Pang and Xi‐Zheng Fan
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General Chemistry - Published
- 2023
24. Ultra-fine Ru nanoparticles decorated V2O3 as a pH-universal electrocatalyst for efficient hydrogen evolution reaction
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Xi-Zheng Fan, Qing-Qing Pang, Fan Fan, Hong-Chang Yao, and Zhong-Jun Li
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Fuel Technology ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,Condensed Matter Physics - Published
- 2023
25. Effects of Addition of Titanium on Microstructures and Properties of Laser Butt Welded Joints of Mo–30W Alloy
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Pei-Xin Cheng, Lin-Jie Zhang, Jie Ning, Suck-Joo Na, and Yong-qing Pang
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science - Published
- 2022
26. In Situ Construction of Bifunctional N-Doped Carbon-Anchored Co Nanoparticles for OER and ORR
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Xi-Zheng Fan, Xin Du, Qing-Qing Pang, Shuo Zhang, Zhong-Yi Liu, and Xin-Zheng Yue
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General Materials Science - Published
- 2022
27. Tuning multicolour emission of Zn2GeO4:Mn phosphors by Li+ doping for information encryption and anti-counterfeiting applications
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Zihan Zhang, Qing Pang, Xiangyu Zhang, Dangli Gao, Peng Wang, Huan Jiao, Kaiwei Ma, and Xin Hong
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Inorganic Chemistry ,Materials science ,Photoluminescence ,business.industry ,Annealing (metallurgy) ,Doping ,Optoelectronics ,Phosphor ,business ,Luminescence ,Excitation ,Hydrothermal circulation ,Afterglow - Abstract
Traditional fluorescent materials used in anti-counterfeiting field usually exhibit monochromatic luminescence at a single-wavelength excitation, which is easily be forged by sophisticated counterfeiters. In this work, Zn2GeO4:Mn, x%Li (x=0 and 20), Zn2GeO4NaLiGe4O9:Mn, x%Li (x=50 and 70) and NaLiGe4O9:Mn micro-phosphors with the multi-chromatic and multi-mode luminescence have been successfully synthesized via a hydrothermal approach followed by an annealing treatment. As expected that these Li+ doped Zn2GeO4:Mn and NaLiGe4O9:Mn phosphors exhibit a double peak emission including a long green afterglow (~540 nm) and a red photoluminescence (∼668 nm). By tuning Li+ doping concentrations, a gradual colour output and a tuneable afterglow duration are achieved. Particularly, the Zn2GeO4:Mn,Li and NaLiGe4O9:Mn phosphors exhibit excellent performance as security inks for printing luminescent numbers and anti-counterfeiting patterns, which shows an afterglow time-dependent or excitation wavelength-dependent luminescence colour evolution. This work proves the feasibility of Li+ doping strategy in emission tuning, which can stimulate further studies on multi-mode luminescent materials in anti-counterfeiting applications.
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- 2022
28. Semi-supervised neighborhood discrimination index for feature selection.
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Qing-Qing Pang and Li Zhang 0004
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- 2020
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29. Erythroid-transdifferentiated myeloid cells promote portal vein tumor thrombus in hepatocellular carcinoma.
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Wei-Hang Zhu, Jie Chen, Run-Kai Huang, Yuan Zhang, Ze-Xuan Huang, Xiu-Qing Pang, Bo Hu, Yang Yang, and Xing Li
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- 2023
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30. Extending the color response range of Yb3+ concentration-dependent multimodal luminescence in Yb/Er doped fluoride microrods by annealing treatment
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Wei Chen, Qing Pang, Dangli Gao, Rui-peng Chai, Dan Zhao, Yong Pan, and Xiangyu Zhang
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Range (particle radiation) ,Materials science ,business.industry ,Annealing (metallurgy) ,Process Chemistry and Technology ,Doping ,Photon upconversion ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Downshifting ,chemistry.chemical_compound ,chemistry ,Materials Chemistry ,Ceramics and Composites ,Optoelectronics ,business ,Luminescence ,Fluoride ,Excitation - Abstract
For anti-counterfeiting and full-color display applications, adjusting the Yb3+ concentrations or excitation conditions is the basic and simple way to control luminescence color and intensity. However, this approach is significantly limited because the luminescence color is not very sensitive to the response of doped Yb3+ concentrations or excitation conditions. Herein, we have demonstrated that the as-synthesized NaLuF4:Yb/Er and their annealed counterpart LuOF:Yb/Er microrods exhibit upconversion/downshifting double-mode luminescence with complementary and rich colors from green, yellow to red under 980/365/488 nm excitation. The anti-counterfeiting patterns printed with these microrods inks have the ability of the color response to the excitation wavelength, excitation power and laser scanning speed, which make them be hard to duplicate. This study indicates that Yb/Er doping NaLuF4 and their complementary LuOF microrods have great potential in multilevel anti-counterfeiting application and display. In addition, heat treatment way also brings novel ideas to the synthesis of high efficient luminescence materials.
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- 2021
31. Effects and mechanisms of mUCMSCs on ovarian structure and function in naturally ageing C57 mice
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Jie He, Zhu Xiangqing, Ni-Ni Tian, Xiang Yao, Xue-Juan Zhang, Jing Zhao, Xue-Min Cai, Rong-qing Pang, Xing-hua Pan, Zai-Ling Yang, Kai Wang, and Ruan Guangping
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Granulosa cells ,SOD2 ,Biology ,Andrology ,Mice ,Animals ,Endocrine system ,chemistry.chemical_classification ,Follicles ,Reactive oxygen species ,Research ,Ovary ,Mesenchymal stem cell ,Obstetrics and Gynecology ,Gynecology and obstetrics ,Antral follicle ,Ovarian senescence ,mUCMSC ,Transplantation ,Oncology ,chemistry ,Ageing ,Apoptosis ,Models, Animal ,RG1-991 ,Female ,Gene expression - Abstract
Background The ovaries are the core reproductive organs in women and are critical for maintaining normal reproductive function and endocrine system stability. An ageing C57 mouse model was used to evaluate the efficacy and mechanism of mouse umbilical cord mesenchymal stem cells (mUCMSCs) and to explore the mechanism by which mUCMSCs promote the antioxidant repair of mouse granulosa cells (mGCs). Results Eighteen-month-old C57 mice were randomly divided into a model group and a treatment group. At the same time, 2-month-old C57 mice were established as a young group (15 mice per group). The mice in the treatment group were injected via the tail vein with GFP-labelled mUCMSCs. The ovarian volume in ageing C57 mice was decreased, and there were no follicles at any stage. After mUCMSC transplantation, the mouse ovaries increased in size, follicles at various stages were observed in the cortex, and the antral follicle counts increased. The serum E2, AMH, and INH-B levels of mice in the treatment group were significantly higher than those of mice in the model control group (P Conclusions mUCMSCs play roles in promoting the repair of ageing ovaries by regulating immunity, anti-inflammatory responses and the PI3K-Akt signalling pathway.
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- 2021
32. Serum hsa_circ_0000615 is a prognostic biomarker of sorafenib resistance in hepatocellular carcinoma
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Lunjun Zhang, Tao Xu, Yuanyuan Li, Qing Pang, and Xiaolin Ding
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Microbiology (medical) ,Carcinoma, Hepatocellular ,Biochemistry (medical) ,Clinical Biochemistry ,Liver Neoplasms ,Public Health, Environmental and Occupational Health ,Hematology ,RNA, Circular ,Sorafenib ,Prognosis ,Medical Laboratory Technology ,Biomarkers, Tumor ,Immunology and Allergy ,Humans - Abstract
Circular RNAs (circRNAs) can shape tumor progression and chemoresistance. How specific circRNAs shape hepatocellular carcinoma (HCC) chemoresistance, however, remains to be fully elucidated.In total, serum samples were collected from 202 HCC patients that had completed four sorafenib chemotherapy cycles. Serum hsa_circ_0000615 levels in these patients were quantified via quantitative real-time polymerase chain reaction (qRT-PCR), with demographic details and survival outcomes being recorded for subsequent analyses.We found hsa_circ_0000615 to be significantly upregulated in chemoresistant HCC patients relative to chemosensitive patients, with such upregulation being positively correlated with disease stage. Moreover, the area under the curve (AUC) value for hsa_circ_0000615 was moderately good, and high levels of hsa_circ_0000615 expression were associated with shorter overall survival among chemoresistant HCC patients.Our results highlight hsa_circ_0000615 as a promising driver of sorafenib resistance in HCC patients, highlighting it as a promising target for the treatment of this deadly cancer type.
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- 2022
33. 125I Intracavitary Irradiation Combined with 125I Seeds Implantation for Treatment of Locally Advanced Pancreatic Head Cancer: A Retrospective Analysis of 67 Cases
- Author
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Hao Jing, Qing Pang, Shi Lei Chen, Yong Wang, Hui Chun Liu, Jin Ang Li, Kang Lin Qu, Shuai Zhou, and Chao Zhu
- Subjects
medicine.medical_specialty ,metal biliary stent ,business.industry ,Therapeutic effect ,Intracavitary irradiation ,Locally advanced ,International Journal of General Medicine ,General Medicine ,030204 cardiovascular system & hematology ,Jaundice ,medicine.disease ,locally advanced pancreatic cancer ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Pancreatic cancer ,Carcinoma ,Retrospective analysis ,125I ,Medicine ,Radiology ,Liver function ,medicine.symptom ,business ,Original Research - Abstract
Shuai Zhou, Chao Zhu, Shi Lei Chen, Jin Ang Li, Kang Lin Qu, Hao Jing, Yong Wang, Qing Pang, Hui Chun Liu Department of Hepatobiliary Pancreatic Surgery, Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital of Bengbu Medical College, Anhui, 233000, People’s Republic of ChinaCorrespondence: Hui Chun Liu; Qing PangDepartment of Hepatobiliary Pancreatic Surgery, Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital of Bengbu Medical College, Anhui, 233000, People’s Republic of ChinaEmail liuhcdoctor@126.com; portxiu2@126.comBackground: Pancreatic cancer is an aggressive malignant tumor of the digestive system and the fourth leading cause of tumor-related death. Intracavitary 125I seed irradiation has been recently developed as a therapy for locally advanced pancreatic head carcinoma. However, there are still many limitations, and more investigations are needed in order to optimize this new treatment method.Methods: Sixty-seven patients were included in our study; 41 cases treated by SEMS-CL-125I intracavular irradiation (SEMS-CL-125I group) and 26 cases treated by SEMS-CL-125I intracavular irradiation combined with 125I particle implantation in the tumor body (the combined group). Among the 67 patients, 43 were males and 24 were females, with an average age of 69.64± 8.84 years. Tumor site size was determined based on the MRI or CT imaging scans, and the number and radius of 125I particle placement were calculated according to a specific formula. 125I particles were inserted into the tumor with a radius of 1.5 cm and a row spacing of 1 cm. The main postoperative biochemical indexes, imaging analysis, postoperative analgesia degree, median survival time and rate of complications were compared between the two groups.Results: Jaundice and liver function improved in both groups after treatment for 6 months. The combined group did better. Kaplan–Meier analysis showed that patients in the combined group had a significantly better overall survival than those in the SEMS-CL-125I group. Patients in the combined group had less complications than those in the SEMS-CL-125I group (23.1% vs 34.1%), and the postoperative pain status of the combined group was improved (26.8% vs 53.8%).Conclusion: Compared with the SEMS-CL-125I intracavular irradiation alone, the combination of 125I seed implantation with solid tumor 125I seed implantation had a better therapeutic effect in LAPHC patients, with improved biochemical indicators, survival prognosis, pain relief, and fewer complications.Keywords: locally advanced pancreatic cancer, 125I, metal biliary stent
- Published
- 2021
34. Facet modulation of nickel-ruthenium nanocrystals for efficient electrocatalytic hydrogen evolution
- Author
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Qing-Qing Pang, Xue Bai, Xin Du, Shuo Zhang, Zhong-Yi Liu, and Xin-Zheng Yue
- Subjects
Biomaterials ,Colloid and Surface Chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
Constructing highly active electrocatalysts towards hydrogen evolution reaction (HER) in both alkaline and acidic media is essential for achieving a sustainable energy economy. Here, a facile ethylene glycol reduction strategy was employed to design the nickel-ruthenium nanocrystals (Ni-Ru NC) with an exposed highly active Ru (101) facet as an efficient electrocatalyst for HER. Testings show Ni-Ru NC outperforms the benchmark catalyst Pt/C by delivering extraordinarily low overpotentials of 21.1 and 70.9 mV to drive 10 mA cm
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- 2022
35. Research Progress on New Methods to Prevent and Treat Ovarian Senescence: A Review
- Author
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Kai Wang, Xiang Yao, Rong-Qing Pang, Xiang-Qing Zhu, Xing-Hua Pan, and Guang-Ping Ruan
- Subjects
General Veterinary ,Animal Science and Zoology - Abstract
Ovarian senescence is a special type of organ senescence and the ovaries are the earliest aging organs. The ovaries have approximately 1 million to 2 million follicles at birth, but only approximately 1000 primordial follicles are left in menopause. Ovarian function also decreases with age. Women’s fertility also declines. The ovaries are the core female reproductive organs and are of great significance for maintaining reproductive system function and endocrine stability. Ovarian aging is also considered an indicator of female body aging, which drives the aging of many organs of the body. Therefore, how to prevent and treat ovarian aging has become a research question that has been widely studied by biomedical scientists and geriatric researchers in recent years. Recently, studies have shown that bone marrow mesenchymal stem cells (BMSCs) can prevent and treat ovarian aging. This article reviews the characteristics of ovarian aging, the advantages and disadvantages of various clinical treatment measures and the advantages of bone marrow mesenchymal stem cell therapy, aiming to provide references for the prevention and treatment of ovarian aging. My article was written at the Basic Medical Laboratory of the 920th Hospital of the Joint Logistics Support Force of PLA and written from 2021 to 2022.
- Published
- 2022
36. HS1BP3, transcriptionally regulated by ESR1, promotes hepatocellular carcinoma progression
- Author
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Xiaosi Hu, Hongtao Pan, Shuai Zhou, Qing Pang, Yong Wang, Chao Zhu, Huichun Liu, Hao Jin, and Aman Xu
- Subjects
Gene Expression Regulation, Neoplastic ,Carcinoma, Hepatocellular ,Cell Movement ,Cell Line, Tumor ,Liver Neoplasms ,Biophysics ,Humans ,Apoptosis ,Cell Biology ,Molecular Biology ,Biochemistry ,G1 Phase Cell Cycle Checkpoints ,Cell Proliferation - Abstract
To explore the role of HS1-binding protein 3 (HS1BP3) in hepatocellular carcinoma (HCC) and the potential mechanism.The effect of HS1BP3 in the prognosis of HCC was analyzed. The influence of HS1BP3 silence on proliferation, migration, cell cycle, and apoptosis of HCC cells (Huh-7 and Sun-449) were evaluated. The upstream transcription factors of HS1BP3 were further explored.The high expression of HS1BP3 in HCC was significantly associated with poor prognosis. The silencing of HS1BP3 inhibited proliferation, invasion, migration, and promoted G1 phase cell cycle arrest and apoptosis of HCC cells. Estrogen receptors 1 (ESR1) inhibited proliferation and improved the prognosis of HCC via fusion with HS1BP3 promoter.HS1BP3 may serve as a novel tumor-promoting factor transcriptionally regulated by ESR1.
- Published
- 2022
37. LAG3+ erythroid progenitor cells inhibit HBsAg seroclearance during finite pegylated interferon treatment through LAG3 and TGF-β
- Author
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Xiu-Qing Pang, Xing Li, Wei-Hang Zhu, Run-Kai Huang, Zhi-shuo Mo, Ze-Xuan Huang, Yuan Zhang, Dong-Ying Xie, and Zhi-Liang Gao
- Subjects
Pharmacology ,Virology - Published
- 2023
38. Depletion of serotonin relieves concanavalin A-induced liver fibrosis in mice by inhibiting inflammation, oxidative stress, and TGF-β1/Smads signaling pathway
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Shuangchi Liu, Yong Wang, Zheng Lu, Huichun Liu, Hao Jin, Qing Pang, Yi Tan, and Mengnan Dai
- Subjects
Liver Cirrhosis ,Male ,0301 basic medicine ,Serotonin ,medicine.medical_specialty ,Smad Proteins ,Inflammation ,Tryptophan Hydroxylase ,Toxicology ,medicine.disease_cause ,Proinflammatory cytokine ,Transforming Growth Factor beta1 ,Superoxide dismutase ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Fibrosis ,Internal medicine ,Concanavalin A ,medicine ,Animals ,Humans ,Sirius Red ,Mice, Knockout ,TPH1 ,biology ,Chemistry ,General Medicine ,medicine.disease ,Mice, Inbred C57BL ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,biology.protein ,Mitogens ,medicine.symptom ,030217 neurology & neurosurgery ,Oxidative stress ,Signal Transduction - Abstract
Serotonin exerts important functions in several liver pathophysiological processes. In this study, we investigated the role of serotonin in concanavalin A (Con A)-induced liver fibrosis (LF) in mice and the underlying mechanisms. To establish the mouse model of LF, mice of wild-type (WT) and tryptophan hydroxylase 1 (Tph1) knockout (serotonin depletion) received Con A for 8 successive weeks. Degree of fibrosis was assessed by Sirius red staining, as well as the measurements of alpha smooth muscle actin (α- SMA), hydroxyproline (Hyp) and type I collagen in liver tissues. To elucidate the potential mechanisms, we assessed the effect of serotonin depletion on inflammatory, oxidative stress as well as TGF-β1/Smads signaling pathway. We found that serotonin depletion significantly inhibited collagen deposition as evaluated by less collagenous fiber in Sirus Red staining and reduced contents of Hyp and type I collagen. In addition, the absence of serotonin significantly inhibited the release of several inflammatory cytokines, including interleukin-6 (IL-6), interferon-gamma (IFN-γ), tumor necrosis-alpha (TNF-α), and transforming growth factor β1 (TGF-β1). Oxidative stress was also largely mitigated in LF mice with serotonin deficiency as manifested by the decreases of oxidative stress markers (malonaldehyde (MDA) and myeloperoxidase (MPO)), as well as the increases of antioxidant stress indicators (glutathione (GSH), and GSH-px, catalase (CAT), superoxide dismutase (SOD)) in liver tissues. Moreover, the lack of serotonin may provide an antifibrotic role by inhibiting the intrahepatic expressions of TGF-β1, phosphorylated-smad2 (p-smad2), and phosphorylated-smad3 (p-smad3). These results indicated that, serotonin depletion attenuates Con A-induced LF through the regulation of inflammatory response, oxidative stress injury, and TGF-β1/Smads signaling pathway.
- Published
- 2021
39. The Effect of Different Chicken Ovalbumin Extracts on Cell Proliferation
- Author
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Guang-ping Ruan, Xiang Yao, Kai Wang, Jie He, Xiang-qing Zhu, Rong-qing Pang, and Xing-hua Pan
- Subjects
General Veterinary ,Animal Science and Zoology - Abstract
Background: Protein extracts from chicken egg whites were prepared by utilizing different solvents and we studied the differences in proliferation. Methods: The egg white extract was prepared by using lysate, phosphate-buffered saline (PBS), saline and pure water. The in vitro mixture experiment was carried out to observe the effect of different egg white preparations on the proliferation of cells. The samples were divided into the following groups: the control group with media, the original lysate group, the new lysate group, the PBS group, the saline group and the pure water group. Result: The study found that at final concentrations of 10%, 20%, 30%, 40% and 50%, the differences among the six groups were statistically significant (P less than 0.01, F greater than 100). The results of pairwise comparison showed that the proliferative effect of chicken egg albumin extract prepared by PBS was significantly higher than that of the medium at final concentrations of 20%, 30% and 50% (P less than 0.05). When the fetal bovine serum concentration was only 8%, 7% and 5%, the cell proliferation effect was better than that of the control group with 10% fetal bovine serum, indicating that the chicken egg white extract promoted cell proliferation. This result indicated that the best chicken egg albumin extract was obtained through PBS addition. The quantity of expensive fetal bovine serum could be considerably reduced by supplementing the media with chicken egg albumin extract. Among the solvents tested, PBS was the best solvent for preparing chicken egg albumin extract.
- Published
- 2022
40. Lack of tumorigenesis and protumorigenic activity of human umbilical cord mesenchymal stem cells in NOD SCID mice
- Author
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Jie, He, Xiang, Yao, Ping, Mo, Kai, Wang, Zai-Ling, Yang, Ni-Ni, Tian, Xiang-Qing, Zhu, Jing, Zhao, Rong-Qing, Pang, Guang-Ping, Ruan, and Xing-Hua, Pan
- Subjects
Male ,Cancer Research ,Lymphoma, B-Cell ,Carcinogenesis ,Mesenchymal Stem Cells ,Mice, SCID ,Umbilical Cord ,Oncology ,Mice, Inbred NOD ,Models, Animal ,Tumor Cells, Cultured ,Genetics ,Animals ,Humans ,Female ,Neoplasm Metastasis ,HeLa Cells - Abstract
Background The tumorigenesis of infused umbilical cord mesenchymal stem cells (UC-MSCs) is being preclinically evaluated. Methods We observed tumor formation in NOD SCID mice after a single subcutaneous injection of hUC-MSCs and the effect of these cells on tumor growth in tumor-bearing mice. Three generations (P5, P7, and P10) of hUC-MSCs (1 × 107) from two donors (hUC-MSC1 and hUC-MSC2) were inoculated subcutaneously into NOD SCID mice. Subcutaneous transplantation models were established in NOD SCID mice with human cervical cancer HeLa cells (solid tumor) and human B cell lymphoma Raji cells (hematological tumor). Then, the animals were euthanized, gross dissection was performed, and tissues were collected. Various organs were observed microscopically to identify pathological changes and tumor metastasis. Results In the tumorigenesis experiment, no general anatomical abnormalities were observed. In the tumor promotion experiment, some animals in the HeLa groups experienced tumor rupture, and one animal died in each of the low- and medium-dose hUC-MSC groups. The results may have occurred due to the longer feeding time, and the tumor may have caused spontaneous infection and death. Pathological examination revealed no metastasis to distant organs in any group. In the Raji tumor model, some animals in each group experienced tumor rupture, and one animal in the medium-dose hUC-MSC group died, perhaps due to increased tumor malignancy. Thus, hUC-MSCs neither promoted nor inhibited tumor growth. No cancer cell metastasis was observed in the heart, liver, spleen, lungs, kidneys or other important organs, except that pulmonary venule metastasis was observed in 1 animal in the model group. Conclusions Injected hUC-MSCs were not tumorigenic and did not significantly promote or inhibit solid or hematological tumor growth or metastasis in NOD SCID mice.
- Published
- 2022
41. Chip Detection of Rabbit Peripheral Blood Mononuclear Cells and 293T Cells Induced by Chicken Egg White Extract Shows Significant Differences in the Signaling Pathways that Regulate the Pluripotency of Stem Cells
- Author
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Guang-ping Ruan, Xiang Yao, Ping Mo, Kai Wang, Jie He, Zai-ling Yang, Rong-qing Pang, Xiang-qing Zhu, and Xing-hua Pan
- Subjects
General Veterinary ,Animal Science and Zoology - Abstract
Background: Stem cell protein chips can detect stem cell-associated proteins expressed in cells. Methods: We isolated and cultured rabbit peripheral blood mononuclear cells (R-PBMCs) in two bottles, one containing medium and one containing medium with 40% chicken egg white extract. Similarly, 293T cells were also divided into two vials: one vial contained medium and one vial contained medium with 40% chicken egg white extract. After 3 days of culture, proteins were extracted from the cells and used for the stem cell protein chip. Result: Protein chip detection showed that 10 stem cell-associated proteins were increased in the rabbit peripheral blood mononuclear cells induced by chicken egg white extract. There were also 4 stem cell-associated proteins increased in the 293T cells induced by chicken egg white extract. The results of KEGG enrichment analysis indicated that peripheral blood mononuclear cells of rabbits were involved in the regulation of stem cell pluripotency before and after induction. Moreover, 293T cells were involved in the regulation of stem cell pluripotency before and after induction.
- Published
- 2022
42. Spectrum-Effect Relationship Analysis of Bioactive Compounds in Zanthoxylum nitidum (Roxb.) DC. by Ultra-High Performance Liquid Chromatography Mass Spectrometry Coupled With Comprehensive Filtering Approaches
- Author
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Si-wei Rao, Yuan-yuan Duan, Han-qing Pang, Shao-hua Xu, Shou-qian Hu, Ke-guang Cheng, Dong Liang, and Wei Shi
- Subjects
Pharmacology ,Pharmacology (medical) - Abstract
Zanthoxylum nitidum(Roxb.) DC. (ZN), with strong effects of anti-inflammation and antioxidant activities is treated as a core herb in traditional Chinese medicine (TCM) preparation for treating stomachache, toothache, and rheumatoid arthritis. However, the active ingredients of ZN are not fully clarified due to its chemical complexity. In the present study, a double spectrum–effect analysis strategy was developed and applied to explore the bioactive components in herbs, and ZN was used as an example. Here, the chemical components in ZN were rapidly and comprehensively profiled based on the mass defect filtering-based structure classification (MDFSC) and diagnostic fragment-ion-based extension approaches. Furthermore, the fingerprints of 20 batches of ZN samples were analyzed by high-performance liquid chromatography, and the anti-inflammatory and antioxidant activities of the 20 batches of ZN samples were studied. Finally, the partial least squares regression (PLSR), gray relational analysis models, and Spearman’s rank correlation coefficient (SRCC) were applied to discover the bioactive compounds in ZN. As a result, a total of 48 compounds were identified or tentatively characterized in ZN, including 35 alkaloids, seven coumarins, three phenolic acids, two flavonoids, and one lignan. The results achieved by three prediction models indicated that peaks4,12, and17were the potential anti-inflammatory compounds in ZN, whereas peaks3,5,7,12, and13were involved in the antioxidant activity. Among them, peaks4,5,7, and12were identified as nitidine, chelerythrine, hesperidin, and oxynitidine by comparison with the standards and other references. The data in the current study achieved by double spectrum–effect analysis strategy had great importance to improve the quality standardization of ZN, and the method might be an efficiency tool for the discovery of active components in a complex system, such as TCMs.
- Published
- 2022
43. Construction of a near-infrared fluorescent probe for ratiometric imaging of peroxynitrite during tumor progression
- Author
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Lu Jiao, Jinmao You, Qing Pang, and Li Zan
- Subjects
Diagnostic Imaging ,inorganic chemicals ,Fluorescence-lifetime imaging microscopy ,Biosensing Techniques ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Mice ,chemistry.chemical_compound ,In vivo ,Peroxynitrous Acid ,Electrochemistry ,Animals ,Humans ,Environmental Chemistry ,Spectroscopy ,Rapid response ,Fluorescent Dyes ,chemistry.chemical_classification ,Reactive oxygen species ,Optical Imaging ,021001 nanoscience & nanotechnology ,Fluorescence ,0104 chemical sciences ,Peroxynitrous acid ,chemistry ,Tumor progression ,cardiovascular system ,Biophysics ,Reactive Oxygen Species ,0210 nano-technology ,Peroxynitrite - Abstract
Malignant tumors are one of the main causes for human death and are tightly associated with overexpression of reactive oxygen species (ROS) in pathological processes. Therefore, in vivo monitoring of ROS, especially ONOO-, remains of great significance for diagnosis and therapy of tumors to improve the survival rate. Herein, we designed and constructed a reliable near-infrared (NIR) ratiometric fluorescent biosensor CDMS for monitoring the fluctuations of ONOO- in the process of tumor progression. CDMS featured outstanding stability to photoirradiation, substantial quantum yields, rapid response (
- Published
- 2021
44. Establishment of a Systemic Inflammatory Response Syndrome Model and Evaluation of the Efficacy of Umbilical Cord Mesenchymal Stem Cell Transplantation
- Author
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Xing-Hua Pan, Mi-Yang Liu-Gao, Rong-Qing Pang, Xiang Yao, Zi-an Li, Ping Mo, Kai Wang, Ruan Guangping, Ni-Ni Tian, Xue-Min Cai, Jin-Xiang Wang, and Zai-Ling Yang
- Subjects
Pathology ,medicine.medical_specialty ,Histology ,Lipopolysaccharide ,business.industry ,Mesenchymal stem cell ,H&E stain ,Mesenchymal Stem Cells ,Kidney ,Mesenchymal Stem Cell Transplantation ,medicine.disease ,Umbilical cord ,Systemic Inflammatory Response Syndrome ,Umbilical Cord ,Transplantation ,Systemic inflammatory response syndrome ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,In vivo ,medicine ,Humans ,Anatomy ,business ,Pathological - Abstract
Based on the characteristics of modern weapon injury, a repetitive model of traumatic systemic inflammatory response syndrome (SIRS) and an evaluation system were established. The models were treated with GFP-labeled tree shrew umbilical cord mesenchymal stem cells (UCMSCs). Forty out of 50 tree shrews were used to make a unilateral femoral comminuted fracture. Lipopolysaccharide was injected intravenously to create a traumatic SIRS model. The other 10 shrews were used as normal controls. After the model was established for 10 days, 20 tree shrews were injected intravenously with GFP-labeled UCMSCs, and 18 tree shrews were not injected as the model control group. The distribution of GFP-labeled cells in vivo was measured at 2 and 10 days after injection. Twenty days after treatment, the model group, the normal control group, and the treatment group were taken to observe the pathological changes in each tissue, and blood samples were taken for the changes in liver, renal, and heart function. Distribution of GFP-positive cells was observed in all tissues at 2 and 10 days after injection. After treatment, the HE staining results of the treatment group were close to those of the normal group, and the model group had a certain degree of lesions. The results of liver, renal, and heart function tests in the treatment group were returned to normal, and the results in the model group were abnormally increased. UCMSCs have a certain effect on the treatment of traumatic SIRS and provide a new technical solution for modern weapon trauma treatment.
- Published
- 2021
45. Insulin receptors regulate the fecundity of Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)
- Author
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Shigui Wang, Yan Li, Yu-Jia Luo, Yong-Kang Liu, Cai-Di Xu, Xiao-Qing Pang, Bin Tang, and Ying-Mei Deng
- Subjects
biology ,fungi ,biology.organism_classification ,Molecular biology ,Insulin receptor ,Vitellogenin ,RNA interference ,Insect Science ,biology.protein ,Tensin ,PTEN ,Glycogen synthase ,Delphacidae ,Protein kinase B - Abstract
Two InR (insulin receptor) genes have been identified in the Nilaparvata lugens. In this study, we used RNA interference (RNAi) to investigate the role of InR genes in the fecundity of N. lugens. The expression of NLInR1 and NLInR2 genes was simultaneously silenced with mixture of dsInR1 and dsInR2 (dsInRs) injection. Our results showed that larvae RNAi against both NLInR1 and NLInR2 reduced the number of eggs laid by N. lugens and some eggs as well as ovaries were abnormal. In addition, the relative expression of Vg (vitellogenin) and VgR (vitellogenin receptor) was significantly reduced on the 4th and 6th days after insects treated with larvae RNAi reached the adult stage. We also determined the relative expression levels of insulin/insulin-like signaling (IIS) related genes in RNAi-treated larvae and found that the expression levels of Chico (homologous receptor substrate), Akt (protein kinase B), PI3K (phosphoinositide 3-kinase), and PTEN (phosphatase and tensin homolog) genes decreased whereas FOXO (forkhead box O) and GSK3 (glycogen synthase kinase-3) levels increased on the 4th and 6th days after insects reached the adult stage. These results indicate that silencing of NLInR1 and NLInR2 reduces the fecundity of N. lugens through the IIS pathway.
- Published
- 2020
46. The Significance of Platelet–Albumin–Bilirubin (PALBI) Grade in Hepatocellular Carcinoma Patients Stratified According to Platelet Count
- Author
-
Yimin Lu, Chunfang Wang, Huadong Pan, Shuangchi Liu, Qing Pang, Huichun Liu, Lei Zhou, and Luyao Wang
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Thrombocytosis ,Proportional hazards model ,business.industry ,Cancer ,Retrospective cohort study ,medicine.disease ,Gastroenterology ,digestive system diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Internal medicine ,Hepatocellular carcinoma ,medicine ,Liver function ,Radical surgery ,business ,Liver cancer - Abstract
Background Platelet-albumin-bilirubin (PALBI) has been demonstrated to be superior to conventional Child-Pugh (C-P) grade in evaluating liver function and prognosis of HCC patients. However, both thrombocytosis and thrombocytopenia are unfavorable for HCC survival. The aim of this study was to preliminarily investigate the prognostic value of PALBI in HCC patients with thrombocytopenia and excluding thrombocytopenia. Methods In this retrospective cohort study, we reviewed 465 cases of HCC patients who underwent radical surgery. PALBI grade was calculated based on preoperative serological examinations. The primary outcomes were overall survival (OS) and recurrence-free survival (RFS), which were assessed by Kaplan-Meier method and Cox regression. The prognostic performances of PALBI and other models were estimated by using the concordance index (C-index). Results During a median follow-up time of 28 months, 31.6% (147/465) of patients died and 33.5% (156/465) experienced recurrence. Multivariate analyses revealed that both thrombocytosis and thrombocytopenia were independently associated with poor OS and RFS compared with normal platelet count (PLT) in HCC patients. Stratified analysis further revealed that PALBI was a significant predictor for HCC survival in patients excluding thrombocytopenia but not in patients with thrombocytopenia. In particular, in HCC patients excluding thrombocytopenia, the combination of tumor size with PALBI (C-index = 0.730, 95% CI: 0.674-0.786) may be superior to the classical Barcelona Clinic Liver Cancer (BCLC) and Cancer of Liver Italian Program (CLIP) staging systems in predicting survival. Conclusion In conclusion, PALBI grade, in particular the combination with tumor size, is an effective model for discriminating survival in HCC patients excluding thrombocytopenia but not in thrombocytopenic HCC patients.
- Published
- 2020
47. Low toxicity and high immunogenicity of an inactivated vaccine candidate against COVID-19 in different animal models
- Author
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Lei Zhang, Yan Ping Zhou, Ze Jun Wang, Shuo Shen, Xing-Lou Yang, Xin Wan, Xiaoming Yang, De Qing Pang, Zhengli Shi, Wei Ping Jin, Jie Wu, Jia Lu, Su Cai Zhang, Wenhui Wang, Ao Xiao, Zhiming Yuan, Kai Duan, Xin Guo Li, Miao Xu, Kang Wei Xu, Ulrich Desselberger, Yan Zhu, Chao Shan, Yun Xia Sun, Hua Jun Zhang, Jing Guo, Cheng Peng, Lie Fu, Hao Rui Si, Chang Gui Li, Lei You, Xiao Yu Zhang, Xiao Xiao Gao, Jing Liu, Sheng Li Meng, Jun Zhi Wang, An Na Yang, and Qian Cai
- Subjects
0301 basic medicine ,Male ,COVID-19 Vaccines ,Epidemiology ,030106 microbiology ,Immunology ,Population ,immunogenicity ,Antibodies, Viral ,Microbiology ,Virus ,03 medical and health sciences ,Immune system ,Virology ,Drug Discovery ,Medicine ,Animals ,Seroconversion ,education ,education.field_of_study ,biology ,business.industry ,SARS-CoV-2 ,Immunogenicity ,toxicity ,General Medicine ,inactivated vaccine ,animal models ,Immunity, Humoral ,030104 developmental biology ,Infectious Diseases ,Vaccines, Inactivated ,Toxicity ,Inactivated vaccine ,biology.protein ,Parasitology ,Female ,Antibody ,business ,Research Article - Abstract
The ongoing COVID-19 pandemic is causing huge impact on health, life, and global economy, which is characterized by rapid spreading of SARS-CoV-2, high number of confirmed cases and a fatality/case rate worldwide reported by WHO. The most effective intervention measure will be to develop safe and effective vaccines to protect the population from the disease and limit the spread of the virus. An inactivated, whole virus vaccine candidate of SARS-CoV-2 has been developed by Wuhan Institute of Biological Products and Wuhan Institute of Virology. The low toxicity, immunogenicity, and immune persistence were investigated in preclinical studies using seven different species of animals. The results showed that the vaccine candidate was well tolerated and stimulated high levels of specific IgG and neutralizing antibodies. Low or no toxicity in three species of animals was also demonstrated in preclinical study of the vaccine candidate. Biochemical analysis of structural proteins and purity analysis were performed. The inactivated, whole virion vaccine was characterized with safe double-inactivation, no use of DNases and high purity. Dosages, boosting times, adjuvants, and immunization schedules were shown to be important for stimulating a strong humoral immune response in animals tested. Preliminary observation in ongoing phase I and II clinical trials of the vaccine candidate in Wuzhi County, Henan Province, showed that the vaccine is well tolerant. The results were characterized by very low proportion and low degree of side effects, high levels of neutralizing antibodies, and seroconversion. These results consistent with the results obtained from preclinical data on the safety.
- Published
- 2020
48. The dependence of EPR g-factors on the local structure for tetragonal Nd3+ and Er3+ centers in CaF2 crystals
- Author
-
Rui-Peng Chai, Dang-Li Gao, Qing Pang, and Dan-Hui Hao
- Subjects
Physical and Theoretical Chemistry ,Condensed Matter Physics ,Biochemistry - Published
- 2023
49. Umbilical cord mesenchymal stem cells protect thymus structure and function in aged C57 mice by downregulating aging-related genes and upregulating autophagy- and anti-oxidative stress-related genes
- Author
-
Xiang Yao, Xing-Hua Pan, Qing-Keng Lin, Rong-Qing Pang, Zi-an Li, Ruan Guangping, and Xue-Min Cai
- Subjects
Senescence ,Aging ,Lymphocyte ,Regeneration (biology) ,Mesenchymal stem cell ,Cell Biology ,Biology ,Umbilical cord ,Transplantation ,Andrology ,medicine.anatomical_structure ,medicine ,Immunohistochemistry ,CD8 - Abstract
Background To study the effect of allogeneic umbilical cord mesenchymal stem cell transplantation on the structure and function of the thymus in aged C57 mice and provide a new method for the treatment of senile thymic atrophy. Results The changes in the thymus cortex and medulla volume and the lymphocyte ratio were analyzed by immunofluorescence. For thymus tissue sections, immunohistochemical staining was performed to detect p16, p53, SOD, becline1, LC3b, p62, sirt1, and sirt3. Changes in CK5, CK8, CD4 and CD8 expression were observed. Treatment with mUCMSCs could promote hair regeneration in aging mice and regenerate the thymus structure. Conclusions mUCMSCs inhibited senescence of the thymus and promoted structural and functional thymus regeneration by downregulating the senescence genes p53 and p16 and upregulating the SOD, Sirt1 and Sirt3 genes, but the mechanism requires further research. Methods C57 mice were obtained and met the requirements of thymic aging. mUCMSCs were infused via the tail vein at a dose of 1×107 cells/kg twice per week for 3 weeks. Six weeks after the last transplantation, the thymus was weighed, and the thymus-to-body weight ratio was calculated. The thymus tissue was stained with HE.
- Published
- 2020
50. Tuning multicolour emission of Zn
- Author
-
Dangli, Gao, Kaiwei, Ma, Peng, Wang, Xiangyu, Zhang, Qing, Pang, Hong, Xin, Zihan, Zhang, and Huan, Jiao
- Abstract
Traditional fluorescent materials used in the anti-counterfeiting field usually exhibit monochromatic luminescence at a single-wavelength excitation, which is easily forged by sophisticated counterfeiters. In this work, Zn
- Published
- 2021
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