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AQP4-siRNA alleviates traumatic brain edema by altering post-traumatic AQP4 polarity reversal in TBI rats
- Source :
- Journal of Clinical Neuroscience. 81:113-119
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The spatial and temporal distribution of aquaporin-4 (AQP4) expression in rat brain following brain trauma and AQP4-siRNA treatment, as well as corresponding pathological changes, were studied to explore the mechanism underlying the effect of AQP4-siRNA treatment on traumatic brain injury (TBI). The rats in the sham operation group had normal structure, with AQP4 located in the perivascular end-foot membranes and astrocytic membranes in a polarized pattern. The accelerated polarity reversal was observed in the TBI group in 1–12 h after TBI. During this period, AQP4 abundance on the astrocytic membrane is gradually increased, while AQP4 abundance on the perivascular end-foot membrane declined rapidly. Twelve hours after TBI, AQP4 expression was depolarized, showing a shift from the perivascular end-foot membrane to the astrocytic membrane. Pathological observation showed that vasogenic edema occurred immediately after TBI, at which time the extracellular space was expanded, leading to severe intracellular edema. AQP4-siRNA reduced the polarity reversal index at the early stage of TBI recovery and reduced edema, demonstrating the potential benefit of reduced AQP4 expression during recovery from TBI.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Traumatic brain injury
Brain Edema
Cerebral edema
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
Edema
Brain Injuries, Traumatic
Extracellular
Animals
Medicine
Distribution (pharmacology)
RNA, Small Interfering
Pathological
Aquaporin 4
business.industry
General Medicine
medicine.disease
Rats
nervous system diseases
medicine.anatomical_structure
Neurology
Astrocytes
Brain Injuries
030220 oncology & carcinogenesis
Surgery
sense organs
Neurology (clinical)
medicine.symptom
business
030217 neurology & neurosurgery
Intracellular
Astrocyte
Subjects
Details
- ISSN :
- 09675868
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Neuroscience
- Accession number :
- edsair.doi.dedup.....4c184d11c097cee98669f578a75a94ef