1. AQP4-siRNA alleviates traumatic brain edema by altering post-traumatic AQP4 polarity reversal in TBI rats
- Author
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Jianqiang Chen, Haixia Chen, Hong Lu, Li Ai, and Yuefu Zhan
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Traumatic brain injury ,Brain Edema ,Cerebral edema ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,Edema ,Brain Injuries, Traumatic ,Extracellular ,Animals ,Medicine ,Distribution (pharmacology) ,RNA, Small Interfering ,Pathological ,Aquaporin 4 ,business.industry ,General Medicine ,medicine.disease ,Rats ,nervous system diseases ,medicine.anatomical_structure ,Neurology ,Astrocytes ,Brain Injuries ,030220 oncology & carcinogenesis ,Surgery ,sense organs ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Intracellular ,Astrocyte - Abstract
The spatial and temporal distribution of aquaporin-4 (AQP4) expression in rat brain following brain trauma and AQP4-siRNA treatment, as well as corresponding pathological changes, were studied to explore the mechanism underlying the effect of AQP4-siRNA treatment on traumatic brain injury (TBI). The rats in the sham operation group had normal structure, with AQP4 located in the perivascular end-foot membranes and astrocytic membranes in a polarized pattern. The accelerated polarity reversal was observed in the TBI group in 1–12 h after TBI. During this period, AQP4 abundance on the astrocytic membrane is gradually increased, while AQP4 abundance on the perivascular end-foot membrane declined rapidly. Twelve hours after TBI, AQP4 expression was depolarized, showing a shift from the perivascular end-foot membrane to the astrocytic membrane. Pathological observation showed that vasogenic edema occurred immediately after TBI, at which time the extracellular space was expanded, leading to severe intracellular edema. AQP4-siRNA reduced the polarity reversal index at the early stage of TBI recovery and reduced edema, demonstrating the potential benefit of reduced AQP4 expression during recovery from TBI.
- Published
- 2020