1. Nanozyme chemiluminescence paper test for rapid and sensitive detection of SARS-CoV-2 antigen
- Author
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Chenhui Ju, Jinghua Yan, Demin Duan, Chao Han, Dan Liu, Xuehui Chen, Rui Shi, and Xiyun Yan
- Subjects
Antigen detection ,Chemiluminescence ,Point-of-care testing ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,01 natural sciences ,Article ,law.invention ,Antigen ,law ,Electrochemistry ,Detection limit ,biology ,SARS-CoV-2 ,Chemistry ,010401 analytical chemistry ,Nanozyme ,General Medicine ,Gold standard (test) ,021001 nanoscience & nanotechnology ,Virology ,0104 chemical sciences ,Linear range ,Nucleic acid ,biology.protein ,Paper test ,Antibody ,0210 nano-technology ,Biotechnology - Abstract
COVID-19 has evolved into a global pandemic. Early and rapid detection is crucial to control of the SARS-CoV-2 transmission. While representing the gold standard for early diagnosis, nucleic acid tests for SARS-CoV-2 are often complicated and time-consuming. Serological rapid antibody tests are characterized by high rates of false-negative diagnoses, especially during early infection. Here, we developed a novel nanozyme-based chemiluminescence paper assay for rapid and sensitive detection of SARS-CoV-2 spike antigen, which integrates nanozyme and enzymatic chemiluminescence immunoassay with the lateral flow strip. The core of our paper test is a robust Co–Fe@hemin-peroxidase nanozyme that catalyzes chemiluminescence comparable with natural peroxidase HRP and thus amplifies immune reaction signal. The detection limit for recombinant spike antigen of SARS-CoV-2 was 0.1 ng/mL, with a linear range of 0.2-100 ng/mL. Moreover, the sensitivity of test for pseudovirus could reach 360 TCID50/mL, which was comparable with ELISA method. The strip recognized SARS-CoV-2 antigen specifically, and there was no cross reaction with other coronaviruses or influenza A subtypes. This testing can be completed within 16 min, much shorter compared to the usual 1-2 h required for currently used nucleic acid tests. Furthermore, signal detection is feasible using the camera of a standard smartphone. Ingredients for nanozyme synthesis are simple and readily available, considerably lowering the overall cost. In conclusion, our paper test provides a high-sensitive point-of-care testing (POCT) approach for SARS-CoV-2 antigen detection, which should greatly facilitate early screening of SARS-CoV-2 infections, and considerably lower the financial burden on national healthcare resources., Highlights • Point-of-care antigen tests for screening of SARS-CoV-2 infection were established. • Chemiluminescence improves sensitivity of paper test and benefits early screening. • Nanozyme with high peroxidase activity and stability catalyzes chemiluminescence. • Nanozyme chemiluminescence paper test brings high efficiency and low cost.
- Published
- 2021