1. Human Herpes Simplex Virus-1 depletes APOBEC3A from nuclei.
- Author
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Stewart JA, Holland TC, and Bhagwat AS
- Subjects
- Animals, Chlorocebus aethiops, Cytidine Deaminase genetics, Green Fluorescent Proteins analysis, Green Fluorescent Proteins genetics, HeLa Cells, Humans, Immunologic Factors genetics, Proteins genetics, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins genetics, Staining and Labeling, Vero Cells, Viral Proteins genetics, Cell Nucleus chemistry, Cytidine Deaminase analysis, Cytoplasm chemistry, Herpesvirus 1, Human growth & development, Immunologic Factors analysis, Proteins analysis, Viral Proteins biosynthesis
- Abstract
APOBEC3 family of DNA-cytosine deaminases inactivate and mutate several human viruses. We constructed a human cell line that is inducible for EGFP-tagged APOBEC3A and found A3A predominantly in the nuclei. When these cells were infected with Herpes Simplex Virus-1, virus titer was unaffected by A3A expression despite nuclear virus replication. When A3A expression and virus infection were monitored, A3A was found predominantly to be nuclear in infected cells up to 3 h post-infection, but was predominantly cytoplasmic by 12 h. This effect did not require the whole virus, and could be reproduced using the UL39 gene of the virus which codes for a subunit of the viral ribonucleotide reductase. These results are similar to the reported exclusion of APOBEC3B by Epstein Barr virus ortholog of UL39, BORF2, but HSV1 UL39 gene product appears better at excluding A3A than A3B from nuclei., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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