1. Allulose Attenuated Age-Associated Sarcopenia via Regulating IGF-1 and Myostatin in Aged Mice
- Author
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Jieun Kim, Eun-Young Kwon, and Youngji Han
- Subjects
Programmed cell death ,medicine.medical_specialty ,Sarcopenia ,medicine.medical_treatment ,Mice, Obese ,Myostatin ,Fructose ,Protein degradation ,Mice ,Internal medicine ,medicine ,Animals ,Insulin-Like Growth Factor I ,Muscle, Skeletal ,PI3K/AKT/mTOR pathway ,Mammals ,Adiponectin ,biology ,Chemistry ,Growth factor ,Autophagy ,medicine.disease ,Endocrinology ,biology.protein ,Food Science ,Biotechnology - Abstract
SCOPE Allulose is shown to increase the muscle weight in diet-induced obese mice. However, there are no studies on the effects of allulose in age-associated sarcopenia. This study aims to elucidate the mechanisms of action for allulose in age associated by analyzing the transcriptional patterns in aged mice. METHODS AND RESULTS The 48-week-old mice are fed with AIN-93diet containing allulose for 12 weeks. Allulose supplementation increases the muscle mass and grip strength in aged mice. Allulose increases the insulin-like growth factor 1 (IGF-1) and its downstream factor expressions which 40 are related protein synthesis, while inhibits the myostatin expression related protein degradation. In mRNA-seq analysis, allulose supplementation significantly decreases in Adiponectin, Adipsin, cell death inducing DFFA like effector (CIDEC), Haptoglobin, Neuroglobin, and stearoyl-CoA desaturase-1 (SCD1) and increases in cytokine-inducible SH2-containing protein (CISH) and ceramide synthase 1 (CerS1) that are regulate protein turn over in gastrocnemius. Also, allulose alleviates autophagy in muscle with regulated mammalian target of rapamycin (mTOR) signaling pathway and increases the anti-oxidant enzyme activity. CONCLUSION These findings suggest that allulose improves the age-associated sarcopenia with enhancing antioxidant properties by altering mRNA and protein expression.
- Published
- 2021