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Syncytial mutations in the herpes simplex virus type 1 gK (UL53) gene occur in two distinct domains.
- Source :
-
Journal of virology [J Virol] 1994 Dec; Vol. 68 (12), pp. 8277-81. - Publication Year :
- 1994
-
Abstract
- Syncytial (syn) mutants of herpes simplex virus cause cell fusion. Many syn mutations map to the syn1 locus, which has been identified with the gK (UL53) gene. In this work, the gK genes of eight syn mutants derived from the KOS strain were sequenced to identify residues and, possibly, domains important for the fusion activity of mutant gK. DNA sequencing showed that six mutants (syn30, syn31, syn32, syn102, syn103, and syn105) had single missense mutations in the gK gene. Two of these, syn31 and syn32, had identical mutations that caused the introduction of a potential site for N-linked glycosylation. syn31 gK was analyzed by in vitro translation and found to utilize the novel glycosylation site. Two other mutants, syn8 and syn33, had three mutations each, resulting in three amino acid substitutions in syn8 and two substitutions in syn33. Of the 10 gK syn mutant sequences known, 8 have mutations in the N-terminal domain of gK, suggesting that this domain, which is likely to be an ectodomain, is important for the function of the protein. The other two mutants, syn30 and syn103, have mutations near the C terminus of gK.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Chlorocebus aethiops
DNA Primers
DNA, Viral chemistry
DNA, Viral metabolism
Herpesvirus 1, Human metabolism
Molecular Sequence Data
Mutagenesis, Site-Directed
Polymerase Chain Reaction
Restriction Mapping
Transfection
Vero Cells
Viral Proteins metabolism
Genome, Viral
Herpesvirus 1, Human genetics
Point Mutation
Viral Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 68
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 7966620
- Full Text :
- https://doi.org/10.1128/JVI.68.12.8277-8281.1994