1. Evaluation of osteoblastic cell behavior upon culture on titanium substrates photo-functionalized by vacuum ultra-violet treatment.
- Author
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Nakashima Y, Akaike M, Kounoura M, Hayashi K, Morita K, Oki Y, and Nakanishi Y
- Subjects
- Cell Adhesion drug effects, Cell Adhesion radiation effects, Cell Differentiation drug effects, Cell Proliferation drug effects, Cells, Cultured, Humans, Osteoblasts drug effects, Osteoblasts radiation effects, Osteogenesis genetics, Osteogenesis radiation effects, Substrate Specificity, Surface Properties drug effects, Surface Properties radiation effects, Ultraviolet Rays adverse effects, Vacuum, Cell Differentiation radiation effects, Cell Proliferation radiation effects, Osteogenesis drug effects, Titanium pharmacology
- Abstract
Photo-functionalization of titanium orthopedic/prosthetic implants using ultraviolet illumination is known to improve osteogenesis. Therefore, in this study, we aimed to examine the influence of vacuum ultraviolet (VUV)-treated titanium surfaces on osteoblast cell adhesion, activity, and differentiation. Osteoblastic cells were cultured on titanium substrates treated with various VUV treatment conditions (0, 6.2, 18.7, and 37.4 J/cm
2 ) and their behavior was evaluated. The results revealed that cell adhesion was increased whereas cell activity and differentiation ability were decreased upon cell culture on VUV-treated substrates. In particular, cell activity and differentiation ability were dramatically suppressed with 18.7 J/cm2 VUV irradiation. Within the limitations of this cell-based experiment, we clarified the VUV treatment conditions in which cell adhesion was improved but cell activity and differentiation ability were suppressed. These results indicate that VUV-treatment can be used to influence cell growth properties and can be used to accelerate or suppress cell differentiation on implant substrates., (Copyright © 2021 Elsevier Inc. All rights reserved.)- Published
- 2022
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