1. Chitosan nanoparticles plus quercetin suppress the oxidative stress, modulate DNA fragmentation and gene expression in the kidney of rats fed ochratoxin A-contaminated diet
- Author
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Abdulhadi Aljawish, Mosaad A. Abdel-Wahhab, Nabila S. Hassan, Sekena H. Abdel-Aziem, Aziza A. El-Nekeety, National Research Center ( NRC ), National Research Center, Equipe NuTox (LNC - U1231) ( NUTOX ), Lipides - Nutrition - Cancer [Dijon - U1231] ( LNC ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Santé et de la Recherche Médicale ( INSERM )
- Subjects
0301 basic medicine ,Ochratoxin A ,Male ,MESH : Oxidative Stress ,MESH : Carcinogens ,Antioxidant ,medicine.medical_treatment ,[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition ,[ SDV.TOX ] Life Sciences [q-bio]/Toxicology ,Pharmacology ,Toxicology ,medicine.disease_cause ,Kidney ,Antioxidants ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Malondialdehyde ,MESH : Ochratoxins ,MESH : Protective Agents ,MESH : DNA Fragmentation ,Nephrotoxicity ,Chemistry ,MESH : Rats ,General Medicine ,Ochratoxins ,MESH : Kidney ,Drug Combinations ,medicine.anatomical_structure ,MESH : Drug Combinations ,Biochemistry ,Liver ,030220 oncology & carcinogenesis ,MESH : Quercetin ,DNA fragmentation ,MESH : Antioxidants ,Quercetin ,MESH : Chitosan ,MESH : Male ,Food Contamination ,DNA Fragmentation ,Protective Agents ,MESH : Diet ,03 medical and health sciences ,medicine ,Animals ,MESH : Malondialdehyde ,Chitosan ,technology, industry, and agriculture ,MESH : Liver ,Mycotoxins ,Chitosan nanoparticles ,MESH : Rats, Sprague-Dawley ,Diet ,Rats ,MESH : Food Contamination ,030104 developmental biology ,Oxidative stress ,MESH : Nanoparticles ,Carcinogens ,Nanoparticles ,MESH : Animals ,Genotoxicity ,Food Science - Abstract
IF 3.778; International audience; This study aimed to evaluate the protective role of chitosan nanoparticles (COS-NPs) singly or plus quercetin (Q) against OTA-induced oxidative stress and renal genotoxicity. Twelve groups of male Sprague-Dawley rats were treated orally for 3 weeks included the control group, animals fed OTA-contaminated diet (3 mg/kg diet); COS-NPs-treated groups at low (140 mg/kg b.w.) or high (280 mg/kg b.w.) dose, Q-treated group (50 mg/kg b.w.), Q plus low or high dose of COS-NPs-treated groups and OTA plus Q and/or COS-NPs at the two tested doses-treated groups. The results indicated that COS-NPs were roughly rod in shape with average particle size of 200 nm and zeta potential 31.4 ± 2.8 mV. Animals fed OTA-contaminated diet showed significant changes in serum biochemical parameters, increase kidney MDA and DNA fragmentation and decreased GPx and SOD gene expression accompanied with severe histological changes. Q and/or COS-NPs at the two tested doses induced significant improvements in all tested parameters and succeeded to overcome these effects especially in the animals treated with Q plus the high dose of COS-NPs. It could be concluded that COS-NPs are promise candidate to enhance the antioxidant effect of Q and protect against the nephrotoxicity of OTA in high endemic areas.
- Published
- 2017