101. Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix
- Author
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Unne Stenram, Torkel Wadström, Peren H Karagin, and Åsa Ljungh
- Subjects
Adult ,DNA, Bacterial ,Male ,medicine.medical_specialty ,congenital, hereditary, and neonatal diseases and abnormalities ,Helicobacter pullorum ,Adolescent ,Brief Article ,Enterobacter ,Appendix ,Gastroenterology ,digestive system ,Polymerase Chain Reaction ,Microbiology ,Young Adult ,Internal medicine ,Helicobacter ,medicine ,otorhinolaryngologic diseases ,Bacteroides ,Humans ,Child ,Aged ,Meckel's diverticulum ,biology ,Infant ,General Medicine ,Helicobacter pylori ,Middle Aged ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Appendicitis ,digestive system diseases ,Meckel Diverticulum ,medicine.anatomical_structure ,surgical procedures, operative ,Child, Preschool ,Female ,Diverticulum - Abstract
AIM: To analyse the possible association of various Helicobacter species and certain common gut bacteria in patients with Meckel’s diverticulum and appendicitis. METHODS: A nested-polymerase chain reaction (PCR), specific to 16S rRNA of the Helicobacter genus, was performed on paraffin embedded samples, 50 with acute appendicitis, 50 normal appendixes, and 33 Meckel’s diverticulum with gastric heterotopia and/or ulcer. Helicobacter genus positive samples were sequenced for species identification. All samples were also analysed for certain gut bacteria by PCR. RESULTS: Helicobacter pullorum DNA was found in one out of 33 cases and Enterobacteria in two cases of Meckel’s diverticulum. Helicobacter pylori (H. pylori) was found in three, Enterobacter in 18, and Bacteroides in 19 out of 100 appendix samples by PCR. Enterococcus was not found in any MD or appendix samples. All H. pylori positive cases were from normal appendixes. CONCLUSION: Helicobacter is not an etiological agent in the pathogenesis of symptomatic Meckel’s diverticulum or in acute appendicitis.
- Published
- 2011