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Two-Step Production of Neofructo-Oligosaccharides Using Immobilized Heterologous Aspergillus terreus 1F-Fructosyltransferase Expressed in Kluyveromyces lactis and Native Xanthophyllomyces dendrorhous G6-Fructosyltransferase
- Source :
- Catalysts, Volume 9, Issue 8, Catalysts, Vol 9, Iss 8, p 673 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Fructo-oligosaccharides (FOS) are prebiotic low-calorie sweeteners that are synthesized by the transfer of fructose units from sucrose by enzymes known as fructosyltransferases. If these enzymes generate &beta<br />(2,6) glycosidic bonds, the resulting oligosaccharides belong to the neoseries (neoFOS). Here, we characterized the properties of three different fructosyltransferases using a design of experiments approach based on response surface methodology with a D-optimal design. The reaction time, pH, temperature, and substrate concentration were used as parameters to predict three responses: The total enzyme activity, the concentration of neoFOS and the neoFOS yield relative to the initial concentration of sucrose. We also conducted immobilization studies to establish a cascade reaction for neoFOS production with two different fructosyltransferases, achieving a total FOS yield of 47.02 &plusmn<br />3.02%. The resulting FOS mixture included 53.07 &plusmn<br />1.66 mM neonystose (neo-GF3) and 20.8 &plusmn<br />1.91 mM neo-GF4.
- Subjects :
- 0301 basic medicine
Sucrose
Lifetech ECR8285
lcsh:Chemical technology
01 natural sciences
Catalysis
lcsh:Chemistry
03 medical and health sciences
chemistry.chemical_compound
Xanthophyllomyces dendrorhous
prebiotic nutrition
fructosyltransferase
lcsh:TP1-1185
Aspergillus terreus
Physical and Theoretical Chemistry
chemistry.chemical_classification
Kluyveromyces lactis
biology
010405 organic chemistry
digestive, oral, and skin physiology
food and beverages
Glycosidic bond
Fructose
biology.organism_classification
Enzyme assay
neofructo-oligosaccharides
0104 chemical sciences
030104 developmental biology
Enzyme
lcsh:QD1-999
chemistry
Biochemistry
Yield (chemistry)
biology.protein
Subjects
Details
- Language :
- English
- ISSN :
- 20734344
- Database :
- OpenAIRE
- Journal :
- Catalysts
- Accession number :
- edsair.doi.dedup.....7af49d0cc805fd386b3ec04ecb2901a7
- Full Text :
- https://doi.org/10.3390/catal9080673