1. Colloidal Crystals Delay Formation of Early Stage Bacterial Biofilms
- Author
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Mehdi Kargar, Hamoun Khalili Hoseinabad, William A. Ducker, Amy Pruden, and Yow-Ren Chang
- Subjects
Fluorescence-lifetime imaging microscopy ,Materials science ,Biomedical Engineering ,Biofilm ,Nanotechnology ,02 engineering and technology ,engineering.material ,Colloidal crystal ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,Biomaterials ,chemistry.chemical_compound ,Adsorption ,Chemical engineering ,Coating ,chemistry ,law ,engineering ,Polystyrene ,Electron microscope ,0210 nano-technology ,Layer (electronics) - Abstract
The objective of this work was to examine whether close-packed spheres of polystyrene (colloidal crystals) could be used to delay the development of biofilms. We examined early stage biofilm formation of Pseudomonas aeruginosa after 2 days on a flat sheet of polystyrene and on the same solid coated in polystyrene spheres of 450 or 1500 nm diameter. All materials were coated in fetal bovine serum to enable comparison of the effects of different surface curvature while maintaining constant surface chemistry. After 2 days, fluorescence imaging showed that the volume of bacterial colonies was much smaller on the 1500 nm colloidal crystals than on the flat film. In addition, electron microscopy showed that the area covered by structures containing more than one layer of bacteria was significantly reduced on both the 450 and 1500 nm colloidal crystals compared to the flat sheet. This provides proof of concept of biofilm inhibition of a pathogen by a simple nonchemical coating that may find future application in... more...
- Published
- 2021