269 results on '"Neurospora growth & development"'
Search Results
52. Kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media.
- Author
-
Fiddy C and Trinci AP
- Subjects
- Agar, Aspergillus nidulans growth & development, Gels, Geotrichum metabolism, Kinetics, Mucor growth & development, Neurospora crassa metabolism, Silicon Dioxide, Culture Media, Geotrichum growth & development, Glucose metabolism, Mitosporic Fungi growth & development, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
The affinity (KS value) of Geotrichum candidum for glucose determined from chemostate cultures was ca. 1 mg/1. KS values for glucose was also estimated from the radial growth rates of colonies of G. candidum and Neurospora crassa grown on media solidified with agar or silica gel. An assessment is made of the use of colony radial growth rate to determine substrate affinities. The length of apical and intercalary hyphal comparte ments, internode length and the diameter of leading hyphaat the margin of colonies grown on solid media were all reduced at low glucose concentrations.
- Published
- 1975
- Full Text
- View/download PDF
53. [Derepression and conidiation in a Neurospora crassa mutant (acu-3) nonutilizing acetate].
- Author
-
Riatsch D, Peduzzi R, and Turian G
- Subjects
- Antigens, Fungal, Carbohydrate Metabolism, Electrophoresis, Polyacrylamide Gel, Immunoelectrophoresis, Neurospora crassa growth & development, Acetates metabolism, Malate Dehydrogenase metabolism, Mutation, Neurospora growth & development, Spores, Fungal growth & development
- Published
- 1974
54. Effects of antibiotics on the life cycle of Neurospora crassa.
- Author
-
Betina V, Janstová D, and Spisiaková J
- Subjects
- Cycloheximide pharmacology, Cyclopentanes pharmacology, Cytochalasins pharmacology, Dactinomycin pharmacology, Dose-Response Relationship, Drug, Fluorouracil pharmacology, Iodoacetates pharmacology, Mutation, Neurospora crassa drug effects, Phenylethyl Alcohol pharmacology, Photic Stimulation, Spores, Fungal drug effects, Spores, Fungal growth & development, Ultraviolet Rays, Xanthenes pharmacology, Antifungal Agents pharmacology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Some antibiotics and synthetic inhibitors affect, in several ways, the life cycle of Neurospora crassa (germination of conidia leads to myceliar growth leads to formation of conidia). Bikaverin, cyanein, scopathricin and phenethyl alcohol retard the germination of conidia, without inhibiting it completely. 5-Fluorouracil, ramihyphin A and zygosporin A (cytochalasin D) do not inhibit the germination. Bikaverin brings about a thickening of the hyphae of growing mycelium. Ramihyphin A, cyanein, scopathricin and zygosporin A stimulate the ramification of hyphae while 5-fluorouracil and phenethyl alcohol do not affect the myceliar morphology apart from their inhibitory effect on growth. Actinomycin D, 5-fluorouracil, cycloheximide, ramihyphin A and partially also sodium iodoacetate inhibit to a different degree the photoinduced formation of conidia. The inhibition by 5-fluorouracil is very conspicuous when the agent is present during the photoinduction but considerably weaker when it is applied 2 h after the photoinduction.
- Published
- 1975
- Full Text
- View/download PDF
55. Linear kalilo DNA is a Neurospora mitochondrial plasmid that integrates into the mitochondrial DNA.
- Author
-
Myers CJ, Griffiths AJ, and Bertrand H
- Subjects
- Blotting, Southern, Cell Nucleus ultrastructure, DNA, Mitochondrial isolation & purification, Female, Neurospora growth & development, Restriction Mapping, DNA, Mitochondrial genetics, Neurospora genetics, Plasmids genetics
- Abstract
The linear autonomous form of kalilo DNA (previously called AR-kalDNA) is shown to be resident within mitochondria rather than nuclei, as had been suggested by previous experiments. This form has been renamed mtAR-kalDNA, to signify its mitochondrial location. Experiments are described that illustrate the inheritance and somatic transmission patterns of the mitochondrial kalilo plasmid and the mitochondrial inserted form of kalilo DNA (mtIS-kalDNA). Progeny of a cross with a pre-senescent subculture as the female parent inherited mtAR-kalDNA only; mtIS-kalDNA was not transmitted sexually. During somatic propagation of the ascospore cultures, novel kalilo DNA inserts appeared and most of them persisted until death. We propose that these inserts originated from de novo integration of mtAR-kalDNA into the mitochondrial DNA. In two of the ascopore-derived series analyzed, the first inserts detected were seen only transiently and inserts appearing subsequent to the transient inserts were retained until death. We propose that these enduring inserts originated either from rearrangements of the transient inserts or from novel integration events, either from mtAR-kalDNA or from transposition of the transient inserts.
- Published
- 1989
- Full Text
- View/download PDF
56. Biochemical genetics of Neurospora crassa conidial germination.
- Author
-
Schmit JC and Brody S
- Subjects
- Amino Acids metabolism, Cell Wall ultrastructure, DNA biosynthesis, Fungal Proteins biosynthesis, Glutathione metabolism, Mutation, NAD metabolism, NADP metabolism, Neurospora crassa enzymology, Neurospora crassa metabolism, Neurospora crassa ultrastructure, RNA biosynthesis, Spores, Fungal enzymology, Spores, Fungal growth & development, Spores, Fungal metabolism, Spores, Fungal ultrastructure, Neurospora growth & development, Neurospora crassa growth & development
- Published
- 1976
- Full Text
- View/download PDF
57. Mutations specifically blocking differentiation of macroconidia Neurospora crassa.
- Author
-
Matsuyama SS, Nelson RE, and Siegel RW
- Subjects
- Cell Differentiation, Cell Division, Cell Movement, Cell Nucleus physiology, Time Factors, Mutation, Neurospora growth & development, Neurospora crassa growth & development, Spores, Fungal growth & development
- Published
- 1974
- Full Text
- View/download PDF
58. Neurospora crassa conidial germination: role of endogenous amino acid pools.
- Author
-
Schmit JC and Brody S
- Subjects
- Aminobutyrates metabolism, Aspartic Acid metabolism, Chromatography methods, Cycloheximide pharmacology, Flavin-Adenine Dinucleotide biosynthesis, Glutamates metabolism, NAD biosynthesis, Neurospora crassa metabolism, Spores, Fungal growth & development, Spores, Fungal metabolism, Water pharmacology, Amino Acids metabolism, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
The levels of the endogenous amino acid pools in conidia, germinating conidia, and mycelia of wild-type Neurospora crassa were measured. Three different chromatographic procedures employing the amino acid analyzer were used to identify and quantitatively measure 28 different ninhydrin-positive compounds. All of the common amino acids were detected in conidial extracts except proline, methionine, and cystine. The levels of these three amino acid pools were also very low in mycelia. During the first hour of germination in minimal medium, the levels of most of the free amino acid pools decreased. The pool of glutamic acid, the predominant free amino acid in conidia, decreased 70% during the first hour. Very little glutamic acid or any other amino acid was excreted into the medium. During the first 20 min of germination, the decrease in the glutamic acid pool was nearly equivalent to the increase in the aspartic acid pool. The aspartic acid and lambda-aminobutyric acid pools were the only amino acid pools that increased to maximum levels within the first 20 min of germination and then decreased. It is proposed that an important metabolic event that occurs during the early stages of conidial germination is the production of reduced pyridine nucleotides. The degradation of the large glutamic acid pool existing in the conidia (2.5% of the conidial dry weight) could produce these reduced coenzymes.
- Published
- 1975
- Full Text
- View/download PDF
59. Evidence for nuclear restriction of supersuppressor gene products in Neurospora heterokaryons.
- Author
-
Griffiths AJ
- Subjects
- Alleles, Genotype, Genes, Dominant, Hybrid Cells, Neurospora growth & development, Suppression, Genetic
- Abstract
It was demonstrated that the gene products of the three ssu loci cannot enter the transcription/translation system coded by the other component nucleus in a heterokaryon. In such a situation, dominance or recessiveness cannot be established, and simple dominance tests are meaningless.
- Published
- 1976
- Full Text
- View/download PDF
60. Ageing of Neurospora crassa. V. Lipid peroxidation and decay of respiratory enzymes in an inositol auxotroph.
- Author
-
Rana RS and Munkres KD
- Subjects
- Antioxidants pharmacology, Catechols pharmacology, Cytochrome Reductases metabolism, Electron Transport Complex IV metabolism, Hydrocortisone analogs & derivatives, Hydrocortisone pharmacology, Lipofuscin metabolism, Malondialdehyde metabolism, Mitochondria enzymology, Mitochondria metabolism, NADH, NADPH Oxidoreductases metabolism, Neurospora crassa enzymology, Neurospora crassa metabolism, Time Factors, Aging, Inositol metabolism, Lipid Metabolism, Neurospora growth & development, Neurospora crassa growth & development, Oxygen Consumption
- Published
- 1978
- Full Text
- View/download PDF
61. A study of the kinetics of hyphal extension and branch initiation of fungal mycelia.
- Author
-
Trinci AP
- Subjects
- Culture Media, Geotrichosis, Kinetics, Neurospora crassa growth & development, Penicillium chrysogenum growth & development, Photography, Time Factors, Aspergillus nidulans growth & development, Mitosporic Fungi growth & development, Mucor growth & development, Neurospora growth & development, Penicillium growth & development
- Published
- 1974
- Full Text
- View/download PDF
62. Inversions and recombinations in mitochondrial DNA of the (SG-1) cytoplasmic mutant in two Neurospora species.
- Author
-
Infanger A and Bertrand H
- Subjects
- Chromosome Inversion, Cytochromes analysis, DNA Restriction Enzymes, DNA, Fungal genetics, Gene Conversion, Mitochondria physiology, Mutation, Neurospora growth & development, Neurospora crassa genetics, Neurospora crassa growth & development, Recombination, Genetic, Species Specificity, DNA, Mitochondrial genetics, Neurospora genetics
- Abstract
The mitochondrial DNAs of [SG-1] cytoplasmically-mutant and wild-type strains of Neurospora crassa and Neurospora sitophila were examined by comparative restriction endonuclease analyses. The mtDNA of N. sitophila wild type of Whitehouse differs from type II mtDNA of N. crassa by insertions of 3.3 kb in EcoRI-9, and 1.2 kb in EcoRI-3, and a deletion of 1.1 kb in EcoRI-5. These DNA heteromorphisms provided convenient markers for tracing N. crassa [SG-1] mtDNA during and after its transfer into N. sitophila. The [SG-1] cytoplasmic mutant in both N. crassa and N. sitophila has a distinctive inversion that connects the fragment EcoRI-4 with HindIII-10a. The [SG-1] mtDNA from N. crassa remained essentially intact after it was transferred by crosses into N. sitophila. In each species, a unique second inversion occurred in the [SG-1] mtDNA after the transfer was made. In N. sitophila, polar recombination in heteroplasmons between [SG-1] and wild-type preferentially yields strains with mtDNAs that contain the maximum possible number of insertions in the cob and co-1 loci of the EcoRI-3 region of the mitochondrial chromosome.
- Published
- 1986
- Full Text
- View/download PDF
63. Implications of some genetic control mechanisms in Neurospora.
- Author
-
Metzenberg RL
- Subjects
- Amino Acids metabolism, DNA, Fungal metabolism, Mutation, Neurospora growth & development, Neurospora metabolism, Neurospora physiology, Nitrogen metabolism, Peptide Hydrolases metabolism, Phosphoric Monoester Hydrolases metabolism, Quinic Acid metabolism, Neurospora genetics
- Published
- 1979
- Full Text
- View/download PDF
64. Phase-specific genes for macroconidiation in Neurospora crassa.
- Author
-
Selitrennikoff CP, Nelson RE, and Siegel RW
- Subjects
- Autolysis, Cell Wall, Chromosome Mapping, Crosses, Genetic, Genetic Complementation Test, Heterozygote, Homozygote, Microscopy, Electron, Scanning, Morphogenesis, Phenotype, Spores, Fungal growth & development, Genes, Mutation, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Two new mutant genes in Neurospora crassa prevent the formation of free macroconidia from proconidial chains. These genes, called conidial separation-1 and conidial separation-2, are phase-specific, playing no role in either the sexual life cycle or other aspects of the asexual life cycle. A cell-wall-associated autolytic activity was found to increase in wild-type cultures at the time of active formation of free conidia from proconidial chains; no such increase was detected in mutant cultures. It appears that the products of these genes are both essential for and unique to macroconidiation.
- Published
- 1974
- Full Text
- View/download PDF
65. [Growth of yeasts on a nutrient medium with deuterated compounds].
- Author
-
Lazareva AV, Shishkanova NV, and Lozinov AB
- Subjects
- Amino Acids metabolism, Ammonium Sulfate metabolism, Candida radiation effects, Cryptococcus metabolism, Cryptococcus radiation effects, Cryptococcus neoformans growth & development, Cryptococcus neoformans radiation effects, Culture Media, Glucose metabolism, Neurospora crassa radiation effects, Time Factors, Candida growth & development, Cryptococcus growth & development, Deuterium, Neurospora growth & development, Neurospora crassa growth & development, Radiation Effects
- Published
- 1974
66. Ethylene glycol-induced alteration of conidial germination in Neurospora crassa.
- Author
-
Bates WK and Wilson JF
- Subjects
- Cell Fractionation, Cell Nucleus, Culture Media, Cytochrome c Group analysis, Cytoplasm, Glycerol pharmacology, Microscopy, Phase-Contrast, Mitochondria analysis, Neurospora drug effects, Neurospora crassa analysis, Neurospora crassa drug effects, Neurospora crassa growth & development, Osmosis, Spores, Fungal drug effects, Time Factors, Glycols pharmacology, Neurospora growth & development, Spores, Fungal growth & development
- Abstract
In nutrient medium containing 3.22 M ethylene glycol or glycerol, conidia of Neurospora crassa grow as single cells, without forming the germ tubes characteristic of normal morphological germination. Ethylene glycol is more effective than glycerol in producing this response. After growth in ethylene glycol medium for a suitable time, the cells are easily disrupted by an abrupt decrease in osmotic pressure. Osmotic disruption yields intact nuclei and mitochondria, although mitochondrial fractions obtained in this way show significantly reduced concentrations of cytochromes c + c(1), as compared to those observed for comparable fractions obtained from vegetative hyphae. Cell cultures gradually adapted to lower concentrations of the glycol show a much higher degree of synchrony in the formation of germ tubes than do untreated conidia.
- Published
- 1974
- Full Text
- View/download PDF
67. Assay of rate of aging of conidia of Neurospora crassa.
- Author
-
Munkres KD and Furtek CA
- Subjects
- Cell Division, Crosses, Genetic, Culture Media, Kinetics, Microbiological Techniques, Mutation, Species Specificity, Neurospora growth & development, Neurospora crassa growth & development
- Published
- 1984
- Full Text
- View/download PDF
68. Polysomes, ribonucleic acid, and protein synthesis during germination of Neurospora crassa conidia.
- Author
-
Mirkes PE
- Subjects
- Adenine metabolism, Carbon Radioisotopes, Centrifugation, Density Gradient, Culture Media, Fungal Proteins biosynthesis, Leucine metabolism, Magnesium pharmacology, Micropore Filters, Neurospora crassa cytology, Neurospora crassa growth & development, Neurospora crassa metabolism, Puromycin pharmacology, RNA, Messenger biosynthesis, RNA, Ribosomal biosynthesis, Ribonucleases pharmacology, Spectrophotometry, Spores, Fungal cytology, Spores, Fungal growth & development, Spores, Fungal metabolism, Tritium, Uracil metabolism, Water, Neurospora growth & development, Polyribosomes metabolism, RNA biosynthesis
- Abstract
The level of polysomes in ungerminated conidia of Neurospora crassa depends on the method used to collect spores. Spores harvested and exposed to hydration contain 30% of their ribosomes as polysomes, whereas those not exposed to hydration contain only 3% of their ribosomes as polysomes. During the germination process, the percentage of the ribosomes which sediment as polysomes increases rapidly to a level of approximately 75% during the first 15 to 30 min of germination. This rapid increase has been shown to require a carbon source. During the first 30 min of germination, spores synthesize ribosomal ribonucleic acid (RNA) and heterogeneously sedimenting RNA, i.e., presumptive messenger RNA.
- Published
- 1974
- Full Text
- View/download PDF
69. Ethanol and carbon-source starvation enhance the accumulation of HSP80 in Neurospora crassa.
- Author
-
Roychowdhury HS and Kapoor M
- Subjects
- Carbohydrate Metabolism, Molecular Weight, Neurospora crassa drug effects, Neurospora crassa metabolism, Ethanol pharmacology, Heat-Shock Proteins biosynthesis, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
In Neurospora crassa, heat shock results in the induction of 9 to 11 heat shock proteins (HSP), of which HSP80 is the most abundant and the first to be synthesized. The induction of HSP80 was investigated during normal growth (2% sucrose) and under sucrose starvation. Transfer of mycelium to a medium supplemented with ethanol stimulated the synthesis of HSP80, even at the normal growth temperature of 28 degrees C. It was also synthesized under carbon starvation conditions, where the medium was supplemented with 0.02% sucrose, 0.3% acetate, 0.2% lactate, or ethanol. A 30-35 kilodalton polypeptide was induced by heat shock in carbon-sufficient media, but in 0.02% sucrose and 0.3% acetate containing media it was synthesized at normal temperatures. While the overall heat shock response remained unaltered in these cultures, the abundance of HSP90 and HSP70, relative to HSP80, was greater. HSP80 appears to be controlled by carbon-catabolite repression as well as heat shock. Another high molecular mass protein (tentatively designated alc'80') was observed to be induced by heat shock, provided carbon starvation conditions prevailed concurrently.
- Published
- 1988
- Full Text
- View/download PDF
70. Dormancy in spores of fungi.
- Author
-
Cochrane VW
- Subjects
- Ascomycota growth & development, Basidiomycota growth & development, Cell Survival, Fungi growth & development, Neurospora growth & development, Adaptation, Biological, Spores, Fungal growth & development
- Published
- 1974
71. A mutant affecting meiosis in Neurospora.
- Author
-
Smith DA
- Subjects
- Aneuploidy, Chromatids, Crosses, Genetic, Crossing Over, Genetic, Genes, Recessive, Genetic Linkage, Homozygote, Neurospora ultrastructure, Pigmentation, Spores, Fungal growth & development, Meiosis, Mutation, Neurospora growth & development
- Abstract
Many mutants affecting meiosis increase the occurrence of aneuploid meiotic products. In Neurospora, mutants of this type cause ascospore abortion which is reflected by an increase in the proportion of ascospores failing to develop black pigment. The usefulness of the criterion white-ascospore-production as a signal for the presence of a mutant affecting meiosis is demonstrated by the recovery of several such mutants. One of these is mei-1 (meiotic-1), a recessive mutant on linkage group IV. Crosses homozygous for mei-1 produce 90% white ascospores (vs. 5% in wild-type crosses). Viable ascospores, invariably black, are always disomic for one or more linkage groups; the chromatids assorted into viable ascospores do not engage in crossing over in meiosis. The distribution of viable ascospores in individual asci suggests that all meioses are defective in the first meiotic division, and that most meioses are defective in both divisions.
- Published
- 1975
- Full Text
- View/download PDF
72. Development of amino acid uptake activity in Neurospora.
- Author
-
Railey RM and Kinsey JA
- Subjects
- Arginine metabolism, Glucose metabolism, Hydrogen-Ion Concentration, Membrane Transport Proteins metabolism, Mutation, Neurospora enzymology, Neurospora growth & development, Phenylalanine metabolism, Quaternary Ammonium Compounds metabolism, Spores, Fungal enzymology, Spores, Fungal growth & development, Spores, Fungal metabolism, Tryptophan analogs & derivatives, Tryptophan metabolism, Amino Acids metabolism, Neurospora metabolism
- Abstract
During the germination and growth of Neurospora conidia, amino acid permease systems I (neutral) and II (general) increase in specific activity. System III (basic) decreases in specific activity with the onset of germination. System I shows two peaks of activity during the logarithmic phase of growth. One peak occurs at 6 h, the other at 12 h of growth. Both peaks are abolished in the mtr mutant. Both peaks have a Km for phenylalanine of 40 muM. The peaks of system I activity appear to correlate with morphological changes.
- Published
- 1976
- Full Text
- View/download PDF
73. Inhibition of germination and sporulation (conidiation) in Neurospora sitophila Shear and Dodge by carbon dioxide.
- Author
-
Ramirez C
- Subjects
- Agar, Culture Media, Neurospora metabolism, Carbon Dioxide biosynthesis, Neurospora growth & development, Spores, Fungal growth & development
- Published
- 1974
- Full Text
- View/download PDF
74. Effects of carbon sources on the levels of fructose 1,6-biphosphathase in Neurospora crassa.
- Author
-
Zarraga AM and Hubert E
- Subjects
- Fructose-Bisphosphatase antagonists & inhibitors, Neurospora crassa enzymology, Time Factors, Carbon metabolism, Fructose-Bisphosphatase analysis, Neurospora growth & development, Neurospora crassa growth & development
- Published
- 1978
75. Genetically determined conidial longevity is positively correlated with superoxide dismutase, catalase, glutathione peroxidase, cytochrome c peroxidase, and ascorbate free radical reductase activities in Neurospora crassa.
- Author
-
Munkres KD, Rana RS, and Goldstein E
- Subjects
- Isoenzymes metabolism, Mutation, Neurospora crassa enzymology, Neurospora crassa genetics, Time Factors, Catalase metabolism, Cytochrome-c Peroxidase metabolism, Glutathione Peroxidase metabolism, NADH, NADPH Oxidoreductases metabolism, Neurospora growth & development, Neurospora crassa growth & development, Peroxidases metabolism, Superoxide Dismutase metabolism
- Abstract
Aging of post-mitotic cells, the conidia, of Neurospora crassa is defined as the time-dependent loss of viability under a constant laboratory environment which probably resembles the organism's tropical habitat; namely, at 30 degrees C, 85-100% relative humidity under white light. Median lifespan is defined as the age at which survival of a conidial population has declined to 50% of that of a fully viable population at birth. A collection of short (age-) and long-lived (age+) mutants were previously selected from the wild-type whose median lifespan is 22 days. Thus, five groups of strains with distinct lifespans of 7, 22, 36, 50 and 60 days were defined. The purposes of the present investigation were to determine if the activities of anti-oxygenic enzymes are correlated with lifespan and to elucidate the function of the cellular longevity determinant genes. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) were highly-correlated with lifespan; whereas glutathione reductase and non-specific peroxidase activities were not correlated. The short-lived mutants were also deficient in cytochrome c peroxidase (CPX) and ascorbate free radical reductase (AFR), but not deficient in dehydroascorbate reductase. (These latter three enzymes were not examined in age+ mutants.) By isoelectric focusing analysis, the deficiencies of SOD, CAT, and GPX activities of age- mutants were defined in terms of specific isozymes. The mutants were specifically deficient in a cyanide-resistant mitochondrial isozyme of SOD. Sixteen age- genes, called the age-1 complex, were previously mapped on one arm of the seven chromosomes. On the basis of mapping and complementation data, it was inferred that the genes are spatially and functionally redundant. The hypothesis of functional redundancy is also supported by the enzyme data. Of seven mutants examined, representing seven of the age- genes, all were deficient in SOD, CAT and CPX, and six were deficient in AFR. Of four mutants examined, representing four of the genes, all were deficient in GPX. The results indicate a molecular basis for the previously observed photosensitivity of the mutants.(ABSTRACT TRUNCATED AT 400 WORDS)
- Published
- 1984
- Full Text
- View/download PDF
76. Cellular events in growth and germination of giant conidia of Neurospora crassa.
- Author
-
Levi M, Sacco G, and Trezzi F
- Subjects
- DNA, Fungal biosynthesis, Fungal Proteins biosynthesis, Neurospora crassa drug effects, Spores, Fungal growth & development, Ethylene Glycols pharmacology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
During growth of conidia in 3.22 M ethylene glycol the increase in the number of the nuclei is proportional to the increase in volume only in the phase of maximum growth rate and is lower in the preceding and in the following periods of growth. DNA synthesis similarly initiates later and decreases faster than protein synthesis. The dilution of ethylene glycol is followed by the germination of giant conidia, which is characterized by the absence of a lag phase, a high degree of synchrony and the formation of more than one germ tube per conidium. The number of germ tubes is dependent on the volume reached by conidia at the end of the treatment and does not increase with time. The resuming of DNA synthesis after germination is preceded by a sharp increase in protein synthesis and the division of almost half of the nuclei and shows a synchronized pattern. Results are discussed in the light of models of growth proposed for eukaryotic cells.
- Published
- 1980
77. Changes in nucleotide pools during conidial germination in Neurospora crassa.
- Author
-
Sabina RL, Dalke P, Hanks AR, Magill JM, and Magill CW
- Subjects
- Chromatography, High Pressure Liquid, Histidine pharmacology, Neurospora crassa drug effects, Neurospora growth & development, Neurospora crassa growth & development, Nucleotides metabolism
- Published
- 1981
- Full Text
- View/download PDF
78. Uptake, intracellular binding, and excretion of polyamines during growth of Neurospora crassa.
- Author
-
Davis RH and Ristow JL
- Subjects
- Arginase metabolism, Arginine metabolism, Biological Transport, Cell Membrane metabolism, Cell Membrane Permeability, Mutation, Neurospora crassa metabolism, Ornithine metabolism, Ornithine Decarboxylase metabolism, Putrescine metabolism, Spermidine metabolism, Spermidine Synthase metabolism, Neurospora growth & development, Neurospora crassa growth & development, Polyamines metabolism
- Abstract
In Neurospora crassa mycelia, the amounts of the main polyamines, putrescine and spermidine, are approximately 0.8 and 18 nmol/mg, dry weight. We wished to know what determines these pool sizes. In the growth medium, externally added polyamines enter cells largely by a nonsaturable, diffusional system. In a mutant unable to polyamines, internal and external spermidine appear to equilibrate across the cell membrane during growth. However, this was true only after an intracellular "sink," with a capacity equal to the amount of spermidine found in wild-type cells, had been saturated. We speculate that internal anionic binding sites, detectable in permeabilized cells, sequester virtually all of the spermidine normally found in exponentially growing N. crassa. Further evidence for this view was that in mature, stationary cultures, excess spermidine is excreted. Putrescine is also excreted if its concentration in the cell is abnormally high. The control of pool size by intracellular binding and excretion may be an advantage in this pathway, because feedback inhibition does not prevail, enzyme regulation is by comparison slow, and excessive polyamines are toxic.
- Published
- 1989
- Full Text
- View/download PDF
79. Effect of temperature and temperature shifts on growth and branching of a wild type and a temperature sensitive colonial mutant (Cot 1) of Neurospora crassa.
- Author
-
Steele GC and Trinci AP
- Subjects
- Species Specificity, Mutation, Neurospora growth & development, Neurospora crassa growth & development, Temperature
- Abstract
Growth of a temperature sensitive colonial mutant (cot 1) of Neurospora crassa was compared with a wild type strain. The hyphal growth unit (the ratio between mycelial length and number of branches) of the wild type was not appreciably altered by temperature and there was a direct relationship between the specific growth rate (alpha) of these mycelia and their mean hyphal extension rate (E). The specific growth rate of cot 1 increased by about the same relative amount as the wild type between 15 degrees and 30 degrees C. Cot 1 grew and branched normally at 15 degrees and 25 degrees C but at 30 degrees C the hyphal growth unit and mean hyphal extension rate of the mutant mycelia were reduced. Thus, between 15-30 degrees C the ratio, E/alpha was constant for the wild type but not for cot 1. The effect of temperature and temperature shifts on extension zone length (2), extension zone expansion time (2t) and branching of leading hyphae of mature colonies were also studied. It is suggested that branching is governed by a mechanism which regulates the linear growth rate of hyphae; the cot 1 mutation may have a direct effect on wall extension or affect linear growth rate indirectly due to an influence on the transport of precursors to the tip.
- Published
- 1977
- Full Text
- View/download PDF
80. Circadian rhythms in Neurospora crassa: membrane composition of a mutant defective in temperature compensation.
- Author
-
Coté GG and Brody S
- Subjects
- Cell Membrane analysis, Fatty Acids analysis, Neurospora crassa genetics, Temperature, Circadian Rhythm, Membrane Lipids analysis, Mutation, Neurospora growth & development, Neurospora crassa growth & development, Phospholipids analysis
- Abstract
The cel mutant of Neurospora, partially blocked in fatty acid synthesis and lacking temperature compensation of its circadian rhythm below 22 degrees C, had a phospholipid fatty acid composition in liquid shaker culture distinctly different from that of a cel+ control strain. During growth, cel+ exhibited a reproducible increase in its linoleic acid level from about 32 to a plateau at 63 mol%, and a corresponding decrease in its linolenic acid level from about 40 to a plateau at 10 mol%. The level of palmitic acid was constant at 19 mol%. In the cel strain, the linoleic acid level was constant at 54 mol% while the palmitic acid level increased from about 12 to about 23 mol%. Supplementation with palmitic or linoleic acids altered the patterns of fatty acid composition of cel, but did not affect the pattern of cel+. Altered fatty acid composition cosegregated with the cel marker. The mitochondrial phospholipids of cel in liquid culture also had abnormal fatty acid composition, as did the whole mycelial phospholipids on solid medium. These results are consistent with the involvement of membrane homeostasis in the temperature compensation of circadian rhythms.
- Published
- 1987
- Full Text
- View/download PDF
81. Characterization of glycerol nonutilizing and protoperithecial mutants of Neurospora.
- Author
-
Viswanath-Reddy M, Bennett SN, and Howe HB Jr
- Subjects
- Chromosome Mapping, Genetic Complementation Test, Genetic Linkage, Mannitol metabolism, Neurospora growth & development, Sorbitol metabolism, Xylitol metabolism, Glycerol metabolism, Mutation, Neurospora metabolism
- Abstract
Mutants defective in polyol metabolism and/or in protoperithecial development were selected in Neurospora tetrasperma, a species in which protoperithecial development occurs at nonpermissively high temperature if certain polyols are used in lieu of sucrose as carbon source. Mutants selected for nonutilization of one of the four polyols tested, glycerol, mannitol, sorbitol, or xylitol, were usually found to be nonutilizers of the other three polyols as well. Mutants blocked at various stages of protoperithecial development complemented pairwise to produce more advanced developmental stages, usually mature protoperithecia and, when of opposite mating type, mature perithecia. About one-third of the mutants manifested both polyol auxotrophy and defective protoperithecial development upon initial isolation, but protoperithecial defectiveness in such mutants usually showed erratic segregation in crosses and/or instability to repeated vegetative transfer, whereas polyol auxotrophy usually did not and was, therefore, studied further. Two glycerol nonutilizing strains were introgressed into N. crassa to facilitate genetic analysis. One, glp-4, lacked both inducible and constitutive glycerol kinase and mapped to linkage group VI, between ad-1 and rib-1; the other, glp-5, lacked glyceraldehyde kinase and mapped to linkage group I, proximal to ad-9. Another mutant, gly-u(234), has been reported by other investigators to lack inducible glycerol kinase but to map to linkage group I, distal to ad-9.
- Published
- 1977
- Full Text
- View/download PDF
82. Preparation of functional group analogs of unsaturated fatty acids and their effects on the circadian rhythm of a fatty-acid-deficient mutant of Neurospora crassa.
- Author
-
Mattern DL
- Subjects
- Chemical Phenomena, Chemistry, Fatty Acids metabolism, Fatty Acids, Unsaturated chemical synthesis, Mutation, Neurospora crassa genetics, Neurospora crassa metabolism, Circadian Rhythm, Fatty Acids, Unsaturated pharmacology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Functional group analogs of oleic, linoleic and linolenic acids were prepared by coverting their double bonds to dibromo, cyclopropyl, epoxy, methoxy, and, in the case of oleic acid, hydroxy groups. These compounds were supplemented to the bd csp cel strain of the mold Neurospora crassa. The cel mutation confers a partial requirement for saturated fatty acids and, also, perturbs the circadian rhythm of spore formation. For example, the period of bd csp cel's rhythm is dramatically lengthened upon supplementation by natural cis-unsaturated fatty acids. Of the analogs tested, only the monoepoxy, monomethoxy, dibromo, and hexabromo stearic acids gave significant period lengthening. Other analogs, which should have comparable abilities to disrupt lipid bilayer packing, gave no rhythm effect. Further, the inactive di- and tri-methoxystearic acid analogs were incorporated to a greater extent than the active mono-methoxystearic acid. The results do not, therefore, support a direct alteration in membrane "fluidity' as the mode of action of the period-lengthening fatty acids.
- Published
- 1985
- Full Text
- View/download PDF
83. Liberation of protoplasts from mycelium of Neurospora crassa by means of enzymes obtained from autolyzed cultures of this fungus.
- Author
-
Reyes F and Lahoz R
- Subjects
- Neurospora crassa enzymology, Osmotic Pressure, Temperature, Time Factors, Autolysis, Neurospora growth & development, Neurospora crassa growth & development, Protoplasts
- Abstract
Spherical protoplast-like structures were released from Neuropsora crassa mycelia under the influence of cell-wall-lytic enzymes obtained from autolyzed cultures of this fungus, in 50 mM borate-citrate-phosphate buffer, pH 5.5 with 0.5-0.8 M mannitol for appropriate osmotic pressure. The protoplasts retain their morphological aspect for several days at 4 C, or for a longer time when they are washed by centrifugation and incubated with 0.8 M mannitol. The free protoplasts are sensitive to osmotic shock and lyse immediately when placed in distilled water; the vacuoles and other cytoplasmatic material remain intact for some time.
- Published
- 1976
- Full Text
- View/download PDF
84. Nuclear suppressors of the (poky) cytoplasmic mutant in Neurospora crassa. II. Mitochondrial cytochrome systems.
- Author
-
Bertrand H and Kohout J
- Subjects
- Alleles, Mutation, Ribosomes metabolism, Cytochromes metabolism, Mitochondria metabolism, Neurospora growth & development, Neurospora crassa growth & development, Suppression, Genetic
- Abstract
The mitochondrial cytochrome aa3 and b deficiencies of the [poky] cytoplasmic mutant of Neurospora crassa are partially suppressed by mutant alleles of any one of six nuclear genes, namely sup-1, sup-3, sup-4, sup-5, sup-10 and sup-14. The suppressor-induced increases in the concentration of both cytochromes are detected in the mitochondria from exponentially growing [poky] cultures, and, thus, are clearly distinguishable from the age-dependent changes in the cytochrome system that occur in cultures that approach, or have reached, the stationary phase of growth. The relative amounts of mitochondrial cytochromes aa3 and b show a direct correlation with the relative efficiency of the various sup genes as suppressors of the slow-growth phenotype of [poky]. Since [poky] is defective in mitochondrial protein synthesis due to a lack of 30 S mitochondrial ribosomal subunits, it is proposed that the six suppressors promote the assembly of functional mitochondrial ribosomes.
- Published
- 1977
- Full Text
- View/download PDF
85. Interaction of galactosaminoglycan with Neurospora conidia.
- Author
-
Glasgow JE and Reissig JL
- Subjects
- Absorption, Binding Sites drug effects, Carbon Radioisotopes, Galactosamine metabolism, Glucose metabolism, Glycosaminoglycans metabolism, Hydrogen-Ion Concentration, Kinetics, Neurospora crassa drug effects, Proline metabolism, Temperature, Time Factors, Galactosamine pharmacology, Neurospora growth & development, Neurospora crassa growth & development, Polysaccharides pharmacology
- Abstract
The inactivation of Neurospora crassa conidia by galactosaminoglycan isolated from cultures of this organism was followed by measuring colony-forming ability and ability to take up radiolabeled metabolites. When kinetic data on the loss of transport function and on killing were analyzed by use of target theory, it appeared that few "hits" are required for inactivation. However, studies with radio-labeled galactosaminoglycan mucopolysaccharides showed that cells receiving a single lethal hit have approximately 10(5) galactosaminoglycan molecules bound to them.
- Published
- 1974
- Full Text
- View/download PDF
86. Ageing of Neurospora crassa. III. Induction of cellular death and clonal senescence of an inositol-less mutant by inositol starvation and the protective effect of dietary antioxidants.
- Author
-
Munkres KD
- Subjects
- Benzyl Alcohols pharmacology, Catechols pharmacology, Mutation, Neurospora crassa metabolism, Vitamin E pharmacology, Antioxidants pharmacology, Inositol metabolism, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Clonal growth rate and cellular viability of an inositol-less mutant of Neurospora crassa decline rapidly during deprivation of dietary inositol. Dietary antioxidants, either nordihydroguaiaretic acid, vitamin E or 3,5-ditert.-butyl-4-hydroxybenzyl alcohol, protected cells and clones of the mutant from death and senescence. Membrane deterioration with associated lipid autoxidation and consequent deleterious free radical reactions appear to be, in part at least, the causes of the mutant's death and senescence.
- Published
- 1976
- Full Text
- View/download PDF
87. Ageing of Neurospora crassa. VI. Cytochemical and cytological correlates of senescence in three model systems.
- Author
-
Rana RS and Munkres KD
- Subjects
- Antioxidants pharmacology, Cell Membrane physiology, Culture Media analysis, Electron Transport Complex IV metabolism, Mitochondria enzymology, Neurospora crassa enzymology, Osmotic Pressure, Proteins analysis, Time Factors, Aging, Neurospora growth & development, Neurospora crassa growth & development
- Published
- 1978
- Full Text
- View/download PDF
88. Analysis of pattern formation in Neurospora perithecial development using genetic mosaics.
- Author
-
Johnson TE
- Subjects
- Animals, Models, Biological, Mutation, Sex, Spores, Fungal, Mosaicism, Neurospora growth & development
- Published
- 1976
- Full Text
- View/download PDF
89. Soil fungistasis: role of the microbial nutrient sink and of fungistatic substances in two soils.
- Author
-
Bristow PR and Lockwood JL
- Subjects
- Ascomycota growth & development, Ascomycota metabolism, Carbon Dioxide biosynthesis, Glucose metabolism, Mitosporic Fungi growth & development, Mitosporic Fungi metabolism, Neurospora growth & development, Neurospora metabolism, Spores, Fungal growth & development, Spores, Fungal metabolism, Water, Antifungal Agents pharmacology, Fungi growth & development, Soil, Soil Microbiology
- Abstract
Sensitivity of conidia of Cochliobolus victoriae to fungistasis decreased markedly following incubation on moist sand for at least 1 h. Germination was greater on Conover loam or on sand being leached with water than on an alkaline clay loam soil known to produce a volatile fungistatic substance. Evolution of 14CO2 began within 3 min after [14C]glucose was applied to the soils; the rate of 14CO2 evolution was faster with Conover loam. Germination of Thielaviopsis basicola conidia per unit of glucose remaining in agar discs initially containing 0-1% glucose, was lower for discs incubated on the clay loam soil than on Conover loam, and was greatest on a bed of sand undergoing aqueous leaching. Germination of ascospores of Neurospora tetrasperma and conidia of C. victoriae was suppressed on discs of washed, Purified Agar or polyacrylamide gel incubated on or over the clay loam soil, but no suppression resulted when discs were incubated on Conover loam. Extensive aeration of either soil did not remove its fungistatic effect. Fungistasis in Conover loam appears to be caused primarily by nutrient deprivation, whereas volatile fungistatic substances may play a major role in the clay loam soil.
- Published
- 1975
- Full Text
- View/download PDF
90. Cell-biology of ageing. III. Malondialdehyde as an index of free radical reactions in the early senescent mutants of Neurospora crassa and study of the effect of free radical scavengers on malondialdehyde contents.
- Author
-
Islam MS and Nessa A
- Subjects
- Ascorbic Acid pharmacology, Neurospora crassa drug effects, Neurospora crassa genetics, Selenious Acid, Selenium pharmacology, Time Factors, Vitamin E pharmacology, Free Radicals, Malonates metabolism, Malondialdehyde metabolism, Mutation, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Malondialdehyde - a product of lipid peroxidation due to free radical reaction was estimated in the culture filtrates of early senescent mutants of Neurospora crassa and the effects of vitamin E, vitamin C and Sodium selenite (free radical scavengers) in malondialdehyde contents were studied. From the results obtained, it could not be established that increased free radical reaction was the sole factor for the early senescence of all the mutants; and the free radical scavengers had very little effect on the malondialdehyde content of the selected early senescent mutants.
- Published
- 1984
- Full Text
- View/download PDF
91. Ultrastructural study of microcyclic macroconidiation in Neurospora crassa.
- Author
-
That TC and Turian G
- Subjects
- Cell Nucleus ultrastructure, Cell Wall ultrastructure, Neurospora crassa ultrastructure, Spores, Fungal growth & development, Spores, Fungal ultrastructure, Hot Temperature, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Heat-shock of macroconidia of Neurospora crassa at 46 degrees C followed by shift-down to 25 degrees C determines premature conidiogenesis. The nuclei and cytoplasmof heat-treated, swollen conidia contain spots of a dense material especially concentrated around the nucleolus in short time treated ones. In the first proconidium apically budding on the enlarged tip of the premature conidiophore, small vesicles are later seen lining the initially simple septum separating the proconidial units into conidia. The doubling of this interconidial septum is surface viewn as a thick annulus. Disarticulation of the conidial untis intervenes along a septal furrow of electroluscent material. Interconidial continuity through the septal proes is transiently insured by a connective which is ruptured for final liberation of the conidia.
- Published
- 1978
- Full Text
- View/download PDF
92. Biochemical genetics of morphogenesis in Neurospora.
- Author
-
Scott WA
- Subjects
- Cell Wall ultrastructure, Circadian Rhythm, Glucose-6-Phosphate Isomerase biosynthesis, Glucosephosphate Dehydrogenase biosynthesis, Morphogenesis, Mutation, Neurospora enzymology, Neurospora ultrastructure, Phosphoglucomutase biosynthesis, Phosphogluconate Dehydrogenase biosynthesis, Spores, Fungal growth & development, Neurospora growth & development
- Published
- 1976
- Full Text
- View/download PDF
93. Circadian rhythms in Neurospora crassa: effects of saturated fatty acids.
- Author
-
Mattern D and Brody S
- Subjects
- Neurospora crassa drug effects, Spores, Fungal drug effects, Spores, Fungal physiology, Structure-Activity Relationship, Circadian Rhythm drug effects, Fatty Acids pharmacology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
To assess their effects on the conidiation rhythm in Neurospora, 14 saturated fatty acids from 6 to 24 carbons long were used to supplement the bd csp and bd csp cel strains. Both strains express a circadian spore-forming rhythm when grown on solid media; the cel mutation confers a partial fatty acid requirement. Fatty acid supplements from 8 to 13 carbons long lengthened the free-running period of bd csp cel compared with the control value of 21 h; the maximal effect (33 h) was obtained with nonanoic acid (9:0) at a concentration of 5 x 10(-4) M. In contrast, the period of bd csp remained unchanged under all experimental conditions. The short-chain fatty acids (<14 carbons) reduced the rate of advance of the growth front in both strains, compared with unsupplemented controls. However, this inhibition did not appear to be responsible for the lengthened periods in bd csp cel. Nor was direct incorporation of the short-chain (period-lengthening) fatty acids into mycelial total lipids responsible, since such incorporation was not observed. In fact, extensive metabolic conversion of these supplements by both strains was indicated by the disappearance of short-chain fatty acids from the agar media coupled with their absence in mycelial lipids, and by the liberation of (14)CO(2) from cultures supplemented with [1-(14)C]lauric acid (12:0).
- Published
- 1979
- Full Text
- View/download PDF
94. Morphology and growth kinetics of hyphae of differentiated and undifferentiated mycelia of Neurospora crassa.
- Author
-
Steele GC and Trinci AP
- Subjects
- Culture Media, Models, Biological, Morphogenesis, Neurospora crassa cytology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
A comparison was made of the morphology and growth kinetics of hyphae of differentiated and undifferentiated mycelia of Neurospora crassa. Undifferentiated mycelia were formed during exponential growth on solid media or submerged culture. Hyphae at the margin of differentiated mycelia (colonies) differed from undifferentiated mycelia in diameter, extension rate, extension zone length, and intercalary and apical compartment length. The mean hyphal extension rate (E) of an undifferentiated mycelium was a function of the length of the mycelium's hyphal growth unit (G) and the organism's specific growth rate (alpha). Thus, E=Galpha.
- Published
- 1975
- Full Text
- View/download PDF
95. Unsaturated fatty acid isomers: effects on the circadian rhythm of a fatty-acid-deficient Neurospora crassa mutant.
- Author
-
Mattern DL
- Subjects
- Chemical Phenomena, Chemistry, Fatty Acids deficiency, Isomerism, Mutation, Neurospora crassa genetics, Circadian Rhythm drug effects, Fatty Acids, Unsaturated pharmacology, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
The fatty acids oleic, linoleic, and linolenic, each of which has a cis double bond at the delta 9 position, are known to lengthen the circadian period of conidiation (spore formation) of strains of Neurospora crassa carrying the cel mutation. cel confers a partial fatty acid requirement on the organism and has been used to promote incorporation of exogenous fatty acids. To test whether a physical effect imparted by the cis double bonds, such as increased membrane fluidity, is critical for the perturbation of the rhythm, various isomers of these fatty acids were supplemented to the bd csp cel strain. Positional isomers of oleic acid, such as petroselinic (delta 6) and vaccenic (delta 11) acids, and longer-chain isomers, such as eicosenoic (delta 11) and erucic (delta 13) acids, did not lengthen the rhythm. The shorter-chain palmitoleic (delta 9) acid did not give a consistent lengthening of the rhythm; it may be elongated to vaccenic acid. In contrast, gamma-linolenic acid (delta 6,9,12) dramatically lengthened the period. Linoelaidic acid (the trans,trans isomer of linoleic acid) lengthened the period at 22 degrees C, but elaidic acid (the trans isomer of oleic acid) did not. Elaidic acid was shown to exert a lengthening effect, but only at lower temperatures. The data do not support a direct physical action as the source of the fatty acids' "chronobiotic" ability.
- Published
- 1985
- Full Text
- View/download PDF
96. Hyphal wall growth in Neurospora crassa and Geotrichum candidum.
- Author
-
Trinci AP and Collinge AJ
- Subjects
- Cell Wall ultrastructure, Geotrichum ultrastructure, Models, Biological, Neurospora crassa ultrastructure, Geotrichum growth & development, Mitosporic Fungi growth & development, Neurospora growth & development, Neurospora crassa growth & development
- Abstract
Growth of the walls of hyphae of Neurospora crassa and Geotrichum candidum was studied using longitudinal and serial transverse sectioning methods. Rigidification of the hyphal wall below the extension zone did not appear to involve the gross formation of a secondary wall since the transition from extensible to non-extensible wall was not associated with an increase in thickness. However, behind the extension zone the walls leading hyphae of N. crassa increased in thickness until eventually they attained a thickness which was up to five times that of the tip wall. A hypothesis of hyphal wall growth is proposed.
- Published
- 1975
- Full Text
- View/download PDF
97. Physiological response of Neurospora conidia to freezing in the dehydrated, hydrated, or germinated state.
- Author
-
Leef JL and Mazur P
- Subjects
- Oxygen Consumption, Spores, Fungal growth & development, Spores, Fungal metabolism, Sucrose, Culture Media, Desiccation, Freezing, Neurospora growth & development, Neurospora crassa growth & development, Neurospora crassa metabolism, Water
- Abstract
This study concerned the response to freezing of Neurospora crassa conidia in four different states: air-dry, hydrated in water, hydrated in Vogel medium lacking only sucrose, or hydrated in complete Vogel medium. All hydrated conidia were incubated in one of the above media for various times before freezing and were then washed and frozen in distilled water. Viability was estimated by three techniques, and the agreement among them was good. Hydration of air-dry conidia was found to be very rapid and, once hydrated, the conidia were much more sensitive to rapid freezing than they were before hydration. Rapidly cooled conidia survived freezing to a much higher extent when the warming rate was rapid than when it was slow; slowly cooled conidia showed little or no dependence on the warming rate. This sensitivity to rapid cooling and slow warming was attributed to the effects of intracellular ice. The sensitivity to freezing could be reversed by dehydrating the conidia in vacuo before freezing; thus, it was concluded that the presence or absence of water is the determining factor in the initial sensitivity due to freezing. In water, the sensitivity remained constant from 2 min to 15 days after hydration. Although conidia hydrated in growth medium lacking sucrose remained metabolically inactive, their sensitivity to rapid freezing decreased as a function of time in the medium before freezing. The reason for this decreased sensitivity is not understood. Conidia hydrated in complete growth medium (i.e., containing sucrose) became metabolically active and, after the initial sensitivity associated with hydration, became increasingly more sensitive to freezing as a function of their time in the medium. Drying itself was deleterious to metabolically active conidia, and those that survived dehydration did not exhibit a large absolute increase in resistance to subsequent freezing. The increase in sensitivity to freezing and to drying seems associated with the presence of metabolic activity; however, the precise cause of the sensitization remains obscure.
- Published
- 1978
- Full Text
- View/download PDF
98. NH 2 -terminal residues of Neurospora crassa proteins.
- Author
-
Rho HM and DeBusk AG
- Subjects
- Alanine analysis, Autoanalysis, Cell-Free System, Chromatography, Paper, Culture Media, Dinitrophenols, Glycine analysis, Hydrolysis, Neurospora classification, Neurospora growth & development, Phenylalanine analysis, Proteins isolation & purification, Serine analysis, Spectrophotometry, Amino Acids analysis, Neurospora analysis, Proteins analysis
- Abstract
The NH(2)-terminal amino acid composition of the soluble and ribosomal proteins from Neurospora crassa mycelia and conidia was determined by the dinitrophenyl method. A nonrandom distribution of NH(2)-terminal amino acids was observed in the complex protein mixtures. Glycine, alanine, and serine accounted for 75% of the NH(2)-terminal amino acids, and glycine appeared most frequently in mature proteins of mycelia. The appearance of phenylalanine as one of the major NH(2)-termini in crude conidial fraction suggests that the composition of proteins may vary in different developmental stages.
- Published
- 1971
- Full Text
- View/download PDF
99. Sugar transport in Neurospora crassa. 3. An inositol requirement for the function of the glucose active transport system.
- Author
-
Scarborough GA
- Subjects
- Adenosine Triphosphate analysis, Carbon Isotopes, Fructose pharmacology, Inositol metabolism, Inositol pharmacology, Lipids biosynthesis, Neurospora analysis, Neurospora drug effects, Neurospora growth & development, Potassium analysis, Tritium, Biological Transport, Active drug effects, Glucose metabolism, Neurospora metabolism, Sorbose metabolism
- Published
- 1971
- Full Text
- View/download PDF
100. [Composition of the amino acid pool of Neurospora in deficiency of growth substance].
- Author
-
Aurich H
- Subjects
- Alanine analysis, Amino Acids analysis, Ammonia analysis, Arginine analysis, Aspartic Acid analysis, Biotin, Choline, Chromatography, Ion Exchange, Culture Media, Glutamates analysis, Glycine analysis, Lysine analysis, Methionine analysis, Mutation, Phenylalanine analysis, Pyridoxine, Riboflavin, Serine analysis, Threonine analysis, Tyrosine analysis, Valine analysis, Amino Acids metabolism, Growth Inhibitors, Neurospora growth & development
- Published
- 1966
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.