Back to Search
Start Over
Effect of radiation-induced endothelial cell injury on platelet regeneration by megakaryocytes.
- Source :
-
Journal of radiation research [J Radiat Res] 2017 Jul 01; Vol. 58 (4), pp. 456-463. - Publication Year :
- 2017
-
Abstract
- Thrombocytopenia is an important cause of hemorrhage and death after radiation injury, but the pathogenesis of radiation-induced thrombocytopenia has not been fully characterized. Here, we investigated the influence of radiation-induced endothelial cell injury on platelet regeneration. We found that human umbilical vein endothelial cells (HUVECs) underwent a high rate of apoptosis, accompanied by a significant reduction in the expression of vascular endothelial growth factor (VEGF) at 96 h after radiation. Subsequent investigations revealed that radiation injury lowered the ability of HUVECs to attract migrating megakaryocytes (MKs). Moreover, the adhesion of MKs to HUVECs was markedly reduced when HUVECs were exposed to radiation, accompanied by a decreased production of platelets by MKs. In vivo study showed that VEGF treatment significantly promoted the migration of MKs into the vascular niche and accelerated platelet recovery in irradiated mice. Our studies demonstrate that endothelial cell injury contributes to the slow recovery of platelets after radiation, which provides a deeper insight into the pathogenesis of thrombocytopenia induced by radiation.<br /> (© The Author 2017. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.)
- Subjects :
- Animals
Apoptosis radiation effects
Cell Adhesion radiation effects
Cell Movement radiation effects
Cell Shape radiation effects
Human Umbilical Vein Endothelial Cells metabolism
Human Umbilical Vein Endothelial Cells pathology
Humans
Male
Mice, Inbred BALB C
Thrombocytopenia pathology
Vascular Endothelial Growth Factor A administration & dosage
Vascular Endothelial Growth Factor A metabolism
X-Rays
Blood Platelets radiation effects
Human Umbilical Vein Endothelial Cells radiation effects
Megakaryocytes radiation effects
Regeneration radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1349-9157
- Volume :
- 58
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of radiation research
- Publication Type :
- Academic Journal
- Accession number :
- 28402443
- Full Text :
- https://doi.org/10.1093/jrr/rrx015