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Influence of activated charcoal, porcine gastric mucin and beta-cyclodextrin on the morphology and growth of intestinal and gastric Helicobacter spp
- Source :
- Microbiology; 148(3), pp 677-684 (2002)
- Publication Year :
- 2002
-
Abstract
- Bile-tolerant Helicobacter spp. are emerging human and animal pathogens. However, due to their fastidious nature, which requires nutrient-rich complex media to grow, infection with these bacteria may be underestimated. The accumulation of toxic metabolites in cultures may be one of the main obstacles for successful culture of these organisms. The present study examined various potential growth-enhancing substances for Helicobacter spp. and, furthermore, how they may affect spiral to coccoid conversion. Five Helicobacter spp. were cultured on agar and in broth media supplemented with activated charcoal, beta-cyclodextrin, or porcine gastric mucin. Growth was determined by estimating the numbers of colony-forming units and colony diameter, as well as bacterial cell mass. Coccoid transformation was estimated every 24 h by both Gram and acridine-orange staining. Activated charcoal was superior in supporting growth and increased cell mass on agar and in broth media. beta-Cyclodextrin delayed spiral to coccoid conversion by Helicobacter pylori and Helicobacter canis, whereas activated charcoal delayed the conversion to coccoid forms of Helicobacter hepaticus and Helicobacter bilis. The progression to coccoid forms by Helicobacter pullorum on agar media was not influenced by any growth supplement. The spiral to coccoid conversion was more rapid in broth media than on agar media. The growth enhancement observed is probably related to the capacity of activated charcoal to remove toxic compounds in culture media.
- Subjects :
- Swine
Colony Count
Colony Count, Microbial
coccoid forms
supplements
campylobacter-jejuni
Microbial
Agar
Helicobacter
Non-U.S. Gov't
Microscopy
biology
Stomach
ultrastructure
Intestines
cell-wall
Charcoal
Helicobacter hepaticus
Support
Human
Fastidious organism
Helicobacter bilis
food.ingredient
Helicobacter pullorum
Helicobacter pylori/*growth & development/isolation & purification/*ultrastructure
liver
Microbiology
Electron
Microbiology in the medical area
food
expression
Animals
Humans
liquid-media
Gastric Mucin
pylori
Helicobacter canis
Intestines/*microbiology
Bacteriological Techniques
Cyclodextrins
Helicobacter pylori
Animal
Gastric Mucins
biology.organism_classification
Culture Media
Stomach/*microbiology
Microscopy, Electron
Activated charcoal
coccoid conversion
sp-nov
Subjects
Details
- ISSN :
- 13500872 and 14652080
- Volume :
- 148
- Issue :
- Pt 3
- Database :
- OpenAIRE
- Journal :
- Microbiology (Reading, England)
- Accession number :
- edsair.doi.dedup.....323216c66e28682ef5e75823cbab593c