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The experiment was conducted to investigate the effect of shikimic acid on growth performance, diarrhea index, serum immunity and antioxidant indicators of piglets infected with porcine epidemic diarrhea virus (PEDV). A total of 120 clinically confirmed PEDV infected seven-day-old piglets (Duroc × Landrace × Yorkshire) were randomly divided into six groups, with five replicates in each group and four pigs in each replicate. Treatment group 1, group 2 and group 3 were administered 800, 400 and 200 mg/kg of shikimic acid daily to piglets infected with PEDV, treatment group 4 were treated with daily administration of 400 mg/kg chlorogenic acid to piglets infected with PEDV, treatment group 5 were treated with 20 000 units of gentamicin per day to piglets infected with PEDV, treatment group 6 were treated as positive control groups, and another 20 healthy piglets were selected as the blank control group. The experiment lasted for 5 d, and then observed for 3 d. The results showed that compared with control group with positive PEDV infection, the ADG of piglets with treatment group 1, group 2 and group 3 was significantly increased (P<0.05). The cure rate of PEDV infection in treatment group 1 and group 2 reached 85%, and the cure rate in treatment group 5 was 65%. The IgG content in the serum of piglets in group 1, group 2 and group 3 was significantly increased (P<0.05). The activity of SOD, CAT, NO, MPO, GSH-Px and T-AOC in the serum of piglets in group 1, group 2 and group 3 were significantly increased (P<0.05), and the content of MDA and XOD was significantly reduced (P<0.05). The experiment indicates that shikimic acid can improve the growth performance of piglets, reduce diarrhea index, enhance immunity and serum antioxidant capacity, and have a disease resistance and growth promoting effect on PEDV infected piglets. [ABSTRACT FROM AUTHOR]

Pollution of water resources is seriously endangering human health. Bismuth-based photocatalytic materials also show great potential in controlling organic pollution, with the advantages of green, efficient, clean and low cost. This paper briefly introduces the research progress of various bismuth-based photocatalytic materials in the degradation of organic wastewater, summarizes the modification methods of various bismuth-based photocatalysts and the degradation effects of various organic pollutants, and analyzes the factors affecting the efficiency of bismuth-based photocatalytic materials in the degradation of organic compounds in water. The future development of photocatalysis in the degradation of organic wastewater was also explored, and several proposals for the development of bismuth-based photocatalysis materials were presented. [ABSTRACT FROM AUTHOR]

【Objective】According to the demand of scientific layout of cotton planting for climate change, the study used the species distribution model to quantitatively simulate the suitable distribution of cotton in Manas River basin and its possible response to future climate change, which helps understand the ecological suitability and environmental driving mechanism of cotton planting in arid basin.【Method】The study integrated GIS spatial analysis and MaxEnt model, using 61 cotton planting records and a total of 8 environmental factors screened by R software, and analyzed the suitable distribution range and spatial distribution characteristics of cotton in Manas River basin during the period of reference climate（1970-2000） and 2040 period（SSP245 scenario）. The contribution value of environmental factors, replacement importance value and jackknife method were used to quantitatively analyze the key environmental factors affecting cotton distribution. 【Result】（i） Under the reference climate, the moderate and the most suitable distribution areas of cotton accounted for 9.64% and 12.19% of the total area of the basin, mainly distributed in oasis area of 44°-45° north latitude, including 132, 133, 141 and 144 regiments in Shawan county, 149 and 150 regiments in Manas county, Jiahezi Reservoir and Yuejin Reservoir, and 136 regiment in Shihezi city, southern Kuytun city, northern Wusu city and northeast Karamay city;（ii）Mean diurnal range of temperature, soil pH and solar radiation were key factors affecting the suitable distribution of cotton in the basin, with a cumulative contribution rate of 74.4%. The value ranges of the three factors in the suitable distribution areas were 0.50-13.0 ℃, 5.7-8.7 and 1377-1530 KJ/（m².day）;（iii） Compared with the reference climate, the cotton suitable areas showed a significant trend of decreasing and increasing fragmentation during 2040 period, but the overall decreasing area accounted for 1.5% of the total area of the basin, and decreased to the southern and northwestern of the oasis area of Shawan county, the northeastern Karamay city, Manas county and the south edge of the oasis area of Kuytun city.【Conclusion】The results have great significance for scientific layout of crop planting and stable development in arid basin. [ABSTRACT FROM AUTHOR]

The Pearl River Estuary is the core water area of the Greater Bay area of Guangdong, Hong Kong, and Macao. The river net of the Pearl River Delta is dense and staggered, and its sediment transport process is complex due to the influence of runoff and tides. While sediment transport is a key process in river evolution, it is directly related to the flood control water supply and shipping safety of the Greater Bay area. To investigate the extent of the effect of sediment transport on the Greater Bay area, we analyzed the inversion of water surface sediment under tidal action using in-situ data, high-frequency GF-4 satellite image data (including multi temporal images), water quality, and reflection spectrum data from December 28, 2019 and June 20, 2020. The Mean Absolute Percentage Error (MAPE) value for atmospheric correction was 18.85%. We built several sediment inversion models based on the near-infrared, red, and blue band ratio of the GF-4 satellite image data. The best suited models for the flood and dry seasons had MAPE errors of 30% and 23.54%, respectively. We further obtained the continuous dynamic monitoring results and the daily change of sediment transport in the Pearl River Estuary at an interval of 2 h. The high frequency remote sensing inversion results gave a clear picture of the overall sediment transport dynamics of the estuary. Compared with the in-situ sediment concentration, the inversion results were consistent with the variation of sediment concentration in the tidal cycle. Moreover, we analyzed the dynamic characteristics of sediment transport under the influence of runoff tide. We found that with the rising tide, the overall suspended sediment concentration in Lingding Bay decreased considerably, Lingding Bay is a trumpet shaped estuary located outside the Pearl River Estuary. The north area of Neilingding Island is affected by human activities, thus the riverbed is degraded owing to reduced sediment input and reduced sediment resuspension to the surface. This creates a large water area of low suspended sediment content under the rising tide. Under the ebb tide, water with high suspended sediment content diffuses into the Macau Coastal Waters along the west bank of Lingding Bay. However, the southeast sediment diffusion is restricted by the backwaters from the Humen ebb current. Our method provides a new approach for large-scale operational monitoring of sediment transport in complex estuaries; furthermore, it provides a new strategy of cross-validation between the numerical simulation and remote sensing monitoring of sediment transport. [ABSTRACT FROM AUTHOR]

The experiment was to investigate the effect of dietary supplementation of functional fiber and precision feeding on reproductive performance of sows during pregnancy. A total of 300 parturients sows were randomly divided into three groups with 100 replicates in each group and one pig in each replicate: control group, experiment group Ⅰ and experiment group Ⅱ. During pregnancy, sows in control group were fed with basal diet in the 'high-low-high' mode. Sows in experimental group Ⅰ were fed with modified formula diet + 1% functional fiber in 'high-low-high' mode. Sows in experimental group Ⅱ were fed with modified diet + 1% functional fiber, and the feeding amount was dynamically adjusted according to the body condition of sows. The experiment lasted for 157 d. The results showed that the constipation rate of each experiment group was significantly lower than that of control group (P<0.01). The backfat thickness of sows in experimental group was significantly lower than that of control group at 90 d of gestation and before delivery (P<0.01), and the backfat loss of sows in experimental group was significantly lower than that of control group at weaning (P<0.01). The number of live piglets, the number of healthy piglets, the birth weight of the piglets, the average number of weaned heads in each litter, the weight of the weaning litter and the seven-day weaning rate of each experimental group were significantly higher than those of control group (P<0.01). The days from weaning to mating in experimental group were significantly lower than those in control group (P<0.01). Compared with the control group, the feed input cost of sows in experimental group Ⅱ was the lowest, and the feed cost of the sows in experimental group Ⅱ was reduced by 1.24 yuan, and the benefit per litter was increased by 1 966.24 yuan. The studies indicates that the addition of functional fiber and precise feeding in gestational diets of sows can alleviate constipation, adjust the pregnancy, improve reproductive performance, reduce production costs, and increase economic benefits. [ABSTRACT FROM AUTHOR]

Objective: To purify recombinant lentils allergens Len c1 (rLen c 1) and identify the immune activity. Methods: By means of prokaryotic expression system rLen c 1 was produced , then the target protein with Strep Ⅱ tag was purified with the method of affinity chromatography. BALB/c mice were randomly divided into control group (injected by normal saline) and allergen sensitized group (injected by rLen c 1). The mice were immuned by intraperitoneal injection to set up BALB/c mice lentils allergic model, then the total IgE and allergen specific IgE were detected by indirect ELISA to identify the immune activity of recombinant lentil allergen. Results: Len c 1 protein expression was successfully induced by IPTG，which was expressed in the form of inclusion body; with dialysis renaturation and affinity chromatography,the highly purified renatured protein rLen c 1 was gained, and mice sensitization model was established successfully. Compared with the control group of mice, the TIgE and allergen specific IgE significantly increased in sensitized group of mice. Conclusions: Purified rLen c 1 allergen protein with immune activity was obtained to lay a foundation for the preparation of monoclonal antibodies, clinical diagnosis and immunotherapy. [ABSTRACT FROM AUTHOR]

Objective: To purify recombinant lentils allergens Len c1 (rLen c 1) and identify the immune activity. Methods: By means of prokaryotic expression system rLen c 1 was produced , then the target protein with Strep Ⅱ tag was purified with the method of affinity chromatography. BALB/c mice were randomly divided into control group (injected by normal saline) and allergen sensitized group (injected by rLen c 1). The mice were immuned by intraperitoneal injection to set up BALB/c mice lentils allergic model, then the total IgE and allergen specific IgE were detected by indirect ELISA to identify the immune activity of recombinant lentil allergen. Results: Len c 1 protein expression was successfully induced by IPTG，which was expressed in the form of inclusion body; with dialysis renaturation and affinity chromatography,the highly purified renatured protein rLen c 1 was gained, and mice sensitization model was established successfully. Compared with the control group of mice, the TIgE and allergen specific IgE significantly increased in sensitized group of mice. Conclusions: Purified rLen c 1 allergen protein with immune activity was obtained to lay a foundation for the preparation of monoclonal antibodies, clinical diagnosis and immunotherapy. [ABSTRACT FROM AUTHOR]

The orthogonal design was used to optimize the culture conditions, of PPV N strain in IBRS-2 cell cultivated in roller bottles to provide reference for the mass culture of PPV N attenuated vaccine. Affected factors were as follows; the pH value (7. 0, 7.2, 7.4), the time of inoculation (0, 24, 48h), the dose of toxin (0. 10 %, 1. 00 %, 10.00 %) and the time of harvesting (48, 72, 96 h). Results showed that the best culture conditions of PPV N strain IBRS-2 cells cultivated in roller bottles were as follows; pH =7. 2, synchronization inoculation, 0. 10 % inoculation dose, harvesting the virus solution 96 h after inoculation. Virus solution harvest time is the most important factor affecting the virus titer of PPV N strain. [ABSTRACT FROM AUTHOR]

The monoclinic Ca3MgSi2O8（CMSO） ceramics were prepared by the traditional solid-state reaction method. XRD showed that CMSO sintered at 1350-1425 ℃ were all single-phase structures with space group of P21/a, and SEM found that when the sintering temperature was 1400 ℃, CMSO ceramics had the best surface topography and excellent microwave dielectric properties: εr=13.62, Q×f = 42 292 GHz, τf=-48.36×10-6/℃. The relationship between Q×f values and fill fractions and covalent bonds of CMSO ceramics was discussed. The influence of lattice vibration on the internal dielectric loss of CMSO was studied by Raman spectroscopy, and the octahedral distortion of MgO6 was calculated, which explained the internal mechanism of τf change. In summary, CMSO ceramics have good application prospects in the fields of mobile communication base stations, satellite navigation and radar communications.  [ABSTRACT FROM AUTHOR]

Objective To construct a recombinant retroviral vector containing human ZAK-β cDNA. Methods ZAK-β cDNA was amplified from lung cancer A459 cells by RT-PCR with ZAK-β-specific primers. PCR product was cloned into eukaryotic expression vector pMSCV-IRES-GFP, and then sequenced. Results ZAK-β cDNA was successfully amplified by RT-PCR. Expression vector pMSCV-IRES-GFP-ZAK-β was also successfully obtained and confirmed. Conclusion The recombinant retroviral vector bearing human ZAK-β cDNA was successfully constructed, which forms the basis for the further studies on ZAK-β function. [ABSTRACT FROM AUTHOR]

In this paper, SrY0.15Ce0.85O2.925 ceramic materials were prepared by a solid-phase reaction method, and their phase composition, structure, sintering characteristics and microwave dielectric properties were investigated by XRD, SEM and microwave vector network analyzer. As the sintering temperature was 1 450 ℃, the SrY0.15Ce0.85O2.925 ceramics exhibited the optimized comprehensive performances with bulk density of 5.2 g/cm³, relative density of 92.7%, Q×f =21 503 GHz, εr=23.83 and τf=-46.43 ppm/℃, indicating that the SrY0.15Ce0.85O2.925 ceramics are the alternative material for the preparation of modern mobile communication devices. [ABSTRACT FROM AUTHOR]

BACKGROUND: Digital three-dimensional reconstruction technology is gradually applied to orthopedic diseases with the advantages of visualization, accuracy and non-invasiveness, but there is less evidence-based support for its use in artificial hip hemiarthroplasty for intertrochanteric fractures of the femur in the elderly. OBJECTIVE: To investigate the application value and economic effects of digital three-dimensional reconstruction techniques in artificial hip hemiarthroplasty of intertrochanteric fractures of the femur in the elderly. METHODS: One hundred and thirty elderly patients with intertrochanteric femur fractures admitted to Zunyi First People’s Hospital from January 2019 to December 2022 were selected and randomly divided into a control group (n=65) and an observation group (n=65). Artificial hip hemiarthroplasty was performed in both groups. The control group adopted the film template measurement method for manual preoperative planning while the observation group adopted a digital three-dimensional reconstruction technique. Preoperative planning and intraoperative actual application of prosthesis compliance rate, fibrinogen, D-dimer, bilateral femoral eccentric distance difference, bilateral lower limb length difference, Harris hip function score, visual analog scale score, excellent and good rate of hip function, complications, and hospitalization cost were observed in both groups. RESULTS AND CONCLUSION: (1) The proportion of acetabular side and femoral side prosthesis in grade 0 (fully compliant) was higher in the observation group than that in the control group (P < 0.05). (2) Fibrinogen and D-dimer levels in the observation group were lower than those in the control group 3 days after surgery (P < 0.05). (3) The difference in bilateral femoral eccentric distance and the difference in bilateral lower limb length in the observation group were smaller than those in the control group immediately after surgery (P<0.05). The differences in Harris and visual analog scale scores were not significantly different between the two groups preoperatively, 6 and 12 months postoperatively (P > 0.05). There was no significant difference in excellent and good rate of hip function between the two groups 12 months postoperatively (P>0.05). (4) There was no significant difference in the complication rate between the two groups (P > 0.05). The hospitalization cost of the observation group was higher than that of the control group (P<0.05). (5) It is indicated that digital threedimensional reconstruction technology applied in artificial hip hemiarthroplasty of intertrochanteric femoral fracture in the elderly can not only accurately determine the prosthesis type before surgery, but also accurately reconstruct the bilateral lower limbs offline, but its hospitalization cost is high. [ABSTRACT FROM AUTHOR]

Objective：To construct a mouse asthma model induced by mugwort pollen and to explore endotyping， providing methods for subsequent precision treatment. Methods：BALB/c mice were intraperitoneally injected with mugwort pollen extract （MPE） to sensitize， following MPE intranasal challenge to construct MPE allergic asthma murine model. Mice were randomly divided into PBS sensitization and PBS challenge （P-P）， MPE sensitization and PBS challenge （M-P）， MPE sensitization and MPE challenge model （M-M） groups. 24 h after final challenge， mice were performed to examine airway responsiveness； bronchoalveolar lavage fluid （BALF） was harvested for cell counting and statistical classification of inflammatory cells through flow cytometry analysis. Pulmonary slides were collected for pathological examination， including HE， PAS， Masson and α-SMA immunohistochemical staining. ELISA was used to detect levels of IFN-γ， IL-4， IL-5， IL-13， IL-17A in lung tissue and serum， as well as serum total IgE and MPE-specific IgE， IgG1， IgG2a levels. Results：Pathological examination showed higher airway reactivity， more inflammatory cells infiltration around airway， obvious goblet metaplasia， thickening of airway smooth muscles and dramatical fibrin deposition around airway in model group. Total cell numbers of BALF were increased from <1×105 cells/ml in P-P group to >5×105 cells/ml in model group， in which eosinophils were predominant cellular type， levels of IL-4， IL-13， IL-17A in lung and IL-5， IL-13 levels in serum were significantly increased， as well as significant increasing levels of total IgE and MPE-specific IgE， IgG1， IgG2a. Conclusion：MPE-sensitized and challenged mice induces typical eosinophilic asthma featured with elevated eosinophils， as well as secretion of inflammatory factors of type 2 and type 17， IgE， IgG1 and IgG2a subtypes soars to high levels. [ABSTRACT FROM AUTHOR]

目的　探讨不同年龄段支气管哮喘 (简称哮喘) 患者尘螨过敏情况及肺功能的差异。方法　选取哮喘患 者194例，根据年龄分为儿童组 (6～14岁) 49例、青壮年组 (15～44岁) 106例、中老年组 (45～80岁) 39例。使用全自 动体外过敏原诊断仪检测血清总 IgE (tIgE) 水平及屋尘螨、粉尘螨特异性 IgE (sIgE) 阳性情况，尘螨皮肤点刺试验 (SPT) 观察尘螨过敏情况 (以尘螨SPT阳性率表示) ；肺功能测试仪检查肺功能相关指标，包括用力肺活量 (FVC) 、第 1秒用力呼气量 (FEV1) 、用力呼气中期流量 (MMEF) 、用力呼出气体 50%时的最大呼气流量 (MEF50) 、用力呼出气 体 25% 时的最大呼气流量 (MEF25) 的实际值/预计值及 FEV1/FVC (实际值) 。分析肺功能相关指标与年龄、血清 tIgE 水平的相关性。结果　儿童组、青壮年组、中老年组血清 tIgE 水平分别为 (377. 41 ± 239. 78) 、 (313. 47 ± 276. 95) 、 (235. 27 ± 240. 85) IU/mL，中老年组血清tIgE水平低于儿童组 (P<0. 01) ，儿童组、青壮年组比较差异无统 计学意义 (P>0. 05) 。儿童组、青壮年组、中老年组血清屋尘螨sIgE阳性率分别为97. 76% (42/43) 、77. 50% (62/80) 、 34. 38% (11/32) ，血清粉尘螨sIgE阳性率分别为95. 92% (47/49) 、75. 00% (78/104) 、35. 90% (14/39) ，尘螨SPT阳性 率分别为 95. 92% (47/49) 、87. 74% (93/106) 、56. 41% (22/39) ，儿童组、青壮年组、中老年组血清屋尘螨 sIgE、粉尘 螨 sIgE 及尘螨 SPT阳性率均依次降低，组间两两比较 P均<0. 01。各组 FVC、FEV1比较差异均无统计学意义 (P均 >0. 05) 。儿童组、青壮年组MMEF、MEF50、MEF25及FEV1/FVC均高于中老年组 (P均<0. 01) 。肺功能相关指标与 血清 tIgE 水平均无明显相关性 (P 均>0. 05) 。肺功能相关指标中 FEV1、FVC/FEV1、MMEF、MEF50、MEF25 与年龄 均呈负相关关系 (r分别为-0. 203、-0. 499、-0. 401、-0. 343、-0. 361，P均<0. 05) 。结论　儿童哮喘患者较青壮年及 中老年哮喘患者的血清tIgE水平及屋尘螨sIgE、粉尘螨sIgE、尘螨SPT阳性率升高，且肺功能良好。 [ABSTRACT FROM AUTHOR]

There are many fields choose to fuse LiDAR point cloud and camera images for applying. The accurate extrinsic calibration is a prerequisite for fusing their information. The point cloud feature extraction is a key step in extrinsic calibration. However, the low resolution and low quality of point clouds can affect the accuracy of calibration results. To address these problems, this paper proposed a LiDAR and camera extrinsic calibration method based on edge correlation point cloud. Firstly, it extracted the edge correlation point cloud of the calibration plate by using dual echoes. Then, it obtained the corner points from the edge correlation point cloud by the optimization method, where the corner points were compatible with the actual calibrated plate size. Finally, it matched the corner points in the point cloud with corner points in the image, and solved the extrinsic parameter between the LiDAR and the camera by the multi-point perspective method. The experimental results show that the reprojection error of the method is 1. 602 px, which is lower than that of comparison methods, verifying the effectiveness and accuracy of the method. [ABSTRACT FROM AUTHOR]

The present study aimed to establish expert advice on screening indicators of mental health problems in the setting of health examination. According to the advice, it is necessary to include mental health assessment in health examination. The expert advice provided preliminary suggestions on selection of screening indicators of mental health problems in health examination and the procedure of the mental health assessment. The expert advice provided standards and guidance for mental health assessment in the setting of health examination. [ABSTRACT FROM AUTHOR]

Deep learning has gone through breakthroughs in many research fields including computer vision, natural language processing, etc. due to multiple driving factors such as knowledge, data, algorithms and computing power. In addition, it has gradually spawned a number of new research directions with the migration and application as well as cross-integration among various disciplines. Cheminformatics is a discipline that solves chemical problems with the applied informatics methods, and deep learning can be useful since it is very powerful in nonlinear learning. Deep learning model can be used to screen and predict in the data set, and then verify the feasibility of the results based on theoretical calculation. Finally, the results are represented by experiments, which shortens the experimental period, reduces the labor cost and accelerates the intelligence of cheminformatics. This paper briefly introduces the development history and main network model architecture of deep learning as well as the latest research and application status of deep learning in synthesis planning, compound structure-activity relation and catalyst design in recent years, and also discusses and expects the future development direction. [ABSTRACT FROM AUTHOR]

In order to solve the problem that it is difficult to get out of the local optimum to achieve further optimization in large-scale dense Distributed Constraint Optimization Problems (DCOPs). This paper proposed a local parallel search framework (LPOS) for solving algorithms of DCOPs. In LPOS, the Agent searches all the value assignments of its local neighbors in parallel according to its current value assignment to further expand the search of solution space, so as to avoid the algorithm falling into local optimum prematurely. In order to ensure the convergence of the algorithm, this paper designed an adaptive equilibrium factor K to balance the exploration and exploitation ability of the DCOPs solving algorithms. At the theoretical analysis, it proved that the LPOS can expand the search of solution space and the K can achieve the balance. The experimental results demonstrate that the performance of the proposed LPOS is better than the-state-of-the-art algorithms in both low density and high density. Specifically, LPOS significantly superior to the competitors when solving high density DCOPs. [ABSTRACT FROM AUTHOR]

Objective To investigate the influence of dexmedetomidine on expressions of protein kinase c (PKC)γ, calcium/calmodulin-dependent protein kinase (CaMK)Ⅱα and pCaMKⅡα in spinal cord in rats with incisional pain (IP) and remifentanil-induced hyperalgesia. Methods Forty male Sprague-Dawley rats, aged 2-3 months, weighing 240-260 g, were randomly divided into 5 groups (n=8 each): blank control group (group C), remifentanil+incisional pain group (group R+ I), dexmedetomidine + remifentanil + incisional pain group (group D+R+I), dexmedetomidine + remifentanil + incisional pain + phorbol myristate acetate + DMSO group (group D+R+I+P+DMSO) and dexmedetomidine + remifentanil + incisional pain + DMSO group (group D+R+I+ DMSO). The incisional pain rat model was established by a plantar incision in left hind paw. Remifentanil was infused at a rate of 1.2 μg·kg-1·min-1 for 90 min via the caudal vein. Dexmedetomidine was administered subcutaneously at a dose of 50 μg/kg at 30 min before plantar incision. Phorbol myristate acetate and DMSO were intrathecally injected at a dose of 10 μL. Paw withdrawal latency (PWL) to thermal stimulation and paw withdrawal threshold (PWT) to von Frey hair stimulation were measured 24 h before remifentanil infusion (T0) and at 2, 6, 24 and 48 h (T1-4) after intraveonus remifentanil injection. The rats were sacrificed after the last behavioral test and the L4-6 segment of spinal cord was removed to determine the expressions of PKCγ, CaMKⅡα and pCaMKⅡα in spinal cord by Western blot analysis. Results Compared with group C, the value of PWL was significantly shortened and PWT was significantly decreased except T0, and the expressions of PKCγ, CaMKⅡα and pCaMKⅡα were up-regulated in other groups. Compared with group R+I, the value of PWL was significantly prolonged and PWT was significantly increased, the expressions of PKCγ, CaMKⅡα and pCaMKⅡα were down-regulated in group D+R+I and group D+R+I+DMSO. Compared with group D+R+I, the value of PWL was significantly shortened and PWT was significantly decreased, the expressions of PKCγ, CaMKⅡα and pCaMKⅡα were up-regulated in group D+R+I+P+DMSO. Compared with group D+R+I+P+DMSO, the value of PWL was significantly prolonged and PWT was significantly increased, the expressions of PKCγ, CaMKⅡα and pCaMKⅡα were down-regulated in group D+R+I+DMSO. Conclusion Dexmedetomidine can reduce the expressions of PKCγ, CaMKⅡα and pCaMKⅡα in spinal cord in rats with IP and hyperalgesia induced by remifentanil. [ABSTRACT FROM AUTHOR]

Objective: To obtain the biologically active house dust mite allergen Der f 3. Methods: The expression vector p ET44a-Der f 3 contained Strep tag Ⅱfor producing target protein was constructed efficiently. Recombinant protein was renatured by gradient dialysis, analysed by Western Blot and purified by affinity chromatography. The enzymatic activity of Der f 3 was identified by using specific fluorogenic substrates. Plasma from house dust mite allergic patients was used to evaluate immunological activity of Der f 3. Results: The target protein was successfully expressed in prokaryotic expression system. The target band appeared as expected on Western Blot profile. Specific substrates broke down successfully by the action of the actived and purified house dust mite allergen Der f 3. The results of the enzyme-linked immunosorbent assay showed 4 % positive rate of the specific antibody IgE among the plasma of patients suffering from allergy against house dust mite. Conclusions: The house dust mite allergen Der f 3 was expressed successfully with enzymatic activity and immunological activity. So it could be used as diagnostic reagent and vaccine of high quality. [ABSTRACT FROM AUTHOR]

OBJECTIVE: The up-to-now researches on the effect of transcranial direct current stimulation (tDCS) on exercise performance are in the initial phase, and the existing conclusions are inconsistent. This meta-analysis attempted to quantitatively evaluate the effect of tDCS on muscle strength and endurance performance of healthy adults, providing new evidence for new neuroelectrophysiological techniques as well as improving the muscle function. METHODS: To collect randomized controlled trial (RCT) studies regarding the effects of tDCS on muscle strength and endurance performance in healthy adults, the following databases were searched from inception to October 2019: Web of Science, PubMed, Sport Discus, and CNKI. According to the Cochrane Systematic Review Manual, Review Manager 5.3 software was used to evaluate the methodological quality of the literature included and to analyze the combined effect amount. RESULTS: A total of 15 RCTs involving 201 healthy subjects were included. The meta-analysis indicated that compared with the experimental group, tDCS intervention had insufficient evidence in improving muscle strength: I²=47%, 95% confidence interval [-0.79, 0.23], P=0.27 > 0.05, standard mean difference=-0.28. Compared with the control group, the function of tDCS stimulation to improve the performance of endurance was more evident, and the combined effect amount tended to a medium effect: I²=57%, 95% confidence interval [0.10, 0.76], P=0.01 < 0.05, and standard mean difference=0.43. CONCLUSION: tDCS intervention has no statistical significance in improving strength, but it is effective in improving endurance performance in general. More rigorous and extensive experimental researches are warranted to explore the tDCS neurophysiological mechanism. [ABSTRACT FROM AUTHOR]

Objective To investigate the influence of the silence of UBE2C gene on the gastric cancer AGS cells proliferation and migration of human. Methods Using RNA interference technology，the expression vector of UBE2C silenced lentivirus was transfered into 293T cell line with packaging plasmids to produce the lentiviral particles. Lentivirus was used to infect human gastric cancer AGS cells，and the cells with stably expressing UBE2C were obtained by puromycin screening. The experiment was divided into the interference group and the interference control group. The expression levels of UBE2C mRNA in human gastric cancer AGS cells were detected by quantitative real-time polymerase chain reaction （qRT-PCR）. The capacity of AGS cell proliferation and migration was determined with real-time label free cell analyzer （RTCA）. Meanwhile，Cell counting kit-8 （CCK-8） was used to further confirm the effect of UBE2C expression level on the proliferation of human gastric cancer AGS cells. Results Compared with the interference control group，UBE2C shRNA transfected human gastric cancer AGS cells significantly decreased the expression of UBE2C mRNA （P < 0.05）；the cellular proliferation and migration ability were obviously decreased. Conclusion Targeted silencing of UBE2C gene expression can inhibit the malignant biological behavior of human gastric cancer AGS cells. [ABSTRACT FROM AUTHOR]

[Objective] The reverse transcription polymerase chain reaction was developed to detect Poreine teschovirus in swine clinical samples in Guangxi. [Method] Based on sequence analysis of different serotypes in Gen Bank, the RT-PCR primers were designed with conservative regions at the 5’ end, and the RT-PCR assay was established after annealing temperature optimization and specificity and sensitivity-test,and to diagnosis Porcine teschovirus infection in Guangxi. [Result] This detection method had high specificity and it can amplify Poreine teschovirus, no detection appeared with Porcine epidemic diarrhea virus, Poreine pseudorabies virus, Porcine reproductive and respiratory syndrome virus, Classical swine fever virus, porcine transmissible gastroenteritis virus, Group A porcine rotavirus, Group C porcine rotavirus, porcine deltacoronavirus. The detection was high sensitive and its limit was as lower as 1.16 pg/μ1 plasmid template and 12.15 pg/μ1 cDNA template. The established RT-PCR method was used to detect 235 cases of diarrhea feces collected from pig farms in Guangxi from March 2018 to April 2019, and 24 cases were positive for PTV, with a positive rate of 10.21%. [Conclusion] The established RT-PCR was simple, specific and sensitive assay, which can be used in laboratory PTV detection and diagnosis. PTV infection has been found in some pig farms in Guangxi, so we should be vigilant, strengthen monitoring, prevention and control. [ABSTRACT FROM AUTHOR]

Objective:In order to solve the dimension disaster and over-fitting problems in the process of tumor subtype recognition, a particle swarm optimization (PSO) BP neural network ensemble algorithm was proposed.Methods:The Euclidean distance and mutual information was used to preliminarily filter redundant genes, and then Relief algorithm was adopted to further process the candidate feature genes set. The BP neural network was used as the base classifier, which combines feature genes extraction with classifier training.Results:When the number of hidden layer neurons is 5 and the number of candidate feature genes is 110, the QPSO/BP algorithm can optimize and search globally.Conclusion:The algorithm not only improves the accuracy of tumor classification and recognition, but also reduces the complexity of learning. [ABSTRACT FROM AUTHOR]

Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli， then purificate so as to obtain GST-SRY fusion protein. Methods SRY gene was amplified by PCR and cloned into prokaryotic expression vector pGEX-6P-1. pGEX-6P-1-SRY plasmid was verified by restriction enzyme digestion and DNA sequencing. After that，the recombinant plasmid was transformed into E.coli Rosetta DE3. Expression of GST-SRY fusion protein was induced by IPTG，which was purified by Glutathione Sepharose later on. Results Restriction enzyme digestion and sequencing demonstrated that pGEX-6P-1-SRY was successfully obtained. The result of SDS-PAGE assay indicated that SRY was successfully expressed in E.coli Rosetta DE3 and expressed a 49KD SRY protein．Conclusion The successful construction of SRY gene recombinant prokaryotic expression vector and expression of SRY protein will be helpful for further studies of SRY gene function. [ABSTRACT FROM AUTHOR]

Objective To construct the lentiviral expression vector of SOX9 gene and establish a LNcap cell strain with stable expression of SOX9 . Methods SOX9 gene was amplified by PCR and cloned into lentiviral expression vector pLVX-IRES-Puro. pLVX-IRES-FLAG-SOX9 recombinant plasmid was verified by restriction enzyme digestion and DNA sequencing. Then we gained recombinant virus particles in packaging cell HEK 293 and infected LNcap cell. The monoclonal LNcap cell strain stably expressed were obtained through puromycin screening. The mRNA level and protein level in the infected LNcap cell were detected by qRT-PCR and Western blot respectively. Results Restriction enzyme digestion and sequencing demonstrated that SOX9 cDNA was successfully cloned into pLVX-IRES-FLAG lentiviral vector. After the transfection to LNCaP cells， the monoclonal cell strain of stably expressed SOX9 were obtained by puromycin screening， which showed expression of SOX9 mRNA detected by qRT-PCR. Western blot analysis revealed that SOX9 protein expressed markedly. Conclusion The recombinant lentiviral vector bearing human SOX9 cDNA has been successfully constructed，and the exogenous expression of SOX9 in LNcap cells was achieved. [ABSTRACT FROM AUTHOR]

Objective To construct a recombinant vector containing human WDR5 and establish LNCaP cell strain with stable expression of WDR5. Methods WDR5 gene was amplified by PCR and was cloned into eukaryotic expression vector pCI- neo. The resulted pCI- neo- WDR5 plasmid was verified by restriction enzyme digestion and DNA sequencing. LNCaP cells were transfected with pCI- neo- WDR5 by liposome. The expression of WDR5 in the cells was examined by Real- time PCR. Re s ults Restriction enzyme digestion and sequencing analysis showed that WDR5 cDNA was correctly cloned into pCI- neo vector. After transfection of LNCaP cells with the recombinant plasmids, the monoclonal cell strain of stably expressed were obtained by G418 screening，which showed expressions of WDR5 mRNAs detected by Real Time- PCR. Conclusion The recombinant of PCI- neo- WDR5 stably express in the transfected LNCaP cells，which laid a foundation for further study on WDR5. [ABSTRACT FROM AUTHOR]

In order to establish a rapid multiplex RT-PCR assay for detection and differentiation of variant，classical and attenuated vaccine strains of porcine epidemic diarrhea virus (PEDV). Two pairs of specific primers were designed based on S gene sequences and 0RF3 gene sequences of variant，classical and attenuated vaccine strains PEDV published in GenBank，to amplify the partial S gene fragment and ORF3 gene fragment of different PEDV strains，the type of PEDV strain could be differentiated according to the number of the fragment and the size of the fragment. Results showed that the multiplex RT-PCR could detect and differentiate variant， classical and attenuated vaccine strains PEDV. Variant PEDV strain generated two fragments，they were 826 bp of the S gene and 234 bp of the ORF3 gene，respectively. Classical PEDV strain only amplified 234 bp of the ORF3 gene，while attenuated vaccine strain produced 185 bp of the ORF3 gene. The multiplex RT-PCR did not cross-react with TGEV，PRoVA，PKV，PRRSV，PRV，CSFV，PCV-2，JEV，TTV and PPV used in the study. Testing of the sensitivity of RT-PCR indicated as low as 2. 3 x 10 -4 ng/μl nuclear acids could be detected accurately and rapidly. Ninety-one stool specimens collected from different farms in Guangxi Province were detected by the established multiplex RT-PCR，64 samples were positive for PEDV， of which 89. 06 % (57/64 ) was variant PED V strains，4. 69 % (3/64) was classical PEDV strain and 6. 25 % (4/64) was attenuated vaccine PEDV strain. Therefore the multiplex RT-PCR could be used as an effective tool for differentiating diagnosis of PEDV in epidemiological investigations. [ABSTRACT FROM AUTHOR]