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Objective To investigate the effect of inhibiting miR-767-5p on migration, invasion and epithelial-mesenchymal transition（EMT）of breast cancer cells. Methods Breast cancer cells were cultured in vitro, and miR-767-5p inhibitor and inhibitor NC were transfected into breast cancer cells. The cells were divided into three groups： Control group, miR-767-5p inhibitor group and inhibitor-NC group. The effects of miR-767-5p inhibitor on the proliferation, invasion and migration of breast cancer cells were detected by CCK-8 assay, Transwell Migration assay and scratch test. Western blot was used to detect the expression levels of migration related proteins MMP-2 and MMP-9. The dual luciferase reporter gene experiment verifies the targeted binding effect of miR-767- 5p and IGF1. RT-qPCR and Western blot experiments were used to detect the expression levels of IGF1 mRNA and protein. Western blot was used to detect the expression level of EMT related proteins. Results CCK-8 assay showed that compared with the control group and inhibitor-NC group, the miR-767-5p inhibitor group significantly inhibited the proliferation of MCF-7 cells（P < 0.05）. Transwell assay showed that the number of invasive cells of breast cancer cells in miR-767-5p inhibitor group was significantly lower than that in control group and inhibitor NC group （P < 0.05）. Scratch test showed that compared with the control group and inhibitor NC group, the migra⁃ tion rate of breast cancer cells in miR-767-5p inhibitor group decreased significantly （P < 0.05）. Luciferase re⁃ porter gene assay showed that miR-767-5p targeted IGF1 binding. Western blot showed that compared with the con⁃trol group and inhibitor NC group, the expression level of E-cadherin protein in the miR-767-5p inhibitor group was significantly increased （P < 0.05）, while the expression levels of N-cadherin, MMP-2, and MMP-9 protein were significantly reduced （P < 0.05）. Compared with the control group and inhibitor-NC group, the mRNA and protein expression levels of IGF1 in the miR-767-5p inhibitor group were significantly reduced （P < 0.05）. Conclu⁃ sion Suppressing the expression of miR-767-5p may inhibit the proliferation, invasion, migration and EMT of breast cancer cells by down-regulating IGF1. [ABSTRACT FROM AUTHOR]

In order to test the energy saving and emission reduction effect of carbon emission trading policy pilot in China, this paper takes 287 prefecture-level cities as research samples from 2007 to 2018, measures the urban energy-environment efficiency from two dimensions, which are energy-environmental performance index and unified energy-environmental performance index, and uses the quasi-natural experiment method of difference-in-difference to examine the effect of carbon emission trading policy on energy environment efficiency and examines its influence mechanism. The results show that the carbon emission trading policy pilot can effectively promote the improvement of urban energy and environmental efficiency and promote urban energy conservation and emission reduction. Further research shows that the effect of carbon emission trading policy pilot is influenced by urban industrial structure, green innovation intensity and factor market development. In addition, carbon emission trading policy has a more significant role in promoting energy-environment efficiency of large-scale cities and old industrial base cities. Therefore, the further promotion process of carbon emission trading policy in China should focus on industrial structure optimization, green innovation incentive and factor market marketization, and focus on large cities, old industrial cities, so as to achieve the improvement of China’s overall energy environment efficiency. [ABSTRACT FROM AUTHOR]

BACKGROUND: The mechanical environment of cartilage with osteoarthritis at all levels has a certain influence on the development of osteoarthritis. Computer simulation provides a method to quantify the mechanical environment of knee joint cartilage, which makes it possible to study the mechanical behavior of osteoarthritis cartilage by finite element method. OBJECTIVE: To study the stress distribution of cartilage and the change rule of fluid velocity in cartilage of healthy adults and cartilage with osteoarthritis at all levels through numerical simulation so as to explore the mechanical behaviors of cartilage with osteoarthritis at gait cycle. METHODS: The knee joint data of healthy adults were obtained by CT and MRI scanning, and the three-dimensional finite element model of the whole knee joint was established by Abaqus software. The software HyperMesh was used to divide the mesh of the total knee joint, and the binding relationship and interaction between internal components of the knee was set. By changing the material properties of cartilage and adjusting the time length of each phase and the angle between femur and tibial plateau, the cartilage load and boundary conditions of healthy adults and osteoarthritis at all levels could be set in the model. RESULTS AND CONCLUSION: (1) The simulation results showed that the mean Mises stress and the change curves of fluid velocity in the femur cartilage and tibia cartilage increased firstly and then decreased in the gait cycle. (2) Compared with healthy adults, the Mises stress of cartilage in patients with osteoarthritis at all levels continually decreased with duration of disease. The flow velocity of fluid in cartilage firstly increased and then decreased when it developed to osteoarthritis grade 3, and the change range of Mises stress and fluid flow velocity of cartilage with osteoarthritis at all levels decreased. (3) Therefore, with the continuous development of osteoarthritis, the fluid flow velocity increased, the Mises stress decreased and the range of joint surface stress changes decreased in a gait cycle, which lead to the weakening of joint bearing and cushioning capacity. [ABSTRACT FROM AUTHOR]

MicroRNAs ( miRNAs) ait involved in ihe pathological and physiological process of life activities, and some of them exist stably and abundantly in serum, plasma, and other body fluids. Previous studies have indicated that there are significant differences in the expression patterns of circulating miRNAs between patients with viral hepatitis, liver fibrosis, liver cirrhosis, hepatocellular carcinoma, drug -induced hepatitis, and other hepatobiliary diseases at different disease stages. Among these circulating miRNAs. some can act as novel noninvasive biomarkers for the early diagnosis, disease progression, and prognostic evaluation of related diseases. The latest research advances in circulating miRNAs for the diagnosis of hepatobiliary diseases are reviewed. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of miR-149-3p in heart tissues of newborn mice with congenital heart disease (CHD) and its effect on differentiation of mouse embryonic teratoma P19 cells. Methods The newborn mouse model of ventricular septal defect CHD was established. On the 19th day of pregnancy, HE staining was used to observe pathological changes of fetal heart. Then qPCR was used to detect the expression levels of miR-149-3p and heat shock protein family B in fetal mouse heart tissue member 6 (HSPB6) mRNA in heart tissue of fetal mice. Dimethyl sulfoxide (DMSO) was used to induce P19 cells to differentiate into cardiomyocytes, and cells on the 0 th, 5 th and 10 th days of differentiation were collected. qPCR was used to detect markers of myocardial differentiation GATA4, cTnT, the mRNA expression level of atrial natriuretic peptide (ANP) and HSPB6 mRNA and miR-149-3p expression levels during the induction of differentiation. P19 cells were infected with miR-149-3p overexpression lentivirus and empty lentivirus. After induction for 10 days by DMSO, the expression of cTnI protein in cells was detected by immunofluorescence staining. mRNA expression levels of myocardial differentiation markers were detected by qPCR. The dual-luciferase reporter gene experiment was used to verify the targeting relationship between miR-149-3p and HSPB6. Results Compared with the normal group, the newborn mice in the CHD group developed cardiac ventricular septal defect, with high expression of miR- 149-3p and low expression of HSPB6 in cardiac tissue (P＜0.01). During the differentiation of P19 cells into cardiomyocytes, the expression levels of GATA4, cTnT, ANP and HSPB6 mRNA were gradually increased, while the expression level of miR-149-3p was gradually decreased (P＜0.05). After 10 days of differentiation, the overexpression of miR-149-3p significantly reduced the differentiation rate of cardiomyocytes, and down-regulated the mRNA expression levels of GATA4, cTnT and ANP (P＜0.01). The dual luciferase reporter gene experiment confirmed that miR-149-3p could target the expression of HSPB6. Conclusion miR-149-3p might inhibit the differentiation of P19 cells in to cardiomyocytes through targeted down- regulation of HSPB6 [ABSTRACT FROM AUTHOR]

Objective To compare the effect of rubber ring versus dental floss combined with hemoclipping assisted endoscopic submucosal dissection (ESD) in the treatment of early gastrointestinal tumor. Methods A total of 103 patients with early gastrointestinal cancer were divided into the rubber band traction group (rubber band group, n=51) and the dental floss traction assist group (dental floss group, n=52) according to different assisted traction techniques during ESD. The basic conditions of the two groups were compared before operation, as well as the amount of submucosal injection, installation time of the traction device, the amount of bleeding during operation, the time of operation, the delayed bleeding after operation, onetime complete resection rate of tumor, days in hospital after operation and complication rate at 72 hours after operation. The degree of postoperative pain (VAS pain Score), follow-up quality of life (SF-36 Score) and complications 12 months after operation were also recorded. Results The intraoperative submucosal injection volume, intraoperative bleeding volume, number of hemostatic clamp detachment, surgical time, complication rate 72 hour after operation and postoperative hospital stay were lower in the rubber band group than those in the dental floss group (P＜0.05). There was no significant difference in the number of delayed postoperative bleeding between the two groups (P＞0.05). After 12 months of follow-up, patients in the rubber band group had mild postoperative pain and good quality of life. The incidence of complications decreased within 12 months after surgery in the rubber band group (P＜0.05). Conclusion Compared with floss traction aid, rubber ring traction aid ESD can shorten the operation time and reduce postoperative complications, which is worth popularizing. [ABSTRACT FROM AUTHOR]

For sparrow search algorithm convergence mid-term local exploration ability is not enough, in path planning path is not smooth and dynamic obstacle avoidance ability is poor disadvantages. Firstly, to solve the deficiency of local exploration ability of the sparrow search algorithm, the population was initialized using chaotic mapping and the discoverer position updated method was optimized using the previous generation global optimal solution with dynamic adaptive weights. Then, a linear path strategy was used to reduce the number of fold points and nodes. Finally, the optimized sparrow search algorithm was fused with the dynamic windowing method for its disadvantages of unsmooth path and poor dynamic obstacle avoidance ability. The experimental results show that the improved sparrow search algorithm and the dynamic window method fusion algorithm balance the global and local discovery capabilities, accelerate the convergence speed of the pathfinding process, optimize the path and significantly improve the obstacle avoidance capability. [ABSTRACT FROM AUTHOR]

Objective To investigate whether miR-200a suppresses cell proliferation by targeting AP-2γ expression, and reveal molecular mechanism that miR-200a functions as a tumor-suppressor in neuroblastoma cells. Methods Dualluciferase reporter gene assay was employed to examine the effect of miR-200a on AP-2γ promotor luciferase activity. Neuroblastoma cells were transfected with miR-200a mimics, and the expressions of AP-2γ mRNA and protein were detected by RT-PCR and Western blot assay. The effects of AP-2γ down-regulation on cell proliferation were observed after AP-2γ shRNA was transfected into neuroblastoma cells. Neuroblastoma cell proliferation was detected by MTS assay after being cotransfected with miR-200a mimics and AP-2γ plasmid. Results Results showed that miR-200a could inhibit proliferation of neuroblastoma cells at cell viability (66.33±5.13) compared with that of control group (100±0), and also miR-200a can bind to the 3′untranslated region of AP -2γ promotor and inhibit its luciferase activity with an inhibit ratio at (0.624±0.051). AP-2γ mRNA and protein expressions were significantly down-regulated when miR-200a was over-expressed in neuroblastoma cells. Furthermore, results showed that shRNA-mediated down-regulation of AP-2γ that suppressed the cell proliferation of neuroblastoma at (62.5±2.4) by comparing with the control group (100±0). Moreover, restoring AP-2γ expression reversed the effect of miR-200a with a cell viability suppression at (92.4±1.4). Conclusion miR-200a suppresses cell proliferation by targeting AP-2γ expression in neuroblastoma cells. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to clarify the genetic background of strawberry hybrids and their parents. [Method] 10 ISSR primers were selected for genetic background analysis of strawberry hybrids and parents. We used the 10 ISSR primers to analyze 12 strawberry cultivars with complex genetic background. [Result] Between the parents, parents and their hybrids, parent and its tissue cultivating variants, the average genetic distances were 0. 3258,0. 2607 and 0. 1981 respectively, and the average genetic similarity coefficient were 0.6551, 0.7478 and 0.7672, respectively. The clustering analysis and multidimensional scaling analysis confirmed that strawberry cultivars had a relatively narrow genetic background between the tested materials, indicating that the selected 10 lSSR primers can be a powerful tool for strawberry genetic background analysis. [Conclusion] The genetic background analysis of strawberry will lay a foundation for germplasm innovation. [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of different dietary carbohydrate ratios on ruminal fermentation parameters and serum biochemical index in Liaoning cashmere goats. Fifteen Liaoning cashmere goats with similar body condition, body weight (52.5±2.1) kg and permanent rumen fistula were randomly divided into five groups with three replicates in each group. The goats were fed with SC/NSC of 0.85, 1.05, 1.28, 1.56 and 1.96, respectively. The experimental period lasted for 25 d. The results showed that with the increase of SC/NSC ratios, ruminal fluid pH value increased significantly (P<0.05). The pH value of 0.85 group was significantly lower than that of 1.28 group, 1.59 group and 1.96 group (P<0.05), and pH value of 1.05 group was significantly lower than that of 1.96 group (P<0.05). The concentration of NH3-N in 0.85 group and 1.05 group were significantly higher than that in other groups (P<0.05). With the increase of SC/NSC ratio in diets, VFA concentrations decreased, and the content of acetic acid increased in rumen fluid. The experiment indicates that with the increase of dietary SC/NSC ratios, ruminal pH value and the content of acetic acid increased, VFA and NH3-N concentrations decreased, and low SC/NSC ratio was beneficial to improve carbohydrate utilization efficiency. [ABSTRACT FROM AUTHOR]

Objective To identify differentially expressed genes (DEGs)in membranous nephropathy (MN)based on microarray data, and to find new potential gene targets for the diagnosis and treatment of MN. Methods The GSE108109 and GSE115857 datasets were downloaded from the Gene Expression Omnibus (GEO)database. The DEGs between healthy controls and MN patients were screened by the GEO2R web tool. By using the David database, DEGs were enriched by Gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG)pathway. Then, the proteinprotein interaction (PPI)network of DEGs was constructed and visualized by using the String tool and Cytoscape software, and key genes were identified by the Degree analysis. The relationship between expression of key genes and clinical features of MN was analyzed by using nephonseq V5 online platform. Results A total of 240 DEGs were screened out, of which 149 were up-regulated and 91 were down-regulated. DEGs involved many functions and expression pathways, such as cell morphology regulation and cytoskeleton construction. In PPI network, 13 key genes were identified, included tumor protein p53 (TP53), phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), KIT proto-oncogene, receptor tyrosine kinase (KIT), quiescin sulfhydryl oxidase 1 (QSOX1), laminin subunit beta 1 (LAMB1), SHC adaptor protein 1 (SHC1), cyclin dependent kinase 2 (CDK2), apolipoprotein L1 (APOL1), nestin (NES), protein tyrosine phosphatase non-receptor type 6 (PTPN6), RNA polymerase II subunit A (POLR2A), protein kinase cAMP-activated catalytic subunit alpha (PRKACA)and latent transforming growth factor beta binding protein 1 (LTBP1). Among them, the expression of PRKACA (r=0. 858, P=0. 006)and LTBP1 (r=0. 799, P=0. 017)was positively correlated with urine protein, and the expression of POLR2A was negatively correlated with urine protein (r=-0. 601, P=0. 008). PRKACA gene expression was negatively correlated with serum creatinine, and POLR2A expression was positively correlated with serum creatinine (r=0. 583, P=0. 011). Conclusions In this study, we find 240 DEGs and 13 key genes by bioinformatics analysis of renal tissue microarray data. These genes may provide potential gene targets for the diagnosis and treatment of MN. [ABSTRACT FROM AUTHOR]

Objective Through the screening of candidate pathogenic gene among family members of a family with familial hypertrophic cardiomyopathy in Yuxi， Yunnan Province， the study is designed to analyze the relationship between genotype and phenotype and to provide an important theoretical basis for the research of molecular genetic mechanism， early screening and early intervention of familial hypertrophic cardiomyopathy. Methods A detailed medical history was collected and physical examination and routine twelve lead electrocardiogram and cardiac ultrasonography examination were performed among the family members. The peripheral venous blood samples were collected for genetic testing. The genetic map was drawn and the genetic characteristics， genotype and clinical phenotype were analyzed. Results In this family， the dominant inheritance mode of hypertrophic cardiomyopathy is X - linked dominant inheritance. Candidate genes screening showed that a missense mutation was found in the GLA， ZFPM2， SCN5A genes and the translated amino acids were changed. Conclusion X - linked dominant inheritance is the main genetic mode of HCM in this family. GLA c.167G> A (p. Cys56Tyr) heterozygous or hemizygous missense mutation may be the major pathogenic mutation in this family with non-obstructive hypertrophic cardiomyopathy. The clinical significance of ZFPM2 c.1332G> C(p.Lys444Asn) heterozygous missense mutation and SCN5A c.5216G> A (p.Arg1739Gln) heterozygous missense mutation in this family is undetermined. [ABSTRACT FROM AUTHOR]

Objective: To explore the distribution of isolated bacteria and its drug susceptibility feature in culture of tissues from tonsil adenoidectomy in children, and provide instructions for the clinical prevention and treatment. Methods: 483 cases of adenoid tonsil samples were collected for bacterial culture, the main isolated bacteria and drug susceptibility feature were analyzed. Results: The positive rate of pathogenic bacteria was 66.43% and 52.88% for tonsil and adenoid samples respectively. There were four main bacteria,including staphylococcus aureus, haemophilus influenze, streptococcus pneumoniae, group A streptococcus, which accounted for20.71%, 15.11%, 10.97%, 6.42% respectively. S. aureus had a resistant rate of 82% to penicillin, of which 21% isolates were MRSA. While for h. influenzae, the positive rate of beta-lactamase was 30.92%, with a high resistant rate of 50.69% and 30.14% to trimethoprim-sulfamethoxazole and ampicillin respectively. Streptococcus pneumoniae had a resistant rate of 32.08% to penicillin, with a high resistant rate of 81.13%, 79.25% and 69.81% to erythromycin, tetracycline, trimethoprim-sulfamethoxazole respectively. For group A streptococcus, no resistance to penicillin was detected, with a high resistant rate of 83.87%, 77.42% and 58.06% for erythromycin,clindamycin, tetracycline. Conclusions: The main isolated potential pathogenic bacteria included s. aureus, h. influenzae, streptococcus pneumoniae, group A streptococcus. There were obvious differences of the drug resistance among them, which could afford good instructions for its clinical prevention and treatment of pediatric tonsil and adenoid infection. [ABSTRACT FROM AUTHOR]

Objective To explore the optimal subsequent therapeutic regimen for newly diagnosed patients with type 2 diabetes mellitus ( T2DM ) after short-term intensive insulin therapy with insulin pump. Methods Eighty-four newly diagnosed T2DM patients with up-to-standard blood glucose after short-term intensive insulin therapy with insulin pump were selected. They were randomly divided into the observation group and control group，with 42 cases in each group. Patients in the control group were administered oral hypoglycemic agents for subsequent treatment， while patients in the observation group received subcutaneous injection of basal insulin for subsequent treatment. The fasting blood glucose ( FBG )，glycosylated hemoglobin ( HbA1c ) and fasting C peptide ( FCP ) were detected before treatment and after 2-year follow-up. Moreover， the BMI， insulin secretion index ( HOAM-β ) and insulin resistance index ( HOAM-IR ) were calculated. Meanwhile， the carotid intima-media thickness ( IMT ) was detected on the same day，and the carotid plaque detection rate was also calculated. Results HbA1c and BMI in both groups after treatment were lower than those before treatment ( all P < 0. 05 )， but the differences after treatment between these two groups were not statistically significant ( all P > 0. 05 ). HOMA-IR in both groups after treatment was lower than that before treatment，while HOAM-β was higher than that before treatment，and the changes in the observation group were more significant ( all P < 0. 05 ). Carotid IMT in the control group after treatment was higher than that before treatment ( P < 0. 05 )，but the difference in the observation group before and after treatment was not statistically significant ( P > 0. 05 ). In addition， carotid IMT in the observation group after treatment was lower than that in the control group after treatment ( P < 0 . 05 ). Differences in carotid plaque detection rate between two groups before and after treatment were not statistically significant ( all P > 0 .05 ). Conclusion Subcutaneous insulin injection in newly diagnosed T2DM patients after short-term intensive insulin therapy with insulin pump is more effective in improving the pancreatic function than the subsequent treatment with oral hypoglycemic agents. [ABSTRACT FROM AUTHOR]

目的分析甲状腺功能亢进症(简称甲亢)患者并发肺动脉高压的危险因素。方法选取126例甲亢患者,其中单纯甲亢患者86例、甲亢合并肺动脉高压患者40例,采用χ2检验进行单因素分析比较两者性别、年龄、甲亢病程、BMI、吸烟史、饮酒史、左心室射血分数(LVEF)、心率、血清总三碘甲状腺原氨酸(TT3)、血清总甲状腺素(TT4)、甲状腺激素(TSH),采用logistic回归对甲亢并发肺动脉高压危险因素进行多因素分析。结果单因素分析结果显示,单纯甲亢患者与甲亢合并肺动脉高压患者性别、年龄、饮酒、LVEF、TT3比较P均<0.05。多因素logistic回归分析显示,甲亢患者并发肺动脉高压的相关因素有年龄(OR=1.068,95%CI:0.862~0.974,P<0.01)、饮酒(OR=0.721,95%CI:1.189~2.135,P<0.05)、LVEF(OR=0.911,95%CI:1.035~1.672,P<0.05)。结论高龄、饮酒、左心室功能减弱是甲亢并发肺动脉高压的危险因素。 [ABSTRACT FROM AUTHOR]