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251 results on '"Falasca, L."'

3. Fatal Takotsubo syndrome in critical COVID-19 related pneumonia

Author

Titi, L., Magnanimi, E., Mancone, M., Infusino, F., Coppola, G., Del Nonno, F., Colombo, D., Nardacci, R., Falasca, L., d&apos, Amati, G., Tarsitano, M. G., Merlino, L., Fedele, F., and Pugliese, F.

Subjects
0301 basic medicine, medicine.medical_specialty, Resuscitation, Autopsy, 030204 cardiovascular system & hematology, Myocardial rupture, Article, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Hematoma, autopsy, Internal medicine, Edema, Medicine, business.industry, Takotsubo Syndrome, Contraction band necrosis, COVID-19, COVID-19 related pneumonia, Stress cardiomyopathy, Aged, 80 and over, Biopsy, COVID-19 Nucleic Acid Testing, Fatal Outcome, Humans, Male, Myocardium, Takotsubo Cardiomyopathy, General Medicine, medicine.disease, Pneumonia, 030104 developmental biology, Coagulative necrosis, Cardiology, stress cardiomyopathy, medicine.symptom, business, Cardiology and Cardiovascular Medicine, COVID-19 related pneumonia, COVID-19
Abstract

Highlights • Takotsubo Syndrome (TTS) is increasingly reported in COVID-19 patients • The pathogenic mechanisms underlying TTS in this particular setting are still unknown • Autopsy findings in fatal cases may contribute to understand the possible role of SARS-CoV-2 in the onset of TTS, COVID-19 can involve several organs and systems, often with indirect and poorly clarified mechanisms. Different presentations of myocardial injury have been reported, with variable degrees of severity, often impacting on the prognosis of COVID-19 patients. The pathogenic mechanisms underlying cardiac damage in SARS-CoV-2 infection are under active investigation. We report the clinical and autopsy findings of a fatal case of Takotsubo Syndrome occurring in an 83-year-old patient with COVID-19 pneumonia. The patient was admitted to Emergency Department with dyspnea, fever and diarrhea. A naso-pharyngeal swab test for SARS-CoV-2 was positive. In the following week his conditions worsened, requiring intubation and deep sedation. While in the ICU, the patient suddenly showed ST segment elevation. Left ventricular angiography showed decreased with hypercontractile ventricular bases and mid-apical ballooning, consistent with diagnosis of Takotsubo syndrome (TTS). Shortly after the patient was pulseless. After extensive resuscitation maneuvers, the patient was declared dead. Autopsy revealed a subepicardial hematoma, in absence of myocardial rupture. On histology, the myocardium showed diffuse edema, multiple foci of contraction band necrosis in both ventricles and occasional coagulative necrosis of single cardiac myocytes. Abundant macrophages CD68+ were detected in the myocardial interstitium. The finding of diffuse contraction band necrosis supports the pathogenic role of increased catecholamine levels; the presence of a significant interstitial inflammatory infiltrate, made up by macrophages, remains of uncertain significance.

Published
2021
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4. Dying “from” or “with” covid-19 during the pandemic: Medico-legal issues according to a population perspective

Author

De-Giorgio, F., Grassi, V. M., Bergamin, Eva, Cina, Alessandro, Del Nonno, F., Colombo, D., Nardacci, R., Falasca, L., Conte, Celeste, D'Aloja, E., Damiani, Gianfranco, Vetrugno, Giuseppe, Bergamin E., Cina A., Conte C., Damiani G. (ORCID:0000-0003-3028-6188), Vetrugno G. (ORCID:0000-0003-0181-2855), De-Giorgio, F., Grassi, V. M., Bergamin, Eva, Cina, Alessandro, Del Nonno, F., Colombo, D., Nardacci, R., Falasca, L., Conte, Celeste, D'Aloja, E., Damiani, Gianfranco, Vetrugno, Giuseppe, Bergamin E., Cina A., Conte C., Damiani G. (ORCID:0000-0003-3028-6188), and Vetrugno G. (ORCID:0000-0003-0181-2855)

Abstract

There is still a lack of knowledge concerning the pathophysiology of death among COVID-19-deceased patients, and the question of whether a patient has died with or due to COVID-19 is still very much debated. In Italy, all deaths of patients who tested positive for SARS-CoV-2 are defined as COVID-19-related, without considering pre-existing diseases that may either contribute to or even cause death. Our study included nine subjects from two different nursing homes (Cases 1–4, Group A; Cases 5–9, Group B). The latter included patients who presumably died from CO poisoning due to a heating system malfunction. All subjects tested positive for COVID-19 both ante-and post-mortem and were examined using post-mortem computed tomography prior to autopsy. COVID-19 was determined to be a contributing cause in the deaths of four out of nine subjects (death due to COVID-19; i.e., pneumonia and sudden cardiac death). In the other five cases, for which CO poisoning was identified as the cause of death, the infection presumably had no role in exitus (death with COVID-19). In our attempt to classify our patients as dying with or due to COVID-19, we found the use of complete assessments (both histological analyses and computed tomography examination) fundamental.

Published
2021

13. Transglutaminase type 2 plays a key role in the pathogenesis of Mycobacterium tuberculosis infection

Author

Palucci, Ivana, Matic, I., Falasca, L., Minerva, Mariachiara, Maulucci, Giuseppe, De Spirito, Marco, Petruccioli, E., Goletti, D., Rossin, F., Piacentini, M., Delogu, Giovanni, Palucci, I., Minerva, M., Maulucci, G. (ORCID:0000-0002-2154-319X), De Spirito, M. (ORCID:0000-0003-4260-5107), Delogu, G. (ORCID:0000-0003-0182-8267), Palucci, Ivana, Matic, I., Falasca, L., Minerva, Mariachiara, Maulucci, Giuseppe, De Spirito, Marco, Petruccioli, E., Goletti, D., Rossin, F., Piacentini, M., Delogu, Giovanni, Palucci, I., Minerva, M., Maulucci, G. (ORCID:0000-0002-2154-319X), De Spirito, M. (ORCID:0000-0003-4260-5107), and Delogu, G. (ORCID:0000-0003-0182-8267)

Abstract

Background: Mycobacterium tuberculosis (MTB), the aetiological agent of tuberculosis (TB), is capable of interfering with the phagosome maturation pathway, by inhibiting phagosome–lysosome fusion and the autophagic process to ensure survival and replication in macrophages. Thus, it has been proposed that the modulation of autophagy may represent a therapeutic approach to reduce MTB viability by enhancing its clearance. Objective: The aim of this study was to investigate whether transglutaminase type 2 (TG2) is involved in the pathogenesis of MTB. Results: We have shown that either genetic or pharmacological inhibition of TG2 leads to a marked reduction in MTB replicative capacity. Infection of TG2 knockout mice demonstrated that TG2 is required for MTB intracellular survival in macrophages and host tissues. The same inhibitory effect can be reproduced in vitro using Z-DON, a specific inhibitor of the transamidating activity of TG2. Massive cell death observed in macrophages that properly express TG2 is hampered by the absence of the enzyme and can be largely reduced by the treatment of wild-type macrophages with the TG2 inhibitor. Our data suggest that reduced MTB replication in cells lacking TG2 is due to the impairment of LC3/autophagy homeostasis. Finally, we have shown that treatment of MTB-infected murine and human primary macrophages with cystamine, a TG2 inhibitor already tested in clinical studies, causes a reduction in intracellular colony-forming units in human macrophages similar to that achieved by the anti-TB drug capreomycin. Conclusion: These results suggest that inhibition of TG2 activity is a potential novel approach for the treatment of TB.

Published
2018

17. Longitudinal characterization of dysfunctional T cell-activation during human acute Ebola infection

Author

Agrati, C, primary, Castilletti, C, additional, Casetti, R, additional, Sacchi, A, additional, Falasca, L, additional, Turchi, F, additional, Tumino, N, additional, Bordoni, V, additional, Cimini, E, additional, Viola, D, additional, Lalle, E, additional, Bordi, L, additional, Lanini, S, additional, Martini, F, additional, Nicastri, E, additional, Petrosillo, N, additional, Puro, V, additional, Piacentini, M, additional, Di Caro, A, additional, Kobinger, G P, additional, Zumla, A, additional, Ippolito, G, additional, and Capobianchi, M R, additional

Published
2016
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18. Risk factors for type 2 diabetes in women attending menopause clinics in Italy: a cross-sectional study

Author

Di Donato, P, Giulini, N. A, Bacchi Modena, A, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gualdi, F, Golinelli, S, Bergamini, E, Masellis, G, Rastelli, S, Gigli, C, Elia, A, Marchesoni, D, Sticotti, F, Del Frate, G, Zompicchiatti, C, Marino, L, Costa, M. R, Pinto, P, Dodero, D, Storace, A, Spinelli, G, Quaranta, S, Bossi, C. M, Ollago, A, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Gambarino, G, De Pasquale, B, Polizzotti, G, Borsellino, G, Alpinelli, P, Natale, N, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Vinci, G. W, Samaja, B. A, Pasinetti, E, Penotti, M, Ognissanti, F, Pesando, P, Malanetto, C, Gallo, M, Dolfin, G, Tartaglino, P, Mossotto, D, Pistoni, A, Tarani, A, Rattazzi, P. D, Rossaro, D, Campanella, M, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Candiotto, E, Pignalosa, R, Del Pup, L, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, Di Nisio, Q, Cappa, F, Pierangeli, I, Cordone, A, Falasca, L, Ferrante, D, Serra, G. B, Cirese, E, Todaro, P. A, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Bellardini, P, Favale, W, Monti, V, Bonomo, A, Boninfante, C. E, Pietrobattista, P, Massacesi, L, Donini, G, Del Savio, F, Palombi, L, Procaccioli, P, Romani, A, Romagnoli, G, Genazzani, A. R, Gambacciani, M, Scarselli, G, Curiel, P, De Leo, V, Melani, A, Levi D'Ancona, V, Giarrè, G, Di Gioia, E, Ceccarelli, P, Massi, G. B, Cosci, S, Gacci, G, Cascianini, A, Donati Sarti, C, Bircolotti, S, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, Marongiu, G, Lai, G. R, Lai, R, Dessole, S, D'Andrea, S. A, Coppola, Null, Chiantera, A, De Placido, Null, Arienzo, R, Pastore, A. R, Tamburrino, A, Cardone, A, Izzo, S, Tesauro, R, Pascarella, A, De Silvio, M. G, Di Prisco, L, Lauda, N, Sirimarco, F, Agrimi, C, Casarella, G, Senatore, G, Ronzini, S, Ruccia, G, De Carlo, G, Pisaturo, G, Carlomagno, F, Fasolino, A, Fiorillo, F, Sorrentino, R, Ercolano, V. B, Panariello, S, Brun, A, Tropea, P, Stigliano, C. M, Amoroso, A, Vadalà, P, Coco, A, Galati, G, Barese, G, Masciari, G, Pirillo, P, Gioffrè, T, Mastrantonio, P, Cardamone, A, D'Angelo, N, Valentino, G, Barretta, R, Ferraro, G, Ferruccio, C, Agostinelli, D, Corrado, G, Scopelliti, A, Schonauer, S, Trojano, V, Bongiovanni, F, Tinelli, F, Poddi, E. R, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, E. B, D'Amore, A, Di Masi, M, Liguori, R, Dimaggio, A, Laneve, M. R, Maolo, M. C, Gravina, G, Nacci, G, Nocera, F, Lupo, A, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, F, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Cincotta, L, D'Asta, S, Bono, A, Li Calsi, L, Cavallaro Nigro, S, Schilirò, S, Repici, A, Gullo, D, Orlando, A, Specchiale, F, Papotto, A, Abruzzo, Null, Basilicata, Null, Calabria, Null, Campania, Null, Emilia, Null, Romagna, Null, Giulia, Friuli Venezia, Lazio, Null, Liguria, Null, Lombardia, Null, Marche, Null, Molise, Null, Piemonte, Null, Puglia, Null, Sardegna, Null, Sicilia, Null, Toscana, Null, Adige, Trentino Alto, Umbria, Null, D'Aosta, Valle, Veneto, Null, Massacesi, A, De Aloysio, P, Campagnoli, C, Gambacciani, A, Graziottin, A, Baldi, C, Colacurci, N, Corrado Tonti, G, Parazzini, F, Chatenoud, L., COLACURCI, Nicola, Di Donato, P, Giulini, N. A, Bacchi Modena, A, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gualdi, F, Golinelli, S, Bergamini, E, Masellis, G, Rastelli, S, Gigli, C, Elia, A, Marchesoni, D, Sticotti, F, Del Frate, G, Zompicchiatti, C, Marino, L, Costa, M. R, Pinto, P, Dodero, D, Storace, A, Spinelli, G, Quaranta, S, Bossi, C. M, Ollago, A, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Gambarino, G, De Pasquale, B, Polizzotti, G, Borsellino, G, Alpinelli, P, Natale, N, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Vinci, G. W, Samaja, B. A, Pasinetti, E, Penotti, M, Ognissanti, F, Pesando, P, Malanetto, C, Gallo, M, Dolfin, G, Tartaglino, P, Mossotto, D, Pistoni, A, Tarani, A, Rattazzi, P. D, Rossaro, D, Campanella, M, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Candiotto, E, Pignalosa, R, Del Pup, L, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, Di Nisio, Q, Cappa, F, Pierangeli, I, Cordone, A, Falasca, L, Ferrante, D, Serra, G. B, Cirese, E, Todaro, P. A, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Bellardini, P, Favale, W, Monti, V, Bonomo, A, Boninfante, C. E, Pietrobattista, P, Massacesi, L, Donini, G, Del Savio, F, Palombi, L, Procaccioli, P, Romani, A, Romagnoli, G, Genazzani, A. R, Gambacciani, M, Scarselli, G, Curiel, P, De Leo, V, Melani, A, Levi D'Ancona, V, Giarrè, G, Di Gioia, E, Ceccarelli, P, Massi, G. B, Cosci, S, Gacci, G, Cascianini, A, Donati Sarti, C, Bircolotti, S, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, Marongiu, G, Lai, G. R, Lai, R, Dessole, S, D'Andrea, S. A, Coppola, Null, Chiantera, A, De Placido, Null, Arienzo, R, Pastore, A. R, Tamburrino, A, Cardone, A, Colacurci, Nicola, Izzo, S, Tesauro, R, Pascarella, A, De Silvio, M. G, Di Prisco, L, Lauda, N, Sirimarco, F, Agrimi, C, Casarella, G, Senatore, G, Ronzini, S, Ruccia, G, De Carlo, G, Pisaturo, G, Carlomagno, F, Fasolino, A, Fiorillo, F, Sorrentino, R, Ercolano, V. B, Panariello, S, Brun, A, Tropea, P, Stigliano, C. M, Amoroso, A, Vadalà, P, Coco, A, Galati, G, Barese, G, Masciari, G, Pirillo, P, Gioffrè, T, Mastrantonio, P, Cardamone, A, D'Angelo, N, Valentino, G, Barretta, R, Ferraro, G, Ferruccio, C, Agostinelli, D, Corrado, G, Scopelliti, A, Schonauer, S, Trojano, V, Bongiovanni, F, Tinelli, F, Poddi, E. R, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, E. B, D'Amore, A, Di Masi, M, Liguori, R, Dimaggio, A, Laneve, M. R, Maolo, M. C, Gravina, G, Nacci, G, Nocera, F, Lupo, A, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, F, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Cincotta, L, D'Asta, S, Bono, A, Li Calsi, L, Cavallaro Nigro, S, Schilirò, S, Repici, A, Gullo, D, Orlando, A, Specchiale, F, Papotto, A, Abruzzo, Null, Basilicata, Null, Calabria, Null, Campania, Null, Emilia, Null, Romagna, Null, Giulia, Friuli Venezia, Lazio, Null, Liguria, Null, Lombardia, Null, Marche, Null, Molise, Null, Piemonte, Null, Puglia, Null, Sardegna, Null, Sicilia, Null, Toscana, Null, Adige, Trentino Alto, Umbria, Null, D'Aosta, Valle, Veneto, Null, Massacesi, A, De Aloysio, P, Campagnoli, C, Gambacciani, A, Graziottin, A, Baldi, C, Colacurci, N, Corrado Tonti, G, Parazzini, F, and Chatenoud, L.

Subjects
medicine.medical_specialty, Cross-sectional study, Hormone Replacement Therapy, medicine.medical_treatment, Type 2 diabetes, Motor Activity, Ambulatory Care Facilities, Age Distribution, Risk Factors, Diabetes mellitus, medicine, Odds Ratio, Humans, Obesity, Multivariate Analysi, menopausal status, Gynecology, Cross-Sectional Studie, diabetes, business.industry, Obstetrics, Risk Factor, Obstetrics and Gynecology, Hormone replacement therapy (menopause), General Medicine, Odds ratio, Middle Aged, medicine.disease, Educational Statu, Confidence interval, Menopause, Ambulatory Care Facilitie, women, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Italy, Multivariate Analysis, Educational Status, Female, business, Human
Abstract

To analyze risk factors for type 2 diabetes among women attending menopause clinics in Italy for counselling about the menopause.Women attending a network of first-level outpatient menopause clinics in Italy for general counselling about menopause or treatment of menopausal symptoms.Cross-sectional study with no exclusion criteria. Type 2 diabetes was defined according to National Diabetes Data Groups Indications and the fasting blood glucose at an oral glucose tolerance test within the previous year.Out of the 44 694 considered in this analysis, 808 had a diagnosis of diabetes type 2 (1.8%). In comparison with women aged50 years, the multivariate odds ratios (OR) of type 2 diabetes were 1.31 (95% confidence interval (CI), 0.99-1.74) for women aged 50-52 years, 1.66 (95% CI, 1.27-2.17) at 53-56 years and 2.84 (95% CI, 2.20-3.67) in women agedor = 57 years. Type 2 diabetes was less frequently reported in more educated women (OR high school/university vs. primary school = 0.44 (95% CI, 0.36-0.55)). Being overweight was associated with an increased risk of type 2 diabetes. In comparison with women reporting a low level of physical activity, the multivariate OR of type 2 diabetes was 0.67 (95% CI, 0.54-0.84) for women reporting regular physical activity. In comparison with premenopausal women, the multivariate OR of type 2 diabetes was 1.38 (95% CI, 1.03-1.84) in women with natural menopause. This finding was present also after allowing for the potential confounding effect of age. The multivariate OR of diabetes for users of hormonal replacement therapy was 0.58 (95% CI, 0.46-0.73).This large cross-sectional study suggests that postmenopausal women are at higher risk of type 2 diabetes after allowance for the effect of age. Other main determinants of risk of type 2 diabetes in women around menopause were low socioeconomic status and being overweight. Diabetes was found less frequently in those taking hormone replacement therapy.

Published
2005

19. Factors associated with climacteric symptoms in women around menopause attending menopause clinics in Italy

Author

Parazzini, Fabio, Di Donato, P., Giulini, N. A., Bacchi Modena, A., Cicchetti, G., Comitini, G., Gentile, G., Cristiani, P., Careccia, A., Esposito, E., Gualdi, F., Golinelli, S., Bergamini, E., Masellis, G., Rastelli, S., Gigli, C., Elia, A., Marchesoni, D., Sticotti, F., Del Frate, G., Zompicchiatti, C., Marino, L., Costa, M. R., Pinto, P., Dodero, D., Storace, A., Spinelli, G., Quaranta, S., Bossi, C. M., Ollago, A., Omodei, U., Vaccari, M., Luerti, M., Repetti, F., Zandonini, G., Raspagliesi, F., Dolci, F., Gambarino, G., De Pasquale, B., Polizzotti, G., Borsellino, G., Alpinelli, P., Natale, N., Colombo, D., Belloni, C., Viani, A., Cecchini, G., Vinci, G. W., Samaja, B. A., Pasinetti, E., Penotti, M., Ognissanti, F., Pesando, P., Malanetto, C., Gallo, M., Dolfin, G., Tartaglino, P., Mossotto, D., Pistoni, A., Tarani, A., Rattazzi, P. D., Rossaro, D., Campanella, M., Arisi, E., Gamper, M., Salvatores, D., Bocchin, E., Stellin, G., Meli, G., Azzini, V., Tirozzi, F., Buoso, G., Fraioli, R., Marsoni, V., Cetera, C., Sposetti, R., Candiotto, E., Pignalosa, R., Del Pup, L., Bellati, U., Angeloni, C., Buonerba, M., Garzarelli, S., Santilli, C., Mucci, M., Di Nisio, Q., Cappa, F., Pierangeli, I., Cordone, A., Falasca, L., Ferrante, D., Cirese, E., Todaro, P. A., Spagnuolo, L., Lanzone, A., Donadio, C., Fabiani, M., Baldaccini, E., Votano, S., Bellardini, P., Favale, W., Monti, V., Bonomo, A., Boninfante, C. E., Pietrobattista, P., Massacesi, L., Donini, G., Del Savio, F., Palombi, L., Procaccioli, P., Romani, A., Romagnoli, G., Genazzani, A. R., Gambacciani, M., Scarselli, G., Curiel, P., De Leo, V., Melani, A., Levi D'Ancona, V., Giarrè, G., Di Gioia, E., Ceccarelli, P., Massi, G. B., Cosci, S., Gacci, G., Cascianini, A., Donati Sarti, C., Bircolotti, S., Pupita, P., Mincigrucci, M., Spadafora, A., Santeufemia, G., Marongiu, G., Lai, G. R., Lai, R., Dessole, S., D'Andrea, S. A., Coppola, Null, Chiantera, A., De Placido, Null, Arienzo, R., Pastore, A. R., Tamburrino, A., Cardone, A., Izzo, S., Tesauro, R., Pascarella, A., De Silvio, M. G., Di Prisco, L., Lauda, N., Sirimarco, F., Agrimi, C., Casarella, G., Senatore, G., Ronzini, S., Ruccia, G., De Carlo, G., Pisaturo, G., Carlomagno, F., Fasolino, A., Fiorillo, F., Sorrentino, R., Ercolano, V. B., Panariello, S., Brun, A., Tropea, P., Stigliano, C. M., Amoroso, A., Vadalà, P., Coco, A., Galati, G., Barese, G., Masciari, G., Pirillo, P., Gioffrè, T., Mastrantonio, P., Cardamone, A., D'Angelo, N., Valentino, G., Barretta, R., Ferraro, G., Ferruccio, C., Agostinelli, D., Corrado, G., Scopelliti, A., Schonauer, S., Trojano, V., Bongiovanni, F., Tinelli, F., Poddi, E. R., Scarpello, F., Colonna, L., Fischetti, G., Doria, R., Trombetta, G., Cocca, E. B., D'Amore, A., Di Mas, M., Liguori, R., Dimaggio, A., Laneve, M. R., Maolo, M. C., Gravina, G., Nacci, G., Nocera, F., Lupo, A., Giannola, C., Graziano, R., Mezzatesta, M., Vegna, G., Giannone, G., Palumbo, G., Cancellieri, F., Mondo, A., Cordopatri, A., Carrubba, M., Mazzola, V., Cincotta, L., D'Asta, S., Bono, A., Li Calsi, L., Cavallaro Nigro, S., Schilirò, S., Repici, A., Gullo, D., Orlando, A., Specchial, F., Papotto, A., Abruzzo, Angeloni, Basilicata, D'Andrea, Calabria, Stigliano, Campania, Arienzo, Emilia, Di Donato, Romagna, Giulini, Friuli Venezia Giulia, Gigli, Lazio, Todaro, Liguria, Marino, Lombardia, Luerti, Marche, Donini, Molise, Ferrante, Piemonte, Dolfin, Puglia, Poddi, Sardegna, Santeufemia, Sicilia, Nocera, Toscana, Melani, Trentino Alto Adige, Arisi, Umbria, Mincigrucci, Valle D'Aosta, Salvatores, Veneto, Bocchin, Massacesi, A., De Aloysio, P., Campagnoli, C., Gambacciani, A., Graziottin, A., Baldi, C., Colacurci, N., Corrado Tonti, G., Chatenoud, L., COLACURCI, Nicola, Parazzini, Fabio, Di Donato, P., Giulini, N. A., Bacchi Modena, A., Cicchetti, G., Comitini, G., Gentile, G., Cristiani, P., Careccia, A., Esposito, E., Gualdi, F., Golinelli, S., Bergamini, E., Masellis, G., Rastelli, S., Gigli, C., Elia, A., Marchesoni, D., Sticotti, F., Del Frate, G., Zompicchiatti, C., Marino, L., Costa, M. R., Pinto, P., Dodero, D., Storace, A., Spinelli, G., Quaranta, S., Bossi, C. M., Ollago, A., Omodei, U., Vaccari, M., Luerti, M., Repetti, F., Zandonini, G., Raspagliesi, F., Dolci, F., Gambarino, G., De Pasquale, B., Polizzotti, G., Borsellino, G., Alpinelli, P., Natale, N., Colombo, D., Belloni, C., Viani, A., Cecchini, G., Vinci, G. W., Samaja, B. A., Pasinetti, E., Penotti, M., Ognissanti, F., Pesando, P., Malanetto, C., Gallo, M., Dolfin, G., Tartaglino, P., Mossotto, D., Pistoni, A., Tarani, A., Rattazzi, P. D., Rossaro, D., Campanella, M., Arisi, E., Gamper, M., Salvatores, D., Bocchin, E., Stellin, G., Meli, G., Azzini, V., Tirozzi, F., Buoso, G., Fraioli, R., Marsoni, V., Cetera, C., Sposetti, R., Candiotto, E., Pignalosa, R., Del Pup, L., Bellati, U., Angeloni, C., Buonerba, M., Garzarelli, S., Santilli, C., Mucci, M., Di Nisio, Q., Cappa, F., Pierangeli, I., Cordone, A., Falasca, L., Ferrante, D., Cirese, E., Todaro, P. A., Spagnuolo, L., Lanzone, A., Donadio, C., Fabiani, M., Baldaccini, E., Votano, S., Bellardini, P., Favale, W., Monti, V., Bonomo, A., Boninfante, C. E., Pietrobattista, P., Massacesi, L., Donini, G., Del Savio, F., Palombi, L., Procaccioli, P., Romani, A., Romagnoli, G., Genazzani, A. R., Gambacciani, M., Scarselli, G., Curiel, P., De Leo, V., Melani, A., Levi D'Ancona, V., Giarrè, G., Di Gioia, E., Ceccarelli, P., Massi, G. B., Cosci, S., Gacci, G., Cascianini, A., Donati Sarti, C., Bircolotti, S., Pupita, P., Mincigrucci, M., Spadafora, A., Santeufemia, G., Marongiu, G., Lai, G. R., Lai, R., Dessole, S., D'Andrea, S. A., Coppola, Null, Chiantera, A., De Placido, Null, Arienzo, R., Pastore, A. R., Tamburrino, A., Cardone, A., Colacurci, Nicola, Izzo, S., Tesauro, R., Pascarella, A., De Silvio, M. G., Di Prisco, L., Lauda, N., Sirimarco, F., Agrimi, C., Casarella, G., Senatore, G., Ronzini, S., Ruccia, G., De Carlo, G., Pisaturo, G., Carlomagno, F., Fasolino, A., Fiorillo, F., Sorrentino, R., Ercolano, V. B., Panariello, S., Brun, A., Tropea, P., Stigliano, C. M., Amoroso, A., Vadalà, P., Coco, A., Galati, G., Barese, G., Masciari, G., Pirillo, P., Gioffrè, T., Mastrantonio, P., Cardamone, A., D'Angelo, N., Valentino, G., Barretta, R., Ferraro, G., Ferruccio, C., Agostinelli, D., Corrado, G., Scopelliti, A., Schonauer, S., Trojano, V., Bongiovanni, F., Tinelli, F., Poddi, E. R., Scarpello, F., Colonna, L., Fischetti, G., Doria, R., Trombetta, G., Cocca, E. B., D'Amore, A., Di Mas, M., Liguori, R., Dimaggio, A., Laneve, M. R., Maolo, M. C., Gravina, G., Nacci, G., Nocera, F., Lupo, A., Giannola, C., Graziano, R., Mezzatesta, M., Vegna, G., Giannone, G., Palumbo, G., Cancellieri, F., Mondo, A., Cordopatri, A., Carrubba, M., Mazzola, V., Cincotta, L., D'Asta, S., Bono, A., Li Calsi, L., Cavallaro Nigro, S., Schilirò, S., Repici, A., Gullo, D., Orlando, A., Specchial, F., Papotto, A., Abruzzo, Angeloni, Basilicata, D'Andrea, Calabria, Stigliano, Campania, Arienzo, Emilia, Di Donato, Romagna, Giulini, Friuli Venezia Giulia, Gigli, Lazio, Todaro, Liguria, Marino, Lombardia, Luerti, Marche, Donini, Molise, Ferrante, Piemonte, Dolfin, Puglia, Poddi, Sardegna, Santeufemia, Sicilia, Nocera, Toscana, Melani, Trentino Alto Adige, Arisi, Umbria, Mincigrucci, Valle D'Aosta, Salvatore, Veneto, Bocchin, Massacesi, A., De Aloysio, P., Campagnoli, C., Gambacciani, A., Graziottin, A., Baldi, C., Colacurci, N., Corrado Tonti, G., and Chatenoud, L.

Subjects
Aging, Epidemiology, Cross-sectional study, Marital Statu, Ambulatory Care Facilities, Body Mass Index, Age Factor, Reproductive History, Depression (differential diagnoses), Depression, Smoking, Age Factors, Headache, Obstetrics and Gynecology, Middle Aged, Menopause, Italy, Educational Status, Female, medicine.symptom, Climacteric, Human, Adult, medicine.medical_specialty, Hot Flashe, Logistic Model, Symptom, Irritability, General Biochemistry, Genetics and Molecular Biology, medicine, Humans, Aged, Cross-Sectional Studie, Marital Status, business.industry, Odds ratio, medicine.disease, Educational Statu, Diet, Ambulatory Care Facilitie, Cross-Sectional Studies, Logistic Models, Risk factors, Hot Flashes, Symptoms, Physical therapy, Risk factor, business, Body mass index, Demography
Abstract

OBJECTIVE To obtain data on correlates of climacteric symptoms in women around menopause attending menopause clinics in Italy. METHODS Since 1997 a large cross sectional study has been conducted on the characteristics of women around menopause attending a network of first level menopause outpatient's clinics in Italy. A total of 66,501 (mean age 54.4 years) women are considered in the present paper. RESULTS The odds ratios of moderate and severe hot flashes/night sweats were lower in more educated women and (for severe symptoms only) in women reporting regular physical activity. Depression, difficulty to sleep, forgetfulness and irritability tended to be less frequent in more educated women and (depression only) in women reporting regular physical activity. Parous women reported more frequently these symptoms. CONCLUSIONS This large study confirms in Southern European population that low education, body mass index and low physical activity are associated with climacteric symptoms. Parous women are at greater risk of psychological symptoms.

Published
2005

21. LATTE CRUDO RISCHI E OPPORTUNITA’

Author

Tremonte, P., Pannella, G., Falasca, L., Tipaldi, L., Succi, Mariantonietta, Capilongo, V., R. Coppola V., and Sorrentino, Elena

Published
2012

28. More than two sides of a coin? How to detect the multiple activities of type 2 transglutaminase

Author

Rodolfo, C, Falasca, L, Di Giacomo, G, Mastroberardino, P, and Piacentini, M

Subjects
Mice, Knockout, Mice, Settore BIO/06, Transglutaminases, Phagocytosis, Knockout, Fluorescent Antibody Technique, Apoptosis, Immunohistochemistry, Animals, Humans, Biotinylation
Abstract

Programmed cell death (PCD) by apoptosis has been widely characterized as a process in which the expression and protein activation of a gene must be regulated in a very precise way in order to achieve the elimination of the dying cell without disturbing the neighborhoods. One of the first genes observed to be induced during the onset of PCD is the one coding for type 2 transglutaminase (TG2). Since the late 1990s, the unveiling of different new properties and enzymatic activities suggested the involvement of TG2 in a variety of cellular processes other than PCD and rendered the study of this protein more and more complicated.

Published
2008

39. Determinants of body mass index in women around menopause attending menopause clinics in Italy

Author

DE ALOYSIO, D, DI DONATO, P, Giulini, Na, Modena, Ab, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gualdi, F, Golinelli, S, Bergamini, E, Masellis, G, Rastelli, S, Gigli, C, Elia, A, Marchesoni, D, Sticotti, F, DEL FRATE, G, Zompicchiatti, C, Marino, L, Costa, Mr, Pinto, P, Dodero, D, Storace, A, Spinelli, G, Quaranta, S, Bossi, Cm, Ollago, A, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Gambarino, G, DE PASQUALE, B, Polizzotti, G, Borsellino, G, Alpinelli, P, Natale, N, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Vinci, Gw, Samaja, Ba, Pasinetti, E, Penotti, M, Ognissanti, F, Pesando, P, Malanetto, C, Gallo, M, Dolfin, G, Tartaglino, P, Mossotto, D, Pistoni, A, Tarani, A, Rattazzi, Pd, Rossaro, D, Campanella, M, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Candiotto, E, Pignalosa, R, DEL PUP, L, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, DI NISIO, Q, Cappa, F, Pierangeli, I, Cordone, A, Falasca, L, Ferrante, D, Cirese, E, Todaro, Pa, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Bellardini, P, Favale, W, Monti, V, Bonomo, A, Boninfante, Ce, Pietrobattista, P, Massacesi, L, Donini, G, DEL SAVIO, F, Palombi, L, Procaccioli, P, Romani, A, Romagnoli, G, Genazzani, Ar, Gambacciani, M, Scarselli, G, Curiel, P, DE LEO, V, Melani, A, D'Ancona, Vl, Giarre, G, DI GIOIA, E, Ceccarelli, P, Massi, Gb, Cosci, S, Gacci, G, Cascianini, A, Sarti, Cd, Bircolotti, S, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, Marongiu, G, Lai, Gr, Lai, R, Dessole, S, D'Andrea, Sa, Coppola, Chiantera, A, Placido, De, Arienzo, R, Pastore, Ar, Tamburrino, A, Cardone, A, Colacurci, N, Izzo, S, Tesauro, R, Pascarella, A, DE SILVIO MG, DI PRISCO, L, Lauda, N, Sirimarco, F, Agrimi, C, Casarella, G, Senatore, G, Ronzini, S, Ruccia, G, DE CARLO, G, Pisaturo, G, Carlomagno, F, Fasolino, A, Fiorillo, F, Sorrentino, R, Ercolano, Vb, Panariello, S, Brun, A, Tropea, P, Stigliano, Cm, Amoroso, A, Vadala, P, Coco, A, Galati, G, Barese, G, Masciari, G, Pirillo, P, Gioffre, T, Mastrantonio, P, Cardamone, A, D'Angelo, N, Valentino, G, Barretta, R, Ferraro, G, Ferruccio, C, Agostinelli, D, Corrado, G, Scopelliti, A, Schonauer, S, Trojano, V, Bongiovanni, F, Tinelli, F, Poddi, Er, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, Eb, D'Amore, A, DI MASI, M, Liguori, R, Dimaggio, A, Laneve, Mr, Maolo, Mc, Gravina, G, Nacci, G, Nocera, F, Lupo, A, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, Francesco, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, D'Asta, S, Bono, A, Calsi, Ll, Nigro, Sc, Schiliro, S, Repici, A, Gullo, D, Orlando, A, Specchiale, F, Papotto, A, Massacesi, A, DE ALOYSIO, P, Campagnoli, C, Gambacciani, A, Graziottin, A, Baldi, C, Parazzini, F, Chatenoud, L, and Chiaffarino, F.

Published
2003

40. Premature ovarian failure: frequency and risk factors among women attending a network of menopause clinics in Italy

Author

DE ALOYSIO, D, DI DONATO, P, Giulini, Na, Modena, Ab, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gualdi, F, Golinelli, S, Bergamini, E, Masellis, G, Rastelli, S, Gigli, C, Elia, A, Marchesoni, D, Sticotti, F, DEL FRATE, G, Zompicchiatti, C, Marino, L, Costa, Mr, Pinto, P, Dodero, D, Storace, A, Spinelli, G, Quaranta, S, Bossi, Cm, Ollago, A, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Gambarino, G, DE PASQUALE, B, Polizzotti, G, Borsellino, G, Alpinelli, P, Natale, N, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Vinci, Gw, Samaja, Ba, Pasinetti, E, Penotti, M, Ognissanti, F, Pesando, P, Malanetto, C, Gallo, M, Dolfin, G, Tartaglino, P, Mossotto, D, Pistoni, A, Tarani, A, Rattazzi, Pd, Rossaro, D, Campanella, M, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Candiotto, E, Pignalosa, R, DEL PUP, L, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, DI NISIO, Q, Cappa, F, Pierangeli, I, Cordone, A, Falasca, L, Ferrante, D, Serra, Gb, Cirese, E, Todaro, Pa, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Bellardini, P, Favale, W, Monti, V, Bonomo, A, Boninfante, Ce, Pietrobattista, P, Massacesi, L, Donini, G, DEL SAVIO, F, Palombi, L, Procaccioli, P, Romani, A, Romagnoli, G, Genazzani, Ar, Gambacciani, M, Scarselli, G, Curiel, P, DE LEO, V, Melani, A, D'Ancona, Vl, Lorenzo, Bs, Giarre, G, DI GIOIA, E, Ceccarelli, P, Massi, Gb, Cosci, S, Gacci, G, Cascianini, A, Sarti, Cd, Bircolotti, S, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, Marongiu, G, Lai, Gr, Lai, R, Dessole, S, D'Andrea, Sa, Coppola, Chiantera, A, Placido, D, Arienzo, R, Pastore, Ar, Tamburrino, A, Cardone, A, Colacurci, N, Izzo, S, Tesauro, R, Pascarella, A, DE SILVIO MG, DI PRISCO, L, Lauda, N, Sirimarco, F, Agrimi, C, Casarella, G, Senatore, G, Ronzini, S, Ruccia, G, DE CARLO, G, Pisaturo, G, Carlomagno, F, Fasolino, A, Fiorillo, F, Sorrentino, R, Ercolano, Vb, Panariello, S, Brun, A, Tropea, P, Stigliano, Cm, Amoroso, A, Vadala, P, Coco, A, Galati, G, Barese, G, Masciari, G, Pirillo, P, Mastrantonio, P, Cardamone, A, D'Angelo, N, Valentino, G, Barretta, R, Ferraro, G, Ferruccio, C, Agostinelli, D, Corrado, G, Scopelliti, A, Schonauer, S, Trojano, V, Bongiovanni, F, Tinelli, F, Poddi, Er, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, Eb, D'Amore, A, DI MASI, M, Liguori, R, Dimaggio, A, Laneve, Mr, Maolo, Mc, Gravina, G, Nacci, G, Nocera, F, Lupo, A, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, Francesco, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Cincotta, L, D'Asta, S, Bono, A, Calsi, Ll, Nigro, Sc, Schiliro, S, Repici, A, Gullo, D, Orlando, A, Specchiale, F, and Papotto, A.

Published
2003

41. Transglutaminase overexpression sensitizes neuronal cell lines to apoptosis by increasing mitochondrial membrane potential and cellular oxidative stress

Author

Piacentini, M, Farrace, Mg, Piredda, L, Matarrese, P, Ciccosanti, F, Falasca, L, Rodolfo, C, Giammarioli, A, Verderio, E, Griffin, M, and Malorni, W

Subjects
Settore BIO/06, Gene Expression, Apoptosis, Transfection, Mitochondrial Membrane Transport Proteins, Ion Channels, Membrane Potentials, Neuroblastoma, Mice, GTP-Binding Proteins, Animals, Humans, Enzyme Inhibitors, 3T3 Cells, Oxidative Stress, Cyclosporine, Tetracycline, Mitochondria, Intracellular Membranes, Flow Cytometry, Transglutaminases, Tumor Cells, Cultured, Neurons, Child, Oxidation-Reduction, Cultured, Tumor Cells
Published
2002

42. Risk of low bone density in women attending menopause clinics in Italy

Author

DE ALOYSIO, D, DI DONATO, P, Giulini, Na, Modena, B, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gigli, C, Mandruzzato, G, Petraglia, F, Bertelli, F, DEL FRATE, G, Garofalo, S, Marino, L, Costa, Mr, Quaranta, S, Bossi, Cm, Amantea, P, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Zacchi, V, Bennici, S, Polizzotti, G, Bottino, S, Raffaelli, F, Minervini, C, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Winkler, S, Samaja, Ba, Pasinetti, E, Penotti, M, Malanetto, C, Massobrio, M, Campagnoli, C, Dolfin, G, Tartaglino, P, Mossotto, D, Pesando, P, Pacilli, L, Rattazzi, Pd, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, DI NISIO, Q, Falasca, L, Ferrante, D, Cirese, E, Todaro, Pa, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Serra, Gb, Bellardini, P, Massacesi, L, Donini, G, Genazzani, Ra, Scarselli, G, Curiel, P, DE LEO, V, Melani, A, D'Ancona, Vl, Giarre, G, DI GIOIA, E, Ciccarelli, P, Sarti, Cd, Balsotti, G, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, D'Andrea, A, Chiantera, A, Montemagno, U, Staiano, S, Arienzo, R, Pastore, Ar, Tamburrino, A, Colacurci, A, Izzo, S, Zumpano, N, Pascarella, A, DE SILVIO MG, DI PRISCO, L, Lauda, N, Sorrentino, O, Agrimi, C, Casarella, G, Pisaturo, G, Senatore, G, Ruccia, G, Fasolino, A, Tropea, P, Stigliano, Cm, Giannice, C, Vadala, P, Coco, A, Lombardo, R, Barese, G, Masciari, G, Pirillo, P, Gioffre, T, Ferruccio, C, Agostinelli, D, Tonti, Gc, Scopelliti, A, Schonauer, S, Bongiovanni, F, Tinelli, F, Poddi, Er, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, Eb, Carone, D, Nocera, F, Giambanco, V, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, Francesco, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Ettore, G, D'Asta, S, DI LIBERTO, P, Massacesi, A, DE ALOYSIO, P, Ognissanti, F, Gambacciani, A, Graziottin, A, Baldi, C, Colacurci, N, and Parazzini, F.

Published
2002

44. General and medical factors associated with hormone replacement therapy among women attending menopause clinics in Italy

Author

DE ALOYSIO, D, DI DONATO, P, Giulini, Na, Modena, B, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gigli, C, Mandruzzato, G, Petraglia, F, Bertelli, F, DEL FRATE, G, Garofalo, S, Marino, L, Costa, Mr, Quaranta, S, Bossi, Cm, Amantea, P, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Zacchi, V, Bennici, S, Polizzotti, G, Bottino, S, Raffaelli, F, Minervini, C, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Winkler, S, Samaja, Ba, Pasinetti, E, Penotti, M, Malanetto, C, Massobrio, M, Campagnoli, C, Dolfin, G, Tartaglino, P, Mossotto, D, Pesando, P, Pacilli, L, Rattazzi, Pd, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, DI NISIO, Q, Falasca, L, Ferrante, D, Cirese, E, Todaro, Pa, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Serra, Gb, Bellardini, P, Massacesi, L, Donini, G, Genazzani, Ra, Scarselli, G, Curiel, P, DE LEO, V, Melani, A, D'Ancona, Vl, Giarre, G, DI GIOIA, E, Ciccarelli, P, Sarti, Cd, Balsotti, G, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, D'Andrea, A, Chiantera, A, Montemagno, U, Staiano, S, Arienzo, R, Pastore, Ar, Tamburrino, A, Colacurci, A, Izzo, S, Zumpano, N, Pascarella, A, DE SILVIO MG, DI PRISCO, L, Lauda, N, Sorrentino, O, Agrimi, C, Casarella, G, Pisaturo, G, Senatore, G, Ruccia, G, Fasolino, A, Tropea, P, Stigliano, Cm, Giannice, C, Vadala, P, Coco, A, Lombardo, R, Barese, G, Masciari, G, Pirillo, P, Gioffre, T, Ferruccio, C, Agostinelli, D, Tonti, Gc, Scopelliti, A, Schonauer, S, Bongiovanni, F, Tinelli, F, Poddi, Er, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, Eb, Carone, D, Nocera, F, Giambanco, V, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, Francesco, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Ettore, G, D'Asta, S, DI LIBERTO, P, Massacesi, A, DE ALOYSIO, P, Ognissanti, F, Gambacciani, A, Graziottin, A, Baldi, C, Colacurci, N, Parazzini, F, Chatenoud, L, and Chiaffarino, F.

Published
2001

47. ESX-1 dependent impairment of autophagic flux by Mycobacterium tuberculosis in human dendritic cells

Author

Romagnoli, Angela, Etna, Mp, Giacomini, E, Pardini, M, Remoli, Me, Corazzari, M, Falasca, L, Goletti, D, Gafa, V, Simeone, R, Delogu, Giovanni, Piacentini, M, Brosch, R, Fimia, Gm, Coccia, Em, Delogu, Giovanni (ORCID:0000-0003-0182-8267), Romagnoli, Angela, Etna, Mp, Giacomini, E, Pardini, M, Remoli, Me, Corazzari, M, Falasca, L, Goletti, D, Gafa, V, Simeone, R, Delogu, Giovanni, Piacentini, M, Brosch, R, Fimia, Gm, Coccia, Em, and Delogu, Giovanni (ORCID:0000-0003-0182-8267)

Abstract

Emerging evidence points to an important role of autophagy in the immune response mediated by dendritic cells (DC) against Mycobacterium tuberculosis (Mtb). Since current vaccination based on Bacillus Calmette-Guerin (BCG) is unable to stop the tuberculosis epidemic, a deeper comprehension of the alterations induced by Mtb in DC is essential for setting new vaccine strategies. Here, we compared the capacity of virulent (H37Rv) and avirulent (H37Ra) Mtb strains as well as BCG to modulate autophagy in human primary DC. We found that Mtb H37Rv impairs autophagy at the step of autophagosome-lysosome fusion. In contrast, neither Mtb H37Ra nor BCG strains were able to hamper autophagosome maturation. Both these attenuated strains have a functional inhibition of the 6kD early secreted antigenic target ESAT-6, an effector protein of the ESAT-6 Secretion System-1(ESX-1)/type VII secretion system. Notably, the ability to inhibit autophagy was fully restored in recombinant BCG and Mtb H37Ra strains in which ESAT-6 secretion was re-established by genetic complementation using either the ESX-1 region from Mtb (BCG::ESX-1) or the PhoP gene (Mtb H37Ra::PhoP), a regulator of ESAT-6 secretion. Importantly, the autophagic block induced by Mtb was overcome by rapamycin treatment leading to an increased interleukin-12 expression and, in turn, to an enhanced capacity to expand a Th1-oriented response. Collectively, our study demonstrated that Mtb alters the autophagic machinery through the ESX-1 system, and thereby opens new exciting perspectives to better understand the relationship between Mtb virulence and its ability to escape the DC-mediated immune response.

Published
2012

48. Determinants of hysterectomy and oophorectomy in women attending menopause clinics in Italy

Author

DE ALOYSIO, D, DI DONATO, P, Giulini, Na, Modena, B, Cicchetti, G, Comitini, G, Gentile, G, Cristiani, P, Careccia, A, Esposito, E, Gigli, C, Mandruzzato, G, Petraglia, F, Bertelli, F, DEL FRATE, G, Garofalo, S, Marino, L, Costa, Mr, Quaranta, S, Bossi, Cm, Amantea, P, Omodei, U, Vaccari, M, Luerti, M, Repetti, F, Zandonini, G, Raspagliesi, F, Dolci, F, Zacchi, V, Bennici, S, Polizzotti, G, Bottino, S, Raffaelli, F, Minervini, C, Colombo, D, Belloni, C, Viani, A, Cecchini, G, Winkler, S, Samaja, Ba, Pasinetti, E, Penotti, M, Malanetto, C, Massobrio, M, Campagnoli, C, Dolfin, G, Tartaglino, P, Mossotto, D, Pesando, P, Pacilli, L, Rattazzi, Pd, Arisi, E, Gamper, M, Salvatores, D, Bocchin, E, Stellin, G, Meli, G, Azzini, V, Tirozzi, F, Buoso, G, Fraioli, R, Marsoni, V, Cetera, C, Sposetti, R, Bellati, U, Angeloni, C, Buonerba, M, Garzarelli, S, Santilli, C, Mucci, M, DI NISIO, Q, Falasca, L, Ferrante, D, Cirese, E, Todaro, Pa, Romanini, C, Spagnuolo, L, Lanzone, A, Donadio, C, Fabiani, M, Baldaccini, E, Votano, S, Serra, Gb, Bellardini, P, Massacesi, L, Donini, G, Genazzani, Ra, Scarselli, G, Curiel, P, DE LEO, V, Melani, A, D'Ancona, Vl, Giarre, G, DI GIOIA, E, Ciccarelli, P, Sarti, Cd, Balsotti, G, Pupita, P, Mincigrucci, M, Spadafora, A, Santeufemia, G, D'Andrea, A, Chiantera, A, Montemagno, U, Staiano, S, Arienzo, R, Pastore, Ar, Tamburrino, A, Colacurci, A, Izzo, S, Zumpano, N, Pascarella, A, DE SILVIO MG, DI PRISCO, L, Lauda, N, Sorrentino, O, Agrimi, C, Casarella, G, Pisaturo, G, Senatore, G, Ruccia, G, Fasolino, A, Tropea, P, Stigliano, Cm, Giannice, C, Vadala, P, Coco, A, Lombardo, R, Barese, G, Masciari, G, Pirillo, P, Gioffre, T, Ferruccio, C, Agostinelli, D, Tonti, Gc, Scopelliti, A, Schonauer, S, Bongiovanni, F, Tinelli, F, Poddi, Er, Scarpello, F, Colonna, L, Fischetti, G, Doria, R, Trombetta, G, Cocca, Eb, Carone, D, Nocera, F, Giambanco, V, Giannola, C, Graziano, R, Mezzatesta, M, Vegna, G, Giannone, G, Palumbo, G, Cancellieri, Francesco, Mondo, A, Cordopatri, A, Carrubba, M, Mazzola, V, Ettore, G, D'Asta, S, DI LIBERTO, P, Angeloni, D'Andrea, Stigliano, Arienzo, Donato, Di, Giulini, Gigli, Ricci, Marino, Luerti, Donini, Dolfin, Poddi, Santeufemia, Nocera, Melani, Messini, Mincigrucci, Salvatores, Bocchin, Massacesi, A, DE ALOYSIO, P, Ognissanti, F, Gambacciani, A, Graziottin, A, Baldi, C, Parazzini, F, Chatenoud, L, and Chiaffarino, F.

Published
2000

50. Oncogenic B-RAF signaling in melanoma impairs the therapeutic advantage of autophagy inhibition

Author

Armstrong, J. L., Corazzari, M., Martin, S., Pagliarini, Vittoria, Falasca, L., Hill, D. S., Ellis, N., Sabah, S. A., Redfern, C. P. F., Fimia, G. M., Piacentini, M., Lovat, P. E., Pagliarini V. (ORCID:0000-0002-2388-0675), Armstrong, J. L., Corazzari, M., Martin, S., Pagliarini, Vittoria, Falasca, L., Hill, D. S., Ellis, N., Sabah, S. A., Redfern, C. P. F., Fimia, G. M., Piacentini, M., Lovat, P. E., and Pagliarini V. (ORCID:0000-0002-2388-0675)

Abstract

Purpose: Metastatic melanoma is characterized by extremely poor survival rates and hence novel therapies are urgently required. The ability of many anticancer drugs to activate autophagy, a lysosomalmediated catabolic process which usually promotes cell survival, suggests targeting the autophagy pathway may be a novel means to augment therapy. Experimental Design: Autophagy and apoptosis were assessed in vitro in human melanoma cell lines in response to clinically achievable concentrations of the endoplasmic reticulum (ER) stress-inducing drugs fenretinide or bortezomib, and in vivo using a s.c. xenograft model. Results: Autophagy was activated in response to fenretinide or bortezomib in B-RAF wild-type cells, shown by increased conversion of LC3 to the autophagic vesicle-associated form (LC3-II) and redistribution to autophagosomes and autolysosomes, increased acidic vesicular organelle formation and autophagic vacuolization. In contrast, autophagy was significantly reduced in B-RAF-mutated melanoma cells, an effect attributed partly to oncogenic B-RAF. Rapamycin treatment was unable to stimulate LC3-II accumulation or redistribution in the presence of mutated B-RAF, indicative of de-regulated mTORC1-dependent autophagy. Knockdown of Beclin-1 or ATG7 sensitized B-RAF wild-type cells to fenretinide-or bortezomib-induced cell death, demonstrating a pro-survival function of autophagy. In addition, autophagy was partially reactivated in B-RAF-mutated cells treated with the BH3 mimetic ABT737 in combination with fenretinide or bortezomib, suggesting autophagy resistance is partly mediated by abrogated Beclin-1 function. Conclusions: Our findings suggest inhibition of autophagy in combination with ER stress-inducing agents may represent a means by which to harness autophagy for the therapeutic benefit of B-RAF wild-type melanoma. © 2011 American Association for Cancer Research.

Published
2011

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