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Identifying potential DNA methylation markers in early-stage colorectal Cancer
- Source :
- Genomics. 112(5)
- Publication Year :
- 2020
-
Abstract
- Colorectal cancer (CRC) is the second leading malignancy worldwide. Accurate screening is pivotal to early CRC detection, yet current screening modality involves invasive colonoscopy while non-invasive FIT tests have limited sensitivity. We applied a DNA methylation assay to identify biomarkers for early-stage CRC detection, risk stratification and precancerous lesion screening at tissue level. A model of biomarkers SFMBT2, ITGA4, THBD and ZNF304 showed 96.1% sensitivity and 87.0% specificity in CRC detection, with 100.0% sensitivity for advanced precancerous lesion and stage I CRC. Performances were further validated with TCGA data set, which showed a consistent AUC of 0.99 and exhibited specificity against other cancer types. KCNJ12, VAV3-AS1 and EVC were further identified for stage stratification (stage 0-I versus stage II-IV), with AUC of 0.87, 83.0% sensitivity and 71.2% specificity. Additionally, dual markers of NEUROD1 and FAM72C showed 83.2% sensitivity and 77.4% specificity in differing non-advanced precancerous lesions from inflammatory bowel diseases.
- Subjects :
- 0106 biological sciences
Oncology
Adult
Male
medicine.medical_specialty
Adolescent
Colorectal cancer
Colonoscopy
Biology
Malignancy
01 natural sciences
03 medical and health sciences
Young Adult
Internal medicine
Genetics
medicine
Biomarkers, Tumor
Humans
Child
030304 developmental biology
Aged
Neoplasm Staging
0303 health sciences
medicine.diagnostic_test
Tissue level
Inflammatory Bowel Diseases
Precancerous lesion
Methylation
DNA Methylation
Middle Aged
medicine.disease
DNA methylation
Disease Progression
Female
Colorectal Neoplasms
Precancerous Conditions
010606 plant biology & botany
Subjects
Details
- ISSN :
- 10898646
- Volume :
- 112
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....9cca800719c50523268cd77380863dc0