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Gold cleaning methods for preparation of cell culture surfaces for self-assembled monolayers of zwitterionic oligopeptides.
- Source :
-
Journal of bioscience and bioengineering [J Biosci Bioeng] 2018 May; Vol. 125 (5), pp. 606-612. Date of Electronic Publication: 2018 Jan 17. - Publication Year :
- 2018
-
Abstract
- Self-assembled monolayers (SAMs) have been used to elucidate interactions between cells and material surface chemistry. Gold surfaces modified with oligopeptide SAMs exhibit several unique characteristics, such as cell-repulsive surfaces, micropatterns of cell adhesion and non-adhesion regions for control over cell microenvironments, and dynamic release of cells upon external stimuli under culture conditions. However, basic procedures for the preparation of oligopeptide SAMs, including appropriate cleaning methods of the gold surface before modification, have not been fully established. Because gold surfaces are readily contaminated with organic compounds in the air, cleaning methods may be critical for SAM formation. In this study, we examined the effects of four gold cleaning methods: dilute aqua regia, an ozone water, atmospheric plasma, and UV irradiation. Among the methods, UV irradiation most significantly improved the formation of oligopeptide SAMs in terms of repulsion of cells on the surfaces. We fabricated an apparatus with a UV light source, a rotation table, and HEPA filter, to treat a number of gold substrates simultaneously. Furthermore, UV-cleaned gold substrates were capable of detaching cell sheets without serious cell injury. This may potentially provide a stable and robust approach to oligopeptide SAM-based experiments for biomedical studies.<br /> (Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Coated Materials, Biocompatible chemistry
Coated Materials, Biocompatible radiation effects
Color
Equipment Reuse
Protein Multimerization
Surface Properties radiation effects
Ultraviolet Rays
Cell Adhesion radiation effects
Cell Culture Techniques instrumentation
Cell Culture Techniques methods
Detergents
Gold chemistry
Oligopeptides chemistry
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1347-4421
- Volume :
- 125
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of bioscience and bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 29352711
- Full Text :
- https://doi.org/10.1016/j.jbiosc.2017.12.014