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Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice
- Source :
- Chemico-biological interactions. 275
- Publication Year :
- 2017
-
Abstract
- In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model. The results showed that haemanthamine, lycorine and haemanthidine exerted the highest antiproliferative activity. The mean growth percent (GP) value after a single-dose 10 μM treatment was for haemanthamine 21%, for lycorine 21% and for haemanthidine 27% that of untreated control cells (100%). Furthermore, haemanthamine, lycorine and haemanthidine exhibited significant cytotoxicities against all the tested cell lines with individual IC50 values in the micromolar range. Dynamic real-time measures of impedance by xCELLigence indicated that these three compounds suppress cell proliferation after 10 h of treatment at a concentration of 10 μM or higher. Regrettably, in a follow-up in vivo antitumor activity study, haemanthamine showed no statistically significant reduction in the tumor size with no prolongation of survival time of Ehrlich tumor-bearing mice. Taken together, these results provide a new clue and guidance for exploiting Amaryllidaceae alkaloids as anticancer agents.
- Subjects :
- Transplantation, Heterologous
Cell analysis
Apoptosis
Kaplan-Meier Estimate
Biology
Pharmacology
Toxicology
01 natural sciences
chemistry.chemical_compound
Mice
Alkaloids
In vivo
Cell Line, Tumor
Animals
Humans
Amaryllidaceae Alkaloids
Carcinoma, Ehrlich Tumor
Cell Proliferation
010405 organic chemistry
Cell growth
Amaryllidaceae
General Medicine
Lycorine
biology.organism_classification
Antineoplastic Agents, Phytogenic
In vitro
0104 chemical sciences
010404 medicinal & biomolecular chemistry
chemistry
Cell culture
Female
Drug Screening Assays, Antitumor
Subjects
Details
- ISSN :
- 18727786
- Volume :
- 275
- Database :
- OpenAIRE
- Journal :
- Chemico-biological interactions
- Accession number :
- edsair.doi.dedup.....0aa551fc3057128ab8b9bb84ed6fe545