UBE2C基因沉默表达对人胃癌AGS细胞增殖和迁移的影响.

Objective To investigate the influence of the silence of UBE2C gene on the gastric cancer AGS cells proliferation and migration of human. Methods Using RNA interference technology，the expression vector of UBE2C silenced lentivirus was transfered into 293T cell line with packaging plasmids to produce the lentiviral particles. Lentivirus was used to infect human gastric cancer AGS cells，and the cells with stably expressing UBE2C were obtained by puromycin screening. The experiment was divided into the interference group and the interference control group. The expression levels of UBE2C mRNA in human gastric cancer AGS cells were detected by quantitative real-time polymerase chain reaction （qRT-PCR）. The capacity of AGS cell proliferation and migration was determined with real-time label free cell analyzer （RTCA）. Meanwhile，Cell counting kit-8 （CCK-8） was used to further confirm the effect of UBE2C expression level on the proliferation of human gastric cancer AGS cells. Results Compared with the interference control group，UBE2C shRNA transfected human gastric cancer AGS cells significantly decreased the expression of UBE2C mRNA （P < 0.05）；the cellular proliferation and migration ability were obviously decreased. Conclusion Targeted silencing of UBE2C gene expression can inhibit the malignant biological behavior of human gastric cancer AGS cells. [ABSTRACT FROM AUTHOR]