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Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes.
- Source :
-
International journal of obesity (2005) [Int J Obes (Lond)] 2007 Jan; Vol. 31 (1), pp. 78-86. Date of Electronic Publication: 2006 May 02. - Publication Year :
- 2007
-
Abstract
- Objective: Human adenovirus Ad-36 causes adiposity in animal models and shows association with human obesity. Ad-36 enhances differentiation of 3T3-L1 and human preadipocytes, without cell lysis, a characteristic that may contribute to its adipogenic effect observed in vivo. Ad-2, another human adenovirus is nonadipogenic in animals and in 3T3-L1 cells and shows no correlation with human obesity. The objective of this study was to determine the adipogenic roles of viral mRNA and DNA, which may explain the differential effects of Ad-36 and Ad-2 on preadipocyte differentiation.<br />Methods: This study determined the duration of selected Ad-36 gene expression in 3T3-L1 cells, and the effect on preadipocytes differentiation, when Ad-36 gene expression was attenuated by Cidofovir, an antiadenoviral agent.<br />Results: The results showed that Ad-36, but not Ad-2, expresses viral mRNA. Ad-36 gene expression peaked at 2-4 days postinoculation and very low levels persisted after day 7. Despite the viral mRNA expression, Ad-36 infection of 3T3-L1 cells was abortive as indicated by a progressive decrease in viral DNA quantity. Attenuation of Ad-36 mRNA expression by Cidofovir reduced the adipogenic effect of the virus.<br />Conclusion: In conclusion, viral mRNA expression, although transient, is a prerequisite for enhancing differentiation of preadipocytes by Ad-36. Viral DNA replication was not required for the effect. This is the first evidence for the role of gene expression of an adipogenic human virus in enhancing preadipocytes differentiation. This study provides the basis for further understanding novel regulatory modulators of preadipocytes differentiation.
- Subjects :
- 3T3-L1 Cells
Adenovirus E1A Proteins genetics
Adenovirus E4 Proteins genetics
Adipocytes drug effects
Adipogenesis drug effects
Adipogenesis genetics
Animals
Antiviral Agents pharmacology
CCAAT-Enhancer-Binding Protein-beta genetics
Cidofovir
Cytosine analogs & derivatives
Cytosine pharmacology
Gene Expression drug effects
Gene Expression genetics
Genes, Viral genetics
Humans
Mice
Obesity physiopathology
Obesity virology
Organophosphonates pharmacology
RNA, Viral genetics
Adenoviruses, Human genetics
Adipocytes physiology
Adipogenesis physiology
DNA Replication genetics
DNA, Viral genetics
RNA, Viral metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0307-0565
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of obesity (2005)
- Publication Type :
- Academic Journal
- Accession number :
- 16652125
- Full Text :
- https://doi.org/10.1038/sj.ijo.0803358