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Exogenous Classic Phytohormones Have Limited Regulatory Effects on Fructan and Primary Carbohydrate Metabolism in Perennial Ryegrass (Lolium perenne L.)
- Source :
- Frontiers in Plant Science, Frontiers in Plant Science, Frontiers, 2016, 6, ⟨10.3389/fpls.2015.01251⟩, Gasperl, A, Morvan-Bertrand, A, prud'homme, M-P, van der Graaff, E & Roitsch, T G 2016, ' Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass ( Lolium perenne L.) ', Frontiers in Plant Science, vol. 6, 1251 . https://doi.org/10.3389/fpls.2015.01251, Frontiers in Plant Science (6), . (2016), Frontiers in Plant Science, Vol 6 (2016)
- Publication Year :
- 2016
- Publisher :
- Frontiers Media S.A., 2016.
-
Abstract
- International audience; Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA), or kinetin (KIN). The most pronounced fast effects were a transient increase of I- I activities by AUX, KIN, ABA, and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Sucrose
fructan metabolism
enzymatic activity
Plant Science
lcsh:Plant culture
Biology
Carbohydrate metabolism
phytohormone
01 natural sciences
Lolium perenne
03 medical and health sciences
chemistry.chemical_compound
Fructan
Auxin
fructan exohydrolase
fructosyltransferase
perennial ryegrass
phytohormones
primary carbohydrate metabolism
Botany
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
lcsh:SB1-1110
Abscisic acid
Gibberellic acid
fructosyltransférase
ComputingMilieux_MISCELLANEOUS
Original Research
2. Zero hunger
chemistry.chemical_classification
Abiotic stress
activité enzymatique
métabolisme glucidique
fructane exohydrolase
food and beverages
biology.organism_classification
lolium pérenne
030104 developmental biology
chemistry
Biochemistry
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 1664462X
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science
- Accession number :
- edsair.doi.dedup.....7f87ee022aad207b338122f9562f03c2