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In order to screen lactic acid bacteria strains that can be applied as feed additives for fragrant pigs, this experiment isolated and screened a lactic acid bacteria strain with strong acid production ability, good stress resistance, and antibiotic sensitivity from the cecum content of fragrant pigs using 1% calcium carbonate MRS solid culture medium. After Gram staining, physiological and biochemical identification, and 16S rRNA sequencing, the lactic acid bacteria were identified as L. plantarum. Further analysis was conducted on the isolated L. from aspects such as acid production L. performance, acid resistance test, heat resistance test, bile salt resistance test, and antibiotic sensitivity test Plantarum conducts research on biological characteristics. The results indicate that L The optimal temperature for plantarum growth is 37-45 °. L. Plantarum has excellent acid production ability, with a minimum pH of 3.3. L. Plantarum can tolerate low pH and has a survival rate of 13% at a pH of 2.0. The survival rate in MRS broth containing 0.4% pig bile salt was 13%. L. Plantarum is highly sensitive to erythromycin, compound sulfamethoxazole, chloramphenicol, penicillin, ampicillin, and cefazolin. From this, it can be seen that L. Plantarum is a backup strain of probiotics derived from fragrant pigs with excellent stress resistance, an‐ tibiotic sensitivity, and acid production efficiency. [ABSTRACT FROM AUTHOR]

AIM: To evaluate the effect of astaxanthin (AST) on cognitive function of intestinal ischemia/reperfusion (I/R) injury in rats and the role of autophagy. METHODS: Eight-week-old SPF-grade male Sprague-Dawley (SD) rats were randomly divided into sham group, I/R group, AST group and AST+3-methyladenine (3-MA) group, with 12 animals in each group. The superior mesenteric artery (SMA) of the rats in sham group was only exposed without clamping. The SMA in other 3 groups was clamped for 90 min, and then the arterial clamp was released to restore blood supply and perform reperfusion, thus establishing the intestinal I/R model. The rats in AST group were intraperitoneally injected with AST (45 mg·kg−1 ·d−1 )3 d before modeling, and those in AST+3-MA group were intraperitoneally injected with AST (45 mg·kg−1 ·d−1 )+3-MA (1. 5 mg·kg−1 ·d−1 )3 d before modeling. Morris water maze was used to evaluate the cognitive function of rats 48 h after surgery. Hematoxylin-eosin (HE) staining was used to evaluate intestinal tissue damage. Nissl staining of the frontal cortex was used to evaluate neuronal damage. The levels of interleukin-6(IL-6), IL-1β and tumor necrosis factor-α(TNF-α) in the frontal cortex and hippocampus were measured by ELISA kits. The protein levels of beclin-1, microtubule-associated protein 1 light chain 3(LC3) and P62 in the frontal cortex and hippocampus were detected by Western blot. RESULTS: Compared with sham group, the swimming distance of rats in I/R group was increased, with prolonged latency, elevated Chiu's score and decreased number of neurons (P<0. 01), while the levels of IL-6, IL-1β and TNF-α in the frontal cortex and hippocampus were increased (P<0. 01). Beclin-1 expression and LC3-II/LC3-I ratio in the frontal cortex and hippocampus were increased (P<0. 05 or P<0. 01). Compared with I/R group, the swimming distance and latency of rats in AST group were shortened, with decreased Chiu's score, increased neuronal number (P<0. 01), decreased IL-6, IL-1β and TNF-α levels in the frontal cortex and hippocampus (P<0. 01), and increased beclin-1 expression and decreased of P62 expression in the frontal cortex and hippocampus (P<0. 05 or P<0. 01). Compared with AST group, the swimming distance of rats in AST+3-MA group was increased, with prolonged latency, elevated Chiu's score, decreased number of neurons (P<0. 05), increased levels of IL-6, IL-1β and TNF-α in the frontal cortex and hippocampus, and decreased beclin-1 expression and LC3-II/LC3-I ratio and increased P62 expression in the frontal cortex and hippocampus (P<0. 01). CONCLUSION: Astaxanthin alleviates intestinal I/R-induced cognitive impairment in rats by promoting autophagy and inhibiting neuroinflammation. [ABSTRACT FROM AUTHOR]

Objective To understand the relationship between different lifestyles and the formation of fatty liver, and to provide a scientific basis for the prevention of fatty liver. Methods A total of 15 858 physical examination subjects who had no history of fatty liver in the hospital in 2018 were selected as the study objects. A retrospective cohort study was adopted, questionnaire survey and physical examination data were extracted, and Chi-square and logistics regression analysis were used to statistically analyze the influence of different lifestyles on the formation of fatty liver after two years of follow-up. Results The cumulative incidence of fatty liver was 8. 1%, the incidence of male was higher than that of female, the difference was statistically significant(P <0.05), and the incidence of fatty liver increased with the increase of age. The results of univariate analysis showed that men, frequent consumption of high fat, infrequent consumption of milk and soy products, excessive intake of sugar-sweetened beverages, short or long sleep time, smoking and drinking were prone to fatty liver disease(P <0.05). logistics regression analysis shows that high-fat diet and excessive intake of sugary drinks were risk factors for fatty liver(P <0.05), and exercise was a protective factor for fatty liver(P <0.05). Conclusion Keeping a light diet, reducing the intake of sugary drinks and strengthening exercise in daily life can reduce the occurrence of fatty liver. [ABSTRACT FROM AUTHOR]

BACKGROUND: Exercise is an effective method for preventing and treating osteoporosis, but it is unclear whether its effect on postmenopausal osteoporosis is related to changes in bone autophagy levels. OBJECTIVE: To observe the effects of exercise via cellular autophagy on the morphology and mechanical properties of bone tissue in ovariectomized rats, and to explore the mechanism of exercise on bone mass in ovariectomized rats from the perspective of autophagy. METHODS: A rat model of postmenopausal osteoporosis was established, and a 24-week moderate-intensity exercise was used for intervention. After the experiment, serum estradiol levels were measured by ELISA, and bone mineral density and bone microstructure of the cortical and trabecular bone were detected by micro-CT. The biomechanical indicators of the tibia were tested by a three-point bending test. Autophagosomes were observed by transmission electron microscopy. The expression of LC3 and ATG7 proteins was analyzed by western blot. RESULTS AND CONCLUSION: The serum estradiol level in the ovariectomized group was significantly lower than that of the sham-operation group and ovariectomized+exercise group (P < 0.01). The body mass of rats in each group increased, and the order was the ovariectomized group > the ovariectomized+exercise group > the sham-operation group > the sham-operation+exercise group. The bone mineral density and bone mass of rats in all groups significantly increased (P < 0.01), but the increase in the ovariectomized group was significantly lower than that of the other groups, and the increase in the ovariectomized+exercise group was significantly higher than that of the ovariectomized group. Compared with the sham-operation group, the bone mineral density of the tibial cancellous bone in the sham-operation+exercise group was significantly increased (P < 0.01), while the bone mineral density in the ovariectomized and ovariectomized+exercise groups was significantly decreased (P < 0.01). Compared with the ovariectomized+exercise group, the ovariectomized group showed significantly lower bone volume fraction, number of trabeculae, and bone mineral density of cancellous bone (P < 0.05), extremely significantly lower trabecular thickness (P < 0.01), and significantly higher mean trabecular pattern factor, trabecular separation, and structural model index (P < 0.01). Compared with the ovariectomized group, the LC3-II/LC3-I ratio and the relative expression of ATG7 protein significantly increased in the ovariectomized+exercise group (P < 0.05). Compared with the sham-operation and ovariectomized groups, the number of autophagosomes increased in the sham-operation+exercise and ovariectomized+exercise groups, respectively. To conclude, moderate-intensity treadmill exercise can improve the bone microstructure and biomechanical properties of the tibial cancellous bone and increase bone mass in ovariectomized rats by increasing serum estradiol levels and bone autophagy levels. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between serum protein 16（CC16), soluble advanced glycation end product receptor (sRAGE) and the disease outcome in patients with acute respiratory distress syndrome（ARDS) and their predictive value. Methods A total of 127 ARDS patients admitted to the Second People′s Hospital of lingbao city from January 2021 to January 2023 were selected as the research objects, and the patients were divided into the mild group（39 cases), the moderate group（52 cases) and the severe group（36 cases) according to the severity of ARDS. The levels of serum CC16, sRAGE, oxygenation index（PaO2/FiO2), acute physiology and chronic health status Ⅱ（APACHEⅡ) scores and sequential organ failure assessment（SOFA) scores were compared between patients with different severity and patients with different disease outcomes, and the correlation between serum CC16, sRAGE levels and PaO2/FiO2, APACHEⅡ scores, SOFA scores and disease outcomes was analyzed. Logistic regression was used to analyze the risk factors of ARDS patients′ death, and ROC curve was used to analyze the predictive value of combined detection and each individual of serum CC16 and sRAGE levels for ARDS patients′ death. Results Comparison of serum CC16, sRAGE levels, and APACHEⅡ, SOFA scores of patients with different degrees of ARDS at admission: the mild group ＜ the moderate group ＜ the severe group, PaO2/FiO2 comparison: the mild group ＞ the moderate group＞ the severe group, the differences were all statistically significant（P＜0. 05); Serum CC16, sRAGE level, and APACHEⅡ, SOFA scores of the dead patients were higher than those of the surviving patients, and PaO2/FiO2 was lower than that of the surviving patients, and the differences were all statistically significant（P＜0. 05); Serum levels of CC16 and sRAGE were negatively correlated with PaO2/FiO2 on admission, and were positively correlatied with APACHEⅡ, SOFA scores, and disease outcome（P＜0. 05). Serum CC16 and sRAGE levels at admission were risk factors for mortality in ARDS patients. Moreover, the ROC area under the curve of combined detection of two index of the ARDS patients was greater than that of each individual index（P＜0. 05). Conclusion The expression of serum CC16 and sRAGE in ARDS patients is up-regulated, which is closely related to disease outcome, and can be used as an effective indicator for early clinical predictive to outcome. [ABSTRACT FROM AUTHOR]

Aiming at the problems of low model accuracy and poor noise resistance in the traditional damage identification methods for rotating machinery, a multi working condition damage identification method for rotating machinery based on composite multi-scale attention entropy (CMATE) and random forest(RF) was proposed. Firstly, a new nonlinear dynamic tool—composite multi-scale attention entropy, was proposed to measure the complexity of time series. Then, the damage characteristics of vibration signals of rotating machinery were extracted by CMATE to characterize the dynamic characteristics of rotating machinery under different working conditions. Then, the damage features were input into a multi category classifier based on random forest to identify the damage. Finally, three types of rotating machinery datasets, rolling bearings-gearboxes, gearboxes and centrifugal pumps, were used to construct multi-scale condition damage datasets for 9 working conditions and 20 working conditions respectively, and experimental research was conducted on the damage identification method. The results show that the method achieves the recognition accuracy of 95%, 97% and 100% respectively, which is superior to other nonlinear dynamics tools in accuracy and feature extraction efficiency. Moreover, it still achieves good damage recognition results under the noise interference of 0 dB, 1 dB, 2 dB and 3 dB with different signal-to-noise ratios, which proves that the model has remarkable anti-noise property. At the same time, the damage identification method can stably identify the damage of rotating machinery under different loads and speeds, with an average recognition accuracy of 97. 2 % and 93. 5 %, respectively, which has application potential. [ABSTRACT FROM AUTHOR]

The ecological stoichiometry of fine root carbon(C), nitrogen(N), and phosphorus(P)is considered to reflect the strategies whereby plants adapt to the soil environment. In order to gain an understanding of the seasonal variation in fine root C:N:P ratios in different forest types of karst ecosystem, we measured the C, N, and P contents and ratios of living and dead fine roots in shrubland and arbor forest, as well as the influence of abiotic and biotic factors. The results were as follows:(1)C, N, and P contents in the fine roots of arbor forest were higher than those of shrubland, thereby indicating that the fine roots of arbors may by characterized by a stronger absorption and storage of nutrients than those of shrubs. In addition, the contents of C in living fine roots were significantly higher than those in dead fine roots(P<0.05), but N and P contents in living fine roots were lower than those in dead fine roots.(2)It was found that the contents of C in the living and dead fine roots of the two forest types were lower during the rainy season than during the dry season. The N and P contents of living fine roots of shrubs were higher during the rainy season than those in dry season, the opposite pattern was observed for the living fine roots of arbors. During the rainy season, the C:N, C:P, and N:P ratios of living fine roots in shrubland were lower than the values obtained during the dry season. Conversely, the C:N and C:P ratios of the living and dead fine roots of arbors were higher during the rainy season than in the dry season, whereas the values for the N:P ratio were lower during the rainy season than during the dry season. The lower N:P ratios of rainy season tended to indicate that plants in shrubland and arbor forest may be less P restricted during this season.(3)It was found that for both the living and dead fine roots of the two forest types, C contents were higher in trees growing on the upper slopes than in those growing on the middle and lower slopes, whereas the contents of N and P were higher in shrubs growing on the lower slopes and in arbors growing on the middle slopes. Furthermore, the C:N, C:P, and N:P ratios of living and dead fine roots were found to be relatively higher in shrubs growing on the upper slopes. In the case of arbor forest, the C:N ratios of living and dead fine roots in trees growing on the lower slopes were higher than other slopes, however the C:P and N:P ratios in trees growing on the upper slopes were higher than those of trees growing at other slopes, indicating that trees under these two forest types were strongly influenced by P limitation in the upper slopes, but only weakly affected on the middle and lower slopes.(4)Redundancy analysis revealed that forest type, available phosphorus, and season were the main factors influencing the contents and ratios of C, N, and P nutrients in fine roots, and could explain 18.8%, 6.6%, and 6.5% of the observed variation, respectively. These findings indicate that the effects of changes in the N:P ratio associated with differences in forest type, season, and slope should be taken into consideration when vegetation restoration is promoted, which will contribute to accelerating the restoration of karst ecosystems. [ABSTRACT FROM AUTHOR]

Objective To observe the clinical effect of Guo's Bone Setting combined with Huoluo Gukang pills in the treatment of osteoporotic distal radius fracture.Methods A total of 86 patients with osteoporotic distal radius fracture admitted to our hospital from March 2021 to February 2023 were divided into the study group (with even serial number) and the control group (with odd serial number) according to the order of treatment, with 43 cases in each group.The study group was treated with Guo's bone setting manipulative reduction combined with Huoluo Gukang pill, and the control group was treated with Guo's bone setting manipulative reduction combined with Calci D3. Imaging indexes, pain status [visual analogue scale (VAS) score], recovery time, bone mineral density and incidence of adverse reactions were compared between the two groups.Results There were no significant differences in distal radial palmar angle, ulnar deviation degree, radius height, and VAS score between two groups before treatment and on the day of reduction (P＞0.05). The distal palmar angle, ulnar angle, and radius height of the study group were significantly increased at three months after reduction, while those of the control group were significantly decreased, with statistical significance (P＜0.05). After two weeks, one, and three months after manual reduction, the distal palmar angle and ulnar deviation of the radius in the study group were significantly higher than those in the control group, and the radius height was significantly higher than that in the control group, with statistical significance (P＜0.05). Compared with the day of reduction, VAS scores of two groups were significantly decreased at two weeks and one month after reduction, and VAS scores of two weeks and one month after reduction in the study group were significantly lower than the control group, with statistical significance (P＜0.05). The disappearance time of swelling, ecchymosis, and clinical healing time of fracture in the study group were significantly shorter than those in the control group, with statistical significance (P＜0.05). There was no significant difference in bone mineral density of greater trochanteric, femoral neck and Ward triangle between two groups before treatment (P＞0.05). Bone mineral density in all parts of the study group was significantly higher than that in the control group after treatment, and the differences were statistically significant (P＜0.05). The incidence of adverse reactions in the study group was significantly lower than that in the control group, with statistical significance (P＜0.05). Conclusion Guo's Bone setting combined with Huoluo Gukang pills can effectively improve the rehabilitation effect of osteoporotic distal radius fracture, shorten the rehabilitation time and improve bone mineral density without obvious adverse reactions, and it is a safe and effective treatment [ABSTRACT FROM AUTHOR]

This paper takes the rural tourism destinations announced by the Ministry of Agricul‐ ture and Rural Affairs and the Ministry of Culture and Tourism of China as samples, and uses the methods of nearest neighbor index, kernel density and geographic detector to explore the spatial distribution law and influencing factors of rural tourism destinations in China. The results show that: (1) China's rural tourism destinations exhibit the agglomeration characteristics of "more in the east and less in the west, more in the south and less in the north", and the difference between the east and the west is more significant than that between the north and the south. The agglom‐ eration increased gradually. Except for Xinjiang and Qinghai, the nearest neighbor index values of other provinces and cities were 0. 7 to 1. 2, which tended to be evenly distributed. (2) China's rural tourism destinations form a spatial distribution pattern of "two poles-two axes-multiple cores". Of the five types of rural tourism destinations, the leisure and sightseeing type has the highest proportion (33. 06%); and the type of cultural and folk customs has the lowest proportion (8. 28%). The driving factors for the spatial differentiation of the five types of rural tourism des‐ tinations are quite different, which is closely related to the resource endowment and location characteristics of the villages. (3) The spatial differentiation of rural tourism destinations is the result of the combined effect of factors such as the natural environment of the village, the level of social development, the potential of cultural resources, the level of regional facilities, the level of tourism investment, and the scale of the tourism market. The level of social development and the scale of the tourism market are the dominant factors, with explanatory power of 0. 64, 0. 62; factors such as altitude, hydrological conditions and traffic location have a significant negative correlation with the spatial distribution of rural tourism destinations [ABSTRACT FROM AUTHOR]

目的 探讨动机性访谈联合奥瑞姆自护模式对病毒性肝炎患者自我效能感、病耻感、应对方式 的影响。方法 选取濮阳市第五人民医院 2020 年 8 月至 2022 年 7 月收治的 108 例病毒性肝炎患者,按照随机 数字表法分为常规护理组 (54 例) 和联合护理组 (54 例)。两组患者均行对症的药物治疗,常规护理组给予常规 护理,联合护理组在常规护理基础上给予动机性访谈联合奥瑞姆自护模式。干预8周后,比较两组患者治疗依 从率、自我效能感[慢性病自我效能感量表 (CSES)]、病耻感[社会影响量表 (SIS)]、应对方式[医学应对方式问 卷 (MCMQ)]。结果 干预后,联合护理组患者治疗依从率 (98. 15%) 高于常规护理组 (83. 33%),差异有统计 学意义 (P<0. 05); 联合护理组 CSES 量表中疾病共性管理与疾病症状管理 2 个维度评分高于常规护理组,SIS 量表中社会隔离、社会歧视、自我羞耻感、经济歧视 4 个维度评分均低于常规护理组 MCMQ 中回避、屈服 2 个 维度评分低于常规护理组,面对维度评分高于常规护理组,差异均有统计学意义 (P<0. 05)。结论 动机性访谈联 合奥瑞姆自护模式能提高病毒性肝炎患者治疗依从性及自我效能感,促进其积极应对疾病,从而降低病耻感。 [ABSTRACT FROM AUTHOR]

Objective To investigate sodium hydrogen sulfide(NaHS) with function of regulating glutathione(GSH) synthesis to reduce reactive oxygen species(ROS) production in type 2 diabetic cardiomyopathy (DCM). Methods Mouse cardiomyocyte cell line HL-1 was incubated with high concentration of glucose (HG:40 mmol/L) and palmitate (Pal:500 μmol/L) as a cell model of type 2 DCM. HL-1 cells were incubated with NaHS (100 μmol/L), DL-propargylglycin(PPG,1 mmol/L) and N-acetyl-l-cysteine(NAC,5 mmol/L), respectively for 72 hours. The expression of cystathionine-γ-lyase(CSE) and the key enzymes of glutathione production was tested by Western blot. Dihydroethidium(DHE) and dichlorofluoromethane(DCFH) were used to detect the content of ROS in HL-1 cells. Cell viability was detected by CCK8 kit. The content of total GSH was detected. The interaction between muscle specific ring finger protein 1(Murf1) and nuclear factor erythroid-derived 2-related factor 2(Nrf2) and Nrf2 ubiquitylation was determined by co-immunoprecipitation(co-IP). Results Compared with control group, the expression level of CSE,solute carrier family 7 members 11(SLC7A11), glutamate cysteine ligase C(GCLC), glutamate cysteine ligase M(GCLM) and glutathione synthetase(GSS) in HL-1 cells treated incubated with high glucose and palmitate was decreased, however, NaHS was found to restore it. NaHS reduced the content of ROS in HL-1 cells treated with high glucose and palmitate. The interaction between murf1 and Nrf2 was confirmed by co-immunoprecipitation(Co-IP). Compared with NaHS group, the ubiquitylation level of Nrf2 was enhanced in high glucose and palmitate group. Conclusions Sodium hydrosulfide may reduce the ubiquitylation level of Nrf2 and promote the expression of key enzymes of GSH synthesis. [ABSTRACT FROM AUTHOR]

Objective：To explore the potential damage effects and related mechanisms of clozapine (CLP) on the heart development of zebrafish. Method：12.5 μmol/L CLP (L ⁃CLP) and 25.0 μmol/L CLP (H ⁃CLP) were used to culture zebrafish embryos for 72 hours， respectively. The heart morphology，heart rate，and the distance between venous sinus and arterial bulb (SV ⁃BA) were observed under microscope；The expression of cardiac development⁃related genes NKX2.5，Hand2 and Cmlc2 were detected by fluorescence quantitative PCR；In addition，after 24 hours of culture，the endoplasmic reticulum inhibitor 4⁃phenylbutyric acid (4⁃PBA) or dopamine D2 receptor inhibitor bromocriptine (BRC) were added to the H ⁃ CLP group for 48 hours. Then，we counted the cardiac function indicators，and detected the expression of endoplasmic reticulum stress genes (Chop、Bip) and apoptosis ⁃ related genes (Caspase3，Bax/Bcl2) . Results： CLP induced damages of cardiac structure and function in a dose⁃dependent manner，induced the expression of Chop and Bip，and further increased the expression of Caspase3 and Bax/Bcl2. 4⁃PBA and BRC significantly alleviated CLP⁃induced cardiac injury and decreased the expression levels of Chop、Bip、Caspase ⁃3 and Bax/Bcl2. Conclusion：CLP can damage the heart development of zebrafish，and the mechanism may be related to inhibiting dopamine D2 receptor and stimulating endoplasmic reticulum stress. [ABSTRACT FROM AUTHOR]

Objective To evaluate the fairness of vaccination services in Shandong Province, and to provide a basis for further adjusting and optimizing the rational allocation of vaccination institutions and human resources. Methods The comprehensive management data in the immunization planning system of Shandong Province were used to analyze the service accessibility of vaccination institutions and personnel, and the fairness was analyzed by Gini coefficient and Theil index. Results There were 2 422 vaccination institutions in Shandong Province, with a total of 19 497 vaccination personnel, with an average of 42 000 persons per vaccination institution and 5 200 persons per vaccination personnel. The Gini coefficient of service accessibility of vaccination institutions and vaccination personnel in the province was 0.245 and 0.243, respectively, at the "relatively average" level. The Gini coefficient of service accessibility of vaccination personnel in Jinan, Dongying, and Liaocheng was more than 0.4, which was at the level of "big gap". The Theil index of service accessibility of vaccination institutions and vaccination personnel in the province was 0.000779 and 0.045370, respectively. The difference among cities was mainly due to the uneven distribution of resources among counties and districts. Conclusion The allocation of vaccination institutions in Shandong Province is reasonable, but the allocation of vaccination personnel needs to be strengthened and optimized, especially within different counties. [ABSTRACT FROM AUTHOR]

Objective　To investigate the expression level of miR-218-5p and LAYN in colon cancer and the effects of their regulatory relationship on the progress of colon cancer. Methods RT-qPCR was used to detect the expression of miR-218-5p in colon cancer tissues, adjacent tissues, and cell lines. Western blot was used to detect the expression level of LAYN protein in colon cancer tissues, adjacent tissues, and cell lines. The in situ hybridization（ ISH） was used to detect the expression of miR-218-5p in colon cancer and adjacent tissues. Immunohistochemical（IHC） experiment was used to detect the distribution and expression of LAYN protein in colon cancer tissues and adjacent tissues. The dual luciferase experiment was used to detect the binding relationship between miR-218-5p and 3'- rn-translation region（ UTR） of LAYN. CCK-8 was used to detect the cell proliferation. Transwell was used to detect the invasion ability of cells. The flow cytometry was used to detect the apoptosis level of cells. Western blot was used to detect the expression level of cleaved Caspase3 in cells. Results The expression of miR-218-5p decreased in colon cancer tissues and colon cancer cell lines， with the lowest expression in HT-29 cells（P < 0.000 1），and the expression of LAYN increased and the highest expression in HT-29 cells（ P < 0.05） . The double luciferase reporter gene experiment（ P < 0.01） combined with Western blot experiment（P < 0.01） showed that miR-218-5p could bind to the 3'UTR region of LAYN and inhibit its protein expression. In cell experiments， overexpression of miR-218-5p inhibited cell proliferation（ P < 0.05） ， invasion（P < 0.001），and increased apoptosis（P < 0.01），and the expression of cleaved Caspase3 protein（P < 0.01）. Overexpression of LAYN promoted cell proliferation（P < 0.05），invasion（P < 0.001），and decreased apoptosis（P < 0.000 1） and the expression of cleaved Caspase3 protein（ P < 0.01），while overexpression of miR-218-5p and LAYN partially reversed the above changes. Conclusion　The expression level of miR-218-5p is decreased in colon cancer tissues. Cell experiments show that miR-218-5p inhibits cell invasion and proliferation and promotes cell apoptosis through targeted regulation of LAYN. [ABSTRACT FROM AUTHOR]

To investigate the function of sodium hydrosulfide (NaHS) to regulate mitochondrial fusion/fission in diabetic cardiomyopathy and underlying mechanism. Methods Dh/db mice as type 2 diabetes animal model were treated by NaHS. H9C2 cells incubated with glucose (40 mmol/L), palmitic acid (200 µmol/L, Pal) and oleate (200 µmol/L, Ole) were intervened by NaHS (100 µmol/L). H2C9 cells were divided into control, HG+Pal+Ole, HG+Pal+Ole+NaHS and Pal+Ole+DJ-1 siRNA+NaHS groups. The protein level of Mfn2, Fis1, CSE, and DJ-1 was determined by Western blot. Mitotracker staining was used to observe the morphology of mitochondria. The ultra-structural alteration of cardiac tissues was detected by transmission electron microscopy. The cardiac functions were detected by echocardiography. Results Expression of Fis1 was increased (P<0.05) and expression of Mfn2 was decreased (P<0.05) in db/db and H9C2 treated by HG+Pal +Ole compared to control group. NaHS could upregulate the expression DJ-1, enhance the expression of Mfn2. and reduce the expression of Fis1. In db/dh mice, cardiac systolic function was reduced. Disordered arrangement of myofilament, loss of cristae and mitochondrial fission were observed. NaHS could ameliorate these alterations. Conclusions NaHS may alleviate mitochondria injury by promoting mitochondrial fusion. [ABSTRACT FROM AUTHOR]

In order to promote the high-quality development of eight cities in the Henan section of the Yellow River Basin, a high-quality development evaluation system was established for the eight cities in this region based on statistical data from 2011 to 2020. The evaluation system analyzed the development from five dimensions of green, coordinated, open, innovative and harmonious. The comprehensive entropy method was used to calculate the high-quality development index of the eight cities, and the Pearson correlation coefficient was applied to study their correlations. The results show that although the high-quality development index of the eight cities has been increasing, there are still deficiencies in the aspects of coordination and innovative development, and the indexes have not exceeded 0.800. However, the achievements in open and harmonious development are relatively balanced, and the high-quality development indexes of these cities show a positive correlation trend. In promoting high-quality development, it is necessary to focus on improving coordination and innovation, further refine development strategies and policies, and promote coordinated development in various aspects such as economy, environment, and society. [ABSTRACT FROM AUTHOR]

Objective A novel technique for the identification of gas-to-liquid (GTL) lubricants based on mutual information method and Bayesian algorithm is proposed to enhance the tariff identification of lubricating oils. Methods First, feature parameters of GTL lubricants and conventional petroleum lubricants are extracted, and the mutual information algorithm is used to select feature indicators with discriminative ability. Then, the Bayesian algorithm is used to model the selected feature indicators, which finally achieves the classification discrimination of GTL lubricants and conventional lubricants. Results The experimental results demonstrate the effectiveness of the proposed method in accurately discriminating between GTL lubricants and conventional petroleum lubricants. Conclusions The established model exhibits good stability and reliability, providing a comprehensive and accurate identification tool for the lubricating oil industry and tariff assessment. [ABSTRACT FROM AUTHOR]

Objective: To investigate the correlations of age, diseases and blood pression (BP). Methods: A retrospective study was conducted to include 82 782 patients who underwent physical examination in the physical examination department of Hudong Hospital in Shanghai from January 2016 to June 2022 and met relevant criteria. Grouping according to age stages, with each stage representing 10 years, the groups are divided into ≤20 years, 21-30 years, 31-40 years, 41-50 years, 51-60 years, 61-70 years, 71-80 years, and ≥81 years. Pearson bivariate correlation method was used to analyze the correlation between BMI and systolic and diastolic blood pressure. The data were screened again and classified according to the physical examination results. The physical examination population was divided into control group and disease group, and the correlation between hypertension and disease factors was analyzed by Logistic analysis. Results: Systolic blood pressure tended to increase with age, and the level of systolic blood pressure in the group aged 61-70 was consistently higher than in the younger age groups (P < 0.01). Diastolic blood pressure also showed an increasing trend with age, with the highest levels observed between the ages of 51 and 60, followed by a significant decrease. Logistic analysis results indicated that after adjusting for age and BMI factors, metabolic syndrome had a statistically significant impact on isolated systolic blood pressure (OR = 1.068, P = 0.042). Furthermore, metabolic syndrome had a statistically significant effect on both systolic and diastolic blood pressure elevation in hypertension (OR = 1.032, P = 0.038. Conclusion: Blood pressure changes have age specificity, with systolic blood pressure showing a continuous increase with aging. Diastolic blood pressure reaches its highest value between the ages of 51 and 60, and then gradually decreases. Metabolic syndrome is associated with the occurrence of hypertension. [ABSTRACT FROM AUTHOR]

To studied the effect of different ratio of Herba Artemisias Capillaris and Rhubarb on chlorogenic acid and anthraquinones, and to selected the best compatibility ratio, so as to provided effective reference for the development of new drugs containing "YinchenRhubarb". Three different compatibility ratios of Herba Artemisias Capillaris and Rhubarb at 1∶1, 1∶2 and 2∶1 were selected, and then Rhubarb was decocted according to Rhubarb at the same time. Part of the water decoction was selected to prepare Chlorogenic acid and anthraquinones according to the alcohol precipitation method with 70% and 80% alcohol precipitation concentration, respectively. The contents of Chlorogenic acid and anthraquinones in the supernatant and precipitation were determined by HPLC. The results showed that the content of chlorogenic acid increased after the compatibility of Artemisia Chinensis and Rhubarb, and the best compatibility ratio of water decoction and alcohol precipitation was 1∶1, and the content of chlorogenic acid extracted was 55.96 mg/mL. The content of anthraquinones also increased after the compatibility of Artemisia Chinensis and Rhubarb, in which the content of Rhein increased most significantly, and the content of Rhein extracted was 20.83 mg/mL, and the best compatibility ratio of water decoction method and water decoction and alcohol precipitation method was 1∶2. The content of Chlorogenic acid and anthraquinones increased when the compatibility ratio was 1∶1 and the ratio of anthraquinones was 1∶2 after compatibility of Herba Artemisia and Rhubarb. [ABSTRACT FROM AUTHOR]

Digitalization of rural governance is an inevitable trend of future rural governance modernization. Digital empowering rural governance is the policy practice of digital rural construction, the actual needs of solving the problem of rural governance, and a powerful proof of the feasibility of digital technology. At present, the three major areas of Shaanxi Province have made useful explorations in digital empowering rural governance, and formed a distinctive digital governance model in northern Shaanxi, Guanzhong and southern Shaanxi, and achieved many governance results, but also faced some practical problems, such as imperfect digital infrastructure of rural governance, narrow application scenarios of governance and prominent burden of digital technology. These problems restrict the improvement of the effectiveness of rural governance to a certain extent. Therefore, it is necessary to combine the actual situation of the region to solve these development problems and drive village governance of the new era with digital technology. [ABSTRACT FROM AUTHOR]

The scientific characterization of the properties of mesoporous materials is a key link for their development and application. In this paper, the development history, application scope, and characteristics of mesoporous adsorption and commonly used classical analytical models are briefly introduced by tabular comparison. To systematically understand the classical mesoporous analysis methods in physical adsorption, they were classified into four categories: modeled, model-free, completely model-free, and reverse methods. Then, these methods and three frequently-used analytical software for mesoporous adsorption were compared and analyzed. This has reference value for technicians of adsorption instrument management as well as for teaching and research of mesoporous materials. [ABSTRACT FROM AUTHOR]

In order to reasonably evaluate the impact of drought on desert grassland watersheds and accurately judge the transmission characteristics between meteorological drought and hydrological drought, taking the Aibugai River Basin as a typical research area, based on the monthly precipitation and runoff data of 57 years from 1965 to 2021, standardized precipitation index(SPI) and standardized runoff index(SRI) at three different time scales were calculated, and the evolution process, trend characteristics and correlation of meteorological drought and hydrological drought were analyzed. The results show that the application of SPI and SRI can well reflect the changes of meteorological drought and hydrological drought. The sensitivity of SPI value and SRI value to precipitation and runoff decreased with the increase of time scale, and the larger the time scale, the higher the frequency of high-grade meteorological drought events and hydrological drought events. The trend of meteorological drought events and hydrological drought events is increasing, and the degree and likelihood of hydrological drought are more severe than meteorological drought. The larger the time scale, the better thecorrelation between hydrological drought and meteorological drought, and the hydrological drought lags behind meteorological drought, with a lag of 0 ~ 1 month. The research results provide effective theoretical support for drought resistance and disaster reduction in desert grassland basins. [ABSTRACT FROM AUTHOR]

This paper briefly compares the source, application scope, development history and respective characteristics of the commonly used classical models of microporous adsorption. In order to facilitate the systematic understanding and mastery of classical microporous analysis methods in physical adsorption, the methods are divided into three categories: (1) methods developed from standard isotherms, including t-method, αs-method, n-method and MP method; (2) methods based on micropore filling theory, including D-R method, D-A method and D-R-S method; (3) the currently commonly used methods include the original HK method (slit pore), HK-SF method (cylindrical pore), HK-CY method (spherical pore) and the improved HK method. [ABSTRACT FROM AUTHOR]

BACKGROUND: Decreased expression of neurotrophic factors and increased expression of regenerating inhibitors are the main mechanisms of nerve injury. It has been proven that mitochondrial fission inhibitor-1 can decrease clinical score and relieve pathological injury in a mouse model of multiple sclerosis. However, its neuroprotective effects still need to be further explored. OBJECTIVE: To observe the state of neurons and axons in a mouse model of experimental autoimmune encephalomyelitis and to evaluate the neuroprotective effect of mitochondrial fission inhibitor-1. METHODS: A mouse model of experimental autoimmune encephalomyelitis was prepared in C57BL/6 mice by immunizing with myelin oligodendrocyte glycoprotein peptide fragment 35-55. Animal models were randomly divided into two groups (n=15 per group): model group and mitochondrial fission inhibitor-1 group. Mice were euthanized and lumbar spinal cord consecutive sections were harvested at day 28 post immunization. Immunofluorescence staining was used to analyze the state of neuronal cell body and synapse, the phosphorylation level of dynamin-related protein 1, and the expression of neurotrophic factor, regeneration inhibitor and its signaling pathway molecules in spinal cord tissue. RESULTS AND CONCLUSION: Compared with the model group, treatment with mitochondrial fission inhibitor-1 increased the number of neurons marked by neuron-specific nuclear protein in the spinal cord tissue, improved neuronal body morphology, increased the length of synaptophysin-positive synapse, decreased the loss of Neurofilament M, and inhibited the phosphorylation of dynamin-related protein 1. Mitochondrial fission inhibitor-1 treatment could significantly promote the expression of glial cell-derived neurotrophic factor, ciliary neurotrophic factor, and brain-derived neurotrophic factor. Mitochondrial fission inhibitor-1 intervention could markedly reduce the expression of myelin-associated glycoprotein, axon growth inhibitory factor A, axon growth inhibitory factor protein receptor, p75 neurotrophin protein receptor, and Rho-related protein kinase II in the mouse model. To conclude, mitochondrial fission inhibitor-1 can inhibit the phosphorylation level of dynamin-related protein 1, increase the expression of neurotrophic factor protein, and reduce the expression of regeneration inhibitor and related signaling pathway molecular proteins, thus alleviating damage to spinal cord neuron cell bodies, axons, and cysts. [ABSTRACT FROM AUTHOR]

Objective To explore the effects of sleep-wake rhythm intervention on blood cells in mice with aplastic anemia and its possible mechanisms of action by observing the changes in peripheral blood cell count and plasma circadian rhythm regulatory factors (MT, NE) in mice with aplastic anemia whose sleep-wake rhythm has been intervened. Methods Male C57BL/6 mice were randomly divided into the experimental groups ［sleep-wake rhythm intervention groups (sleep intervention group, wakefulness intervention group, sleep-wake intervention group) and sleep-wake non-intervention group］ and normal control group. In the experimental groups, we established the models of aplastic anemia in mice, while the mice in the normal control group did not undergo the model induction. The sleep-wake rhythm was maintained normally in the sleep-wake rhythm non-intervention group and the normal control group. The mice in the wakefulness intervention group and sleep intervention group received 24-hour light exposure and 24-hour sleep intervention, respectively. In the sleep-wake intervention group, sleep and wakefulness patterns were reversed for the first 3 days and the opposite in the following 3 days. Each group underwent a 9-day intervention period. Peripheral blood cell counts were measured in all groups on the day before intervention and on the 6th day after intervention. ELISA was used to measure the levels of MT, NE, OX, and PGE2 in the plasma and bone marrow of mice in the experimental and control groups before intervention. Plasma MT and NE levels were measured in each group on the day before intervention and on the 3rd, 6th, and 9th days after intervention. Results ① Comparison of blood cell counts before and after intervention in each group： Prior to intervention, compared with the normal control group, the experimental group had lower WBC, RBC, PLT counts, and HGB levels （all P<0. 01). On the 6th day of sleep-wake rhythm intervention, compared with the normal control group, the sleep-wake rhythm non-intervention group and sleep intervention group had lower WBC, RBC, and HGB levels （all P< 0. 01). The wakefulness intervention group and sleep-wake intervention group had lower WBC and HGB levels, but higher PLT levels in comparison with those of the sleep-wake rhythm non-intervention group （all P<0. 01). Compared with the sleep-wake rhythm non-intervention group, the wakefulness intervention group and sleep-wake intervention group had higher RBC and HGB levels （all P<0. 01). Compared with the sleep intervention group, the wakefulness intervention group and sleep-wake intervention group had higher PLT levels （all P<0. 05). ② Comparison of plasma MT and NE levels before and after intervention in each group： On the 3rd day of intervention, compared with the normal control group, the sleepwake rhythm non-intervention group and wakefulness intervention group had higher plasma MT levels （all P<0. 01). Com‐ pared with the sleep-wake rhythm non-intervention group, the wakefulness intervention group had higher plasma MT level （P<0. 01), while the sleep-wake intervention group had lower plasma MT level （P<0. 05). Compared with the sleep intervention group, both the wakefulness intervention group and sleep-wake intervention group had higher plasma MT levels （both P<0. 05). On the 6th day of intervention, there were no statistically significant differences in plasma MT levels among the groups, but a pattern emerged with the sleep-wake intervention group > sleep-wake rhythm non-intervention group > normal control group. On the 3rd day of intervention, compared with the normal control group, the sleep-wake intervention group had higher plasma NE level （P<0. 05). Compared with the sleep-wake rhythm non-intervention group, sleep intervention group, and wakefulness intervention group, the sleep-wake intervention group had higher plasma NE levels （all P<0. 05). On the 6th day of intervention, compared with the normal control group, the wakefulness intervention group had higher plasma NE level （P<0. 05), while the sleep-wake intervention group had lower plasma NE level （P< 0. 05). On the 9th day of intervention, compared with the normal control group and sleep intervention group, the sleepwake intervention group had higher NE level （both P<0. 05). Conclusions In mice with aplastic anemia whose sleepwake rhythm is intervened, there is an increase in peripheral blood cell count, as well as elevated levels of plasma MT and NE, indicating intervention in both sleep and wakefulness rhythms. Disrupted sleep-wake rhythm intervention may contribute to the elevation of blood cell count in mice with aplastic anemia by regulating MT and NE levels. [ABSTRACT FROM AUTHOR]

Objective To investigate the success rate, complications and influencing factors of extracorporeal membrane oxygenation (ECMO) puncture and catheterization under ultrasound guidance. Methods Clinical cases of veno-arterial ECMO were collected. The success rate and incidence of complications of ultrasound-guided percutaneous catheterization were analyzed. Meantime, gender, age, cardiac function (ejection fraction (EF) < 30%/EF 30%), target artery diameter (< 5.0 mm/ 5.0 mm), success of one-time puncture, hypertension and other variables were included. Univariate and/or multivariate logistic regression analyses were used to analyze the influencing factors of the overall success rate and complications of catheterization in extracorporeal cardiopulmonary resuscitation (ECPR) patients (ECPR group) and non-ECPR patients (non-ECPR group). Results 65 cases of veno-arterial ECMO were included in the study, with an average age of(49.3 ± 18.2) years. 23 cases were assigned in the ECPR group and 42 cases in the non-ECPR group. The overall success rate of ultrasound-guided ECMO catheterization was 86%, 78% in the ECPR group and 91 % in non-ECPR group. 13 cases developed hematoma and 5 cases of distal limb ischemia after puncture.Logistic regression analyses showed that the target artery diameter and the success of one-time puncture were the influencing factors for the success rate of overall puncture and catheterization, and the success of one-time puncture was also the influencing factor for the incidence rate of hematoma (all P < 0.05). In subgroup analysis, the diameter of target artery was found to be a factor affecting the success rate of puncture and catheterization in the ECPR group (P < 0.05), whereas no statistically significant factor was found in the non-ECPR group (all P > 0.05). Conclusions Percutaneous ultrasound-guided ECMO catheterization has a high success rate and safety. For non-ECPR patients and those with target artery diameter of 5 mm, percutaneous ultrasound-guided ECMO catheterization is the optimal option. Successful one-time puncture should be guaranteed, which can not only improve the success rate of final puncture and catheterization, but also reduce the incidence of hematoma. [ABSTRACT FROM AUTHOR]

Studying the effects of expected economic growth targets and environmental constraint targets set by Chinese local governments at the beginning of the year on high-quality development can provide empirical evidence for optimizing expected target management, improving macro-control, and fulfilling governance efficiency in the new development stage to some extent. Therefore, based on the theory of economic growth accounting and the stochastic frontier model, this paper calculated the level of high-quality development at the urban level, extracted the economic growth targets and environmental constraint targets from the Report on the Work of the Government with text analysis, constructed instrumental variables based on the spatial weight matrix, and then discussed the effects of the expected development targets formulated by local governments on high-quality development, conditional heterogeneity, and mechanisms. The results showed that: ① Excessive economic growth targets were not conducive to high-quality development, while environmental constraints targets had a significant role in promoting it and could slow down the erosion effect of excessive economic growth targets on high-quality development. This conclusion still held after solving a series of robustness tests, such as the endogenous bias. ② Heterogeneity analysis found that, with the implementation of the ecological civilization concept, China' s economy could achieve a win-win situation of quantity increase and quality improvement. To better promote high-quality development, the target positioning of cities with good economic foundations should weaken economic increment and strengthen environmental constraints, and the growth targets and environmental constraints set by local governments should be endogenous to their own regional resource endowments and leave appropriate elastic spaces. ③ The mechanism test showed that improving the economic growth targets reduced the level of innovation and raised the amount of new investment in the region, which was not conducive to high-quality development. Environmental constraints could promote technological innovation and limit the blind expansion of new investment, thus promoting high-quality development. Based on the above research conclusions, this paper summarizes policy implications from the aspects of political performance evaluation, formulation of expected development targets, technological innovation, and green development to promote the synergy between economic growth and green development in China and the synchronization of expected target management and high-quality development. [ABSTRACT FROM AUTHOR]

Aiming at the application of energy-saving and energy storage materials for buildings, a series of octadecane/intumescent graphite forming phase change materials with different octadecane content were prepared by vacuum adsorption method, and the optimal content of octadecane adsorption and its properties were explored through microscopic morphological observation, leakage performance test, differential scanning calorimetry analysis and cold-thermal cycle stability analysis. Through the analysis, it is concluded that when the adsorption amount of octadecane is 90 wt%, the comprehensive performance of the octadecane/expanded graphite forming phase change material under the adsorption content is the best, and its enthalpy of molten enthalpy and solidification enthalpy are 188 J/g and 186.9 J/g, which has a higher enthalpy values compared with different composite phase change materials at home and abroad. After heating the oven at 50 ℃ for 2 h, there is no obvious phenomenon of octadecane exudation, and the quality loss rate is less than 1%, indicating that it has good anti-leakage performance and structural stability. [ABSTRACT FROM AUTHOR]

诊断 ST 段抬高型心肌梗死时, 要求心电图上有连续 2 个及以上导联 ST 段抬高, 这 会导致部分不满足该条件的冠状动脉急性闭塞患者延迟治疗, 影响预后。 近年来, 单一导联 ST 段抬高的下壁心肌梗死心电图 Aslanger 征被广泛熟知, 为患者赢得了早期诊断、早期再灌 注的机会。 本文报告 1 例心电图动态演变为 Aslanger 样、经冠状动脉造影证实为回旋支闭塞 伴其余血管狭窄的胸痛患者, 以进一步提高临床对该心电图表现的认识。 [ABSTRACT FROM AUTHOR]

In order to realize the safe operation and long-term service of South-to-North Water Transfer Project (SNWTP), it is significant to avoid adverse effect led by abnormal state of open channel. Considering the fuzziness of evaluation index and one-sidedness of single method, the evaluation index system of open channel was established with objective analysis method. The improved extension-hierarchical analysis model was proposed to determine the subjective weights based on interval quantization in extension theory, then the subjective and objective weights were combined as fusion weight. The advantages of fuzzy comprehensive evaluation method and grey clustering evaluation method were assembled in order to build the grey-fuzzy evaluation method for open channel. Taking the open channel in the Middle Route of SNWTP as an example, the method and model established in this paper were utilized to evaluate its serviceability and the evaluation results prove their validity and feasibility, which can provide a basis for the operation and maintenance of the open channel projects in the Middle Route of SNWTP. [ABSTRACT FROM AUTHOR]

BACKGROUND: Gutta-percha has low yield strength and shows hard and tough mechanical properties. On this basis, a sports protective gear that can be freely shaped according to the body shape has been developed, and it has been preliminarily confirmed that this performance can reduce the damage to athletes caused by external impact forces. OBJECTIVE: To evaluate the effect of gutta-percha splint on fracture fixation. METHODS: Thirty-five adult male New Zealand rabbits were used to establish closed fracture models of the left tibia by the modified three-point stress method. Totally 30 successful models were randomly divided into two groups (n=15) and fixed with gutta-percha splint and gypsum, respectively. The effects of the two external fixation methods were compared by X-ray imaging and fracture healing was observed at 2 hours, 4 and 6 weeks after fixation. After 6 weeks of fixation, the tibia specimens were observed for gross and histological morphology. RESULTS AND CONCLUSION: (1) X-ray films: Clear fracture line was seen 2 hours after fixation, without obvious periosteal reaction. After 4 weeks, the fracture line became blurred, and a large area of spindle low-density callus shadow was seen at the broken end. After 6 weeks, the fracture line nearly disappeared; the callus connected together to form a cortical bone bridge, and the bone marrow cavity was partially canalized, and partially close to recanalization. After 6 weeks, gray value at the fracture site was higher in the gutta-percha splint group than that in the gypsum group (P < 0.05). (2) General observation: In the gutta-percha splint group, except for three rabbits with malunion, the fracture line of the rest of the rabbits basically disappeared; part of the outer callus was absorbed, and the cortical bone density was close to the normal level. In the gypsum group, except for four rabbits with malunion, the fracture line of the rest of the rabbits disappeared, a small part of the outer callus was absorbed, and the cortical bone density was close to the normal level. (3) Histological morphology: In the gutta-percha splint group, new bone trabeculae were thick; the trabecular space was reduced and fused to form lamellar bone, and the medullary cavity had been partially recanalized. In the gypsum group, new bone trabeculae were reticular, forming a woven bone, and a large number of transected small arteries, veins and capillaries could be seen in the medullary cavity. (4) Complications: Within 6 weeks of fixation, the extremity swelling, purple darkening, displacement and malunion, and the number of local pressure ulcers in the gutta-percha splint group tended to be less than those in the gypsum group, but there was no significant difference in statistical analysis (P > 0.05). (5) The results showed that gutta-percha splint could play a similar fixation effect to plaster on the rabbit model of closed tibial fracture. [ABSTRACT FROM AUTHOR]

Kitchen waste has the characteristics of complex components and differences in texture characteristics in rural areas. A control system based on PLC was designed for the screw press pretreatment with automatic pressure regulation of screw press, equipment cleaning, abnormal feeding handling, working condition monitoring, key data collection and other functions. The system could control the screw press device to treat kitchen waste automatically and realize solid-liquid separation and solid reduction. The screw press control system was tested under the conditions of 0-32 ℃ in different seasons all year round. The results show that the control system can realize automatic running according to the system logic requirements. The system can control the device to steady state within 240 s and keep the control accuracy within 5 percent of the setting value. The drive motor operates stably within 38%-50% of the rated current, and the control system can well match the screw device for kitchen waste pretreatment. [ABSTRACT FROM AUTHOR]

BACKGROUND: Endplate chondrocytes are regulated by many factors in the microenvironment of the intervertebral disc and the influence of microenvironmental changes inside the intervertebral disc caused by abnormal stress on endplate chondrocytes still needs to be explored. OBJECTIVE: To observe the exosome secretion of nucleus pulposus cells under cyclic mechanical tension conditions and the effect of exosomes on endplate chondrocytes under this condition. METHODS: Nucleus pulposus cells were isolated and cultured from the nucleus pulposus of patients undergoing lumbar surgery in vitro. The cells were loaded with cyclic mechanical tension by FX-5000T system. Exosomes secreted from nucleus pulposus cells under stress and non-stress conditions were extracted by magnetic bead method. Purity and concentration of nucleus pulposus cells-derived exosomes were identified by flow cytometry. The exosomes were labeled with PKH67 fluorescent dye and incubated with endplate chondrocytes for 24 hours. Endplate chondrocytes cultured alone were used as control. The uptake of exosomes by endplate chondrocytes was observed under inverted fluorescence microscope. Then the apoptosis and activity of endplate chondrocytes were observed. Type II collagen, depolymerized protein like metalloproteinase 5, matrix metalloproteinase 3, bone morphogenetic protein 2, and β-catenin mRNA expression were detected. RESULTS AND CONCLUSION: Under certain cyclic mechanical tension conditions, nucleus pulposus cells could be promoted to secrete exosomes, and the particle concentration of nucleus pulposus cells-derived exosomes was higher than that under non-stress conditions. Under the inverted fluorescence microscope, both stress and non-stress nucleus pulposus cells-derived exosomes could be ingested by endplate chondrocytes. Compared with the control group, the viability of endplate chondrocytes decreased significantly (P < 0.05) and the apoptosis rate increased in the stress group (P < 0.05). Compared with the control group, the mRNA expression of matrix metalloproteinase 3 and bone morphogenetic protein 2 in endplate chondrocytes decreased significantly in the non-stress group (P < 0.05). Compared with the control group, the mRNA expression of type I collagen decreased significantly in the stress group (P < 0.05), while the mRNA expression of depolymerized protein like metalloproteinase 5, matrix metalloproteinase 3 and β-catenin increased significantly in the stress group (P < 0.05). Compared with the non-stress group, the mRNA expression of type II collagen decreased significantly in the stress group, while the mRNA expression of depolymerized protein like metalloproteinase 5, matrix metalloproteinase 3 and bone morphogenetic protein 2 increased significantly in the stress group (P < 0.05). Therefore, abnormal stress could promote the exosome secretion of nucleus pulposus cells, and the exosomes under this condition could inhibit the viability of endplate chondrocytes and cause the disorder of cell matrix synthesis and catabolism. [ABSTRACT FROM AUTHOR]

The ideal seismic isolation device for electric cabinets should be locked under external excitation that does not affect the safety of the structure. When the device is subjected to earthquakes exceeding the structural fortification intensity, it is quickly unlocked to give full play to its seismic isolation capabilities. The acceleration trigger mechanism can switch the state switching between unlock and lock according to the ground motion acceleration. Therefore, it is an effective method to add an acceleration trigger mechanism to the seismic isolation device. In order to study the impact of acceleration trigger mechanism on the isolation performance of electric cabinets, a electric cabinet was selected as research object. A model of seismic isolation trigger electric cabinet was established through finite element software, and seismic time history analysis was performed to compare the isolation efficiency. Results show that the reasonable trigger limit of the acceleration trigger mechanism should be selected as 0.1g (g is gravitational acceleration). The isolation trigger cabinet's maximal acceleration response and seismic isolation rate are almost same as the isolation cabinet. The effect on the seismic isolation performance is minimal. This shows that the seismic isolation device with acceleration trigger mechanism, can be used as an ideal seismic isolation device for electric cabinets. [ABSTRACT FROM AUTHOR]

Objective: To investigate the value of preoperative modified frailty index（m FI） combined with C-reactive protein/albumin ratio（CAR） and neutrophil/lymphocyte ratio（NLR） in predicting postoperative delirium in elderly patients with colorectal cancer.Methods: 352 elderly patients with colorectal cancer who underwent laparoscopic surgery in The First Affiliated Hospital of Zhengzhou University from January 2020 to December 2021 were selected. According to the occurrence of postoperative delirium, the patients were divided into delirium group（63 cases） and non delirium group（289 cases）. Before operation, m FI was used to evaluate the delirium state,C-reactive protein, albumin, neutrophil count and lymphocyte count were detected, and CAR and NLR were calculated. The risk factors of postoperative delirium in elderly patients with colorectal cancer and the value of m FI, CAR and NLR in predicting postoperative delirium in elderly patients with colorectal cancer were analyzed. Results: The m FI, CAR and NLR in delirium group were higher than those in non delirium group（P<0.05）, and the proportion of age, drinking history, cerebrovascular history, psychiatric history and postoperative transfer to intensive care unit（ICU） were higher than those in non delirium group（P<0.05）. Age, m FI, CAR and NLR increased were risk factors for postoperative delirium in elderly patients with colorectal cancer（P<0.05）. The area under curve of combining m FI, CAR and NLR to predict postoperative delirium in elderly patients with colorectal cancer was 0.835, which was higher than 0.688, 0.741 and 0.733 predicted by m FI, CAR and NLR alone. Conclusion: The increase of m FI, CAR and NLR are risk factors for postoperative delirium in elderly patients with colorectal cancer, combined detection of the three indicators is helpful to evaluate the risk of postoperative delirium in elderly patients with colorectal cancer. [ABSTRACT FROM AUTHOR]

Objective:To establish a comprehensive violence prediction model for community management of patients with schizophrenia in Shanghai,and provide theoretical basis and assessment tools for early warning of violence behaviors.Methods:A total of 7944 patients with schizophrenia who completed follow-up were recruited as a training cohort,and 20% of the patients（1603 cases） were selected by the randomized method as a validation cohort. Baseline data were collected in April 2018,and follow-up data were collected in July and October 2018,respectively.Based on the training cohort group data,the related factors of patient violence were investigated by using logistic regression model.The risk scores were given according to the coefficient（β） of risk factors.The area under curve（AUC） of receiver operating characteristic（ROC） was used to evaluate the predict ability of this score model.Furthermore,the score model was assessed in validation cohort to verify external prediction accuracy.Finally,the risk level of the modeling queue was divided by using the constructed model.Results:Age,economic status,employment status,economic status,self-knowledge,adverse drug reactions,social function,psychiatric symptoms involving violence,medication use,past history of causing an accident,recent stress events,and monitoring were considered as the risk factors of community management in patients with schizophrenia.The AUC of the training cohort was 0.78（95% Cl:0.74-0.81）.Subjects with a score higher than 14 had a significantly increased risk of risky behaviors.The sensitivity,specificity and accuracy of the model were 62%,88% and 87% respectively.According to the constructed model,7944 patients with schizophrenia in the community were classified into 4 risk grades.Patients with highest risk,higher risk,general risk and lower risk accounted for 3.2%,11.0%,69.2% and 16.6% respectively.Conclusion:The predictive model based on the comprehensive violence factors of schizophrenia aggressive behaviors in community management has a good predictive ability,and a good application value due to its simplicity and easy operation. [ABSTRACT FROM AUTHOR]

目的 了解新型冠状病毒肺炎疫情影响下上海市精神卫生专科医院心理应急管理现状及面临的主要问题, 为建立健全精神卫生专科医院心理应急管理体系提供对策和建议。方法 采用定量研究和定性研究相结合的方法, 面向上海市18家精神卫生中心的心理危机干预管理人员及272名心理危机干预队伍成员开展问卷调查, 选取24名心理危机干预相关工作人员进行访谈。结果 （1）管理设置方面, 18家医院中仅上海市精神卫生中心设置了独立的心理危机干预办公室, 15家（83.3%）已制定心理应急总体干预方案或预案, 11家（61.1%）已建立健全心理应急管理相关制度, 12家（66.7%）配备心理危机干预工作经费。（2）队伍建设方面, 心理危机干预队员以兼职为主（84.9%), 年龄主要在35～＜45岁（47.8%), 96.3%的队员具有大学本科及以上学历, 所学专业以精神医学为主（40.4%）, 85.7%的队员具有心理咨询师资格证书, 40.1%的队员具有突发公共卫生事件心理危机干预经验, 81.3%的队员能够有效处理创伤后心理问题。（3）心理应急管理过程中存在的主要问题包括队员压力过大、能力水平参差不齐、队伍人手不足和保障机制不完善4个方面。结论 目前, 上海市已初步形成心理危机干预体系, 但在管理机制和队伍建设方面仍存在不足。建议进一步完善心理危机干预体系管理机制和人才队伍建设。 [ABSTRACT FROM AUTHOR]

As a new material, phase change energy storage materials have irreplaceable advantages and application value in building energy conservation and ecological sustainability. The commonly used solid and liquid phase change materials, due to the problem of easy leakage of solid and liquid phase change materials to a certain extent, restrict the development and application of phase change composites. Aiming at the low temperature solid liquid phase change material encapsulation technology, this paper introduces the low temperature phase change latent heat storage material and its phase transformation in packaging technology research status quo. The porous mineral adsorption phase change material, such as diatomite, expanded vermiculite and expanded perlite, and porous carbon based phase change composites based on expanded graphite were given priority to, as well as the preparation of aerogel phase change composite material technology. The load capacity, thermal performance and thermal conductivity of phase change composites are introduced. On this basis, the future research direction of solid and liquid phase variable composites is proposed, in order to provide some reference for the application and promotion of solid and liquid phase variable composites. [ABSTRACT FROM AUTHOR]

The non-contact measurement method is used to measure and evaluate the three-dimensional morphology of zirconia abrasive belts(P120, P320)and ceramic alumina belt(P120). The results show that the protrusion height of the zirconia abrasive belt and ceramic alumina belt is in the normal distribution and right-skewness distribution respectively. The protrusion area of the zirconia abrasive belt and ceramic alumina belt is both in the right polarized distribution. The protrusion height and area of abrasive grains decrease with the decrease of grain size. The protrusion height and area of abrasive grains in the ceramic alumina belt are smaller than those in the zirconia abrasive belt. The geometric shapes of the abrasive grains of the abrasive belts are similar to the spherical-top cone. The grain size has a significant effect on the spherical-top radius, i.e., the smaller the grain size, the smaller the spherical-top radius, but the effect on the cone apex angle is not significant. There are great differences in the spherical-top radius and cone apex angle of the two abrasive belts with the same particle size. [ABSTRACT FROM AUTHOR]

Some short peptides can spontaneously self-assemble into various nanostructures via the synergistic driving forces of non-covalent interactions. These non-covalent interactions，including electrostatic interaction，hydrogen bonding，aromatic interactions and other non-covalent interactions，are usually highly coupled together. Through rational sequence design and proper modification of short peptide molecules，the driving forces could be regulated purposively，and the nanostructures and morphologies of the self-assemblies could be controlled accordingly，and thus so as to achieve the fabrication of peptide-based supramolecular biomaterials and develop their functions. In this paper，the effects of hydrogen bonding，π - π stacking， electrostatic interaction，hydrophobic interaction，metal ion coordination and chiral center on the selfassembly behavior of peptide self-assembly have been reviewed. The driving force regulation strategies， including sequence design，pH and concentration adjustment and metal ion coordination，and the resulted nanostructures have also been discussed. We also make the outlooks on the development of peptide-based supramolecular biomaterials with specific functions in biomedicines and biocatalysis. [ABSTRACT FROM AUTHOR]

Objective To investigate the predictive value of serum intestinal fatty-acid binding protein (I-FABP) and smooth muscle 22 (SM22) levels on intestinal necrosis in patients with acute mesenteric ischemia (AMI). Methods Totally 167 patients with AMI were divided into the intestinal necrosis group（58 cases) and non-intestinal necrosis group (109 cases). The clinical data of AMI patients were collected, and the serum I-FABP and SM22 were detected by ELISA. Multivariate Logistic regression was used to analyze the influencing factors for intestinal necrosis in AMI, and receiver operating characteristic (ROC) curve was used to analyze the predictive value of serum I-FABP and SM22 levels on intestinal necrosis in AMI. Results Multivariate Logistic regression analysis showed that lactic acid, leukocyte count, I-FABP and SM22 were the influencing factors for intestinal necrosis in AMI (all P<0. 05). The area under ROC curve of I-FABP + SM22 in predicting intestinal necrosis of AMI patients was greater than that of I-FABP and SM22 alone (both P<0. 05). Conclusion AMI patients with elevated serum levels of I-FABP and SM22 are prone to intestinal necrosis, and serum levels of I-FABP and SM22 can be used as predictors of intestinal necrosis in AMI. [ABSTRACT FROM AUTHOR]

从上海市心理热线 30 多年的发展历程、接听运行现状、主要做法、实践成效等方面对热线的总体情况进行系统介绍, 并结合实践中面临的新问题为热线服务能力的提升提供策略建议, 即加强队伍建设、重视专项培训、强化部门联动, 旨在总结心理卫生服务的“上海经验”, 探索形成科学化、规范化的超大城市心理热线管理模式, 推动心理热线平台增效提能。 [ABSTRACT FROM AUTHOR]

The problems of neighborhood security governance are highly coupled with the basic functions of the regional security framework, which indicates that building a new regional security framework is a strategic necessity for neighboring countries to improve their security benefits. As a complete ideological system, the Global Security Initiative provides practical solutions and conceptual guidance for building a new regional security framework, which not only helps establish the overall direction of a new neighborhood security framework, but also inspires the selection of practical paths for building the new framework. As a major power in the region, China needs to continue to play the role of a practitioner of new security concepts, a promoter of security cooperation, a provider of security public goods, and a mediator of security hotspots in the process of building a new regional security framework. [ABSTRACT FROM AUTHOR]

Aiming at the shortcomings of normal SPBO, this paper analyzed the psychological characteristics of students ' learning, and put forward an improved student psychology based optimization algorithm ( HSSPBO) using hybrid strategy . Firstly, it took the inverse value of student ' s test scores as the fitness value of the student, and took the fitness value of the best students in the class as the benchmark, and then divided the students in the class into four categories : the best students, the good students, the ordinary students and the students who tried random improvement. Secondly, it used the dynamic switching probability of sine square and cosine square to balance global exploration and local development, so that the global exploration ability and local exploitation ability of the algorithm were improved effectively. Thirdly, by introducing Cauchy mutation strategy to change the local search step size, it improved effectively the local search ability of the algorithm and enhanced the ability of the algorithm to jump out of the local optimization. Finally, the HSSPBO algorithm used Levy flight strategy to make the individual search step length more random and flexible, which effectively enhanced the individual ' s searching ability, and further improved the optimization speed of the algorithm. Through simulation experiments of 12 benchmark functions and comparison with six optimization algorithms, the results show that HSSPBO ' s global search ability has been significantly improved, and it has faster global convergence speed, better optimization accuracy and stability in function optimization. [ABSTRACT FROM AUTHOR]

The topological superfluid state is protected by the energy gap in the bulk, but it can accommodate the gapless Majorana fermions at the edge of the system. The Majorana fermions satisfy non-Abelian statistics and are protected by topology and have good stability, they can carry quantized information and can be used in the study of topological quantum computing. In recent years, theoretical work has predicted the possible topological superfluid states in various systems. Firstly, we introduce the topological superfluid in various optical lattice models. The ultracold atoms of optical lattice have good controllability and universality. It is an ideal model system to realize topological superfluid. Next, we introduce the topological superfluid under the control of spin orbit coupling. The spin orbit coupling effect is an important condition to induce the topological phase, and the artificial spin orbit coupling has been realized in the experiment. Which makes a breakthrough for the experimental observation of topological superfluid. With the improvement of experimental technology in recent years, the topological FFLO superfluid phase, which was difficult to observe in the experiment and ignored by people, has also become a research hotspot. Therefore, we next introduce the topological FFLO superfluid. In addition, we also introduce the progress in other aspects of topological superfluid, including topological superfluid induced by soliton, three-component topological superfluid, topological superfluid with large Chern number, and the high critical temperature of topological superfluid. In the experiment, how to detect and implement topological superfluid is the purpose and significance of our research. Therefore, we introduce the identification and implementation of topological superfluid at the end of the article. [ABSTRACT FROM AUTHOR]

BACKGROUND: Relevant studies have shown that anterior cruciate ligament rupture may be related to the anatomical morphology of the distal femur and proximal tibia. OBJECTIVE: To investigate the correlation between anterior cruciate ligament rupture and the match between the tibial intercondylar eminence and the femoral intercondylar fossa. METHODS: A total of 74 male soldier patients who were admitted at the 904 Hospital of the Joint Logistics Support Force from January 2016 to March 2021 and suffered unilateral knee injuries or discomfort due to non-contact mechanism were selected. According to X-ray and magnetic resonance imaging findings, they were divided into two groups (n=37 per group): an anterior cruciate ligament rupture group and an intact anterior cruciate ligament group. Width index of the intercondylar fossa was detected using coronal magnetic resonance imaging, and the morphology of the intercondylar fossa was observed using axial magnetic resonance imaging. In X-ray, posteroanterior films were used to detect the width, lateral and medial height of the tibial intercondylar eminence. Ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa was used to describe the match between the tibial intercondylar eminence and the femoral intercondylar fossa. RESULTS AND CONCLUSION: There was no significant difference between the two groups in the width of femoral bicondyle, the lateral and medial heights of the tibial intercondylar eminence (P > 0.05). The width of the intercondylar fossa, the width index of the intercondylar fossa, the width of the tibial intercondylar eminence, and the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa in the anterior cruciate ligament rupture group was lower than that in the intact anterior cruciate ligament group (P < 0.05). The A-shaped intercondylar fossa was more common in the anterior cruciate ligament rupture group, and the U-shaped intercondylar fossa was more common in the intact anterior cruciate ligament group. Univariate Logistic regression analysis showed that the width of the intercondylar fossa, the width index of the intercondylar fossa, the morphology of the intercondylar fossa, the parameters of the tibial intercondylar eminence, and the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa were all the influencing factors for anterior cruciate ligament rupture (P < 0.05). Multivariate Logistic regression analysis showed that the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa was an independent risk factor for anterior cruciate ligament rupture (P < 0.05). Receiver operating characteristic curve showed that the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa had a certain predictive value for anterior cruciate ligament rupture. To conclude, the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa is an independent risk factor for anterior cruciate ligament rupture. It has a certain predictive value for anterior cruciate ligament rupture. The worse the match between the tibial intercondylar eminence and the femoral intercondylar fossa is, the higher the risk of anterior cruciate ligament rupture is. [ABSTRACT FROM AUTHOR]

Composite hydrogel particles are prepared by reverse suspension polymerization. Cellulose acrylic acid nanofiber (ACL-CNF) is used as the polymerization axis. Composite hydrogel microspheres P(AAACC) are prepared by reverse suspension polymerization of acrylamide (AM) and 2-acrylamide-2-methylpropanesulfonic acid (AMPS) with ammonium persulfate (NH4)2S2O8 as initiator. The polymerization process, microstructure, swelling, mechanical strength, temperature and salt resistance of P(AAACC) are studied. The results show that the equilibrium swelling ratio is 13.59 g/g at salt concentration of 10 wt% and 30.15 g/g at 120 ℃, which are 2.63 times and 3.15 times higher than that of ordinary hydrogels, respectively. When the compression ratio of composite hydrogel particles is 85%, the recovery of composite hydrogel particles is 84.8%, while the common hydrogel has been broken. TG-DTG analysis shows that the copolymerization reaction between ACL-CNF and monomer occurs, and the thermal stability is improved. After 7 days of aging under high temperature and high salt, the water retention rate of composite hydrogel particles is 92.1%, which is 11% higher than that of ordinary hydrogel, and the mechanical strength is 2.5 times higher in deionized water and 2.79 times higher in salt water. [ABSTRACT FROM AUTHOR]

Earlier Cambrian banded iron construction (BIF) type iron ore is the most important source of iron in China, accounting for more than 60% of the country's iron reserves, of which the BiF type iron ore in the late Neo-Archean period in North China is the most typical. Detailed petrology, geochemistry and isotopic studies were carried out on the Bif and surrounding rocks of the Xiaolai River in the Qingyuan region of Liaoning Province, northeast of Craton, North China, aiming to provide constraints on the source area and tectonic environment. Xiaolaihe BIF is mainly composed of magnetite, quartz and silicates (such as iron perilla pyroxene, etc.), and the SiO and all-iron (FeO) content of the samples varies widely. The AlO content (mass fraction, the same below) in a small number of Xiaolai River BIF samples was higher (>1%), indicating the presence of an input of land-based clastic material during the sedimentation process. The BIF samples, which were not contaminated with clastic substances, showed typical seawater characteristics, including low alO, high field strength elements and transition metal elements, relative loss of light rare earth elements, and enrichment of heavy rare earth elements and Eu positive anomalies. The simulation results showed that the sample matched the PAAS standardized rare earth element dispensing pattern of the seabed high temperature hydrothermal and seawater 1:100 mixed solution, indicating that the hydrothermal activity on the seabed provided heat and substances to the BIF. The mineralization of the Bif of the Xiaolai River comes from the base-super-bedrock rock at the bottom of the Qingyuan greenstone belt leached with high temperature hydrothermal fluid. The surrounding rocks of the Xiaolai River BIF are mainly metamorphic volcanic rocks of the Stone Shed Subgroup and a small amount of metamorphic clastic sedimentary rocks. The geochemical characteristics of clastic sedimentary rocks are controlled by the composition of complex source regions with medium-low degree of weathering under the island arc system (such as Aochang granite, Yingyun diorite and a small amount of bedrock), which is similar to the source area of the Xiaolai River BIF. Based on the geological, geochemical, isotopic, chronological and other characteristics of the deposit, it is believed that the Xiaolai River BIF system was formed in the Post-Arc Basin of the Algoma-type BIF [ABSTRACT FROM AUTHOR]

Objective To investigate the relationship between recent stress events and aggressive behaviors（AGB） in patients with schizophrenia in Shanghai community. Methods A total of 9 520 schizophrenic patients in Shanghai community（Xuhui District，Hongkou District and Jiading District）were included in this study.The demographic characteristics，medication adherence，insight，psychiatric symptoms involving violence，social functioning，family care and recent stress events were collected by questionnaires in Apr 2018. AGB were followed up in Jul and Oct 2018. Multivariate Logistic regression model was applied to analyze the association between the occurrence of recent stress events（none/mild/severe）and AGB. Subgroup analysis was conducted according to age，gender and other characteristics. Results A total of 7 955 subjects were included in the analysis，and the occurrence number of AGB was 203（2.55%） during the 6-month follow-up. After adjusting the related factors，the risk of AGB in patients with mild stress events was 7.18（95%CI：4.17-12.37） times，and the risk of AGB in patients with severe stress events was 9.33（95%CI：2.33-37.41）times，with a reference of patients without current stress events. In the stratification of senior high school，compared to the patients without stress events，the risk of AGB was 21.65（95%CI：8.95-52.40）times higher in patients with stress events in the last 3 months. While in the stratification of junior high school or primary school and below education，the corresponding ratio was 7.37 （95%CI：3.27-16.61） and 2.32（95%CI：0.19-27.88，respectively，Pfor interaction=0.010）. Similarly，in the stratification of regular medication，compared to the patients without stress events，the risk of AGB was 12.61（95%CI：6.95-22.88）times higher in patients with stress events in the last 3 months. While in the irregular medication group，the corresponding ratio was 2.86 （95%CI：0.82-9.97，Pfor interaction=0.015）. Conclusion Recent stress events had a detrimental effect on AGB in the in community schizophrenics in Shanghai，and this effect intensified in patients with secondary education or regular medication. [ABSTRACT FROM AUTHOR]

In order to quantitatively analyze the degree of fusion of old and new asphalt in thermal regeneration, Fourier transform infrared spectroscopy (FTIR) was used to analyze the fusion degree of recycled asphalt pavement (RAP) to study the degree of fusion of old and new asphalt during thermal regeneration. According to the different infrared characteristic peaks of styreneic-block copolymers (SBS) and base asphalt, using SBS as asphalt tracer, a method for quantitative analysis of the degree of fusion between old and new asphalt was proposed based on the layered solution method. The effect of mixing temperature, mixing time and lignin fiber on the fusion degree of new and old asphalt in the thermal regeneration process was studied by this method. The results show that with the increase of mixing temperature and mixing time, the degree of fusion between old and new asphalt also increases, and the addition of lignin fiber also improves the degree of fusion between old and new asphalt. [ABSTRACT FROM AUTHOR]