101. Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
- Author
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Peter Olinga, Brendan W. Wren, Dirk Werling, Ivan Vokral, Elizabeth Atkins, Henny M. Martineau, Dominika Majorova, Jon Cuccui, and Dorenda Oosterhuis
- Subjects
0303 health sciences ,General Veterinary ,030306 microbiology ,Computer science ,Veterinary medicine ,Mini Review ,Host–pathogen interaction ,Tissue level ,Computational biology ,host-pathogen interaction ,Cellular level ,veterinary ,Host tissue ,Precision medicine ,Mini review ,03 medical and health sciences ,SF600-1100 ,Veterinary Science ,vaccinology ,Identification (biology) ,precision cut tissue slices ,immunology and infectious diseases ,030304 developmental biology - Abstract
The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches.
- Published
- 2021