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Preparation of functional group analogs of unsaturated fatty acids and their effects on the circadian rhythm of a fatty-acid-deficient mutant of Neurospora crassa.
- Source :
-
Chemistry and physics of lipids [Chem Phys Lipids] 1985 Jun-Jul; Vol. 37 (3), pp. 297-306. - Publication Year :
- 1985
-
Abstract
- Functional group analogs of oleic, linoleic and linolenic acids were prepared by coverting their double bonds to dibromo, cyclopropyl, epoxy, methoxy, and, in the case of oleic acid, hydroxy groups. These compounds were supplemented to the bd csp cel strain of the mold Neurospora crassa. The cel mutation confers a partial requirement for saturated fatty acids and, also, perturbs the circadian rhythm of spore formation. For example, the period of bd csp cel's rhythm is dramatically lengthened upon supplementation by natural cis-unsaturated fatty acids. Of the analogs tested, only the monoepoxy, monomethoxy, dibromo, and hexabromo stearic acids gave significant period lengthening. Other analogs, which should have comparable abilities to disrupt lipid bilayer packing, gave no rhythm effect. Further, the inactive di- and tri-methoxystearic acid analogs were incorporated to a greater extent than the active mono-methoxystearic acid. The results do not, therefore, support a direct alteration in membrane "fluidity' as the mode of action of the period-lengthening fatty acids.
Details
- Language :
- English
- ISSN :
- 0009-3084
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Chemistry and physics of lipids
- Publication Type :
- Academic Journal
- Accession number :
- 2931204
- Full Text :
- https://doi.org/10.1016/0009-3084(85)90017-9