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为开发亚油酸生产新资源，对筛选获得的一株丝状微藻克里藻，考察培养基氮浓度、培养基磷浓度、光强、培养温度和环境pH对其生长、氮消耗、油脂含量和油脂脂肪酸组成的影响。结果表明：培养基氮浓度对克里藻生长没有明显影响，但高氮不利于油脂和不饱和脂肪酸的合成；培养基磷浓度过低会抑制细胞生长，过高对细胞有毒害作用；适当提高光强有利于细胞生长，但对细胞内油脂含量影响不大，低光强培养有利于亚油酸等不饱和脂肪酸的合成；培养温度对克里藻生长与油脂积累影响不大，低温培养有利于亚油酸的合成；环境pH对细胞生长、油脂积累和亚油酸合成影响明显，pH 6.0~7.5的弱酸-中性条件下细胞生长最快，油脂含量更高；控制初始氮浓度3~6 mmol/L、磷浓度0.115~0.230 mmol/L、光强100~300 μmol/（m2·s)、培养温度15~30 ℃、环境pH 7.5左右，克里藻细胞可积累42%左右的油脂，油脂中亚油酸含量在65%~75%。综上，丝状微藻克里藻是一种有潜力的亚油酸生产新资源。In order to develop new resources for the production of linoleic acid, with a filamentous microalgae Klebsormidium sp. isolated as object, the effect of nitrogen concentration, phosphorus concentration in medium, and light intensity, temperature and environmental pH on the growth, nitrogen consumption, oil content and fatty acid composition of Klebsormidium sp. were investigated. The results showed that nitrogen concentration in the medium did not affect the growth of Klebsormidium sp. , but high nitrogen concentration was detrimental to the synthesis of oil and unsaturated fatty acids. Low phosphorus concentration in the medium inhibited cell growth, while high phosphorus concentration had a toxic effect on cells. Appropriately increasing light intensity was beneficial to cell growth, but had little effect on oil accumulation. More unsaturated fatty acids such as linoleic acid was synthesized under lower light intensity cultivation. Temperature had little effect on the growth and oil accumulation of Klebsormidium sp. , but low temperature was beneficial to the synthesis of linoleic acid. The growth, oil accumulation and linoleic acid content of Klebsormidium sp. were significantly affected by the environmental pH. Cells grew the fastest and had higher oil content in weakly acidic-neutral environments with pH 6.0-7.5. Under the conditions of nitrogen concentration 3-6 mmol/L, phosphorus concentration 0.115-0.230 mmol/L, light intensity 100-300 μmol/(m2·s), temperature 15-30 ℃, environmental pH 7.5, the maximum oil content reached about 42%, of which linoleic acid accounted for 65%-75%. In summary, filamentous microalgae Klebsormidium sp. is a promising new resource for linoleic acid production.

目的探讨H型高血压患病人群睡眠质量及其影响因素，为提高其睡眠质量提供理论依据。方法2017年2月至2018年5月，按性别、年龄及地区分层随机抽取我国14个省的2 609名H型高血压患者，进行睡眠质量及其潜在影响因素的横断面问卷调查。结果最终纳入分析的研究对象共2 587人（男性1 388人，女性1 199人），年龄范围为24至97岁。H型高血压患者睡眠质量差的比例为19.0%，其中，女性睡眠质量差的比例明显高于男性（23.9% vs.14.8%， PPP=0.017］、适量吃肉［OR=0.472；95% CI为（0.306，0.728），P=0.001］及睡眠时长≥8 h/d ［OR=0.008；95% CI为（0.004，0.013），P<0.001］是睡眠质量保护因素；舒张压≥90mmHg［OR=1.441；95% CI为（1.112，1.868），P=0.006］、当地生活水平差［OR=2.542；95% CI为（1.401，4.613），P=0.002］、有慢性肾病史［OR=1.970；95% CI为（1.053，3.687），P=0.034］、日常生活压力大［OR=2.253；95% CI为（1.400，3.626），P=0.001］、动物油作烹调油［OR=1.759；95% CI为（1.056，2.930），P=0.030］及严重鼾症［OR=1.784；95% CI为（1.018，3.126），P=0.043］是睡眠质量危险因素。结论适当体力活动，多食用水果蔬菜，烹调用油选择植物油，适量吃肉，控制血压，减小生活或工作压力、治疗鼾症、充足的睡眠时间可能有助于提高H型高血压患者睡眠质量，进而改善其生活质量。

【摘要】 多时相CTA（multiphase CTA，mCTA）作为一项新型颅内血管影像检查技术，可通过追踪造影剂的空间分布及时间变化动态观察动脉峰值期、静脉峰值期及静脉晚期3个不同时相颅内血管显影情况。其特征性影像——“斑点征”可显示不同时相血管管壁的通透性，评估血栓长度及血栓渗透性，并评估颅内侧支循环状态、病变区血管通透性及静脉溶栓效果。本文就mCTA在侧支循环评估、血管通透性检测及血栓渗透性、血栓长度的识别等方面的研究现状和进展进行综述。 【Abstract】 Multiphase CTA (mCTA), a new imaging technology, can dynamically show three-phase intracranial vascular imaging, including peak arterial phase, peak venous phase and late-venous phase.mCTA is often used to evaluate vascular permeability by characteristic spot sign at different time phases, the state of collateral circulation, and thrombus length and perviousness. The efficacy and prognosis of intravenous thrombolysis can be evaluated by comprehensive imaging features on mCTA. This article reviewed the advances in the application of mCTA in diagnosis and assessment of ischemic stroke, including collateral circulation assessment, vascular permeability, thrombus perviousness and length etc.

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This paper summarized the illness observation and nursing experience in a case of mass diarrhea. Key issues of nursing interventions for diarrhea included illness condition observation, auricular point pressing therapy, moxibustion; psychological care; diet guidance; disinfection and protection, health education and extended care. The integrated traditional Chinese and western medicine nursing is effective to relieve the diarrhea symptoms, reduce the pain degree and improve the rehabilitation of patients. (本文总结1起群体性腹泻的病情观察及护理体会, 包括病情观察、耳穴贴压、艾条灸、心理护理、饮食指导、消毒防护、健康宣教、延续性护理等措施。中西医结合护理能有效缓解患者腹泻症状, 减轻痛苦, 促进患者康复。)

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High quality development of agriculture is an essential requirement of modern agriculture, and the key to agricultural modernization is to achieve agricultural technological innovation, promote high-quality development of agriculture, and technological innovation is the fundamental driving force for agricultural progress. Faced with the era task of technological innovation and high-quality agricultural development, we should deeply integrate technological innovation with multiple fields such as industrial development, green agriculture, and food security. By constructing a full chain technical support system for the agricultural industry, developing diversified and sustainable agriculture, and cultivating agricultural specialty product brands, we can truly leverage the driving role of technological innovation in promoting high-quality agricultural development. [ABSTRACT FROM AUTHOR]

Objective: To investigate effect and mechanism of mangiferin on regulation of M2-type macrophage polarization targeting MMP9 in pancreatic cancer. Methods: In vivo, therapeutic effect of mangiferin on pancreatic cancer was evaluated by drawing tumor growth curves and immunohistochemical staining. M2-type macrophages expression in pancreatic cancer was detected by immunofluorescence and ELISA. Effects of mangiferin on expression of MMP9 and downstream M2 macrophage polarization-related signaling pathways were detected by immunofluorescence, ELISA, Western blot and qRT-PCR. In vitro, MTT assay was utilized to detect effect of mangiferin on M2-type macrophage and therapeutic effect of mangiferin on pancreatic cancer. ELISA was used to detect effect of mangiferin on M2-type polarized macrophages. Effects of mangiferin on expression of MMP9 and its downstream signalling pathway were detected by immunofluorescence and Western blot. Results: Mangiferin had potential to inhibit growth of pancreatic cancer in mice pancreatic model, and could prevent expression of M2-polarized macrophages in pancreatic cancer in addition. At the same time, mangiferin could inhibit expression of MMP9 and downstream M2 macrophage polarization related signaling pathways in pancreatic cancer. Mangiferin inhibited proliferation of pancreatic cancer cells in a M2 type polarized macrophage-pancreas cancer cell co-culture model, inhibited macrophage M2 polarization, at the same time, expression of MMP9 and downstream M2 macrophage polarization related signaling pathway was inhibited. Conclusion: Mangiferin can inhibit macrophage M2 polarization by inhibiting MMP9 and its downstream signaling pathway, and play a role in pancreatic cancer therapy. [ABSTRACT FROM AUTHOR]

Objectives: Length of day variation (ΔLOD) predictions play an important role in tracking and navigation of deep-space detector, precise determination of artificial satellite orbit and climate forecasting. In full consideration of the time-varying characteristics of ΔLOD, we present the application of a hybrid technique for predicting ΔLOD. Methods: The ΔLOD predictions are generated by means of the combination of singular spectrum analysis (SSA) extrapolation for the linear trend, annual and semiannual oscillations in ΔLOD based on an iterative interpolation strategy, and autoregressive integrated moving average (ARIMA) stochastic prediction of SSA remaining residuals, referred to as SSA+ARIMA. In order to evaluate the effectiveness of this approach, the ΔLOD predictions up to 365 d into the future are calculated year-by-year for the 2-year period from Jan. 1,2000 to Dec. 31, 2001 using the data covering the previous 10 years from International Earth Rotation and Reference Systems Service C04 series. The prediction results are analyzed and compared with those obtained by machine learning methods such as back propagation neural network (BPNN), general regression neural network (GRNN) and Gaussian process (GP). Results: It is shown that the accuracy of the predictions are better than that by machine learning methods in terms of the mean absolute error (MAE) of predictions, especially for medium and long-term predictions. Compared with the predictions obtained by the BPNN, GRNN and GP, the MAE of the proposed SSA+ARIMA predictions up to 30 d and 365 d in future is reduced by 39% and 61%, respectively. Conclusions: The proposed method provides a new solution for ΔLOD prediction. [ABSTRACT FROM AUTHOR]

White matter hyperintensity (WMH) is one of the imaging markers of cerebral small vessel disease (CSVD). Recent studies have found certain relationship between WMH and cognitive impairment. In this article, the mechanism of WMH formation, the relationship between WMH and cognitive impairment, the mechanism of cognitive dysfunction caused by WMH were reviewed, aiming to deepen the understanding of the relationship between WMH and cognitive impairment. [ABSTRACT FROM AUTHOR]

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Objective: To investigate the relationship between serum apolipoprotein B (ApoB)/apolipoprotein A1 (ApoA1) ratio, triacylglycerol (TG)/high density lipoprotein cholesterol (HDL-C) ratio, lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) levels and the severity of coronary artery disease in patients with coronary heart disease (CHD) and their predictive value. Methods: 185 patients who were admitted to our hospital for chest pain from January 2019 to December 2021 were selected, and they were divided into CHD group (120 cases) and control group (65 cases) according to the examination results. According to Gensini score, the CHD group was divided into mild stenosis subgroup with 36 cases, moderate stenosis subgroup with 55 cases and severe stenosis subgroup with 29 cases. Serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels were measured after admission. Spearman correlation coefficient was used to analyze the correlation between serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH, ALP level and Gensini score in patients with CHD, and multivariate logistic regression was used to analyze the influencing factors of CHD. The predictive value of serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels on CHD was analyzed by the ROC curve. Results: Compared with the control group, the proportion of smoking, alcohol consumption, hypertension, diabetes mellitus and serum total cholesterol (TC), TG, low density lipoprotein cholesterol (LDL-C), ApoB, ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels in the CHD group were increased, the HDL-C and ApoA1 levels were decreased (P＜0.05). Serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels in mild, moderate and severe stenosis groups were increased successively (P＜0.05). Serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels were positively correlated with Gensini score in patients with CHD (all P＜0.001). Multivariate Logistic regression analysis showed that hypertension, serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels increased were independent risk factors for CHD (P＜0.05). ROC curve analysis showed that the combined prediction of ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels of CHD was larger than alone (P＜0.05). Conclusion: Serum ApoB/ApoA1 ratio, TG/HDL-C ratio, LDH and ALP levels are related to the severity of coronary artery disease in CHD patients, and the value of joint prediction of CHD is high. [ABSTRACT FROM AUTHOR]

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