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ZAK-γ 蛋白激酶基因真核表达载体的构建.
- Source :
-
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao . 2015, Vol. 36 Issue 7, p1-4. 16p. - Publication Year :
- 2015
-
Abstract
- Objective To construct a recombinant retroviral vector containing human ZAK-β cDNA. Methods ZAK-β cDNA was amplified from lung cancer A459 cells by RT-PCR with ZAK-β-specific primers. PCR product was cloned into eukaryotic expression vector pMSCV-IRES-GFP, and then sequenced. Results ZAK-β cDNA was successfully amplified by RT-PCR. Expression vector pMSCV-IRES-GFP-ZAK-β was also successfully obtained and confirmed. Conclusion The recombinant retroviral vector bearing human ZAK-β cDNA was successfully constructed, which forms the basis for the further studies on ZAK-β function. [ABSTRACT FROM AUTHOR]
Details
- Language :
- Chinese
- ISSN :
- 2095610X
- Volume :
- 36
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Kunming Medical University / Kunming Yike Daxue Xuebao
- Publication Type :
- Academic Journal
- Accession number :
- 110621444