1. Isoquinoline alkaloids from berberis vulgaris as potential lead compounds for the treatment of alzheimer's disease
- Author
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Vincenza Andrisano, Daniel Jun, Jan Korabecny, Jana Marikova, Daniel I. Perez, Lucie Nováková, Tomas Kucera, Lucie Cahlíková, Tomáš Siatka, Jiri Kunes, Daniela Hulcová, Lubomír Opletal, Anna Hošt'álková, Hostalkova A., Marikova J., Opletal L., Korabecny J., Hulcova D., Kunes J., Novakova L., Perez D.I., Jun D., Kucera T., Andrisano V., Siatka T., and Cahlikova L.
- Subjects
Berberis ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Plant Exudates ,Pharmaceutical Science ,Oligopeptidase ,Berbamine ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Alkaloids ,Alzheimer Disease ,Drug Discovery ,Humans ,Isoquinoline ,IC50 ,Butyrylcholinesterase ,Pharmacology ,010405 organic chemistry ,Organic Chemistry ,Isoquinoline alkaloids, lead compounds, alzheimer's disease ,Nuclear magnetic resonance spectroscopy ,Isoquinolines ,Acetylcholinesterase ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,chemistry ,Blood-Brain Barrier ,Molecular Medicine ,Cholinesterase Inhibitors ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Three new alkaloids, bersavine (3), muraricine (4), and berbostrejdine (8), together with seven known isoquinoline alkaloids (1-2, 5-7, 9, and 10) were isolated from an alkaloidal extract of the root bark of Berberis vulgaris. The structures of the isolated compounds were determined by spectroscopic methods, including 1D and 2D NMR techniques, HRMS, and optical rotation, and by comparison of the obtained data with those in the literature. The NMR data of berbamine (5), aromoline (6), and obamegine (7) were completely assigned employing 2D NMR experiments. Alkaloids isolated in sufficient amounts were evaluated for their in vitro acetylcholinesterase, butyrylcholinesterase (BuChE), prolyl oligopeptidase, and glycogen synthase kinase-3β inhibitory activities. Selected compounds were studied for their ability to permeate through the blood-brain barrier. Significant human BuChE ( hBuChE) inhibitory activity was demonstrated by 6 (IC50 = 0.82 ± 0.10 μM). The in vitro data were further supported by computational analysis that showed the accommodation of 6 in the active site of hBuChE.
- Published
- 2019