201. Two-Step Production of Neofructo-Oligosaccharides Using Immobilized Heterologous Aspergillus terreus 1F-Fructosyltransferase Expressed in Kluyveromyces lactis and Native Xanthophyllomyces dendrorhous G6-Fructosyltransferase
- Author
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Doreen Gerlach, Markus Baas, Peter Czermak, Jan Philipp Burghardt, and Publica
- Subjects
0301 basic medicine ,Sucrose ,Lifetech ECR8285 ,lcsh:Chemical technology ,01 natural sciences ,Catalysis ,lcsh:Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Xanthophyllomyces dendrorhous ,prebiotic nutrition ,fructosyltransferase ,lcsh:TP1-1185 ,Aspergillus terreus ,Physical and Theoretical Chemistry ,chemistry.chemical_classification ,Kluyveromyces lactis ,biology ,010405 organic chemistry ,digestive, oral, and skin physiology ,food and beverages ,Glycosidic bond ,Fructose ,biology.organism_classification ,Enzyme assay ,neofructo-oligosaccharides ,0104 chemical sciences ,030104 developmental biology ,Enzyme ,lcsh:QD1-999 ,chemistry ,Biochemistry ,Yield (chemistry) ,biology.protein - Abstract
Fructo-oligosaccharides (FOS) are prebiotic low-calorie sweeteners that are synthesized by the transfer of fructose units from sucrose by enzymes known as fructosyltransferases. If these enzymes generate &beta, (2,6) glycosidic bonds, the resulting oligosaccharides belong to the neoseries (neoFOS). Here, we characterized the properties of three different fructosyltransferases using a design of experiments approach based on response surface methodology with a D-optimal design. The reaction time, pH, temperature, and substrate concentration were used as parameters to predict three responses: The total enzyme activity, the concentration of neoFOS and the neoFOS yield relative to the initial concentration of sucrose. We also conducted immobilization studies to establish a cascade reaction for neoFOS production with two different fructosyltransferases, achieving a total FOS yield of 47.02 ±, 3.02%. The resulting FOS mixture included 53.07 ±, 1.66 mM neonystose (neo-GF3) and 20.8 ±, 1.91 mM neo-GF4.
- Published
- 2019
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